Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066802.t1 | AES72095.1 | 95.2 | 147 | 7 | 0 | 1 | 147 | 1 | 147 | 1.10E-73 | 285.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066802.t1 | Q00445 | 33.3 | 135 | 73 | 4 | 19 | 146 | 34 | 158 | 6.8e-11 | 68.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066802.t1 | G7J2I8 | 95.2 | 147 | 7 | 0 | 1 | 147 | 1 | 147 | 8.2e-74 | 285.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene066802 | 0.843522 | 1.33E-58 | -1.69E-46 |
| MS.gene049171 | MS.gene066802 | 0.830633 | 2.63E-55 | -1.69E-46 |
| MS.gene049217 | MS.gene066802 | 0.82074 | 5.85E-53 | -1.69E-46 |
| MS.gene049222 | MS.gene066802 | 0.865359 | 6.25E-65 | -1.69E-46 |
| MS.gene049232 | MS.gene066802 | 0.825671 | 4.13E-54 | -1.69E-46 |
| MS.gene049285 | MS.gene066802 | 0.832818 | 7.61E-56 | -1.69E-46 |
| MS.gene049320 | MS.gene066802 | 0.840663 | 7.59E-58 | -1.69E-46 |
| MS.gene049369 | MS.gene066802 | 0.807727 | 4.39E-50 | -1.69E-46 |
| MS.gene049396 | MS.gene066802 | 0.856912 | 2.33E-62 | -1.69E-46 |
| MS.gene049427 | MS.gene066802 | 0.80703 | 6.17E-50 | -1.69E-46 |
| MS.gene049609 | MS.gene066802 | 0.893981 | 3.79E-75 | -1.69E-46 |
| MS.gene049633 | MS.gene066802 | 0.816223 | 6.18E-52 | -1.69E-46 |
| MS.gene049656 | MS.gene066802 | 0.801373 | 9.28E-49 | -1.69E-46 |
| MS.gene049805 | MS.gene066802 | 0.855867 | 4.73E-62 | -1.69E-46 |
| MS.gene05010 | MS.gene066802 | 0.824437 | 8.08E-54 | -1.69E-46 |
| MS.gene050170 | MS.gene066802 | 0.813927 | 2.00E-51 | -1.69E-46 |
| MS.gene050323 | MS.gene066802 | 0.815787 | 7.73E-52 | -1.69E-46 |
| MS.gene050499 | MS.gene066802 | 0.809264 | 2.06E-50 | -1.69E-46 |
| MS.gene050502 | MS.gene066802 | 0.841552 | 4.43E-58 | -1.69E-46 |
| MS.gene050504 | MS.gene066802 | 0.808665 | 2.77E-50 | -1.69E-46 |
| MS.gene050518 | MS.gene066802 | 0.823422 | 1.40E-53 | -1.69E-46 |
| MS.gene050538 | MS.gene066802 | 0.863812 | 1.90E-64 | -1.69E-46 |
| MS.gene050577 | MS.gene066802 | 0.830114 | 3.52E-55 | -1.69E-46 |
| MS.gene050593 | MS.gene066802 | 0.848083 | 7.68E-60 | -1.69E-46 |
| MS.gene050612 | MS.gene066802 | 0.80503 | 1.63E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene066802 | 0.805281 | 1.44E-49 | -1.69E-46 |
| MS.gene050686 | MS.gene066802 | 0.823893 | 1.08E-53 | -1.69E-46 |
| MS.gene050690 | MS.gene066802 | 0.803882 | 2.82E-49 | -1.69E-46 |
| MS.gene050699 | MS.gene066802 | 0.82603 | 3.39E-54 | -1.69E-46 |
| MS.gene050747 | MS.gene066802 | 0.882013 | 1.49E-70 | -1.69E-46 |
| MS.gene050772 | MS.gene066802 | 0.895933 | 5.98E-76 | -1.69E-46 |
| MS.gene05080 | MS.gene066802 | 0.84004 | 1.10E-57 | -1.69E-46 |
