AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34706

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34706.t1 MTR_8g070950 64.938 405 125 9 1 394 41 439 2.40e-165 471
MS.gene34706.t1 MTR_8g062010 56.824 403 161 7 1 393 35 434 8.41e-134 390
MS.gene34706.t1 MTR_8g062310 55.584 394 158 10 4 394 54 433 6.30e-133 388
MS.gene34706.t1 MTR_8g062300 53.118 433 160 10 1 393 52 481 4.93e-131 385
MS.gene34706.t1 MTR_8g469020 51.740 431 162 13 2 394 34 456 1.10e-128 378
MS.gene34706.t1 MTR_8g062080 53.511 413 160 14 1 393 49 449 1.56e-127 375
MS.gene34706.t1 MTR_8g058965 51.860 430 160 12 2 393 40 460 7.77e-121 358
MS.gene34706.t1 MTR_8g467790 54.945 364 110 12 1 343 174 504 1.99e-108 328
MS.gene34706.t1 MTR_4g050250 47.781 383 143 14 1 335 47 420 6.30e-94 288
MS.gene34706.t1 MTR_2g086020 73.973 146 37 1 250 394 175 320 2.43e-66 214
MS.gene34706.t1 MTR_2g086020 44.604 139 67 5 1 131 1 137 6.02e-22 95.9
MS.gene34706.t1 MTR_2g083840 39.089 417 216 14 3 389 49 457 7.63e-62 206
MS.gene34706.t1 MTR_8g014260 37.981 416 220 14 4 389 44 451 6.08e-59 198
MS.gene34706.t1 MTR_8g062270 35.613 424 226 12 1 388 41 453 1.36e-56 192
MS.gene34706.t1 MTR_8g468810 38.318 428 210 21 1 389 51 463 1.38e-55 190
MS.gene34706.t1 MTR_8g467800 37.116 423 226 16 2 394 46 458 1.66e-55 189
MS.gene34706.t1 MTR_5g092960 34.884 430 223 18 3 389 47 462 3.15e-52 181
MS.gene34706.t1 MTR_8g468840 37.018 389 203 14 2 356 40 420 2.46e-50 176
MS.gene34706.t1 MTR_8g058970 34.831 445 222 20 1 389 45 477 9.09e-50 174
MS.gene34706.t1 MTR_4g032730 34.332 434 229 15 3 389 43 467 6.12e-48 169
MS.gene34706.t1 MTR_8g468760 35.142 424 200 14 1 388 34 418 6.61e-48 168
MS.gene34706.t1 MTR_8g445780 36.438 365 191 16 4 342 41 390 2.30e-47 166
MS.gene34706.t1 MTR_8g468810 37.612 335 160 18 1 300 51 371 8.13e-36 135
MS.gene34706.t1 MTR_8g014160 31.235 413 205 16 2 389 67 425 1.51e-35 135
MS.gene34706.t1 MTR_5g092960 34.524 336 171 14 3 300 47 371 2.26e-34 131
MS.gene34706.t1 MTR_4g024710 36.054 294 165 11 111 389 62 347 4.51e-34 129
MS.gene34706.t1 MTR_8g077460 45.455 143 75 3 248 389 235 375 4.18e-30 119
MS.gene34706.t1 MTR_8g468810 39.535 258 115 15 1 230 51 295 1.41e-29 117
MS.gene34706.t1 MTR_8g468810 39.535 258 115 15 1 230 51 295 2.69e-29 116
MS.gene34706.t1 MTR_8g468810 39.535 258 115 15 1 230 51 295 4.18e-29 116
MS.gene34706.t1 MTR_4g024745 35.036 274 155 11 109 367 14 279 3.14e-26 107
MS.gene34706.t1 MTR_8g014210 36.134 238 132 10 165 389 1 231 6.59e-26 104
MS.gene34706.t1 MTR_8g467800 36.863 255 132 12 2 234 46 293 5.85e-25 104
MS.gene34706.t1 MTR_3g099800 63.158 76 26 2 302 375 19 94 1.29e-23 94.7
MS.gene34706.t1 MTR_5g084070 25.570 395 261 12 2 376 39 420 2.12e-15 77.8
MS.gene34706.t1 MTR_5g080710 25.714 385 243 12 2 361 59 425 1.50e-14 75.5
MS.gene34706.t1 MTR_8g014230 43.590 78 43 1 300 377 17 93 3.21e-14 68.6
MS.gene34706.t1 MTR_5g021550 43.590 117 50 7 4 105 47 162 7.35e-14 69.3
