Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52725.t1 | XP_013449672.1 | 95.4 | 218 | 10 | 0 | 1 | 218 | 1 | 218 | 8.20E-113 | 416.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52725.t1 | M1C5M7 | 82.9 | 152 | 25 | 1 | 66 | 216 | 82 | 233 | 1.0e-66 | 254.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52725.t1 | A0A072UCX0 | 95.4 | 218 | 10 | 0 | 1 | 218 | 1 | 218 | 5.9e-113 | 416.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene52725 | 0.829297 | 5.57E-55 | -1.69E-46 |
| MS.gene049171 | MS.gene52725 | 0.897065 | 2.02E-76 | -1.69E-46 |
| MS.gene049217 | MS.gene52725 | 0.804385 | 2.22E-49 | -1.69E-46 |
| MS.gene049222 | MS.gene52725 | 0.875352 | 3.30E-68 | -1.69E-46 |
| MS.gene049232 | MS.gene52725 | 0.84745 | 1.15E-59 | -1.69E-46 |
| MS.gene049257 | MS.gene52725 | 0.831422 | 1.68E-55 | -1.69E-46 |
| MS.gene049285 | MS.gene52725 | 0.859954 | 2.89E-63 | -1.69E-46 |
| MS.gene049320 | MS.gene52725 | 0.829028 | 6.47E-55 | -1.69E-46 |
| MS.gene049609 | MS.gene52725 | 0.847586 | 1.05E-59 | -1.69E-46 |
| MS.gene049627 | MS.gene52725 | 0.821135 | 4.74E-53 | -1.69E-46 |
| MS.gene049633 | MS.gene52725 | 0.839593 | 1.44E-57 | -1.69E-46 |
| MS.gene049805 | MS.gene52725 | 0.837342 | 5.50E-57 | -1.69E-46 |
| MS.gene05010 | MS.gene52725 | 0.852807 | 3.62E-61 | -1.69E-46 |
| MS.gene050163 | MS.gene52725 | 0.822458 | 2.34E-53 | -1.69E-46 |
| MS.gene050165 | MS.gene52725 | 0.80403 | 2.63E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene52725 | 0.877477 | 6.09E-69 | -1.69E-46 |
| MS.gene050323 | MS.gene52725 | 0.851257 | 9.99E-61 | -1.69E-46 |
| MS.gene050381 | MS.gene52725 | -0.804489 | 2.11E-49 | -1.69E-46 |
| MS.gene050496 | MS.gene52725 | 0.818921 | 1.52E-52 | -1.69E-46 |
| MS.gene050504 | MS.gene52725 | 0.876582 | 1.25E-68 | -1.69E-46 |
| MS.gene050518 | MS.gene52725 | 0.831854 | 1.32E-55 | -1.69E-46 |
| MS.gene050538 | MS.gene52725 | 0.834083 | 3.68E-56 | -1.69E-46 |
| MS.gene050577 | MS.gene52725 | 0.882078 | 1.41E-70 | -1.69E-46 |
| MS.gene050593 | MS.gene52725 | 0.820653 | 6.12E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene52725 | 0.828584 | 8.29E-55 | -1.69E-46 |
| MS.gene050647 | MS.gene52725 | -0.804333 | 2.27E-49 | -1.69E-46 |
| MS.gene050699 | MS.gene52725 | 0.828466 | 8.85E-55 | -1.69E-46 |
| MS.gene050747 | MS.gene52725 | 0.903259 | 4.17E-79 | -1.69E-46 |
| MS.gene050772 | MS.gene52725 | 0.844754 | 6.22E-59 | -1.69E-46 |
| MS.gene05080 | MS.gene52725 | 0.854473 | 1.20E-61 | -1.69E-46 |
| MS.gene05081 | MS.gene52725 | 0.841566 | 4.40E-58 | -1.69E-46 |
| MS.gene050868 | MS.gene52725 | 0.878336 | 3.05E-69 | -1.69E-46 |
| MS.gene050871 | MS.gene52725 | 0.879163 | 1.56E-69 | -1.69E-46 |
| MS.gene050904 | MS.gene52725 | 0.836523 | 8.90E-57 | -1.69E-46 |
| MS.gene050950 | MS.gene52725 | 0.897015 | 2.12E-76 | -1.69E-46 |
| MS.gene050986 | MS.gene52725 | 0.823953 | 1.05E-53 | -1.69E-46 |
| MS.gene05100 | MS.gene52725 | 0.822645 | 2.12E-53 | -1.69E-46 |
| MS.gene051317 | MS.gene52725 | 0.815904 | 7.28E-52 | -1.69E-46 |
| MS.gene051387 | MS.gene52725 | 0.810408 | 1.17E-50 | -1.69E-46 |
| MS.gene051551 | MS.gene52725 | 0.845406 | 4.14E-59 | -1.69E-46 |
| MS.gene051666 | MS.gene52725 | 0.828756 | 7.53E-55 | -1.69E-46 |
| MS.gene051694 | MS.gene52725 | 0.822341 | 2.49E-53 | -1.69E-46 |
