AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34802

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34802.t1 MTR_8g062300 60.808 495 137 10 1 456 1 477 0.0 530
MS.gene34802.t1 MTR_8g070950 57.451 463 158 11 3 456 2 434 5.99e-158 454
MS.gene34802.t1 MTR_8g062010 58.186 452 160 12 12 456 1 430 6.59e-153 441
MS.gene34802.t1 MTR_8g062310 54.525 442 166 8 20 460 25 432 3.47e-149 432
MS.gene34802.t1 MTR_8g062080 53.696 460 177 11 8 456 11 445 1.92e-147 428
MS.gene34802.t1 MTR_8g469020 48.305 472 186 12 18 456 5 451 1.28e-134 395
MS.gene34802.t1 MTR_8g058965 49.061 479 188 14 11 456 1 456 5.29e-131 387
MS.gene34802.t1 MTR_4g050250 47.404 443 169 15 1 402 1 420 1.33e-109 331
MS.gene34802.t1 MTR_8g467790 45.592 397 151 11 20 401 148 494 1.18e-94 295
MS.gene34802.t1 MTR_8g468810 38.509 483 245 16 4 456 3 463 2.46e-74 241
MS.gene34802.t1 MTR_2g083840 37.553 474 249 13 6 456 8 457 1.32e-73 239
MS.gene34802.t1 MTR_8g062270 36.989 465 237 15 20 455 16 453 2.70e-72 235
MS.gene34802.t1 MTR_8g058970 37.475 491 253 17 4 456 3 477 4.23e-71 233
MS.gene34802.t1 MTR_2g086020 79.433 141 29 0 316 456 175 315 4.18e-68 220
MS.gene34802.t1 MTR_2g086020 50.000 156 73 3 45 198 1 153 2.15e-36 137
MS.gene34802.t1 MTR_8g014260 35.000 480 259 18 1 456 1 451 2.04e-66 220
MS.gene34802.t1 MTR_8g467800 35.477 482 254 15 8 460 4 457 9.37e-64 213
MS.gene34802.t1 MTR_8g468840 36.623 456 251 13 18 454 12 448 2.03e-62 209
MS.gene34802.t1 MTR_4g032730 33.538 489 258 14 8 455 4 466 6.27e-62 208
MS.gene34802.t1 MTR_5g092960 33.192 473 259 11 15 455 14 461 1.71e-58 199
MS.gene34802.t1 MTR_8g468760 34.461 473 226 15 12 455 1 418 6.87e-55 189
MS.gene34802.t1 MTR_8g468810 38.619 391 190 15 4 366 3 371 4.07e-53 182
MS.gene34802.t1 MTR_8g445780 36.207 406 215 15 20 409 13 390 8.30e-52 180
MS.gene34802.t1 MTR_8g468810 40.260 308 150 11 4 288 3 299 1.16e-44 159
MS.gene34802.t1 MTR_8g468810 40.260 308 150 11 4 288 3 299 1.59e-44 159
MS.gene34802.t1 MTR_8g468810 40.260 308 150 11 4 288 3 299 3.48e-44 158
MS.gene34802.t1 MTR_8g014160 30.853 457 224 14 20 456 41 425 4.38e-41 152
MS.gene34802.t1 MTR_5g092960 32.632 380 206 9 15 366 14 371 5.82e-38 143
MS.gene34802.t1 MTR_3g099800 70.115 87 25 1 368 453 19 105 1.13e-32 120
MS.gene34802.t1 MTR_8g467800 37.705 305 151 13 8 288 4 293 3.16e-32 125
MS.gene34802.t1 MTR_4g024710 39.500 200 104 6 258 456 164 347 8.28e-31 122