MS.gene34706.t1 MTR_7g098510 24.816 407 228 17 2 389 44 391 3.28e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34706.t1 AT5G50270 27.861 402 237 15 3 374 30 408 4.66e-19 88.6
MS.gene34706.t1 AT5G56560 26.634 413 250 17 3 389 33 418 6.91e-17 82.4
MS.gene34706.t1 AT5G56560 28.475 295 182 10 111 389 326 607 1.45e-13 72.8
MS.gene34706.t1 AT2G26030 26.835 436 249 21 2 390 30 442 2.38e-13 71.6
MS.gene34706.t1 AT2G26030 26.835 436 249 21 2 390 30 442 2.38e-13 71.6
MS.gene34706.t1 AT5G50270 27.309 249 150 9 3 229 30 269 1.72e-12 68.2
MS.gene34706.t1 AT4G10400 26.119 402 263 15 2 389 28 409 2.54e-12 68.6
MS.gene34706.t1 AT4G10400 26.119 402 263 15 2 389 28 409 2.54e-12 68.6
MS.gene34706.t1 AT4G10400 26.119 402 263 15 2 389 28 409 2.54e-12 68.6
MS.gene34706.t1 AT5G38570 24.372 398 240 12 3 389 29 376 1.17e-11 66.2
MS.gene34706.t1 AT4G13965 25.547 411 254 16 2 386 36 420 8.65e-11 63.5

Find 85 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGGAAACTTTAATAATA+TGG 0.213057 8.1:-45636859 MS.gene34706:CDS
CACTTTCTGCCCCTGTTCTA+AGG 0.248751 8.1:-45636407 MS.gene34706:CDS
TTTGCGGAACGGGCTTGTTT+TGG 0.301181 8.1:-45636012 MS.gene34706:CDS
AGCACAGTGTTGACGAATAA+TGG 0.325293 8.1:+45637757 None:intergenic
CAGTTGATGAAGAGGGTAGA+AGG 0.327420 8.1:+45637580 None:intergenic
GGGAAAACATGTTTAAGTTA+TGG 0.346779 8.1:-45637669 MS.gene34706:CDS
CTCGTAGGTGGCGCCACCTC+TGG 0.349675 8.1:-45637840 MS.gene34706:CDS
CGTGTCACATTGGGCATCAT+TGG 0.351168 8.1:-45636876 MS.gene34706:CDS
ATTGGTAGATTTCAATCTCT+TGG 0.357522 8.1:+45636941 None:intergenic
ATGTTTCAACTCAAATCTTC+TGG 0.358687 8.1:-45636340 MS.gene34706:CDS
AACCATGTTATATCTCCTTA+AGG 0.368169 8.1:-45635879 None:intergenic
AACTCTGGATACCTTAGAAC+AGG 0.387304 8.1:+45636396 None:intergenic
CTCGGATGTCTTTGAGAATA+GGG 0.388547 8.1:+45636787 None:intergenic
TTATGGATCCGTACTGCTAC+TGG 0.402373 8.1:-45637652 MS.gene34706:CDS
TCTCGGATGTCTTTGAGAAT+AGG 0.404973 8.1:+45636786 None:intergenic
TCTCTCCGTCTGCCCCGTGC+TGG 0.428073 8.1:-45637039 MS.gene34706:CDS
GAGCTGGAGGAAGGTGGAAC+AGG 0.435822 8.1:+45636963 None:intergenic
CGATCTAATTTGCGAAACTC+TGG 0.442892 8.1:+45636381 None:intergenic
ACGGAGAGAAAGGAAACTAT+TGG 0.444858 8.1:+45637053 None:intergenic
AGATTATCTGAGATAACAAT+TGG 0.445912 8.1:-45635927 MS.gene34706:CDS
ATAGATCCCCGTGTCACATT+GGG 0.455209 8.1:-45636885 MS.gene34706:CDS
CACGGGGCAGACGGAGAGAA+AGG 0.456042 8.1:+45637043 None:intergenic
AAAGTAGAGAAATATCATAT+TGG 0.462773 8.1:+45636760 None:intergenic
CTACCAATCACAATTTCACT+TGG 0.468189 8.1:-45636926 MS.gene34706:CDS
CTGCCCCGTGCTGGAAACAC+TGG 0.475059 8.1:-45637030 MS.gene34706:CDS
AGATTTCAATCTCTTGGAGC+TGG 0.476427 8.1:+45636947 None:intergenic
AGTTGATGAAGAGGGTAGAA+GGG 0.486405 8.1:+45637581 None:intergenic
CTTGTACGTGAATATATGTC+AGG 0.499325 8.1:+45636078 None:intergenic
AGTCCAAGTGAAATTGTGAT+TGG 0.501196 8.1:+45636923 None:intergenic
CAATCCAGTGTTTCCAGCAC+GGG 0.503073 8.1:+45637026 None:intergenic
GAGATAGAGTTCTTCAAGGT+GGG 0.506876 8.1:+45637627 None:intergenic
