Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1074600 | XP_013449250.1 | 88.785 | 214 | 19 | 3 | 1 | 210 | 1 | 213 | 1.17e-114 | 335 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1074600 | A0A072U0R7 | 88.785 | 214 | 19 | 3 | 1 | 210 | 1 | 213 | 5.58e-115 | 335 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003080 | Msa1074600 | 0.818964 | 1.488723e-52 | -8.615850e-47 |
| Msa0025160 | Msa1074600 | 0.867223 | 1.603352e-65 | -8.615850e-47 |
| Msa0025580 | Msa1074600 | 0.805576 | 1.248734e-49 | -8.615850e-47 |
| Msa0027280 | Msa1074600 | 0.805515 | 1.286392e-49 | -8.615850e-47 |
| Msa0032980 | Msa1074600 | 0.804446 | 2.151689e-49 | -8.615850e-47 |
| Msa0034210 | Msa1074600 | 0.821580 | 3.744003e-53 | -8.615850e-47 |
| Msa0042640 | Msa1074600 | 0.803689 | 3.091584e-49 | -8.615850e-47 |
| Msa0050660 | Msa1074600 | 0.846541 | 2.035540e-59 | -8.615850e-47 |
| Msa0050700 | Msa1074600 | 0.846898 | 1.625828e-59 | -8.615850e-47 |
| Msa0050790 | Msa1074600 | 0.851862 | 6.728575e-61 | -8.615850e-47 |
| Msa0054690 | Msa1074600 | 0.802518 | 5.399830e-49 | -8.615850e-47 |
| Msa0062340 | Msa1074600 | 0.806813 | 6.856239e-50 | -8.615850e-47 |
| Msa0072520 | Msa1074600 | 0.863200 | 2.949308e-64 | -8.615850e-47 |
| Msa0073120 | Msa1074600 | 0.808669 | 2.764179e-50 | -8.615850e-47 |
| Msa0077560 | Msa1074600 | 0.820377 | 7.082799e-53 | -8.615850e-47 |
| Msa0091350 | Msa1074600 | 0.802710 | 4.930679e-49 | -8.615850e-47 |
| Msa0093860 | Msa1074600 | 0.811476 | 6.868079e-51 | -8.615850e-47 |
| Msa0113340 | Msa1074600 | 0.814069 | 1.858729e-51 | -8.615850e-47 |
| Msa0607800 | Msa1074600 | 0.846065 | 2.744748e-59 | -8.615850e-47 |
| Msa0616210 | Msa1074600 | 0.811085 | 8.351391e-51 | -8.615850e-47 |
| Msa0619710 | Msa1074600 | 0.804126 | 2.508467e-49 | -8.615850e-47 |
| Msa0621710 | Msa1074600 | 0.828980 | 6.648901e-55 | -8.615850e-47 |
| Msa0644410 | Msa1074600 | 0.823422 | 1.397322e-53 | -8.615850e-47 |
| Msa0648040 | Msa1074600 | 0.832108 | 1.140900e-55 | -8.615850e-47 |
| Msa0655700 | Msa1074600 | 0.829105 | 6.198796e-55 | -8.615850e-47 |
| Msa0661150 | Msa1074600 | 0.800695 | 1.277664e-48 | -8.615850e-47 |
| Msa0675330 | Msa1074600 | 0.810397 | 1.176152e-50 | -8.615850e-47 |
| Msa0687090 | Msa1074600 | 0.835837 | 1.329762e-56 | -8.615850e-47 |
| Msa0690740 | Msa1074600 | 0.826235 | 3.032282e-54 | -8.615850e-47 |
| Msa0699540 | Msa1074600 | 0.811460 | 6.923319e-51 | -8.615850e-47 |
| Msa0706150 | Msa1074600 | 0.800259 | 1.567677e-48 | -8.615850e-47 |
| Msa0709440 | Msa1074600 | 0.827531 | 1.485921e-54 | -8.615850e-47 |
| Msa0714520 | Msa1074600 | 0.834417 | 3.033105e-56 | -8.615850e-47 |
| Msa0735780 | Msa1074600 | 0.814077 | 1.851566e-51 | -8.615850e-47 |
| Msa0738700 | Msa1074600 | 0.819883 | 9.191112e-53 | -8.615850e-47 |
| Msa0743440 | Msa1074600 | 0.830571 | 2.724548e-55 | -8.615850e-47 |
| Msa0761670 | Msa1074600 | 0.819394 | 1.188406e-52 | -8.615850e-47 |
| Msa0780910 | Msa1074600 | 0.808265 | 3.372326e-50 | -8.615850e-47 |
| Msa0821160 | Msa1074600 | 0.812546 | 4.015629e-51 | -8.615850e-47 |
| Msa0824450 | Msa1074600 | 0.819922 | 9.005708e-53 | -8.615850e-47 |
| Msa0120790 | Msa1074600 | 0.805420 | 1.346668e-49 | -8.615850e-47 |
| Msa0122330 | Msa1074600 | 0.806729 | 7.139529e-50 | -8.615850e-47 |
| Msa0123070 | Msa1074600 | 0.829896 | 3.983219e-55 | -8.615850e-47 |
| Msa0128310 | Msa1074600 | 0.817342 | 3.466391e-52 | -8.615850e-47 |
| Msa0140280 | Msa1074600 | 0.804659 | 1.942263e-49 | -8.615850e-47 |
| Msa0143670 | Msa1074600 | 0.802809 | 4.703657e-49 | -8.615850e-47 |
| Msa0146650 | Msa1074600 | 0.835518 | 1.601815e-56 | -8.615850e-47 |
| Msa0161930 | Msa1074600 | 0.804849 | 1.772994e-49 | -8.615850e-47 |
