AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34740.t1 MTR_4g050250 52.471 425 155 8 9 401 8 417 4.35e-133 388
MS.gene34740.t1 MTR_8g469020 53.086 405 148 9 23 401 6 394 4.14e-128 377
MS.gene34740.t1 MTR_8g058965 52.771 415 153 10 12 401 3 399 4.00e-122 362
MS.gene34740.t1 MTR_8g062010 51.282 390 153 8 24 401 9 373 3.41e-115 343
MS.gene34740.t1 MTR_8g070950 51.435 418 145 15 1 401 1 377 1.99e-111 333
MS.gene34740.t1 MTR_8g062300 47.887 426 173 13 9 401 11 420 4.10e-105 319
MS.gene34740.t1 MTR_8g062080 48.849 391 164 10 23 401 22 388 9.89e-100 304
MS.gene34740.t1 MTR_8g062310 48.705 386 157 10 21 401 22 371 4.11e-99 302
MS.gene34740.t1 MTR_8g467790 46.310 393 146 14 24 400 148 491 3.73e-94 291
MS.gene34740.t1 MTR_8g468810 42.105 418 209 15 1 400 1 403 6.53e-81 256
MS.gene34740.t1 MTR_8g468810 42.188 384 192 13 1 367 1 371 2.66e-73 233
MS.gene34740.t1 MTR_8g468810 46.885 305 139 11 1 288 1 299 3.90e-67 216
MS.gene34740.t1 MTR_8g468810 46.885 305 139 11 1 288 1 299 7.32e-67 216
MS.gene34740.t1 MTR_4g032730 37.237 427 214 10 12 401 3 412 9.32e-67 219
MS.gene34740.t1 MTR_8g062270 39.706 408 191 14 24 399 16 400 1.20e-66 219
MS.gene34740.t1 MTR_8g468810 46.885 305 139 11 1 288 1 299 2.03e-66 215
MS.gene34740.t1 MTR_2g083840 38.517 418 220 10 1 401 1 398 7.16e-66 217
MS.gene34740.t1 MTR_8g058970 38.128 438 211 18 3 401 2 418 5.36e-62 207
MS.gene34740.t1 MTR_8g445780 38.971 408 197 17 13 399 2 378 7.63e-58 194
MS.gene34740.t1 MTR_8g468840 35.610 410 225 10 13 400 3 395 5.18e-55 188
MS.gene34740.t1 MTR_8g467800 36.364 418 214 14 10 401 8 399 1.07e-54 187
MS.gene34740.t1 MTR_5g092960 36.277 419 209 13 15 400 14 407 4.43e-53 183
MS.gene34740.t1 MTR_8g014260 33.803 426 223 15 1 400 1 393 1.30e-47 168
MS.gene34740.t1 MTR_8g468760 35.663 415 185 14 13 400 2 361 1.37e-47 167
MS.gene34740.t1 MTR_5g092960 35.509 383 192 12 15 367 14 371 2.23e-43 156
MS.gene34740.t1 MTR_8g467800 36.825 315 166 12 10 304 8 309 2.23e-35 133
MS.gene34740.t1 MTR_8g014160 32.346 405 187 16 17 401 32 369 2.68e-35 135
MS.gene34740.t1 MTR_2g086020 59.649 114 43 2 290 401 146 258 3.83e-33 126
MS.gene34740.t1 MTR_2g086020 49.673 153 62 6 50 196 1 144 2.46e-32 124
MS.gene34740.t1 MTR_5g021550 49.342 152 71 5 18 165 13 162 1.00e-32 121
MS.gene34740.t1 MTR_8g077460 38.991 218 124 5 22 231 8 224 3.95e-28 114
MS.gene34740.t1 MTR_8g014290 45.161 155 73 6 22 166 8 160 8.65e-25 99.8
MS.gene34740.t1 MTR_4g024720 41.885 191 76 9 1 168 1 179 8.65e-23 95.9
MS.gene34740.t1 MTR_3g023630 34.872 195 113 9 20 210 4 188 9.55e-19 87.4
MS.gene34740.t1 MTR_7g032380 34.500 200 111 8 19 211 6 192 1.52e-18 86.7
MS.gene34740.t1 MTR_3g107100 35.176 199 106 9 22 211 9 193 2.34e-17 83.6
MS.gene34740.t1 MTR_3g105060 30.808 198 120 8 19 211 9 194 1.87e-15 77.8
MS.gene34740.t1 MTR_8g085430 32.995 197 117 8 23 211 10 199 1.96e-15 77.0
MS.gene34740.t1 MTR_1g041245 30.500 200 117 8 19 210 6 191 2.58e-15 77.4
MS.gene34740.t1 MTR_7g033945 32.000 175 108 5 19 190 6 172 4.50e-15 73.2
MS.gene34740.t1 MTR_7g056447 32.489 237 131 12 24 250 9 226 6.94e-15 75.1
MS.gene34740.t1 MTR_4g024745 32.911 237 129 10 178 401 26 245 1.21e-14 74.3
MS.gene34740.t1 MTR_2g007450 33.333 210 114 9 21 210 13 216 1.35e-14 75.5
MS.gene34740.t1 MTR_7g115270 30.732 205 126 7 16 211 4 201 1.40e-14 75.1
MS.gene34740.t1 MTR_7g114710 32.474 194 113 9 24 211 13 194 1.40e-14 75.1
MS.gene34740.t1 MTR_2g007500 32.836 201 102 8 20 211 10 186 2.52e-14 73.9
MS.gene34740.t1 MTR_7g055943 29.231 195 123 6 21 210 37 221 3.38e-14 74.3
