AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63257

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63257.t1 MTR_8g070950 64.775 423 122 11 1 408 29 439 1.10e-173 492
MS.gene63257.t1 MTR_8g062010 57.794 417 161 8 1 407 23 434 1.83e-141 410
MS.gene63257.t1 MTR_8g062310 56.204 411 161 11 1 408 39 433 1.00e-139 406
MS.gene63257.t1 MTR_8g062300 53.915 447 161 11 1 407 40 481 1.09e-136 400
MS.gene63257.t1 MTR_8g469020 52.691 446 163 14 1 408 21 456 7.62e-136 397
MS.gene63257.t1 MTR_8g062080 54.567 427 160 15 1 407 37 449 2.32e-134 393
MS.gene63257.t1 MTR_8g058965 52.809 445 161 13 1 407 27 460 6.04e-128 377
MS.gene63257.t1 MTR_8g467790 54.331 381 112 13 1 357 162 504 4.56e-114 343
MS.gene63257.t1 MTR_4g050250 49.118 397 143 15 1 349 35 420 7.19e-102 309
MS.gene63257.t1 MTR_2g083840 39.954 433 220 15 1 403 35 457 2.35e-67 221
MS.gene63257.t1 MTR_2g086020 73.973 146 37 1 264 408 175 320 1.12e-66 215
MS.gene63257.t1 MTR_2g086020 46.043 139 65 5 15 145 1 137 1.42e-23 100
MS.gene63257.t1 MTR_8g014260 38.372 430 218 16 8 403 35 451 6.53e-62 206
MS.gene63257.t1 MTR_8g062270 36.986 438 226 14 1 402 30 453 2.82e-61 205
MS.gene63257.t1 MTR_8g468810 39.367 442 212 22 1 403 39 463 7.92e-61 204
MS.gene63257.t1 MTR_8g467800 38.128 438 228 18 1 408 34 458 2.81e-58 197
MS.gene63257.t1 MTR_5g092960 36.323 446 225 18 1 403 33 462 3.35e-58 197
MS.gene63257.t1 MTR_8g058970 35.730 459 225 21 1 403 33 477 6.87e-55 189
MS.gene63257.t1 MTR_8g468840 36.674 439 226 19 1 403 28 450 5.13e-54 186
MS.gene63257.t1 MTR_4g032730 35.135 444 232 15 7 403 33 467 1.42e-53 185
MS.gene63257.t1 MTR_8g468760 36.530 438 200 16 1 402 23 418 6.82e-52 179
MS.gene63257.t1 MTR_8g445780 37.867 375 193 17 7 356 31 390 3.43e-50 174
MS.gene63257.t1 MTR_8g468810 39.255 349 161 19 1 314 39 371 6.63e-41 149
MS.gene63257.t1 MTR_5g092960 36.080 352 174 14 1 314 33 371 1.62e-39 145
MS.gene63257.t1 MTR_8g014160 31.776 428 210 17 1 403 55 425 1.72e-38 144
MS.gene63257.t1 MTR_4g024710 36.735 294 163 11 125 403 62 347 7.37e-36 135
MS.gene63257.t1 MTR_8g468810 41.176 272 117 16 1 244 39 295 1.51e-34 130
MS.gene63257.t1 MTR_8g468810 41.176 272 117 16 1 244 39 295 2.06e-34 130
MS.gene63257.t1 MTR_8g468810 41.176 272 117 16 1 244 39 295 3.57e-34 130
MS.gene63257.t1 MTR_8g077460 45.455 143 75 3 262 403 235 375 3.20e-30 120
MS.gene63257.t1 MTR_8g077460 34.826 201 103 11 7 187 28 220 7.25e-12 67.0
MS.gene63257.t1 MTR_8g467800 38.148 270 135 14 1 248 34 293 6.02e-27 110
MS.gene63257.t1 MTR_8g014210 36.134 238 132 10 179 403 1 231 1.09e-26 107
MS.gene63257.t1 MTR_4g024745 35.401 274 154 11 123 381 14 279 1.77e-26 107
MS.gene63257.t1 MTR_3g099800 63.158 76 26 2 316 389 19 94 1.30e-23 94.7
MS.gene63257.t1 MTR_5g084070 25.854 410 268 13 1 390 27 420 5.61e-18 85.9
MS.gene63257.t1 MTR_5g080710 26.500 400 248 13 1 375 47 425 2.83e-17 84.0
MS.gene63257.t1 MTR_5g021550 46.269 134 53 9 1 119 33 162 3.90e-17 78.6
MS.gene63257.t1 MTR_7g098510 25.238 420 233 17 3 403 34 391 1.62e-14 75.1
MS.gene63257.t1 MTR_8g014290 39.007 141 61 7 1 120 24 160 2.27e-14 70.9
