Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24521.t1 | XP_013465937.1 | 95.3 | 295 | 13 | 1 | 1 | 294 | 47 | 341 | 1.10E-136 | 496.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24521.t1 | Q9M2Y9 | 94.2 | 69 | 4 | 0 | 1 | 69 | 47 | 115 | 6.1e-35 | 149.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24521.t1 | A0A072VEW3 | 95.3 | 295 | 13 | 1 | 1 | 294 | 47 | 341 | 8.0e-137 | 496.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene24521 | -0.862291 | 5.62E-64 | -1.69E-46 |
| MS.gene049222 | MS.gene24521 | -0.844402 | 7.73E-59 | -1.69E-46 |
| MS.gene049232 | MS.gene24521 | -0.825484 | 4.57E-54 | -1.69E-46 |
| MS.gene049285 | MS.gene24521 | -0.828221 | 1.01E-54 | -1.69E-46 |
| MS.gene049320 | MS.gene24521 | -0.831495 | 1.62E-55 | -1.69E-46 |
| MS.gene04932 | MS.gene24521 | 0.816497 | 5.37E-52 | -1.69E-46 |
| MS.gene049609 | MS.gene24521 | -0.807361 | 5.25E-50 | -1.69E-46 |
| MS.gene049611 | MS.gene24521 | 0.819371 | 1.20E-52 | -1.69E-46 |
| MS.gene049633 | MS.gene24521 | -0.851199 | 1.04E-60 | -1.69E-46 |
| MS.gene049634 | MS.gene24521 | 0.809182 | 2.15E-50 | -1.69E-46 |
| MS.gene050082 | MS.gene24521 | 0.812417 | 4.28E-51 | -1.69E-46 |
| MS.gene05010 | MS.gene24521 | -0.824734 | 6.87E-54 | -1.69E-46 |
| MS.gene050165 | MS.gene24521 | -0.800004 | 1.77E-48 | -1.69E-46 |
| MS.gene050170 | MS.gene24521 | -0.878449 | 2.78E-69 | -1.69E-46 |
| MS.gene050216 | MS.gene24521 | -0.800731 | 1.26E-48 | -1.69E-46 |
| MS.gene050323 | MS.gene24521 | -0.880234 | 6.49E-70 | -1.69E-46 |
| MS.gene050333 | MS.gene24521 | -0.802457 | 5.56E-49 | -1.69E-46 |
| MS.gene050338 | MS.gene24521 | 0.825589 | 4.32E-54 | -1.69E-46 |
| MS.gene050381 | MS.gene24521 | 0.82997 | 3.82E-55 | -1.69E-46 |
| MS.gene050444 | MS.gene24521 | -0.837399 | 5.32E-57 | -1.69E-46 |
| MS.gene050496 | MS.gene24521 | -0.868051 | 8.70E-66 | -1.69E-46 |
| MS.gene050504 | MS.gene24521 | -0.859676 | 3.51E-63 | -1.69E-46 |
| MS.gene050518 | MS.gene24521 | -0.8178 | 2.73E-52 | -1.69E-46 |
| MS.gene050577 | MS.gene24521 | -0.84319 | 1.63E-58 | -1.69E-46 |
| MS.gene050612 | MS.gene24521 | -0.868514 | 6.17E-66 | -1.69E-46 |
| MS.gene050675 | MS.gene24521 | -0.858953 | 5.78E-63 | -1.69E-46 |
| MS.gene050697 | MS.gene24521 | -0.819744 | 9.89E-53 | -1.69E-46 |
| MS.gene050699 | MS.gene24521 | -0.829859 | 4.07E-55 | -1.69E-46 |
| MS.gene050747 | MS.gene24521 | -0.812373 | 4.38E-51 | -1.69E-46 |
| MS.gene050772 | MS.gene24521 | -0.812822 | 3.49E-51 | -1.69E-46 |
| MS.gene050809 | MS.gene24521 | 0.816112 | 6.54E-52 | -1.69E-46 |
| MS.gene05080 | MS.gene24521 | -0.859654 | 3.56E-63 | -1.69E-46 |
| MS.gene05081 | MS.gene24521 | -0.811819 | 5.79E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene24521 | -0.881836 | 1.72E-70 | -1.69E-46 |
| MS.gene050871 | MS.gene24521 | -0.877599 | 5.52E-69 | -1.69E-46 |
| MS.gene050904 | MS.gene24521 | -0.834886 | 2.31E-56 | -1.69E-46 |
| MS.gene050950 | MS.gene24521 | -0.807192 | 5.70E-50 | -1.69E-46 |
| MS.gene050986 | MS.gene24521 | -0.83767 | 4.53E-57 | -1.69E-46 |