MS.gene34740.t1 MTR_7g033990 33.663 202 111 8 20 211 7 195 6.47e-14 73.2
MS.gene34740.t1 MTR_7g098510 31.163 215 116 9 21 221 15 211 7.74e-14 72.8
MS.gene34740.t1 MTR_2g007400 31.925 213 122 9 24 220 12 217 1.07e-13 72.4
MS.gene34740.t1 MTR_5g073420 32.308 195 114 9 24 210 11 195 1.55e-13 72.0
MS.gene34740.t1 MTR_4g134150 31.937 191 108 7 24 210 14 186 2.06e-13 69.7
MS.gene34740.t1 MTR_1g108820 34.197 193 107 10 23 210 7 184 2.65e-13 71.2
MS.gene34740.t1 MTR_1g108820 34.197 193 107 10 23 210 7 184 7.43e-13 69.7
MS.gene34740.t1 MTR_7g033915 26.573 286 154 11 20 299 7 242 7.71e-13 69.7
MS.gene34740.t1 MTR_4g024770 50.685 73 28 2 1 73 8 72 8.96e-13 63.9
MS.gene34740.t1 MTR_7g034115 29.707 239 129 10 23 239 10 231 8.99e-13 69.7
MS.gene34740.t1 MTR_3g107120 35.238 210 109 12 18 210 3 202 2.40e-12 68.6
MS.gene34740.t1 MTR_3g107160 29.648 199 122 6 24 214 10 198 8.54e-12 66.6
MS.gene34740.t1 MTR_5g058100 31.220 205 121 8 17 214 2 193 9.32e-12 66.6
MS.gene34740.t1 MTR_4g098590 30.303 198 114 7 29 211 4 192 9.65e-12 66.2
MS.gene34740.t1 MTR_5g083890 31.579 209 109 11 24 208 6 204 1.01e-11 67.0
MS.gene34740.t1 MTR_7g098570 33.498 203 109 10 23 214 19 206 1.33e-11 66.2
MS.gene34740.t1 MTR_3g108150 30.612 196 117 7 20 211 9 189 1.35e-11 65.9
MS.gene34740.t1 MTR_4g093690 30.653 199 111 7 22 208 16 199 1.51e-11 65.1
MS.gene34740.t1 MTR_4g113810 35.714 112 69 1 121 229 19 130 2.02e-11 62.4
MS.gene34740.t1 MTR_5g080710 25.888 394 246 14 24 401 33 396 2.62e-11 65.5
MS.gene34740.t1 MTR_7g098480 33.333 204 103 9 21 211 15 198 2.84e-11 65.5
MS.gene34740.t1 MTR_3g082890 28.807 243 134 9 5 224 78 304 3.70e-11 65.1
MS.gene34740.t1 MTR_8g014210 32.828 198 93 9 220 401 2 175 4.71e-11 62.8
MS.gene34740.t1 MTR_7g056433 35.025 197 104 11 24 210 12 194 5.08e-11 64.3
MS.gene34740.t1 MTR_3g082890 28.452 239 134 8 8 224 1 224 7.21e-11 63.9
MS.gene34740.t1 MTR_7g034110 30.151 199 121 7 20 211 7 194 7.73e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34740.t1 AT4G10400 34.211 190 113 6 24 210 2 182 2.02e-15 77.8
MS.gene34740.t1 AT4G10400 34.211 190 113 6 24 210 2 182 2.02e-15 77.8
MS.gene34740.t1 AT4G10400 34.211 190 113 6 24 210 2 182 2.02e-15 77.8
MS.gene34740.t1 AT4G09920 33.684 190 114 6 24 210 2 182 9.59e-15 75.1
MS.gene34740.t1 AT4G09920 33.684 190 114 6 24 210 2 182 9.59e-15 75.1
MS.gene34740.t1 AT4G09920 33.684 190 114 6 24 210 2 182 1.53e-14 73.9
MS.gene34740.t1 AT4G09920 33.684 190 114 6 24 210 2 182 1.53e-14 73.9
MS.gene34740.t1 AT4G09920 33.684 190 114 6 24 210 2 182 1.53e-14 73.9
MS.gene34740.t1 AT1G16930 30.208 192 120 6 22 210 13 193 2.23e-14 75.1
MS.gene34740.t1 AT1G16930 30.208 192 120 6 22 210 13 193 2.23e-14 75.1
MS.gene34740.t1 AT1G16930 30.208 192 120 6 22 210 13 193 2.23e-14 75.1
MS.gene34740.t1 AT4G00160 33.480 227 125 8 20 234 12 224 6.50e-14 73.6
MS.gene34740.t1 AT3G58930 29.000 200 124 6 24 210 2 196 6.89e-14 73.6
MS.gene34740.t1 AT3G58930 29.000 200 124 6 24 210 2 196 6.89e-14 73.6
MS.gene34740.t1 AT3G58930 29.000 200 124 6 24 210 2 196 6.89e-14 73.6
MS.gene34740.t1 AT5G56440 32.990 194 110 7 24 210 2 182 2.12e-13 72.0
MS.gene34740.t1 AT5G44980 26.065 399 250 15 22 398 2 377 2.14e-12 68.9
MS.gene34740.t1 AT5G44980 26.065 399 250 15 22 398 2 377 2.14e-12 68.9
MS.gene34740.t1 AT3G58940 29.851 201 122 7 24 210 2 197 2.67e-12 68.9
MS.gene34740.t1 AT3G58940 29.851 201 122 7 24 210 2 197 2.67e-12 68.9
MS.gene34740.t1 AT5G41630 29.703 202 119 7 24 210 12 205 1.40e-11 66.2
MS.gene34740.t1 AT3G60040 25.195 385 229 16 17 364 2 364 3.02e-11 65.5
