AlfalfaGEDB | MsG0180004736.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004736.01

KEGG: -
GO: -
PFAM: PF03364

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004736.01.T01	XP_013469122.1	94.231	208	9	2	1	208	70	274	1.34E-135	391

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004736.01.T01	A0A072VYK4	94.231	208	9	2	1	208	70	274	6.39e-136	391

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048273.01	MsG0180004736.01	0.800300	1.537354e-48	7.133672e-46
MsG0080048528.01	MsG0180004736.01	0.806770	7.000120e-50	3.831871e-47
MsG0080048820.01	MsG0180004736.01	0.808819	2.567271e-50	1.482584e-47
MsG0080048845.01	MsG0180004736.01	0.834177	3.485399e-56	4.103341e-53
MsG0080048900.01	MsG0180004736.01	0.803158	3.983784e-49	1.987606e-46
MsG0080048969.01	MsG0180004736.01	0.823004	1.749286e-53	1.487908e-50
MsG0080048987.01	MsG0180004736.01	0.807638	4.583829e-50	2.566373e-47
MsG0080048993.01	MsG0180004736.01	0.816876	4.411036e-52	3.163448e-49
MsG0080049070.01	MsG0180004736.01	0.806015	1.009783e-49	5.422270e-47
MsG0180000017.01	MsG0180004736.01	0.853700	2.009130e-61	4.379137e-58

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004736.01.T01	MTR_1g085550	94.231	208	9	2	1	208	70	274	1.62e-139	391
MsG0180004736.01.T01	MTR_8g094800	39.860	143	70	4	33	174	107	234	4.62e-19	85.1
MsG0180004736.01.T01	MTR_8g094800	30.216	139	77	3	26	164	350	468	1.92e-12	65.9
MsG0180004736.01.T01	MTR_8g094800	39.860	143	70	4	33	174	107	234	5.21e-19	85.1
MsG0180004736.01.T01	MTR_8g094800	30.216	139	77	3	26	164	350	468	1.35e-12	66.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004736.01.T01	AT4G01650	59.391	197	73	4	11	203	16	209	1.61e-77	231
MsG0180004736.01.T01	AT4G01650	59.391	197	73	4	11	203	93	286	4.81e-76	231
MsG0180004736.01.T01	AT4G01650	65.854	123	40	1	16	138	100	220	8.11e-54	172
MsG0180004736.01.T01	AT5G08720	37.576	165	86	5	33	196	97	245	1.75e-21	92.4
MsG0180004736.01.T01	AT5G08720	30.935	139	76	3	26	164	328	446	2.00e-11	63.2

