Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0316020 | XP_013462742.2 | 95.714 | 280 | 12 | 0 | 1 | 280 | 1 | 280 | 0.0 | 539 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0316020 | sp|P0DO21|GIP2_NICBE | 36.940 | 268 | 140 | 6 | 30 | 280 | 37 | 292 | 4.68e-40 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0013270 | Msa0316020 | 0.848747 | 5.032590e-60 | -8.615850e-47 |
| Msa0017710 | Msa0316020 | 0.819795 | 9.627127e-53 | -8.615850e-47 |
| Msa0025570 | Msa0316020 | 0.807254 | 5.529504e-50 | -8.615850e-47 |
| Msa0036810 | Msa0316020 | 0.817819 | 2.706220e-52 | -8.615850e-47 |
| Msa0037180 | Msa0316020 | 0.807036 | 6.150530e-50 | -8.615850e-47 |
| Msa0045810 | Msa0316020 | 0.810147 | 1.331870e-50 | -8.615850e-47 |
| Msa0046200 | Msa0316020 | 0.804175 | 2.450065e-49 | -8.615850e-47 |
| Msa0046970 | Msa0316020 | 0.817535 | 3.135988e-52 | -8.615850e-47 |
| Msa0062010 | Msa0316020 | 0.838948 | 2.122487e-57 | -8.615850e-47 |
| Msa0062190 | Msa0316020 | 0.812171 | 4.848187e-51 | -8.615850e-47 |
| Msa0063540 | Msa0316020 | 0.838068 | 3.581846e-57 | -8.615850e-47 |
| Msa0081030 | Msa0316020 | 0.814702 | 1.346626e-51 | -8.615850e-47 |
| Msa0106540 | Msa0316020 | 0.838709 | 2.448034e-57 | -8.615850e-47 |
| Msa0108350 | Msa0316020 | 0.833617 | 4.810667e-56 | -8.615850e-47 |
| Msa0121320 | Msa0316020 | 0.809741 | 1.628994e-50 | -8.615850e-47 |
| Msa0139500 | Msa0316020 | 0.800809 | 1.211023e-48 | -8.615850e-47 |
| Msa0160380 | Msa0316020 | 0.818408 | 1.990644e-52 | -8.615850e-47 |
| Msa0166180 | Msa0316020 | 0.810793 | 9.657715e-51 | -8.615850e-47 |
| Msa0177320 | Msa0316020 | 0.814882 | 1.228272e-51 | -8.615850e-47 |
| Msa0180210 | Msa0316020 | 0.818739 | 1.674862e-52 | -8.615850e-47 |
| Msa0190890 | Msa0316020 | 0.809534 | 1.804685e-50 | -8.615850e-47 |
| Msa0196250 | Msa0316020 | 0.805497 | 1.297654e-49 | -8.615850e-47 |
| Msa0196260 | Msa0316020 | 0.834685 | 2.597430e-56 | -8.615850e-47 |
| Msa0211460 | Msa0316020 | 0.800017 | 1.755391e-48 | -8.615850e-47 |
| Msa0233210 | Msa0316020 | 0.800218 | 1.598050e-48 | -8.615850e-47 |
| Msa0238110 | Msa0316020 | 0.863466 | 2.440192e-64 | -8.615850e-47 |
| Msa0258010 | Msa0316020 | 0.809413 | 1.915610e-50 | -8.615850e-47 |
| Msa0277520 | Msa0316020 | 0.802337 | 5.884425e-49 | -8.615850e-47 |
| Msa0279000 | Msa0316020 | 0.800554 | 1.364934e-48 | -8.615850e-47 |
| Msa0279810 | Msa0316020 | 0.805341 | 1.398866e-49 | -8.615850e-47 |
| Msa0299030 | Msa0316020 | 0.806553 | 7.780596e-50 | -8.615850e-47 |
| Msa0307630 | Msa0316020 | 0.813201 | 2.884999e-51 | -8.615850e-47 |
| Msa0316000 | Msa0316020 | 0.847923 | 8.504940e-60 | -8.615850e-47 |
| Msa0316020 | Msa0319220 | 0.812856 | 3.434271e-51 | -8.615850e-47 |
| Msa0316020 | Msa0328870 | 0.801002 | 1.105886e-48 | -8.615850e-47 |
| Msa0316020 | Msa0349100 | 0.812036 | 5.188379e-51 | -8.615850e-47 |
| Msa0316020 | Msa0349910 | 0.805523 | 1.281031e-49 | -8.615850e-47 |
| Msa0316020 | Msa0349930 | 0.822636 | 2.131090e-53 | -8.615850e-47 |
| Msa0316020 | Msa0354600 | 0.808888 | 2.482134e-50 | -8.615850e-47 |
| Msa0316020 | Msa0379480 | 0.810284 | 1.244082e-50 | -8.615850e-47 |
| Msa0316020 | Msa0386880 | 0.800737 | 1.252506e-48 | -8.615850e-47 |
| Msa0316020 | Msa0386910 | 0.818970 | 1.483961e-52 | -8.615850e-47 |
| Msa0316020 | Msa0419570 | 0.816757 | 4.691577e-52 | -8.615850e-47 |
| Msa0316020 | Msa0444210 | 0.807566 | 4.748327e-50 | -8.615850e-47 |
| Msa0316020 | Msa0457070 | -0.805316 | 1.415784e-49 | -8.615850e-47 |
