AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023817

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023817.t1 MTR_7g076570 91.350 474 32 2 1 474 29 493 0.0 887
MS.gene023817.t1 MTR_7g076760 68.565 474 144 2 1 469 1 474 0.0 662
MS.gene023817.t1 MTR_4g088845 68.697 476 144 2 1 474 1 473 0.0 655
MS.gene023817.t1 MTR_7g050670 67.091 471 152 2 1 469 1 470 0.0 635
MS.gene023817.t1 MTR_1g021825 27.778 216 146 5 1 209 1 213 1.61e-15 79.0
MS.gene023817.t1 MTR_1g021825 27.442 215 146 5 2 209 73 284 7.15e-15 77.0
MS.gene023817.t1 MTR_2g047872 27.386 241 149 7 2 233 8 231 2.33e-14 75.5
MS.gene023817.t1 MTR_0049s0110 21.875 448 294 11 17 448 8 415 2.12e-13 72.4
MS.gene023817.t1 MTR_1g021820 27.477 222 140 8 2 209 11 225 8.46e-12 67.4
MS.gene023817.t1 MTR_4g057725 24.779 226 156 6 2 215 7 230 1.24e-11 67.0
MS.gene023817.t1 MTR_0049s0110 24.561 228 154 5 17 231 8 230 3.21e-11 64.3
MS.gene023817.t1 MTR_1g016340 24.000 275 166 9 4 262 7 254 4.66e-11 65.1
MS.gene023817.t1 MTR_1g016340 24.000 275 166 9 4 262 2 249 5.30e-11 65.1
MS.gene023817.t1 MTR_1g013580 21.839 348 218 11 17 317 19 359 8.71e-11 64.3
MS.gene023817.t1 MTR_0049s0110 24.561 228 154 5 17 231 8 230 9.47e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023817.t1 AT1G61330 29.724 434 295 7 22 446 15 447 1.25e-52 184
MS.gene023817.t1 AT1G61320 26.316 456 318 10 1 446 12 459 2.49e-45 164

Find 61 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGGAGCTTCAATTGTTTA+TGG 0.167841 7.1:+26786865 MS.gene023817:CDS
TTTGCAAAGAGCAACAATTT+TGG 0.207249 7.1:+26787210 MS.gene023817:CDS
TTTCTTCAGGTGATTTGTTT+TGG 0.233766 7.1:-26785782 None:intergenic
CACTCGGAACCTCAGTTAAT+TGG 0.254979 7.1:-26786226 None:intergenic
ACTAAATTCAATTATGAAAC+CGG 0.307435 7.1:-26786150 None:intergenic
ATATTCTCTGGAAGGTTATC+AGG 0.349196 7.1:-26785819 None:intergenic
AGAAACATGAATATGTCATA+TGG 0.394759 7.1:-26786908 None:intergenic
TGGCTGCAAGGATTCGTAAT+GGG 0.404771 7.1:+26786807 MS.gene023817:CDS
TATTTGACCTTGGAATGTAT+TGG 0.411028 7.1:+26787071 MS.gene023817:CDS
TAGAGGTGTAGTTGTGAGAA+TGG 0.421063 7.1:-26786621 None:intergenic
GATGGTAGTAGCAAGTTTGA+AGG 0.422435 7.1:+26787262 MS.gene023817:CDS
ACATGTATATATACCTCAAT+AGG 0.428321 7.1:-26786644 None:intergenic
ACTTGTCAAGTAGAGGTTGC+TGG 0.435147 7.1:-26787102 None:intergenic
CTCCTAGGTACAAAAGTTCA+TGG 0.444404 7.1:+26785904 MS.gene023817:CDS
TTTGTATGAAGCCAACGTTT+TGG 0.444586 7.1:-26787397 None:intergenic
AGCAAAGCTTCGATCCCAAC+GGG 0.449023 7.1:-26786062 None:intergenic
