Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79114.t1 | XP_003601843.1 | 81.9 | 182 | 4 | 2 | 1 | 155 | 1 | 180 | 2.10E-49 | 205.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79114.t1 | Q8LDL0 | 66.4 | 110 | 10 | 1 | 73 | 155 | 73 | 182 | 2.3e-33 | 143.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79114.t1 | G7J2I7 | 81.9 | 182 | 4 | 2 | 1 | 155 | 1 | 180 | 1.5e-49 | 205.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene79114 | 0.865236 | 6.84E-65 | -1.69E-46 |
| MS.gene049171 | MS.gene79114 | 0.845994 | 2.87E-59 | -1.69E-46 |
| MS.gene049217 | MS.gene79114 | 0.835566 | 1.56E-56 | -1.69E-46 |
| MS.gene049222 | MS.gene79114 | 0.88745 | 1.41E-72 | -1.69E-46 |
| MS.gene049232 | MS.gene79114 | 0.82834 | 9.49E-55 | -1.69E-46 |
| MS.gene049285 | MS.gene79114 | 0.847283 | 1.28E-59 | -1.69E-46 |
| MS.gene049320 | MS.gene79114 | 0.841159 | 5.63E-58 | -1.69E-46 |
| MS.gene049427 | MS.gene79114 | 0.828676 | 7.88E-55 | -1.69E-46 |
| MS.gene049609 | MS.gene79114 | 0.838457 | 2.84E-57 | -1.69E-46 |
| MS.gene049615 | MS.gene79114 | 0.810804 | 9.61E-51 | -1.69E-46 |
| MS.gene049633 | MS.gene79114 | 0.803361 | 3.62E-49 | -1.69E-46 |
| MS.gene049805 | MS.gene79114 | 0.86358 | 2.25E-64 | -1.69E-46 |
| MS.gene05010 | MS.gene79114 | 0.815854 | 7.47E-52 | -1.69E-46 |
| MS.gene050163 | MS.gene79114 | 0.838204 | 3.30E-57 | -1.69E-46 |
| MS.gene050165 | MS.gene79114 | 0.811939 | 5.45E-51 | -1.69E-46 |
| MS.gene050323 | MS.gene79114 | 0.809329 | 2.00E-50 | -1.69E-46 |
| MS.gene050333 | MS.gene79114 | 0.800996 | 1.11E-48 | -1.69E-46 |
| MS.gene050380 | MS.gene79114 | -0.814765 | 1.30E-51 | -1.69E-46 |
| MS.gene050381 | MS.gene79114 | -0.813155 | 2.95E-51 | -1.69E-46 |
| MS.gene050504 | MS.gene79114 | 0.85439 | 1.27E-61 | -1.69E-46 |
| MS.gene050518 | MS.gene79114 | 0.85229 | 5.09E-61 | -1.69E-46 |
| MS.gene050538 | MS.gene79114 | 0.864491 | 1.17E-64 | -1.69E-46 |
| MS.gene050577 | MS.gene79114 | 0.861874 | 7.55E-64 | -1.69E-46 |
| MS.gene050593 | MS.gene79114 | 0.835196 | 1.93E-56 | -1.69E-46 |
| MS.gene050612 | MS.gene79114 | 0.826851 | 2.16E-54 | -1.69E-46 |
| MS.gene050647 | MS.gene79114 | -0.824881 | 6.35E-54 | -1.69E-46 |
| MS.gene050670 | MS.gene79114 | 0.82054 | 6.50E-53 | -1.69E-46 |
| MS.gene050699 | MS.gene79114 | 0.82367 | 1.22E-53 | -1.69E-46 |
| MS.gene050747 | MS.gene79114 | 0.904323 | 1.38E-79 | -1.69E-46 |
| MS.gene050772 | MS.gene79114 | 0.85493 | 8.86E-62 | -1.69E-46 |
| MS.gene05080 | MS.gene79114 | 0.849133 | 3.93E-60 | -1.69E-46 |
| MS.gene050812 | MS.gene79114 | 0.808177 | 3.52E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene79114 | 0.828049 | 1.12E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene79114 | 0.828373 | 9.32E-55 | -1.69E-46 |
| MS.gene050950 | MS.gene79114 | 0.893412 | 6.44E-75 | -1.69E-46 |
| MS.gene050986 | MS.gene79114 | 0.801375 | 9.28E-49 | -1.69E-46 |
| MS.gene05100 | MS.gene79114 | 0.848887 | 4.60E-60 | -1.69E-46 |
| MS.gene05105 | MS.gene79114 | 0.823147 | 1.62E-53 | -1.69E-46 |
| MS.gene051167 | MS.gene79114 | -0.801739 | 7.81E-49 | -1.69E-46 |
| MS.gene051387 | MS.gene79114 | 0.859726 | 3.39E-63 | -1.69E-46 |
