AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0314620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0314620 MtrunA17_Chr2g0285001 96.667 180 6 0 1 180 198 377 8.02e-130 369
Msa0314620 MtrunA17_Chr2g0284991 70.109 184 51 1 1 180 197 380 1.63e-86 259
Msa0314620 MtrunA17_Chr2g0284951 72.222 180 45 2 1 180 197 371 1.85e-85 256
Msa0314620 MtrunA17_Chr2g0284941 65.556 180 41 2 1 180 197 355 2.27e-74 227
Msa0314620 MtrunA17_Chr2g0284921 50.617 162 78 2 2 163 201 360 1.00e-49 164
Msa0314620 MtrunA17_Chr3g0085881 47.531 162 79 4 2 163 197 352 6.82e-40 138
Msa0314620 MtrunA17_Chr8g0380041 43.529 170 86 6 2 163 195 362 1.84e-37 132
Msa0314620 MtrunA17_Chr8g0380031 44.512 164 85 4 1 163 247 405 1.56e-36 130
Msa0314620 MtrunA17_Chr2g0284931 51.240 121 57 2 43 163 101 219 1.35e-34 121
Msa0314620 MtrunA17_Chr2g0284981 59.140 93 34 1 92 180 8 100 2.90e-33 114
Msa0314620 MtrunA17_Chr3g0077571 36.686 169 96 5 2 163 192 356 2.22e-30 113
Msa0314620 MtrunA17_Chr3g0086641 37.059 170 97 6 2 163 202 369 6.33e-30 112
Msa0314620 MtrunA17_Chr2g0285061 35.882 170 99 6 2 163 197 364 1.81e-28 108
Msa0314620 MtrunA17_Chr7g0215961 33.728 169 100 4 2 162 186 350 1.59e-24 97.8
Msa0314620 MtrunA17_Chr7g0275011 33.136 169 101 4 2 162 200 364 1.91e-24 97.8
Msa0314620 MtrunA17_Chr3g0131321 35.057 174 100 6 2 166 202 371 2.78e-24 97.4
Msa0314620 MtrunA17_Chr7g0274991 32.738 168 103 4 2 162 199 363 4.53e-23 94.0
Msa0314620 MtrunA17_Chr5g0434621 34.868 152 89 4 2 146 191 339 2.55e-22 92.0
Msa0314620 MtrunA17_Chr6g0457921 37.415 147 80 4 2 139 196 339 1.01e-21 90.5
Msa0314620 MtrunA17_Chr4g0017431 32.558 172 101 7 2 163 196 362 2.47e-21 89.4
Msa0314620 MtrunA17_Chr2g0285031 53.333 75 35 0 93 167 7 81 4.91e-21 82.4
Msa0314620 MtrunA17_Chr3g0077581 30.814 172 105 5 1 162 201 368 6.18e-21 88.2
Msa0314620 MtrunA17_Chr7g0275021 32.143 168 104 4 2 162 200 364 1.04e-20 87.8
Msa0314620 MtrunA17_Chr1g0189891 30.460 174 109 4 2 170 196 362 1.18e-20 87.4
Msa0314620 MtrunA17_Chr5g0444401 30.588 170 104 5 1 162 197 360 1.90e-20 87.0
Msa0314620 MtrunA17_Chr1g0189931 30.409 171 109 4 2 165 198 365 1.93e-20 87.0
Msa0314620 MtrunA17_Chr1g0189901 29.825 171 110 4 2 165 197 364 5.03e-20 85.9
Msa0314620 MtrunA17_Chr1g0182811 31.902 163 101 4 10 165 209 368 8.14e-20 85.1
Msa0314620 MtrunA17_Chr6g0455661 31.953 169 103 4 2 163 308 471 3.72e-19 84.0
Msa0314620 MtrunA17_Chr4g0008851 31.293 147 93 2 2 140 198 344 5.40e-19 82.8
Msa0314620 MtrunA17_Chr6g0459921 31.953 169 103 4 2 163 197 360 6.07e-19 82.8