| MS.gene05081 | MS.gene066802 | 0.80759 | 4.69E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene066802 | 0.810525 | 1.10E-50 | -1.69E-46 |
| MS.gene050871 | MS.gene066802 | 0.808772 | 2.63E-50 | -1.69E-46 |
| MS.gene050950 | MS.gene066802 | 0.872793 | 2.42E-67 | -1.69E-46 |
| MS.gene05100 | MS.gene066802 | 0.800795 | 1.22E-48 | -1.69E-46 |
| MS.gene05105 | MS.gene066802 | 0.850635 | 1.49E-60 | -1.69E-46 |
| MS.gene051167 | MS.gene066802 | -0.801621 | 8.26E-49 | -1.69E-46 |
| MS.gene051387 | MS.gene066802 | 0.835257 | 1.86E-56 | -1.69E-46 |
| MS.gene051551 | MS.gene066802 | 0.81384 | 2.09E-51 | -1.69E-46 |
| MS.gene051666 | MS.gene066802 | 0.86122 | 1.20E-63 | -1.69E-46 |
| MS.gene051692 | MS.gene066802 | 0.800087 | 1.70E-48 | -1.69E-46 |
| MS.gene051734 | MS.gene066802 | 0.808772 | 2.63E-50 | -1.69E-46 |
| MS.gene051838 | MS.gene066802 | 0.851707 | 7.45E-61 | -1.69E-46 |
| MS.gene051858 | MS.gene066802 | 0.808361 | 3.22E-50 | -1.69E-46 |
| MS.gene052164 | MS.gene066802 | 0.850726 | 1.41E-60 | -1.69E-46 |
| MS.gene052346 | MS.gene066802 | 0.850262 | 1.90E-60 | -1.69E-46 |
| MS.gene052414 | MS.gene066802 | 0.85977 | 3.29E-63 | -1.69E-46 |
| MS.gene052421 | MS.gene066802 | 0.846688 | 1.86E-59 | -1.69E-46 |
| MS.gene052481 | MS.gene066802 | 0.823837 | 1.12E-53 | -1.69E-46 |
| MS.gene052482 | MS.gene066802 | 0.813473 | 2.51E-51 | -1.69E-46 |
| MS.gene052484 | MS.gene066802 | 0.818117 | 2.32E-52 | -1.69E-46 |
| MS.gene052486 | MS.gene066802 | 0.824419 | 8.16E-54 | -1.69E-46 |
| MS.gene052501 | MS.gene066802 | 0.875687 | 2.53E-68 | -1.69E-46 |
| MS.gene052503 | MS.gene066802 | 0.879977 | 8.02E-70 | -1.69E-46 |
| MS.gene052505 | MS.gene066802 | 0.866885 | 2.06E-65 | -1.69E-46 |
| MS.gene052506 | MS.gene066802 | 0.851095 | 1.11E-60 | -1.69E-46 |
| MS.gene052615 | MS.gene066802 | 0.853456 | 2.36E-61 | -1.69E-46 |
| MS.gene052820 | MS.gene066802 | 0.81469 | 1.36E-51 | -1.69E-46 |
| MS.gene052822 | MS.gene066802 | 0.854301 | 1.35E-61 | -1.69E-46 |
| MS.gene052823 | MS.gene066802 | 0.873164 | 1.82E-67 | -1.69E-46 |
| MS.gene052825 | MS.gene066802 | 0.868197 | 7.81E-66 | -1.69E-46 |
| MS.gene052916 | MS.gene066802 | 0.809073 | 2.27E-50 | -1.69E-46 |
| MS.gene052966 | MS.gene066802 | 0.804865 | 1.76E-49 | -1.69E-46 |
| MS.gene052982 | MS.gene066802 | 0.86204 | 6.71E-64 | -1.69E-46 |
| MS.gene053104 | MS.gene066802 | 0.828202 | 1.02E-54 | -1.69E-46 |
| MS.gene053203 | MS.gene066802 | 0.841091 | 5.86E-58 | -1.69E-46 |
| MS.gene053207 | MS.gene066802 | 0.846242 | 2.46E-59 | -1.69E-46 |
| MS.gene053213 | MS.gene066802 | 0.879048 | 1.71E-69 | -1.69E-46 |
| MS.gene053286 | MS.gene066802 | 0.870656 | 1.24E-66 | -1.69E-46 |