| MS.gene051044 | MS.gene24521 | 0.819427 | 1.17E-52 | -1.69E-46 |
| MS.gene05104 | MS.gene24521 | 0.831585 | 1.54E-55 | -1.69E-46 |
| MS.gene051127 | MS.gene24521 | 0.82197 | 3.04E-53 | -1.69E-46 |
| MS.gene051138 | MS.gene24521 | 0.87571 | 2.49E-68 | -1.69E-46 |
| MS.gene051268 | MS.gene24521 | -0.856111 | 4.01E-62 | -1.69E-46 |
| MS.gene051317 | MS.gene24521 | -0.852145 | 5.59E-61 | -1.69E-46 |
| MS.gene051364 | MS.gene24521 | 0.806589 | 7.64E-50 | -1.69E-46 |
| MS.gene051366 | MS.gene24521 | 0.807802 | 4.23E-50 | -1.69E-46 |
| MS.gene051439 | MS.gene24521 | 0.811558 | 6.59E-51 | -1.69E-46 |
| MS.gene051440 | MS.gene24521 | 0.802962 | 4.37E-49 | -1.69E-46 |
| MS.gene051651 | MS.gene24521 | 0.874897 | 4.71E-68 | -1.69E-46 |
| MS.gene051749 | MS.gene24521 | 0.82015 | 7.99E-53 | -1.69E-46 |
| MS.gene051838 | MS.gene24521 | -0.843876 | 1.07E-58 | -1.69E-46 |
| MS.gene051874 | MS.gene24521 | -0.827515 | 1.50E-54 | -1.69E-46 |
| MS.gene052129 | MS.gene24521 | 0.843809 | 1.12E-58 | -1.69E-46 |
| MS.gene052164 | MS.gene24521 | -0.841529 | 4.50E-58 | -1.69E-46 |
| MS.gene052211 | MS.gene24521 | 0.810062 | 1.39E-50 | -1.69E-46 |
| MS.gene05240 | MS.gene24521 | 0.807834 | 4.17E-50 | -1.69E-46 |
| MS.gene052482 | MS.gene24521 | -0.820688 | 6.01E-53 | -1.69E-46 |
| MS.gene052486 | MS.gene24521 | -0.815975 | 7.02E-52 | -1.69E-46 |
| MS.gene052494 | MS.gene24521 | 0.803224 | 3.86E-49 | -1.69E-46 |
| MS.gene052503 | MS.gene24521 | -0.824207 | 9.15E-54 | -1.69E-46 |
| MS.gene052506 | MS.gene24521 | -0.806525 | 7.88E-50 | -1.69E-46 |
| MS.gene052606 | MS.gene24521 | 0.852185 | 5.45E-61 | -1.69E-46 |
| MS.gene052615 | MS.gene24521 | -0.801629 | 8.23E-49 | -1.69E-46 |
| MS.gene052822 | MS.gene24521 | -0.809149 | 2.18E-50 | -1.69E-46 |
| MS.gene052823 | MS.gene24521 | -0.830351 | 3.08E-55 | -1.69E-46 |
| MS.gene052825 | MS.gene24521 | -0.830148 | 3.46E-55 | -1.69E-46 |
| MS.gene052872 | MS.gene24521 | -0.811705 | 6.13E-51 | -1.69E-46 |
| MS.gene052966 | MS.gene24521 | -0.85657 | 2.94E-62 | -1.69E-46 |
| MS.gene053097 | MS.gene24521 | 0.819748 | 9.87E-53 | -1.69E-46 |
| MS.gene053203 | MS.gene24521 | -0.864013 | 1.65E-64 | -1.69E-46 |
| MS.gene053420 | MS.gene24521 | 0.843041 | 1.79E-58 | -1.69E-46 |
| MS.gene053421 | MS.gene24521 | 0.801269 | 9.75E-49 | -1.69E-46 |
| MS.gene053436 | MS.gene24521 | -0.800357 | 1.50E-48 | -1.69E-46 |
| MS.gene053479 | MS.gene24521 | -0.845085 | 5.06E-59 | -1.69E-46 |
| MS.gene053480 | MS.gene24521 | -0.810276 | 1.25E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene24521 | -0.807003 | 6.25E-50 | -1.69E-46 |
| MS.gene053632 | MS.gene24521 | -0.810691 | 1.02E-50 | -1.69E-46 |
| MS.gene053776 | MS.gene24521 | 0.803503 | 3.38E-49 | -1.69E-46 |
| MS.gene053824 | MS.gene24521 | -0.84459 | 6.88E-59 | -1.69E-46 |
| MS.gene053887 | MS.gene24521 | 0.8268 | 2.22E-54 | -1.69E-46 |
| MS.gene053916 | MS.gene24521 | -0.827861 | 1.24E-54 | -1.69E-46 |
| MS.gene054344 | MS.gene24521 | -0.804829 | 1.79E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene24521 | -0.834504 | 2.88E-56 | -1.69E-46 |