Msa0314620 MtrunA17_Chr7g0275001 30.178 169 107 5 2 162 191 356 1.05e-18 82.0
Msa0314620 MtrunA17_Chr2g0294311 30.286 175 104 6 1 163 204 372 3.37e-18 80.9
Msa0314620 MtrunA17_Chr5g0396511 28.488 172 113 6 2 165 211 380 5.37e-18 80.1
Msa0314620 MtrunA17_Chr4g0060811 36.842 152 75 6 2 140 194 337 1.26e-17 79.0
Msa0314620 MtrunA17_Chr3g0099231 28.090 178 114 5 1 170 196 367 1.37e-17 79.0
Msa0314620 MtrunA17_Chr3g0092131 32.215 149 88 5 2 141 190 334 2.51e-17 78.2
Msa0314620 MtrunA17_Chr4g0068441 30.460 174 105 6 1 163 199 367 6.33e-17 77.0
Msa0314620 MtrunA17_Chr3g0099151 28.070 171 108 6 1 162 256 420 1.67e-16 76.3
Msa0314620 MtrunA17_Chr5g0405821 35.417 144 76 6 10 140 217 356 7.76e-16 73.9
Msa0314620 MtrunA17_Chr3g0085231 28.743 167 101 6 2 163 196 349 3.08e-15 72.4
Msa0314620 MtrunA17_Chr8g0370961 28.395 162 100 7 10 161 87 242 1.23e-14 69.7
Msa0314620 MtrunA17_Chr2g0294301 29.530 149 93 5 2 140 200 346 1.91e-14 70.1
Msa0314620 MtrunA17_Chr5g0405831 34.783 138 81 5 10 138 216 353 4.34e-14 68.9
Msa0314620 MtrunA17_Chr8g0374101 26.036 169 113 6 2 161 208 373 4.89e-14 68.9
Msa0314620 MtrunA17_Chr5g0420811 29.139 151 95 4 2 144 190 336 4.01e-13 66.2
Msa0314620 MtrunA17_Chr2g0320671 33.333 147 90 5 2 140 194 340 6.56e-13 65.9
Msa0314620 MtrunA17_Chr1g0156931 31.655 139 85 6 10 139 212 349 1.34e-11 62.0
Msa0314620 MtrunA17_Chr7g0226911 27.778 180 91 4 1 147 199 372 2.49e-11 61.2
Msa0314620 MtrunA17_Chr5g0438631 28.873 142 88 5 10 140 200 339 5.30e-11 60.1
Msa0314620 MtrunA17_Chr2g0330781 31.169 154 85 7 2 140 195 342 5.99e-11 60.1
Msa0314620 MtrunA17_Chr3g0120861 30.714 140 87 5 10 140 210 348 7.73e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0314620 AT1G29670.1 43.195 169 87 5 2 163 196 362 2.54e-37 132
Msa0314620 AT1G29670.2 43.195 169 87 5 2 163 264 430 1.59e-36 131
Msa0314620 AT4G18970.1 38.824 170 94 6 2 163 193 360 1.06e-30 114
Msa0314620 AT1G29660.1 40.000 170 92 6 2 163 196 363 2.32e-30 114
Msa0314620 AT4G18970.2 38.824 170 94 6 2 163 193 360 3.51e-30 114
Msa0314620 AT5G45670.1 39.286 168 92 6 2 161 194 359 1.36e-29 112
Msa0314620 AT1G71691.1 34.132 167 99 6 2 161 117 279 3.09e-26 101
Msa0314620 AT1G71691.2 34.132 167 99 6 2 161 218 380 2.83e-25 100
Msa0314620 AT5G15720.3 34.302 172 99 5 2 163 165 332 4.02e-25 99.4
Msa0314620 AT5G15720.1 34.302 172 99 5 2 163 196 363 9.79e-25 99.0
Msa0314620 AT1G33811.1 30.857 175 105 5 1 163 199 369 1.71e-24 98.2
Msa0314620 AT5G08460.1 33.529 170 101 5 2 161 215 382 5.11e-24 97.4