MS.gene63257.t1 MTR_8g014230 43.590 78 43 1 314 391 17 93 3.24e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63257.t1 AT5G50270 27.184 412 247 15 7 388 20 408 2.23e-18 86.7
MS.gene63257.t1 AT5G56560 26.555 418 244 17 17 403 33 418 3.13e-17 83.6
MS.gene63257.t1 AT2G26030 27.640 445 251 22 7 404 22 442 9.80e-16 79.0
MS.gene63257.t1 AT2G26030 27.640 445 251 22 7 404 22 442 9.80e-16 79.0
MS.gene63257.t1 AT2G26030 27.617 449 251 23 7 404 22 447 2.89e-15 77.8
MS.gene63257.t1 AT5G56560 27.609 297 182 10 125 403 326 607 1.14e-13 73.2
MS.gene63257.t1 AT5G50270 26.613 248 151 9 7 232 20 258 7.47e-12 66.2

Find 91 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGGAAACTTTAATAATA+TGG 0.213057 8.4:-42596440 MS.gene63257:CDS
CACTTTCTGCCCCTGTTCTA+AGG 0.248751 8.4:-42595988 MS.gene63257:CDS
TTTGCGGAACGGGCTTGTTT+TGG 0.301181 8.4:-42595593 MS.gene63257:CDS
AGCACAGTGTTGACGAATAA+TGG 0.325293 8.4:+42597338 None:intergenic
CAGTTGATGAAGAGGGTAGA+AGG 0.327420 8.4:+42597161 None:intergenic
GGGAAAACATGTTTAAGTTA+TGG 0.346779 8.4:-42597250 MS.gene63257:CDS
CGTGTCACATTGGGCATCAT+TGG 0.351168 8.4:-42596457 MS.gene63257:CDS
ATTGGTAGATTTCAATCTCT+TGG 0.357522 8.4:+42596522 None:intergenic
ATGTTTCAACTCAAATCTTC+TGG 0.358687 8.4:-42595921 MS.gene63257:CDS
AACCATGTTATATCTCCTTA+AGG 0.368169 8.4:-42595460 None:intergenic
AACTCTGGATACCTTAGAAC+AGG 0.387304 8.4:+42595977 None:intergenic
CTCGGATGTCTTTGAGAATA+GGG 0.388547 8.4:+42596368 None:intergenic
CTCGTAGGTGGCGTCACCTC+TGG 0.399198 8.4:-42597421 MS.gene63257:CDS
TTATGGATCCGTACTGCTAC+TGG 0.402373 8.4:-42597233 MS.gene63257:CDS
TCTCGGATGTCTTTGAGAAT+AGG 0.404973 8.4:+42596367 None:intergenic
TCTCTCCGTCTGCCCCGTGC+TGG 0.428073 8.4:-42596620 MS.gene63257:CDS
GAGCTGGAGGAAGGTGGAAC+AGG 0.435822 8.4:+42596544 None:intergenic
CGATCTAATTTGCGAAACTC+TGG 0.442892 8.4:+42595962 None:intergenic
ACGGAGAGAAAGGAAACTAT+TGG 0.444858 8.4:+42596634 None:intergenic
ATAGATCCCCGTGTCACATT+GGG 0.455209 8.4:-42596466 MS.gene63257:CDS
CACGGGGCAGACGGAGAGAA+AGG 0.456042 8.4:+42596624 None:intergenic
AAAGTAGAGAAATATCATAT+TGG 0.462773 8.4:+42596341 None:intergenic
CTACCAATCACAATTTCACT+TGG 0.468189 8.4:-42596507 MS.gene63257:CDS
CTGCCCCGTGCTGGAAACAC+TGG 0.475059 8.4:-42596611 MS.gene63257:CDS
AGATTTCAATCTCTTGGAGC+TGG 0.476427 8.4:+42596528 None:intergenic
ACAGAGGTAATGGTCGGACT+CGG 0.477050 8.4:+42597464 None:intergenic
AGTTGATGAAGAGGGTAGAA+GGG 0.486405 8.4:+42597162 None:intergenic
CTTCGTCCTCATCGATAAAG+TGG 0.487580 8.4:+42597381 None:intergenic
CTTGTACGTGAATATATGTC+AGG 0.499325 8.4:+42595659 None:intergenic
AGTCCAAGTGAAATTGTGAT+TGG 0.501196 8.4:+42596504 None:intergenic
CAATCCAGTGTTTCCAGCAC+GGG 0.503073 8.4:+42596607 None:intergenic
GAGATAGAGTTCTTCAAGGT+GGG 0.506876 8.4:+42597208 None:intergenic
CATAGTCCTCACAGAGGTAA+TGG 0.510118 8.4:+42597454 None:intergenic
CACCTTAAGGAGATATAACA+TGG 0.521086 8.4:+42595458 None:intergenic
AATCTCTTGGAGCTGGAGGA+AGG 0.531230 8.4:+42596535 None:intergenic
AGATTATCTGAGATAACAAT+GGG 0.532377 8.4:-42595508 MS.gene63257:CDS