| MS.gene054642 | MS.gene24521 | -0.814322 | 1.63E-51 | -1.69E-46 |
| MS.gene054706 | MS.gene24521 | 0.86181 | 7.90E-64 | -1.69E-46 |
| MS.gene054707 | MS.gene24521 | 0.82494 | 6.15E-54 | -1.69E-46 |
| MS.gene05470 | MS.gene24521 | -0.856002 | 4.32E-62 | -1.69E-46 |
| MS.gene054742 | MS.gene24521 | 0.803862 | 2.85E-49 | -1.69E-46 |
| MS.gene05494 | MS.gene24521 | -0.825231 | 5.25E-54 | -1.69E-46 |
| MS.gene054954 | MS.gene24521 | -0.843203 | 1.62E-58 | -1.69E-46 |
| MS.gene055154 | MS.gene24521 | 0.800673 | 1.29E-48 | -1.69E-46 |
| MS.gene055158 | MS.gene24521 | 0.841872 | 3.65E-58 | -1.69E-46 |
| MS.gene055202 | MS.gene24521 | 0.809032 | 2.31E-50 | -1.69E-46 |
| MS.gene055206 | MS.gene24521 | -0.806807 | 6.87E-50 | -1.69E-46 |
| MS.gene055362 | MS.gene24521 | -0.83468 | 2.61E-56 | -1.69E-46 |
| MS.gene055502 | MS.gene24521 | -0.864591 | 1.09E-64 | -1.69E-46 |
| MS.gene055577 | MS.gene24521 | -0.866557 | 2.61E-65 | -1.69E-46 |
| MS.gene055748 | MS.gene24521 | -0.859824 | 3.17E-63 | -1.69E-46 |
| MS.gene055750 | MS.gene24521 | -0.845788 | 3.26E-59 | -1.69E-46 |
| MS.gene055751 | MS.gene24521 | -0.862256 | 5.76E-64 | -1.69E-46 |
| MS.gene055776 | MS.gene24521 | -0.822965 | 1.79E-53 | -1.69E-46 |
| MS.gene05589 | MS.gene24521 | -0.890838 | 6.86E-74 | -1.69E-46 |
| MS.gene055941 | MS.gene24521 | -0.813112 | 3.02E-51 | -1.69E-46 |
| MS.gene055942 | MS.gene24521 | 0.815282 | 1.00E-51 | -1.69E-46 |
| MS.gene056198 | MS.gene24521 | 0.839747 | 1.32E-57 | -1.69E-46 |
| MS.gene056218 | MS.gene24521 | 0.802031 | 6.80E-49 | -1.69E-46 |
| MS.gene056224 | MS.gene24521 | 0.833892 | 4.11E-56 | -1.69E-46 |
| MS.gene056359 | MS.gene24521 | 0.802899 | 4.51E-49 | -1.69E-46 |
| MS.gene056387 | MS.gene24521 | -0.870294 | 1.63E-66 | -1.69E-46 |
| MS.gene056504 | MS.gene24521 | -0.891182 | 5.02E-74 | -1.69E-46 |
| MS.gene056510 | MS.gene24521 | -0.849658 | 2.81E-60 | -1.69E-46 |
| MS.gene056590 | MS.gene24521 | 0.812888 | 3.38E-51 | -1.69E-46 |
| MS.gene056591 | MS.gene24521 | 0.812415 | 4.29E-51 | -1.69E-46 |
| MS.gene056618 | MS.gene24521 | 0.802762 | 4.81E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene24521 | -0.825862 | 3.72E-54 | -1.69E-46 |
| MS.gene056678 | MS.gene24521 | 0.817083 | 3.96E-52 | -1.69E-46 |
| MS.gene056688 | MS.gene24521 | 0.818403 | 2.00E-52 | -1.69E-46 |
| MS.gene056692 | MS.gene24521 | -0.820081 | 8.28E-53 | -1.69E-46 |
| MS.gene056694 | MS.gene24521 | 0.835777 | 1.38E-56 | -1.69E-46 |
| MS.gene056697 | MS.gene24521 | 0.808402 | 3.15E-50 | -1.69E-46 |
| MS.gene056748 | MS.gene24521 | 0.830649 | 2.61E-55 | -1.69E-46 |
| MS.gene056856 | MS.gene24521 | 0.851958 | 6.32E-61 | -1.69E-46 |
| MS.gene056857 | MS.gene24521 | 0.80815 | 3.57E-50 | -1.69E-46 |
| MS.gene057014 | MS.gene24521 | 0.806911 | 6.54E-50 | -1.69E-46 |
| MS.gene057086 | MS.gene24521 | -0.831372 | 1.73E-55 | -1.69E-46 |
| MS.gene05753 | MS.gene24521 | -0.807532 | 4.83E-50 | -1.69E-46 |
| MS.gene057544 | MS.gene24521 | 0.825582 | 4.33E-54 | -1.69E-46 |