TTTCTCGATGTCCTTTCCCC+AGG 0.512089 8.1:-45636828 MS.gene34706:CDS
CACCTTAAGGAGATATAACA+TGG 0.521086 8.1:+45635877 None:intergenic
CTTGTAGCTGCTGCCAGAGG+TGG 0.531131 8.1:+45637827 None:intergenic
AATCTCTTGGAGCTGGAGGA+AGG 0.531230 8.1:+45636954 None:intergenic
TGTTTCCAGCACGGGGCAGA+CGG 0.535232 8.1:+45637034 None:intergenic
TATAGATCCCCGTGTCACAT+TGG 0.538369 8.1:-45636886 MS.gene34706:CDS
GAATGTTGGGGAATCTGCGG+AGG 0.539616 8.1:+45637731 None:intergenic
AAATATCATATTGGTCATCT+CGG 0.543175 8.1:+45636769 None:intergenic
TGGAGAGATAGAGTTCTTCA+AGG 0.545549 8.1:+45637623 None:intergenic
AATGATGCCCAATGTGACAC+GGG 0.545808 8.1:+45636878 None:intergenic
CTTAGAACAGGGGCAGAAAG+TGG 0.548297 8.1:+45636408 None:intergenic
TTCGCGTCATTTGACATCAA+AGG 0.548380 8.1:-45636739 MS.gene34706:intron
ATTCACGTACAAGGATATCA+AGG 0.550700 8.1:-45636068 MS.gene34706:CDS
ATTCGCCTTCACCTGGGGAA+AGG 0.554606 8.1:+45636817 None:intergenic
AGATACAGCTTCTTCAGTGA+TGG 0.555293 8.1:+45637098 None:intergenic
TTTCAATCTCTTGGAGCTGG+AGG 0.561546 8.1:+45636950 None:intergenic
GCAACAACCTCGTTTCCCTC+AGG 0.563749 8.1:-45637558 MS.gene34706:intron
CAATGATGCCCAATGTGACA+CGG 0.564076 8.1:+45636877 None:intergenic
CTATGAGTCTTGTGTCTCGT+AGG 0.564360 8.1:-45637855 MS.gene34706:CDS
GATGTCCTTTCCCCAGGTGA+AGG 0.565441 8.1:-45636822 MS.gene34706:CDS
CTGACATATATTCACGTACA+AGG 0.572025 8.1:-45636077 MS.gene34706:CDS
TTCGAATGTTGGGGAATCTG+CGG 0.575364 8.1:+45637728 None:intergenic
TATGGATCCGTACTGCTACT+GGG 0.576454 8.1:-45637651 MS.gene34706:CDS
ACAATCCAGTGTTTCCAGCA+CGG 0.581383 8.1:+45637025 None:intergenic
GGTTGTTGCAGTTGATGAAG+AGG 0.585577 8.1:+45637572 None:intergenic
TGGAAACTTTAATAATATGG+TGG 0.586549 8.1:-45636856 MS.gene34706:CDS
AGAAGATTTGAGTTGAAACA+TGG 0.586977 8.1:+45636342 None:intergenic
CATTCAGAAACTGTTGACTC+TGG 0.589725 8.1:+45636113 None:intergenic
GGTGGGGCCCAGTAGCAGTA+CGG 0.597691 8.1:+45637644 None:intergenic
AACCACCACCATTGAAAACA+TGG 0.601661 8.1:-45636139 MS.gene34706:CDS
TCTACTCGACTTCGATGCTG+TGG 0.604755 8.1:-45637078 MS.gene34706:CDS
TGAGTCTTGTGTCTCGTAGG+TGG 0.607225 8.1:-45637852 MS.gene34706:CDS
AGATAGAGTTCTTCAAGGTG+GGG 0.613840 8.1:+45637628 None:intergenic
GTTGTTGCAGTTGATGAAGA+GGG 0.613860 8.1:+45637573 None:intergenic
TGTGGCATTTCTCAAGCACA+TGG 0.616958 8.1:+45636316 None:intergenic
CTCTTGGAGCTGGAGGAAGG+TGG 0.621502 8.1:+45636957 None:intergenic
CTTCGTCCTCATCGATAAAG+CGG 0.625742 8.1:+45637800 None:intergenic
GTACGTGAATATATGTCAGG+TGG 0.626657 8.1:+45636081 None:intergenic
TACGTGAATATATGTCAGGT+GGG 0.631686 8.1:+45636082 None:intergenic
GAGTTCAACAGAGTCATGAG+TGG 0.635043 8.1:+45637603 None:intergenic
TAGATTACCTGAGGGAAACG+AGG 0.640585 8.1:+45637551 None:intergenic
GTTAAATTTAAGTTGACACG+TGG 0.645190 8.1:+45635899 None:intergenic
AGACTTGTAGCTGCTGCCAG+AGG 0.652166 8.1:+45637824 None:intergenic
AGAGATAGAGTTCTTCAAGG+TGG 0.653042 8.1:+45637626 None:intergenic
AGTCTTCCGCTTTATCGATG+AGG 0.654779 8.1:-45637806 MS.gene34706:CDS