| Msa0316020 | Msa0467170 | 0.812250 | 4.658831e-51 | -8.615850e-47 |
| Msa0316020 | Msa0467330 | 0.809197 | 2.130956e-50 | -8.615850e-47 |
| Msa0316020 | Msa0475330 | 0.816554 | 5.209120e-52 | -8.615850e-47 |
| Msa0316020 | Msa0508560 | 0.804020 | 2.639821e-49 | -8.615850e-47 |
| Msa0316020 | Msa0523720 | 0.806527 | 7.876839e-50 | -8.615850e-47 |
| Msa0316020 | Msa0532600 | 0.805306 | 1.422873e-49 | -8.615850e-47 |
| Msa0316020 | Msa0555280 | 0.815491 | 8.997381e-52 | -8.615850e-47 |
| Msa0316020 | Msa0565700 | 0.828449 | 8.933665e-55 | -8.615850e-47 |
| Msa0316020 | Msa0567330 | 0.818030 | 2.424725e-52 | -8.615850e-47 |
| Msa0316020 | Msa0567570 | 0.819325 | 1.232227e-52 | -8.615850e-47 |
| Msa0316020 | Msa0576320 | 0.814105 | 1.825086e-51 | -8.615850e-47 |
| Msa0316020 | Msa0584370 | 0.801631 | 8.221162e-49 | -8.615850e-47 |
| Msa0316020 | Msa0608820 | 0.824964 | 6.066193e-54 | -8.615850e-47 |
| Msa0316020 | Msa0610700 | -0.807521 | 4.853154e-50 | -8.615850e-47 |
| Msa0316020 | Msa0614960 | 0.821965 | 3.049444e-53 | -8.615850e-47 |
| Msa0316020 | Msa0618680 | 0.810825 | 9.509294e-51 | -8.615850e-47 |
| Msa0316020 | Msa0629160 | 0.820596 | 6.308878e-53 | -8.615850e-47 |
| Msa0316020 | Msa0655370 | 0.807955 | 3.925141e-50 | -8.615850e-47 |
| Msa0316020 | Msa0671400 | 0.807779 | 4.278638e-50 | -8.615850e-47 |
| Msa0316020 | Msa0686830 | 0.800852 | 1.186787e-48 | -8.615850e-47 |
| Msa0316020 | Msa0687220 | 0.808877 | 2.495191e-50 | -8.615850e-47 |
| Msa0316020 | Msa0691610 | 0.820072 | 8.322007e-53 | -8.615850e-47 |
| Msa0316020 | Msa0699170 | 0.827375 | 1.619685e-54 | -8.615850e-47 |
| Msa0316020 | Msa0732970 | 0.815834 | 7.545322e-52 | -8.615850e-47 |
| Msa0316020 | Msa0738680 | 0.815061 | 1.120985e-51 | -8.615850e-47 |
| Msa0316020 | Msa0739720 | 0.800514 | 1.390582e-48 | -8.615850e-47 |
| Msa0316020 | Msa0745140 | 0.811666 | 6.244864e-51 | -8.615850e-47 |
| Msa0316020 | Msa0773960 | 0.826051 | 3.354338e-54 | -8.615850e-47 |
| Msa0316020 | Msa0776640 | 0.811012 | 8.662715e-51 | -8.615850e-47 |
| Msa0316020 | Msa0789220 | 0.804661 | 1.940934e-49 | -8.615850e-47 |
| Msa0316020 | Msa0792410 | 0.810999 | 8.717685e-51 | -8.615850e-47 |
| Msa0316020 | Msa0802600 | 0.808241 | 3.412315e-50 | -8.615850e-47 |
| Msa0316020 | Msa0804170 | 0.838625 | 2.572838e-57 | -8.615850e-47 |
| Msa0316020 | Msa0819150 | 0.814427 | 1.549461e-51 | -8.615850e-47 |
| Msa0316020 | Msa0825920 | 0.817404 | 3.356057e-52 | -8.615850e-47 |
| Msa0316020 | Msa0831890 | 0.801855 | 7.395559e-49 | -8.615850e-47 |
| Msa0316020 | Msa0841520 | 0.827922 | 1.196915e-54 | -8.615850e-47 |
| Msa0316020 | Msa0859270 | 0.804418 | 2.180848e-49 | -8.615850e-47 |
| Msa0316020 | Msa0869660 | 0.813912 | 2.013114e-51 | -8.615850e-47 |
| Msa0316020 | Msa0880910 | 0.814487 | 1.502458e-51 | -8.615850e-47 |
| Msa0316020 | Msa0903020 | 0.834890 | 2.307227e-56 | -8.615850e-47 |
| Msa0316020 | Msa0932140 | 0.828442 | 8.971901e-55 | -8.615850e-47 |
| Msa0316020 | Msa0932150 | 0.840036 | 1.107381e-57 | -8.615850e-47 |
| Msa0316020 | Msa0967780 | 0.807675 | 4.500800e-50 | -8.615850e-47 |
| Msa0316020 | Msa0970270 | 0.828550 | 8.445739e-55 | -8.615850e-47 |
| Msa0316020 | Msa1029860 | 0.814633 | 1.394819e-51 | -8.615850e-47 |
| Msa0316020 | Msa1055450 | 0.818548 | 1.850435e-52 | -8.615850e-47 |
| Msa0316020 | Msa1066150 | 0.808598 | 2.863108e-50 | -8.615850e-47 |
| Msa0316020 | Msa1068710 | 0.822395 | 2.424073e-53 | -8.615850e-47 |
| Msa0316020 | Msa1068730 | 0.826562 | 2.533947e-54 | -8.615850e-47 |