| MS.gene051734 | MS.gene52725 | 0.802682 | 5.00E-49 | -1.69E-46 |
| MS.gene051838 | MS.gene52725 | 0.849017 | 4.24E-60 | -1.69E-46 |
| MS.gene051941 | MS.gene52725 | 0.806802 | 6.89E-50 | -1.69E-46 |
| MS.gene052164 | MS.gene52725 | 0.879174 | 1.54E-69 | -1.69E-46 |
| MS.gene052421 | MS.gene52725 | 0.808415 | 3.13E-50 | -1.69E-46 |
| MS.gene052482 | MS.gene52725 | 0.871711 | 5.55E-67 | -1.69E-46 |
| MS.gene052486 | MS.gene52725 | 0.867919 | 9.60E-66 | -1.69E-46 |
| MS.gene052501 | MS.gene52725 | 0.855701 | 5.29E-62 | -1.69E-46 |
| MS.gene052503 | MS.gene52725 | 0.892036 | 2.30E-74 | -1.69E-46 |
| MS.gene052505 | MS.gene52725 | 0.83875 | 2.39E-57 | -1.69E-46 |
| MS.gene052506 | MS.gene52725 | 0.835186 | 1.94E-56 | -1.69E-46 |
| MS.gene052615 | MS.gene52725 | 0.83199 | 1.22E-55 | -1.69E-46 |
| MS.gene052822 | MS.gene52725 | 0.827194 | 1.79E-54 | -1.69E-46 |
| MS.gene052823 | MS.gene52725 | 0.892237 | 1.91E-74 | -1.69E-46 |
| MS.gene052825 | MS.gene52725 | 0.889821 | 1.72E-73 | -1.69E-46 |
| MS.gene052966 | MS.gene52725 | 0.847595 | 1.05E-59 | -1.69E-46 |
| MS.gene052982 | MS.gene52725 | 0.832098 | 1.15E-55 | -1.69E-46 |
| MS.gene053203 | MS.gene52725 | 0.882313 | 1.16E-70 | -1.69E-46 |
| MS.gene053207 | MS.gene52725 | 0.818389 | 2.01E-52 | -1.69E-46 |
| MS.gene053213 | MS.gene52725 | 0.855568 | 5.78E-62 | -1.69E-46 |
| MS.gene053286 | MS.gene52725 | 0.856765 | 2.58E-62 | -1.69E-46 |
| MS.gene053436 | MS.gene52725 | 0.871256 | 7.85E-67 | -1.69E-46 |
| MS.gene053479 | MS.gene52725 | 0.854873 | 9.21E-62 | -1.69E-46 |
| MS.gene053480 | MS.gene52725 | 0.84081 | 6.95E-58 | -1.69E-46 |
| MS.gene053574 | MS.gene52725 | 0.869853 | 2.27E-66 | -1.69E-46 |
| MS.gene053579 | MS.gene52725 | 0.807855 | 4.12E-50 | -1.69E-46 |
| MS.gene053626 | MS.gene52725 | 0.872099 | 4.13E-67 | -1.69E-46 |
| MS.gene053632 | MS.gene52725 | 0.816166 | 6.36E-52 | -1.69E-46 |
| MS.gene053824 | MS.gene52725 | 0.870246 | 1.69E-66 | -1.69E-46 |
| MS.gene053997 | MS.gene52725 | 0.802733 | 4.88E-49 | -1.69E-46 |
| MS.gene054003 | MS.gene52725 | 0.815161 | 1.07E-51 | -1.69E-46 |
| MS.gene054408 | MS.gene52725 | 0.903453 | 3.41E-79 | -1.69E-46 |
| MS.gene054595 | MS.gene52725 | 0.880121 | 7.13E-70 | -1.69E-46 |
| MS.gene054600 | MS.gene52725 | 0.833936 | 4.01E-56 | -1.69E-46 |
| MS.gene054642 | MS.gene52725 | 0.818039 | 2.41E-52 | -1.69E-46 |
| MS.gene054706 | MS.gene52725 | -0.821574 | 3.76E-53 | -1.69E-46 |
| MS.gene05470 | MS.gene52725 | 0.894157 | 3.21E-75 | -1.69E-46 |
| MS.gene054927 | MS.gene52725 | 0.849129 | 3.94E-60 | -1.69E-46 |
| MS.gene054954 | MS.gene52725 | 0.851025 | 1.16E-60 | -1.69E-46 |
| MS.gene05505 | MS.gene52725 | 0.802234 | 6.18E-49 | -1.69E-46 |
| MS.gene055206 | MS.gene52725 | 0.802871 | 4.57E-49 | -1.69E-46 |
| MS.gene055224 | MS.gene52725 | 0.831762 | 1.39E-55 | -1.69E-46 |
| MS.gene055289 | MS.gene52725 | 0.813534 | 2.44E-51 | -1.69E-46 |
| MS.gene055362 | MS.gene52725 | 0.851694 | 7.51E-61 | -1.69E-46 |
| MS.gene055502 | MS.gene52725 | 0.875676 | 2.55E-68 | -1.69E-46 |
| MS.gene055577 | MS.gene52725 | 0.857781 | 1.29E-62 | -1.69E-46 |
| MS.gene055629 | MS.gene52725 | 0.832752 | 7.90E-56 | -1.69E-46 |
| MS.gene055722 | MS.gene52725 | 0.806773 | 6.99E-50 | -1.69E-46 |