Msa0314620 AT2G19050.1 37.415 147 88 3 1 144 188 333 2.07e-22 92.4
Msa0314620 AT2G19010.1 36.913 149 90 3 1 146 187 334 2.64e-22 92.0
Msa0314620 AT2G19010.2 36.913 149 90 3 1 146 217 364 4.39e-22 92.0
Msa0314620 AT4G30140.1 33.735 166 100 4 1 163 190 348 2.41e-21 89.7
Msa0314620 AT1G71250.1 31.792 173 97 6 2 161 206 370 7.81e-21 88.6
Msa0314620 AT5G33370.1 29.762 168 108 4 2 162 196 360 1.17e-20 87.8
Msa0314620 AT3G04290.1 30.909 165 102 4 2 158 195 355 3.41e-20 86.7
Msa0314620 AT2G19060.1 35.374 147 91 4 2 144 188 334 5.68e-20 85.9
Msa0314620 AT5G18430.1 33.333 156 91 4 2 144 195 350 1.95e-18 81.6
Msa0314620 AT5G37690.2 35.333 150 79 6 2 140 185 327 4.52e-18 80.5
Msa0314620 AT5G37690.1 35.333 150 79 6 2 140 193 335 4.88e-18 80.5
Msa0314620 AT5G41890.1 30.000 180 101 8 1 161 199 372 3.76e-17 78.2
Msa0314620 AT4G16233.1 33.835 133 71 6 45 166 6 132 3.11e-16 71.6
Msa0314620 AT1G74460.1 32.886 149 87 5 2 141 190 334 1.49e-15 73.6
Msa0314620 AT2G04570.1 32.432 148 88 5 10 147 200 345 2.28e-15 73.2
Msa0314620 AT4G28780.1 28.235 170 109 5 2 162 196 361 2.28e-15 73.2
Msa0314620 AT2G23540.1 30.872 149 91 4 2 140 219 365 5.01e-15 72.4
Msa0314620 AT5G03820.1 36.913 149 84 5 2 140 197 345 8.13e-15 71.6
Msa0314620 AT5G03820.2 36.913 149 84 5 2 140 225 373 1.11e-14 71.2
Msa0314620 AT3G50400.1 28.994 169 109 5 2 161 205 371 4.45e-13 66.6
Msa0314620 AT4G26790.1 31.884 138 82 5 13 140 204 339 7.95e-13 65.9
Msa0314620 AT4G26790.2 31.884 138 82 5 13 140 204 339 7.95e-13 65.9
Msa0314620 AT5G45950.1 27.857 140 96 3 11 146 212 350 2.57e-12 64.3
Msa0314620 AT2G40250.1 27.922 154 98 5 1 141 198 351 3.30e-12 63.9
Msa0314620 AT3G16370.1 31.169 154 87 7 2 141 194 342 8.26e-12 62.8
Msa0314620 AT5G42170.1 31.429 140 85 7 10 139 219 357 1.94e-11 62.0
Msa0314620 AT1G23500.1 33.333 144 68 7 10 140 205 333 2.38e-11 61.6
Msa0314620 AT5G55050.1 24.260 169 118 5 2 162 208 374 2.41e-11 61.6
Msa0314620 AT1G71120.1 25.143 175 119 7 1 166 189 360 6.68e-11 60.5
Msa0314620 AT2G03980.2 26.923 156 98 4 2 144 203 355 6.81e-11 60.5
Msa0314620 AT2G03980.3 26.923 156 98 4 2 144 203 355 6.81e-11 60.5
Msa0314620 AT2G03980.1 26.923 156 98 4 2 144 203 355 6.81e-11 60.5
Msa0314620 AT2G03980.4 26.923 156 98 4 2 144 203 355 6.81e-11 60.5

Find 44 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACGATTCATCTAATCATTC+AGG 0.214296 2_4:-10446874 Msa0314620:CDS
CACAATCCAACCGAGCTTAT+TGG 0.237913 2_4:+10446997 None:intergenic
ATTGTTTATTAAGATGAATT+TGG 0.259704 2_4:+10447197 None:intergenic