TGATGAGAACTCGAAGCTTG+TGG 0.658774 8.1:+45636298 None:intergenic
TTCAATGGACAAAACAAACA+AGG 0.664096 8.1:-45635964 MS.gene34706:CDS
GGAAGGTGGAACAGGAACGT+TGG 0.667790 8.1:+45636971 None:intergenic
AATGTTGGGGAATCTGCGGA+GGG 0.668481 8.1:+45637732 None:intergenic
AATCCAGTGTTTCCAGCACG+GGG 0.676114 8.1:+45637027 None:intergenic
TCTCATCATTCAGAGCAGCA+AGG 0.683961 8.1:-45636283 MS.gene34706:intron
ACTCTGGATACCTTAGAACA+GGG 0.693635 8.1:+45636397 None:intergenic
CTCTGGATACCTTAGAACAG+GGG 0.704828 8.1:+45636398 None:intergenic
ATGATGCCCAATGTGACACG+GGG 0.739109 8.1:+45636879 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATATATTTTTGAGTCA+TGG - chr8.1:45637544-45637563 MS.gene34706:intron 15.0%
!!! AGTTTAATTTAATATATGTC+TGG - chr8.1:45637489-45637508 MS.gene34706:intron 15.0%
!!! TATTTTCTTTGATATTTCAA+TGG - chr8.1:45637755-45637774 MS.gene34706:CDS 15.0%
!!! TTAGTTTTCCAAATTTTTAT+CGG - chr8.1:45636350-45636369 MS.gene34706:CDS 15.0%
!! AAAGTAGAGAAATATCATAT+TGG + chr8.1:45636977-45636996 None:intergenic 20.0%
!! AAATTCTGGATAAGTTATAA+CGG + chr8.1:45637155-45637174 None:intergenic 20.0%
!! AACCAATTCTACTTATTTAA+AGG + chr8.1:45636244-45636263 None:intergenic 20.0%
!! AACCTTTAAATAAGTAGAAT+TGG - chr8.1:45636239-45636258 MS.gene34706:intron 20.0%
!! CATTGGAAACTTTAATAATA+TGG - chr8.1:45636875-45636894 MS.gene34706:CDS 20.0%
!!! ATGTTAGTAATTAGTTCTTA+TGG - chr8.1:45637043-45637062 MS.gene34706:CDS 20.0%
!!! CATTTGTTGAATATATTTTG+CGG - chr8.1:45637706-45637725 MS.gene34706:CDS 20.0%
!!! GTGAAGTTTTTATTTTTGAA+TGG - chr8.1:45637017-45637036 MS.gene34706:CDS 20.0%
!!! TCATTGTTTTTATTTTTGAC+TGG - chr8.1:45637201-45637220 MS.gene34706:intron 20.0%
! AAAACAAATTAGATTACCTG+AGG + chr8.1:45636195-45636214 None:intergenic 25.0%
! AAACAAATTAGATTACCTGA+GGG + chr8.1:45636194-45636213 None:intergenic 25.0%
! AAATATCATATTGGTCATCT+CGG + chr8.1:45636968-45636987 None:intergenic 25.0%
! AATTCTGGATAAGTTATAAC+GGG + chr8.1:45637154-45637173 None:intergenic 25.0%
! GTTATAACTTATCCAGAATT+TGG - chr8.1:45637154-45637173 MS.gene34706:intron 25.0%
! TGAAGTAAATTTCCAAATTC+TGG + chr8.1:45637169-45637188 None:intergenic 25.0%
! TGGAAACTTTAATAATATGG+TGG - chr8.1:45636878-45636897 MS.gene34706:CDS 25.0%
!! AGACAAAATTTTCGAATGTT+GGG + chr8.1:45636019-45636038 None:intergenic 25.0%
!! AGATTATCTGAGATAACAAT+TGG - chr8.1:45637807-45637826 MS.gene34706:CDS 25.0%
!! TTAATGCTGGAAATCAATTT+TGG - chr8.1:45636488-45636507 MS.gene34706:intron 25.0%
!!! ATGTTTTTGATATACGTAGT+TGG - chr8.1:45637255-45637274 MS.gene34706:intron 25.0%
ATGTTTCAACTCAAATCTTC+TGG - chr8.1:45637394-45637413 MS.gene34706:intron 30.0%
ATTGGTAGATTTCAATCTCT+TGG + chr8.1:45636796-45636815 None:intergenic 30.0%
CATTGACACCGATAAAAATT+TGG + chr8.1:45636361-45636380 None:intergenic 30.0%
TTCAATGGACAAAACAAACA+AGG - chr8.1:45637770-45637789 MS.gene34706:CDS 30.0%
TTGTTATCATGTACAATCAG+TGG - chr8.1:45637303-45637322 MS.gene34706:intron 30.0%
! GACAAAATTTTCGAATGTTG+GGG + chr8.1:45636018-45636037 None:intergenic 30.0%