| MS.gene051551 | MS.gene79114 | 0.834506 | 2.88E-56 | -1.69E-46 |
| MS.gene051666 | MS.gene79114 | 0.889403 | 2.50E-73 | -1.69E-46 |
| MS.gene051694 | MS.gene79114 | 0.819259 | 1.28E-52 | -1.69E-46 |
| MS.gene051734 | MS.gene79114 | 0.812856 | 3.44E-51 | -1.69E-46 |
| MS.gene051838 | MS.gene79114 | 0.814293 | 1.66E-51 | -1.69E-46 |
| MS.gene051858 | MS.gene79114 | 0.80302 | 4.26E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene79114 | 0.859089 | 5.26E-63 | -1.69E-46 |
| MS.gene052414 | MS.gene79114 | 0.802276 | 6.06E-49 | -1.69E-46 |
| MS.gene052421 | MS.gene79114 | 0.841189 | 5.52E-58 | -1.69E-46 |
| MS.gene052481 | MS.gene79114 | 0.802547 | 5.33E-49 | -1.69E-46 |
| MS.gene052482 | MS.gene79114 | 0.848553 | 5.70E-60 | -1.69E-46 |
| MS.gene052486 | MS.gene79114 | 0.847878 | 8.75E-60 | -1.69E-46 |
| MS.gene052501 | MS.gene79114 | 0.844434 | 7.58E-59 | -1.69E-46 |
| MS.gene052503 | MS.gene79114 | 0.854 | 1.65E-61 | -1.69E-46 |
| MS.gene052505 | MS.gene79114 | 0.836443 | 9.33E-57 | -1.69E-46 |
| MS.gene052506 | MS.gene79114 | 0.805813 | 1.11E-49 | -1.69E-46 |
| MS.gene052615 | MS.gene79114 | 0.840596 | 7.90E-58 | -1.69E-46 |
| MS.gene052670 | MS.gene79114 | 0.80572 | 1.17E-49 | -1.69E-46 |
| MS.gene052823 | MS.gene79114 | 0.852176 | 5.48E-61 | -1.69E-46 |
| MS.gene052825 | MS.gene79114 | 0.848226 | 7.01E-60 | -1.69E-46 |
| MS.gene052982 | MS.gene79114 | 0.813559 | 2.41E-51 | -1.69E-46 |
| MS.gene053103 | MS.gene79114 | 0.831987 | 1.22E-55 | -1.69E-46 |
| MS.gene053104 | MS.gene79114 | 0.841409 | 4.83E-58 | -1.69E-46 |
| MS.gene053107 | MS.gene79114 | 0.811389 | 7.18E-51 | -1.69E-46 |
| MS.gene053203 | MS.gene79114 | 0.848777 | 4.94E-60 | -1.69E-46 |
| MS.gene053207 | MS.gene79114 | 0.83313 | 6.37E-56 | -1.69E-46 |
| MS.gene053213 | MS.gene79114 | 0.865405 | 6.05E-65 | -1.69E-46 |
| MS.gene053286 | MS.gene79114 | 0.863024 | 3.34E-64 | -1.69E-46 |
| MS.gene053322 | MS.gene79114 | 0.84246 | 2.55E-58 | -1.69E-46 |
| MS.gene053436 | MS.gene79114 | 0.889151 | 3.13E-73 | -1.69E-46 |
| MS.gene053574 | MS.gene79114 | 0.880212 | 6.61E-70 | -1.69E-46 |
| MS.gene053579 | MS.gene79114 | 0.856751 | 2.60E-62 | -1.69E-46 |
| MS.gene053626 | MS.gene79114 | 0.876393 | 1.45E-68 | -1.69E-46 |
| MS.gene053632 | MS.gene79114 | 0.857699 | 1.37E-62 | -1.69E-46 |
| MS.gene053824 | MS.gene79114 | 0.812673 | 3.77E-51 | -1.69E-46 |
| MS.gene053916 | MS.gene79114 | 0.817831 | 2.69E-52 | -1.69E-46 |
| MS.gene054003 | MS.gene79114 | 0.823952 | 1.05E-53 | -1.69E-46 |
| MS.gene054408 | MS.gene79114 | 0.88161 | 2.08E-70 | -1.69E-46 |
| MS.gene054595 | MS.gene79114 | 0.883658 | 3.72E-71 | -1.69E-46 |
| MS.gene054600 | MS.gene79114 | 0.830291 | 3.19E-55 | -1.69E-46 |
| MS.gene054642 | MS.gene79114 | 0.846958 | 1.57E-59 | -1.69E-46 |
| MS.gene054706 | MS.gene79114 | -0.816221 | 6.19E-52 | -1.69E-46 |
| MS.gene05470 | MS.gene79114 | 0.829074 | 6.31E-55 | -1.69E-46 |
| MS.gene054927 | MS.gene79114 | 0.864524 | 1.14E-64 | -1.69E-46 |
| MS.gene054954 | MS.gene79114 | 0.820369 | 7.11E-53 | -1.69E-46 |
| MS.gene05505 | MS.gene79114 | 0.839195 | 1.83E-57 | -1.69E-46 |
| MS.gene055206 | MS.gene79114 | 0.81066 | 1.03E-50 | -1.69E-46 |