AGAGTTATCATTGAATTTAC+AGG 0.290001 2_4:-10447519 Msa0314620:intron
GTTACCTGGGTTAGGCCTAT+TGG 0.314894 2_4:-10447355 Msa0314620:CDS
ATAGGAGGGAATATGTGTTT+TGG 0.340020 2_4:-10446948 Msa0314620:CDS
TTACCTGGGTTAGGCCTATT+GGG 0.351337 2_4:-10447354 Msa0314620:CDS
ACAAGAAATCGAGATGGAAT+TGG 0.367083 2_4:-10446815 Msa0314620:CDS
TAACAGGCTTTACATGATCT+TGG 0.383167 2_4:-10447393 Msa0314620:intron
AAACACATATTCCCTCCTAT+TGG 0.405109 2_4:+10446951 None:intergenic
ATCCTTACTCATGAGACATA+TGG 0.416628 2_4:-10447315 Msa0314620:CDS
ATGAAAGGCCATGCTCCAAT+AGG 0.466399 2_4:-10446966 Msa0314620:CDS
ACCCATATGTCTCATGAGTA+AGG 0.468364 2_4:+10447313 None:intergenic
CAGTCCATCCCGGCGAGGCT+TGG 0.480459 2_4:-10446921 Msa0314620:CDS
ACAAGGTGCTTAATATCCAT+TGG 0.485696 2_4:+10446841 None:intergenic
TCCTTACTCATGAGACATAT+GGG 0.490931 2_4:-10447314 Msa0314620:CDS
TCAACTGCCGAGCATTCATA+TGG 0.495949 2_4:-10447165 Msa0314620:intron
AAATATGTGTTACCTGGGTT+AGG 0.497677 2_4:-10447363 Msa0314620:CDS
TGTTGTCCAATAAGCTCGGT+TGG 0.500248 2_4:-10447003 Msa0314620:CDS
CACTTGGAGTGCATCCCAAT+AGG 0.506715 2_4:+10447340 None:intergenic
ACGATTCATCTAATCATTCA+GGG 0.515961 2_4:-10446873 Msa0314620:CDS
TCCTTGTTGTCCAATAAGCT+CGG 0.516114 2_4:-10447007 Msa0314620:CDS
GGTTCCAAGCCTCGCCGGGA+TGG 0.517773 2_4:+10446917 None:intergenic
TTCAGGGTTCGCTTATCCAA+TGG 0.529308 2_4:-10446857 Msa0314620:CDS
GCAAGAAAATATGTGTTACC+TGG 0.530877 2_4:-10447369 Msa0314620:CDS
AGTAGGTTCCAAGCCTCGCC+GGG 0.532908 2_4:+10446913 None:intergenic
ATTATTACCATATGAATGCT+CGG 0.539271 2_4:+10447158 None:intergenic
AAAGGCCATGCTCCAATAGG+AGG 0.561115 2_4:-10446963 Msa0314620:CDS
AGATGAATCGTACGATGTTG+TGG 0.562928 2_4:+10446885 None:intergenic
GAGTAGGTTCCAAGCCTCGC+CGG 0.564674 2_4:+10446912 None:intergenic
CAAGAAAATATGTGTTACCT+GGG 0.577135 2_4:-10447368 Msa0314620:CDS
TCATGAGTAAGGATGGCACT+TGG 0.585430 2_4:+10447324 None:intergenic
ATATGTCTCATGAGTAAGGA+TGG 0.596046 2_4:+10447317 None:intergenic
GATTGTGTATTCGTGATGAA+AGG 0.604626 2_4:-10446981 Msa0314620:CDS
TATTCCCTCCTATTGGAGCA+TGG 0.619481 2_4:+10446958 None:intergenic
ACGATGTTGTGGCAATGAGT+AGG 0.619695 2_4:+10446896 None:intergenic
TGTTGAACAAGAAATCGAGA+TGG 0.622740 2_4:-10446821 Msa0314620:CDS
TCTCGATTTCTTGTTCAACA+AGG 0.622988 2_4:+10446824 None:intergenic
ACTTGAACATATTTAAGCAG+AGG 0.627899 2_4:+10446771 None:intergenic
GACATATGGGTCTTGTGTTG+AGG 0.634040 2_4:-10447301 Msa0314620:CDS
GGATGCAGTCCATCCCGGCG+AGG 0.639170 2_4:-10446926 Msa0314620:CDS
AAGGCCATGCTCCAATAGGA+GGG 0.650431 2_4:-10446962 Msa0314620:CDS