MS.gene34802.t1 MTR_8g077460 38.710 217 116 8 20 225 10 220 5.88e-30 120
MS.gene34802.t1 MTR_8g077460 41.722 151 80 5 309 456 230 375 4.15e-26 109
MS.gene34802.t1 MTR_5g021550 44.242 165 74 6 5 157 4 162 2.03e-28 110
MS.gene34802.t1 MTR_8g014210 38.500 200 102 7 260 456 50 231 2.24e-27 109
MS.gene34802.t1 MTR_1g041245 27.212 452 242 15 18 456 9 386 2.19e-25 107
MS.gene34802.t1 MTR_8g014290 39.306 173 77 7 1 158 1 160 6.65e-25 100
MS.gene34802.t1 MTR_7g026500 26.205 477 251 16 7 460 1 399 1.30e-23 102
MS.gene34802.t1 MTR_7g098510 26.170 470 254 19 1 456 1 391 2.84e-21 95.9
MS.gene34802.t1 MTR_4g024745 30.249 281 148 11 170 431 25 276 2.85e-20 90.9
MS.gene34802.t1 MTR_5g084070 26.490 453 285 18 8 448 3 419 1.25e-19 91.3
MS.gene34802.t1 MTR_4g024720 39.231 130 67 5 4 126 3 127 2.20e-18 84.0
MS.gene34802.t1 MTR_5g080710 27.191 445 262 18 3 428 24 425 3.33e-18 87.0
MS.gene34802.t1 MTR_5g073420 32.461 191 118 6 20 205 11 195 5.51e-17 82.8
MS.gene34802.t1 MTR_7g056447 32.821 195 120 6 12 204 1 186 9.72e-17 80.9
MS.gene34802.t1 MTR_7g098480 26.237 465 256 14 1 455 1 388 2.14e-16 81.6
MS.gene34802.t1 MTR_5g007410 25.459 436 263 12 22 453 28 405 9.40e-16 79.7
MS.gene34802.t1 MTR_1g108820 28.188 447 249 22 14 453 2 383 9.71e-16 79.0
MS.gene34802.t1 MTR_2g007400 24.839 467 252 18 17 456 9 403 1.05e-15 79.3
MS.gene34802.t1 MTR_2g008310 26.444 450 238 17 20 456 2 371 1.13e-15 79.0
MS.gene34802.t1 MTR_7g032380 35.385 195 107 7 20 207 11 193 1.28e-15 78.6
MS.gene34802.t1 MTR_1g053025 26.233 446 256 17 20 453 2 386 3.26e-15 77.4
MS.gene34802.t1 MTR_3g107120 32.836 201 113 10 20 205 9 202 9.48e-15 76.3
MS.gene34802.t1 MTR_4g024770 50.000 64 29 1 1 64 8 68 2.56e-14 68.2
MS.gene34802.t1 MTR_7g115270 30.137 219 125 8 8 213 5 208 2.80e-14 74.7
MS.gene34802.t1 MTR_5g008920 24.622 463 256 18 7 457 1 382 6.69e-14 73.6
MS.gene34802.t1 MTR_3g107100 30.049 203 129 9 10 208 2 195 7.75e-14 73.6
MS.gene34802.t1 MTR_2g008320 35.204 196 109 8 20 207 52 237 8.30e-14 73.6
MS.gene34802.t1 MTR_5g008950 29.524 210 133 7 22 230 1 196 1.19e-13 72.8
MS.gene34802.t1 MTR_2g007500 30.189 212 112 7 7 207 1 187 3.33e-13 70.9
MS.gene34802.t1 MTR_8g014180 55.172 58 24 1 4 61 3 58 4.47e-13 64.3
MS.gene34802.t1 MTR_3g036250 29.949 197 119 7 19 207 4 189 5.47e-13 70.5
MS.gene34802.t1 MTR_3g105060 31.980 197 122 9 20 213 14 201 6.06e-13 70.5
MS.gene34802.t1 MTR_1g492880 39.785 93 52 2 12 103 1 90 6.51e-13 65.1