! GAGACAAAATTTTCGAATGT+TGG + chr8.1:45636020-45636039 None:intergenic 30.0%
! GGGAAAACATGTTTAAGTTA+TGG - chr8.1:45636065-45636084 MS.gene34706:CDS 30.0%
! GTTGAATATATTTTGCGGAA+CGG - chr8.1:45637711-45637730 MS.gene34706:CDS 30.0%
! TTAAACATGTTTTCCCAATC+CGG + chr8.1:45636061-45636080 None:intergenic 30.0%
! TTGAATATATTTTGCGGAAC+GGG - chr8.1:45637712-45637731 MS.gene34706:CDS 30.0%
!! AGAAGATTTGAGTTGAAACA+TGG + chr8.1:45637395-45637414 None:intergenic 30.0%
!! ATAAGTAGAATTGGTTTGCA+CGG - chr8.1:45636248-45636267 MS.gene34706:intron 30.0%
!! GTTAAATTTAAGTTGACACG+TGG + chr8.1:45637838-45637857 None:intergenic 30.0%
!!! GAGTATTTTGGCAATTTCAT+AGG + chr8.1:45636456-45636475 None:intergenic 30.0%
!!! TATATTTTTGAGTCATGGCA+TGG - chr8.1:45637549-45637568 MS.gene34706:intron 30.0%
!!! TGTTTTTTGATGTACGTAGT+TGG - chr8.1:45637072-45637091 MS.gene34706:CDS 30.0%
AGTCCAAGTGAAATTGTGAT+TGG + chr8.1:45636814-45636833 None:intergenic 35.0%
ATTCACGTACAAGGATATCA+AGG - chr8.1:45637666-45637685 MS.gene34706:CDS 35.0%
CTACCAATCACAATTTCACT+TGG - chr8.1:45636808-45636827 MS.gene34706:CDS 35.0%
CTAGACACAACACTAATACA+TGG + chr8.1:45636387-45636406 None:intergenic 35.0%
CTGACATATATTCACGTACA+AGG - chr8.1:45637657-45637676 MS.gene34706:CDS 35.0%
CTTGTACGTGAATATATGTC+AGG + chr8.1:45637659-45637678 None:intergenic 35.0%
TACGTGAATATATGTCAGGT+GGG + chr8.1:45637655-45637674 None:intergenic 35.0%
TTCGATGTAATCACAAGTGT+TGG + chr8.1:45636326-45636345 None:intergenic 35.0%
TTGTACATGATAACAAGCGA+TGG + chr8.1:45637299-45637318 None:intergenic 35.0%
! CGATGTCTGTTTTCATTTTC+CGG - chr8.1:45636039-45636058 MS.gene34706:CDS 35.0%
! CTGTTTTCATTTTCCGGATT+GGG - chr8.1:45636045-45636064 MS.gene34706:CDS 35.0%
! GACATGACTCATGAGTATTT+TGG + chr8.1:45636468-45636487 None:intergenic 35.0%
! TCTGTTTTCATTTTCCGGAT+TGG - chr8.1:45636044-45636063 MS.gene34706:CDS 35.0%
AACCACCACCATTGAAAACA+TGG - chr8.1:45637595-45637614 MS.gene34706:CDS 40.0%
AACTCTGGATACCTTAGAAC+AGG + chr8.1:45637341-45637360 None:intergenic 40.0%
ACGGAGAGAAAGGAAACTAT+TGG + chr8.1:45636684-45636703 None:intergenic 40.0%
ACTCTGGATACCTTAGAACA+GGG + chr8.1:45637340-45637359 None:intergenic 40.0%
AGAGATAGAGTTCTTCAAGG+TGG + chr8.1:45636111-45636130 None:intergenic 40.0%
AGATACAGCTTCTTCAGTGA+TGG + chr8.1:45636639-45636658 None:intergenic 40.0%
AGATAGAGTTCTTCAAGGTG+GGG + chr8.1:45636109-45636128 None:intergenic 40.0%
AGATTTCAATCTCTTGGAGC+TGG + chr8.1:45636790-45636809 None:intergenic 40.0%
CATGAGTCATGTCTTAATGC+TGG - chr8.1:45636475-45636494 MS.gene34706:intron 40.0%
GAGATAGAGTTCTTCAAGGT+GGG + chr8.1:45636110-45636129 None:intergenic 40.0%
GTACGTGAATATATGTCAGG+TGG + chr8.1:45637656-45637675 None:intergenic 40.0%
TGCTTGTACACGATAACAAC+AGG + chr8.1:45637117-45637136 None:intergenic 40.0%
TGGAGAGATAGAGTTCTTCA+AGG + chr8.1:45636114-45636133 None:intergenic 40.0%
TTCGCGTCATTTGACATCAA+AGG - chr8.1:45636995-45637014 MS.gene34706:CDS 40.0%
! CGATCTAATTTGCGAAACTC+TGG + chr8.1:45637356-45637375 None:intergenic 40.0%