AATGGTCGGACTCGGGAGGA+AGG 0.534339 8.4:+42597472 None:intergenic
TTCGAATGTTGCGGAATCTG+CGG 0.534607 8.4:+42597309 None:intergenic
TGTTTCCAGCACGGGGCAGA+CGG 0.535232 8.4:+42596615 None:intergenic
TATAGATCCCCGTGTCACAT+TGG 0.538369 8.4:-42596467 MS.gene63257:CDS
AAATATCATATTGGTCATCT+CGG 0.543175 8.4:+42596350 None:intergenic
TGGAGAGATAGAGTTCTTCA+AGG 0.545549 8.4:+42597204 None:intergenic
AATGATGCCCAATGTGACAC+GGG 0.545808 8.4:+42596459 None:intergenic
CTTAGAACAGGGGCAGAAAG+TGG 0.548297 8.4:+42595989 None:intergenic
TTCGCGTCATTTGACATCAA+AGG 0.548380 8.4:-42596320 MS.gene63257:intron
GAATGTTGCGGAATCTGCGG+AGG 0.548724 8.4:+42597312 None:intergenic
ATTCACGTACAAGGATATCA+AGG 0.550700 8.4:-42595649 MS.gene63257:CDS
AGATACAGCTTCTTCAGTGA+TGG 0.555293 8.4:+42596679 None:intergenic
ACCATTGAAAACATGGCAGC+CGG 0.556462 8.4:-42595713 MS.gene63257:CDS
TTTCAATCTCTTGGAGCTGG+AGG 0.561546 8.4:+42596531 None:intergenic
GCAACAACCTCGTTTCCCTC+AGG 0.563749 8.4:-42597139 MS.gene63257:intron
CAATGATGCCCAATGTGACA+CGG 0.564076 8.4:+42596458 None:intergenic
CTATGAGTCTTGTGTCTCGT+AGG 0.564360 8.4:-42597436 MS.gene63257:CDS
CAGAGGTAATGGTCGGACTC+GGG 0.570050 8.4:+42597465 None:intergenic
CTGACATATATTCACGTACA+AGG 0.572025 8.4:-42595658 MS.gene63257:CDS
AAGATTATCTGAGATAACAA+TGG 0.576292 8.4:-42595509 MS.gene63257:CDS
TATGGATCCGTACTGCTACT+GGG 0.576454 8.4:-42597232 MS.gene63257:CDS
ACAATCCAGTGTTTCCAGCA+CGG 0.581383 8.4:+42596606 None:intergenic
GGTTGTTGCAGTTGATGAAG+AGG 0.585577 8.4:+42597153 None:intergenic
TGGAAACTTTAATAATATGG+TGG 0.586549 8.4:-42596437 MS.gene63257:CDS
AGAAGATTTGAGTTGAAACA+TGG 0.586977 8.4:+42595923 None:intergenic
GGTGGGGCCCAGTAGCAGTA+CGG 0.597691 8.4:+42597225 None:intergenic
AACCACCACCATTGAAAACA+TGG 0.601661 8.4:-42595720 MS.gene63257:CDS
TCTACTCGACTTCGATGCTG+TGG 0.604755 8.4:-42596659 MS.gene63257:CDS
TGAGTCTTGTGTCTCGTAGG+TGG 0.607225 8.4:-42597433 MS.gene63257:CDS
AGATAGAGTTCTTCAAGGTG+GGG 0.613840 8.4:+42597209 None:intergenic
GTTGTTGCAGTTGATGAAGA+GGG 0.613860 8.4:+42597154 None:intergenic
TGTGGCATTTCTCAAGCACA+TGG 0.616958 8.4:+42595897 None:intergenic
CTCTTGGAGCTGGAGGAAGG+TGG 0.621502 8.4:+42596538 None:intergenic
CATTCAGAAACTGTTGACTC+CGG 0.626506 8.4:+42595694 None:intergenic
GTACGTGAATATATGTCAGG+TGG 0.626657 8.4:+42595662 None:intergenic
TACGTGAATATATGTCAGGT+GGG 0.631686 8.4:+42595663 None:intergenic
GAGTTCAACAGAGTCATGAG+TGG 0.635043 8.4:+42597184 None:intergenic
GTCCTCACAGAGGTAATGGT+CGG 0.636486 8.4:+42597458 None:intergenic
AGAGATAGAGTTCTTCAAGG+TGG 0.653042 8.4:+42597207 None:intergenic
AGTCTTCCACTTTATCGATG+AGG 0.655384 8.4:-42597387 MS.gene63257:CDS
TGATGAGAACTCGAAGCTTG+TGG 0.658774 8.4:+42595879 None:intergenic
AGGTAATGGTCGGACTCGGG+AGG 0.661364 8.4:+42597468 None:intergenic
TTCAATGGACAAAACAAACA+AGG 0.664096 8.4:-42595545 MS.gene63257:CDS
GGAAGGTGGAACAGGAACGT+TGG 0.667790 8.4:+42596552 None:intergenic
AATGTTGCGGAATCTGCGGA+GGG 0.670838 8.4:+42597313 None:intergenic
TAGATTACCTGAGGGAAACG+AGG 0.676068 8.4:+42597132 None:intergenic
AATCCAGTGTTTCCAGCACG+GGG 0.676114 8.4:+42596608 None:intergenic