ACCGAGCTTATTGGACAACA+AGG 0.654970 2_4:+10447006 None:intergenic
CATCCCAATAGGCCTAACCC+AGG 0.697491 2_4:+10447351 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGTTTATTAAGATGAATT+TGG + chr2_4:10447119-10447138 None:intergenic 15.0%
!! ATTGTTTATTAAGATGAATT+TGG + chr2_4:10447119-10447138 None:intergenic 15.0%
!! TACAATAGTTATGAATATGA+TGG - chr2_4:10446871-10446890 Msa0314620:CDS 20.0%
!! TTTAAGCTTGAAATCAAAAA+TGG + chr2_4:10447056-10447075 None:intergenic 20.0%
!! TACAATAGTTATGAATATGA+TGG - chr2_4:10446871-10446890 Msa0314620:CDS 20.0%
!! TTTAAGCTTGAAATCAAAAA+TGG + chr2_4:10447056-10447075 None:intergenic 20.0%
! AGAGTTATCATTGAATTTAC+AGG - chr2_4:10446794-10446813 Msa0314620:CDS 25.0%
!!! TGGTTTATAATAGAAGCATT+TGG - chr2_4:10446891-10446910 Msa0314620:CDS 25.0%
! ATTATTACCATATGAATGCT+CGG + chr2_4:10447158-10447177 None:intergenic 25.0%
! AGAGTTATCATTGAATTTAC+AGG - chr2_4:10446794-10446813 Msa0314620:CDS 25.0%
!!! TGGTTTATAATAGAAGCATT+TGG - chr2_4:10446891-10446910 Msa0314620:CDS 25.0%
! ATTATTACCATATGAATGCT+CGG + chr2_4:10447158-10447177 None:intergenic 25.0%
AAGCATTTGGTATATGTAAC+AGG - chr2_4:10446904-10446923 Msa0314620:CDS 30.0%
CAAGAAAATATGTGTTACCT+GGG - chr2_4:10446945-10446964 Msa0314620:CDS 30.0%
! TCAATGAATCTCAGGTTTTT+CGG - chr2_4:10447273-10447292 Msa0314620:CDS 30.0%
TACGATTCATCTAATCATTC+AGG - chr2_4:10447439-10447458 Msa0314620:intron 30.0%
ACGATTCATCTAATCATTCA+GGG - chr2_4:10447440-10447459 Msa0314620:intron 30.0%
AAGCATTTGGTATATGTAAC+AGG - chr2_4:10446904-10446923 Msa0314620:CDS 30.0%
CAAGAAAATATGTGTTACCT+GGG - chr2_4:10446945-10446964 Msa0314620:CDS 30.0%
! TCAATGAATCTCAGGTTTTT+CGG - chr2_4:10447273-10447292 Msa0314620:CDS 30.0%
TACGATTCATCTAATCATTC+AGG - chr2_4:10447439-10447458 Msa0314620:intron 30.0%
ACGATTCATCTAATCATTCA+GGG - chr2_4:10447440-10447459 Msa0314620:intron 30.0%
GTGAAACACAAAGTTCAAGT+GGG + chr2_4:10446822-10446841 None:intergenic 35.0%
AGTGAAACACAAAGTTCAAG+TGG + chr2_4:10446823-10446842 None:intergenic 35.0%
TAACAGGCTTTACATGATCT+TGG - chr2_4:10446920-10446939 Msa0314620:CDS 35.0%
GCAAGAAAATATGTGTTACC+TGG - chr2_4:10446944-10446963 Msa0314620:CDS 35.0%
AAATATGTGTTACCTGGGTT+AGG - chr2_4:10446950-10446969 Msa0314620:CDS 35.0%
ATATGTCTCATGAGTAAGGA+TGG + chr2_4:10446999-10447018 None:intergenic 35.0%
ATCCTTACTCATGAGACATA+TGG - chr2_4:10446998-10447017 Msa0314620:CDS 35.0%
TCCTTACTCATGAGACATAT+GGG - chr2_4:10446999-10447018 Msa0314620:CDS 35.0%
CAGTTTAATGTGATTGATCG+AGG - chr2_4:10447221-10447240 Msa0314620:CDS 35.0%