| MS.gene055748 | MS.gene52725 | 0.856518 | 3.05E-62 | -1.69E-46 |
| MS.gene055750 | MS.gene52725 | 0.847286 | 1.27E-59 | -1.69E-46 |
| MS.gene055751 | MS.gene52725 | 0.843786 | 1.13E-58 | -1.69E-46 |
| MS.gene05589 | MS.gene52725 | 0.884659 | 1.59E-71 | -1.69E-46 |
| MS.gene055941 | MS.gene52725 | 0.821991 | 3.01E-53 | -1.69E-46 |
| MS.gene056040 | MS.gene52725 | 0.890841 | 6.84E-74 | -1.69E-46 |
| MS.gene056166 | MS.gene52725 | 0.870754 | 1.15E-66 | -1.69E-46 |
| MS.gene056218 | MS.gene52725 | -0.80292 | 4.46E-49 | -1.69E-46 |
| MS.gene056374 | MS.gene52725 | 0.819058 | 1.42E-52 | -1.69E-46 |
| MS.gene056409 | MS.gene52725 | 0.80956 | 1.78E-50 | -1.69E-46 |
| MS.gene056446 | MS.gene52725 | 0.848248 | 6.92E-60 | -1.69E-46 |
| MS.gene056504 | MS.gene52725 | 0.875842 | 2.24E-68 | -1.69E-46 |
| MS.gene056510 | MS.gene52725 | 0.857369 | 1.71E-62 | -1.69E-46 |
| MS.gene056610 | MS.gene52725 | 0.836906 | 7.11E-57 | -1.69E-46 |
| MS.gene056647 | MS.gene52725 | -0.811844 | 5.71E-51 | -1.69E-46 |
| MS.gene056657 | MS.gene52725 | 0.826819 | 2.20E-54 | -1.69E-46 |
| MS.gene056692 | MS.gene52725 | 0.836856 | 7.32E-57 | -1.69E-46 |
| MS.gene056878 | MS.gene52725 | 0.814998 | 1.16E-51 | -1.69E-46 |
| MS.gene057019 | MS.gene52725 | 0.803457 | 3.45E-49 | -1.69E-46 |
| MS.gene057048 | MS.gene52725 | 0.818347 | 2.06E-52 | -1.69E-46 |
| MS.gene057086 | MS.gene52725 | 0.819329 | 1.23E-52 | -1.69E-46 |
| MS.gene057416 | MS.gene52725 | 0.842819 | 2.05E-58 | -1.69E-46 |
| MS.gene057658 | MS.gene52725 | 0.873064 | 1.96E-67 | -1.69E-46 |
| MS.gene057831 | MS.gene52725 | 0.84724 | 1.31E-59 | -1.69E-46 |
| MS.gene057856 | MS.gene52725 | 0.814947 | 1.19E-51 | -1.69E-46 |
| MS.gene05787 | MS.gene52725 | 0.869457 | 3.05E-66 | -1.69E-46 |
| MS.gene058019 | MS.gene52725 | 0.800353 | 1.50E-48 | -1.69E-46 |
| MS.gene058130 | MS.gene52725 | 0.82353 | 1.32E-53 | -1.69E-46 |
| MS.gene058157 | MS.gene52725 | 0.82417 | 9.33E-54 | -1.69E-46 |
| MS.gene058334 | MS.gene52725 | -0.806876 | 6.65E-50 | -1.69E-46 |
| MS.gene058338 | MS.gene52725 | 0.821393 | 4.13E-53 | -1.69E-46 |
| MS.gene05837 | MS.gene52725 | 0.884794 | 1.41E-71 | -1.69E-46 |
| MS.gene058419 | MS.gene52725 | 0.855879 | 4.69E-62 | -1.69E-46 |
| MS.gene058444 | MS.gene52725 | 0.812082 | 5.07E-51 | -1.69E-46 |
| MS.gene058544 | MS.gene52725 | 0.888955 | 3.73E-73 | -1.69E-46 |
| MS.gene058700 | MS.gene52725 | 0.801885 | 7.29E-49 | -1.69E-46 |
| MS.gene058773 | MS.gene52725 | 0.839074 | 1.97E-57 | -1.69E-46 |
| MS.gene058838 | MS.gene52725 | 0.874433 | 6.78E-68 | -1.69E-46 |
| MS.gene059022 | MS.gene52725 | 0.851068 | 1.13E-60 | -1.69E-46 |
| MS.gene059023 | MS.gene52725 | 0.887942 | 9.16E-73 | -1.69E-46 |
| MS.gene059103 | MS.gene52725 | -0.808762 | 2.64E-50 | -1.69E-46 |
| MS.gene05914 | MS.gene52725 | 0.845139 | 4.89E-59 | -1.69E-46 |
| MS.gene059303 | MS.gene52725 | 0.810685 | 1.02E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene52725 | 0.815886 | 7.35E-52 | -1.69E-46 |
| MS.gene059367 | MS.gene52725 | 0.802735 | 4.87E-49 | -1.69E-46 |
| MS.gene059480 | MS.gene52725 | -0.809291 | 2.04E-50 | -1.69E-46 |
| MS.gene059667 | MS.gene52725 | 0.890956 | 6.16E-74 | -1.69E-46 |
| MS.gene059677 | MS.gene52725 | 0.842655 | 2.26E-58 | -1.69E-46 |
| MS.gene059718 | MS.gene52725 | 0.863542 | 2.31E-64 | -1.69E-46 |