| Msa0165830 | Msa1074600 | 0.814668 | 1.370575e-51 | -8.615850e-47 |
| Msa0174440 | Msa1074600 | 0.820851 | 5.511828e-53 | -8.615850e-47 |
| Msa0176060 | Msa1074600 | 0.817413 | 3.341413e-52 | -8.615850e-47 |
| Msa0190570 | Msa1074600 | 0.811163 | 8.032035e-51 | -8.615850e-47 |
| Msa0190730 | Msa1074600 | 0.821733 | 3.450915e-53 | -8.615850e-47 |
| Msa0200050 | Msa1074600 | 0.810712 | 1.005707e-50 | -8.615850e-47 |
| Msa0230800 | Msa1074600 | 0.822502 | 2.288876e-53 | -8.615850e-47 |
| Msa0230870 | Msa1074600 | 0.820276 | 7.473124e-53 | -8.615850e-47 |
| Msa0233020 | Msa1074600 | 0.842085 | 3.206995e-58 | -8.615850e-47 |
| Msa0367520 | Msa1074600 | 0.819561 | 1.089018e-52 | -8.615850e-47 |
| Msa0380670 | Msa1074600 | 0.820750 | 5.815221e-53 | -8.615850e-47 |
| Msa0421130 | Msa1074600 | 0.805775 | 1.134229e-49 | -8.615850e-47 |
| Msa0422050 | Msa1074600 | 0.807342 | 5.298316e-50 | -8.615850e-47 |
| Msa0426530 | Msa1074600 | 0.828605 | 8.192582e-55 | -8.615850e-47 |
| Msa0430300 | Msa1074600 | 0.816422 | 5.575847e-52 | -8.615850e-47 |
| Msa0431200 | Msa1074600 | 0.823926 | 1.064707e-53 | -8.615850e-47 |
| Msa0432010 | Msa1074600 | 0.851012 | 1.170918e-60 | -8.615850e-47 |
| Msa0466520 | Msa1074600 | 0.810133 | 1.341333e-50 | -8.615850e-47 |
| Msa0466880 | Msa1074600 | 0.830671 | 2.576087e-55 | -8.615850e-47 |
| Msa0468700 | Msa1074600 | 0.812680 | 3.753462e-51 | -8.615850e-47 |
| Msa0472950 | Msa1074600 | 0.805339 | 1.400198e-49 | -8.615850e-47 |
| Msa0481890 | Msa1074600 | 0.827139 | 1.844942e-54 | -8.615850e-47 |
| Msa0989500 | Msa1074600 | 0.815041 | 1.132440e-51 | -8.615850e-47 |
| Msa1035570 | Msa1074600 | 0.811543 | 6.641511e-51 | -8.615850e-47 |
| Msa1035610 | Msa1074600 | 0.821640 | 3.626190e-53 | -8.615850e-47 |
| Msa1042160 | Msa1074600 | 0.838919 | 2.160495e-57 | -8.615850e-47 |
| Msa1042190 | Msa1074600 | 0.838132 | 3.448373e-57 | -8.615850e-47 |
| Msa1059020 | Msa1074600 | 0.840587 | 7.948619e-58 | -8.615850e-47 |
| Msa1074600 | Msa1080000 | 0.805692 | 1.180596e-49 | -8.615850e-47 |
| Msa1074600 | Msa1081390 | 0.812904 | 3.352843e-51 | -8.615850e-47 |
| Msa1074600 | Msa1084530 | 0.822403 | 2.413095e-53 | -8.615850e-47 |
| Msa1074600 | Msa1116220 | 0.818226 | 2.189096e-52 | -8.615850e-47 |
| Msa1074600 | Msa1116660 | 0.801509 | 8.708914e-49 | -8.615850e-47 |
| Msa1074600 | Msa1123210 | 0.804037 | 2.618582e-49 | -8.615850e-47 |
| Msa1074600 | Msa1125820 | 0.830902 | 2.261048e-55 | -8.615850e-47 |
| Msa1074600 | Msa1125990 | 0.830173 | 3.407971e-55 | -8.615850e-47 |
| Msa1074600 | Msa1132530 | 0.826615 | 2.461074e-54 | -8.615850e-47 |
| Msa1074600 | Msa1166190 | 0.817724 | 2.842499e-52 | -8.615850e-47 |
| Msa1074600 | Msa1166290 | 0.816326 | 5.858753e-52 | -8.615850e-47 |
| Msa1074600 | Msa1167470 | 0.841233 | 5.381069e-58 | -8.615850e-47 |
| Msa1074600 | Msa1171290 | 0.823581 | 1.282173e-53 | -8.615850e-47 |
| Msa1074600 | Msa1194240 | 0.818480 | 1.917547e-52 | -8.615850e-47 |
| Msa1074600 | Msa1211640 | 0.803204 | 3.897243e-49 | -8.615850e-47 |
| Msa1074600 | Msa1215870 | 0.831416 | 1.689801e-55 | -8.615850e-47 |
| Msa1074600 | Msa1217230 | 0.830855 | 2.321982e-55 | -8.615850e-47 |
| Msa1074600 | Msa1223390 | 0.838978 | 2.085484e-57 | -8.615850e-47 |
| Msa1074600 | Msa1254540 | 0.837532 | 4.916638e-57 | -8.615850e-47 |
| Msa1074600 | Msa1262590 | 0.811133 | 8.152418e-51 | -8.615850e-47 |
| Msa1074600 | Msa1263740 | 0.819596 | 1.069153e-52 | -8.615850e-47 |
| Msa1074600 | Msa1263780 | 0.835405 | 1.710539e-56 | -8.615850e-47 |
| Msa1074600 | Msa1294460 | 0.811820 | 5.781306e-51 | -8.615850e-47 |
| Msa1074600 | Msa1306570 | 0.810298 | 1.235735e-50 | -8.615850e-47 |
| Msa1074600 | Msa1327800 | 0.804216 | 2.403098e-49 | -8.615850e-47 |