GATTGTGTATTCGTGATGAA+AGG - chr2_4:10447332-10447351 Msa0314620:CDS 35.0%
AAACACATATTCCCTCCTAT+TGG + chr2_4:10447365-10447384 None:intergenic 35.0%
! ATAGGAGGGAATATGTGTTT+TGG - chr2_4:10447365-10447384 Msa0314620:CDS 35.0%
! TAGGAGGGAATATGTGTTTT+GGG - chr2_4:10447366-10447385 Msa0314620:CDS 35.0%
! ACAAGGTGCTTAATATCCAT+TGG + chr2_4:10447475-10447494 None:intergenic 35.0%
TCTCGATTTCTTGTTCAACA+AGG + chr2_4:10447492-10447511 None:intergenic 35.0%
TGTTGAACAAGAAATCGAGA+TGG - chr2_4:10447492-10447511 Msa0314620:intron 35.0%
ACAAGAAATCGAGATGGAAT+TGG - chr2_4:10447498-10447517 Msa0314620:intron 35.0%
GTGAAACACAAAGTTCAAGT+GGG + chr2_4:10446822-10446841 None:intergenic 35.0%
AGTGAAACACAAAGTTCAAG+TGG + chr2_4:10446823-10446842 None:intergenic 35.0%
TAACAGGCTTTACATGATCT+TGG - chr2_4:10446920-10446939 Msa0314620:CDS 35.0%
GCAAGAAAATATGTGTTACC+TGG - chr2_4:10446944-10446963 Msa0314620:CDS 35.0%
AAATATGTGTTACCTGGGTT+AGG - chr2_4:10446950-10446969 Msa0314620:CDS 35.0%
ATATGTCTCATGAGTAAGGA+TGG + chr2_4:10446999-10447018 None:intergenic 35.0%
ATCCTTACTCATGAGACATA+TGG - chr2_4:10446998-10447017 Msa0314620:CDS 35.0%
TCCTTACTCATGAGACATAT+GGG - chr2_4:10446999-10447018 Msa0314620:CDS 35.0%
CAGTTTAATGTGATTGATCG+AGG - chr2_4:10447221-10447240 Msa0314620:CDS 35.0%
GATTGTGTATTCGTGATGAA+AGG - chr2_4:10447332-10447351 Msa0314620:CDS 35.0%
AAACACATATTCCCTCCTAT+TGG + chr2_4:10447365-10447384 None:intergenic 35.0%
! ATAGGAGGGAATATGTGTTT+TGG - chr2_4:10447365-10447384 Msa0314620:CDS 35.0%
! TAGGAGGGAATATGTGTTTT+GGG - chr2_4:10447366-10447385 Msa0314620:CDS 35.0%
! ACAAGGTGCTTAATATCCAT+TGG + chr2_4:10447475-10447494 None:intergenic 35.0%
TCTCGATTTCTTGTTCAACA+AGG + chr2_4:10447492-10447511 None:intergenic 35.0%
TGTTGAACAAGAAATCGAGA+TGG - chr2_4:10447492-10447511 Msa0314620:intron 35.0%
ACAAGAAATCGAGATGGAAT+TGG - chr2_4:10447498-10447517 Msa0314620:intron 35.0%
ACCCATATGTCTCATGAGTA+AGG + chr2_4:10447003-10447022 None:intergenic 40.0%
CTGCATGTTCAATGAATCTC+AGG - chr2_4:10447265-10447284 Msa0314620:CDS 40.0%
!! CTCAGGTTTTTCGGTTTCAA+TGG - chr2_4:10447282-10447301 Msa0314620:CDS 40.0%
! TCCTTGTTGTCCAATAAGCT+CGG - chr2_4:10447306-10447325 Msa0314620:CDS 40.0%
AGATGAATCGTACGATGTTG+TGG + chr2_4:10447431-10447450 None:intergenic 40.0%
ACCCATATGTCTCATGAGTA+AGG + chr2_4:10447003-10447022 None:intergenic 40.0%
CTGCATGTTCAATGAATCTC+AGG - chr2_4:10447265-10447284 Msa0314620:CDS 40.0%
!! CTCAGGTTTTTCGGTTTCAA+TGG - chr2_4:10447282-10447301 Msa0314620:CDS 40.0%
! TCCTTGTTGTCCAATAAGCT+CGG - chr2_4:10447306-10447325 Msa0314620:CDS 40.0%