GTTAAATTTAAGTTGACACG+TGG 0.682285 8.4:+42595480 None:intergenic
TCTCATCATTCAGAGCAGCA+AGG 0.683961 8.4:-42595864 MS.gene63257:intron
AGACTTGTAGCTGCTGCCAG+AGG 0.688305 8.4:+42597405 None:intergenic
ACTCTGGATACCTTAGAACA+GGG 0.693635 8.4:+42595978 None:intergenic
GTCCGACCATTACCTCTGTG+AGG 0.701738 8.4:-42597460 MS.gene63257:CDS
CTCTGGATACCTTAGAACAG+GGG 0.704828 8.4:+42595979 None:intergenic
ATGATGCCCAATGTGACACG+GGG 0.739109 8.4:+42596460 None:intergenic
AAGACTCATAGTCCTCACAG+AGG 0.808089 8.4:+42597448 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATATATTTTTGAGTCA+TGG - chr8.4:42597167-42597186 MS.gene63257:CDS 15.0%
!!! AGTTTAATTTAATATATCTC+TGG - chr8.4:42597112-42597131 MS.gene63257:intron 15.0%
!!! TATTTTCTTTGATATTTCAA+TGG - chr8.4:42597378-42597397 MS.gene63257:CDS 15.0%
!!! TTAGTTTTCCAAATTTTTAT+CGG - chr8.4:42595973-42595992 MS.gene63257:CDS 15.0%
!! AAAGTAGAGAAATATCATAT+TGG + chr8.4:42596600-42596619 None:intergenic 20.0%
!! AAATTCTGGATAAGTTATAA+CGG + chr8.4:42596778-42596797 None:intergenic 20.0%
!! AACCAATTCTACTTATTTAA+AGG + chr8.4:42595867-42595886 None:intergenic 20.0%
!! ATCCTTTAAATAAGTAGAAT+TGG - chr8.4:42595862-42595881 MS.gene63257:intron 20.0%
!! CATTGGAAACTTTAATAATA+TGG - chr8.4:42596498-42596517 MS.gene63257:CDS 20.0%
!!! ATGTTAGTAATTAGTTCTTA+TGG - chr8.4:42596666-42596685 MS.gene63257:CDS 20.0%
!!! CATTTGTTGAATATATTTTG+CGG - chr8.4:42597329-42597348 MS.gene63257:CDS 20.0%
!!! GTGAAGTTTTTATTTTTGAA+TGG - chr8.4:42596640-42596659 MS.gene63257:CDS 20.0%
!!! TCATTGTTTTTATTTTTGAC+TGG - chr8.4:42596824-42596843 MS.gene63257:intron 20.0%
! AAATATCATATTGGTCATCT+CGG + chr8.4:42596591-42596610 None:intergenic 25.0%
! AATTCTGGATAAGTTATAAC+GGG + chr8.4:42596777-42596796 None:intergenic 25.0%
! GTTATAACTTATCCAGAATT+TGG - chr8.4:42596777-42596796 MS.gene63257:intron 25.0%
! TGAAGTAAATTTCCAAATTC+TGG + chr8.4:42596792-42596811 None:intergenic 25.0%
! TGGAAACTTTAATAATATGG+TGG - chr8.4:42596501-42596520 MS.gene63257:CDS 25.0%
!! AAGATTATCTGAGATAACAA+TGG - chr8.4:42597429-42597448 MS.gene63257:CDS 25.0%
!! AGATTATCTGAGATAACAAT+GGG - chr8.4:42597430-42597449 MS.gene63257:CDS 25.0%
!! TTAATGCTGGAAATCAATTT+TGG - chr8.4:42596111-42596130 MS.gene63257:intron 25.0%
!!! ATGTTTTTGATATACGTAGT+TGG - chr8.4:42596878-42596897 MS.gene63257:intron 25.0%
AAAACAACTTAGATTACCTG+AGG + chr8.4:42595818-42595837 None:intergenic 30.0%
AAACAACTTAGATTACCTGA+GGG + chr8.4:42595817-42595836 None:intergenic 30.0%
ATGTTTCAACTCAAATCTTC+TGG - chr8.4:42597017-42597036 MS.gene63257:intron 30.0%
ATTGGTAGATTTCAATCTCT+TGG + chr8.4:42596419-42596438 None:intergenic 30.0%
CATTGACACCGATAAAAATT+TGG + chr8.4:42595984-42596003 None:intergenic 30.0%
TTCAATGGACAAAACAAACA+AGG - chr8.4:42597393-42597412 MS.gene63257:CDS 30.0%
! GACAAAATTTTCGAATGTTG+CGG + chr8.4:42595641-42595660 None:intergenic 30.0%
! GGGAAAACATGTTTAAGTTA+TGG - chr8.4:42595688-42595707 MS.gene63257:CDS 30.0%
! GTTGAATATATTTTGCGGAA+CGG - chr8.4:42597334-42597353 MS.gene63257:CDS 30.0%
! TTAAACATGTTTTCCCAATC+CGG + chr8.4:42595684-42595703 None:intergenic 30.0%