| MS.gene059756 | MS.gene52725 | 0.861453 | 1.02E-63 | -1.69E-46 |
| MS.gene059801 | MS.gene52725 | 0.846592 | 1.97E-59 | -1.69E-46 |
| MS.gene059837 | MS.gene52725 | 0.813823 | 2.11E-51 | -1.69E-46 |
| MS.gene059865 | MS.gene52725 | 0.842621 | 2.31E-58 | -1.69E-46 |
| MS.gene05996 | MS.gene52725 | 0.801489 | 8.79E-49 | -1.69E-46 |
| MS.gene06000 | MS.gene52725 | 0.869637 | 2.67E-66 | -1.69E-46 |
| MS.gene06027 | MS.gene52725 | 0.812623 | 3.86E-51 | -1.69E-46 |
| MS.gene060482 | MS.gene52725 | 0.805841 | 1.10E-49 | -1.69E-46 |
| MS.gene060595 | MS.gene52725 | -0.81698 | 4.18E-52 | -1.69E-46 |
| MS.gene060762 | MS.gene52725 | 0.860573 | 1.88E-63 | -1.69E-46 |
| MS.gene060778 | MS.gene52725 | 0.845346 | 4.30E-59 | -1.69E-46 |
| MS.gene06077 | MS.gene52725 | 0.896682 | 2.92E-76 | -1.69E-46 |
| MS.gene06092 | MS.gene52725 | 0.882767 | 7.90E-71 | -1.69E-46 |
| MS.gene06097 | MS.gene52725 | 0.835938 | 1.25E-56 | -1.69E-46 |
| MS.gene060982 | MS.gene52725 | 0.859188 | 4.92E-63 | -1.69E-46 |
| MS.gene061150 | MS.gene52725 | 0.859459 | 4.08E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52725.t1 | MTR_7g094220 | 95.413 | 218 | 10 | 0 | 1 | 218 | 1 | 218 | 2.11e-146 | 406 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52725.t1 | AT2G48070 | 80.282 | 142 | 28 | 0 | 77 | 218 | 56 | 197 | 4.11e-84 | 248 |
| MS.gene52725.t1 | AT2G48070 | 80.282 | 142 | 28 | 0 | 77 | 218 | 56 | 197 | 4.11e-84 | 248 |
| MS.gene52725.t1 | AT2G48070 | 78.082 | 146 | 28 | 1 | 77 | 218 | 56 | 201 | 9.15e-82 | 242 |
Find 52 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCAAATCATCACCTTTAT+AGG | 0.127789 | 7.2:-15248172 | MS.gene52725:CDS |
| CTTCAGACCAACCTGATCTT+TGG | 0.218609 | 7.2:-15247348 | MS.gene52725:CDS |
| GCAGATATAATAGCTTTCTT+TGG | 0.277583 | 7.2:-15246587 | MS.gene52725:CDS |
| CTCAAATCATCACCTTTATA+GGG | 0.327309 | 7.2:-15248171 | MS.gene52725:CDS |
| AATAGACAAAGAACTTAAAA+AGG | 0.327859 | 7.2:-15247964 | MS.gene52725:intron |
| TGATGGGAATGTGGTCTGTT+TGG | 0.374261 | 7.2:-15247321 | MS.gene52725:CDS |
| ACCTGATCTTTGGAGATTGA+TGG | 0.394975 | 7.2:-15247338 | MS.gene52725:CDS |
| CTTAAGGTTGAAAGGTACTT+TGG | 0.417588 | 7.2:+15248101 | None:intergenic |
| TGAAGTCCTTTGCTGTTGTT+TGG | 0.424798 | 7.2:-15246613 | MS.gene52725:CDS |
| TGAAAGGTACTTTGGAAGGT+TGG | 0.424998 | 7.2:+15248109 | None:intergenic |
| AAGGGTAGGGTACCCTATAA+AGG | 0.426575 | 7.2:+15248159 | None:intergenic |
| GGGATAAGGTGAGCGTCTTA+AGG | 0.439792 | 7.2:+15248085 | None:intergenic |
| AGCGTATGTATCAATGTTGT+TGG | 0.442954 | 7.2:-15247401 | MS.gene52725:CDS |
| TGCTGCAACCTTTGCACCGA+GGG | 0.448437 | 7.2:-15247488 | MS.gene52725:CDS |
| ATTTGAAGTGGCACATGCAA+TGG | 0.456020 | 7.2:+15248064 | None:intergenic |
| CAACATTGAGCAAGGGAACT+AGG | 0.458291 | 7.2:+15246651 | None:intergenic |
| CCTATAAAGGTGATGATTTG+AGG | 0.463442 | 7.2:+15248172 | None:intergenic |
| ACAGTATACAAAGTACTTCC+AGG | 0.467276 | 7.2:+15247436 | None:intergenic |
| CTTTGGAATGTATGCATGGA+AGG | 0.470844 | 7.2:-15246570 | MS.gene52725:intron |
| TTTGAACTGGAGAGACTAAA+GGG | 0.474327 | 7.2:+15248141 | None:intergenic |
| GCTTGGATGGCTCCAGAAAA+CGG | 0.476983 | 7.2:-15246394 | MS.gene52725:CDS |
| CCCATCAATCTCCAAAGATC+AGG | 0.478700 | 7.2:+15247337 | None:intergenic |