| MS.gene055224 | MS.gene79114 | 0.82672 | 2.32E-54 | -1.69E-46 |
| MS.gene055289 | MS.gene79114 | 0.817552 | 3.11E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene79114 | 0.832093 | 1.15E-55 | -1.69E-46 |
| MS.gene055412 | MS.gene79114 | 0.815466 | 9.11E-52 | -1.69E-46 |
| MS.gene055502 | MS.gene79114 | 0.868983 | 4.35E-66 | -1.69E-46 |
| MS.gene055577 | MS.gene79114 | 0.842788 | 2.09E-58 | -1.69E-46 |
| MS.gene055629 | MS.gene79114 | 0.849326 | 3.48E-60 | -1.69E-46 |
| MS.gene055647 | MS.gene79114 | 0.812419 | 4.28E-51 | -1.69E-46 |
| MS.gene055722 | MS.gene79114 | 0.816905 | 4.35E-52 | -1.69E-46 |
| MS.gene055748 | MS.gene79114 | 0.830823 | 2.36E-55 | -1.69E-46 |
| MS.gene055750 | MS.gene79114 | 0.834774 | 2.47E-56 | -1.69E-46 |
| MS.gene055751 | MS.gene79114 | 0.811278 | 7.58E-51 | -1.69E-46 |
| MS.gene05589 | MS.gene79114 | 0.81505 | 1.13E-51 | -1.69E-46 |
| MS.gene05592 | MS.gene79114 | 0.804397 | 2.20E-49 | -1.69E-46 |
| MS.gene055941 | MS.gene79114 | 0.8311 | 2.02E-55 | -1.69E-46 |
| MS.gene056040 | MS.gene79114 | 0.88696 | 2.17E-72 | -1.69E-46 |
| MS.gene056083 | MS.gene79114 | 0.801418 | 9.09E-49 | -1.69E-46 |
| MS.gene056166 | MS.gene79114 | 0.870219 | 1.72E-66 | -1.69E-46 |
| MS.gene056374 | MS.gene79114 | 0.833741 | 4.48E-56 | -1.69E-46 |
| MS.gene056422 | MS.gene79114 | 0.82729 | 1.70E-54 | -1.69E-46 |
| MS.gene056446 | MS.gene79114 | 0.845398 | 4.16E-59 | -1.69E-46 |
| MS.gene056487 | MS.gene79114 | 0.805311 | 1.42E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene79114 | 0.831226 | 1.88E-55 | -1.69E-46 |
| MS.gene056510 | MS.gene79114 | 0.83806 | 3.60E-57 | -1.69E-46 |
| MS.gene056511 | MS.gene79114 | 0.803635 | 3.17E-49 | -1.69E-46 |
| MS.gene056610 | MS.gene79114 | 0.804343 | 2.26E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene79114 | 0.850309 | 1.85E-60 | -1.69E-46 |
| MS.gene056692 | MS.gene79114 | 0.808411 | 3.14E-50 | -1.69E-46 |
| MS.gene057011 | MS.gene79114 | 0.842072 | 3.23E-58 | -1.69E-46 |
| MS.gene057086 | MS.gene79114 | 0.816667 | 4.91E-52 | -1.69E-46 |
| MS.gene057129 | MS.gene79114 | 0.820748 | 5.82E-53 | -1.69E-46 |
| MS.gene057146 | MS.gene79114 | 0.840973 | 6.30E-58 | -1.69E-46 |
| MS.gene057331 | MS.gene79114 | 0.802248 | 6.14E-49 | -1.69E-46 |
| MS.gene057416 | MS.gene79114 | 0.874161 | 8.38E-68 | -1.69E-46 |
| MS.gene057658 | MS.gene79114 | 0.825783 | 3.88E-54 | -1.69E-46 |
| MS.gene057734 | MS.gene79114 | 0.819697 | 1.01E-52 | -1.69E-46 |
| MS.gene057831 | MS.gene79114 | 0.828972 | 6.68E-55 | -1.69E-46 |
| MS.gene05787 | MS.gene79114 | 0.865804 | 4.53E-65 | -1.69E-46 |
| MS.gene058019 | MS.gene79114 | 0.845379 | 4.21E-59 | -1.69E-46 |
| MS.gene058130 | MS.gene79114 | 0.850595 | 1.53E-60 | -1.69E-46 |
| MS.gene058293 | MS.gene79114 | 0.807332 | 5.32E-50 | -1.69E-46 |
| MS.gene058323 | MS.gene79114 | 0.808403 | 3.15E-50 | -1.69E-46 |
| MS.gene058338 | MS.gene79114 | 0.812199 | 4.78E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene79114 | 0.864258 | 1.38E-64 | -1.69E-46 |
| MS.gene058419 | MS.gene79114 | 0.864645 | 1.05E-64 | -1.69E-46 |
| MS.gene058544 | MS.gene79114 | 0.877188 | 7.68E-69 | -1.69E-46 |
| MS.gene058773 | MS.gene79114 | 0.820046 | 8.43E-53 | -1.69E-46 |