| Msa1074600 | Msa1332630 | 0.814367 | 1.597446e-51 | -8.615850e-47 |
| Msa1074600 | Msa1332950 | 0.821494 | 3.918175e-53 | -8.615850e-47 |
| Msa1074600 | Msa1342810 | 0.822630 | 2.137406e-53 | -8.615850e-47 |
| Msa1074600 | Msa1343170 | 0.800441 | 1.439189e-48 | -8.615850e-47 |
| Msa1074600 | Msa1343180 | 0.816531 | 5.270141e-52 | -8.615850e-47 |
| Msa1074600 | Msa1359690 | 0.810923 | 9.054712e-51 | -8.615850e-47 |
| Msa1074600 | Msa1364410 | 0.800127 | 1.667225e-48 | -8.615850e-47 |
| Msa1074600 | Msa1364680 | 0.875853 | 2.219726e-68 | -8.615850e-47 |
| Msa1074600 | Msa1368750 | 0.852761 | 3.733887e-61 | -8.615850e-47 |
| Msa1074600 | Msa1374790 | 0.857319 | 1.770510e-62 | -8.615850e-47 |
| Msa1074600 | Msa1375350 | 0.817591 | 3.045414e-52 | -8.615850e-47 |
| Msa1074600 | Msa1383310 | 0.803703 | 3.070915e-49 | -8.615850e-47 |
| Msa1074600 | Msa1393780 | 0.837922 | 3.904907e-57 | -8.615850e-47 |
| Msa1074600 | Msa1405460 | 0.815173 | 1.058661e-51 | -8.615850e-47 |
| Msa1074600 | Msa1406720 | 0.810805 | 9.600512e-51 | -8.615850e-47 |
| Msa1074600 | Msa1409410 | 0.870873 | 1.049660e-66 | -8.615850e-47 |
| Msa1074600 | Msa1410160 | 0.826292 | 2.939686e-54 | -8.615850e-47 |
| Msa1074600 | Msa1411790 | 0.802486 | 5.483876e-49 | -8.615850e-47 |
| Msa1074600 | Msa1415540 | 0.831855 | 1.317364e-55 | -8.615850e-47 |
| Msa1074600 | Msa1431480 | 0.803970 | 2.702867e-49 | -8.615850e-47 |
| Msa1074600 | Msa1437930 | 0.802081 | 6.644183e-49 | -8.615850e-47 |
| Msa1074600 | Msa1440630 | 0.850988 | 1.189254e-60 | -8.615850e-47 |
| Msa1074600 | Msa1442260 | 0.804410 | 2.189665e-49 | -8.615850e-47 |
| Msa1074600 | Msa1450190 | 0.800522 | 1.385274e-48 | -8.615850e-47 |
| Msa1074600 | Msa1451820 | 0.856868 | 2.403778e-62 | -8.615850e-47 |
| Msa1074600 | Msa1452140 | 0.824938 | 6.153056e-54 | -8.615850e-47 |
| Msa0272260 | Msa1074600 | 0.829661 | 4.543285e-55 | -8.615850e-47 |
| Msa0284800 | Msa1074600 | 0.806720 | 7.173342e-50 | -8.615850e-47 |
| Msa0330520 | Msa1074600 | 0.802939 | 4.421739e-49 | -8.615850e-47 |
| Msa0514580 | Msa1074600 | 0.820121 | 8.107620e-53 | -8.615850e-47 |
| Msa0517250 | Msa1074600 | 0.859333 | 4.448516e-63 | -8.615850e-47 |
| Msa0520260 | Msa1074600 | 0.821436 | 4.041881e-53 | -8.615850e-47 |
| Msa0520870 | Msa1074600 | 0.837670 | 4.531302e-57 | -8.615850e-47 |
| Msa0528570 | Msa1074600 | 0.830509 | 2.820946e-55 | -8.615850e-47 |
| Msa0529030 | Msa1074600 | 0.819033 | 1.436513e-52 | -8.615850e-47 |
| Msa0531960 | Msa1074600 | 0.812909 | 3.344520e-51 | -8.615850e-47 |
| Msa0541300 | Msa1074600 | 0.800961 | 1.127033e-48 | -8.615850e-47 |
| Msa0541310 | Msa1074600 | 0.802455 | 5.563933e-49 | -8.615850e-47 |
| Msa0553750 | Msa1074600 | 0.828359 | 9.393290e-55 | -8.615850e-47 |
| Msa0562350 | Msa1074600 | 0.807302 | 5.401194e-50 | -8.615850e-47 |
| Msa0580810 | Msa1074600 | 0.812891 | 3.374487e-51 | -8.615850e-47 |
| Msa0585020 | Msa1074600 | 0.809802 | 1.580467e-50 | -8.615850e-47 |
| Msa0597270 | Msa1074600 | 0.817095 | 3.937856e-52 | -8.615850e-47 |
| Msa0859290 | Msa1074600 | 0.810850 | 9.390330e-51 | -8.615850e-47 |
| Msa0863620 | Msa1074600 | 0.804304 | 2.303492e-49 | -8.615850e-47 |
| Msa0909760 | Msa1074600 | 0.816355 | 5.773002e-52 | -8.615850e-47 |
| Msa0956350 | Msa1074600 | 0.822135 | 2.785066e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1074600 | MtrunA17_Chr7g0245001 | 88.785 | 214 | 19 | 3 | 1 | 210 | 1 | 213 | 1.07e-118 | 335 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1074600 | AT5G01910.1 | 46.715 | 137 | 61 | 2 | 19 | 155 | 4 | 128 | 6.38e-29 | 107 |
| Msa1074600 | AT5G01910.2 | 46.715 | 137 | 61 | 2 | 19 | 155 | 4 | 128 | 6.54e-29 | 106 |
Find 54 sgRNAs with CRISPR-Local