MS.gene34740.t1 AT3G60040 25.195 385 229 16 17 364 221 583 4.62e-11 65.1
MS.gene34740.t1 AT3G60040 25.195 385 229 16 17 364 221 583 4.84e-11 65.1
MS.gene34740.t1 AT3G60040 25.195 385 229 16 17 364 222 584 4.89e-11 65.1
MS.gene34740.t1 AT3G60040 25.195 385 229 16 17 364 219 581 4.95e-11 65.1
MS.gene34740.t1 AT1G66290 24.010 404 263 14 13 398 18 395 5.06e-11 64.7

Find 105 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATCATCATCTTCTTCTTC+TGG 0.190605 8.1:+44855407 None:intergenic
TCAATTGTTTCCTTTCTATT+TGG 0.203600 8.1:-44854335 MS.gene34740:CDS
CATGCATTTGTTTGAGTATA+GGG 0.228369 8.1:+44854078 None:intergenic
GCGTACCTTCTGAATATTTC+AGG 0.244532 8.1:+44855203 None:intergenic
TTATGTAAGTTCTGAAATTC+TGG 0.245998 8.1:+44853896 None:intergenic
AGATGGGATTCGAGACATTT+AGG 0.262067 8.1:+44853581 None:intergenic
GCAGTCTTGTTGGTGATATC+TGG 0.293056 8.1:-44854427 MS.gene34740:intron
TGCAGGTCCCATGTTCTTAA+AGG 0.312968 8.1:+44853617 None:intergenic
ATGCCCCATGCTTGAAACTT+TGG 0.377727 8.1:-44854312 MS.gene34740:CDS
TTACAATGTCGCGGGAATTA+CGG 0.378805 8.1:+44855153 None:intergenic
CGTGCTTTGTATCTATTTGA+AGG 0.385118 8.1:+44854204 None:intergenic
GAGTTTGATTCTGATGGAAC+TGG 0.387587 8.1:-44855023 MS.gene34740:CDS
TCATGCATTTGTTTGAGTAT+AGG 0.401533 8.1:+44854077 None:intergenic
TGAATACACTGCAGTCTTGT+TGG 0.409001 8.1:-44854437 MS.gene34740:intron
GAAATTCCCATTGAACCTCT+TGG 0.414884 8.1:+44854238 None:intergenic
CTGAACTATATTCACATAGA+AGG 0.415568 8.1:-44853560 MS.gene34740:CDS
CTCCCAGGTATCTTCATCTC+TGG 0.418540 8.1:-44855277 MS.gene34740:CDS
AAGTGGGTCCGTGCTGCCAT+TGG 0.421115 8.1:-44855071 MS.gene34740:CDS
CCTTCCTCCAAGAGGTTCAA+TGG 0.424571 8.1:-44854245 MS.gene34740:CDS
GGTTCAATGGGAATTTCTCT+TGG 0.425278 8.1:-44854232 MS.gene34740:CDS
AGTGGGTCCGTGCTGCCATT+GGG 0.436081 8.1:-44855070 MS.gene34740:CDS
ATGCTTCAACTCTAATCTTC+TGG 0.444405 8.1:-44853855 MS.gene34740:CDS
TTATAAGAACTTCAAGCATA+TGG 0.454068 8.1:+44853813 None:intergenic
CATGTTCTTAAAGGTGATGA+TGG 0.454851 8.1:+44853626 None:intergenic
TAAATATAGTTCTTGAAGGT+GGG 0.461382 8.1:+44855046 None:intergenic
GCACCAATCTTGTTTCTCTC+AGG 0.462896 8.1:-44854962 MS.gene34740:intron
CTCTTGGAGGAAGGTGGCAT+TGG 0.463450 8.1:+44854254 None:intergenic
ATTCTGGATGATTTAGAGCC+GGG 0.467016 8.1:+44853912 None:intergenic
TATTTAGAGTTTGATTCTGA+TGG 0.470671 8.1:-44855029 MS.gene34740:CDS
CTTCCAGAGATGAAGATACC+TGG 0.483060 8.1:+44855274 None:intergenic
TAGGAACTGTTGTTGACTGC+AGG 0.484641 8.1:+44853600 None:intergenic
CTTTGTATCTATTTGAAGGC+AGG 0.487118 8.1:+44854208 None:intergenic
CTTCCTCCAAGAGGTTCAAT+GGG 0.490516 8.1:-44854244 MS.gene34740:CDS
ACTCTAAATATAGTTCTTGA+AGG 0.491238 8.1:+44855042 None:intergenic
TTCTTGAAGGTGGGGCCCAA+TGG 0.493823 8.1:+44855055 None:intergenic
TCATCTCTGGAAGGATCTAC+AGG 0.496046 8.1:-44855264 MS.gene34740:CDS
ATTGCTTTGAGTTCAGTCCT+AGG 0.497225 8.1:-44854286 MS.gene34740:CDS
TTGGTAGGAAGGACATGATA+TGG 0.497535 8.1:+44855333 None:intergenic
GTTGGGCATAATCGGCAACT+TGG 0.506137 8.1:-44854159 MS.gene34740:CDS
AGTTTCAACATCTTCAACAA+TGG 0.509123 8.1:+44854377 None:intergenic
ATTCACATAGAAGGATATGA+AGG 0.511071 8.1:-44853551 MS.gene34740:CDS
ACGAGATAACACCGCTGAGA+TGG 0.514490 8.1:+44855126 None:intergenic
CATCATCACCTTTAAGAACA+TGG 0.516525 8.1:-44853625 MS.gene34740:CDS
TTCGTCGTCGTAAGTGTCCT+CGG 0.516608 8.1:+44855229 None:intergenic
CTATGTGAATATAGTTCAGA+TGG 0.517466 8.1:+44853564 None:intergenic
TTGGTGCAATTCAAGATAGA+AGG 0.518435 8.1:+44854978 None:intergenic
GGCATTGGTACTTCGTTCCT+AGG 0.519756 8.1:+44854269 None:intergenic
