AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048993.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048993.01.T01 MTR_2g033490 91.827 208 11 1 9 210 87 294 1.60e-134 379
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048993.01.T01 AT3G17170 71.141 149 43 0 17 165 82 230 2.39e-77 235

Find 39 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGAACTCGTTGATATATT+TGG 0.137864 contig595end:-12500 MsG0080048993.01.T01:intergenic
TTTCTCATTGATATTCAATT+TGG 0.139672 contig595end:+11064 MsG0080048993.01.T01:CDS
CAGTTTCCACGTCAAGCTTT+AGG 0.189454 contig595end:-11293 MsG0080048993.01.T01:intergenic
CATTATAGACGATGATGATT+TGG 0.314854 contig595end:+12717 MsG0080048993.01.T01:CDS
AGTTTCCACGTCAAGCTTTA+GGG 0.316767 contig595end:-11292 MsG0080048993.01.T01:intergenic
GGGAGAAGAAAGGAAGAGTA+TGG 0.343212 contig595end:+12386 MsG0080048993.01.T01:CDS
GCTTGACGTGGAAACTGATA+TGG 0.365705 contig595end:+11299 MsG0080048993.01.T01:CDS
TTCTCATTGATATTCAATTT+GGG 0.368200 contig595end:+11065 MsG0080048993.01.T01:CDS
CAACGAGTTCAAGACTTTGT+TGG 0.383716 contig595end:+12510 MsG0080048993.01.T01:CDS
GGAAGATGGTAAACTTCTTC+CGG 0.390554 contig595end:+11120 MsG0080048993.01.T01:CDS
CTTCGTCCCTCTTTATCACT+AGG 0.396913 contig595end:-12558 MsG0080048993.01.T01:intergenic
AGTATGGAGATTCAGTGATT+GGG 0.412799 contig595end:+12402 MsG0080048993.01.T01:CDS
GAGTATGGAGATTCAGTGAT+TGG 0.428387 contig595end:+12401 MsG0080048993.01.T01:CDS
CAGAAGACAGTTGAGGAAGA+TGG 0.433743 contig595end:+11106 MsG0080048993.01.T01:CDS
GCTGTTAATGAGAAAGTTCA+AGG 0.436751 contig595end:+11871 MsG0080048993.01.T01:CDS
CACAGATGATGAAGAAGAAT+GGG 0.451353 contig595end:+12633 MsG0080048993.01.T01:CDS
GATGATATTGAGGGCGGTGA+TGG 0.471829 contig595end:+12688 MsG0080048993.01.T01:CDS
GTATTAAACTCGGGAGGAGG+AGG 0.481879 contig595end:-12598 MsG0080048993.01.T01:intergenic
GCACAGATGATGAAGAAGAA+TGG 0.500429 contig595end:+12632 MsG0080048993.01.T01:CDS
ATCTGATTATGATGATATTG+AGG 0.526841 contig595end:+12678 MsG0080048993.01.T01:CDS
TTCGACACCTAGTGATAAAG+AGG 0.527803 contig595end:+12551 MsG0080048993.01.T01:CDS
GATGATGAAGATGAGGAAGA+TGG 0.536094 contig595end:+12655 MsG0080048993.01.T01:CDS
TCATCATCATCTTCAAACTC+CGG 0.543493 contig595end:-11139 MsG0080048993.01.T01:intergenic
TCATGAGAAACATGCAGAAG+AGG 0.554875 contig595end:+11843 MsG0080048993.01.T01:CDS
GTATGGAGATTCAGTGATTG+GGG 0.567959 contig595end:+12403 MsG0080048993.01.T01:CDS
AAAGTATTAAACTCGGGAGG+AGG 0.569590 contig595end:-12601 MsG0080048993.01.T01:intergenic
TCTGATTATGATGATATTGA+GGG 0.573289 contig595end:+12679 MsG0080048993.01.T01:CDS
AGAATGGGATGATGAAGATG+AGG 0.580334 contig595end:+12648 MsG0080048993.01.T01:CDS
AGCAACAAAAGTATTAAACT+CGG 0.581584 contig595end:-12608 MsG0080048993.01.T01:intergenic