TCGAAGCTTTGCTTAAAAGA+TGG 0.465064 7.1:+26786072 MS.gene023817:CDS
TTGCATCAACCGTGTCTCCT+AGG 0.475524 7.1:+26785889 MS.gene023817:CDS
TCAATTTCCAATACATTCCA+AGG 0.483842 7.1:-26787078 None:intergenic
AGTTACACATCGATCCCGTT+GGG 0.485171 7.1:+26786048 MS.gene023817:CDS
CATTTATTCAGTTCTCATGA+AGG 0.487110 7.1:+26786005 MS.gene023817:CDS
GCGATACAGTCAACAAGATC+TGG 0.487772 7.1:+26785968 MS.gene023817:CDS
AATGATTACATATTTGACCT+TGG 0.490547 7.1:+26787061 MS.gene023817:CDS
GCTTCAATTGTTTATGGATG+AGG 0.491186 7.1:+26786871 MS.gene023817:CDS
ATGAAAAGTTTCACCAAGGA+TGG 0.496691 7.1:-26786941 None:intergenic
GTTACATATATGTACTCAAA+AGG 0.496746 7.1:+26786094 MS.gene023817:CDS
TCTTCCTTCTGAACTTGAGT+TGG 0.498643 7.1:+26787177 MS.gene023817:CDS
TTGCTACTACCATCACTCTC+TGG 0.516294 7.1:-26787253 None:intergenic
ACATATCTTCAGTGATATGA+AGG 0.523594 7.1:-26786283 None:intergenic
TTTGAACGATATTCTCTGGA+AGG 0.540543 7.1:-26785827 None:intergenic
TCAACAAGATCTGGCAGCAA+TGG 0.544368 7.1:+26785977 MS.gene023817:CDS
AACTACACCTCTACCTATTG+AGG 0.545438 7.1:+26786631 MS.gene023817:CDS
GTTCTTGGGCAGCATCGACA+AGG 0.546725 7.1:+26787317 MS.gene023817:CDS
AAGTTACACATCGATCCCGT+TGG 0.549598 7.1:+26786047 MS.gene023817:CDS
GTGGCTGCAAGGATTCGTAA+TGG 0.549630 7.1:+26786806 MS.gene023817:CDS
TGCAGAAATTGCAAACCACT+CGG 0.550616 7.1:-26786242 None:intergenic
TGATTACACTTGTCAAGTAG+AGG 0.553581 7.1:-26787109 None:intergenic
TTTACCAACTCAAGTTCAGA+AGG 0.562421 7.1:-26787181 None:intergenic
CTAAATTCAATTATGAAACC+GGG 0.565228 7.1:-26786149 None:intergenic
ATTCGTAATGGGGTATTACA+AGG 0.565230 7.1:+26786818 MS.gene023817:CDS
TGTGCAATTCGAATTGCTCA+AGG 0.568329 7.1:+26786500 MS.gene023817:CDS
CTTGTTGACTGTATCGCGAA+TGG 0.577500 7.1:-26785962 None:intergenic
TAAAACATGGAACGAAGAGT+TGG 0.579971 7.1:-26786464 None:intergenic
AAGCAAAGCTTCGATCCCAA+CGG 0.588774 7.1:-26786063 None:intergenic
GGCTGCAAGGATTCGTAATG+GGG 0.596055 7.1:+26786808 MS.gene023817:CDS
CTACTACCATCACTCTCTGG+TGG 0.603137 7.1:-26787250 None:intergenic
ATTCAATTATGAAACCGGGT+TGG 0.604950 7.1:-26786145 None:intergenic
ATATATACCTCAATAGGTAG+AGG 0.607892 7.1:-26786638 None:intergenic
AAAATTTCCATGATAAAACA+TGG 0.613851 7.1:-26786477 None:intergenic
GTGCAATTCGAATTGCTCAA+GGG 0.616117 7.1:+26786501 MS.gene023817:CDS
GATTGAATGTAGTGTGTGGA+TGG 0.619503 7.1:-26786557 None:intergenic
TTAATTCCACCAGAGAGTGA+TGG 0.626931 7.1:+26787244 MS.gene023817:CDS
ATCCAACCCATCCAAAACGT+TGG 0.629640 7.1:+26787386 MS.gene023817:CDS
TTGTGCGATCCAATTAACTG+AGG 0.637065 7.1:+26786217 MS.gene023817:CDS
GAGTTGCATCTTTACCAACC+CGG 0.641242 7.1:+26786131 MS.gene023817:CDS