AGATGAATCGTACGATGTTG+TGG + chr2_4:10447431-10447450 None:intergenic 40.0%
! TTACCTGGGTTAGGCCTATT+GGG - chr2_4:10446959-10446978 Msa0314620:CDS 45.0%
TCATGAGTAAGGATGGCACT+TGG + chr2_4:10446992-10447011 None:intergenic 45.0%
! GACATATGGGTCTTGTGTTG+AGG - chr2_4:10447012-10447031 Msa0314620:CDS 45.0%
TCAACTGCCGAGCATTCATA+TGG - chr2_4:10447148-10447167 Msa0314620:intron 45.0%
ACCGAGCTTATTGGACAACA+AGG + chr2_4:10447310-10447329 None:intergenic 45.0%
TGTTGTCCAATAAGCTCGGT+TGG - chr2_4:10447310-10447329 Msa0314620:CDS 45.0%
CACAATCCAACCGAGCTTAT+TGG + chr2_4:10447319-10447338 None:intergenic 45.0%
ATGAAAGGCCATGCTCCAAT+AGG - chr2_4:10447347-10447366 Msa0314620:CDS 45.0%
TATTCCCTCCTATTGGAGCA+TGG + chr2_4:10447358-10447377 None:intergenic 45.0%
ACGATGTTGTGGCAATGAGT+AGG + chr2_4:10447420-10447439 None:intergenic 45.0%
TTCAGGGTTCGCTTATCCAA+TGG - chr2_4:10447456-10447475 Msa0314620:intron 45.0%
! TTACCTGGGTTAGGCCTATT+GGG - chr2_4:10446959-10446978 Msa0314620:CDS 45.0%
TCATGAGTAAGGATGGCACT+TGG + chr2_4:10446992-10447011 None:intergenic 45.0%
! GACATATGGGTCTTGTGTTG+AGG - chr2_4:10447012-10447031 Msa0314620:CDS 45.0%
TCAACTGCCGAGCATTCATA+TGG - chr2_4:10447148-10447167 Msa0314620:intron 45.0%
ACCGAGCTTATTGGACAACA+AGG + chr2_4:10447310-10447329 None:intergenic 45.0%
TGTTGTCCAATAAGCTCGGT+TGG - chr2_4:10447310-10447329 Msa0314620:CDS 45.0%
CACAATCCAACCGAGCTTAT+TGG + chr2_4:10447319-10447338 None:intergenic 45.0%
ATGAAAGGCCATGCTCCAAT+AGG - chr2_4:10447347-10447366 Msa0314620:CDS 45.0%
TATTCCCTCCTATTGGAGCA+TGG + chr2_4:10447358-10447377 None:intergenic 45.0%
ACGATGTTGTGGCAATGAGT+AGG + chr2_4:10447420-10447439 None:intergenic 45.0%
TTCAGGGTTCGCTTATCCAA+TGG - chr2_4:10447456-10447475 Msa0314620:intron 45.0%
! GTTACCTGGGTTAGGCCTAT+TGG - chr2_4:10446958-10446977 Msa0314620:CDS 50.0%
CACTTGGAGTGCATCCCAAT+AGG + chr2_4:10446976-10446995 None:intergenic 50.0%
AAAGGCCATGCTCCAATAGG+AGG - chr2_4:10447350-10447369 Msa0314620:CDS 50.0%
AAGGCCATGCTCCAATAGGA+GGG - chr2_4:10447351-10447370 Msa0314620:CDS 50.0%
!!! TTTTGGGATGCAGTCCATCC+CGG - chr2_4:10447382-10447401 Msa0314620:CDS 50.0%
! GTTACCTGGGTTAGGCCTAT+TGG - chr2_4:10446958-10446977 Msa0314620:CDS 50.0%
CACTTGGAGTGCATCCCAAT+AGG + chr2_4:10446976-10446995 None:intergenic 50.0%
AAAGGCCATGCTCCAATAGG+AGG - chr2_4:10447350-10447369 Msa0314620:CDS 50.0%
AAGGCCATGCTCCAATAGGA+GGG - chr2_4:10447351-10447370 Msa0314620:CDS 50.0%
!!! TTTTGGGATGCAGTCCATCC+CGG - chr2_4:10447382-10447401 Msa0314620:CDS 50.0%