| MS.gene057658 | MS.gene24521 | -0.816857 | 4.45E-52 | -1.69E-46 |
| MS.gene057717 | MS.gene24521 | 0.802862 | 4.59E-49 | -1.69E-46 |
| MS.gene057768 | MS.gene24521 | 0.856433 | 3.23E-62 | -1.69E-46 |
| MS.gene057831 | MS.gene24521 | -0.807224 | 5.61E-50 | -1.69E-46 |
| MS.gene057857 | MS.gene24521 | -0.802386 | 5.75E-49 | -1.69E-46 |
| MS.gene05787 | MS.gene24521 | -0.801856 | 7.39E-49 | -1.69E-46 |
| MS.gene058068 | MS.gene24521 | 0.801379 | 9.26E-49 | -1.69E-46 |
| MS.gene058093 | MS.gene24521 | 0.804024 | 2.63E-49 | -1.69E-46 |
| MS.gene058099 | MS.gene24521 | 0.828931 | 6.83E-55 | -1.69E-46 |
| MS.gene058109 | MS.gene24521 | 0.809026 | 2.32E-50 | -1.69E-46 |
| MS.gene058157 | MS.gene24521 | -0.82118 | 4.63E-53 | -1.69E-46 |
| MS.gene058171 | MS.gene24521 | 0.84354 | 1.32E-58 | -1.69E-46 |
| MS.gene058173 | MS.gene24521 | 0.839402 | 1.62E-57 | -1.69E-46 |
| MS.gene058175 | MS.gene24521 | 0.852042 | 5.98E-61 | -1.69E-46 |
| MS.gene058268 | MS.gene24521 | 0.805052 | 1.61E-49 | -1.69E-46 |
| MS.gene058334 | MS.gene24521 | 0.857935 | 1.16E-62 | -1.69E-46 |
| MS.gene058338 | MS.gene24521 | -0.808835 | 2.55E-50 | -1.69E-46 |
| MS.gene05837 | MS.gene24521 | -0.8452 | 4.71E-59 | -1.69E-46 |
| MS.gene058382 | MS.gene24521 | 0.834284 | 3.28E-56 | -1.69E-46 |
| MS.gene058418 | MS.gene24521 | 0.826784 | 2.24E-54 | -1.69E-46 |
| MS.gene058419 | MS.gene24521 | -0.831315 | 1.79E-55 | -1.69E-46 |
| MS.gene058452 | MS.gene24521 | 0.810187 | 1.31E-50 | -1.69E-46 |
| MS.gene058544 | MS.gene24521 | -0.831469 | 1.64E-55 | -1.69E-46 |
| MS.gene058603 | MS.gene24521 | 0.839494 | 1.53E-57 | -1.69E-46 |
| MS.gene058773 | MS.gene24521 | -0.82635 | 2.85E-54 | -1.69E-46 |
| MS.gene058838 | MS.gene24521 | -0.864117 | 1.53E-64 | -1.69E-46 |
| MS.gene059022 | MS.gene24521 | -0.849876 | 2.44E-60 | -1.69E-46 |
| MS.gene059023 | MS.gene24521 | -0.847723 | 9.66E-60 | -1.69E-46 |
| MS.gene059045 | MS.gene24521 | 0.82753 | 1.49E-54 | -1.69E-46 |
| MS.gene05913 | MS.gene24521 | -0.813545 | 2.43E-51 | -1.69E-46 |
| MS.gene05914 | MS.gene24521 | -0.833576 | 4.93E-56 | -1.69E-46 |
| MS.gene05926 | MS.gene24521 | 0.83273 | 8.00E-56 | -1.69E-46 |
| MS.gene059315 | MS.gene24521 | 0.8054 | 1.36E-49 | -1.69E-46 |
| MS.gene059367 | MS.gene24521 | -0.815818 | 7.61E-52 | -1.69E-46 |
| MS.gene059526 | MS.gene24521 | 0.80868 | 2.75E-50 | -1.69E-46 |
| MS.gene05960 | MS.gene24521 | 0.818637 | 1.77E-52 | -1.69E-46 |
| MS.gene059667 | MS.gene24521 | -0.843952 | 1.02E-58 | -1.69E-46 |
| MS.gene059677 | MS.gene24521 | -0.838246 | 3.22E-57 | -1.69E-46 |
| MS.gene059699 | MS.gene24521 | 0.832453 | 9.37E-56 | -1.69E-46 |
| MS.gene059701 | MS.gene24521 | 0.802911 | 4.48E-49 | -1.69E-46 |
| MS.gene059703 | MS.gene24521 | 0.845549 | 3.79E-59 | -1.69E-46 |
| MS.gene059718 | MS.gene24521 | -0.834387 | 3.09E-56 | -1.69E-46 |
| MS.gene059865 | MS.gene24521 | -0.87809 | 3.72E-69 | -1.69E-46 |
| MS.gene06000 | MS.gene24521 | -0.864824 | 9.21E-65 | -1.69E-46 |
| MS.gene06027 | MS.gene24521 | -0.848183 | 7.21E-60 | -1.69E-46 |