MS.gene34802.t1 MTR_1g492880 39.785 93 52 2 12 103 1 90 8.14e-13 65.1
MS.gene34802.t1 MTR_7g034110 46.341 82 40 2 20 101 11 88 1.15e-12 69.3
MS.gene34802.t1 MTR_2g007450 30.357 224 132 8 9 211 5 225 1.24e-12 69.7
MS.gene34802.t1 MTR_4g098590 30.808 198 120 7 22 207 1 193 2.41e-12 68.6
MS.gene34802.t1 MTR_3g023630 30.964 197 111 8 19 205 7 188 2.99e-12 68.2
MS.gene34802.t1 MTR_3g040700 28.866 194 125 7 19 207 42 227 3.05e-12 68.6
MS.gene34802.t1 MTR_7g033990 30.000 210 117 7 17 212 8 201 3.12e-12 68.6
MS.gene34802.t1 MTR_7g056433 33.010 206 123 7 7 207 1 196 4.64e-12 67.8
MS.gene34802.t1 MTR_8g014230 40.000 85 47 2 365 449 18 98 5.84e-12 62.4
MS.gene34802.t1 MTR_8g085430 31.500 200 115 7 20 207 11 200 1.58e-11 65.9
MS.gene34802.t1 MTR_5g083890 31.884 207 110 7 20 203 6 204 1.86e-11 66.6
MS.gene34802.t1 MTR_7g055943 27.143 210 137 7 2 205 22 221 2.86e-11 65.5
MS.gene34802.t1 MTR_1g107385 31.441 229 135 10 3 216 20 241 4.96e-11 64.7
MS.gene34802.t1 MTR_3g467400 29.386 228 132 9 18 223 1 221 6.16e-11 64.3
MS.gene34802.t1 MTR_7g034115 32.474 194 118 6 18 207 9 193 7.60e-11 63.9
MS.gene34802.t1 MTR_7g033945 30.814 172 99 6 20 182 11 171 8.91e-11 61.2
MS.gene34802.t1 MTR_7g033940 33.858 127 74 5 15 139 89 207 9.26e-11 62.0
MS.gene34802.t1 MTR_4g051480 29.936 157 100 5 20 175 25 172 9.93e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34802.t1 AT4G10400 27.679 448 271 17 20 455 2 408 6.63e-22 97.8
MS.gene34802.t1 AT4G10400 27.679 448 271 17 20 455 2 408 6.63e-22 97.8
MS.gene34802.t1 AT4G10400 27.679 448 271 17 20 455 2 408 6.63e-22 97.8
MS.gene34802.t1 AT1G78750 26.415 477 301 20 4 453 2 455 1.32e-19 91.7
MS.gene34802.t1 AT1G78750 26.415 477 301 20 4 453 2 455 1.32e-19 91.7
MS.gene34802.t1 AT1G32375 27.119 472 258 19 20 456 2 422 3.99e-18 86.7
MS.gene34802.t1 AT1G32375 27.119 472 258 19 20 456 2 422 3.99e-18 86.7
MS.gene34802.t1 AT4G00160 28.139 462 282 18 20 461 16 447 5.15e-16 80.5
MS.gene34802.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.39e-15 77.8
MS.gene34802.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.39e-15 77.8
MS.gene34802.t1 AT5G50270 28.409 440 240 19 20 427 2 398 1.60e-15 78.6
MS.gene34802.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.94e-15 77.0
MS.gene34802.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.94e-15 77.0
MS.gene34802.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.94e-15 77.0
MS.gene34802.t1 AT3G50710 25.541 462 283 13 20 456 2 427 4.79e-14 74.3