TGATTGGGGTATGAGAAAGT+TGG 0.581664 contig595end:+12417 MsG0080048993.01.T01:CDS
TCAAGTAAACAATCTCATAG+TGG 0.585631 contig595end:-11819 MsG0080048993.01.T01:intergenic
GCAACAAAAGTATTAAACTC+GGG 0.611767 contig595end:-12607 MsG0080048993.01.T01:intergenic
TGATGATTTGGAAATATCAG+AGG 0.612887 contig595end:+12729 MsG0080048993.01.T01:CDS
TATTAAACTCGGGAGGAGGA+GGG 0.622797 contig595end:-12597 MsG0080048993.01.T01:intergenic
GATGATTTGGAAATATCAGA+GGG 0.644533 contig595end:+12730 MsG0080048993.01.T01:CDS
TGAATCCCTAAAGCTTGACG+TGG 0.671676 contig595end:+11287 MsG0080048993.01.T01:CDS
ACAAAAGTATTAAACTCGGG+AGG 0.675886 contig595end:-12604 MsG0080048993.01.T01:intergenic
TCGACACCTAGTGATAAAGA+GGG 0.685347 contig595end:+12552 MsG0080048993.01.T01:CDS
GATTATGATGATATTGAGGG+CGG 0.728969 contig595end:+12682 MsG0080048993.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCAAATAAAAATTATTAG+AGG - contig595end:11697-11716 MsG0080048993.01.T01:intergenic 10.0%
!!! AAAATGAAAATTTGGAATAA+GGG - contig595end:11250-11269 MsG0080048993.01.T01:intergenic 15.0%
!!! TCAAGTTAAAATGATTTTTT+TGG - contig595end:12284-12303 MsG0080048993.01.T01:intergenic 15.0%
!! AATACAGAACAATAATGATT+TGG - contig595end:12057-12076 MsG0080048993.01.T01:intergenic 20.0%
!! ATAGATTCGAAAAAAATGTA+AGG - contig595end:11921-11940 MsG0080048993.01.T01:intergenic 20.0%
!! ATGTCACTATTGTTAAATAT+AGG + contig595end:12002-12021 MsG0080048993.01.T01:intron 20.0%
!! TAAAAAAGAACCAGAATAAT+CGG - contig595end:12332-12351 MsG0080048993.01.T01:intergenic 20.0%
!! TACTCTTTATTGTATATGTA+TGG + contig595end:11418-11437 MsG0080048993.01.T01:intron 20.0%
!! TTCTCATTGATATTCAATTT+GGG + contig595end:11065-11084 MsG0080048993.01.T01:CDS 20.0%
!! TTTCTCATTGATATTCAATT+TGG + contig595end:11064-11083 MsG0080048993.01.T01:CDS 20.0%
!! TTTGTAAACATTATCTAACA+AGG - contig595end:11495-11514 MsG0080048993.01.T01:intergenic 20.0%
!!! GAAAATGAAAATTTGGAATA+AGG - contig595end:11251-11270 MsG0080048993.01.T01:intergenic 20.0%
!!! TCTGTTTGTTTTTGTTTTTT+TGG + contig595end:11187-11206 MsG0080048993.01.T01:intron 20.0%
!!! TTGATGCAATTTTTATTCAT+AGG + contig595end:11384-11403 MsG0080048993.01.T01:intron 20.0%
! ACTAATGAACTCATTCTATT+TGG - contig595end:11960-11979 MsG0080048993.01.T01:intergenic 25.0%
! ATCTGATTATGATGATATTG+AGG + contig595end:12678-12697 MsG0080048993.01.T01:CDS 25.0%
! ATTATCGTCATTATGGTTTA+GGG - contig595end:11526-11545 MsG0080048993.01.T01:intergenic 25.0%
! TAAACATTATCTAACAAGGT+CGG - contig595end:11491-11510 MsG0080048993.01.T01:intergenic 25.0%
! TCTGATTATGATGATATTGA+GGG + contig595end:12679-12698 MsG0080048993.01.T01:CDS 25.0%
! TTTAATAATAACCTTTGCTG+TGG + contig595end:12193-12212 MsG0080048993.01.T01:intron 25.0%