| MS.gene060405 | MS.gene24521 | 0.827676 | 1.37E-54 | -1.69E-46 |
| MS.gene060593 | MS.gene24521 | 0.824119 | 9.59E-54 | -1.69E-46 |
| MS.gene060595 | MS.gene24521 | 0.832256 | 1.05E-55 | -1.69E-46 |
| MS.gene060762 | MS.gene24521 | -0.865732 | 4.77E-65 | -1.69E-46 |
| MS.gene060778 | MS.gene24521 | -0.861102 | 1.30E-63 | -1.69E-46 |
| MS.gene06077 | MS.gene24521 | -0.847875 | 8.77E-60 | -1.69E-46 |
| MS.gene060823 | MS.gene24521 | 0.827151 | 1.83E-54 | -1.69E-46 |
| MS.gene06084 | MS.gene24521 | 0.802529 | 5.37E-49 | -1.69E-46 |
| MS.gene06097 | MS.gene24521 | -0.800051 | 1.73E-48 | -1.69E-46 |
| MS.gene060982 | MS.gene24521 | -0.868101 | 8.39E-66 | -1.69E-46 |
| MS.gene061044 | MS.gene24521 | 0.862284 | 5.65E-64 | -1.69E-46 |
| MS.gene061150 | MS.gene24521 | -0.807017 | 6.21E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 56 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAATTTAACCTTGGTATT+TGG | 0.131923 | 1.1:+5331431 | None:intergenic |
| TACAAGGTTCTAGTATTAAT+TGG | 0.175164 | 1.1:-5331494 | MS.gene24521:CDS |
| CTTGATCATTGAAGCCTTGT+TGG | 0.313961 | 1.1:+5331871 | None:intergenic |
| AACCACCATGTTTGAGATTA+GGG | 0.322373 | 1.1:+5332527 | None:intergenic |
| CAACCATCAAAAGTAGTATT+AGG | 0.326180 | 1.1:+5331792 | None:intergenic |
| CTTGTTGCTGCTGATCTTTA+CGG | 0.335855 | 1.1:+5332075 | None:intergenic |
| ACAAGGTTCTAGTATTAATT+GGG | 0.336603 | 1.1:-5331493 | MS.gene24521:CDS |
| AAACCACCATGTTTGAGATT+AGG | 0.366318 | 1.1:+5332526 | None:intergenic |
| AATTATCCACCAAGATTTGA+TGG | 0.368986 | 1.1:-5331567 | MS.gene24521:CDS |
| CATTCCAGAGATTGATCATA+TGG | 0.369915 | 1.1:-5331598 | MS.gene24521:CDS |
| ATAATGACATAAAGAACTAT+TGG | 0.370430 | 1.1:-5332133 | MS.gene24521:CDS |
| TTGGTTGTGGTTGTTATCAT+TGG | 0.385791 | 1.1:+5331680 | None:intergenic |
| GATAATGTTATCTTCTTCTT+CGG | 0.391795 | 1.1:+5332501 | None:intergenic |
| ATGTCATTATCAGTTCTTCC+TGG | 0.397545 | 1.1:+5332146 | None:intergenic |
| TTCTTGAACCAAATTTGATA+AGG | 0.407035 | 1.1:+5331974 | None:intergenic |
| TGCATGACATGTGGTAAATA+TGG | 0.407502 | 1.1:+5331948 | None:intergenic |
| AGAAAACTTCTTATGAAGCT+TGG | 0.411643 | 1.1:-5331840 | MS.gene24521:CDS |
| TGCAGCCTTTACATTAGTAT+TGG | 0.421799 | 1.1:-5332478 | MS.gene24521:CDS |
| GCTCGTAATAGAGGCAATAA+TGG | 0.432154 | 1.1:-5332053 | MS.gene24521:CDS |
| CAGCAACAAGCTCGTAATAG+AGG | 0.461617 | 1.1:-5332062 | MS.gene24521:CDS |
| AATTCCAACCCATCAAATCT+TGG | 0.462721 | 1.1:+5331558 | None:intergenic |
| TCCACCAAGATTTGATGGGT+TGG | 0.464644 | 1.1:-5331562 | MS.gene24521:CDS |
| CATTTGTTGCGAATTGTACT+TGG | 0.466808 | 1.1:+5331661 | None:intergenic |
| AACATTGATGGTAGTGGAGG+TGG | 0.467582 | 1.1:+5331906 | None:intergenic |
| CAAGTACAATTCGCAACAAA+TGG | 0.468082 | 1.1:-5331660 | MS.gene24521:CDS |
| TTGCGAATTGTACTTGGTTG+TGG | 0.482944 | 1.1:+5331667 | None:intergenic |
| AAACTTCTTATGAAGCTTGG+AGG | 0.489488 | 1.1:-5331837 | MS.gene24521:CDS |
| TTTACATTAGTATTGGAAGC+AGG | 0.496580 | 1.1:-5332471 | MS.gene24521:intron |