| MS.gene053436 | MS.gene066802 | 0.882363 | 1.11E-70 | -1.69E-46 |
| MS.gene053449 | MS.gene066802 | 0.819568 | 1.08E-52 | -1.69E-46 |
| MS.gene053480 | MS.gene066802 | 0.825946 | 3.55E-54 | -1.69E-46 |
| MS.gene053574 | MS.gene066802 | 0.905672 | 3.35E-80 | -1.69E-46 |
| MS.gene053579 | MS.gene066802 | 0.802642 | 5.09E-49 | -1.69E-46 |
| MS.gene053626 | MS.gene066802 | 0.859635 | 3.61E-63 | -1.69E-46 |
| MS.gene053632 | MS.gene066802 | 0.841077 | 5.91E-58 | -1.69E-46 |
| MS.gene053824 | MS.gene066802 | 0.837547 | 4.87E-57 | -1.69E-46 |
| MS.gene053916 | MS.gene066802 | 0.833787 | 4.36E-56 | -1.69E-46 |
| MS.gene054003 | MS.gene066802 | 0.81284 | 3.46E-51 | -1.69E-46 |
| MS.gene054408 | MS.gene066802 | 0.84825 | 6.91E-60 | -1.69E-46 |
| MS.gene054595 | MS.gene066802 | 0.823449 | 1.38E-53 | -1.69E-46 |
| MS.gene054600 | MS.gene066802 | 0.847046 | 1.48E-59 | -1.69E-46 |
| MS.gene054642 | MS.gene066802 | 0.848556 | 5.68E-60 | -1.69E-46 |
| MS.gene054706 | MS.gene066802 | -0.800101 | 1.69E-48 | -1.69E-46 |
| MS.gene05470 | MS.gene066802 | 0.801796 | 7.60E-49 | -1.69E-46 |
| MS.gene054927 | MS.gene066802 | 0.867074 | 1.79E-65 | -1.69E-46 |
| MS.gene054954 | MS.gene066802 | 0.816079 | 6.65E-52 | -1.69E-46 |
| MS.gene05505 | MS.gene066802 | 0.863115 | 3.13E-64 | -1.69E-46 |
| MS.gene055206 | MS.gene066802 | 0.82416 | 9.38E-54 | -1.69E-46 |
| MS.gene055212 | MS.gene066802 | 0.808031 | 3.78E-50 | -1.69E-46 |
| MS.gene055224 | MS.gene066802 | 0.825748 | 3.96E-54 | -1.69E-46 |
| MS.gene055289 | MS.gene066802 | 0.865906 | 4.20E-65 | -1.69E-46 |
| MS.gene055362 | MS.gene066802 | 0.839376 | 1.64E-57 | -1.69E-46 |
| MS.gene055486 | MS.gene066802 | 0.824943 | 6.14E-54 | -1.69E-46 |
| MS.gene055502 | MS.gene066802 | 0.848575 | 5.62E-60 | -1.69E-46 |
| MS.gene055577 | MS.gene066802 | 0.844078 | 9.44E-59 | -1.69E-46 |
| MS.gene055722 | MS.gene066802 | 0.857402 | 1.67E-62 | -1.69E-46 |
| MS.gene055741 | MS.gene066802 | 0.8077 | 4.45E-50 | -1.69E-46 |
| MS.gene055744 | MS.gene066802 | 0.803509 | 3.37E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene066802 | 0.824409 | 8.20E-54 | -1.69E-46 |
| MS.gene055909 | MS.gene066802 | 0.832844 | 7.50E-56 | -1.69E-46 |
| MS.gene055911 | MS.gene066802 | 0.829492 | 5.00E-55 | -1.69E-46 |
| MS.gene05592 | MS.gene066802 | 0.802917 | 4.47E-49 | -1.69E-46 |
| MS.gene055941 | MS.gene066802 | 0.817757 | 2.79E-52 | -1.69E-46 |
| MS.gene056040 | MS.gene066802 | 0.809872 | 1.53E-50 | -1.69E-46 |
| MS.gene056046 | MS.gene066802 | 0.817821 | 2.70E-52 | -1.69E-46 |
| MS.gene056166 | MS.gene066802 | 0.87159 | 6.09E-67 | -1.69E-46 |