! CTCGGATGTCTTTGAGAATA+GGG + chr8.1:45636950-45636969 None:intergenic 40.0%
! GACTGGCTAATGCTAGTAAT+TGG - chr8.1:45637218-45637237 MS.gene34706:intron 40.0%
! TCTCGGATGTCTTTGAGAAT+AGG + chr8.1:45636951-45636970 None:intergenic 40.0%
!! AGCACAGTGTTGACGAATAA+TGG + chr8.1:45635980-45635999 None:intergenic 40.0%
!! AGTTGATGAAGAGGGTAGAA+GGG + chr8.1:45636156-45636175 None:intergenic 40.0%
!! CATTCAGAAACTGTTGACTC+TGG + chr8.1:45637624-45637643 None:intergenic 40.0%
!! GTAATCACAAGTGTTGGTGT+CGG + chr8.1:45636320-45636339 None:intergenic 40.0%
!! GTTGTTGCAGTTGATGAAGA+GGG + chr8.1:45636164-45636183 None:intergenic 40.0%
AATGATGCCCAATGTGACAC+GGG + chr8.1:45636859-45636878 None:intergenic 45.0%
ACAATCCAGTGTTTCCAGCA+CGG + chr8.1:45636712-45636731 None:intergenic 45.0%
AGTCTTCCGCTTTATCGATG+AGG - chr8.1:45635928-45635947 MS.gene34706:CDS 45.0%
ATAGATCCCCGTGTCACATT+GGG - chr8.1:45636849-45636868 MS.gene34706:CDS 45.0%
CAATGATGCCCAATGTGACA+CGG + chr8.1:45636860-45636879 None:intergenic 45.0%
CATGATAACAAGCGATGGTG+CGG + chr8.1:45637294-45637313 None:intergenic 45.0%
CGAAAAATTCGCCTTCACCT+GGG + chr8.1:45636926-45636945 None:intergenic 45.0%
CTCTGGATACCTTAGAACAG+GGG + chr8.1:45637339-45637358 None:intergenic 45.0%
CTTCGTCCTCATCGATAAAG+CGG + chr8.1:45635937-45635956 None:intergenic 45.0%
GAAAAATTCGCCTTCACCTG+GGG + chr8.1:45636925-45636944 None:intergenic 45.0%
GAGTTCAACAGAGTCATGAG+TGG + chr8.1:45636134-45636153 None:intergenic 45.0%
TACACGATAACAACAGGCGA+TGG + chr8.1:45637111-45637130 None:intergenic 45.0%
TAGATTACCTGAGGGAAACG+AGG + chr8.1:45636186-45636205 None:intergenic 45.0%
TATAGATCCCCGTGTCACAT+TGG - chr8.1:45636848-45636867 MS.gene34706:CDS 45.0%
TATGGATCCGTACTGCTACT+GGG - chr8.1:45636083-45636102 MS.gene34706:CDS 45.0%
TCGAAAAATTCGCCTTCACC+TGG + chr8.1:45636927-45636946 None:intergenic 45.0%
TCTCATCATTCAGAGCAGCA+AGG - chr8.1:45637451-45637470 MS.gene34706:intron 45.0%
TGATGAGAACTCGAAGCTTG+TGG + chr8.1:45637439-45637458 None:intergenic 45.0%
TGTGGCATTTCTCAAGCACA+TGG + chr8.1:45637421-45637440 None:intergenic 45.0%
TTATGGATCCGTACTGCTAC+TGG - chr8.1:45636082-45636101 MS.gene34706:CDS 45.0%
TTCGAATGTTGGGGAATCTG+CGG + chr8.1:45636009-45636028 None:intergenic 45.0%
TTCTGATTGAATGCGTGTCC+CGG - chr8.1:45636275-45636294 MS.gene34706:intron 45.0%
TTTCAATCTCTTGGAGCTGG+AGG + chr8.1:45636787-45636806 None:intergenic 45.0%
! TCTGGCTGCCATGTTTTCAA+TGG + chr8.1:45637606-45637625 None:intergenic 45.0%
! TGCCATGTTTTCAATGGTGG+TGG + chr8.1:45637600-45637619 None:intergenic 45.0%
!! AAGTTATAACGGGCAGGAAG+TGG + chr8.1:45637144-45637163 None:intergenic 45.0%
!! AGTTATAACGGGCAGGAAGT+GGG + chr8.1:45637143-45637162 None:intergenic 45.0%
!! CAGTTGATGAAGAGGGTAGA+AGG + chr8.1:45636157-45636176 None:intergenic 45.0%
!! CTGGATAAGTTATAACGGGC+AGG + chr8.1:45637150-45637169 None:intergenic 45.0%
!! GGTTGTTGCAGTTGATGAAG+AGG + chr8.1:45636165-45636184 None:intergenic 45.0%