| GATACCATATGATCAATCTC+TGG | 0.503391 | 1.1:+5331594 | None:intergenic |
| TTTGTGTATGACAACATTGA+TGG | 0.508144 | 1.1:+5331894 | None:intergenic |
| TGCTTCCAATACTAATGTAA+AGG | 0.512738 | 1.1:+5332473 | None:intergenic |
| GTGGAAAGAGTTGTCGTCTC+CGG | 0.522056 | 1.1:-5332567 | MS.gene24521:CDS |
| CACAAATCACAATTTAACCT+TGG | 0.527729 | 1.1:+5331424 | None:intergenic |
| CATTGATGGTAGTGGAGGTG+GGG | 0.533607 | 1.1:+5331908 | None:intergenic |
| ACATTGATGGTAGTGGAGGT+GGG | 0.534119 | 1.1:+5331907 | None:intergenic |
| ATAATTGCAGCTCAATTGCC+AGG | 0.552173 | 1.1:-5332164 | MS.gene24521:CDS |
| ATTATCCACCAAGATTTGAT+GGG | 0.556403 | 1.1:-5331566 | MS.gene24521:CDS |
| TATGACAACATTGATGGTAG+TGG | 0.558740 | 1.1:+5331900 | None:intergenic |
| ATTGATGGTAGTGGAGGTGG+GGG | 0.560501 | 1.1:+5331909 | None:intergenic |
| GGCGAAGATAGTTTAGCCAC+CGG | 0.569935 | 1.1:+5332548 | None:intergenic |
| TAAAGAACTATTGGAATACA+AGG | 0.580554 | 1.1:-5332124 | MS.gene24521:CDS |
| AAGATGATGATAACTTGAAG+TGG | 0.584448 | 1.1:+5331461 | None:intergenic |
| CTTCTTGATTCCAAATACCA+AGG | 0.584605 | 1.1:-5331441 | MS.gene24521:CDS |
| TCCAACCCATCAAATCTTGG+TGG | 0.591459 | 1.1:+5331561 | None:intergenic |
| ATTGATAATAATAAGATACA+AGG | 0.601954 | 1.1:-5331510 | MS.gene24521:CDS |
| AGTTTATGATCAAGAACATG+TGG | 0.602665 | 1.1:-5331727 | MS.gene24521:CDS |
| GAAAGAGTTGTCGTCTCCGG+TGG | 0.603936 | 1.1:-5332564 | MS.gene24521:CDS |
| CTTCGCCCTAATCTCAAACA+TGG | 0.609085 | 1.1:-5332532 | MS.gene24521:CDS |
| GACTCGGTGCAAGAAGAGCA+AGG | 0.613143 | 1.1:-5331627 | MS.gene24521:CDS |
| CAAGGTTCTAGTATTAATTG+GGG | 0.621220 | 1.1:-5331492 | MS.gene24521:CDS |
| CGCCCTAATCTCAAACATGG+TGG | 0.637157 | 1.1:-5332529 | MS.gene24521:CDS |
| TTGTCATACACAAACCAACA+AGG | 0.660089 | 1.1:-5331885 | MS.gene24521:CDS |
| GTTTATGATCAAGAACATGT+GGG | 0.665568 | 1.1:-5331726 | MS.gene24521:CDS |
| AAATGGTCAATACTTTGACT+CGG | 0.679619 | 1.1:-5331643 | MS.gene24521:CDS |
| GACAACATTGATGGTAGTGG+AGG | 0.681316 | 1.1:+5331903 | None:intergenic |
| AGCAAAGGTTGCATGACATG+TGG | 0.714384 | 1.1:+5331939 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAAAAAAATTCAAACTAT+TGG | + | chr1.1:5331743-5331762 | None:intergenic | 10.0% |
| !!! | ATTGATAATAATAAGATACA+AGG | - | chr1.1:5332506-5332525 | MS.gene24521:CDS | 15.0% |
| !! | ACAAAAAGTAACATATCATA+TGG | + | chr1.1:5331710-5331729 | None:intergenic | 20.0% |
| !! | ATAATGACATAAAGAACTAT+TGG | - | chr1.1:5331883-5331902 | MS.gene24521:CDS | 20.0% |
| !! | ATCTATAAAATCAAGACATA+AGG | + | chr1.1:5331666-5331685 | None:intergenic | 20.0% |
| !!! | TGAATTTTCCTTATCAAATT+TGG | - | chr1.1:5332034-5332053 | MS.gene24521:CDS | 20.0% |
| !!! | TGATATGTTACTTTTTGTTT+TGG | - | chr1.1:5331712-5331731 | MS.gene24521:CDS | 20.0% |
| ! | GATAATGTTATCTTCTTCTT+CGG | + | chr1.1:5331518-5331537 | None:intergenic | 25.0% |
| ! | TAAAGAACTATTGGAATACA+AGG | - | chr1.1:5331892-5331911 | MS.gene24521:CDS | 25.0% |
| ! | TTCTTGAACCAAATTTGATA+AGG | + | chr1.1:5332045-5332064 | None:intergenic | 25.0% |
| !! | ACAAGGTTCTAGTATTAATT+GGG | - | chr1.1:5332523-5332542 | MS.gene24521:CDS | 25.0% |