| MS.gene056374 | MS.gene066802 | 0.814778 | 1.30E-51 | -1.69E-46 |
| MS.gene056422 | MS.gene066802 | 0.823886 | 1.09E-53 | -1.69E-46 |
| MS.gene056446 | MS.gene066802 | 0.818157 | 2.27E-52 | -1.69E-46 |
| MS.gene056448 | MS.gene066802 | 0.829427 | 5.18E-55 | -1.69E-46 |
| MS.gene056487 | MS.gene066802 | 0.851255 | 9.99E-61 | -1.69E-46 |
| MS.gene056504 | MS.gene066802 | 0.842965 | 1.87E-58 | -1.69E-46 |
| MS.gene056510 | MS.gene066802 | 0.810567 | 1.08E-50 | -1.69E-46 |
| MS.gene056511 | MS.gene066802 | 0.857718 | 1.35E-62 | -1.69E-46 |
| MS.gene056610 | MS.gene066802 | 0.801565 | 8.48E-49 | -1.69E-46 |
| MS.gene05664 | MS.gene066802 | 0.82832 | 9.60E-55 | -1.69E-46 |
| MS.gene056657 | MS.gene066802 | 0.854111 | 1.53E-61 | -1.69E-46 |
| MS.gene05666 | MS.gene066802 | 0.829204 | 5.87E-55 | -1.69E-46 |
| MS.gene056692 | MS.gene066802 | 0.809475 | 1.86E-50 | -1.69E-46 |
| MS.gene057048 | MS.gene066802 | 0.803964 | 2.71E-49 | -1.69E-46 |
| MS.gene057076 | MS.gene066802 | 0.870654 | 1.24E-66 | -1.69E-46 |
| MS.gene057086 | MS.gene066802 | 0.82996 | 3.84E-55 | -1.69E-46 |
| MS.gene057124 | MS.gene066802 | 0.80473 | 1.88E-49 | -1.69E-46 |
| MS.gene057331 | MS.gene066802 | 0.834113 | 3.62E-56 | -1.69E-46 |
| MS.gene057416 | MS.gene066802 | 0.86458 | 1.10E-64 | -1.69E-46 |
| MS.gene057658 | MS.gene066802 | 0.818829 | 1.60E-52 | -1.69E-46 |
| MS.gene057734 | MS.gene066802 | 0.859759 | 3.31E-63 | -1.69E-46 |
| MS.gene057823 | MS.gene066802 | 0.801586 | 8.40E-49 | -1.69E-46 |
| MS.gene057831 | MS.gene066802 | 0.817113 | 3.90E-52 | -1.69E-46 |
| MS.gene057856 | MS.gene066802 | 0.829356 | 5.39E-55 | -1.69E-46 |
| MS.gene05787 | MS.gene066802 | 0.823367 | 1.44E-53 | -1.69E-46 |
| MS.gene057926 | MS.gene066802 | 0.80532 | 1.41E-49 | -1.69E-46 |
| MS.gene058019 | MS.gene066802 | 0.847826 | 9.04E-60 | -1.69E-46 |
| MS.gene058130 | MS.gene066802 | 0.839443 | 1.58E-57 | -1.69E-46 |
| MS.gene058338 | MS.gene066802 | 0.81153 | 6.68E-51 | -1.69E-46 |
| MS.gene058376 | MS.gene066802 | 0.819859 | 9.31E-53 | -1.69E-46 |
| MS.gene05837 | MS.gene066802 | 0.83915 | 1.88E-57 | -1.69E-46 |
| MS.gene058419 | MS.gene066802 | 0.858284 | 9.15E-63 | -1.69E-46 |
| MS.gene058544 | MS.gene066802 | 0.844431 | 7.59E-59 | -1.69E-46 |
| MS.gene058723 | MS.gene066802 | 0.803846 | 2.87E-49 | -1.69E-46 |
| MS.gene058773 | MS.gene066802 | 0.803124 | 4.05E-49 | -1.69E-46 |
| MS.gene058838 | MS.gene066802 | 0.850066 | 2.16E-60 | -1.69E-46 |
| MS.gene05883 | MS.gene066802 | 0.8147 | 1.35E-51 | -1.69E-46 |
| MS.gene058948 | MS.gene066802 | 0.80226 | 6.10E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene066802 | 0.806272 | 8.91E-50 | -1.69E-46 |