| Msa0316020 | Msa1068940 | 0.800783 | 1.225753e-48 | -8.615850e-47 |
| Msa0316020 | Msa1103450 | 0.800918 | 1.150451e-48 | -8.615850e-47 |
| Msa0316020 | Msa1120960 | 0.812725 | 3.668576e-51 | -8.615850e-47 |
| Msa0316020 | Msa1147010 | 0.813070 | 3.083165e-51 | -8.615850e-47 |
| Msa0316020 | Msa1156730 | 0.812738 | 3.644847e-51 | -8.615850e-47 |
| Msa0316020 | Msa1157210 | 0.801221 | 9.974488e-49 | -8.615850e-47 |
| Msa0316020 | Msa1165460 | 0.813728 | 2.209405e-51 | -8.615850e-47 |
| Msa0316020 | Msa1179180 | 0.805821 | 1.109559e-49 | -8.615850e-47 |
| Msa0316020 | Msa1191640 | 0.801265 | 9.767496e-49 | -8.615850e-47 |
| Msa0316020 | Msa1205270 | -0.803617 | 3.200400e-49 | -8.615850e-47 |
| Msa0316020 | Msa1223540 | 0.818774 | 1.644508e-52 | -8.615850e-47 |
| Msa0316020 | Msa1223620 | 0.809578 | 1.765754e-50 | -8.615850e-47 |
| Msa0316020 | Msa1223960 | 0.819164 | 1.340709e-52 | -8.615850e-47 |
| Msa0316020 | Msa1223990 | 0.812550 | 4.007345e-51 | -8.615850e-47 |
| Msa0316020 | Msa1236860 | -0.800399 | 1.467655e-48 | -8.615850e-47 |
| Msa0316020 | Msa1263110 | 0.812162 | 4.871653e-51 | -8.615850e-47 |
| Msa0316020 | Msa1263120 | 0.805057 | 1.604436e-49 | -8.615850e-47 |
| Msa0316020 | Msa1263520 | 0.803318 | 3.690757e-49 | -8.615850e-47 |
| Msa0316020 | Msa1273320 | 0.813277 | 2.776878e-51 | -8.615850e-47 |
| Msa0316020 | Msa1281780 | 0.802406 | 5.697215e-49 | -8.615850e-47 |
| Msa0316020 | Msa1297220 | -0.815876 | 7.384010e-52 | -8.615850e-47 |
| Msa0316020 | Msa1311370 | 0.814809 | 1.274839e-51 | -8.615850e-47 |
| Msa0316020 | Msa1314030 | 0.804232 | 2.384910e-49 | -8.615850e-47 |
| Msa0316020 | Msa1342760 | 0.834764 | 2.482066e-56 | -8.615850e-47 |
| Msa0316020 | Msa1343450 | -0.805818 | 1.110903e-49 | -8.615850e-47 |
| Msa0316020 | Msa1361980 | 0.812646 | 3.817673e-51 | -8.615850e-47 |
| Msa0316020 | Msa1368420 | 0.823904 | 1.077537e-53 | -8.615850e-47 |
| Msa0316020 | Msa1383220 | 0.825733 | 3.991352e-54 | -8.615850e-47 |
| Msa0316020 | Msa1396100 | 0.800108 | 1.681974e-48 | -8.615850e-47 |
| Msa0316020 | Msa1400300 | 0.800565 | 1.357852e-48 | -8.615850e-47 |
| Msa0316020 | Msa1405830 | 0.837840 | 4.098436e-57 | -8.615850e-47 |
| Msa0316020 | Msa1407810 | 0.817449 | 3.278565e-52 | -8.615850e-47 |
| Msa0316020 | Msa1413020 | 0.833968 | 3.932008e-56 | -8.615850e-47 |
| Msa0316020 | Msa1437780 | 0.815795 | 7.699872e-52 | -8.615850e-47 |
| Msa0316020 | Msa1455360 | 0.832979 | 6.939365e-56 | -8.615850e-47 |
| Msa0316020 | Msa1460520 | 0.823958 | 1.046493e-53 | -8.615850e-47 |
| Msa0316020 | Msa1462610 | 0.807422 | 5.095227e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0316020 | MtrunA17_Chr2g0287781 | 95.714 | 280 | 12 | 0 | 1 | 280 | 924 | 1203 | 0.0 | 539 |
| Msa0316020 | MtrunA17_Chr2g0287781 | 94.643 | 280 | 15 | 0 | 1 | 280 | 462 | 741 | 0.0 | 534 |
| Msa0316020 | MtrunA17_Chr2g0287781 | 74.035 | 285 | 60 | 8 | 1 | 280 | 1 | 276 | 2.13e-125 | 387 |
| Msa0316020 | MtrunA17_Chr2g0287811 | 83.274 | 281 | 46 | 1 | 1 | 280 | 1 | 281 | 1.62e-167 | 470 |
| Msa0316020 | MtrunA17_Chr2g0287841 | 52.692 | 260 | 111 | 7 | 27 | 280 | 55 | 308 | 5.56e-79 | 246 |
| Msa0316020 | MtrunA17_Chr2g0287861 | 52.290 | 262 | 113 | 7 | 25 | 280 | 29 | 284 | 4.47e-78 | 243 |
| Msa0316020 | MtrunA17_Chr4g0065581 | 44.043 | 277 | 124 | 11 | 27 | 280 | 37 | 305 | 8.06e-54 | 181 |
| Msa0316020 | MtrunA17_Chr1g0185661 | 40.068 | 292 | 138 | 10 | 13 | 280 | 661 | 939 | 2.74e-43 | 157 |
| Msa0316020 | MtrunA17_Chr1g0185661 | 39.080 | 261 | 130 | 9 | 30 | 275 | 36 | 282 | 1.81e-36 | 137 |