MS.gene34802.t1 AT5G56440 34.392 189 113 4 20 205 2 182 9.93e-14 73.2
MS.gene34802.t1 AT4G26340 27.074 458 273 17 20 456 2 419 2.11e-13 72.4
MS.gene34802.t1 AT5G53840 24.775 444 245 17 20 426 18 409 3.62e-13 71.6
MS.gene34802.t1 AT3G59190 29.310 174 106 4 20 184 12 177 9.15e-13 70.5
MS.gene34802.t1 AT4G13965 27.115 461 261 18 19 453 9 420 1.20e-12 70.1
MS.gene34802.t1 AT3G59190 29.310 174 106 4 20 184 12 177 1.25e-12 69.7
MS.gene34802.t1 AT3G59200 30.151 199 114 7 20 205 7 193 2.48e-12 68.6
MS.gene34802.t1 AT4G13985 31.841 201 116 9 19 207 18 209 3.73e-12 68.6
MS.gene34802.t1 AT1G16930 29.126 206 127 7 8 207 3 195 4.91e-12 68.2
MS.gene34802.t1 AT1G16930 29.126 206 127 7 8 207 3 195 4.91e-12 68.2
MS.gene34802.t1 AT1G16930 29.126 206 127 7 8 207 3 195 4.91e-12 68.2
MS.gene34802.t1 AT3G59200 30.151 199 114 7 20 205 7 193 5.42e-12 68.2
MS.gene34802.t1 AT5G60610 24.941 421 257 19 20 420 2 383 5.74e-12 67.8
MS.gene34802.t1 AT5G60610 24.941 421 257 19 20 420 2 383 5.74e-12 67.8
MS.gene34802.t1 AT4G14103 31.282 195 115 5 20 205 8 192 6.02e-12 67.8
MS.gene34802.t1 AT4G14103 31.088 193 118 4 20 205 8 192 6.64e-12 67.4
MS.gene34802.t1 AT4G14103 31.088 193 118 4 20 205 8 192 6.64e-12 67.4
MS.gene34802.t1 AT1G48400 30.052 193 121 4 20 205 10 195 6.67e-12 67.8
MS.gene34802.t1 AT1G48400 30.052 193 121 4 20 205 10 195 8.71e-12 67.4
MS.gene34802.t1 AT3G52680 32.143 196 114 6 20 208 21 204 1.38e-11 66.6
MS.gene34802.t1 AT3G52680 32.143 196 114 6 20 208 21 204 2.74e-11 65.5

Find 112 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATAGTTTACCTTCTATT+TGG 0.229961 8.3:+40112575 MS.gene34802:CDS
CATTGGCAACTTTCACAGTA+TGG 0.259899 8.3:+40112775 MS.gene34802:CDS
AAAATTGTCATTGGCCGATT+TGG 0.299413 8.3:-40112696 None:intergenic
CAACTTAAATTTGACGTTGA+TGG 0.335217 8.3:+40113570 MS.gene34802:CDS
CTCGCCGGTGGCGCCACCTC+TGG 0.341008 8.3:+40112051 MS.gene34802:CDS
TGATGTAAACGGCTAAATTC+AGG 0.379198 8.3:-40113070 None:intergenic
GGAAGGTGGAATTAAAGCTT+TGG 0.380976 8.3:-40112657 None:intergenic
TGCATCAGATTGGGCATCAT+TGG 0.387142 8.3:+40112758 MS.gene34802:CDS
CTCGGATGAGGTTGAGAATA+GGG 0.388430 8.3:-40112847 None:intergenic
GACGTTGATGGGTCTCATTA+AGG 0.393555 8.3:+40113582 MS.gene34802:CDS
CTGACTTATGTTCACATAGA+AGG 0.406124 8.3:+40113384 MS.gene34802:CDS
AACTTAAATTTGACGTTGAT+GGG 0.410278 8.3:+40113571 MS.gene34802:CDS
GTAAAGGAGGTTGGAGCAAT+TGG 0.413248 8.3:-40112351 None:intergenic