| MS.gene059023 | MS.gene066802 | 0.840701 | 7.42E-58 | -1.69E-46 |
| MS.gene05913 | MS.gene066802 | 0.819206 | 1.31E-52 | -1.69E-46 |
| MS.gene05934 | MS.gene066802 | 0.843551 | 1.31E-58 | -1.69E-46 |
| MS.gene059367 | MS.gene066802 | 0.839221 | 1.80E-57 | -1.69E-46 |
| MS.gene059368 | MS.gene066802 | 0.842325 | 2.77E-58 | -1.69E-46 |
| MS.gene059455 | MS.gene066802 | 0.841819 | 3.77E-58 | -1.69E-46 |
| MS.gene059536 | MS.gene066802 | 0.818369 | 2.03E-52 | -1.69E-46 |
| MS.gene059667 | MS.gene066802 | 0.852342 | 4.92E-61 | -1.69E-46 |
| MS.gene059677 | MS.gene066802 | 0.822591 | 2.18E-53 | -1.69E-46 |
| MS.gene059718 | MS.gene066802 | 0.859974 | 2.85E-63 | -1.69E-46 |
| MS.gene059801 | MS.gene066802 | 0.811942 | 5.44E-51 | -1.69E-46 |
| MS.gene059865 | MS.gene066802 | 0.820741 | 5.84E-53 | -1.69E-46 |
| MS.gene059902 | MS.gene066802 | 0.805506 | 1.29E-49 | -1.69E-46 |
| MS.gene060403 | MS.gene066802 | 0.810074 | 1.38E-50 | -1.69E-46 |
| MS.gene060482 | MS.gene066802 | 0.861062 | 1.34E-63 | -1.69E-46 |
| MS.gene060762 | MS.gene066802 | 0.822603 | 2.17E-53 | -1.69E-46 |
| MS.gene060778 | MS.gene066802 | 0.804937 | 1.70E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene066802 | 0.820495 | 6.66E-53 | -1.69E-46 |
| MS.gene060804 | MS.gene066802 | 0.84473 | 6.31E-59 | -1.69E-46 |
| MS.gene060835 | MS.gene066802 | 0.893796 | 4.50E-75 | -1.69E-46 |
| MS.gene060837 | MS.gene066802 | 0.849876 | 2.44E-60 | -1.69E-46 |
| MS.gene06092 | MS.gene066802 | 0.865258 | 6.73E-65 | -1.69E-46 |
| MS.gene06097 | MS.gene066802 | 0.833345 | 5.63E-56 | -1.69E-46 |
| MS.gene060982 | MS.gene066802 | 0.842203 | 2.98E-58 | -1.69E-46 |
| MS.gene061041 | MS.gene066802 | 0.830676 | 2.57E-55 | -1.69E-46 |
| MS.gene061200 | MS.gene066802 | 0.800039 | 1.74E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066802.t1 | MTR_3g086050 | 95.238 | 147 | 7 | 0 | 1 | 147 | 1 | 147 | 2.44e-84 | 246 |
| MS.gene066802.t1 | MTR_3g052760 | 40.310 | 129 | 63 | 5 | 20 | 147 | 34 | 149 | 1.34e-23 | 92.4 |
| MS.gene066802.t1 | MTR_3g092460 | 38.554 | 83 | 34 | 1 | 64 | 146 | 95 | 160 | 2.72e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066802.t1 | AT4G27670 | 40.404 | 99 | 35 | 4 | 56 | 146 | 72 | 154 | 3.67e-14 | 67.4 |
Find 45 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCTGCACCGATAGGGTTA+TGG | 0.285020 | 3.1:+68926145 | MS.gene066802:CDS |
| AAGGAGTAGGTGGGTATAGA+AGG | 0.294040 | 3.1:+68926283 | MS.gene066802:CDS |
| ACAAAGAGGATGTTAAAGTT+TGG | 0.296691 | 3.1:+68926379 | MS.gene066802:CDS |
| CACTCTTTATCCTTAAATCT+TGG | 0.324147 | 3.1:+68925967 | MS.gene066802:CDS |
| TGCTGCACCGATAGGGTTAT+GGG | 0.365142 | 3.1:+68926146 | MS.gene066802:CDS |