| Msa0316020 | MtrunA17_Chr1g0185651 | 38.182 | 275 | 139 | 7 | 25 | 280 | 41 | 303 | 4.28e-42 | 150 |
| Msa0316020 | MtrunA17_Chr1g0187841 | 34.028 | 288 | 150 | 10 | 8 | 280 | 13 | 275 | 1.23e-28 | 113 |
| Msa0316020 | MtrunA17_Chr2g0287821 | 32.836 | 268 | 148 | 10 | 30 | 280 | 59 | 311 | 5.15e-28 | 112 |
| Msa0316020 | MtrunA17_Chr7g0273041 | 33.161 | 193 | 100 | 7 | 30 | 207 | 41 | 219 | 8.91e-24 | 99.8 |
| Msa0316020 | MtrunA17_Chr2g0287831 | 26.962 | 293 | 170 | 10 | 1 | 279 | 1 | 263 | 2.44e-11 | 63.5 |
| Msa0316020 | MtrunA17_Chr3g0130931 | 27.273 | 275 | 141 | 14 | 24 | 280 | 91 | 324 | 3.57e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0316020 | AT1G03220.1 | 35.354 | 297 | 151 | 10 | 1 | 275 | 1 | 278 | 2.81e-36 | 134 |
| Msa0316020 | AT1G03230.1 | 33.977 | 259 | 144 | 7 | 30 | 275 | 35 | 279 | 1.29e-32 | 124 |
| Msa0316020 | AT5G19110.1 | 30.545 | 275 | 153 | 10 | 7 | 273 | 10 | 254 | 2.10e-21 | 93.2 |
| Msa0316020 | AT5G19110.2 | 30.545 | 275 | 153 | 10 | 7 | 273 | 46 | 290 | 2.39e-21 | 93.6 |
| Msa0316020 | AT5G19100.1 | 27.755 | 245 | 149 | 9 | 25 | 265 | 28 | 248 | 4.06e-14 | 72.0 |
Find 46 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCATCATTAGTGCAAATTT+TGG | 0.157773 | 2_4:-12801842 | None:intergenic |
| GAAGTGCTATTGTGAGAATT+AGG | 0.193439 | 2_4:-12801618 | None:intergenic |
| GGGTAGGATCTTCTCTTTCC+AGG | 0.247339 | 2_4:+12802235 | Msa0316020:CDS |
| AACTTGTTAAGAAGTTTAAA+AGG | 0.299717 | 2_4:-12802162 | None:intergenic |
| GGAGCTAATGAAAGCTGTTT+TGG | 0.299919 | 2_4:-12802141 | None:intergenic |
| ATTGTAGGTTGAAGAGTTGT+AGG | 0.302927 | 2_4:-12801803 | None:intergenic |
| AAAACTAGTAAAGGTATATT+AGG | 0.307799 | 2_4:+12802093 | Msa0316020:CDS |
| GCATCACCTTCGGTAAATAT+TGG | 0.361876 | 2_4:-12802306 | None:intergenic |
| ATCTATTCTCCACTTTACTT+TGG | 0.363101 | 2_4:+12801701 | Msa0316020:CDS |
| ACCATTTCCCTTATTGTCAA+TGG | 0.371284 | 2_4:-12802406 | None:intergenic |
| CCTTATTGTCAATGGACAAA+AGG | 0.380235 | 2_4:-12802398 | None:intergenic |
| TCTATTCTCCACTTTACTTT+GGG | 0.380236 | 2_4:+12801702 | Msa0316020:CDS |
| TCCATTGACAATAAGGGAAA+TGG | 0.384261 | 2_4:+12802405 | Msa0316020:CDS |
| AAGTGCTATTGTGAGAATTA+GGG | 0.386624 | 2_4:-12801617 | None:intergenic |
| CTAAAGGGGTTAATAATAAG+AGG | 0.451517 | 2_4:-12802276 | None:intergenic |
| CTTATTGTCAATGGACAAAA+GGG | 0.453185 | 2_4:-12802397 | None:intergenic |
| GTGGAGAATAGATTGGTTGC+TGG | 0.456001 | 2_4:-12801691 | None:intergenic |
| AAAGTCTCTGACTTTATCTC+TGG | 0.458442 | 2_4:+12802006 | Msa0316020:CDS |
| AATATTGGAGCAGTGCTAAA+GGG | 0.461586 | 2_4:-12802291 | None:intergenic |
| TTCACAAGAGATGGGATTGT+AGG | 0.463458 | 2_4:-12801818 | None:intergenic |
| TGCAATGGCCCTTTCAAACC+AGG | 0.466930 | 2_4:+12801877 | Msa0316020:CDS |
| AAAATTTGCACTAATGATGC+TGG | 0.488856 | 2_4:+12801844 | Msa0316020:CDS |
| TTTGAGCAACCTGGTTTGAA+AGG | 0.494581 | 2_4:-12801886 | None:intergenic |
| TTGCAGTCATACCATAGGAT+TGG | 0.501466 | 2_4:-12801775 | None:intergenic |
| AATAGTAGTGAGTTGAAACC+TGG | 0.501895 | 2_4:-12802253 | None:intergenic |
| CAAGTATTGTTTGAGCAACC+TGG | 0.502875 | 2_4:-12801895 | None:intergenic |
| AAATATTGGAGCAGTGCTAA+AGG | 0.506396 | 2_4:-12802292 | None:intergenic |
| CTACCAAGTTGGTTTGAAGA+TGG | 0.508156 | 2_4:-12802198 | None:intergenic |
| TTGAGCAACCTGGTTTGAAA+GGG | 0.516540 | 2_4:-12801885 | None:intergenic |
| TGTTCCTACCCAAAGTAAAG+TGG | 0.518880 | 2_4:-12801710 | None:intergenic |
| GATGTGAAATCAATTAAAGT+TGG | 0.521003 | 2_4:+12802348 | Msa0316020:CDS |