! TTGAATATATTTTGCGGAAC+GGG - chr8.4:42597335-42597354 MS.gene63257:CDS 30.0%
!! AGAAGATTTGAGTTGAAACA+TGG + chr8.4:42597018-42597037 None:intergenic 30.0%
!! ATAAGTAGAATTGGTTTGCA+CGG - chr8.4:42595871-42595890 MS.gene63257:CDS 30.0%
!! GTTAAATTTAAGTTGACACG+TGG + chr8.4:42597461-42597480 None:intergenic 30.0%
!!! GAGTATTTTGGCAATTTCAT+AGG + chr8.4:42596079-42596098 None:intergenic 30.0%
!!! TATATTTTTGAGTCATGGCA+TGG - chr8.4:42597172-42597191 MS.gene63257:CDS 30.0%
!!! TGTTTTTTGATGTACGTAGT+TGG - chr8.4:42596695-42596714 MS.gene63257:CDS 30.0%
AGTCCAAGTGAAATTGTGAT+TGG + chr8.4:42596437-42596456 None:intergenic 35.0%
ATTCACGTACAAGGATATCA+AGG - chr8.4:42597289-42597308 MS.gene63257:CDS 35.0%
CTACCAATCACAATTTCACT+TGG - chr8.4:42596431-42596450 MS.gene63257:CDS 35.0%
CTAGACACAACACTAATACA+TGG + chr8.4:42596010-42596029 None:intergenic 35.0%
CTGACATATATTCACGTACA+AGG - chr8.4:42597280-42597299 MS.gene63257:CDS 35.0%
CTTGTACGTGAATATATGTC+AGG + chr8.4:42597282-42597301 None:intergenic 35.0%
TACGTGAATATATGTCAGGT+GGG + chr8.4:42597278-42597297 None:intergenic 35.0%
TTGTACATGATAACAAGCGA+TGG + chr8.4:42596922-42596941 None:intergenic 35.0%
TTGTTATCATGTACAAGCAG+TGG - chr8.4:42596926-42596945 MS.gene63257:intron 35.0%
! CGATGTCTGTTTTCATTTTC+CGG - chr8.4:42595662-42595681 MS.gene63257:CDS 35.0%
! CTGTTTTCATTTTCCGGATT+GGG - chr8.4:42595668-42595687 MS.gene63257:CDS 35.0%
! GACATGACTCATGAGTATTT+TGG + chr8.4:42596091-42596110 None:intergenic 35.0%
! TCTGTTTTCATTTTCCGGAT+TGG - chr8.4:42595667-42595686 MS.gene63257:CDS 35.0%
AACCACCACCATTGAAAACA+TGG - chr8.4:42597218-42597237 MS.gene63257:CDS 40.0%
AACTCTGGATACCTTAGAAC+AGG + chr8.4:42596964-42596983 None:intergenic 40.0%
ACGGAGAGAAAGGAAACTAT+TGG + chr8.4:42596307-42596326 None:intergenic 40.0%
ACTCTGGATACCTTAGAACA+GGG + chr8.4:42596963-42596982 None:intergenic 40.0%
AGAGATAGAGTTCTTCAAGG+TGG + chr8.4:42595734-42595753 None:intergenic 40.0%
AGATACAGCTTCTTCAGTGA+TGG + chr8.4:42596262-42596281 None:intergenic 40.0%
AGATAGAGTTCTTCAAGGTG+GGG + chr8.4:42595732-42595751 None:intergenic 40.0%
AGATTTCAATCTCTTGGAGC+TGG + chr8.4:42596413-42596432 None:intergenic 40.0%
AGTCTTCCACTTTATCGATG+AGG - chr8.4:42595551-42595570 MS.gene63257:CDS 40.0%
CATGAGTCATGTCTTAATGC+TGG - chr8.4:42596098-42596117 MS.gene63257:intron 40.0%
GAGATAGAGTTCTTCAAGGT+GGG + chr8.4:42595733-42595752 None:intergenic 40.0%
GTACGTGAATATATGTCAGG+TGG + chr8.4:42597279-42597298 None:intergenic 40.0%
TGCTTGTACACGATAACAAC+AGG + chr8.4:42596740-42596759 None:intergenic 40.0%
TGGAGAGATAGAGTTCTTCA+AGG + chr8.4:42595737-42595756 None:intergenic 40.0%
TTCGATGCAATCACAAGTGT+TGG + chr8.4:42595949-42595968 None:intergenic 40.0%
TTCGCGTCATTTGACATCAA+AGG - chr8.4:42596618-42596637 MS.gene63257:CDS 40.0%
! CGATCTAATTTGCGAAACTC+TGG + chr8.4:42596979-42596998 None:intergenic 40.0%
! CTCGGATGTCTTTGAGAATA+GGG + chr8.4:42596573-42596592 None:intergenic 40.0%
! GACTGGCTAATGCTAGTAAT+TGG - chr8.4:42596841-42596860 MS.gene63257:intron 40.0%
! TCTCGGATGTCTTTGAGAAT+AGG + chr8.4:42596574-42596593 None:intergenic 40.0%