AATCCAGTGTTTCCAGCACG+GGG + chr8.1:45636710-45636729 None:intergenic 50.0%
AATCTCTTGGAGCTGGAGGA+AGG + chr8.1:45636783-45636802 None:intergenic 50.0%
AATGTTGGGGAATCTGCGGA+GGG + chr8.1:45636005-45636024 None:intergenic 50.0%
ATGATGCCCAATGTGACACG+GGG + chr8.1:45636858-45636877 None:intergenic 50.0%
CAATCCAGTGTTTCCAGCAC+GGG + chr8.1:45636711-45636730 None:intergenic 50.0%
CACTTTCTGCCCCTGTTCTA+AGG - chr8.1:45637327-45637346 MS.gene34706:intron 50.0%
CGTGTCACATTGGGCATCAT+TGG - chr8.1:45636858-45636877 MS.gene34706:CDS 50.0%
CTTAGAACAGGGGCAGAAAG+TGG + chr8.1:45637329-45637348 None:intergenic 50.0%
GTCTAGCGTGTGTGCTTCAT+AGG - chr8.1:45636401-45636420 MS.gene34706:CDS 50.0%
TCTACTCGACTTCGATGCTG+TGG - chr8.1:45636656-45636675 MS.gene34706:intron 50.0%
TGGTGTCTCTTGTTGCTGGT+AGG - chr8.1:45637569-45637588 MS.gene34706:CDS 50.0%
TGTCTCTTGTTGCTGGTAGG+AGG - chr8.1:45637572-45637591 MS.gene34706:CDS 50.0%
TTTCTCGATGTCCTTTCCCC+AGG - chr8.1:45636906-45636925 MS.gene34706:CDS 50.0%
! GGCTGCCATGTTTTCAATGG+TGG + chr8.1:45637603-45637622 None:intergenic 50.0%
! TGAGTCTTGTGTCTCGTAGG+TGG - chr8.1:45635882-45635901 MS.gene34706:CDS 50.0%
!! TTTGCGGAACGGGCTTGTTT+TGG - chr8.1:45637722-45637741 MS.gene34706:CDS 50.0%
AGACTTGTAGCTGCTGCCAG+AGG + chr8.1:45635913-45635932 None:intergenic 55.0%
ATTCGCCTTCACCTGGGGAA+AGG + chr8.1:45636920-45636939 None:intergenic 55.0%
GAATGTTGGGGAATCTGCGG+AGG + chr8.1:45636006-45636025 None:intergenic 55.0%
GATGTCCTTTCCCCAGGTGA+AGG - chr8.1:45636912-45636931 MS.gene34706:CDS 55.0%
GCAACAACCTCGTTTCCCTC+AGG - chr8.1:45636176-45636195 MS.gene34706:intron 55.0%
GGAAGGTGGAACAGGAACGT+TGG + chr8.1:45636766-45636785 None:intergenic 55.0%
GGCATGGTGTCTCTTGTTGC+TGG - chr8.1:45637565-45637584 MS.gene34706:CDS 55.0%
ACACAGCGCGTGTCAGACAC+CGG + chr8.1:45636297-45636316 None:intergenic 60.0%
CTCTTGGAGCTGGAGGAAGG+TGG + chr8.1:45636780-45636799 None:intergenic 60.0%
CTTGTAGCTGCTGCCAGAGG+TGG + chr8.1:45635910-45635929 None:intergenic 60.0%
GAGCTGGAGGAAGGTGGAAC+AGG + chr8.1:45636774-45636793 None:intergenic 60.0%
TGTTTCCAGCACGGGGCAGA+CGG + chr8.1:45636703-45636722 None:intergenic 60.0%
CACAGCGCGTGTCAGACACC+GGG + chr8.1:45636296-45636315 None:intergenic 65.0%
CACGGGGCAGACGGAGAGAA+AGG + chr8.1:45636694-45636713 None:intergenic 65.0%
CTGCCCCGTGCTGGAAACAC+TGG - chr8.1:45636704-45636723 MS.gene34706:intron 65.0%
GGTGGGGCCCAGTAGCAGTA+CGG + chr8.1:45636093-45636112 None:intergenic 65.0%
CTCGTAGGTGGCGCCACCTC+TGG - chr8.1:45635894-45635913 MS.gene34706:CDS 70.0%
TCTCTCCGTCTGCCCCGTGC+TGG - chr8.1:45636695-45636714 MS.gene34706:intron 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 45635881 45637875 45635881 ID=MS.gene34706
chr8.1 mRNA 45635881 45637875 45635881 ID=MS.gene34706.t1;Parent=MS.gene34706
chr8.1 exon 45637559 45637875 45637559 ID=MS.gene34706.t1.exon1;Parent=MS.gene34706.t1
chr8.1 CDS 45637559 45637875 45637559 ID=cds.MS.gene34706.t1;Parent=MS.gene34706.t1
chr8.1 exon 45636740 45637172 45636740 ID=MS.gene34706.t1.exon2;Parent=MS.gene34706.t1