CAGAAGGTACGCTTTATATG+TGG 0.521026 8.1:-44855192 MS.gene34740:CDS
TGATCCAAAGTTTCAAGCAT+GGG 0.524411 8.1:+44854308 None:intergenic
ATATGGCACAGGAGTGAGTC+CGG 0.525026 8.1:+44855350 None:intergenic
GACGAGGCTCATGGTGGCCA+CGG 0.525721 8.1:+44855301 None:intergenic
AATTCTGGATGATTTAGAGC+CGG 0.535727 8.1:+44853911 None:intergenic
GCAACGAATACATAGATAAG+TGG 0.539029 8.1:-44855088 MS.gene34740:CDS
CTAAATATAGTTCTTGAAGG+TGG 0.540820 8.1:+44855045 None:intergenic
AGAAGATTAGAGTTGAAGCA+TGG 0.543413 8.1:+44853857 None:intergenic
CATGGGGCATCCAAATAGAA+AGG 0.550294 8.1:+44854325 None:intergenic
CCATGAGCCTCGTCTCTCCC+AGG 0.554497 8.1:-44855292 MS.gene34740:CDS
AACAAGTGATGGCTTAGTAC+AGG 0.556375 8.1:+44853964 None:intergenic
GAAGATTAGAGTTGAAGCAT+GGG 0.556638 8.1:+44853858 None:intergenic
CTACCTGAGAGAAACAAGAT+TGG 0.557491 8.1:+44854959 None:intergenic
CCATTGAACCTCTTGGAGGA+AGG 0.561236 8.1:+44854245 None:intergenic
AAATATAGTTCTTGAAGGTG+GGG 0.564805 8.1:+44855047 None:intergenic
CCAGAAGAAGAAGATGATGA+TGG 0.564861 8.1:-44855407 MS.gene34740:CDS
ATTCCCATTGAACCTCTTGG+AGG 0.569474 8.1:+44854241 None:intergenic
GGTGTTATCTCGTATGATCA+TGG 0.577716 8.1:-44855116 MS.gene34740:CDS
CGACAGATTGAGCATTCTAC+CGG 0.583446 8.1:-44855369 MS.gene34740:CDS
ATGGAACTTTACAATGTCGC+GGG 0.584776 8.1:+44855145 None:intergenic
GCAGTGGCCACTTCATGCCC+CGG 0.585810 8.1:-44853930 MS.gene34740:CDS
TTCCAGAGATGAAGATACCT+GGG 0.587843 8.1:+44855275 None:intergenic
GGAAGGACATGATATGGCAC+AGG 0.590863 8.1:+44855339 None:intergenic
ATCATCACCTTTAAGAACAT+GGG 0.593418 8.1:-44853624 MS.gene34740:CDS
ATGATCCAAAGTTTCAAGCA+TGG 0.594214 8.1:+44854307 None:intergenic
TTCTGGATGATTTAGAGCCG+GGG 0.594254 8.1:+44853913 None:intergenic
CCTGGGAGAGACGAGGCTCA+TGG 0.595342 8.1:+44855292 None:intergenic
CGGCAACTTGGAGAGTGTGA+TGG 0.595564 8.1:-44854147 MS.gene34740:CDS
TTTAGAGCCGGGGCATGAAG+TGG 0.598649 8.1:+44853923 None:intergenic
TATGTGAATATAGTTCAGAT+GGG 0.599955 8.1:+44853565 None:intergenic
TTCACATAGAAGGATATGAA+GGG 0.600604 8.1:-44853550 None:intergenic
AATGCTCAATCTGTCGGTGT+CGG 0.603239 8.1:+44855376 None:intergenic
GCAATTCAAGATAGAAGGAG+GGG 0.610013 8.1:+44854983 None:intergenic
CGGTAGAATGCTCAATCTGT+CGG 0.610786 8.1:+44855370 None:intergenic
GTGCAATTCAAGATAGAAGG+AGG 0.611026 8.1:+44854981 None:intergenic
CAGGTATCTTCATCTCTGGA+AGG 0.611083 8.1:-44855273 MS.gene34740:CDS
TTGAACCTCTTGGAGGAAGG+TGG 0.615042 8.1:+44854248 None:intergenic
TCTTATAATTCAAAGCAACA+AGG 0.615876 8.1:-44853798 MS.gene34740:intron
TTGTTGGTGATATCTGGATG+AGG 0.618069 8.1:-44854421 MS.gene34740:CDS
TGTTGGTGATATCTGGATGA+GGG 0.623457 8.1:-44854420 MS.gene34740:CDS
GGTGGGGCCCAATGGCAGCA+CGG 0.623491 8.1:+44855063 None:intergenic
TGCAATTCAAGATAGAAGGA+GGG 0.628277 8.1:+44854982 None:intergenic
TTGTACACGATAACAAGTGA+TGG 0.628832 8.1:+44853953 None:intergenic
TTTGCACCATTCGACATCAG+AGG 0.629433 8.1:-44854030 MS.gene34740:intron
ACTTCACCTCTGATGTCGAA+TGG 0.635361 8.1:+44854024 None:intergenic
GATGGAACTTTACAATGTCG+CGG 0.638613 8.1:+44855144 None:intergenic
CAACGAATACATAGATAAGT+GGG 0.642156 8.1:-44855087 MS.gene34740:CDS
TTGTTATCGTGTACAAGCAG+TGG 0.643881 8.1:-44853946 MS.gene34740:CDS
CAATTCAAGATAGAAGGAGG+GGG 0.645221 8.1:+44854984 None:intergenic
AACAGCCTGAAATATTCAGA+AGG 0.650806 8.1:-44855208 MS.gene34740:CDS
CAATGCCACCTTCCTCCAAG+AGG 0.652253 8.1:-44854253 MS.gene34740:CDS
CCTACCAACTAAAACTTCCG+TGG 0.656922 8.1:-44855318 MS.gene34740:CDS
AAAGGAAACAATTGAACACA+CGG 0.657903 8.1:+44854343 None:intergenic
GAAGATACCTGGGAGAGACG+AGG 0.670890 8.1:+44855285 None:intergenic
GATCCAAAGTTTCAAGCATG+GGG 0.677841 8.1:+44854309 None:intergenic