ATCAATGAAAAGTTTCACCA+AGG 0.652727 7.1:-26786945 None:intergenic
CAATTAACTGAGGTTCCGAG+TGG 0.669011 7.1:+26786227 MS.gene023817:CDS
TTCTTGGGCAGCATCGACAA+GGG 0.669900 7.1:+26787318 MS.gene023817:CDS
TCAAGGGATGAAATTGTACG+AGG 0.699708 7.1:+26786517 MS.gene023817:CDS
TCTTCGACTAGAGCTTCGAG+CGG 0.699852 7.1:-26786584 None:intergenic
AGTAGATTGAATGTAGTGTG+TGG 0.702903 7.1:-26786561 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTAAACCTAATAATTTTA+GGG + chr7.1:26786782-26786801 MS.gene023817:intron 10.0%
!!! TTATTAAACCTAATAATTTT+AGG + chr7.1:26786781-26786800 MS.gene023817:intron 10.0%
!!! ATTACAATTGCATTTTTTAT+TGG - chr7.1:26785870-26785889 None:intergenic 15.0%
!! AAAATTTCCATGATAAAACA+TGG - chr7.1:26786480-26786499 None:intergenic 20.0%
!! ACTAAATTCAATTATGAAAC+CGG - chr7.1:26786153-26786172 None:intergenic 20.0%
!! CAAGTTAAAAACACTAAAAT+TGG + chr7.1:26786190-26786209 MS.gene023817:CDS 20.0%
!!! ATATTAATAAAGGCATCAAA+TGG - chr7.1:26786754-26786773 None:intergenic 20.0%
!!! ATTTCAATCTTTTTCAAGTT+TGG - chr7.1:26786437-26786456 None:intergenic 20.0%
!!! GCAAAATATGTAAAACTTTT+TGG + chr7.1:26787295-26787314 MS.gene023817:CDS 20.0%
! AATGATTACATATTTGACCT+TGG + chr7.1:26787061-26787080 MS.gene023817:CDS 25.0%
! ACATGTATATATACCTCAAT+AGG - chr7.1:26786647-26786666 None:intergenic 25.0%
! AGAAACATGAATATGTCATA+TGG - chr7.1:26786911-26786930 None:intergenic 25.0%
! CTAAATTCAATTATGAAACC+GGG - chr7.1:26786152-26786171 None:intergenic 25.0%
! GTTACATATATGTACTCAAA+AGG + chr7.1:26786094-26786113 MS.gene023817:CDS 25.0%
! TTCATTGATGTGAGTAATAT+TGG + chr7.1:26786959-26786978 MS.gene023817:intron 25.0%
!!! ATATTGTTTTGAGAATCTGT+TGG + chr7.1:26786847-26786866 MS.gene023817:CDS 25.0%
!!! ATGTAAAACTTTTTGGTTCT+TGG + chr7.1:26787302-26787321 MS.gene023817:CDS 25.0%
!!! ATTTTTTGAACGATATTCTC+TGG - chr7.1:26785834-26785853 None:intergenic 25.0%
!!! CAATTGCATTTTTTATTGGT+AGG - chr7.1:26785866-26785885 None:intergenic 25.0%
!!! GAATCAGTTTTATTTTCTTC+AGG - chr7.1:26785798-26785817 None:intergenic 25.0%
!!! TGTAAAACTTTTTGGTTCTT+GGG + chr7.1:26787303-26787322 MS.gene023817:CDS 25.0%
ACATATCTTCAGTGATATGA+AGG - chr7.1:26786286-26786305 None:intergenic 30.0%
ATATATACCTCAATAGGTAG+AGG - chr7.1:26786641-26786660 None:intergenic 30.0%
ATCAATGAAAAGTTTCACCA+AGG - chr7.1:26786948-26786967 None:intergenic 30.0%
CATTTATTCAGTTCTCATGA+AGG + chr7.1:26786005-26786024 MS.gene023817:CDS 30.0%
TATTTGACCTTGGAATGTAT+TGG + chr7.1:26787071-26787090 MS.gene023817:CDS 30.0%
TCAATTTCCAATACATTCCA+AGG - chr7.1:26787081-26787100 None:intergenic 30.0%