chr8.1 CDS 45636740 45637172 45636740 ID=cds.MS.gene34706.t1;Parent=MS.gene34706.t1
chr8.1 exon 45636284 45636433 45636284 ID=MS.gene34706.t1.exon3;Parent=MS.gene34706.t1
chr8.1 CDS 45636284 45636433 45636284 ID=cds.MS.gene34706.t1;Parent=MS.gene34706.t1
chr8.1 exon 45635881 45636165 45635881 ID=MS.gene34706.t1.exon4;Parent=MS.gene34706.t1
chr8.1 CDS 45635881 45636165 45635881 ID=cds.MS.gene34706.t1;Parent=MS.gene34706.t1
Gene Sequence

>MS.gene34706

ATGAGTCTTGTGTCTCGTAGGTGGCGCCACCTCTGGCAGCAGCTACAAGTCTTCCGCTTTATCGATGAGGACGAAGACGTAGAGTTCAATGACTTTCCATTATTCGTCAACACTGTGCTCACCCTCCGCAGATTCCCCAACATTCGAAAATTTTGTCTCGATGTCTGTTTTCATTTTCCGGATTGGGAAAACATGTTTAAGTTATGGATCCGTACTGCTACTGGGCCCCACCTTGAAGAACTCTATCTCTCCACTCATGACTCTGTTGAACTCCCTTCTACCCTCTTCATCAACTGCAACAACCTCGTTTCCCTCAGGTAATCTAATTTGTTTTCGCTAATAGCTAGTTTCATTCAAAAACCTTTAAATAAGTAGAATTGGTTTGCACGGAGACTTCTGATTGAATGCGTGTCCCGGTGTCTGACACGCGCTGTGTCCGACACCAACACTTGTGATTACATCGAATTATTTAGTTTTCCAAATTTTTATCGGTGTCAATGTGTCCATGTATTAGTGTTGTGTCTAGCGTGTGTGCTTCATAGGTCTGACTTCTGTTTCAAAATTGTTTTAATCCTATGAAATTGCCAAAATACTCATGAGTCATGTCTTAATGCTGGAAATCAATTTTGGTATAAATTTTACATATTATCTTCAAATAGACAATGTTATATATAATCTCTCTTGTCTCTTGAAAACACTGCAGTCTTACGTGTGTAGAAATCAAAATTAAAGTTCGACATTGTTCATTTCATTTTCCATCACTGAAGAAGCTGTATCTACTCGACTTCGATGCTGTGGATCCAATAGTTTCCTTTCTCTCCGTCTGCCCCGTGCTGGAAACACTGGATTGTGATTACTTTTGCTATAAAGACATATCTTTGACCAACGTTCCTGTTCCACCTTCCTCCAGCTCCAAGAGATTGAAATCTACCAATCACAATTTCACTTGGACTTATTTTAAGTTAGATATAGATCCCCGTGTCACATTGGGCATCATTGGAAACTTTAATAATATGGTGGAAGCATTTCTCGATGTCCTTTCCCCAGGTGAAGGCGAATTTTTCGACCCTATTCTCAAAGACATCCGAGATGACCAATATGATATTTCTCTACTTTCGCGTCATTTGACATCAAAGGTGAAGTTTTTATTTTTGAATGGAAAATGTTAGTAATTAGTTCTTATGGTTTATATGTTTTTTGATGTACGTAGTTGGTACTAATATTGCACCATCGCCTGTTGTTATCGTGTACAAGCAGTGCCCACTTCCTGCCCGTTATAACTTATCCAGAATTTGGAAATTTACTTCATCTAAATTTATTTCATTGTTTTTATTTTTGACTGGCTAATGCTAGTAATTGGTTGTTATGCATTATATGTTTTTGATATACGTAGTTGGTACTAATATTGCACCGCACCATCGCTTGTTATCATGTACAATCAGTGGCCACTTTCTGCCCCTGTTCTAAGGTATCCAGAGTTTCGCAAATTAGATCGTCTAAAGTTCATTCTTCCATGTTTCAACTCAAATCTTCTGGTCCATGTGCTTGAGAAATGCCACAAGCTTCGAGTTCTCATCATTCAGAGCAGCAAGGTATGTTGACTTCTCAAGTTTAATTTAATATATGTCTGGTTTGTCTATATATGCATGCAATCTATCTGTGCAAAAATATATTTTTGAGTCATGGCATGGTGTCTCTTGTTGCTGGTAGGAGGAACCACCACCATTGAAAACATGGCAGCCAGAGTCAACAGTTTCTGAATGTCTCAAATCCCACCTGACATATATTCACGTACAAGGATATCAAGGACTTGAAGATGAGTTAACATTTGTTGAATATATTTTGCGGAACGGGCTTGTTTTGGAGACAGTGCTTATTTTCTTTGATATTTCAATGGACAAAACAAACAAGGACTGCTCGTTCAAAAGATTATCTGAGATAACAATTGGTTCTTCCACGTGTCAACTTAAATTTAACCATGTTATATCTCCTTAA
Protein sequence

>MS.gene34706.t1

MSLVSRRWRHLWQQLQVFRFIDEDEDVEFNDFPLFVNTVLTLRRFPNIRKFCLDVCFHFPDWENMFKLWIRTATGPHLEELYLSTHDSVELPSTLFINCNNLVSLSLTCVEIKIKVRHCSFHFPSLKKLYLLDFDAVDPIVSFLSVCPVLETLDCDYFCYKDISLTNVPVPPSSSSKRLKSTNHNFTWTYFKLDIDPRVTLGIIGNFNNMVEAFLDVLSPGEGEFFDPILKDIRDDQYDISLLSRHLTSKWPLSAPVLRYPEFRKLDRLKFILPCFNSNLLVHVLEKCHKLRVLIIQSSKEEPPPLKTWQPESTVSECLKSHLTYIHVQGYQGLEDELTFVEYILRNGLVLETVLIFFDISMDKTNKDCSFKRLSEITIGSSTCQLKFNHVISP