| MS.gene058838 | MS.gene79114 | 0.82022 | 7.70E-53 | -1.69E-46 |
| MS.gene05883 | MS.gene79114 | 0.81606 | 6.72E-52 | -1.69E-46 |
| MS.gene059022 | MS.gene79114 | 0.814239 | 1.70E-51 | -1.69E-46 |
| MS.gene059023 | MS.gene79114 | 0.835733 | 1.41E-56 | -1.69E-46 |
| MS.gene05913 | MS.gene79114 | 0.806381 | 8.46E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene79114 | 0.850017 | 2.23E-60 | -1.69E-46 |
| MS.gene059367 | MS.gene79114 | 0.854504 | 1.18E-61 | -1.69E-46 |
| MS.gene059368 | MS.gene79114 | 0.849253 | 3.64E-60 | -1.69E-46 |
| MS.gene059536 | MS.gene79114 | 0.845403 | 4.15E-59 | -1.69E-46 |
| MS.gene059667 | MS.gene79114 | 0.850052 | 2.18E-60 | -1.69E-46 |
| MS.gene059677 | MS.gene79114 | 0.823367 | 1.44E-53 | -1.69E-46 |
| MS.gene059688 | MS.gene79114 | 0.801188 | 1.01E-48 | -1.69E-46 |
| MS.gene059718 | MS.gene79114 | 0.857922 | 1.17E-62 | -1.69E-46 |
| MS.gene059756 | MS.gene79114 | 0.812172 | 4.85E-51 | -1.69E-46 |
| MS.gene059801 | MS.gene79114 | 0.821369 | 4.19E-53 | -1.69E-46 |
| MS.gene059865 | MS.gene79114 | 0.82275 | 2.00E-53 | -1.69E-46 |
| MS.gene059902 | MS.gene79114 | 0.800488 | 1.41E-48 | -1.69E-46 |
| MS.gene059960 | MS.gene79114 | 0.836165 | 1.10E-56 | -1.69E-46 |
| MS.gene060403 | MS.gene79114 | 0.806103 | 9.68E-50 | -1.69E-46 |
| MS.gene060482 | MS.gene79114 | 0.840348 | 9.18E-58 | -1.69E-46 |
| MS.gene060762 | MS.gene79114 | 0.822123 | 2.80E-53 | -1.69E-46 |
| MS.gene060778 | MS.gene79114 | 0.813963 | 1.96E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene79114 | 0.849953 | 2.32E-60 | -1.69E-46 |
| MS.gene060804 | MS.gene79114 | 0.828128 | 1.07E-54 | -1.69E-46 |
| MS.gene060835 | MS.gene79114 | 0.823609 | 1.26E-53 | -1.69E-46 |
| MS.gene060837 | MS.gene79114 | 0.818551 | 1.85E-52 | -1.69E-46 |
| MS.gene06092 | MS.gene79114 | 0.882389 | 1.09E-70 | -1.69E-46 |
| MS.gene06097 | MS.gene79114 | 0.827649 | 1.39E-54 | -1.69E-46 |
| MS.gene060982 | MS.gene79114 | 0.823276 | 1.51E-53 | -1.69E-46 |
| MS.gene061041 | MS.gene79114 | 0.814554 | 1.45E-51 | -1.69E-46 |
| MS.gene061150 | MS.gene79114 | 0.839584 | 1.45E-57 | -1.69E-46 |
| MS.gene061200 | MS.gene79114 | 0.851359 | 9.34E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79114.t1 | MTR_3g086040 | 81.319 | 182 | 5 | 2 | 1 | 155 | 1 | 180 | 2.40e-99 | 285 |
| MS.gene79114.t1 | MTR_3g086040 | 80.120 | 166 | 4 | 2 | 1 | 139 | 1 | 164 | 3.38e-87 | 253 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79114.t1 | AT1G67700 | 59.358 | 187 | 39 | 5 | 1 | 155 | 1 | 182 | 5.18e-67 | 203 |
| MS.gene79114.t1 | AT1G67700 | 59.358 | 187 | 39 | 5 | 1 | 155 | 1 | 182 | 5.18e-67 | 203 |
| MS.gene79114.t1 | AT1G67700 | 59.358 | 187 | 39 | 5 | 1 | 155 | 1 | 182 | 6.67e-67 | 203 |
| MS.gene79114.t1 | AT1G67700 | 59.358 | 187 | 39 | 5 | 1 | 155 | 1 | 182 | 6.67e-67 | 203 |
| MS.gene79114.t1 | AT1G67700 | 58.824 | 187 | 39 | 6 | 1 | 155 | 1 | 181 | 1.75e-64 | 197 |
Find 34 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGATGTTGGCGTTGAGTTC+TGG | 0.117900 | 3.1:+68885584 | None:intergenic |
| ATTACCGTCGTCTTCAATTT+TGG | 0.126709 | 3.1:+68885470 | None:intergenic |
| TATCCTCTTAGTATTGTTTC+TGG | 0.147550 | 3.1:-68885402 | MS.gene79114:CDS |