! TTTGCAAAGAGCAACAATTT+TGG + chr7.1:26787210-26787229 MS.gene023817:CDS 30.0%
!!! AACCTAATAATTTTAGGGTG+TGG + chr7.1:26786787-26786806 MS.gene023817:intron 30.0%
!!! TTTCTTCAGGTGATTTGTTT+TGG - chr7.1:26785785-26785804 None:intergenic 30.0%
AGCCACACCCTAAAATTATT+AGG - chr7.1:26786792-26786811 None:intergenic 35.0%
AGTAGATTGAATGTAGTGTG+TGG - chr7.1:26786564-26786583 None:intergenic 35.0%
ATGAAAAGTTTCACCAAGGA+TGG - chr7.1:26786944-26786963 None:intergenic 35.0%
ATTCAATTATGAAACCGGGT+TGG - chr7.1:26786148-26786167 None:intergenic 35.0%
GCTTCAATTGTTTATGGATG+AGG + chr7.1:26786871-26786890 MS.gene023817:CDS 35.0%
GTTGGAGCTTCAATTGTTTA+TGG + chr7.1:26786865-26786884 MS.gene023817:CDS 35.0%
TAAAACATGGAACGAAGAGT+TGG - chr7.1:26786467-26786486 None:intergenic 35.0%
TCGAAGCTTTGCTTAAAAGA+TGG + chr7.1:26786072-26786091 MS.gene023817:CDS 35.0%
TCTCAAAAATTGTCCATCCT+TGG + chr7.1:26786928-26786947 MS.gene023817:CDS 35.0%
TGATTACACTTGTCAAGTAG+AGG - chr7.1:26787112-26787131 None:intergenic 35.0%
TTTACCAACTCAAGTTCAGA+AGG - chr7.1:26787184-26787203 None:intergenic 35.0%
TTTGAACGATATTCTCTGGA+AGG - chr7.1:26785830-26785849 None:intergenic 35.0%
! ATATTCTCTGGAAGGTTATC+AGG - chr7.1:26785822-26785841 None:intergenic 35.0%
! ATTCGTAATGGGGTATTACA+AGG + chr7.1:26786818-26786837 MS.gene023817:CDS 35.0%
! CTTCGTTCCATGTTTTATCA+TGG + chr7.1:26786470-26786489 MS.gene023817:CDS 35.0%
! TTTGTATGAAGCCAACGTTT+TGG - chr7.1:26787400-26787419 None:intergenic 35.0%
!! AAAGAGCAACAATTTTGGAG+AGG + chr7.1:26787215-26787234 MS.gene023817:CDS 35.0%
AACTACACCTCTACCTATTG+AGG + chr7.1:26786631-26786650 MS.gene023817:CDS 40.0%
CTCCTAGGTACAAAAGTTCA+TGG + chr7.1:26785904-26785923 MS.gene023817:CDS 40.0%
GATGGTAGTAGCAAGTTTGA+AGG + chr7.1:26787262-26787281 MS.gene023817:CDS 40.0%
GATTGAATGTAGTGTGTGGA+TGG - chr7.1:26786560-26786579 None:intergenic 40.0%
GTGCAATTCGAATTGCTCAA+GGG + chr7.1:26786501-26786520 MS.gene023817:CDS 40.0%
TCAAGGGATGAAATTGTACG+AGG + chr7.1:26786517-26786536 MS.gene023817:CDS 40.0%
TCTTCCTTCTGAACTTGAGT+TGG + chr7.1:26787177-26787196 MS.gene023817:CDS 40.0%
TGCAGAAATTGCAAACCACT+CGG - chr7.1:26786245-26786264 None:intergenic 40.0%
TGTGCAATTCGAATTGCTCA+AGG + chr7.1:26786500-26786519 MS.gene023817:CDS 40.0%
TTAATTCCACCAGAGAGTGA+TGG + chr7.1:26787244-26787263 MS.gene023817:CDS 40.0%
TTGTGCGATCCAATTAACTG+AGG + chr7.1:26786217-26786236 MS.gene023817:CDS 40.0%
! ACTTTTGTACCTAGGAGACA+CGG - chr7.1:26785901-26785920 None:intergenic 40.0%