TATGAATGTCGCGGAATTTG+CGG 0.415114 8.3:-40112166 None:intergenic
TAGATTCACATTCGTTCAAA+AGG 0.416585 8.3:+40112111 MS.gene34802:CDS
AAATAATGAATGCATCAGAT+TGG 0.421459 8.3:+40112748 MS.gene34802:CDS
GAGATAGAGTTGTTGAAGGT+GGG 0.425273 8.3:-40112282 None:intergenic
ACTTGGGACTGCCAAATAGA+AGG 0.427636 8.3:-40112586 None:intergenic
CGATTTGGAGCTCTCGGAGC+TGG 0.442706 8.3:-40112681 None:intergenic
CCTTCCAGAGGTGGCGCCAC+CGG 0.463852 8.3:-40112055 None:intergenic
AATAATGAATGCATCAGATT+GGG 0.466409 8.3:+40112749 MS.gene34802:CDS
AGTCCAAGTAAAATTGTCAT+TGG 0.468154 8.3:-40112705 None:intergenic
CATCATAGCGACGGAGGTTG+TGG 0.470605 8.3:-40112021 None:intergenic
AACCCGCAAAACTTCCAACT+TGG 0.473471 8.3:-40112603 None:intergenic
CGCGGAATTTGCGGAGGGCG+AGG 0.475468 8.3:-40112157 None:intergenic
GCTCCAACCTCCTTTACCTC+AGG 0.478577 8.3:+40112357 MS.gene34802:CDS
TATACATCATAGCAGTCAGC+TGG 0.478695 8.3:-40112902 None:intergenic
TCTCGGATGAGGTTGAGAAT+AGG 0.481952 8.3:-40112848 None:intergenic
GAAGGTGGAATTAAAGCTTT+GGG 0.484659 8.3:-40112656 None:intergenic
GCAATTATAACCTGAGGTAA+AGG 0.484740 8.3:-40112367 None:intergenic
CATCAACGTCAAATTTAAGT+TGG 0.486323 8.3:-40113569 None:intergenic
TGTAGGTTCTTATAGTATAG+AGG 0.487842 8.3:+40112481 MS.gene34802:CDS
CTCAACCTCATCCGAGATGA+TGG 0.488519 8.3:+40112854 MS.gene34802:CDS
TACTCGGCTTTAATCGATCC+GGG 0.489291 8.3:+40112201 MS.gene34802:CDS
ACGTGGATCCTTGCTGCCAT+TGG 0.491503 8.3:+40112257 MS.gene34802:CDS
ATGTTTCGACTCAAATCTGC+TGG 0.494251 8.3:+40113111 MS.gene34802:CDS
CATAAGTTCCATCTCATACT+CGG 0.496407 8.3:+40112185 MS.gene34802:CDS
CTTCTATGTGAACATAAGTC+AGG 0.496958 8.3:-40113383 None:intergenic
ATACTCGGCTTTAATCGATC+CGG 0.499361 8.3:+40112200 MS.gene34802:CDS
GCCATTGAGAACATGGCAGC+TGG 0.499987 8.3:+40113326 MS.gene34802:CDS
ATTGGCAACTTTCACAGTAT+GGG 0.502787 8.3:+40112776 MS.gene34802:CDS
TGAACTTCAGATGATGTAAA+CGG 0.503521 8.3:-40113081 None:intergenic
GACAAATTCACTTTCAGTCA+AGG 0.506419 8.3:-40112822 None:intergenic
CAATTGGTCAAGAGGGAAGG+AGG 0.511905 8.3:-40112335 None:intergenic
TGCCATGTTCTCAATGGCAG+TGG 0.512316 8.3:-40113321 None:intergenic
TTCTGCCATCATCTCGGATG+AGG 0.513011 8.3:-40112859 None:intergenic
ATTGGCCGATTTGGAGCTCT+CGG 0.514913 8.3:-40112687 None:intergenic
TTGTTGAAGGTGGGGTCCAA+TGG 0.515637 8.3:-40112273 None:intergenic
TCCAGCTGCCATGTTCTCAA+TGG 0.515812 8.3:-40113327 None:intergenic
GTTCACATAGAAGGATATCA+AGG 0.522561 8.3:+40113393 MS.gene34802:CDS