Find 147 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGATCGGTGGGAGTGTTTA+TGG | 0.249767 | 7_2:+72519328 | None:intergenic |
| AGATTAAGAAAGTACCGTTT+TGG | 0.310104 | 7_2:-72518561 | Msa1074600:three_prime_UTR |
| AAATGAGAAGTTGAAAGAAT+TGG | 0.327588 | 7_2:+72519448 | None:intergenic |
| TTATGAAATTCAAATTCAAC+AGG | 0.363852 | 7_2:+72518491 | None:intergenic |
| CGATAGATTGTTCCGTTTGA+AGG | 0.364571 | 7_2:-72519024 | Msa1074600:CDS |
| TTGGAGAATGGTGAATAAAA+AGG | 0.365241 | 7_2:+72519467 | None:intergenic |
| TAATTGATCGAGCTTGGGTT+TGG | 0.365621 | 7_2:+72519283 | None:intergenic |
| AAAACATAATTGATCGAGCT+TGG | 0.395643 | 7_2:+72519277 | None:intergenic |
| TGCATAAGCTGTGCCATGTT+TGG | 0.401680 | 7_2:+72519410 | None:intergenic |
| AATTGATCGAGCTTGGGTTT+GGG | 0.402211 | 7_2:+72519284 | None:intergenic |
| CCGAAAATCCGGCCACGCTC+TGG | 0.408098 | 7_2:-72519368 | Msa1074600:CDS |
| GAAGGTGAAAACAGAGGAAA+TGG | 0.410284 | 7_2:-72518693 | Msa1074600:CDS |
| GAGCTTGGGTTTGGGCTTTG+TGG | 0.415984 | 7_2:+72519292 | None:intergenic |
| CCACCGGAACACCGAAAATC+CGG | 0.421575 | 7_2:-72519379 | Msa1074600:CDS |
| AAGCTGTGCCATGTTTGGTT+TGG | 0.436936 | 7_2:+72519415 | None:intergenic |
| GTGTCCGGCCAGAGCGTGGC+CGG | 0.445137 | 7_2:+72519360 | None:intergenic |
| AAACATAATTGATCGAGCTT+GGG | 0.445986 | 7_2:+72519278 | None:intergenic |
| GTTTGGGCTTTGTGGAAAGA+TGG | 0.456431 | 7_2:+72519300 | None:intergenic |
| CGCCACCGTGACTTCTCCAC+TGG | 0.478503 | 7_2:-72519216 | Msa1074600:CDS |
| TCTGAAGTGCTTCAGAGTCC+TGG | 0.482019 | 7_2:-72518632 | Msa1074600:CDS |
| TATTCGAACCCTTAGAGAAA+AGG | 0.487625 | 7_2:-72518886 | Msa1074600:CDS |
| GTTGCTTGACGCGAAGATGA+AGG | 0.492065 | 7_2:-72519093 | Msa1074600:CDS |
| GGAGTGTTTATGGGCTGAAG+TGG | 0.494925 | 7_2:+72519338 | None:intergenic |
| AGATCGGTGGGAGTGTTTAT+GGG | 0.499443 | 7_2:+72519329 | None:intergenic |
| GAAGTCACGGTGGCGGTGAT+CGG | 0.499844 | 7_2:+72519224 | None:intergenic |
| GATATTTGAGTTCCTTCAAA+CGG | 0.520675 | 7_2:+72519012 | None:intergenic |
| AGATGGTGAGTGAGATCGGT+GGG | 0.522709 | 7_2:+72519317 | None:intergenic |
| AAATCCGGCCACGCTCTGGC+CGG | 0.529930 | 7_2:-72519364 | Msa1074600:CDS |
| GGAAAGATGGTGAGTGAGAT+CGG | 0.533728 | 7_2:+72519313 | None:intergenic |
| AACAGAGGAAATGGTTGAGT+CGG | 0.536566 | 7_2:-72518684 | Msa1074600:CDS |
| CAACCACACCAAACCAAACA+TGG | 0.541236 | 7_2:-72519423 | Msa1074600:exon |
| TCCACTGGAGACGTCGCTTG+CGG | 0.547190 | 7_2:-72519201 | Msa1074600:CDS |
| TTATGGGCTGAAGTGGTGTC+CGG | 0.550025 | 7_2:+72519345 | None:intergenic |
| TCCGCAAGCGACGTCTCCAG+TGG | 0.551194 | 7_2:+72519200 | None:intergenic |
| GACCGCGATGAAGAAGAAGC+TGG | 0.558819 | 7_2:-72519171 | Msa1074600:CDS |
| TGACGCGAAGATGAAGGAGA+TGG | 0.559497 | 7_2:-72519087 | Msa1074600:CDS |
| AGTGGTGTCCGGCCAGAGCG+TGG | 0.560975 | 7_2:+72519356 | None:intergenic |
| ACTGGAGACGTCGCTTGCGG+AGG | 0.568299 | 7_2:-72519198 | Msa1074600:CDS |
| GTGCCATGTTTGGTTTGGTG+TGG | 0.568892 | 7_2:+72519420 | None:intergenic |
| GTTTGTGAACAAGCTGAACC+AGG | 0.569366 | 7_2:+72518614 | None:intergenic |
| ATAGAAAAGCTTCCCCAAAA+CGG | 0.576398 | 7_2:+72518547 | None:intergenic |
| CAGTGGAGAAGTCACGGTGG+CGG | 0.593899 | 7_2:+72519217 | None:intergenic |
| TGAAATCACACCGATCCAGA+AGG | 0.600226 | 7_2:-72519255 | Msa1074600:CDS |
| CTTATGCAAACACCCGCCAC+CGG | 0.606622 | 7_2:-72519395 | Msa1074600:CDS |
| TTTCATACCTCTGATGTCGA+TGG | 0.613539 | 7_2:+72518834 | None:intergenic |
| CGTCTCCAGTGGAGAAGTCA+CGG | 0.624834 | 7_2:+72519211 | None:intergenic |
| GGAACTCAAATATCGATGCA+TGG | 0.636808 | 7_2:-72519003 | Msa1074600:intron |
| CTCCAGCTTCTTCTTCATCG+CGG | 0.643638 | 7_2:+72519169 | None:intergenic |
| AAGTTGAAAGAATTGGAGAA+TGG | 0.649318 | 7_2:+72519455 | None:intergenic |