!!! AGCAACAAAAGTATTAAACT+CGG - contig595end:12611-12630 MsG0080048993.01.T01:intergenic 25.0%
AAACATACTTCCGATTATTC+TGG + contig595end:12319-12338 MsG0080048993.01.T01:intron 30.0%
AATAATTGTATCCACAGCAA+AGG - contig595end:12207-12226 MsG0080048993.01.T01:intergenic 30.0%
CACGTTACTGAATCTTTAAA+GGG - contig595end:11758-11777 MsG0080048993.01.T01:intergenic 30.0%
CAGTAACATGACAAACTATT+TGG + contig595end:11461-11480 MsG0080048993.01.T01:intron 30.0%
CATCTAGATTATCGTCATTA+TGG - contig595end:11533-11552 MsG0080048993.01.T01:intergenic 30.0%
CATTATAGACGATGATGATT+TGG + contig595end:12717-12736 MsG0080048993.01.T01:CDS 30.0%
CCTGTACGAAAATGAAAATT+TGG - contig595end:11258-11277 MsG0080048993.01.T01:intergenic 30.0%
CTGGATTAATGTTATGACTT+TGG + contig595end:11224-11243 MsG0080048993.01.T01:intron 30.0%
CTTGAACTCGTTGATATATT+TGG - contig595end:12503-12522 MsG0080048993.01.T01:intergenic 30.0%
GATTATCGTCATTATGGTTT+AGG - contig595end:11527-11546 MsG0080048993.01.T01:intergenic 30.0%
GGTTAATTGCAATCTTTGAA+AGG - contig595end:11900-11919 MsG0080048993.01.T01:intergenic 30.0%
TCAAGTAAACAATCTCATAG+TGG - contig595end:11822-11841 MsG0080048993.01.T01:intergenic 30.0%
TGTCAAAAGTTAGCAAAAGA+TGG - contig595end:11618-11637 MsG0080048993.01.T01:intergenic 30.0%
! AAACATTTGCAGACTTTTTG+AGG + contig595end:12365-12384 MsG0080048993.01.T01:intron 30.0%
! AACATTTGCAGACTTTTTGA+GGG + contig595end:12366-12385 MsG0080048993.01.T01:intron 30.0%
! CCAAATTTTCATTTTCGTAC+AGG + contig595end:11255-11274 MsG0080048993.01.T01:intron 30.0%
! GATGATTTGGAAATATCAGA+GGG + contig595end:12730-12749 MsG0080048993.01.T01:CDS 30.0%
! TGATGATTTGGAAATATCAG+AGG + contig595end:12729-12748 MsG0080048993.01.T01:CDS 30.0%
! TTTGGTAGTCTAAATAGTTC+TGG + contig595end:11205-11224 MsG0080048993.01.T01:intron 30.0%
!! GCAACAAAAGTATTAAACTC+GGG - contig595end:12610-12629 MsG0080048993.01.T01:intergenic 30.0%
AGTATGGAGATTCAGTGATT+GGG + contig595end:12402-12421 MsG0080048993.01.T01:CDS 35.0%
CACAGATGATGAAGAAGAAT+GGG + contig595end:12633-12652 MsG0080048993.01.T01:CDS 35.0%
GATTATGATGATATTGAGGG+CGG + contig595end:12682-12701 MsG0080048993.01.T01:CDS 35.0%
GCACGTTACTGAATCTTTAA+AGG - contig595end:11759-11778 MsG0080048993.01.T01:intergenic 35.0%
GCTGTTAATGAGAAAGTTCA+AGG + contig595end:11871-11890 MsG0080048993.01.T01:CDS 35.0%
GTCATGAACAAATGACATAG+AGG - contig595end:11666-11685 MsG0080048993.01.T01:intergenic 35.0%
TCAACTGTATTGCCAAATTG+TGG - contig595end:12143-12162 MsG0080048993.01.T01:intergenic 35.0%
TCATCATCATCTTCAAACTC+CGG - contig595end:11142-11161 MsG0080048993.01.T01:intergenic 35.0%
! TCATTTTCAGAAGACAGTTG+AGG + contig595end:11099-11118 MsG0080048993.01.T01:CDS 35.0%