| TTTGATGCCGTCGAAGCCCT+CGG | 0.481915 | 7.2:+15247472 | None:intergenic |
| TACAACTTCTTCATTTGAAG+TGG | 0.486651 | 7.2:+15248052 | None:intergenic |
| CTTTGCACCGAGGGCTTCGA+CGG | 0.486958 | 7.2:-15247479 | MS.gene52725:CDS |
| TTTGAAGTGGCACATGCAAT+GGG | 0.489031 | 7.2:+15248065 | None:intergenic |
| AAAGGTGATGATTTGAGGAA+TGG | 0.491588 | 7.2:+15248177 | None:intergenic |
| AAAGTACTTCCAGGTACTGC+AGG | 0.496122 | 7.2:+15247445 | None:intergenic |
| GTTTGAACTGGAGAGACTAA+AGG | 0.497130 | 7.2:+15248140 | None:intergenic |
| CCTGATCTTTGGAGATTGAT+GGG | 0.498697 | 7.2:-15247337 | MS.gene52725:CDS |
| CAAAGATCAGGTTGGTCTGA+AGG | 0.501488 | 7.2:+15247349 | None:intergenic |
| GTCCTTTGCTGTTGTTTGGT+CGG | 0.519274 | 7.2:-15246609 | MS.gene52725:CDS |
| GTGGCACATGCAATGGGATA+AGG | 0.520683 | 7.2:+15248071 | None:intergenic |
| CAATCTCGAGCTCGCAAGGA+AGG | 0.524706 | 7.2:+15246713 | None:intergenic |
| AGGTTGAAAGGTACTTTGGA+AGG | 0.535613 | 7.2:+15248105 | None:intergenic |
| AAAGATCAGGTTGGTCTGAA+GGG | 0.540412 | 7.2:+15247350 | None:intergenic |
| TGAGCAATCTCGAGCTCGCA+AGG | 0.542578 | 7.2:+15246709 | None:intergenic |
| CGTCGAAGCCCTCGGTGCAA+AGG | 0.546826 | 7.2:+15247480 | None:intergenic |
| CTTTCTTTGGAATGTATGCA+TGG | 0.570202 | 7.2:-15246574 | MS.gene52725:CDS |
| GCGTATGTATCAATGTTGTT+GGG | 0.573235 | 7.2:-15247400 | MS.gene52725:CDS |
| TGGGATTGCAACATTGAGCA+AGG | 0.584925 | 7.2:+15246643 | None:intergenic |
| GTGAGCGTCTTAAGGTTGAA+AGG | 0.597321 | 7.2:+15248093 | None:intergenic |
| AACTGGAGAGACTAAAGGGT+AGG | 0.601028 | 7.2:+15248145 | None:intergenic |
| AAGTACTTCCAGGTACTGCA+GGG | 0.609643 | 7.2:+15247446 | None:intergenic |
| TTTGGAGATTGATGGGAATG+TGG | 0.613525 | 7.2:-15247330 | MS.gene52725:CDS |
| TGCCGACCAAACAACAGCAA+AGG | 0.622540 | 7.2:+15246607 | None:intergenic |
| ACTGGAGAGACTAAAGGGTA+GGG | 0.645359 | 7.2:+15248146 | None:intergenic |
| TCAATCTCCAAAGATCAGGT+TGG | 0.648619 | 7.2:+15247341 | None:intergenic |
| TATGTATCAATGTTGTTGGG+TGG | 0.657784 | 7.2:-15247397 | MS.gene52725:CDS |
| GGGATTGCAACATTGAGCAA+GGG | 0.659930 | 7.2:+15246644 | None:intergenic |
| CTGCTGCAACCTTTGCACCG+AGG | 0.681085 | 7.2:-15247489 | MS.gene52725:CDS |
| CATTGAGCAAGGGAACTAGG+AGG | 0.725133 | 7.2:+15246654 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATATGAGAAAGAATATT+AGG | - | chr7.2:15247432-15247451 | MS.gene52725:CDS | 15.0% |
| !! | AATAGACAAAGAACTTAAAA+AGG | - | chr7.2:15246616-15246635 | MS.gene52725:CDS | 20.0% |
| !! | ATTAGAAAGATAGAGAAAAA+TGG | - | chr7.2:15247019-15247038 | MS.gene52725:intron | 20.0% |
| !! | TCAAAATCAGGATAAAAAAT+TGG | + | chr7.2:15247308-15247327 | None:intergenic | 20.0% |
| !! | TTCTTTCTCATATTTACAAA+AGG | + | chr7.2:15247428-15247447 | None:intergenic | 20.0% |
| !!! | AAGCATAGATTTTTACAAAA+TGG | + | chr7.2:15246708-15246727 | None:intergenic | 20.0% |
| !!! | AGCATAGATTTTTACAAAAT+GGG | + | chr7.2:15246707-15246726 | None:intergenic | 20.0% |
| !!! | TCAATTCAAATCGTATTTTA+AGG | - | chr7.2:15246678-15246697 | MS.gene52725:CDS | 20.0% |
| !!! | TTTTTAAGTTCTTTGTCTAT+TGG | + | chr7.2:15246617-15246636 | None:intergenic | 20.0% |
| ! | AATCAGGATAAAAAATTGGT+AGG | + | chr7.2:15247304-15247323 | None:intergenic | 25.0% |
| ! | AATTACAAGCTAAATTCGAT+GGG | + | chr7.2:15247484-15247503 | None:intergenic | 25.0% |