!! AGCACAGTGTTGACGAATAA+TGG + chr8.4:42595603-42595622 None:intergenic 40.0%
!! AGTTGATGAAGAGGGTAGAA+GGG + chr8.4:42595779-42595798 None:intergenic 40.0%
!! CATTCAGAAACTGTTGACTC+CGG + chr8.4:42597247-42597266 None:intergenic 40.0%
!! GTTGTTGCAGTTGATGAAGA+GGG + chr8.4:42595787-42595806 None:intergenic 40.0%
AAGACTCATAGTCCTCACAG+AGG + chr8.4:42595493-42595512 None:intergenic 45.0%
AATGATGCCCAATGTGACAC+GGG + chr8.4:42596482-42596501 None:intergenic 45.0%
ACAATCCAGTGTTTCCAGCA+CGG + chr8.4:42596335-42596354 None:intergenic 45.0%
ACCATTGAAAACATGGCAGC+CGG - chr8.4:42597225-42597244 MS.gene63257:CDS 45.0%
ATAGATCCCCGTGTCACATT+GGG - chr8.4:42596472-42596491 MS.gene63257:CDS 45.0%
CAATGATGCCCAATGTGACA+CGG + chr8.4:42596483-42596502 None:intergenic 45.0%
CATAGTCCTCACAGAGGTAA+TGG + chr8.4:42595487-42595506 None:intergenic 45.0%
CATGATAACAAGCGATGGTG+CGG + chr8.4:42596917-42596936 None:intergenic 45.0%
CGAAAAATTCGCCTTCACCT+GGG + chr8.4:42596549-42596568 None:intergenic 45.0%
CTCTGGATACCTTAGAACAG+GGG + chr8.4:42596962-42596981 None:intergenic 45.0%
CTTCGTCCTCATCGATAAAG+TGG + chr8.4:42595560-42595579 None:intergenic 45.0%
GAAAAATTCGCCTTCACCTG+GGG + chr8.4:42596548-42596567 None:intergenic 45.0%
GAGTTCAACAGAGTCATGAG+TGG + chr8.4:42595757-42595776 None:intergenic 45.0%
TACACGATAACAACAGGCGA+TGG + chr8.4:42596734-42596753 None:intergenic 45.0%
TAGATTACCTGAGGGAAACG+AGG + chr8.4:42595809-42595828 None:intergenic 45.0%
TATAGATCCCCGTGTCACAT+TGG - chr8.4:42596471-42596490 MS.gene63257:CDS 45.0%
TATGGATCCGTACTGCTACT+GGG - chr8.4:42595706-42595725 MS.gene63257:CDS 45.0%
TCGAAAAATTCGCCTTCACC+TGG + chr8.4:42596550-42596569 None:intergenic 45.0%
TCTCATCATTCAGAGCAGCA+AGG - chr8.4:42597074-42597093 MS.gene63257:intron 45.0%
TGATGAGAACTCGAAGCTTG+TGG + chr8.4:42597062-42597081 None:intergenic 45.0%
TGTGGCATTTCTCAAGCACA+TGG + chr8.4:42597044-42597063 None:intergenic 45.0%
TTATGGATCCGTACTGCTAC+TGG - chr8.4:42595705-42595724 MS.gene63257:CDS 45.0%
TTCGAATGTTGCGGAATCTG+CGG + chr8.4:42595632-42595651 None:intergenic 45.0%
TTCTGATTGAATGCGTGTCC+CGG - chr8.4:42595898-42595917 MS.gene63257:CDS 45.0%
TTTCAATCTCTTGGAGCTGG+AGG + chr8.4:42596410-42596429 None:intergenic 45.0%
! CTATGAGTCTTGTGTCTCGT+AGG - chr8.4:42595502-42595521 MS.gene63257:CDS 45.0%
! TGCCATGTTTTCAATGGTGG+TGG + chr8.4:42597223-42597242 None:intergenic 45.0%
! TTTCTCGATGTCTTTTCCCC+AGG - chr8.4:42596529-42596548 MS.gene63257:CDS 45.0%
!! AAGTTATAACGGGCAGGAAG+TGG + chr8.4:42596767-42596786 None:intergenic 45.0%
!! AGTTATAACGGGCAGGAAGT+GGG + chr8.4:42596766-42596785 None:intergenic 45.0%
!! CAGTTGATGAAGAGGGTAGA+AGG + chr8.4:42595780-42595799 None:intergenic 45.0%
!! CTGGATAAGTTATAACGGGC+AGG + chr8.4:42596773-42596792 None:intergenic 45.0%
!! GGTTGTTGCAGTTGATGAAG+AGG + chr8.4:42595788-42595807 None:intergenic 45.0%
AATCCAGTGTTTCCAGCACG+GGG + chr8.4:42596333-42596352 None:intergenic 50.0%
AATCTCTTGGAGCTGGAGGA+AGG + chr8.4:42596406-42596425 None:intergenic 50.0%
AATGTTGCGGAATCTGCGGA+GGG + chr8.4:42595628-42595647 None:intergenic 50.0%