| CGAATGAAGACGACGAATTT+AGG | 0.163591 | 3.1:+68885447 | None:intergenic |
| GTATGGCTAATGTTTATCTT+TGG | 0.328706 | 3.1:-68885425 | MS.gene79114:CDS |
| TGGTACCACTGCTAAAATTA+TGG | 0.385812 | 3.1:-68885379 | MS.gene79114:intron |
| TGATGTTGGCGTTGAGTTCT+GGG | 0.389647 | 3.1:+68885585 | None:intergenic |
| GAGTGAGTCGTTGTGGAACC+TGG | 0.423768 | 3.1:+68885629 | None:intergenic |
| TGAGTTCTGGGGAATTGTCT+TGG | 0.431127 | 3.1:+68885597 | None:intergenic |
| GATTATGAATTGCAGGAGAA+TGG | 0.452904 | 3.1:-68884066 | MS.gene79114:intron |
| TCGGCTACTGAATTCAGATA+TGG | 0.454393 | 3.1:-68884190 | MS.gene79114:CDS |
| TTCTACCAGTGATGTTATTG+AGG | 0.459749 | 3.1:-68884025 | None:intergenic |
| CACTATCAAGTTCAAGCTCC+AGG | 0.504458 | 3.1:-68885647 | MS.gene79114:CDS |
| ATTCGTCGTCTTCATTCGTA+TGG | 0.513591 | 3.1:-68885442 | MS.gene79114:CDS |
| TCTGGGGAATTGTCTTGGAA+TGG | 0.516106 | 3.1:+68885602 | None:intergenic |
| CCTCTTAGTATTGTTTCTGG+TGG | 0.528582 | 3.1:-68885399 | MS.gene79114:CDS |
| TGTCTTGGTTGTTGAAGCAA+AGG | 0.532305 | 3.1:-68885547 | MS.gene79114:CDS |
| GTCTTGGTTGTTGAAGCAAA+GGG | 0.544651 | 3.1:-68885546 | MS.gene79114:CDS |
| CCACCAGAAACAATACTAAG+AGG | 0.550315 | 3.1:+68885399 | None:intergenic |
| TCAATTCAAATAGGATGAGA+AGG | 0.553484 | 3.1:-68884254 | MS.gene79114:intron |
| CCAAGACATGGTGGTGGTGG+TGG | 0.560506 | 3.1:+68885562 | None:intergenic |
| ATCTCGAAACGCATGGAAGT+AGG | 0.561046 | 3.1:-68885699 | None:intergenic |
| CTGGAGCTTGAACTTGATAG+TGG | 0.561983 | 3.1:+68885648 | None:intergenic |
| TATCACCTCAATAACATCAC+TGG | 0.580790 | 3.1:+68884020 | None:intergenic |
| CAACCAAGACATGGTGGTGG+TGG | 0.586870 | 3.1:+68885559 | None:intergenic |
| TGGAGAAGAGTGAGTCGTTG+TGG | 0.587691 | 3.1:+68885622 | None:intergenic |
| ACAACACCGTGTACTAAAGT+CGG | 0.619103 | 3.1:-68884209 | MS.gene79114:CDS |
| TTGCTTCAACAACCAAGACA+TGG | 0.623521 | 3.1:+68885550 | None:intergenic |
| GAGCTTGAACTTGATAGTGG+TGG | 0.644572 | 3.1:+68885651 | None:intergenic |
| GATGTTGGCGTTGAGTTCTG+GGG | 0.652469 | 3.1:+68885586 | None:intergenic |
| CAGTAGCCGACTTTAGTACA+CGG | 0.657329 | 3.1:+68884203 | None:intergenic |
| CAACAACCAAGACATGGTGG+TGG | 0.667199 | 3.1:+68885556 | None:intergenic |
| CTTCAACAACCAAGACATGG+TGG | 0.675309 | 3.1:+68885553 | None:intergenic |
| TTACCCAAAATTGAAGACGA+CGG | 0.694339 | 3.1:-68885474 | MS.gene79114:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATATATGTATTGAAATAT+AGG | + | chr3.1:68885570-68885589 | None:intergenic | 10.0% |
| !! | AAATTAATGAAAAAAAAGTT+AGG | + | chr3.1:68884453-68884472 | None:intergenic | 10.0% |
| !! | AATTAATGAAAAAAAAGTTA+GGG | + | chr3.1:68884452-68884471 | None:intergenic | 10.0% |
| !!! | TTTAAATGTTCATAATTTTA+TGG | - | chr3.1:68885070-68885089 | MS.gene79114:intron | 10.0% |
| !! | AAAAAAAAAAATATCGAGAA+TGG | - | chr3.1:68884521-68884540 | MS.gene79114:intron | 15.0% |
| !! | AAAAAAAAAATATCGAGAAT+GGG | - | chr3.1:68884522-68884541 | MS.gene79114:intron | 15.0% |
| !! | ATTAATGAAAAAAAAGTTAG+GGG | + | chr3.1:68884451-68884470 | None:intergenic | 15.0% |
| !! | TATGAACATTTAAAAAATCA+TGG | + | chr3.1:68885064-68885083 | None:intergenic | 15.0% |