!! ACAAAAGTATTAAACTCGGG+AGG - contig595end:12607-12626 MsG0080048993.01.T01:intergenic 35.0%
!! ATTAGTTTGCCATTTCCATC+TGG + contig595end:11974-11993 MsG0080048993.01.T01:intron 35.0%
!! CATTTTGATGAACTTCGAGA+TGG + contig595end:12474-12493 MsG0080048993.01.T01:CDS 35.0%
AAGATGGATCTAAATCCACC+TGG - contig595end:11602-11621 MsG0080048993.01.T01:intergenic 40.0%
ACATGAATGCCAGATGGAAA+TGG - contig595end:11986-12005 MsG0080048993.01.T01:intergenic 40.0%
AGAATGGGATGATGAAGATG+AGG + contig595end:12648-12667 MsG0080048993.01.T01:CDS 40.0%
AGTTTCCACGTCAAGCTTTA+GGG - contig595end:11295-11314 MsG0080048993.01.T01:intergenic 40.0%
ATAGTGACATGAATGCCAGA+TGG - contig595end:11992-12011 MsG0080048993.01.T01:intergenic 40.0%
ATGTTATAACCGTGCTCTCT+TGG + contig595end:11550-11569 MsG0080048993.01.T01:intron 40.0%
CAACGAGTTCAAGACTTTGT+TGG + contig595end:12510-12529 MsG0080048993.01.T01:CDS 40.0%
GAGTATGGAGATTCAGTGAT+TGG + contig595end:12401-12420 MsG0080048993.01.T01:CDS 40.0%
GATGATGAAGATGAGGAAGA+TGG + contig595end:12655-12674 MsG0080048993.01.T01:CDS 40.0%
GCACAGATGATGAAGAAGAA+TGG + contig595end:12632-12651 MsG0080048993.01.T01:CDS 40.0%
GGAAGATGGTAAACTTCTTC+CGG + contig595end:11120-11139 MsG0080048993.01.T01:CDS 40.0%
GTATGGAGATTCAGTGATTG+GGG + contig595end:12403-12422 MsG0080048993.01.T01:CDS 40.0%
TCATGAGAAACATGCAGAAG+AGG + contig595end:11843-11862 MsG0080048993.01.T01:CDS 40.0%
TCGACACCTAGTGATAAAGA+GGG + contig595end:12552-12571 MsG0080048993.01.T01:CDS 40.0%
TGATTGGGGTATGAGAAAGT+TGG + contig595end:12417-12436 MsG0080048993.01.T01:CDS 40.0%
TTCGACACCTAGTGATAAAG+AGG + contig595end:12551-12570 MsG0080048993.01.T01:CDS 40.0%
! AAAGTATTAAACTCGGGAGG+AGG - contig595end:12604-12623 MsG0080048993.01.T01:intergenic 40.0%
! CACTGTTGCTTACCACAATT+TGG + contig595end:12128-12147 MsG0080048993.01.T01:intron 40.0%
!! GACTTTTTGAGGGAGAAGAA+AGG + contig595end:12376-12395 MsG0080048993.01.T01:intron 40.0%
CAGAAGACAGTTGAGGAAGA+TGG + contig595end:11106-11125 MsG0080048993.01.T01:CDS 45.0%
CAGTTTCCACGTCAAGCTTT+AGG - contig595end:11296-11315 MsG0080048993.01.T01:intergenic 45.0%
CTTATCAGACATTCACCAGG+TGG + contig595end:11584-11603 MsG0080048993.01.T01:intron 45.0%
CTTCGTCCCTCTTTATCACT+AGG - contig595end:12561-12580 MsG0080048993.01.T01:intergenic 45.0%
GCTATAGTGTTATGCCGTAG+CGG + contig595end:12024-12043 MsG0080048993.01.T01:intron 45.0%
GCTTGACGTGGAAACTGATA+TGG + contig595end:11299-11318 MsG0080048993.01.T01:CDS 45.0%
GGGAGAAGAAAGGAAGAGTA+TGG + contig595end:12386-12405 MsG0080048993.01.T01:CDS 45.0%
TATTAAACTCGGGAGGAGGA+GGG - contig595end:12600-12619 MsG0080048993.01.T01:intergenic 45.0%
TGAATCCCTAAAGCTTGACG+TGG + contig595end:11287-11306 MsG0080048993.01.T01:CDS 45.0%
! CATTATGGTTTAGGGCAGCA+TGG - contig595end:11518-11537 MsG0080048993.01.T01:intergenic 45.0%