| TGGAGAATAGATTGGTTGCT+GGG | 0.521690 | 2_4:-12801690 | None:intergenic |
| TGCTATTGTGAGAATTAGGG+AGG | 0.528613 | 2_4:-12801614 | None:intergenic |
| TTCATAAGAAGCATCACCTT+CGG | 0.531554 | 2_4:-12802316 | None:intergenic |
| ACAAAATTGAATTCATGTGT+AGG | 0.537540 | 2_4:-12801736 | None:intergenic |
| CAATGAAAAGACTACCAAGT+TGG | 0.546167 | 2_4:-12802209 | None:intergenic |
| AAGTAAAGTGGAGAATAGAT+TGG | 0.556509 | 2_4:-12801698 | None:intergenic |
| GCTGGATGCACTAGCTGCAA+TGG | 0.559285 | 2_4:+12801862 | Msa0316020:CDS |
| ATATTGGAGGACCAATCCTA+TGG | 0.571849 | 2_4:+12801764 | Msa0316020:CDS |
| ATGAAAGTGGTGTTCTTGCA+AGG | 0.573514 | 2_4:-12802116 | None:intergenic |
| GTGAAATCAATTAAAGTTGG+TGG | 0.587363 | 2_4:+12802351 | Msa0316020:CDS |
| TTACCATCTTCAAACCAACT+TGG | 0.596389 | 2_4:+12802195 | Msa0316020:CDS |
| TTCTCCACTTTACTTTGGGT+AGG | 0.615543 | 2_4:+12801706 | Msa0316020:CDS |
| ATATTGGAGCAGTGCTAAAG+GGG | 0.623612 | 2_4:-12802290 | None:intergenic |
| ACTGCTCCAATATTTACCGA+AGG | 0.681995 | 2_4:+12802300 | Msa0316020:CDS |
| GGTTGCTCAAACAATACTTG+TGG | 0.698580 | 2_4:+12801898 | Msa0316020:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCAATTTTGTGATTGATAT+TGG | + | chr2_4:12801748-12801767 | Msa0316020:CDS | 20.0% |
| !!! | AATATACCTTTACTAGTTTT+TGG | - | chr2_4:12802093-12802112 | None:intergenic | 20.0% |
| !!! | AAAACTAGTAAAGGTATATT+AGG | + | chr2_4:12802093-12802112 | Msa0316020:CDS | 20.0% |
| !!! | AACTTGTTAAGAAGTTTAAA+AGG | - | chr2_4:12802165-12802184 | None:intergenic | 20.0% |
| !!! | TTCAATTTTGTGATTGATAT+TGG | + | chr2_4:12801748-12801767 | Msa0316020:CDS | 20.0% |
| !!! | AATATACCTTTACTAGTTTT+TGG | - | chr2_4:12802093-12802112 | None:intergenic | 20.0% |
| !!! | AAAACTAGTAAAGGTATATT+AGG | + | chr2_4:12802093-12802112 | Msa0316020:CDS | 20.0% |
| !!! | AACTTGTTAAGAAGTTTAAA+AGG | - | chr2_4:12802165-12802184 | None:intergenic | 20.0% |
| ! | ACAAAATTGAATTCATGTGT+AGG | - | chr2_4:12801739-12801758 | None:intergenic | 25.0% |
| !! | AATTTTGTGATTGATATTGG+AGG | + | chr2_4:12801751-12801770 | Msa0316020:CDS | 25.0% |
| ! | GAAAAAATAGCATCAACTAA+TGG | - | chr2_4:12801940-12801959 | None:intergenic | 25.0% |
| !!! | TTAGTTGATGCTATTTTTTC+AGG | + | chr2_4:12801940-12801959 | Msa0316020:CDS | 25.0% |
| !!! | GAGACTTTGATTTTAGATTT+TGG | - | chr2_4:12801994-12802013 | None:intergenic | 25.0% |
| ! | GATGTGAAATCAATTAAAGT+TGG | + | chr2_4:12802348-12802367 | Msa0316020:CDS | 25.0% |
| ! | ACAAAATTGAATTCATGTGT+AGG | - | chr2_4:12801739-12801758 | None:intergenic | 25.0% |
| !! | AATTTTGTGATTGATATTGG+AGG | + | chr2_4:12801751-12801770 | Msa0316020:CDS | 25.0% |
| ! | GAAAAAATAGCATCAACTAA+TGG | - | chr2_4:12801940-12801959 | None:intergenic | 25.0% |
| !!! | TTAGTTGATGCTATTTTTTC+AGG | + | chr2_4:12801940-12801959 | Msa0316020:CDS | 25.0% |
| !!! | GAGACTTTGATTTTAGATTT+TGG | - | chr2_4:12801994-12802013 | None:intergenic | 25.0% |
| ! | GATGTGAAATCAATTAAAGT+TGG | + | chr2_4:12802348-12802367 | Msa0316020:CDS | 25.0% |
| ! | AAGTGCTATTGTGAGAATTA+GGG | - | chr2_4:12801620-12801639 | None:intergenic | 30.0% |
| AAGTAAAGTGGAGAATAGAT+TGG | - | chr2_4:12801701-12801720 | None:intergenic | 30.0% | |
| ATCTATTCTCCACTTTACTT+TGG | + | chr2_4:12801701-12801720 | Msa0316020:CDS | 30.0% | |
| TCTATTCTCCACTTTACTTT+GGG | + | chr2_4:12801702-12801721 | Msa0316020:CDS | 30.0% | |
| ! | ATTTTGGATTCACAAGAGAT+GGG | - | chr2_4:12801829-12801848 | None:intergenic | 30.0% |
| ! | AATTTTGGATTCACAAGAGA+TGG | - | chr2_4:12801830-12801849 | None:intergenic | 30.0% |