CATCCCAATAGGCCTAACCC+AGG + chr2_4:10446965-10446984 None:intergenic 55.0%
CATCCCAATAGGCCTAACCC+AGG + chr2_4:10446965-10446984 None:intergenic 55.0%
AGTAGGTTCCAAGCCTCGCC+GGG + chr2_4:10447403-10447422 None:intergenic 60.0%
GAGTAGGTTCCAAGCCTCGC+CGG + chr2_4:10447404-10447423 None:intergenic 60.0%
AGTAGGTTCCAAGCCTCGCC+GGG + chr2_4:10447403-10447422 None:intergenic 60.0%
GAGTAGGTTCCAAGCCTCGC+CGG + chr2_4:10447404-10447423 None:intergenic 60.0%
GGATGCAGTCCATCCCGGCG+AGG - chr2_4:10447387-10447406 Msa0314620:CDS 70.0%
CAGTCCATCCCGGCGAGGCT+TGG - chr2_4:10447392-10447411 Msa0314620:intron 70.0%
GGTTCCAAGCCTCGCCGGGA+TGG + chr2_4:10447399-10447418 None:intergenic 70.0%
GGATGCAGTCCATCCCGGCG+AGG - chr2_4:10447387-10447406 Msa0314620:CDS 70.0%
CAGTCCATCCCGGCGAGGCT+TGG - chr2_4:10447392-10447411 Msa0314620:intron 70.0%
GGTTCCAAGCCTCGCCGGGA+TGG + chr2_4:10447399-10447418 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 10446783 10447552 10446783 ID=Msa0314620;Name=Msa0314620
chr2_4 mRNA 10446783 10447552 10446783 ID=Msa0314620-mRNA-1;Parent=Msa0314620;Name=Msa0314620-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|0|0|1|1|1|3|0|180
chr2_4 exon 10447520 10447552 10447520 ID=Msa0314620-mRNA-1:exon:4025;Parent=Msa0314620-mRNA-1
chr2_4 exon 10447166 10447409 10447166 ID=Msa0314620-mRNA-1:exon:4024;Parent=Msa0314620-mRNA-1
chr2_4 exon 10446783 10447048 10446783 ID=Msa0314620-mRNA-1:exon:4023;Parent=Msa0314620-mRNA-1
chr2_4 CDS 10447520 10447552 10447520 ID=Msa0314620-mRNA-1:cds;Parent=Msa0314620-mRNA-1
chr2_4 CDS 10447166 10447409 10447166 ID=Msa0314620-mRNA-1:cds;Parent=Msa0314620-mRNA-1
chr2_4 CDS 10446783 10447048 10446783 ID=Msa0314620-mRNA-1:cds;Parent=Msa0314620-mRNA-1
Gene Sequence

>Msa0314620

TCTCTAATTGAAGAGTTATCATTGAATTTACAGGCTTTACATGATCTTGGAGCAAGAAAATATGTGTTACCTGGGTTAGGCCTATTGGGATGCACTCCAAGTGCCATCCTTACTCATGAGACATATGGGTCTTGTGTTGAGGAGCAAAATGACATTGCATCCATTTTTGATTTCAAGCTTAAATCTTTAGTAGATCACTTCAATAATAAGTTCTCTGCTGATTCCAAATTCATCTTAATAAACAATACATTACAGTCAACTGCCGAGCATTCATATGGTTTTTCGGTTTCAATGGCTCCTTGTTGTCCAATAAGCTCGGTTGGATTGTGTATTCGTGATGAAAGGCCATGCTCCAATAGGAGGGAATATGTGTTTTGGGATGCAGTCCATCCCGGCGAGGCTTGGAACCTACTCATTGCCACAACATCGTACGATTCATCTAATCATTCAGGGTTCGCTTATCCAATGGATATTAAGCACCTTGTTGAACAAGAAATCGAGATGGAATTGGATTTCACTTCACAGCTTAGTGCCTCTGCTTAA
Protein sequence

>Msa0314620

SLIEELSLNLQALHDLGARKYVLPGLGLLGCTPSAILTHETYGSCVEEQNDIASIFDFKLKSLVDHFNNKFSADSKFILINNTLQSTAEHSYGFSVSMAPCCPISSVGLCIRDERPCSNRREYVFWDAVHPGEAWNLLIATTSYDSSNHSGFAYPMDIKHLVEQEIEMELDFTSQLSASA*