! GATTTGGTCCAATTCCGCTA+CGG - contig595end:12041-12060 MsG0080048993.01.T01:intergenic 45.0%
! GGTCTTATCAGACATTCACC+AGG + contig595end:11581-11600 MsG0080048993.01.T01:intron 45.0%
GATGATATTGAGGGCGGTGA+TGG + contig595end:12688-12707 MsG0080048993.01.T01:CDS 50.0%
GTATTAAACTCGGGAGGAGG+AGG - contig595end:12601-12620 MsG0080048993.01.T01:intergenic 50.0%
! GTGTTATGCCGTAGCGGAAT+TGG + contig595end:12030-12049 MsG0080048993.01.T01:intron 50.0%
CCAGACTGTCCAAGAGAGCA+CGG - contig595end:11562-11581 MsG0080048993.01.T01:intergenic 55.0%
CGTGCTCTCTTGGACAGTCT+GGG + contig595end:11560-11579 MsG0080048993.01.T01:intron 55.0%
CCGTGCTCTCTTGGACAGTC+TGG + contig595end:11559-11578 MsG0080048993.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
contig595end gene 11058 12768 11058 ID=MsG0080048993.01;Name=MsG0080048993.01
contig595end mRNA 11058 12768 11058 ID=MsG0080048993.01.T01;Parent=MsG0080048993.01;Name=MsG0080048993.01.T01;_AED=0.50;_eAED=0.54;_QI=0|0|0|1|1|1|4|0|210
contig595end exon 11058 11163 11058 ID=MsG0080048993.01.T01:exon:17771;Parent=MsG0080048993.01.T01
contig595end exon 11277 11333 11277 ID=MsG0080048993.01.T01:exon:17772;Parent=MsG0080048993.01.T01
contig595end exon 11815 11892 11815 ID=MsG0080048993.01.T01:exon:17773;Parent=MsG0080048993.01.T01
contig595end exon 12377 12768 12377 ID=MsG0080048993.01.T01:exon:17774;Parent=MsG0080048993.01.T01
contig595end CDS 11058 11163 11058 ID=MsG0080048993.01.T01:cds;Parent=MsG0080048993.01.T01
contig595end CDS 11277 11333 11277 ID=MsG0080048993.01.T01:cds;Parent=MsG0080048993.01.T01
contig595end CDS 11815 11892 11815 ID=MsG0080048993.01.T01:cds;Parent=MsG0080048993.01.T01
contig595end CDS 12377 12768 12377 ID=MsG0080048993.01.T01:cds;Parent=MsG0080048993.01.T01
Gene Sequence

>MsG0080048993.01.T01

ATGAATTTTCTCATTGATATTCAATTTGGGTTTTCAACTTGTCATTTTCAGAAGACAGTTGAGGAAGATGGTAAACTTCTTCCGGAGTTTGAAGATGATGATGAAAGAAAGCTCTATGAATCCCTAAAGCTTGACGTGGAAACTGATATGGACGTTGAACTATTGCGCCACTATGAGATTGTTTACTTGATTCATGAGAAACATGCAGAAGAGGTTGTTGCTGTTAATGAGAAAGTTCAAGACTTTTTGAGGGAGAAGAAAGGAAGAGTATGGAGATTCAGTGATTGGGGTATGAGAAAGTTGGCTTACAAAATACAGAAAGCTAACAACGCACACTACATTTTGATGAACTTCGAGATGGACGCCAAATATATCAACGAGTTCAAGACTTTGTTGGACAAAGATGAAAGAGTTATTCGACACCTAGTGATAAAGAGGGACGAAGCAATCACTGAAGATTGCCCTCCTCCTCCCGAGTTTAATACTTTTGTTGCTAGCACAGATGATGAAGAAGAATGGGATGATGAAGATGAGGAAGATGGATCTGATTATGATGATATTGAGGGCGGTGATGGAATTTTCATTATAGACGATGATGATTTGGAAATATCAGAGGGCCAGAAAATAGCATAA

Protein sequence

>MsG0080048993.01.T01

MNFLIDIQFGFSTCHFQKTVEEDGKLLPEFEDDDERKLYESLKLDVETDMDVELLRHYEIVYLIHEKHAEEVVAVNEKVQDFLREKKGRVWRFSDWGMRKLAYKIQKANNAHYILMNFEMDAKYINEFKTLLDKDERVIRHLVIKRDEAITEDCPPPPEFNTFVASTDDEEEWDDEDEEDGSDYDDIEGGDGIFIIDDDDLEISEGQKIA*