| AACAGCACCATCGACATCAG+AGG | 0.666142 | 7_2:-72518841 | Msa1074600:intron |
| TGTTCAGAAGGTGAAAACAG+AGG | 0.670712 | 7_2:-72518699 | Msa1074600:intron |
| TATGAAATTCAAATTCAACA+GGG | 0.714468 | 7_2:+72518492 | None:intergenic |
| AAGATGGTGAGTGAGATCGG+TGG | 0.730548 | 7_2:+72519316 | None:intergenic |
| CTCCAGTGGAGAAGTCACGG+TGG | 0.790221 | 7_2:+72519214 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATACATAATTTTCTCGTTA+TGG | - | chr7_2:72519396-72519415 | Msa1074600:CDS | 20.0% |
| !! | TATGAAATTCAAATTCAACA+GGG | + | chr7_2:72519431-72519450 | None:intergenic | 20.0% |
| !! | TTATGAAATTCAAATTCAAC+AGG | + | chr7_2:72519432-72519451 | None:intergenic | 20.0% |
| !!! | ATTTTAAGAATGAATGAAAG+TGG | - | chr7_2:72519491-72519510 | Msa1074600:five_prime_UTR | 20.0% |
| !!! | TATACATAATTTTCTCGTTA+TGG | - | chr7_2:72519396-72519415 | Msa1074600:CDS | 20.0% |
| !! | TATGAAATTCAAATTCAACA+GGG | + | chr7_2:72519431-72519450 | None:intergenic | 20.0% |
| !! | TTATGAAATTCAAATTCAAC+AGG | + | chr7_2:72519432-72519451 | None:intergenic | 20.0% |
| !!! | ATTTTAAGAATGAATGAAAG+TGG | - | chr7_2:72519491-72519510 | Msa1074600:five_prime_UTR | 20.0% |
| ! | AAATGAGAAGTTGAAAGAAT+TGG | + | chr7_2:72518475-72518494 | None:intergenic | 25.0% |
| ! | AACAACAACAACAAAAACAA+AGG | + | chr7_2:72519205-72519224 | None:intergenic | 25.0% |
| ! | AAATGAGAAGTTGAAAGAAT+TGG | + | chr7_2:72518475-72518494 | None:intergenic | 25.0% |
| ! | AACAACAACAACAAAAACAA+AGG | + | chr7_2:72519205-72519224 | None:intergenic | 25.0% |
| TTGGAGAATGGTGAATAAAA+AGG | + | chr7_2:72518456-72518475 | None:intergenic | 30.0% | |
| AAGTTGAAAGAATTGGAGAA+TGG | + | chr7_2:72518468-72518487 | None:intergenic | 30.0% | |
| AAACATAATTGATCGAGCTT+GGG | + | chr7_2:72518645-72518664 | None:intergenic | 30.0% | |
| AAAACATAATTGATCGAGCT+TGG | + | chr7_2:72518646-72518665 | None:intergenic | 30.0% | |
| ! | GATATTTGAGTTCCTTCAAA+CGG | + | chr7_2:72518911-72518930 | None:intergenic | 30.0% |
| AGAAACAAAACGAAACTGAA+TGG | + | chr7_2:72518971-72518990 | None:intergenic | 30.0% | |
| ! | TTCATGTTCCTTTTCTCTAA+GGG | + | chr7_2:72519045-72519064 | None:intergenic | 30.0% |
| ! | TTTCATGTTCCTTTTCTCTA+AGG | + | chr7_2:72519046-72519065 | None:intergenic | 30.0% |
| !!! | TTTTGTTGTTGTTGTTCAGA+AGG | - | chr7_2:72519209-72519228 | Msa1074600:CDS | 30.0% |
| !!! | AGATTAAGAAAGTACCGTTT+TGG | - | chr7_2:72519359-72519378 | Msa1074600:CDS | 30.0% |
| !!! | GATTAAGAAAGTACCGTTTT+GGG | - | chr7_2:72519360-72519379 | Msa1074600:CDS | 30.0% |
| !!! | ATTAAGAAAGTACCGTTTTG+GGG | - | chr7_2:72519361-72519380 | Msa1074600:CDS | 30.0% |
| TTGGAGAATGGTGAATAAAA+AGG | + | chr7_2:72518456-72518475 | None:intergenic | 30.0% | |
| AAGTTGAAAGAATTGGAGAA+TGG | + | chr7_2:72518468-72518487 | None:intergenic | 30.0% | |
| AAACATAATTGATCGAGCTT+GGG | + | chr7_2:72518645-72518664 | None:intergenic | 30.0% | |
| AAAACATAATTGATCGAGCT+TGG | + | chr7_2:72518646-72518665 | None:intergenic | 30.0% | |
| ! | GATATTTGAGTTCCTTCAAA+CGG | + | chr7_2:72518911-72518930 | None:intergenic | 30.0% |
| AGAAACAAAACGAAACTGAA+TGG | + | chr7_2:72518971-72518990 | None:intergenic | 30.0% | |
| ! | TTCATGTTCCTTTTCTCTAA+GGG | + | chr7_2:72519045-72519064 | None:intergenic | 30.0% |
| ! | TTTCATGTTCCTTTTCTCTA+AGG | + | chr7_2:72519046-72519065 | None:intergenic | 30.0% |
| !!! | TTTTGTTGTTGTTGTTCAGA+AGG | - | chr7_2:72519209-72519228 | Msa1074600:CDS | 30.0% |
| !!! | AGATTAAGAAAGTACCGTTT+TGG | - | chr7_2:72519359-72519378 | Msa1074600:CDS | 30.0% |
| !!! | GATTAAGAAAGTACCGTTTT+GGG | - | chr7_2:72519360-72519379 | Msa1074600:CDS | 30.0% |
| !!! | ATTAAGAAAGTACCGTTTTG+GGG | - | chr7_2:72519361-72519380 | Msa1074600:CDS | 30.0% |
| GAGAATGGTGAATAAAAAGG+TGG | + | chr7_2:72518453-72518472 | None:intergenic | 35.0% | |