| ! | ATTGAAGGATTATAGAAAGA+TGG | + | chr7.2:15246656-15246675 | None:intergenic | 25.0% |
| ! | CGATTTGAATTGAAAATTGA+AGG | + | chr7.2:15246671-15246690 | None:intergenic | 25.0% |
| ! | CTTAGTGTTATAAGTTCTTT+AGG | - | chr7.2:15246819-15246838 | MS.gene52725:intron | 25.0% |
| ! | CTTTACTTGGAGTAAAAAAT+TGG | - | chr7.2:15247808-15247827 | MS.gene52725:intron | 25.0% |
| ! | GATAGCTTATGAACATAATT+TGG | - | chr7.2:15246841-15246860 | MS.gene52725:intron | 25.0% |
| ! | TTTATGCAACTTTCAAAATC+AGG | + | chr7.2:15247320-15247339 | None:intergenic | 25.0% |
| !! | ATCGTCAAATATTGATAACA+TGG | + | chr7.2:15248149-15248168 | None:intergenic | 25.0% |
| !! | GAAATGTTTGTTGAATTGAT+AGG | - | chr7.2:15247056-15247075 | MS.gene52725:intron | 25.0% |
| !!! | ATATGTTAATTTGTCACCAT+TGG | + | chr7.2:15247576-15247595 | None:intergenic | 25.0% |
| !!! | GCTTTTCTAAAAATTGATCA+AGG | - | chr7.2:15246724-15246743 | MS.gene52725:intron | 25.0% |
| !!! | TATGTTAATTTGTCACCATT+GGG | + | chr7.2:15247575-15247594 | None:intergenic | 25.0% |
| CAAAGGACTTCAAAAAGAAT+GGG | + | chr7.2:15247959-15247978 | None:intergenic | 30.0% | |
| CAATTACAAGCTAAATTCGA+TGG | + | chr7.2:15247485-15247504 | None:intergenic | 30.0% | |
| CATTGCTATGATTCTTTACT+TGG | - | chr7.2:15247795-15247814 | MS.gene52725:intron | 30.0% | |
| CTCAAATCATCACCTTTATA+GGG | - | chr7.2:15246409-15246428 | MS.gene52725:intron | 30.0% | |
| GACAAAGAACTTAAAAAGGT+TGG | - | chr7.2:15246620-15246639 | MS.gene52725:CDS | 30.0% | |
| GCAGATATAATAGCTTTCTT+TGG | - | chr7.2:15247993-15248012 | MS.gene52725:CDS | 30.0% | |
| GGTGACAAATTAACATATCT+AGG | - | chr7.2:15247578-15247597 | MS.gene52725:intron | 30.0% | |
| GTAATACTGAAGTCTAACAT+TGG | + | chr7.2:15247553-15247572 | None:intergenic | 30.0% | |
| TAAGCTAGATAAAAGACGTA+AGG | + | chr7.2:15247676-15247695 | None:intergenic | 30.0% | |
| TACAACTTCTTCATTTGAAG+TGG | + | chr7.2:15246531-15246550 | None:intergenic | 30.0% | |
| ! | ATGTTTGTTGAATTGATAGG+TGG | - | chr7.2:15247059-15247078 | MS.gene52725:intron | 30.0% |
| ! | GAAGGTACATACGATTTATT+AGG | - | chr7.2:15248028-15248047 | MS.gene52725:CDS | 30.0% |
| ! | TTTAGTTTATTGCTCGTTAG+AGG | + | chr7.2:15248061-15248080 | None:intergenic | 30.0% |
| !! | TCTTTTATCTAGCTTATGTG+TGG | - | chr7.2:15247680-15247699 | MS.gene52725:intron | 30.0% |
| AAAGGTGATGATTTGAGGAA+TGG | + | chr7.2:15246406-15246425 | None:intergenic | 35.0% | |
| AACAAGAGCTTCAGTTAGAA+AGG | - | chr7.2:15246958-15246977 | MS.gene52725:intron | 35.0% | |
| ACATTCAGCAGAATGATAAC+TGG | + | chr7.2:15247376-15247395 | None:intergenic | 35.0% | |
| AGCGTATGTATCAATGTTGT+TGG | - | chr7.2:15247179-15247198 | MS.gene52725:intron | 35.0% | |
| CCTATAAAGGTGATGATTTG+AGG | + | chr7.2:15246411-15246430 | None:intergenic | 35.0% | |
| CCTCAAATCATCACCTTTAT+AGG | - | chr7.2:15246408-15246427 | MS.gene52725:intron | 35.0% | |
| CTGCTGAATGTTTGATTGAT+CGG | - | chr7.2:15247385-15247404 | MS.gene52725:CDS | 35.0% | |
| GCAAAGGACTTCAAAAAGAA+TGG | + | chr7.2:15247960-15247979 | None:intergenic | 35.0% | |
| GCGTATGTATCAATGTTGTT+GGG | - | chr7.2:15247180-15247199 | MS.gene52725:intron | 35.0% | |
| TAGACTTCAGTATTACCCAA+TGG | - | chr7.2:15247557-15247576 | MS.gene52725:intron | 35.0% | |
| TATGTATCAATGTTGTTGGG+TGG | - | chr7.2:15247183-15247202 | MS.gene52725:intron | 35.0% | |