| CAAAGAGGATGTTAAAGTTT+GGG | 0.367241 | 3.1:+68926380 | MS.gene066802:CDS |
| TTCTCTTGCTTCACCTGTCA+TGG | 0.368553 | 3.1:-68926052 | None:intergenic |
| CAGGTTAAGACCAAGATTTA+AGG | 0.391199 | 3.1:-68925977 | None:intergenic |
| AGTTTGTCTTGATGAACTCT+TGG | 0.419007 | 3.1:-68926010 | None:intergenic |
| TGATGAACTCTTGGAAATCA+TGG | 0.438584 | 3.1:-68926001 | None:intergenic |
| AAGGACAGTGCAAGAGATGA+TGG | 0.443408 | 3.1:+68926185 | MS.gene066802:CDS |
| TCTCCTTCTTTAATCTCCCA+TGG | 0.457000 | 3.1:-68926318 | None:intergenic |
| TCATCGCCGCTACCGATTGA+AGG | 0.462817 | 3.1:+68926264 | MS.gene066802:CDS |
| CTCTCTTCTTGGGTTCGGAC+TGG | 0.469722 | 3.1:-68926116 | None:intergenic |
| TCAATTGTACTCATTGCAAA+CGG | 0.479546 | 3.1:-68926234 | None:intergenic |
| CATAACCCTATCGGTGCAGC+AGG | 0.490168 | 3.1:-68926144 | None:intergenic |
| CAATTGTACTCATTGCAAAC+GGG | 0.496144 | 3.1:-68926233 | None:intergenic |
| TCAATCGGTAGCGGCGATGA+AGG | 0.505690 | 3.1:-68926261 | None:intergenic |
| CTTCGAGTCAAAGCCATGAC+AGG | 0.510747 | 3.1:+68926039 | MS.gene066802:CDS |
| AGTAGGTGGGTATAGAAGGA+GGG | 0.522121 | 3.1:+68926287 | MS.gene066802:CDS |
| ACATCCTCTTTGTTCATACC+AGG | 0.528873 | 3.1:-68926369 | None:intergenic |
| GAGTAGGTGGGTATAGAAGG+AGG | 0.533601 | 3.1:+68926286 | MS.gene066802:CDS |
| CTGTCATGGCTTTGACTCGA+AGG | 0.534564 | 3.1:-68926038 | None:intergenic |
| TACCCACCTACTCCTTCAAT+CGG | 0.537111 | 3.1:-68926276 | None:intergenic |
| AACTCTTGGAAATCATGGAC+AGG | 0.539369 | 3.1:-68925996 | None:intergenic |
| ACACCATGGGAGATTAAAGA+AGG | 0.546168 | 3.1:+68926315 | MS.gene066802:CDS |
| GTAGGTGGGTATAGAAGGAG+GGG | 0.548522 | 3.1:+68926288 | MS.gene066802:CDS |
| GAGAAAGTGAATACAAGATG+AGG | 0.559520 | 3.1:+68926337 | MS.gene066802:CDS |
| AAGATGAGGTTTGATATGCC+TGG | 0.574216 | 3.1:+68926351 | MS.gene066802:CDS |
| CCGCTACCGATTGAAGGAGT+AGG | 0.578817 | 3.1:+68926270 | MS.gene066802:CDS |
| TCTTGCACTGTCCTTGTCGT+AGG | 0.581285 | 3.1:-68926177 | None:intergenic |
| GGGAACAATGGAAAGCATGA+TGG | 0.589950 | 3.1:+68926206 | MS.gene066802:CDS |
| TCTTCTTGGGTTCGGACTGG+CGG | 0.591487 | 3.1:-68926113 | None:intergenic |
| GCAAGAGATGATGGGAACAA+TGG | 0.598164 | 3.1:+68926194 | MS.gene066802:CDS |
| AATCGATCCCATAACCCTAT+CGG | 0.598439 | 3.1:-68926153 | None:intergenic |
| AGGACAGTGCAAGAGATGAT+GGG | 0.601662 | 3.1:+68926186 | MS.gene066802:CDS |
| TATTGTTTGATCTTGAAACA+TGG | 0.608054 | 3.1:-68926083 | None:intergenic |
| TTGCAATGAGTACAATTGAG+TGG | 0.632366 | 3.1:+68926238 | MS.gene066802:CDS |
| CTACCGATTGAAGGAGTAGG+TGG | 0.639864 | 3.1:+68926273 | MS.gene066802:CDS |
| AAGGAGGGGAAGAACACCAT+GGG | 0.642482 | 3.1:+68926302 | MS.gene066802:CDS |