| !! | AAAAAAAAATATCGAGAATG+GGG | - | chr3.1:68884523-68884542 | MS.gene79114:intron | 20.0% |
| !! | ACTCTATTAAACTTTCAAAT+TGG | - | chr3.1:68884893-68884912 | MS.gene79114:intron | 20.0% |
| !! | TAGTAAGTGGTTAAAATATA+AGG | + | chr3.1:68884952-68884971 | None:intergenic | 20.0% |
| !! | TCTTGGCAAATTTATTTATA+AGG | - | chr3.1:68884375-68884394 | MS.gene79114:intron | 20.0% |
| !!! | TTTACCTTCAAGATAATTTT+AGG | - | chr3.1:68884758-68884777 | MS.gene79114:intron | 20.0% |
| ! | AAAGTTAAGGACTAATATAG+TGG | + | chr3.1:68884819-68884838 | None:intergenic | 25.0% |
| ! | ATAGACAACGGATAAAATTT+CGG | - | chr3.1:68885244-68885263 | MS.gene79114:intron | 25.0% |
| ! | ATATTAGTCCTTAACTTTGT+AGG | - | chr3.1:68884821-68884840 | MS.gene79114:intron | 25.0% |
| ! | TATTAGTCCTTAACTTTGTA+GGG | - | chr3.1:68884822-68884841 | MS.gene79114:intron | 25.0% |
| !! | AAGATCAAATTGATAGTAAG+TGG | + | chr3.1:68884965-68884984 | None:intergenic | 25.0% |
| !! | TTTTTCTTGGTTATGTATGT+TGG | - | chr3.1:68885396-68885415 | MS.gene79114:CDS | 25.0% |
| !!! | TTTTTCAGATTATGAATTGC+AGG | - | chr3.1:68885640-68885659 | MS.gene79114:CDS | 25.0% |
| AAATGATTCTAACTTGAAGC+CGG | - | chr3.1:68884642-68884661 | MS.gene79114:intron | 30.0% | |
| AAGGTAAAGTCATTCATGTT+TGG | + | chr3.1:68884746-68884765 | None:intergenic | 30.0% | |
| ACACAAATAATCAGCAACAA+TGG | + | chr3.1:68884615-68884634 | None:intergenic | 30.0% | |
| AGAAATTAGAGTCTCCATAA+TGG | + | chr3.1:68885352-68885371 | None:intergenic | 30.0% | |
| ATAGCAAGCAGTTAAATACA+TGG | + | chr3.1:68884920-68884939 | None:intergenic | 30.0% | |
| CACAAATAATCAGCAACAAT+GGG | + | chr3.1:68884614-68884633 | None:intergenic | 30.0% | |
| GAACTACGTGTATGAAAATA+CGG | - | chr3.1:68885184-68885203 | MS.gene79114:intron | 30.0% | |
| GGGACTAAATTCATAATAAG+CGG | + | chr3.1:68884997-68885016 | None:intergenic | 30.0% | |
| GTATGGCTAATGTTTATCTT+TGG | - | chr3.1:68884288-68884307 | MS.gene79114:intron | 30.0% | |
| TATCCTCTTAGTATTGTTTC+TGG | - | chr3.1:68884311-68884330 | MS.gene79114:intron | 30.0% | |
| TCAATTCAAATAGGATGAGA+AGG | - | chr3.1:68885459-68885478 | MS.gene79114:CDS | 30.0% | |
| TCACATTGATGTTAGTCAAA+TGG | + | chr3.1:68885144-68885163 | None:intergenic | 30.0% | |
| TCACTGAATTCATCTTCAAT+TGG | + | chr3.1:68885598-68885617 | None:intergenic | 30.0% | |
| TCTCTTGCCATAATTATAGT+TGG | + | chr3.1:68885217-68885236 | None:intergenic | 30.0% | |
| TGCTGCTAAAGATAATTTCT+TGG | - | chr3.1:68884358-68884377 | MS.gene79114:intron | 30.0% | |
| TGTGTGACCAACTATAATTA+TGG | - | chr3.1:68885207-68885226 | MS.gene79114:intron | 30.0% | |
| TTAGGGGAAAAAATCTTACT+CGG | + | chr3.1:68884435-68884454 | None:intergenic | 30.0% | |
| ! | AAACTTAAAGCTCTAATGTC+CGG | + | chr3.1:68884664-68884683 | None:intergenic | 30.0% |
| ! | ACCTTCAAGATAATTTTAGG+CGG | - | chr3.1:68884761-68884780 | MS.gene79114:intron | 30.0% |
| ! | AGAGACTTTTTGATAGACAA+CGG | - | chr3.1:68885232-68885251 | MS.gene79114:intron | 30.0% |
| ! | TGAGCTCTTTTTAACCATTA+TGG | - | chr3.1:68885335-68885354 | MS.gene79114:intron | 30.0% |
| ! | TTTGAGATATTCACAATCTC+AGG | + | chr3.1:68884863-68884882 | None:intergenic | 30.0% |
| !! | TTAACTGCTTGCTATCAATT+AGG | - | chr3.1:68884925-68884944 | MS.gene79114:intron | 30.0% |