| ! | AGCATCATTAGTGCAAATTT+TGG | - | chr2_4:12801845-12801864 | None:intergenic | 30.0% |
| AAAATTTGCACTAATGATGC+TGG | + | chr2_4:12801844-12801863 | Msa0316020:CDS | 30.0% | |
| !!! | AGTTTTTGGCAAGTTTTTGA+GGG | - | chr2_4:12802079-12802098 | None:intergenic | 30.0% |
| !!! | TAGTTTTTGGCAAGTTTTTG+AGG | - | chr2_4:12802080-12802099 | None:intergenic | 30.0% |
| AACTTGCCAAAAACTAGTAA+AGG | + | chr2_4:12802084-12802103 | Msa0316020:CDS | 30.0% | |
| !! | TTGGTAGTCTTTTCATTGTT+GGG | + | chr2_4:12802214-12802233 | Msa0316020:CDS | 30.0% |
| CTAAAGGGGTTAATAATAAG+AGG | - | chr2_4:12802279-12802298 | None:intergenic | 30.0% | |
| GTGAAATCAATTAAAGTTGG+TGG | + | chr2_4:12802351-12802370 | Msa0316020:CDS | 30.0% | |
| CTTATTGTCAATGGACAAAA+GGG | - | chr2_4:12802400-12802419 | None:intergenic | 30.0% | |
| ! | CTTTTGTCCATTGACAATAA+GGG | + | chr2_4:12802399-12802418 | Msa0316020:CDS | 30.0% |
| ! | AAGTGCTATTGTGAGAATTA+GGG | - | chr2_4:12801620-12801639 | None:intergenic | 30.0% |
| AAGTAAAGTGGAGAATAGAT+TGG | - | chr2_4:12801701-12801720 | None:intergenic | 30.0% | |
| ATCTATTCTCCACTTTACTT+TGG | + | chr2_4:12801701-12801720 | Msa0316020:CDS | 30.0% | |
| TCTATTCTCCACTTTACTTT+GGG | + | chr2_4:12801702-12801721 | Msa0316020:CDS | 30.0% | |
| ! | ATTTTGGATTCACAAGAGAT+GGG | - | chr2_4:12801829-12801848 | None:intergenic | 30.0% |
| ! | AATTTTGGATTCACAAGAGA+TGG | - | chr2_4:12801830-12801849 | None:intergenic | 30.0% |
| ! | AGCATCATTAGTGCAAATTT+TGG | - | chr2_4:12801845-12801864 | None:intergenic | 30.0% |
| AAAATTTGCACTAATGATGC+TGG | + | chr2_4:12801844-12801863 | Msa0316020:CDS | 30.0% | |
| !!! | AGTTTTTGGCAAGTTTTTGA+GGG | - | chr2_4:12802079-12802098 | None:intergenic | 30.0% |
| !!! | TAGTTTTTGGCAAGTTTTTG+AGG | - | chr2_4:12802080-12802099 | None:intergenic | 30.0% |
| AACTTGCCAAAAACTAGTAA+AGG | + | chr2_4:12802084-12802103 | Msa0316020:CDS | 30.0% | |
| !! | TTGGTAGTCTTTTCATTGTT+GGG | + | chr2_4:12802214-12802233 | Msa0316020:CDS | 30.0% |
| CTAAAGGGGTTAATAATAAG+AGG | - | chr2_4:12802279-12802298 | None:intergenic | 30.0% | |
| GTGAAATCAATTAAAGTTGG+TGG | + | chr2_4:12802351-12802370 | Msa0316020:CDS | 30.0% | |
| CTTATTGTCAATGGACAAAA+GGG | - | chr2_4:12802400-12802419 | None:intergenic | 30.0% | |
| ! | CTTTTGTCCATTGACAATAA+GGG | + | chr2_4:12802399-12802418 | Msa0316020:CDS | 30.0% |
| ! | GAAGTGCTATTGTGAGAATT+AGG | - | chr2_4:12801621-12801640 | None:intergenic | 35.0% |
| ! | TTTTGTTGCAGTCATACCAT+AGG | - | chr2_4:12801783-12801802 | None:intergenic | 35.0% |
| ! | ATTGTAGGTTGAAGAGTTGT+AGG | - | chr2_4:12801806-12801825 | None:intergenic | 35.0% |
| AAAGTCTCTGACTTTATCTC+TGG | + | chr2_4:12802006-12802025 | Msa0316020:CDS | 35.0% | |
| !! | TTTTTGGCAAGTTTTTGAGG+GGG | - | chr2_4:12802077-12802096 | None:intergenic | 35.0% |
| !!! | GTTTTTGGCAAGTTTTTGAG+GGG | - | chr2_4:12802078-12802097 | None:intergenic | 35.0% |
| !!! | AGCTGTTTTGGTAATGAAAG+TGG | - | chr2_4:12802132-12802151 | None:intergenic | 35.0% |
| TTACCATCTTCAAACCAACT+TGG | + | chr2_4:12802195-12802214 | Msa0316020:CDS | 35.0% | |
| CAATGAAAAGACTACCAAGT+TGG | - | chr2_4:12802212-12802231 | None:intergenic | 35.0% | |
| !! | CTTGGTAGTCTTTTCATTGT+TGG | + | chr2_4:12802213-12802232 | Msa0316020:CDS | 35.0% |
| ! | TGGTAGTCTTTTCATTGTTG+GGG | + | chr2_4:12802215-12802234 | Msa0316020:CDS | 35.0% |
| ! | AATAGTAGTGAGTTGAAACC+TGG | - | chr2_4:12802256-12802275 | None:intergenic | 35.0% |
| AATATTGGAGCAGTGCTAAA+GGG | - | chr2_4:12802294-12802313 | None:intergenic | 35.0% | |