| TATTGGAGTAGAAAAAGCAG+AGG | + | chr7.2:15246600-15246619 | None:intergenic | 35.0% | |
| TTGCATAAATGAACAAGTGC+TGG | - | chr7.2:15247331-15247350 | MS.gene52725:CDS | 35.0% | |
| TTTGAACTGGAGAGACTAAA+GGG | + | chr7.2:15246442-15246461 | None:intergenic | 35.0% | |
| ! | ACAGTATACAAAGTACTTCC+AGG | + | chr7.2:15247147-15247166 | None:intergenic | 35.0% |
| ! | AGTGTTTTCTTCATTGGTGT+TGG | + | chr7.2:15246576-15246595 | None:intergenic | 35.0% |
| ! | ATGTTGCTTGAGAGTAAATG+AGG | - | chr7.2:15246994-15247013 | MS.gene52725:intron | 35.0% |
| ! | TGTTGCTTGAGAGTAAATGA+GGG | - | chr7.2:15246995-15247014 | MS.gene52725:intron | 35.0% |
| !! | CTTAAGGTTGAAAGGTACTT+TGG | + | chr7.2:15246482-15246501 | None:intergenic | 35.0% |
| !! | CTTTCTTTGGAATGTATGCA+TGG | - | chr7.2:15248006-15248025 | MS.gene52725:CDS | 35.0% |
| !! | TAAATGAACAAGTGCTGGAA+TGG | - | chr7.2:15247336-15247355 | MS.gene52725:CDS | 35.0% |
| !!! | AGAGGAAGTGTTTTCTTCAT+TGG | + | chr7.2:15246582-15246601 | None:intergenic | 35.0% |
| !!! | TTGGTTTTGTTGGTTTGAAC+TGG | + | chr7.2:15246455-15246474 | None:intergenic | 35.0% |
| AGCAATGAGATCTTGCAAGA+AGG | + | chr7.2:15247782-15247801 | None:intergenic | 40.0% | |
| GTTTGAACTGGAGAGACTAA+AGG | + | chr7.2:15246443-15246462 | None:intergenic | 40.0% | |
| TAAACCAGACTTGTAAGACG+AGG | - | chr7.2:15247646-15247665 | MS.gene52725:intron | 40.0% | |
| TCAATCTCCAAAGATCAGGT+TGG | + | chr7.2:15247242-15247261 | None:intergenic | 40.0% | |
| TTTGGAGATTGATGGGAATG+TGG | - | chr7.2:15247250-15247269 | MS.gene52725:intron | 40.0% | |
| ! | ACCTGATCTTTGGAGATTGA+TGG | - | chr7.2:15247242-15247261 | MS.gene52725:intron | 40.0% |
| ! | ATCTTCCTTTTCTTGCAGCT+TGG | - | chr7.2:15248169-15248188 | MS.gene52725:CDS | 40.0% |
| ! | ATTTGAAGTGGCACATGCAA+TGG | + | chr7.2:15246519-15246538 | None:intergenic | 40.0% |
| ! | CCTGATCTTTGGAGATTGAT+GGG | - | chr7.2:15247243-15247262 | MS.gene52725:intron | 40.0% |
| ! | CGTCTTACAAGTCTGGTTTA+TGG | + | chr7.2:15247646-15247665 | None:intergenic | 40.0% |
| ! | TTTGAAGTGGCACATGCAAT+GGG | + | chr7.2:15246518-15246537 | None:intergenic | 40.0% |
| !! | AAAGATCAGGTTGGTCTGAA+GGG | + | chr7.2:15247233-15247252 | None:intergenic | 40.0% |
| !! | AGGTTGAAAGGTACTTTGGA+AGG | + | chr7.2:15246478-15246497 | None:intergenic | 40.0% |
| !! | CTTTGGAATGTATGCATGGA+AGG | - | chr7.2:15248010-15248029 | MS.gene52725:CDS | 40.0% |
| !! | TGAAAGGTACTTTGGAAGGT+TGG | + | chr7.2:15246474-15246493 | None:intergenic | 40.0% |
| !! | TGAAGTCCTTTGCTGTTGTT+TGG | - | chr7.2:15247967-15247986 | MS.gene52725:CDS | 40.0% |
| !!! | CTTTGGAAGGTTGGTTTTGT+TGG | + | chr7.2:15246465-15246484 | None:intergenic | 40.0% |
| AACTGGAGAGACTAAAGGGT+AGG | + | chr7.2:15246438-15246457 | None:intergenic | 45.0% | |
| ACTGGAGAGACTAAAGGGTA+GGG | + | chr7.2:15246437-15246456 | None:intergenic | 45.0% | |
| CAACATTGAGCAAGGGAACT+AGG | + | chr7.2:15247932-15247951 | None:intergenic | 45.0% | |
| CAATCCTCGTCTTACAAGTC+TGG | + | chr7.2:15247653-15247672 | None:intergenic | 45.0% | |
| CCCATCAATCTCCAAAGATC+AGG | + | chr7.2:15247246-15247265 | None:intergenic | 45.0% | |
| CTTCAGACCAACCTGATCTT+TGG | - | chr7.2:15247232-15247251 | MS.gene52725:intron | 45.0% | |
| GAAGGAGCTGGAACAACTAT+TGG | + | chr7.2:15247764-15247783 | None:intergenic | 45.0% | |