| !!! | ATGATGAGCATGTTTTTTCT+TGG | - | chr3.1:68885383-68885402 | MS.gene79114:CDS | 30.0% |
| AAATTCGTCCCTGACTTTAT+AGG | + | chr3.1:68885110-68885129 | None:intergenic | 35.0% | |
| AATTCGTCCCTGACTTTATA+GGG | + | chr3.1:68885109-68885128 | None:intergenic | 35.0% | |
| ACTTTATAGGGCTCTCAAAT+AGG | + | chr3.1:68885097-68885116 | None:intergenic | 35.0% | |
| CTGCTTGCTTCAATTCAAAT+AGG | - | chr3.1:68885450-68885469 | MS.gene79114:CDS | 35.0% | |
| GATTATGAATTGCAGGAGAA+TGG | - | chr3.1:68885647-68885666 | MS.gene79114:CDS | 35.0% | |
| GTTGAAGCAAAGGGTAAAAA+AGG | - | chr3.1:68884176-68884195 | MS.gene79114:intron | 35.0% | |
| TCCGCCTAAAATTATCTTGA+AGG | + | chr3.1:68884765-68884784 | None:intergenic | 35.0% | |
| TGGTACCACTGCTAAAATTA+TGG | - | chr3.1:68884334-68884353 | MS.gene79114:intron | 35.0% | |
| TTACCCAAAATTGAAGACGA+CGG | - | chr3.1:68884239-68884258 | MS.gene79114:CDS | 35.0% | |
| ! | ATTACCGTCGTCTTCAATTT+TGG | + | chr3.1:68884246-68884265 | None:intergenic | 35.0% |
| ! | TGTATGTTGGTTATGCATGT+TGG | - | chr3.1:68885409-68885428 | MS.gene79114:CDS | 35.0% |
| ! | TTACCGTCGTCTTCAATTTT+GGG | + | chr3.1:68884245-68884264 | None:intergenic | 35.0% |
| !! | AGCAACCATAATTTTAGCAG+TGG | + | chr3.1:68884342-68884361 | None:intergenic | 35.0% |
| !! | TCGTCTTCAATTTTGGGTAA+AGG | + | chr3.1:68884239-68884258 | None:intergenic | 35.0% |
| !! | TCTTCAATTTTGGGTAAAGG+AGG | + | chr3.1:68884236-68884255 | None:intergenic | 35.0% |
| AAAGTCAGGGACGAATTTGA+TGG | - | chr3.1:68885112-68885131 | MS.gene79114:intron | 40.0% | |
| AAGCAGTGAGTAACAAAGAC+AGG | + | chr3.1:68885436-68885455 | None:intergenic | 40.0% | |
| AATCAGCAACAATGGGCATT+TGG | + | chr3.1:68884607-68884626 | None:intergenic | 40.0% | |
| ACAACACCGTGTACTAAAGT+CGG | - | chr3.1:68885504-68885523 | MS.gene79114:CDS | 40.0% | |
| AGTGGTATGATTCTCGCTTA+GGG | + | chr3.1:68884581-68884600 | None:intergenic | 40.0% | |
| ATTCGTCGTCTTCATTCGTA+TGG | - | chr3.1:68884271-68884290 | MS.gene79114:intron | 40.0% | |
| CCACCAGAAACAATACTAAG+AGG | + | chr3.1:68884317-68884336 | None:intergenic | 40.0% | |
| CGAATGAAGACGACGAATTT+AGG | + | chr3.1:68884269-68884288 | None:intergenic | 40.0% | |
| GTCTTGGTTGTTGAAGCAAA+GGG | - | chr3.1:68884167-68884186 | MS.gene79114:intron | 40.0% | |
| TAGTGGTATGATTCTCGCTT+AGG | + | chr3.1:68884582-68884601 | None:intergenic | 40.0% | |
| TCGGCTACTGAATTCAGATA+TGG | - | chr3.1:68885523-68885542 | MS.gene79114:CDS | 40.0% | |
| TGACTAACATCAATGTGACG+TGG | - | chr3.1:68885146-68885165 | MS.gene79114:intron | 40.0% | |
| TGTCTTGGTTGTTGAAGCAA+AGG | - | chr3.1:68884166-68884185 | MS.gene79114:intron | 40.0% | |
| TTGAGAGCCCTATAAAGTCA+GGG | - | chr3.1:68885099-68885118 | MS.gene79114:intron | 40.0% | |
| TTGCTTCAACAACCAAGACA+TGG | + | chr3.1:68884166-68884185 | None:intergenic | 40.0% | |
| TTTGAGAGCCCTATAAAGTC+AGG | - | chr3.1:68885098-68885117 | MS.gene79114:intron | 40.0% | |
| ! | TTTGTAGGGTGCTCTCAAAT+AGG | - | chr3.1:68884836-68884855 | MS.gene79114:intron | 40.0% |
| !! | CCTCTTAGTATTGTTTCTGG+TGG | - | chr3.1:68884314-68884333 | MS.gene79114:intron | 40.0% |
| !! | TCAATTTTGGGTAAAGGAGG+AGG | + | chr3.1:68884233-68884252 | None:intergenic | 40.0% |