| AAATATTGGAGCAGTGCTAA+AGG | - | chr2_4:12802295-12802314 | None:intergenic | 35.0% | |
| !! | TTCATAAGAAGCATCACCTT+CGG | - | chr2_4:12802319-12802338 | None:intergenic | 35.0% |
| CCTTATTGTCAATGGACAAA+AGG | - | chr2_4:12802401-12802420 | None:intergenic | 35.0% | |
| ! | CCTTTTGTCCATTGACAATA+AGG | + | chr2_4:12802398-12802417 | Msa0316020:CDS | 35.0% |
| TCCATTGACAATAAGGGAAA+TGG | + | chr2_4:12802405-12802424 | Msa0316020:CDS | 35.0% | |
| ACCATTTCCCTTATTGTCAA+TGG | - | chr2_4:12802409-12802428 | None:intergenic | 35.0% | |
| ! | GAAGTGCTATTGTGAGAATT+AGG | - | chr2_4:12801621-12801640 | None:intergenic | 35.0% |
| ! | TTTTGTTGCAGTCATACCAT+AGG | - | chr2_4:12801783-12801802 | None:intergenic | 35.0% |
| ! | ATTGTAGGTTGAAGAGTTGT+AGG | - | chr2_4:12801806-12801825 | None:intergenic | 35.0% |
| AAAGTCTCTGACTTTATCTC+TGG | + | chr2_4:12802006-12802025 | Msa0316020:CDS | 35.0% | |
| !! | TTTTTGGCAAGTTTTTGAGG+GGG | - | chr2_4:12802077-12802096 | None:intergenic | 35.0% |
| !!! | GTTTTTGGCAAGTTTTTGAG+GGG | - | chr2_4:12802078-12802097 | None:intergenic | 35.0% |
| !!! | AGCTGTTTTGGTAATGAAAG+TGG | - | chr2_4:12802132-12802151 | None:intergenic | 35.0% |
| TTACCATCTTCAAACCAACT+TGG | + | chr2_4:12802195-12802214 | Msa0316020:CDS | 35.0% | |
| CAATGAAAAGACTACCAAGT+TGG | - | chr2_4:12802212-12802231 | None:intergenic | 35.0% | |
| !! | CTTGGTAGTCTTTTCATTGT+TGG | + | chr2_4:12802213-12802232 | Msa0316020:CDS | 35.0% |
| ! | TGGTAGTCTTTTCATTGTTG+GGG | + | chr2_4:12802215-12802234 | Msa0316020:CDS | 35.0% |
| ! | AATAGTAGTGAGTTGAAACC+TGG | - | chr2_4:12802256-12802275 | None:intergenic | 35.0% |
| AATATTGGAGCAGTGCTAAA+GGG | - | chr2_4:12802294-12802313 | None:intergenic | 35.0% | |
| AAATATTGGAGCAGTGCTAA+AGG | - | chr2_4:12802295-12802314 | None:intergenic | 35.0% | |
| !! | TTCATAAGAAGCATCACCTT+CGG | - | chr2_4:12802319-12802338 | None:intergenic | 35.0% |
| CCTTATTGTCAATGGACAAA+AGG | - | chr2_4:12802401-12802420 | None:intergenic | 35.0% | |
| ! | CCTTTTGTCCATTGACAATA+AGG | + | chr2_4:12802398-12802417 | Msa0316020:CDS | 35.0% |
| TCCATTGACAATAAGGGAAA+TGG | + | chr2_4:12802405-12802424 | Msa0316020:CDS | 35.0% | |
| ACCATTTCCCTTATTGTCAA+TGG | - | chr2_4:12802409-12802428 | None:intergenic | 35.0% | |
| TGCTATTGTGAGAATTAGGG+AGG | - | chr2_4:12801617-12801636 | None:intergenic | 40.0% | |
| ! | TGGAGAATAGATTGGTTGCT+GGG | - | chr2_4:12801693-12801712 | None:intergenic | 40.0% |
| TTCTCCACTTTACTTTGGGT+AGG | + | chr2_4:12801706-12801725 | Msa0316020:CDS | 40.0% | |
| TGTTCCTACCCAAAGTAAAG+TGG | - | chr2_4:12801713-12801732 | None:intergenic | 40.0% | |
| ATATTGGAGGACCAATCCTA+TGG | + | chr2_4:12801764-12801783 | Msa0316020:CDS | 40.0% | |
| TTGCAGTCATACCATAGGAT+TGG | - | chr2_4:12801778-12801797 | None:intergenic | 40.0% | |
| TTCACAAGAGATGGGATTGT+AGG | - | chr2_4:12801821-12801840 | None:intergenic | 40.0% | |
| ! | TTGAGCAACCTGGTTTGAAA+GGG | - | chr2_4:12801888-12801907 | None:intergenic | 40.0% |
| ! | TTTGAGCAACCTGGTTTGAA+AGG | - | chr2_4:12801889-12801908 | None:intergenic | 40.0% |
| ! | CAAGTATTGTTTGAGCAACC+TGG | - | chr2_4:12801898-12801917 | None:intergenic | 40.0% |
| GGTTGCTCAAACAATACTTG+TGG | + | chr2_4:12801898-12801917 | Msa0316020:CDS | 40.0% | |
| !!! | GCTATTTTTTCAGGTGACAC+TGG | + | chr2_4:12801949-12801968 | Msa0316020:CDS | 40.0% |
| ATGAAAGTGGTGTTCTTGCA+AGG | - | chr2_4:12802119-12802138 | None:intergenic | 40.0% | |
| ! | GGAGCTAATGAAAGCTGTTT+TGG | - | chr2_4:12802144-12802163 | None:intergenic | 40.0% |