GGGAGAGACGAGGCTCATGG+TGG 0.702189 8.1:+44855295 None:intergenic
ACAGGTATTCGACTTCTCCG+AGG 0.722887 8.1:-44855246 MS.gene34740:CDS
ATTGTAAAGTTCCATCTCAG+CGG 0.731155 8.1:-44855137 MS.gene34740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAATAAATAAATAAATA+AGG + chr8.1:44854059-44854078 None:intergenic 0.0%
!!! TTTTTTTTTTTTTTAAGGAA+AGG - chr8.1:44854422-44854441 MS.gene34740:CDS 10.0%
!! AATTTAATTGAATCAATCTT+TGG - chr8.1:44855227-44855246 MS.gene34740:CDS 15.0%
!! TTTAACAAAACAAAACATAT+AGG + chr8.1:44854293-44854312 None:intergenic 15.0%
!!! GTTTTGTTAAAGAAACTATT+AGG - chr8.1:44854302-44854321 MS.gene34740:CDS 20.0%
! AACATTGAGAAAAAAACGTT+GGG - chr8.1:44854812-44854831 MS.gene34740:intron 25.0%
! AAGAAACTATTAGGAAAAAC+TGG - chr8.1:44854311-44854330 MS.gene34740:CDS 25.0%
! ACTCTAAATATAGTTCTTGA+AGG + chr8.1:44853949-44853968 None:intergenic 25.0%
! AGAAACTATTAGGAAAAACT+GGG - chr8.1:44854312-44854331 MS.gene34740:CDS 25.0%
! TAAATATAGTTCTTGAAGGT+GGG + chr8.1:44853945-44853964 None:intergenic 25.0%
! TAACATTGAGAAAAAAACGT+TGG - chr8.1:44854811-44854830 MS.gene34740:intron 25.0%
! TATGTGAATATAGTTCAGAT+GGG + chr8.1:44855426-44855445 None:intergenic 25.0%
! TCAATTGTTTCCTTTCTATT+TGG - chr8.1:44854653-44854672 MS.gene34740:intron 25.0%
! TCTTATAATTCAAAGCAACA+AGG - chr8.1:44855190-44855209 MS.gene34740:CDS 25.0%
! TTATAAGAACTTCAAGCATA+TGG + chr8.1:44855178-44855197 None:intergenic 25.0%
! TTCTTTGTAAGTAATTAGCA+AGG - chr8.1:44854151-44854170 MS.gene34740:CDS 25.0%
!! TATTTAGAGTTTGATTCTGA+TGG - chr8.1:44853959-44853978 MS.gene34740:CDS 25.0%
!!! TTATGTAAGTTCTGAAATTC+TGG + chr8.1:44855095-44855114 None:intergenic 25.0%
AAAAAAACGTTGGGCATAAT+CGG - chr8.1:44854821-44854840 MS.gene34740:intron 30.0%
AAAGGAAACAATTGAACACA+CGG + chr8.1:44854648-44854667 None:intergenic 30.0%
AAATATAGTTCTTGAAGGTG+GGG + chr8.1:44853944-44853963 None:intergenic 30.0%
AGTTTCAACATCTTCAACAA+TGG + chr8.1:44854614-44854633 None:intergenic 30.0%
ATTCACATAGAAGGATATGA+AGG - chr8.1:44855437-44855456 MS.gene34740:CDS 30.0%
CAACGAATACATAGATAAGT+GGG - chr8.1:44853901-44853920 MS.gene34740:CDS 30.0%
CATGCATTTGTTTGAGTATA+GGG + chr8.1:44854913-44854932 None:intergenic 30.0%
CTAAATATAGTTCTTGAAGG+TGG + chr8.1:44853946-44853965 None:intergenic 30.0%
CTATGTGAATATAGTTCAGA+TGG + chr8.1:44855427-44855446 None:intergenic 30.0%
CTGAACTATATTCACATAGA+AGG - chr8.1:44855428-44855447 MS.gene34740:CDS 30.0%
GAGGTGAAGTTCTATTATTA+TGG - chr8.1:44854977-44854996 MS.gene34740:CDS 30.0%
TAAAACACACATTTGAGTCA+TGG - chr8.1:44855314-44855333 MS.gene34740:CDS 30.0%
TCATGCATTTGTTTGAGTAT+AGG + chr8.1:44854914-44854933 None:intergenic 30.0%
TTAGACGTGCAAATGAAAAA+TGG - chr8.1:44854184-44854203 MS.gene34740:CDS 30.0%
! ATCATCACCTTTAAGAACAT+GGG - chr8.1:44855364-44855383 MS.gene34740:CDS 30.0%
!! ATCTTTGGTCTTTTTATGTG+TGG - chr8.1:44855242-44855261 MS.gene34740:CDS 30.0%
AAAGTCTTGAAACGGAACTT+TGG + chr8.1:44854240-44854259 None:intergenic 35.0%
AACAGCCTGAAATATTCAGA+AGG - chr8.1:44853780-44853799 MS.gene34740:intron 35.0%
AGAAGATTAGAGTTGAAGCA+TGG + chr8.1:44855134-44855153 None:intergenic 35.0%
ATGATCCAAAGTTTCAAGCA+TGG + chr8.1:44854684-44854703 None:intergenic 35.0%
ATGCTTCAACTCTAATCTTC+TGG - chr8.1:44855133-44855152 MS.gene34740:CDS 35.0%
ATTGTAAAGTTCCATCTCAG+CGG - chr8.1:44853851-44853870 MS.gene34740:CDS 35.0%
CATGTTCTTAAAGGTGATGA+TGG + chr8.1:44855365-44855384 None:intergenic 35.0%
CGTGCTTTGTATCTATTTGA+AGG + chr8.1:44854787-44854806 None:intergenic 35.0%