| ACAATGGGCATTTGGTCTAG+TGG | + | chr3.1:68884599-68884618 | None:intergenic | 45.0% | |
| AGTGCTTCACAGAGTCAAGA+GGG | + | chr3.1:68885017-68885036 | None:intergenic | 45.0% | |
| CACTATCAAGTTCAAGCTCC+AGG | - | chr3.1:68884066-68884085 | MS.gene79114:intron | 45.0% | |
| CTGGAGCTTGAACTTGATAG+TGG | + | chr3.1:68884068-68884087 | None:intergenic | 45.0% | |
| CTTCAACAACCAAGACATGG+TGG | + | chr3.1:68884163-68884182 | None:intergenic | 45.0% | |
| GCATTCCGAGAATTGAACTC+GGG | - | chr3.1:68884545-68884564 | MS.gene79114:intron | 45.0% | |
| GGCATTCCGAGAATTGAACT+CGG | - | chr3.1:68884544-68884563 | MS.gene79114:intron | 45.0% | |
| TAGTGCTTCACAGAGTCAAG+AGG | + | chr3.1:68885018-68885037 | None:intergenic | 45.0% | |
| TCTGGGGAATTGTCTTGGAA+TGG | + | chr3.1:68884114-68884133 | None:intergenic | 45.0% | |
| ! | CAGTAGCCGACTTTAGTACA+CGG | + | chr3.1:68885513-68885532 | None:intergenic | 45.0% |
| ! | TGATGTTGGCGTTGAGTTCT+GGG | + | chr3.1:68884131-68884150 | None:intergenic | 45.0% |
| !! | ATTTTGGGTAAAGGAGGAGG+AGG | + | chr3.1:68884230-68884249 | None:intergenic | 45.0% |
| !! | GAGAGCACCCTACAAAGTTA+AGG | + | chr3.1:68884832-68884851 | None:intergenic | 45.0% |
| !! | GAGCTTGAACTTGATAGTGG+TGG | + | chr3.1:68884065-68884084 | None:intergenic | 45.0% |
| !! | TGAGTTCTGGGGAATTGTCT+TGG | + | chr3.1:68884119-68884138 | None:intergenic | 45.0% |
| CAACAACCAAGACATGGTGG+TGG | + | chr3.1:68884160-68884179 | None:intergenic | 50.0% | |
| GAGGTCCCGAGTTCAATTCT+CGG | + | chr3.1:68884553-68884572 | None:intergenic | 50.0% | |
| TGGAGAAGAGTGAGTCGTTG+TGG | + | chr3.1:68884094-68884113 | None:intergenic | 50.0% | |
| ! | GATGTTGGCGTTGAGTTCTG+GGG | + | chr3.1:68884130-68884149 | None:intergenic | 50.0% |
| ! | GTGATGTTGGCGTTGAGTTC+TGG | + | chr3.1:68884132-68884151 | None:intergenic | 50.0% |
| ATTCTCGCTTAGGGTGCGAG+AGG | + | chr3.1:68884572-68884591 | None:intergenic | 55.0% | |
| GAGTGAGTCGTTGTGGAACC+TGG | + | chr3.1:68884087-68884106 | None:intergenic | 55.0% | |
| !! | CAACCAAGACATGGTGGTGG+TGG | + | chr3.1:68884157-68884176 | None:intergenic | 55.0% |
| CCACCACCACCACCATGTCT+TGG | - | chr3.1:68884151-68884170 | MS.gene79114:intron | 60.0% | |
| !! | CCAAGACATGGTGGTGGTGG+TGG | + | chr3.1:68884154-68884173 | None:intergenic | 60.0% |
| !! | GGTGGTGGTGGTGGTGATGT+TGG | + | chr3.1:68884145-68884164 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 68884026 | 68885709 | 68884026 | ID=MS.gene79114 |
| chr3.1 | mRNA | 68884026 | 68885709 | 68884026 | ID=MS.gene79114.t1;Parent=MS.gene79114 |
| chr3.1 | exon | 68885380 | 68885709 | 68885380 | ID=MS.gene79114.t1.exon1;Parent=MS.gene79114.t1 |
| chr3.1 | CDS | 68885380 | 68885709 | 68885380 | ID=cds.MS.gene79114.t1;Parent=MS.gene79114.t1 |
| chr3.1 | exon | 68884177 | 68884263 | 68884177 | ID=MS.gene79114.t1.exon2;Parent=MS.gene79114.t1 |
| chr3.1 | CDS | 68884177 | 68884263 | 68884177 | ID=cds.MS.gene79114.t1;Parent=MS.gene79114.t1 |
| chr3.1 | exon | 68884026 | 68884073 | 68884026 | ID=MS.gene79114.t1.exon3;Parent=MS.gene79114.t1 |
| chr3.1 | CDS | 68884026 | 68884073 | 68884026 | ID=cds.MS.gene79114.t1;Parent=MS.gene79114.t1 |
| Gene Sequence |
| Protein sequence |