ATGATGCCCAATGTGACACG+GGG + chr8.4:42596481-42596500 None:intergenic 50.0%
CAATCCAGTGTTTCCAGCAC+GGG + chr8.4:42596334-42596353 None:intergenic 50.0%
CACTTTCTGCCCCTGTTCTA+AGG - chr8.4:42596950-42596969 MS.gene63257:intron 50.0%
CGTGTCACATTGGGCATCAT+TGG - chr8.4:42596481-42596500 MS.gene63257:CDS 50.0%
CTTAGAACAGGGGCAGAAAG+TGG + chr8.4:42596952-42596971 None:intergenic 50.0%
GTCTAGCGTGTGTGCTTCAT+AGG - chr8.4:42596024-42596043 MS.gene63257:intron 50.0%
TCTACTCGACTTCGATGCTG+TGG - chr8.4:42596279-42596298 MS.gene63257:intron 50.0%
TGGTGTCTCTTGTTGCTGGT+AGG - chr8.4:42597192-42597211 MS.gene63257:CDS 50.0%
TGTCTCTTGTTGCTGGTAGG+AGG - chr8.4:42597195-42597214 MS.gene63257:CDS 50.0%
! ACAGAGGTAATGGTCGGACT+CGG + chr8.4:42595477-42595496 None:intergenic 50.0%
! GATGTCTTTTCCCCAGGTGA+AGG - chr8.4:42596535-42596554 MS.gene63257:CDS 50.0%
! GGCTGCCATGTTTTCAATGG+TGG + chr8.4:42597226-42597245 None:intergenic 50.0%
! GTCCTCACAGAGGTAATGGT+CGG + chr8.4:42595483-42595502 None:intergenic 50.0%
! TCCGGCTGCCATGTTTTCAA+TGG + chr8.4:42597229-42597248 None:intergenic 50.0%
! TGAGTCTTGTGTCTCGTAGG+TGG - chr8.4:42595505-42595524 MS.gene63257:CDS 50.0%
!! TTTGCGGAACGGGCTTGTTT+TGG - chr8.4:42597345-42597364 MS.gene63257:CDS 50.0%
AGACTTGTAGCTGCTGCCAG+AGG + chr8.4:42595536-42595555 None:intergenic 55.0%
GAATGTTGCGGAATCTGCGG+AGG + chr8.4:42595629-42595648 None:intergenic 55.0%
GCAACAACCTCGTTTCCCTC+AGG - chr8.4:42595799-42595818 MS.gene63257:intron 55.0%
GGAAGGTGGAACAGGAACGT+TGG + chr8.4:42596389-42596408 None:intergenic 55.0%
GGCATGGTGTCTCTTGTTGC+TGG - chr8.4:42597188-42597207 MS.gene63257:CDS 55.0%
GTCCGACCATTACCTCTGTG+AGG - chr8.4:42595478-42595497 MS.gene63257:CDS 55.0%
! CAGAGGTAATGGTCGGACTC+GGG + chr8.4:42595476-42595495 None:intergenic 55.0%
AATGGTCGGACTCGGGAGGA+AGG + chr8.4:42595469-42595488 None:intergenic 60.0%
ACACAGCGCGTGTCAGACAC+CGG + chr8.4:42595920-42595939 None:intergenic 60.0%
CTCTTGGAGCTGGAGGAAGG+TGG + chr8.4:42596403-42596422 None:intergenic 60.0%
GAGCTGGAGGAAGGTGGAAC+AGG + chr8.4:42596397-42596416 None:intergenic 60.0%
TGTTTCCAGCACGGGGCAGA+CGG + chr8.4:42596326-42596345 None:intergenic 60.0%
! AGGTAATGGTCGGACTCGGG+AGG + chr8.4:42595473-42595492 None:intergenic 60.0%
CACAGCGCGTGTCAGACACC+GGG + chr8.4:42595919-42595938 None:intergenic 65.0%
CACGGGGCAGACGGAGAGAA+AGG + chr8.4:42596317-42596336 None:intergenic 65.0%
CTCGTAGGTGGCGTCACCTC+TGG - chr8.4:42595517-42595536 MS.gene63257:CDS 65.0%
CTGCCCCGTGCTGGAAACAC+TGG - chr8.4:42596327-42596346 MS.gene63257:CDS 65.0%
GGTGGGGCCCAGTAGCAGTA+CGG + chr8.4:42595716-42595735 None:intergenic 65.0%
TCTCTCCGTCTGCCCCGTGC+TGG - chr8.4:42596318-42596337 MS.gene63257:intron 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 42595462 42597498 42595462 ID=MS.gene63257
chr8.4 mRNA 42595462 42597498 42595462 ID=MS.gene63257.t1;Parent=MS.gene63257
chr8.4 exon 42597140 42597498 42597140 ID=MS.gene63257.t1.exon1;Parent=MS.gene63257.t1
chr8.4 CDS 42597140 42597498 42597140 ID=cds.MS.gene63257.t1;Parent=MS.gene63257.t1