| !! | TTTTCTCTAAGGGTTCGAAT+AGG | + | chr7_2:72519035-72519054 | None:intergenic | 35.0% |
| TATTCGAACCCTTAGAGAAA+AGG | - | chr7_2:72519034-72519053 | Msa1074600:CDS | 35.0% | |
| ATAGAAAAGCTTCCCCAAAA+CGG | + | chr7_2:72519376-72519395 | None:intergenic | 35.0% | |
| AATGAATGAAAGTGGTCCAT+AGG | - | chr7_2:72519499-72519518 | Msa1074600:five_prime_UTR | 35.0% | |
| GAGAATGGTGAATAAAAAGG+TGG | + | chr7_2:72518453-72518472 | None:intergenic | 35.0% | |
| !! | TTTTCTCTAAGGGTTCGAAT+AGG | + | chr7_2:72519035-72519054 | None:intergenic | 35.0% |
| TATTCGAACCCTTAGAGAAA+AGG | - | chr7_2:72519034-72519053 | Msa1074600:CDS | 35.0% | |
| ATAGAAAAGCTTCCCCAAAA+CGG | + | chr7_2:72519376-72519395 | None:intergenic | 35.0% | |
| TGGTGAATAAAAAGGTGGTG+TGG | + | chr7_2:72518448-72518467 | None:intergenic | 40.0% | |
| AATTGATCGAGCTTGGGTTT+GGG | + | chr7_2:72518639-72518658 | None:intergenic | 40.0% | |
| TAATTGATCGAGCTTGGGTT+TGG | + | chr7_2:72518640-72518659 | None:intergenic | 40.0% | |
| CGATAGATTGTTCCGTTTGA+AGG | - | chr7_2:72518896-72518915 | Msa1074600:CDS | 40.0% | |
| GGAACTCAAATATCGATGCA+TGG | - | chr7_2:72518917-72518936 | Msa1074600:intron | 40.0% | |
| TTTCATACCTCTGATGTCGA+TGG | + | chr7_2:72519089-72519108 | None:intergenic | 40.0% | |
| TGTTCAGAAGGTGAAAACAG+AGG | - | chr7_2:72519221-72519240 | Msa1074600:CDS | 40.0% | |
| GAAGGTGAAAACAGAGGAAA+TGG | - | chr7_2:72519227-72519246 | Msa1074600:CDS | 40.0% | |
| AACAGAGGAAATGGTTGAGT+CGG | - | chr7_2:72519236-72519255 | Msa1074600:CDS | 40.0% | |
| TGGTGAATAAAAAGGTGGTG+TGG | + | chr7_2:72518448-72518467 | None:intergenic | 40.0% | |
| AATTGATCGAGCTTGGGTTT+GGG | + | chr7_2:72518639-72518658 | None:intergenic | 40.0% | |
| TAATTGATCGAGCTTGGGTT+TGG | + | chr7_2:72518640-72518659 | None:intergenic | 40.0% | |
| CGATAGATTGTTCCGTTTGA+AGG | - | chr7_2:72518896-72518915 | Msa1074600:CDS | 40.0% | |
| GGAACTCAAATATCGATGCA+TGG | - | chr7_2:72518917-72518936 | Msa1074600:intron | 40.0% | |
| TTTCATACCTCTGATGTCGA+TGG | + | chr7_2:72519089-72519108 | None:intergenic | 40.0% | |
| TGTTCAGAAGGTGAAAACAG+AGG | - | chr7_2:72519221-72519240 | Msa1074600:CDS | 40.0% | |
| GAAGGTGAAAACAGAGGAAA+TGG | - | chr7_2:72519227-72519246 | Msa1074600:CDS | 40.0% | |
| AACAGAGGAAATGGTTGAGT+CGG | - | chr7_2:72519236-72519255 | Msa1074600:CDS | 40.0% | |
| CAACCACACCAAACCAAACA+TGG | - | chr7_2:72518497-72518516 | Msa1074600:three_prime_UTR | 45.0% | |
| AAGCTGTGCCATGTTTGGTT+TGG | + | chr7_2:72518508-72518527 | None:intergenic | 45.0% | |
| TGCATAAGCTGTGCCATGTT+TGG | + | chr7_2:72518513-72518532 | None:intergenic | 45.0% | |
| !! | AGATCGGTGGGAGTGTTTAT+GGG | + | chr7_2:72518594-72518613 | None:intergenic | 45.0% |
| GGAAAGATGGTGAGTGAGAT+CGG | + | chr7_2:72518610-72518629 | None:intergenic | 45.0% | |
| ! | GTTTGGGCTTTGTGGAAAGA+TGG | + | chr7_2:72518623-72518642 | None:intergenic | 45.0% |
| TGAAATCACACCGATCCAGA+AGG | - | chr7_2:72518665-72518684 | Msa1074600:CDS | 45.0% | |
| ! | GGACAGTTTTCCTTCTGGAT+CGG | + | chr7_2:72518678-72518697 | None:intergenic | 45.0% |
| ! | TGATCGGACAGTTTTCCTTC+TGG | + | chr7_2:72518683-72518702 | None:intergenic | 45.0% |
| GTTTGTGAACAAGCTGAACC+AGG | + | chr7_2:72519309-72519328 | None:intergenic | 45.0% | |
| CAACCACACCAAACCAAACA+TGG | - | chr7_2:72518497-72518516 | Msa1074600:three_prime_UTR | 45.0% | |
| AAGCTGTGCCATGTTTGGTT+TGG | + | chr7_2:72518508-72518527 | None:intergenic | 45.0% | |
| TGCATAAGCTGTGCCATGTT+TGG | + | chr7_2:72518513-72518532 | None:intergenic | 45.0% | |
| !! | AGATCGGTGGGAGTGTTTAT+GGG | + | chr7_2:72518594-72518613 | None:intergenic | 45.0% |
| GGAAAGATGGTGAGTGAGAT+CGG | + | chr7_2:72518610-72518629 | None:intergenic | 45.0% | |