CTTTGTATCTATTTGAAGGC+AGG + chr8.1:44854783-44854802 None:intergenic 35.0%
GAAGATTAGAGTTGAAGCAT+GGG + chr8.1:44855133-44855152 None:intergenic 35.0%
GCAACGAATACATAGATAAG+TGG - chr8.1:44853900-44853919 MS.gene34740:CDS 35.0%
GTCATAACGTTCATAAACCA+TGG + chr8.1:44854354-44854373 None:intergenic 35.0%
TCGCCTAAAAAGTCTTGAAA+CGG + chr8.1:44854248-44854267 None:intergenic 35.0%
TGATCCAAAGTTTCAAGCAT+GGG + chr8.1:44854683-44854702 None:intergenic 35.0%
TGCAATTCAAGATAGAAGGA+GGG + chr8.1:44854009-44854028 None:intergenic 35.0%
TGCAGTGTATTCAAAAAGAC+AGG + chr8.1:44854544-44854563 None:intergenic 35.0%
TTGGTGCAATTCAAGATAGA+AGG + chr8.1:44854013-44854032 None:intergenic 35.0%
TTGTACACGATAACAAGTGA+TGG + chr8.1:44855038-44855057 None:intergenic 35.0%
! AATTCTGGATGATTTAGAGC+CGG + chr8.1:44855080-44855099 None:intergenic 35.0%
! CATCATCACCTTTAAGAACA+TGG - chr8.1:44855363-44855382 MS.gene34740:CDS 35.0%
! GAGTTTAATGAGTTGAACCA+TGG - chr8.1:44854334-44854353 MS.gene34740:CDS 35.0%
! GTTCCGTTTCAAGACTTTTT+AGG - chr8.1:44854242-44854261 MS.gene34740:CDS 35.0%
! TGGTCTTTTTATGTGTGGAT+TGG - chr8.1:44855247-44855266 MS.gene34740:CDS 35.0%
AACAAGTGATGGCTTAGTAC+AGG + chr8.1:44855027-44855046 None:intergenic 40.0%
AGATGGGATTCGAGACATTT+AGG + chr8.1:44855410-44855429 None:intergenic 40.0%
ATCTAGATAACCGCAGTTAG+TGG - chr8.1:44854382-44854401 MS.gene34740:CDS 40.0%
ATGGAACTTTACAATGTCGC+GGG + chr8.1:44853846-44853865 None:intergenic 40.0%
CAATTCAAGATAGAAGGAGG+GGG + chr8.1:44854007-44854026 None:intergenic 40.0%
CCAGAAGAAGAAGATGATGA+TGG - chr8.1:44853581-44853600 MS.gene34740:CDS 40.0%
CCATCATCATCTTCTTCTTC+TGG + chr8.1:44853584-44853603 None:intergenic 40.0%
CTACCTGAGAGAAACAAGAT+TGG + chr8.1:44854032-44854051 None:intergenic 40.0%
GAAATTCCCATTGAACCTCT+TGG + chr8.1:44854753-44854772 None:intergenic 40.0%
GAGTTTGATTCTGATGGAAC+TGG - chr8.1:44853965-44853984 MS.gene34740:CDS 40.0%
GATCCAAAGTTTCAAGCATG+GGG + chr8.1:44854682-44854701 None:intergenic 40.0%
GATGGAACTTTACAATGTCG+CGG + chr8.1:44853847-44853866 None:intergenic 40.0%
GCAATTCAAGATAGAAGGAG+GGG + chr8.1:44854008-44854027 None:intergenic 40.0%
GCGTACCTTCTGAATATTTC+AGG + chr8.1:44853788-44853807 None:intergenic 40.0%
GGTTCAATGGGAATTTCTCT+TGG - chr8.1:44854756-44854775 MS.gene34740:intron 40.0%
GTGCAATTCAAGATAGAAGG+AGG + chr8.1:44854010-44854029 None:intergenic 40.0%
TCTAGATAACCGCAGTTAGT+GGG - chr8.1:44854383-44854402 MS.gene34740:CDS 40.0%
TTACAATGTCGCGGGAATTA+CGG + chr8.1:44853838-44853857 None:intergenic 40.0%
TTCCAGAGATGAAGATACCT+GGG + chr8.1:44853716-44853735 None:intergenic 40.0%
TTGGTAGGAAGGACATGATA+TGG + chr8.1:44853658-44853677 None:intergenic 40.0%
TTGTTATCGTGTACAAGCAG+TGG - chr8.1:44855042-44855061 MS.gene34740:CDS 40.0%
! ATTCTGGATGATTTAGAGCC+GGG + chr8.1:44855079-44855098 None:intergenic 40.0%
! ATTGCTTTGAGTTCAGTCCT+AGG - chr8.1:44854702-44854721 MS.gene34740:intron 40.0%
! CAATCTTGTTTCTCTCAGGT+AGG - chr8.1:44854030-44854049 MS.gene34740:intron 40.0%
! CAGAAGGTACGCTTTATATG+TGG - chr8.1:44853796-44853815 MS.gene34740:intron 40.0%
! GGTGTTATCTCGTATGATCA+TGG - chr8.1:44853872-44853891 MS.gene34740:CDS 40.0%
! TGAATACACTGCAGTCTTGT+TGG - chr8.1:44854551-44854570 MS.gene34740:intron 40.0%
!! TGTTGGTGATATCTGGATGA+GGG - chr8.1:44854568-44854587 MS.gene34740:intron 40.0%
!! TTGTTGGTGATATCTGGATG+AGG - chr8.1:44854567-44854586 MS.gene34740:intron 40.0%
!!! GGAAGTTTTAGTTGGTAGGA+AGG + chr8.1:44853669-44853688 None:intergenic 40.0%
AATGCTCAATCTGTCGGTGT+CGG + chr8.1:44853615-44853634 None:intergenic 45.0%
ACTTCACCTCTGATGTCGAA+TGG + chr8.1:44854967-44854986 None:intergenic 45.0%