| CCTACTCCTTCAATCGGTAG+CGG | 0.682306 | 3.1:-68926270 | None:intergenic |
| TACCGATTGAAGGAGTAGGT+GGG | 0.683545 | 3.1:+68926274 | MS.gene066802:CDS |
| GAAGGAGGGGAAGAACACCA+TGG | 0.687857 | 3.1:+68926301 | MS.gene066802:CDS |
| GGGATCGATTTCCTACGACA+AGG | 0.701576 | 3.1:+68926166 | MS.gene066802:CDS |
| TATGCCTGGTATGAACAAAG+AGG | 0.732695 | 3.1:+68926365 | MS.gene066802:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATTGTTTGATCTTGAAACA+TGG | - | chr3.1:68926086-68926105 | None:intergenic | 25.0% |
| ACAAAGAGGATGTTAAAGTT+TGG | + | chr3.1:68926379-68926398 | MS.gene066802:CDS | 30.0% | |
| CACTCTTTATCCTTAAATCT+TGG | + | chr3.1:68925967-68925986 | MS.gene066802:CDS | 30.0% | |
| TCAATTGTACTCATTGCAAA+CGG | - | chr3.1:68926237-68926256 | None:intergenic | 30.0% | |
| AAAAACTCTCTTCTTGGGTT+CGG | - | chr3.1:68926124-68926143 | None:intergenic | 35.0% | |
| AGTTTGTCTTGATGAACTCT+TGG | - | chr3.1:68926013-68926032 | None:intergenic | 35.0% | |
| CAATTGTACTCATTGCAAAC+GGG | - | chr3.1:68926236-68926255 | None:intergenic | 35.0% | |
| CAGGTTAAGACCAAGATTTA+AGG | - | chr3.1:68925980-68925999 | None:intergenic | 35.0% | |
| GAGAAAGTGAATACAAGATG+AGG | + | chr3.1:68926337-68926356 | MS.gene066802:CDS | 35.0% | |
| TGATGAACTCTTGGAAATCA+TGG | - | chr3.1:68926004-68926023 | None:intergenic | 35.0% | |
| TTGCAATGAGTACAATTGAG+TGG | + | chr3.1:68926238-68926257 | MS.gene066802:CDS | 35.0% | |
| AACTCTTGGAAATCATGGAC+AGG | - | chr3.1:68925999-68926018 | None:intergenic | 40.0% | |
| AAGATGAGGTTTGATATGCC+TGG | + | chr3.1:68926351-68926370 | MS.gene066802:CDS | 40.0% | |
| AATCGATCCCATAACCCTAT+CGG | - | chr3.1:68926156-68926175 | None:intergenic | 40.0% | |
| ACACCATGGGAGATTAAAGA+AGG | + | chr3.1:68926315-68926334 | MS.gene066802:CDS | 40.0% | |
| ACATCCTCTTTGTTCATACC+AGG | - | chr3.1:68926372-68926391 | None:intergenic | 40.0% | |
| AGCAGGAAAAACTCTCTTCT+TGG | - | chr3.1:68926130-68926149 | None:intergenic | 40.0% | |
| GCAGGAAAAACTCTCTTCTT+GGG | - | chr3.1:68926129-68926148 | None:intergenic | 40.0% | |
| TATGCCTGGTATGAACAAAG+AGG | + | chr3.1:68926365-68926384 | MS.gene066802:CDS | 40.0% | |
| TCTCCTTCTTTAATCTCCCA+TGG | - | chr3.1:68926321-68926340 | None:intergenic | 40.0% | |
| AAGGACAGTGCAAGAGATGA+TGG | + | chr3.1:68926185-68926204 | MS.gene066802:CDS | 45.0% | |
| AAGGAGTAGGTGGGTATAGA+AGG | + | chr3.1:68926283-68926302 | MS.gene066802:CDS | 45.0% | |
| AGGACAGTGCAAGAGATGAT+GGG | + | chr3.1:68926186-68926205 | MS.gene066802:CDS | 45.0% | |
| AGTAGGTGGGTATAGAAGGA+GGG | + | chr3.1:68926287-68926306 | MS.gene066802:CDS | 45.0% | |
| TACCCACCTACTCCTTCAAT+CGG | - | chr3.1:68926279-68926298 | None:intergenic | 45.0% | |