! TAGAGGTGTAGTTGTGAGAA+TGG - chr7.1:26786624-26786643 None:intergenic 40.0%
!! ATGAAGCCAACGTTTTGGAT+GGG - chr7.1:26787395-26787414 None:intergenic 40.0%
!! TATGAAGCCAACGTTTTGGA+TGG - chr7.1:26787396-26787415 None:intergenic 40.0%
AAGCAAAGCTTCGATCCCAA+CGG - chr7.1:26786066-26786085 None:intergenic 45.0%
AAGTTACACATCGATCCCGT+TGG + chr7.1:26786047-26786066 MS.gene023817:CDS 45.0%
ACTTGTCAAGTAGAGGTTGC+TGG - chr7.1:26787105-26787124 None:intergenic 45.0%
AGTTACACATCGATCCCGTT+GGG + chr7.1:26786048-26786067 MS.gene023817:CDS 45.0%
ATCCAACCCATCCAAAACGT+TGG + chr7.1:26787386-26787405 MS.gene023817:CDS 45.0%
CAATTAACTGAGGTTCCGAG+TGG + chr7.1:26786227-26786246 MS.gene023817:CDS 45.0%
CACTCGGAACCTCAGTTAAT+TGG - chr7.1:26786229-26786248 None:intergenic 45.0%
GAGTTGCATCTTTACCAACC+CGG + chr7.1:26786131-26786150 MS.gene023817:CDS 45.0%
GCGATACAGTCAACAAGATC+TGG + chr7.1:26785968-26785987 MS.gene023817:CDS 45.0%
TCAACAAGATCTGGCAGCAA+TGG + chr7.1:26785977-26785996 MS.gene023817:CDS 45.0%
TGGCTGCAAGGATTCGTAAT+GGG + chr7.1:26786807-26786826 MS.gene023817:CDS 45.0%
TTGCTACTACCATCACTCTC+TGG - chr7.1:26787256-26787275 None:intergenic 45.0%
! CGCCATGAACTTTTGTACCT+AGG - chr7.1:26785909-26785928 None:intergenic 45.0%
! CTTGTTGACTGTATCGCGAA+TGG - chr7.1:26785965-26785984 None:intergenic 45.0%
!!! AATTTTAGGGTGTGGCTGCA+AGG + chr7.1:26786795-26786814 MS.gene023817:intron 45.0%
!! TAATTTATACATATTAATAA+AGG - chr7.1:26786764-26786783 None:intergenic 5.0%
AGCAAAGCTTCGATCCCAAC+GGG - chr7.1:26786065-26786084 None:intergenic 50.0%
CTACTACCATCACTCTCTGG+TGG - chr7.1:26787253-26787272 None:intergenic 50.0%
GGCTGCAAGGATTCGTAATG+GGG + chr7.1:26786808-26786827 MS.gene023817:CDS 50.0%
GTGGCTGCAAGGATTCGTAA+TGG + chr7.1:26786806-26786825 MS.gene023817:CDS 50.0%
TCTTCGACTAGAGCTTCGAG+CGG - chr7.1:26786587-26786606 None:intergenic 50.0%
TTGCATCAACCGTGTCTCCT+AGG + chr7.1:26785889-26785908 MS.gene023817:CDS 50.0%
!! AGCCAACGTTTTGGATGGGT+TGG - chr7.1:26787391-26787410 None:intergenic 50.0%
!! TTCTTGGGCAGCATCGACAA+GGG + chr7.1:26787318-26787337 MS.gene023817:CDS 50.0%
!! GTTCTTGGGCAGCATCGACA+AGG + chr7.1:26787317-26787336 MS.gene023817:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 26785765 26787429 26785765 ID=MS.gene023817
chr7.1 mRNA 26785765 26787429 26785765 ID=MS.gene023817.t1;Parent=MS.gene023817
chr7.1 exon 26785765 26786652 26785765 ID=MS.gene023817.t1.exon1;Parent=MS.gene023817.t1
chr7.1 CDS 26785765 26786652 26785765 ID=cds.MS.gene023817.t1;Parent=MS.gene023817.t1
chr7.1 exon 26786803 26786967 26786803 ID=MS.gene023817.t1.exon2;Parent=MS.gene023817.t1