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Find 42 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAACCTTAAATCCGTA+AGG	0.508599	1:+81954780	MsG0180004736.01.T01:CDS
AAAGAACCTTAAATCCGTAA+GGG	0.553093	1:+81954781	MsG0180004736.01.T01:CDS
AAGAACCTTAAATCCGTAAG+GGG	0.627039	1:+81954782	MsG0180004736.01.T01:CDS
AATTTACTGAGGGCAAGACC+AGG	0.620856	1:-81952107	None:intergenic
ACAGATTGGAGAGCAGAACT+TGG	0.540322	1:+81953793	MsG0180004736.01.T01:intron
ACGCTTCACTTGATTCCATT+GGG	0.443130	1:+81952045	MsG0180004736.01.T01:CDS
ACGGGCGGCAACCACATCTT+CGG	0.470679	1:-81954722	None:intergenic
AGACTTCAAGATGATTGAAG+GGG	0.615498	1:+81953904	MsG0180004736.01.T01:CDS
AGAGTCGTTGGTTGAAGATG+GGG	0.585019	1:+81951953	MsG0180004736.01.T01:CDS
AGATGTGGTTGCCGCCCGTT+CGG	0.394683	1:+81954726	MsG0180004736.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGAACCTTAAATCCGTA+AGG	+	Chr1:81954780-81954799	MsG0180004736.01.T01:CDS	30.0%
AAAGAACCTTAAATCCGTAA+GGG	+	Chr1:81954781-81954800	MsG0180004736.01.T01:CDS	30.0%
AAATCGAGAAAATAGAGCTA+AGG	+	Chr1:81952328-81952347	MsG0180004736.01.T01:intron	30.0%
ACATACTTCCATGACATATA+TGG	-	Chr1:81953339-81953358	None:intergenic	30.0%
ATAGACTTCAAGATGATTGA+AGG	+	Chr1:81953902-81953921	MsG0180004736.01.T01:CDS	30.0%
ATAGGTCAACATGATTATGT+TGG	+	Chr1:81953176-81953195	MsG0180004736.01.T01:intron	30.0%
ATCCAGAAGAATTCATAAGA+GGG	-	Chr1:81953986-81954005	None:intergenic	30.0%
ATGATTATATGCCATGAAGT+AGG	-	Chr1:81953282-81953301	None:intergenic	30.0%
CTCAGTAAATTGATCCAAAA+AGG	+	Chr1:81952119-81952138	MsG0180004736.01.T01:CDS	30.0%
GATATAAAAGTAGTAGGAAG+GGG	+	Chr1:81952673-81952692	MsG0180004736.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	81951921	81954856	81951921	ID=MsG0180004736.01;Name=MsG0180004736.01
Chr1	mRNA	81951921	81954856	81951921	ID=MsG0180004736.01.T01;Parent=MsG0180004736.01;Name=MsG0180004736.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|1\|1\|3\|0\|208
Chr1	exon	81951921	81952166	81951921	ID=MsG0180004736.01.T01:exon:34553;Parent=MsG0180004736.01.T01
Chr1	exon	81953797	81953964	81953797	ID=MsG0180004736.01.T01:exon:34554;Parent=MsG0180004736.01.T01
Chr1	exon	81954644	81954856	81954644	ID=MsG0180004736.01.T01:exon:34555;Parent=MsG0180004736.01.T01
Chr1	CDS	81951921	81952166	81951921	ID=MsG0180004736.01.T01:cds;Parent=MsG0180004736.01.T01
Chr1	CDS	81953797	81953964	81953797	ID=MsG0180004736.01.T01:cds;Parent=MsG0180004736.01.T01
Chr1	CDS	81954644	81954856	81954644	ID=MsG0180004736.01.T01:cds;Parent=MsG0180004736.01.T01

Gene Sequence

>MsG0180004736.01.T01

TTGGTGACAGAGGATGATGAAGTTGAAGATTCAGAGTCGTTGGTTGAAGATGGGGTGTACATAGAAGTCACGAAGCTTGAGAATGAGAAGAATTCACGCAGAATTGAGTCTAGGATTTCTATCGACGCTTCACTTGATTCCATTGGGAATATCTTGACTGATTATGAAAGGTTGGCTGATTTCATTCCTGGTCTTGCCCTCAGTAAATTGATCCAAAAAGGTCCCAATTTTGCTCGACTCTTGCAGATTGGAGAGCAGAACTTGGCATTTGGGTTAAAATTTGATGCTAAAGGAGTTATAGATTGTTTTGAGAAAGAATTGGAGACTCTTCCATCTGGTATGAAGCGTGACATAGACTTCAAGATGATTGAAGGGGATTTTCAGCTCTTCGAAGGAAAATGGTCTATTTTGCAGACTTTCAGCGATGGAAGTTGTGAGGACTCTCCGGTTCGAGAAATTAACACGACTCTTTCATACATTGTTGATGTTAAGCCGAAGATGTGGTTGCCGCCCGTTCGGCTGATAGAGGGTAGACTTTGCAATGAGATAAAAAAGAACCTTAAATCCGTAAGGGGTGAAGCTCAGAAAGCTACTGACAGAACTGTGAATGCAAATCAATTTGAATGA

Protein sequence

>MsG0180004736.01.T01

LVTEDDEVEDSESLVEDGVYIEVTKLENEKNSRRIESRISIDASLDSIGNILTDYERLADFIPGLALSKLIQKGPNFARLLQIGEQNLAFGLKFDAKGVIDCFEKELETLPSGMKRDIDFKMIEGDFQLFEGKWSILQTFSDGSCEDSPVREINTTLSYIVDVKPKMWLPPVRLIEGRLCNEIKKNLKSVRGEAQKATDRTVNANQFE*