| GGGATTGCAACATTGAGCAA+GGG | + | chr7.2:15247939-15247958 | None:intergenic | 45.0% | |
| GTGAGCGTCTTAAGGTTGAA+AGG | + | chr7.2:15246490-15246509 | None:intergenic | 45.0% | |
| TCAAAAAACCCTGCAGTACC+TGG | - | chr7.2:15247126-15247145 | MS.gene52725:intron | 45.0% | |
| TGATGGGAATGTGGTCTGTT+TGG | - | chr7.2:15247259-15247278 | MS.gene52725:intron | 45.0% | |
| TGGGATTGCAACATTGAGCA+AGG | + | chr7.2:15247940-15247959 | None:intergenic | 45.0% | |
| ! | AAAGTACTTCCAGGTACTGC+AGG | + | chr7.2:15247138-15247157 | None:intergenic | 45.0% |
| ! | AAGGGTAGGGTACCCTATAA+AGG | + | chr7.2:15246424-15246443 | None:intergenic | 45.0% |
| ! | AAGTACTTCCAGGTACTGCA+GGG | + | chr7.2:15247137-15247156 | None:intergenic | 45.0% |
| ! | TCCTTTTCTTGCAGCTTGGA+TGG | - | chr7.2:15248173-15248192 | MS.gene52725:CDS | 45.0% |
| !! | CAAAGATCAGGTTGGTCTGA+AGG | + | chr7.2:15247234-15247253 | None:intergenic | 45.0% |
| !! | GTCCTTTGCTGTTGTTTGGT+CGG | - | chr7.2:15247971-15247990 | MS.gene52725:CDS | 45.0% |
| CATTGAGCAAGGGAACTAGG+AGG | + | chr7.2:15247929-15247948 | None:intergenic | 50.0% | |
| GAGATCTTGCAAGAAGGAGC+TGG | + | chr7.2:15247776-15247795 | None:intergenic | 50.0% | |
| GCCATCCAAGCTGCAAGAAA+AGG | + | chr7.2:15248177-15248196 | None:intergenic | 50.0% | |
| GCTTGGATGGCTCCAGAAAA+CGG | - | chr7.2:15248186-15248205 | MS.gene52725:CDS | 50.0% | |
| GGGATAAGGTGAGCGTCTTA+AGG | + | chr7.2:15246498-15246517 | None:intergenic | 50.0% | |
| GTGGCACATGCAATGGGATA+AGG | + | chr7.2:15246512-15246531 | None:intergenic | 50.0% | |
| TGCCGACCAAACAACAGCAA+AGG | + | chr7.2:15247976-15247995 | None:intergenic | 50.0% | |
| CAATCTCGAGCTCGCAAGGA+AGG | + | chr7.2:15247870-15247889 | None:intergenic | 55.0% | |
| TGAGCAATCTCGAGCTCGCA+AGG | + | chr7.2:15247874-15247893 | None:intergenic | 55.0% | |
| TGCTGCAACCTTTGCACCGA+GGG | - | chr7.2:15247092-15247111 | MS.gene52725:intron | 55.0% | |
| TTTGATGCCGTCGAAGCCCT+CGG | + | chr7.2:15247111-15247130 | None:intergenic | 55.0% | |
| CTGCTGCAACCTTTGCACCG+AGG | - | chr7.2:15247091-15247110 | MS.gene52725:intron | 60.0% | |
| CTTTGCACCGAGGGCTTCGA+CGG | - | chr7.2:15247101-15247120 | MS.gene52725:intron | 60.0% | |
| ! | CGTCGAAGCCCTCGGTGCAA+AGG | + | chr7.2:15247103-15247122 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 15246389 | 15248213 | 15246389 | ID=MS.gene52725 |
| chr7.2 | mRNA | 15246389 | 15248213 | 15246389 | ID=MS.gene52725.t1;Parent=MS.gene52725 |
| chr7.2 | exon | 15247965 | 15248213 | 15247965 | ID=MS.gene52725.t1.exon1;Parent=MS.gene52725.t1 |
| chr7.2 | CDS | 15247965 | 15248213 | 15247965 | ID=cds.MS.gene52725.t1;Parent=MS.gene52725.t1 |
| chr7.2 | exon | 15247314 | 15247524 | 15247314 | ID=MS.gene52725.t1.exon2;Parent=MS.gene52725.t1 |
| chr7.2 | CDS | 15247314 | 15247524 | 15247314 | ID=cds.MS.gene52725.t1;Parent=MS.gene52725.t1 |
| chr7.2 | exon | 15246571 | 15246740 | 15246571 | ID=MS.gene52725.t1.exon3;Parent=MS.gene52725.t1 |
| chr7.2 | CDS | 15246571 | 15246740 | 15246571 | ID=cds.MS.gene52725.t1;Parent=MS.gene52725.t1 |
| chr7.2 | exon | 15246389 | 15246415 | 15246389 | ID=MS.gene52725.t1.exon4;Parent=MS.gene52725.t1 |
| chr7.2 | CDS | 15246389 | 15246415 | 15246389 | ID=cds.MS.gene52725.t1;Parent=MS.gene52725.t1 |
| Gene Sequence |
| Protein sequence |