TAACCTGAGGTAAAGGAGGT+TGG 0.524882 8.3:-40112360 None:intergenic
TGTAGAGATAGAGTTGTTGA+AGG 0.526137 8.3:-40112286 None:intergenic
CAGCTCCGAGAGCTCCAAAT+CGG 0.527982 8.3:+40112682 MS.gene34802:CDS
GAGCTCTCGGAGCTGGAGGA+AGG 0.528347 8.3:-40112674 None:intergenic
AGAGATAGAGTTGTTGAAGG+TGG 0.529187 8.3:-40112283 None:intergenic
AAGATTAACGAATATATCAA+AGG 0.534259 8.3:+40113533 MS.gene34802:CDS
TTGGCAACTTTCACAGTATG+GGG 0.536652 8.3:+40112777 MS.gene34802:CDS
TGATAGTTCAATAGACAAAA+CGG 0.539124 8.3:+40113491 MS.gene34802:CDS
ATGAAAGTTGTCATAAATCC+CGG 0.539720 8.3:-40112219 None:intergenic
GGAGGTTGTGGTAGGGAGGA+AGG 0.546045 8.3:-40112009 None:intergenic
CCGGTGGCGCCACCTCTGGA+AGG 0.547084 8.3:+40112055 MS.gene34802:CDS
AGATTAACGAATATATCAAA+GGG 0.548268 8.3:+40113534 MS.gene34802:CDS
TCTATTTGGCAGTCCCAAGT+TGG 0.550189 8.3:+40112589 MS.gene34802:CDS
AGCAGATTTGAGTCGAAACA+TGG 0.553943 8.3:-40113109 None:intergenic
TGTGGCATTGCTCAAGAACA+TGG 0.554101 8.3:-40113135 None:intergenic
CTCTCGGAGCTGGAGGAAGG+TGG 0.556854 8.3:-40112671 None:intergenic
AACCACTGCCATTGAGAACA+TGG 0.558537 8.3:+40113319 MS.gene34802:CDS
ATATGACACAGGATCGAGTC+CGG 0.559164 8.3:-40111981 None:intergenic
AACCTACTCGACGATGGCAA+CGG 0.559988 8.3:+40111893 None:intergenic
TTCAGGATAATTTAGAACAC+GGG 0.564152 8.3:-40113053 None:intergenic
AATTGGTCAAGAGGGAAGGA+GGG 0.570036 8.3:-40112334 None:intergenic
ACCCGCAAAACTTCCAACTT+GGG 0.570628 8.3:-40112602 None:intergenic
TTCAATAGACAAAACGGACA+TGG 0.570795 8.3:+40113497 MS.gene34802:CDS
GGACAGATTGAGCAGTCTAC+CGG 0.570908 8.3:+40111962 MS.gene34802:CDS
ATAGCGACGGAGGTTGTGGT+AGG 0.574834 8.3:-40112017 None:intergenic
AGATAGAGTTGTTGAAGGTG+GGG 0.580102 8.3:-40112281 None:intergenic
GATTAAAGCCGAGTATGAGA+TGG 0.586475 8.3:-40112193 None:intergenic
AGAGACAGCACTTTGAGCAA+TGG 0.588422 8.3:-40112527 None:intergenic
ATTCAGTTCTGCCATCATCT+CGG 0.593799 8.3:-40112865 None:intergenic
GAGCAATTGGTCAAGAGGGA+AGG 0.596231 8.3:-40112338 None:intergenic
GGTTGGAGCAATTGGTCAAG+AGG 0.598188 8.3:-40112343 None:intergenic
ATCTCGCTTGTATTCGTACG+TGG 0.599453 8.3:+40112240 MS.gene34802:CDS
GTTGCGTCATTCAACATCAA+AGG 0.603041 8.3:+40112931 MS.gene34802:CDS
CTATGTGAACATAAGTCAGG+TGG 0.604289 8.3:-40113380 None:intergenic
GAATGTCGCGGAATTTGCGG+AGG 0.613849 8.3:-40112163 None:intergenic
GATGGCAACGGAGTCAAGCA+TGG 0.614265 8.3:+40111905 None:intergenic
TAGCGACGGAGGTTGTGGTA+GGG 0.614525 8.3:-40112016 None:intergenic
TAATTTAGAACACGGGCATG+AGG 0.618358 8.3:-40113046 None:intergenic