ATGCCCCATGCTTGAAACTT+TGG - chr8.1:44854676-44854695 MS.gene34740:intron 45.0%
ATTCCCATTGAACCTCTTGG+AGG + chr8.1:44854750-44854769 None:intergenic 45.0%
CAGGTATCTTCATCTCTGGA+AGG - chr8.1:44853715-44853734 MS.gene34740:intron 45.0%
CCTACCAACTAAAACTTCCG+TGG - chr8.1:44853670-44853689 MS.gene34740:intron 45.0%
CGACAGATTGAGCATTCTAC+CGG - chr8.1:44853619-44853638 MS.gene34740:CDS 45.0%
CGGTAGAATGCTCAATCTGT+CGG + chr8.1:44853621-44853640 None:intergenic 45.0%
CTTCCAGAGATGAAGATACC+TGG + chr8.1:44853717-44853736 None:intergenic 45.0%
CTTCCTCCAAGAGGTTCAAT+GGG - chr8.1:44854744-44854763 MS.gene34740:intron 45.0%
GTTCCTCCTACAAGCAACAA+AGG + chr8.1:44855343-44855362 None:intergenic 45.0%
TAACCGCAGTTAGTGGGATA+AGG - chr8.1:44854389-44854408 MS.gene34740:CDS 45.0%
TGCAGGTCCCATGTTCTTAA+AGG + chr8.1:44855374-44855393 None:intergenic 45.0%
TTTGCACCATTCGACATCAG+AGG - chr8.1:44854958-44854977 MS.gene34740:intron 45.0%
! CATGGGGCATCCAAATAGAA+AGG + chr8.1:44854666-44854685 None:intergenic 45.0%
! GCACCAATCTTGTTTCTCTC+AGG - chr8.1:44854026-44854045 MS.gene34740:intron 45.0%
! TAGGAACTGTTGTTGACTGC+AGG + chr8.1:44855391-44855410 None:intergenic 45.0%
! TCATCTCTGGAAGGATCTAC+AGG - chr8.1:44853724-44853743 MS.gene34740:intron 45.0%
! TGGTGTCCTTTGTTGCTTGT+AGG - chr8.1:44855334-44855353 MS.gene34740:CDS 45.0%
! TGTCCTTTGTTGCTTGTAGG+AGG - chr8.1:44855337-44855356 MS.gene34740:CDS 45.0%
! TTCTGGATGATTTAGAGCCG+GGG + chr8.1:44855078-44855097 None:intergenic 45.0%
!! GCAGTCTTGTTGGTGATATC+TGG - chr8.1:44854561-44854580 MS.gene34740:intron 45.0%
!! TAGCCTTATCCCACTAACTG+CGG + chr8.1:44854395-44854414 None:intergenic 45.0%
!!! CCACGGAAGTTTTAGTTGGT+AGG + chr8.1:44853673-44853692 None:intergenic 45.0%
!!! TTGATTTTTTTTTTTTTTTA+AGG - chr8.1:44854417-44854436 MS.gene34740:CDS 5.0%
ACAGGTATTCGACTTCTCCG+AGG - chr8.1:44853742-44853761 MS.gene34740:intron 50.0%
ACGAGATAACACCGCTGAGA+TGG + chr8.1:44853865-44853884 None:intergenic 50.0%
ATATGGCACAGGAGTGAGTC+CGG + chr8.1:44853641-44853660 None:intergenic 50.0%
CCATTGAACCTCTTGGAGGA+AGG + chr8.1:44854746-44854765 None:intergenic 50.0%
CCTTCCTCCAAGAGGTTCAA+TGG - chr8.1:44854743-44854762 MS.gene34740:intron 50.0%
CTCCCAGGTATCTTCATCTC+TGG - chr8.1:44853711-44853730 MS.gene34740:intron 50.0%
GGAAGGACATGATATGGCAC+AGG + chr8.1:44853652-44853671 None:intergenic 50.0%
GTTGGGCATAATCGGCAACT+TGG - chr8.1:44854829-44854848 MS.gene34740:intron 50.0%
TTCGTCGTCGTAAGTGTCCT+CGG + chr8.1:44853762-44853781 None:intergenic 50.0%
TTGAACCTCTTGGAGGAAGG+TGG + chr8.1:44854743-44854762 None:intergenic 50.0%
!! GGCATTGGTACTTCGTTCCT+AGG + chr8.1:44854722-44854741 None:intergenic 50.0%
!! GTGGCCACGGAAGTTTTAGT+TGG + chr8.1:44853677-44853696 None:intergenic 50.0%
CAATGCCACCTTCCTCCAAG+AGG - chr8.1:44854735-44854754 MS.gene34740:intron 55.0%
CTCTTGGAGGAAGGTGGCAT+TGG + chr8.1:44854737-44854756 None:intergenic 55.0%
GAAGATACCTGGGAGAGACG+AGG + chr8.1:44853706-44853725 None:intergenic 55.0%
TTCTTGAAGGTGGGGCCCAA+TGG + chr8.1:44853936-44853955 None:intergenic 55.0%
! CGGCAACTTGGAGAGTGTGA+TGG - chr8.1:44854841-44854860 MS.gene34740:intron 55.0%
! TTTAGAGCCGGGGCATGAAG+TGG + chr8.1:44855068-44855087 None:intergenic 55.0%
AAGTGGGTCCGTGCTGCCAT+TGG - chr8.1:44853917-44853936 MS.gene34740:CDS 60.0%
AGTGGGTCCGTGCTGCCATT+GGG - chr8.1:44853918-44853937 MS.gene34740:CDS 60.0%
CCATGAGCCTCGTCTCTCCC+AGG - chr8.1:44853696-44853715 MS.gene34740:intron 65.0%
CCTGGGAGAGACGAGGCTCA+TGG + chr8.1:44853699-44853718 None:intergenic 65.0%