| !! | CTACCAAGTTGGTTTGAAGA+TGG | - | chr2_4:12802201-12802220 | None:intergenic | 40.0% |
| ! | AGTCTTTTCATTGTTGGGGT+AGG | + | chr2_4:12802219-12802238 | Msa0316020:CDS | 40.0% |
| ATATTGGAGCAGTGCTAAAG+GGG | - | chr2_4:12802293-12802312 | None:intergenic | 40.0% | |
| ACTGCTCCAATATTTACCGA+AGG | + | chr2_4:12802300-12802319 | Msa0316020:CDS | 40.0% | |
| GCATCACCTTCGGTAAATAT+TGG | - | chr2_4:12802309-12802328 | None:intergenic | 40.0% | |
| TGCTATTGTGAGAATTAGGG+AGG | - | chr2_4:12801617-12801636 | None:intergenic | 40.0% | |
| ! | TGGAGAATAGATTGGTTGCT+GGG | - | chr2_4:12801693-12801712 | None:intergenic | 40.0% |
| TTCTCCACTTTACTTTGGGT+AGG | + | chr2_4:12801706-12801725 | Msa0316020:CDS | 40.0% | |
| TGTTCCTACCCAAAGTAAAG+TGG | - | chr2_4:12801713-12801732 | None:intergenic | 40.0% | |
| ATATTGGAGGACCAATCCTA+TGG | + | chr2_4:12801764-12801783 | Msa0316020:CDS | 40.0% | |
| TTGCAGTCATACCATAGGAT+TGG | - | chr2_4:12801778-12801797 | None:intergenic | 40.0% | |
| TTCACAAGAGATGGGATTGT+AGG | - | chr2_4:12801821-12801840 | None:intergenic | 40.0% | |
| ! | TTGAGCAACCTGGTTTGAAA+GGG | - | chr2_4:12801888-12801907 | None:intergenic | 40.0% |
| ! | TTTGAGCAACCTGGTTTGAA+AGG | - | chr2_4:12801889-12801908 | None:intergenic | 40.0% |
| ! | CAAGTATTGTTTGAGCAACC+TGG | - | chr2_4:12801898-12801917 | None:intergenic | 40.0% |
| GGTTGCTCAAACAATACTTG+TGG | + | chr2_4:12801898-12801917 | Msa0316020:CDS | 40.0% | |
| !!! | GCTATTTTTTCAGGTGACAC+TGG | + | chr2_4:12801949-12801968 | Msa0316020:CDS | 40.0% |
| ATGAAAGTGGTGTTCTTGCA+AGG | - | chr2_4:12802119-12802138 | None:intergenic | 40.0% | |
| ! | GGAGCTAATGAAAGCTGTTT+TGG | - | chr2_4:12802144-12802163 | None:intergenic | 40.0% |
| !! | CTACCAAGTTGGTTTGAAGA+TGG | - | chr2_4:12802201-12802220 | None:intergenic | 40.0% |
| ! | AGTCTTTTCATTGTTGGGGT+AGG | + | chr2_4:12802219-12802238 | Msa0316020:CDS | 40.0% |
| ATATTGGAGCAGTGCTAAAG+GGG | - | chr2_4:12802293-12802312 | None:intergenic | 40.0% | |
| ACTGCTCCAATATTTACCGA+AGG | + | chr2_4:12802300-12802319 | Msa0316020:CDS | 40.0% | |
| GCATCACCTTCGGTAAATAT+TGG | - | chr2_4:12802309-12802328 | None:intergenic | 40.0% | |
| !!! | GTTGCTGGGTCTTTTTCAAC+TGG | - | chr2_4:12801679-12801698 | None:intergenic | 45.0% |
| ! | GTGGAGAATAGATTGGTTGC+TGG | - | chr2_4:12801694-12801713 | None:intergenic | 45.0% |
| !!! | GTTGCTGGGTCTTTTTCAAC+TGG | - | chr2_4:12801679-12801698 | None:intergenic | 45.0% |
| ! | GTGGAGAATAGATTGGTTGC+TGG | - | chr2_4:12801694-12801713 | None:intergenic | 45.0% |
| TGCAATGGCCCTTTCAAACC+AGG | + | chr2_4:12801877-12801896 | Msa0316020:CDS | 50.0% | |
| GGGTAGGATCTTCTCTTTCC+AGG | + | chr2_4:12802235-12802254 | Msa0316020:CDS | 50.0% | |
| TGCAATGGCCCTTTCAAACC+AGG | + | chr2_4:12801877-12801896 | Msa0316020:CDS | 50.0% | |
| GGGTAGGATCTTCTCTTTCC+AGG | + | chr2_4:12802235-12802254 | Msa0316020:CDS | 50.0% | |
| GCTGGATGCACTAGCTGCAA+TGG | + | chr2_4:12801862-12801881 | Msa0316020:CDS | 55.0% | |
| GCTGGATGCACTAGCTGCAA+TGG | + | chr2_4:12801862-12801881 | Msa0316020:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 12801589 | 12802431 | 12801589 | ID=Msa0316020;Name=Msa0316020 |
| chr2_4 | mRNA | 12801589 | 12802431 | 12801589 | ID=Msa0316020-mRNA-1;Parent=Msa0316020;Name=Msa0316020-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|280 |
| chr2_4 | exon | 12801589 | 12802431 | 12801589 | ID=Msa0316020-mRNA-1:exon:4873;Parent=Msa0316020-mRNA-1 |
| chr2_4 | CDS | 12801589 | 12802431 | 12801589 | ID=Msa0316020-mRNA-1:cds;Parent=Msa0316020-mRNA-1 |
| Gene Sequence |
| Protein sequence |