GTTGGAGCAATTGGTCAAGA+GGG 0.618704 8.3:-40112342 None:intergenic
CGACGGAGGTTGTGGTAGGG+AGG 0.621858 8.3:-40112013 None:intergenic
GGTGGGGTCCAATGGCAGCA+AGG 0.624675 8.3:-40112265 None:intergenic
ATTATAACCTGAGGTAAAGG+AGG 0.629004 8.3:-40112364 None:intergenic
ATTCAGGATAATTTAGAACA+CGG 0.629666 8.3:-40113054 None:intergenic
TGATGAGAGTCTCTCGCCGG+TGG 0.630142 8.3:+40112039 MS.gene34802:CDS
TTTGAGCAATGGAAAATGAA+CGG 0.634422 8.3:-40112516 None:intergenic
TTTGGAGCTCTCGGAGCTGG+AGG 0.636682 8.3:-40112678 None:intergenic
TCTGCAGATCCTTCCAGAGG+TGG 0.638103 8.3:-40112064 None:intergenic
TATGTGAACATAAGTCAGGT+GGG 0.640734 8.3:-40113379 None:intergenic
CTATGATGAGAGTCTCTCGC+CGG 0.640993 8.3:+40112036 MS.gene34802:CDS
ATACATCATAGCAGTCAGCT+GGG 0.647711 8.3:-40112901 None:intergenic
TCTCATAATTCAGAGCAACA+AGG 0.652411 8.3:+40113168 MS.gene34802:CDS
GGAAGGACATGATATGACAC+AGG 0.667460 8.3:-40111992 None:intergenic
AGAGACTCTCATCATAGCGA+CGG 0.669339 8.3:-40112030 None:intergenic
TGGCAACTTTCACAGTATGG+GGG 0.681502 8.3:+40112778 MS.gene34802:CDS
AATGTCGCGGAATTTGCGGA+GGG 0.681823 8.3:-40112162 None:intergenic
GATGGAACTTATGAATGTCG+CGG 0.686296 8.3:-40112175 None:intergenic
GACTCTCATCATAGCGACGG+AGG 0.689212 8.3:-40112027 None:intergenic
TTATGAGAACTTGAAGCATG+TGG 0.695487 8.3:-40113153 None:intergenic
TGCAGAAGAAGACAACGTCA+CGG 0.699654 8.3:+40111941 MS.gene34802:CDS
ACTGCTATGATGTATATCTG+CGG 0.701238 8.3:+40112909 MS.gene34802:CDS
ATATCTGCAGATCCTTCCAG+AGG 0.715901 8.3:-40112067 None:intergenic
CATCATAGCAGTCAGCTGGG+AGG 0.736987 8.3:-40112898 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATTAACGAATATATCAA+AGG + chr8.3:40113533-40113552 MS.gene34802:CDS 20.0%
!! ACATACATACATGTATATAT+AGG - chr8.3:40113236-40113255 None:intergenic 20.0%
!! AGATTAACGAATATATCAAA+GGG + chr8.3:40113534-40113553 MS.gene34802:CDS 20.0%
!! AGTTTAATACAAACAATCTT+TGG + chr8.3:40113206-40113225 MS.gene34802:intron 20.0%
!! ATGGTTTATATGTATATCTA+CGG + chr8.3:40112969-40112988 MS.gene34802:intron 20.0%
!! GTCATTACTCAAAAATATAT+TGG - chr8.3:40113272-40113291 None:intergenic 20.0%
!!! TATATTTTTGAGTAATGACA+TGG + chr8.3:40113273-40113292 MS.gene34802:intron 20.0%
! AAATAATGAATGCATCAGAT+TGG + chr8.3:40112748-40112767 MS.gene34802:CDS 25.0%
! AACTTAAATTTGACGTTGAT+GGG + chr8.3:40113571-40113590 MS.gene34802:CDS 25.0%
! AATAATGAATGCATCAGATT+GGG + chr8.3:40112749-40112768 MS.gene34802:CDS 25.0%