GACGAGGCTCATGGTGGCCA+CGG + chr8.1:44853690-44853709 None:intergenic 65.0%
GCAGTGGCCACTTCATGCCC+CGG - chr8.1:44855058-44855077 MS.gene34740:CDS 65.0%
GGGAGAGACGAGGCTCATGG+TGG + chr8.1:44853696-44853715 None:intergenic 65.0%
GGTGGGGCCCAATGGCAGCA+CGG + chr8.1:44853928-44853947 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 44853551 44855459 44853551 ID=MS.gene34740
chr8.1 mRNA 44853551 44855459 44853551 ID=MS.gene34740.t1;Parent=MS.gene34740
chr8.1 exon 44854963 44855459 44854963 ID=MS.gene34740.t1.exon1;Parent=MS.gene34740.t1
chr8.1 CDS 44854963 44855459 44854963 ID=cds.MS.gene34740.t1;Parent=MS.gene34740.t1
chr8.1 exon 44854031 44854445 44854031 ID=MS.gene34740.t1.exon2;Parent=MS.gene34740.t1
chr8.1 CDS 44854031 44854445 44854031 ID=cds.MS.gene34740.t1;Parent=MS.gene34740.t1
chr8.1 exon 44853799 44853987 44853799 ID=MS.gene34740.t1.exon3;Parent=MS.gene34740.t1
chr8.1 CDS 44853799 44853987 44853799 ID=cds.MS.gene34740.t1;Parent=MS.gene34740.t1
chr8.1 exon 44853551 44853654 44853551 ID=MS.gene34740.t1.exon4;Parent=MS.gene34740.t1
chr8.1 CDS 44853551 44853654 44853551 ID=cds.MS.gene34740.t1;Parent=MS.gene34740.t1
Gene Sequence

>MS.gene34740

ATGACGGAGTCGAGCATCAAACTTCAGAAACCAGAAGAAGAAGATGATGATGGTGAAGTCACCGACACCGACAGATTGAGCATTCTACCGGACTCACTCCTGTGCCATATCATGTCCTTCCTACCAACTAAAACTTCCGTGGCCACCATGAGCCTCGTCTCTCCCAGGTATCTTCATCTCTGGAAGGATCTACAGGTATTCGACTTCTCCGAGGACACTTACGACGACGAACAGCCTGAAATATTCAGAAGGTACGCTTTATATGTGGACAATGTGCTTGCTCTCCGTAATTCCCGCGACATTGTAAAGTTCCATCTCAGCGGTGTTATCTCGTATGATCATGGAAGTCGCAACGAATACATAGATAAGTGGGTCCGTGCTGCCATTGGGCCCCACCTTCAAGAACTATATTTAGAGTTTGATTCTGATGGAACTGGAGAGACTGTCATGATTCCCCCTCCTTCTATCTTGAATTGCACCAATCTTGTTTCTCTCAGGTAGGTAACCTTATTTATTTATTTATTTTTATTTTGAATTGAAAAATTTATTAAAAGAGTAAAATGTAACGAAAACATAAGTGTATTATTTAGTTCACTTCAGTTCTTTGTAAGTAATTAGCAAGGATTTCTTGTTTTAGACGTGCAAATGAAAAATGGTAATATGCTAAAAAATCAATAATATATACACCAAAGTTCCGTTTCAAGACTTTTTAGGCGATGTGTCAAAGCTATAGATTAGACCTATATGTTTTGTTTTGTTAAAGAAACTATTAGGAAAAACTGGGAGTTTAATGAGTTGAACCATGGTTTATGAACGTTATGACATATTTTGATCTAGATAACCGCAGTTAGTGGGATAAGGCTAAATTGATTTTTTTTTTTTTTTAAGGAAAGGCTAGATTGTTGTCGTCGAATCTCACTTGCATGATTTCATCTCATGTTGTCTTTCTATTTTTAATTCATAAAGTAGTAGATATAGAATGTTATGTAACCTGTCTTTTTGAATACACTGCAGTCTTGTTGGTGATATCTGGATGAGGGATCAACATTCATTTCATTTTCCATTGTTGAAGATGTTGAAACTCGACATAGACACCGTGTGTTCAATTGTTTCCTTTCTATTTGGATGCCCCATGCTTGAAACTTTGGATCATTGCTTTGAGTTCAGTCCTAGGAACGAAGTACCAATGCCACCTTCCTCCAAGAGGTTCAATGGGAATTTCTCTTGGACCTGCCTTCAAATAGATACAAAGCACGATAGTAACATTGAGAAAAAAACGTTGGGCATAATCGGCAACTTGGAGAGTGTGATGGAAGCATATCTTGATGTCTTTTCTCTGTGTGAAAGTGAATTTGTTTACCCTATACTCAAACAAATGCATGATCAATTGCATTTTTCACATCTACTTTTGCACCATTCGACATCAGAGGTGAAGTTCTATTATTATGGTTTTTATGTATTTGTATAGAAAGTCCTGTACTAAGCCATCACTTGTTATCGTGTACAAGCAGTGGCCACTTCATGCCCCGGCTCTAAATCATCCAGAATTTCAGAACTTACATAATCTAAAGTTCAAACTCCCATGCTTCAACTCTAATCTTCTGGTCAACGTGATTGAGAAATGCCATATGCTTGAAGTTCTTATAATTCAAAGCAACAAGGTTTGTTGACTTCTCAATTTAATTGAATCAATCTTTGGTCTTTTTATGTGTGGATTGGATGTATGAATTAATCTATACACATATATGATATTTTTTTATATATAAAACACACATTTGAGTCATGGTGTCCTTTGTTGCTTGTAGGAGGAACCATCATCACCTTTAAGAACATGGGACCTGCAGTCAACAACAGTTCCTAAATGTCTCGAATCCCATCTGAACTATATTCACATAGAAGGATATGAAGG
Protein sequence

>MS.gene34740.t1

MTESSIKLQKPEEEDDDGEVTDTDRLSILPDSLLCHIMSFLPTKTSVATMSLVSPRYLHLWKDLQVFDFSEDTYDDEQPEIFRRYALYVDNVLALRNSRDIVKFHLSGVISYDHGSRNEYIDKWVRAAIGPHLQELYLEFDSDGTGETVMIPPPSILNCTNLVSLSLVGDIWMRDQHSFHFPLLKMLKLDIDTVCSIVSFLFGCPMLETLDHCFEFSPRNEVPMPPSSKRFNGNFSWTCLQIDTKHDSNIEKKTLGIIGNLESVMEAYLDVFSLCESEFVYPILKQMHDQLHFSHLLLHHSTSESCTKPSLVIVYKQWPLHAPALNHPEFQNLHNLKFKLPCFNSNLLVNVIEKCHMLEVLIIQSNKEEPSSPLRTWDLQSTTVPKCLESHLNYIHIEGYE