| !! | TACAAGGTTCTAGTATTAAT+TGG | - | chr1.1:5332522-5332541 | MS.gene24521:CDS | 25.0% |
| AAATGGTCAATACTTTGACT+CGG | - | chr1.1:5332373-5332392 | MS.gene24521:intron | 30.0% | |
| AAGATGATGATAACTTGAAG+TGG | + | chr1.1:5332558-5332577 | None:intergenic | 30.0% | |
| AATTATCCACCAAGATTTGA+TGG | - | chr1.1:5332449-5332468 | MS.gene24521:intron | 30.0% | |
| AGAAAACTTCTTATGAAGCT+TGG | - | chr1.1:5332176-5332195 | MS.gene24521:intron | 30.0% | |
| AGTTTATGATCAAGAACATG+TGG | - | chr1.1:5332289-5332308 | MS.gene24521:intron | 30.0% | |
| ATTATCCACCAAGATTTGAT+GGG | - | chr1.1:5332450-5332469 | MS.gene24521:intron | 30.0% | |
| CAACCATCAAAAGTAGTATT+AGG | + | chr1.1:5332227-5332246 | None:intergenic | 30.0% | |
| CTTTCATACCAAACCAAAAA+GGG | + | chr1.1:5331822-5331841 | None:intergenic | 30.0% | |
| GTTTATGATCAAGAACATGT+GGG | - | chr1.1:5332290-5332309 | MS.gene24521:intron | 30.0% | |
| TGCTTCCAATACTAATGTAA+AGG | + | chr1.1:5331546-5331565 | None:intergenic | 30.0% | |
| TTTGTGTATGACAACATTGA+TGG | + | chr1.1:5332125-5332144 | None:intergenic | 30.0% | |
| ! | AAGGTTGAAAAAGAAGCTTT+TGG | - | chr1.1:5331911-5331930 | MS.gene24521:CDS | 30.0% |
| ! | AGGTTGAAAAAGAAGCTTTT+GGG | - | chr1.1:5331912-5331931 | MS.gene24521:CDS | 30.0% |
| ! | CAAGGTTCTAGTATTAATTG+GGG | - | chr1.1:5332524-5332543 | MS.gene24521:CDS | 30.0% |
| ! | TTTACATTAGTATTGGAAGC+AGG | - | chr1.1:5331545-5331564 | MS.gene24521:CDS | 30.0% |
| !! | CATCCTAATACTACTTTTGA+TGG | - | chr1.1:5332221-5332240 | MS.gene24521:intron | 30.0% |
| AAACTTCTTATGAAGCTTGG+AGG | - | chr1.1:5332179-5332198 | MS.gene24521:intron | 35.0% | |
| AATTCCAACCCATCAAATCT+TGG | + | chr1.1:5332461-5332480 | None:intergenic | 35.0% | |
| ATGTCATTATCAGTTCTTCC+TGG | + | chr1.1:5331873-5331892 | None:intergenic | 35.0% | |
| CAAGTACAATTCGCAACAAA+TGG | - | chr1.1:5332356-5332375 | MS.gene24521:intron | 35.0% | |
| CATTCCAGAGATTGATCATA+TGG | - | chr1.1:5332418-5332437 | MS.gene24521:intron | 35.0% | |
| CTTCTTGATTCCAAATACCA+AGG | - | chr1.1:5332575-5332594 | MS.gene24521:CDS | 35.0% | |
| GATACCATATGATCAATCTC+TGG | + | chr1.1:5332425-5332444 | None:intergenic | 35.0% | |
| GCTTTCATACCAAACCAAAA+AGG | + | chr1.1:5331823-5331842 | None:intergenic | 35.0% | |
| TGCAGCCTTTACATTAGTAT+TGG | - | chr1.1:5331538-5331557 | MS.gene24521:CDS | 35.0% | |
| TGCATGACATGTGGTAAATA+TGG | + | chr1.1:5332071-5332090 | None:intergenic | 35.0% | |
| TTGGTTGTGGTTGTTATCAT+TGG | + | chr1.1:5332339-5332358 | None:intergenic | 35.0% | |
| TTGTCATACACAAACCAACA+AGG | - | chr1.1:5332131-5332150 | MS.gene24521:CDS | 35.0% | |
| ! | AAACCACCATGTTTGAGATT+AGG | + | chr1.1:5331493-5331512 | None:intergenic | 35.0% |
| ! | AACCACCATGTTTGAGATTA+GGG | + | chr1.1:5331492-5331511 | None:intergenic | 35.0% |
| ! | CATTTGTTGCGAATTGTACT+TGG | + | chr1.1:5332358-5332377 | None:intergenic | 35.0% |
| ! | GGTTGAAAAAGAAGCTTTTG+GGG | - | chr1.1:5331913-5331932 | MS.gene24521:CDS | 35.0% |
| ! | TATGACAACATTGATGGTAG+TGG | + | chr1.1:5332119-5332138 | None:intergenic | 35.0% |