| ! | GTTTGGGCTTTGTGGAAAGA+TGG | + | chr7_2:72518623-72518642 | None:intergenic | 45.0% |
| TGAAATCACACCGATCCAGA+AGG | - | chr7_2:72518665-72518684 | Msa1074600:CDS | 45.0% | |
| ! | GGACAGTTTTCCTTCTGGAT+CGG | + | chr7_2:72518678-72518697 | None:intergenic | 45.0% |
| ! | TGATCGGACAGTTTTCCTTC+TGG | + | chr7_2:72518683-72518702 | None:intergenic | 45.0% |
| GTTTGTGAACAAGCTGAACC+AGG | + | chr7_2:72519309-72519328 | None:intergenic | 45.0% | |
| !! | GTGCCATGTTTGGTTTGGTG+TGG | + | chr7_2:72518503-72518522 | None:intergenic | 50.0% |
| ! | TTATGGGCTGAAGTGGTGTC+CGG | + | chr7_2:72518578-72518597 | None:intergenic | 50.0% |
| ! | GGAGTGTTTATGGGCTGAAG+TGG | + | chr7_2:72518585-72518604 | None:intergenic | 50.0% |
| !! | GAGATCGGTGGGAGTGTTTA+TGG | + | chr7_2:72518595-72518614 | None:intergenic | 50.0% |
| AGATGGTGAGTGAGATCGGT+GGG | + | chr7_2:72518606-72518625 | None:intergenic | 50.0% | |
| AAGATGGTGAGTGAGATCGG+TGG | + | chr7_2:72518607-72518626 | None:intergenic | 50.0% | |
| CTCCAGCTTCTTCTTCATCG+CGG | + | chr7_2:72518754-72518773 | None:intergenic | 50.0% | |
| GTTGCTTGACGCGAAGATGA+AGG | - | chr7_2:72518827-72518846 | Msa1074600:intron | 50.0% | |
| TGACGCGAAGATGAAGGAGA+TGG | - | chr7_2:72518833-72518852 | Msa1074600:intron | 50.0% | |
| AACAGCACCATCGACATCAG+AGG | - | chr7_2:72519079-72519098 | Msa1074600:CDS | 50.0% | |
| ! | TCTGAAGTGCTTCAGAGTCC+TGG | - | chr7_2:72519288-72519307 | Msa1074600:CDS | 50.0% |
| !! | GTGCCATGTTTGGTTTGGTG+TGG | + | chr7_2:72518503-72518522 | None:intergenic | 50.0% |
| ! | TTATGGGCTGAAGTGGTGTC+CGG | + | chr7_2:72518578-72518597 | None:intergenic | 50.0% |
| ! | GGAGTGTTTATGGGCTGAAG+TGG | + | chr7_2:72518585-72518604 | None:intergenic | 50.0% |
| !! | GAGATCGGTGGGAGTGTTTA+TGG | + | chr7_2:72518595-72518614 | None:intergenic | 50.0% |
| AGATGGTGAGTGAGATCGGT+GGG | + | chr7_2:72518606-72518625 | None:intergenic | 50.0% | |
| AAGATGGTGAGTGAGATCGG+TGG | + | chr7_2:72518607-72518626 | None:intergenic | 50.0% | |
| CTCCAGCTTCTTCTTCATCG+CGG | + | chr7_2:72518754-72518773 | None:intergenic | 50.0% | |
| GTTGCTTGACGCGAAGATGA+AGG | - | chr7_2:72518827-72518846 | Msa1074600:intron | 50.0% | |
| TGACGCGAAGATGAAGGAGA+TGG | - | chr7_2:72518833-72518852 | Msa1074600:intron | 50.0% | |
| AACAGCACCATCGACATCAG+AGG | - | chr7_2:72519079-72519098 | Msa1074600:CDS | 50.0% | |
| ! | TCTGAAGTGCTTCAGAGTCC+TGG | - | chr7_2:72519288-72519307 | Msa1074600:CDS | 50.0% |
| CTTATGCAAACACCCGCCAC+CGG | - | chr7_2:72518525-72518544 | Msa1074600:three_prime_UTR | 55.0% | |
| !!! | ATTTTCGGTGTTCCGGTGGC+GGG | + | chr7_2:72518540-72518559 | None:intergenic | 55.0% |
| !!! | GATTTTCGGTGTTCCGGTGG+CGG | + | chr7_2:72518541-72518560 | None:intergenic | 55.0% |
| CCACCGGAACACCGAAAATC+CGG | - | chr7_2:72518541-72518560 | Msa1074600:three_prime_UTR | 55.0% | |
| !!! | TGGCCGGATTTTCGGTGTTC+CGG | + | chr7_2:72518547-72518566 | None:intergenic | 55.0% |
| GAGCTTGGGTTTGGGCTTTG+TGG | + | chr7_2:72518631-72518650 | None:intergenic | 55.0% | |
| CGTCTCCAGTGGAGAAGTCA+CGG | + | chr7_2:72518712-72518731 | None:intergenic | 55.0% | |
| GACCGCGATGAAGAAGAAGC+TGG | - | chr7_2:72518749-72518768 | Msa1074600:intron | 55.0% | |
| CTTATGCAAACACCCGCCAC+CGG | - | chr7_2:72518525-72518544 | Msa1074600:three_prime_UTR | 55.0% | |
| !!! | ATTTTCGGTGTTCCGGTGGC+GGG | + | chr7_2:72518540-72518559 | None:intergenic | 55.0% |
| !!! | GATTTTCGGTGTTCCGGTGG+CGG | + | chr7_2:72518541-72518560 | None:intergenic | 55.0% |
| CCACCGGAACACCGAAAATC+CGG | - | chr7_2:72518541-72518560 | Msa1074600:three_prime_UTR | 55.0% | |
| !!! | TGGCCGGATTTTCGGTGTTC+CGG | + | chr7_2:72518547-72518566 | None:intergenic | 55.0% |
| GAGCTTGGGTTTGGGCTTTG+TGG | + | chr7_2:72518631-72518650 | None:intergenic | 55.0% | |