chr7.1 CDS 26786803 26786967 26786803 ID=cds.MS.gene023817.t1;Parent=MS.gene023817.t1
chr7.1 exon 26787058 26787429 26787058 ID=MS.gene023817.t1.exon3;Parent=MS.gene023817.t1
chr7.1 CDS 26787058 26787429 26787058 ID=cds.MS.gene023817.t1;Parent=MS.gene023817.t1
Gene Sequence

>MS.gene023817

ATGAAAAATTCTGAAAACCAAAACAAATCACCTGAAGAAAATAAAACTGATTCACCTGATAACCTTCCAGAGAATATCGTTCAAAAAATTTTCACTTTCCTACCAATAAAAAATGCAATTGTAATTGCATCAACCGTGTCTCCTAGGTACAAAAGTTCATGGCGTCATAATCGTCGATTCTTGTTTGATAGTGACTTCCATTCGCGATACAGTCAACAAGATCTGGCAGCAATGGTTGATCATTTATTCAGTTCTCATGAAGGTGACGAAATCAAAACATTTAAGTTACACATCGATCCCGTTGGGATCGAAGCTTTGCTTAAAAGATGGTTACATATATGTACTCAAAAGGATCTAGAAGATCTTGAGTTGCATCTTTACCAACCCGGTTTCATAATTGAATTTAGTGTTTTTAACGCACTTCACAAGTTAAAAACACTAAAATTGGTTAATTGTGCGATCCAATTAACTGAGGTTCCGAGTGGTTTGCAATTTCTGCAAACACTAAGTCTATGTAACCTTCATATCACTGAAGATATGTTTGATGTGTTGATTGAGCATTGTAAAATGCTTGAAATTATTGATTTGATTAATTGCTCTACAATTAATAATCTCAACTTAAATGCTAGAGAAAATAAACATCTCAAAAAATTGAGACTTGTTGAATGTCCAAACTTGAAAAAGATTGAAATTGATTCACCAACTCTTCGTTCCATGTTTTATCATGGAAATTTTTGTGCAATTCGAATTGCTCAAGGGATGAAATTGTACGAGGCTTTCTTTTACTTTATACCATCCACACACTACATTCAATCTACTCCGCTCGAAGCTCTAGTCGAAGATCTCTCTAATGTTTCCATTCTCACAACTACACCTCTACCTATTGAGGTATATATACATGTTTAATATACTTATTTACATTTAATTCTTTACTATTTAATTTAATTTCGTAAAGATTATAGCATGTTATACATTTATTATTATCACCATTTGATGCCTTTATTAATATGTATAAATTATTAAACCTAATAATTTTAGGGTGTGGCTGCAAGGATTCGTAATGGGGTATTACAAGGAGCTCAATATTGTTTTGAGAATCTGTTGGAGCTTCAATTGTTTATGGATGAGGCAGTGTTTTGCAATCCATATGACATATTCATGTTTCTCAAAAATTGTCCATCCTTGGTGAAACTTTTCATTGATGTGAGTAATATTGGAGCATACTTTTGATCTCTCGAGTAATTTTTTTTTTTTTTTTTTTGTCTTGAGTCTAACTTTAAATTATATATGCAGCTAAATGATTACATATTTGACCTTGGAATGTATTGGAAATTGAACCAGCAACCTCTACTTGACAAGTGTAATCACAAATTTACTCAGCTCAAAGTTCTGATGATAAGAAATTTTAAGTTTCTTCCTTCTGAACTTGAGTTGGTAAAGATAGTTTTGCAAAGAGCAACAATTTTGGAGAGGTTGACTTTAATTCCACCAGAGAGTGATGGTAGTAGCAAGTTTGAAGGAGAATATGCAGCAAAATATGTAAAACTTTTTGGTTCTTGGGCAGCATCGACAAGGGTCATAATAAAGTTACATGAAAAGTACGTTGATAAGAGTTTTGACAATCCAACCCATCCAAAACGTTGGCTTCATACAAATGCAAATTGA
Protein sequence

>MS.gene023817.t1

MKNSENQNKSPEENKTDSPDNLPENIVQKIFTFLPIKNAIVIASTVSPRYKSSWRHNRRFLFDSDFHSRYSQQDLAAMVDHLFSSHEGDEIKTFKLHIDPVGIEALLKRWLHICTQKDLEDLELHLYQPGFIIEFSVFNALHKLKTLKLVNCAIQLTEVPSGLQFLQTLSLCNLHITEDMFDVLIEHCKMLEIIDLINCSTINNLNLNARENKHLKKLRLVECPNLKKIEIDSPTLRSMFYHGNFCAIRIAQGMKLYEAFFYFIPSTHYIQSTPLEALVEDLSNVSILTTTPLPIEGVAARIRNGVLQGAQYCFENLLELQLFMDEAVFCNPYDIFMFLKNCPSLVKLFIDLNDYIFDLGMYWKLNQQPLLDKCNHKFTQLKVLMIRNFKFLPSELELVKIVLQRATILERLTLIPPESDGSSKFEGEYAAKYVKLFGSWAASTRVIIKLHEKYVDKSFDNPTHPKRWLHTNAN