| ! | TGAAAAGTGAAGACCCTTTT+TGG | - | chr1.1:5331806-5331825 | MS.gene24521:CDS | 35.0% |
| !!! | GATGGGTTGGAATTCTTTTA+TGG | - | chr1.1:5332467-5332486 | MS.gene24521:intron | 35.0% |
| !!! | TTTTGGTTTGGTATGAAAGC+AGG | - | chr1.1:5331823-5331842 | MS.gene24521:CDS | 35.0% |
| ATAATTGCAGCTCAATTGCC+AGG | - | chr1.1:5331852-5331871 | MS.gene24521:CDS | 40.0% | |
| CTTGTTGCTGCTGATCTTTA+CGG | + | chr1.1:5331944-5331963 | None:intergenic | 40.0% | |
| GCTCGTAATAGAGGCAATAA+TGG | - | chr1.1:5331963-5331982 | MS.gene24521:CDS | 40.0% | |
| TTGCGAATTGTACTTGGTTG+TGG | + | chr1.1:5332352-5332371 | None:intergenic | 40.0% | |
| ! | AAGCTTGGAGGAAGATTTTC+AGG | - | chr1.1:5332191-5332210 | MS.gene24521:intron | 40.0% |
| ! | CTTGATCATTGAAGCCTTGT+TGG | + | chr1.1:5332148-5332167 | None:intergenic | 40.0% |
| !!! | AGTGAAGACCCTTTTTGGTT+TGG | - | chr1.1:5331811-5331830 | MS.gene24521:CDS | 40.0% |
| AGCAAAGGTTGCATGACATG+TGG | + | chr1.1:5332080-5332099 | None:intergenic | 45.0% | |
| CAGCAACAAGCTCGTAATAG+AGG | - | chr1.1:5331954-5331973 | MS.gene24521:CDS | 45.0% | |
| CTTCGCCCTAATCTCAAACA+TGG | - | chr1.1:5331484-5331503 | MS.gene24521:CDS | 45.0% | |
| TCCAACCCATCAAATCTTGG+TGG | + | chr1.1:5332458-5332477 | None:intergenic | 45.0% | |
| ! | TCCACCAAGATTTGATGGGT+TGG | - | chr1.1:5332454-5332473 | MS.gene24521:intron | 45.0% |
| !! | AACATTGATGGTAGTGGAGG+TGG | + | chr1.1:5332113-5332132 | None:intergenic | 45.0% |
| !! | ACATTGATGGTAGTGGAGGT+GGG | + | chr1.1:5332112-5332131 | None:intergenic | 45.0% |
| !! | GACAACATTGATGGTAGTGG+AGG | + | chr1.1:5332116-5332135 | None:intergenic | 45.0% |
| !! | TGGTTTGGTATGAAAGCAGG+TGG | - | chr1.1:5331826-5331845 | MS.gene24521:CDS | 45.0% |
| CGCCCTAATCTCAAACATGG+TGG | - | chr1.1:5331487-5331506 | MS.gene24521:CDS | 50.0% | |
| GGCGAAGATAGTTTAGCCAC+CGG | + | chr1.1:5331471-5331490 | None:intergenic | 50.0% | |
| GTGGAAAGAGTTGTCGTCTC+CGG | - | chr1.1:5331449-5331468 | MS.gene24521:CDS | 50.0% | |
| !! | ATTGATGGTAGTGGAGGTGG+GGG | + | chr1.1:5332110-5332129 | None:intergenic | 50.0% |
| !! | CATTGATGGTAGTGGAGGTG+GGG | + | chr1.1:5332111-5332130 | None:intergenic | 50.0% |
| GAAAGAGTTGTCGTCTCCGG+TGG | - | chr1.1:5331452-5331471 | MS.gene24521:CDS | 55.0% | |
| !! | GACTCGGTGCAAGAAGAGCA+AGG | - | chr1.1:5332389-5332408 | MS.gene24521:intron | 55.0% |
| !! | GGTGGGGGTGTTGATAGCAA+AGG | + | chr1.1:5332095-5332114 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 5331437 | 5332601 | 5331437 | ID=MS.gene24521 |
| chr1.1 | mRNA | 5331437 | 5332601 | 5331437 | ID=MS.gene24521.t1;Parent=MS.gene24521 |
| chr1.1 | exon | 5332472 | 5332601 | 5332472 | ID=MS.gene24521.t1.exon1;Parent=MS.gene24521.t1 |
| chr1.1 | CDS | 5332472 | 5332601 | 5332472 | ID=cds.MS.gene24521.t1;Parent=MS.gene24521.t1 |
| chr1.1 | exon | 5331437 | 5332193 | 5331437 | ID=MS.gene24521.t1.exon2;Parent=MS.gene24521.t1 |
| chr1.1 | CDS | 5331437 | 5332193 | 5331437 | ID=cds.MS.gene24521.t1;Parent=MS.gene24521.t1 |
| Gene Sequence |
| Protein sequence |