| CGTCTCCAGTGGAGAAGTCA+CGG | + | chr7_2:72518712-72518731 | None:intergenic | 55.0% | |
| GACCGCGATGAAGAAGAAGC+TGG | - | chr7_2:72518749-72518768 | Msa1074600:intron | 55.0% | |
| !!! | CCGGATTTTCGGTGTTCCGG+TGG | + | chr7_2:72518544-72518563 | None:intergenic | 60.0% |
| ! | CCAGAGCGTGGCCGGATTTT+CGG | + | chr7_2:72518555-72518574 | None:intergenic | 60.0% |
| ! | GAAGTCACGGTGGCGGTGAT+CGG | + | chr7_2:72518699-72518718 | None:intergenic | 60.0% |
| CAGTGGAGAAGTCACGGTGG+CGG | + | chr7_2:72518706-72518725 | None:intergenic | 60.0% | |
| CTCCAGTGGAGAAGTCACGG+TGG | + | chr7_2:72518709-72518728 | None:intergenic | 60.0% | |
| TCCACTGGAGACGTCGCTTG+CGG | - | chr7_2:72518719-72518738 | Msa1074600:intron | 60.0% | |
| !!! | CCGGATTTTCGGTGTTCCGG+TGG | + | chr7_2:72518544-72518563 | None:intergenic | 60.0% |
| ! | CCAGAGCGTGGCCGGATTTT+CGG | + | chr7_2:72518555-72518574 | None:intergenic | 60.0% |
| ! | GAAGTCACGGTGGCGGTGAT+CGG | + | chr7_2:72518699-72518718 | None:intergenic | 60.0% |
| CAGTGGAGAAGTCACGGTGG+CGG | + | chr7_2:72518706-72518725 | None:intergenic | 60.0% | |
| CTCCAGTGGAGAAGTCACGG+TGG | + | chr7_2:72518709-72518728 | None:intergenic | 60.0% | |
| TCCACTGGAGACGTCGCTTG+CGG | - | chr7_2:72518719-72518738 | Msa1074600:intron | 60.0% | |
| CCGAAAATCCGGCCACGCTC+TGG | - | chr7_2:72518552-72518571 | Msa1074600:three_prime_UTR | 65.0% | |
| AAATCCGGCCACGCTCTGGC+CGG | - | chr7_2:72518556-72518575 | Msa1074600:three_prime_UTR | 65.0% | |
| CGCCACCGTGACTTCTCCAC+TGG | - | chr7_2:72518704-72518723 | Msa1074600:intron | 65.0% | |
| TCCGCAAGCGACGTCTCCAG+TGG | + | chr7_2:72518723-72518742 | None:intergenic | 65.0% | |
| ACTGGAGACGTCGCTTGCGG+AGG | - | chr7_2:72518722-72518741 | Msa1074600:intron | 65.0% | |
| CCGAAAATCCGGCCACGCTC+TGG | - | chr7_2:72518552-72518571 | Msa1074600:three_prime_UTR | 65.0% | |
| AAATCCGGCCACGCTCTGGC+CGG | - | chr7_2:72518556-72518575 | Msa1074600:three_prime_UTR | 65.0% | |
| CGCCACCGTGACTTCTCCAC+TGG | - | chr7_2:72518704-72518723 | Msa1074600:intron | 65.0% | |
| TCCGCAAGCGACGTCTCCAG+TGG | + | chr7_2:72518723-72518742 | None:intergenic | 65.0% | |
| ACTGGAGACGTCGCTTGCGG+AGG | - | chr7_2:72518722-72518741 | Msa1074600:intron | 65.0% | |
| AGTGGTGTCCGGCCAGAGCG+TGG | + | chr7_2:72518567-72518586 | None:intergenic | 70.0% | |
| AGTGGTGTCCGGCCAGAGCG+TGG | + | chr7_2:72518567-72518586 | None:intergenic | 70.0% | |
| GTGTCCGGCCAGAGCGTGGC+CGG | + | chr7_2:72518563-72518582 | None:intergenic | 75.0% | |
| GTGTCCGGCCAGAGCGTGGC+CGG | + | chr7_2:72518563-72518582 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 72518422 | 72519520 | 72518422 | ID=Msa1074600;Name=Msa1074600 |
| chr7_2 | mRNA | 72518422 | 72519520 | 72518422 | ID=Msa1074600-mRNA-1;Parent=Msa1074600;Name=Msa1074600-mRNA-1;_AED=0.04;_eAED=0.04;_QI=94|1|1|1|1|1|3|161|210 |
| chr7_2 | exon | 72518422 | 72518714 | 72518422 | ID=Msa1074600-mRNA-1:exon:16718;Parent=Msa1074600-mRNA-1 |
| chr7_2 | exon | 72518842 | 72518919 | 72518842 | ID=Msa1074600-mRNA-1:exon:16717;Parent=Msa1074600-mRNA-1 |
| chr7_2 | exon | 72519004 | 72519520 | 72519004 | ID=Msa1074600-mRNA-1:exon:16716;Parent=Msa1074600-mRNA-1 |
| chr7_2 | five_prime_UTR | 72519427 | 72519520 | 72519427 | ID=Msa1074600-mRNA-1:five_prime_utr;Parent=Msa1074600-mRNA-1 |
| chr7_2 | CDS | 72519004 | 72519426 | 72519004 | ID=Msa1074600-mRNA-1:cds;Parent=Msa1074600-mRNA-1 |
| chr7_2 | CDS | 72518842 | 72518919 | 72518842 | ID=Msa1074600-mRNA-1:cds;Parent=Msa1074600-mRNA-1 |
| chr7_2 | CDS | 72518583 | 72518714 | 72518583 | ID=Msa1074600-mRNA-1:cds;Parent=Msa1074600-mRNA-1 |
| chr7_2 | three_prime_UTR | 72518422 | 72518582 | 72518422 | ID=Msa1074600-mRNA-1:three_prime_utr;Parent=Msa1074600-mRNA-1 |
| Gene Sequence |
| Protein sequence |