chr8.4 exon 42596321 42596753 42596321 ID=MS.gene63257.t1.exon2;Parent=MS.gene63257.t1
chr8.4 CDS 42596321 42596753 42596321 ID=cds.MS.gene63257.t1;Parent=MS.gene63257.t1
chr8.4 exon 42595865 42596014 42595865 ID=MS.gene63257.t1.exon3;Parent=MS.gene63257.t1
chr8.4 CDS 42595865 42596014 42595865 ID=cds.MS.gene63257.t1;Parent=MS.gene63257.t1
chr8.4 exon 42595462 42595746 42595462 ID=MS.gene63257.t1.exon4;Parent=MS.gene63257.t1
chr8.4 CDS 42595462 42595746 42595462 ID=cds.MS.gene63257.t1;Parent=MS.gene63257.t1
Gene Sequence

>MS.gene63257

ATGTCCTTCCTCCCGAGTCCGACCATTACCTCTGTGAGGACTATGAGTCTTGTGTCTCGTAGGTGGCGTCACCTCTGGCAGCAGCTACAAGTCTTCCACTTTATCGATGAGGACGAAGACGTAGAGTTCAATGACTTTCCATTATTCGTCAACACTGTGCTCACCCTCCGCAGATTCCGCAACATTCGAAAATTTTGTCTCGATGTCTGTTTTCATTTTCCGGATTGGGAAAACATGTTTAAGTTATGGATCCGTACTGCTACTGGGCCCCACCTTGAAGAACTCTATCTCTCCACTCATGACTCTGTTGAACTCCCTTCTACCCTCTTCATCAACTGCAACAACCTCGTTTCCCTCAGGTAATCTAAGTTGTTTTCGCTAATAGCTAGTTTCATTCAAAATCCTTTAAATAAGTAGAATTGGTTTGCACGGAGACTTCTGATTGAATGCGTGTCCCGGTGTCTGACACGCGCTGTGTTCGACACCAACACTTGTGATTGCATCGAATTATTTAGTTTTCCAAATTTTTATCGGTGTCAATGTGTCCATGTATTAGTGTTGTGTCTAGCGTGTGTGCTTCATAGGTCTGACTTCTGTTTCAAAATTGTTTTAATCCTATGAAATTGCCAAAATACTCATGAGTCATGTCTTAATGCTGGAAATCAATTTTGGTATAAATTTTACATATTATCTTCAAATAGACAATGTTATATATAATCTCTCTTGTCTCTTGAAAACACTGCAGTCTTACGTGTGTAGAAATCAAAATTAAAGTTCAACATTGTTCATTTCATTTTCCATCACTGAAGAAGCTGTATCTACTCGACTTCGATGCTGTGGATCCAATAGTTTCCTTTCTCTCCGTCTGCCCCGTGCTGGAAACACTGGATTGTGATTACTTTTGCTATAAAGACATATCTTTGACCAACGTTCCTGTTCCACCTTCCTCCAGCTCCAAGAGATTGAAATCTACCAATCACAATTTCACTTGGACTTATTTTAAGTTAGATATAGATCCCCGTGTCACATTGGGCATCATTGGAAACTTTAATAATATGGTGGAAGCATTTCTCGATGTCTTTTCCCCAGGTGAAGGCGAATTTTTCGACCCTATTCTCAAAGACATCCGAGATGACCAATATGATATTTCTCTACTTTCGCGTCATTTGACATCAAAGGTGAAGTTTTTATTTTTGAATGGAAAATGTTAGTAATTAGTTCTTATGGTTTATATGTTTTTTGATGTACGTAGTTGGTACTAATATTGCACCATCGCCTGTTGTTATCGTGTACAAGCAGTGCCCACTTCCTGCCCGTTATAACTTATCCAGAATTTGGAAATTTACTTCATCTAAATTTATTTCATTGTTTTTATTTTTGACTGGCTAATGCTAGTAATTGGTTGTTATGCATTATATGTTTTTGATATACGTAGTTGGTACTAATATTGCACCGCACCATCGCTTGTTATCATGTACAAGCAGTGGCCACTTTCTGCCCCTGTTCTAAGGTATCCAGAGTTTCGCAAATTAGATCGTCTAAAGTTCATTCTTCCATGTTTCAACTCAAATCTTCTGGTCCATGTGCTTGAGAAATGCCACAAGCTTCGAGTTCTCATCATTCAGAGCAGCAAGGTATGTTGACTTCTCAAGTTTAATTTAATATATCTCTGGTTTGTCTATATATGCATGCAATCTATCTGTGCAAAAATATATTTTTGAGTCATGGCATGGTGTCTCTTGTTGCTGGTAGGAGGAACCACCACCATTGAAAACATGGCAGCCGGAGTCAACAGTTTCTGAATGTCTCAAATCCCACCTGACATATATTCACGTACAAGGATATCAAGGACTTGAAGATGAGTTAACATTTGTTGAATATATTTTGCGGAACGGGCTTGTTTTGGAGACAGTGCTTATTTTCTTTGATATTTCAATGGACAAAACAAACAAGGACTGCTCGTTCAAAAGATTATCTGAGATAACAATGGGTTCTGCCACGTGTCAACTTAAATTTAACCATGTTATATCTCCTTAA
Protein sequence

>MS.gene63257.t1

MSFLPSPTITSVRTMSLVSRRWRHLWQQLQVFHFIDEDEDVEFNDFPLFVNTVLTLRRFRNIRKFCLDVCFHFPDWENMFKLWIRTATGPHLEELYLSTHDSVELPSTLFINCNNLVSLSLTCVEIKIKVQHCSFHFPSLKKLYLLDFDAVDPIVSFLSVCPVLETLDCDYFCYKDISLTNVPVPPSSSSKRLKSTNHNFTWTYFKLDIDPRVTLGIIGNFNNMVEAFLDVFSPGEGEFFDPILKDIRDDQYDISLLSRHLTSKWPLSAPVLRYPEFRKLDRLKFILPCFNSNLLVHVLEKCHKLRVLIIQSSKEEPPPLKTWQPESTVSECLKSHLTYIHVQGYQGLEDELTFVEYILRNGLVLETVLIFFDISMDKTNKDCSFKRLSEITMGSATCQLKFNHVISP