| TTCTCTTGCTTCACCTGTCA+TGG | - | chr3.1:68926055-68926074 | None:intergenic | 45.0% | |
| ! | GGGAACAATGGAAAGCATGA+TGG | + | chr3.1:68926206-68926225 | MS.gene066802:CDS | 45.0% |
| ! | TACCGATTGAAGGAGTAGGT+GGG | + | chr3.1:68926274-68926293 | MS.gene066802:CDS | 45.0% |
| !! | GCAAGAGATGATGGGAACAA+TGG | + | chr3.1:68926194-68926213 | MS.gene066802:CDS | 45.0% |
| !! | TTTTTCCTGCTGCACCGATA+GGG | + | chr3.1:68926139-68926158 | MS.gene066802:CDS | 45.0% |
| AAGGAGGGGAAGAACACCAT+GGG | + | chr3.1:68926302-68926321 | MS.gene066802:CDS | 50.0% | |
| CCTACTCCTTCAATCGGTAG+CGG | - | chr3.1:68926273-68926292 | None:intergenic | 50.0% | |
| CTTCGAGTCAAAGCCATGAC+AGG | + | chr3.1:68926039-68926058 | MS.gene066802:CDS | 50.0% | |
| GAGTAGGTGGGTATAGAAGG+AGG | + | chr3.1:68926286-68926305 | MS.gene066802:CDS | 50.0% | |
| GTAGGTGGGTATAGAAGGAG+GGG | + | chr3.1:68926288-68926307 | MS.gene066802:CDS | 50.0% | |
| TCTTGCACTGTCCTTGTCGT+AGG | - | chr3.1:68926180-68926199 | None:intergenic | 50.0% | |
| ! | CTACCGATTGAAGGAGTAGG+TGG | + | chr3.1:68926273-68926292 | MS.gene066802:CDS | 50.0% |
| ! | GGGATCGATTTCCTACGACA+AGG | + | chr3.1:68926166-68926185 | MS.gene066802:CDS | 50.0% |
| ! | TGCTGCACCGATAGGGTTAT+GGG | + | chr3.1:68926146-68926165 | MS.gene066802:CDS | 50.0% |
| !! | CTGTCATGGCTTTGACTCGA+AGG | - | chr3.1:68926041-68926060 | None:intergenic | 50.0% |
| !! | GTTTTTCCTGCTGCACCGAT+AGG | + | chr3.1:68926138-68926157 | MS.gene066802:CDS | 50.0% |
| CATAACCCTATCGGTGCAGC+AGG | - | chr3.1:68926147-68926166 | None:intergenic | 55.0% | |
| CTCTCTTCTTGGGTTCGGAC+TGG | - | chr3.1:68926119-68926138 | None:intergenic | 55.0% | |
| GAAGGAGGGGAAGAACACCA+TGG | + | chr3.1:68926301-68926320 | MS.gene066802:CDS | 55.0% | |
| ! | CCGCTACCGATTGAAGGAGT+AGG | + | chr3.1:68926270-68926289 | MS.gene066802:CDS | 55.0% |
| ! | CTGCTGCACCGATAGGGTTA+TGG | + | chr3.1:68926145-68926164 | MS.gene066802:CDS | 55.0% |
| ! | TCAATCGGTAGCGGCGATGA+AGG | - | chr3.1:68926264-68926283 | None:intergenic | 55.0% |
| ! | TCATCGCCGCTACCGATTGA+AGG | + | chr3.1:68926264-68926283 | MS.gene066802:CDS | 55.0% |
| ! | TCTTCTTGGGTTCGGACTGG+CGG | - | chr3.1:68926116-68926135 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 68925961 | 68926401 | 68925961 | ID=MS.gene066802 |
| chr3.1 | mRNA | 68925961 | 68926401 | 68925961 | ID=MS.gene066802.t1;Parent=MS.gene066802 |
| chr3.1 | exon | 68925961 | 68926401 | 68925961 | ID=MS.gene066802.t1.exon1;Parent=MS.gene066802.t1 |
| chr3.1 | CDS | 68925961 | 68926401 | 68925961 | ID=cds.MS.gene066802.t1;Parent=MS.gene066802.t1 |
| Gene Sequence |
| Protein sequence |