! GAAATAGTTTACCTTCTATT+TGG + chr8.3:40112575-40112594 MS.gene34802:CDS 25.0%
! TAAAAAGCAATTATAACCTG+AGG - chr8.3:40112376-40112395 None:intergenic 25.0%
! TGATAGTTCAATAGACAAAA+CGG + chr8.3:40113491-40113510 MS.gene34802:CDS 25.0%
! TTATATGTATATCTACGGTT+TGG + chr8.3:40112974-40112993 MS.gene34802:intron 25.0%
!! ATTCAGGATAATTTAGAACA+CGG - chr8.3:40113057-40113076 None:intergenic 25.0%
!!! AAGGTGATGTTTTATTCTTA+TGG + chr8.3:40112950-40112969 MS.gene34802:intron 25.0%
!!! CATTTGCTGAATATATTTTG+CGG + chr8.3:40113433-40113452 MS.gene34802:CDS 25.0%
AGTCCAAGTAAAATTGTCAT+TGG - chr8.3:40112708-40112727 None:intergenic 30.0%
ATGAAAGTTGTCATAAATCC+CGG - chr8.3:40112222-40112241 None:intergenic 30.0%
CAACTTAAATTTGACGTTGA+TGG + chr8.3:40113570-40113589 MS.gene34802:CDS 30.0%
CATCAACGTCAAATTTAAGT+TGG - chr8.3:40113572-40113591 None:intergenic 30.0%
GAAATGTTAGAAGTGAGTTT+GGG - chr8.3:40112401-40112420 None:intergenic 30.0%
TAGATTCACATTCGTTCAAA+AGG + chr8.3:40112111-40112130 MS.gene34802:CDS 30.0%
TGAAATGTTAGAAGTGAGTT+TGG - chr8.3:40112402-40112421 None:intergenic 30.0%
TGAACTTCAGATGATGTAAA+CGG - chr8.3:40113084-40113103 None:intergenic 30.0%
TGTAGGTTCTTATAGTATAG+AGG + chr8.3:40112481-40112500 MS.gene34802:CDS 30.0%
TTTGAGCAATGGAAAATGAA+CGG - chr8.3:40112519-40112538 None:intergenic 30.0%
! TTCAGGATAATTTAGAACAC+GGG - chr8.3:40113056-40113075 None:intergenic 30.0%
! TTTTAGACTCACAATTTCTG+CGG - chr8.3:40112555-40112574 None:intergenic 30.0%
!! TCTTTTGAAAACAGTCTTGT+AGG + chr8.3:40112464-40112483 MS.gene34802:intron 30.0%
AAAATTGTCATTGGCCGATT+TGG - chr8.3:40112699-40112718 None:intergenic 35.0%
ACTGCTATGATGTATATCTG+CGG + chr8.3:40112909-40112928 MS.gene34802:CDS 35.0%
ATTATAACCTGAGGTAAAGG+AGG - chr8.3:40112367-40112386 None:intergenic 35.0%
ATTGGCAACTTTCACAGTAT+GGG + chr8.3:40112776-40112795 MS.gene34802:CDS 35.0%
CATAAGTTCCATCTCATACT+CGG + chr8.3:40112185-40112204 MS.gene34802:CDS 35.0%
CTGACTTATGTTCACATAGA+AGG + chr8.3:40113384-40113403 MS.gene34802:CDS 35.0%
CTTCTATGTGAACATAAGTC+AGG - chr8.3:40113386-40113405 None:intergenic 35.0%
GAAGGTGGAATTAAAGCTTT+GGG - chr8.3:40112659-40112678 None:intergenic 35.0%
GACAAATTCACTTTCAGTCA+AGG - chr8.3:40112825-40112844 None:intergenic 35.0%
GCAATTATAACCTGAGGTAA+AGG - chr8.3:40112370-40112389 None:intergenic 35.0%
GTTCACATAGAAGGATATCA+AGG + chr8.3:40113393-40113412 MS.gene34802:CDS 35.0%
TATGTGAACATAAGTCAGGT+GGG - chr8.3:40113382-40113401 None:intergenic 35.0%
TCTCATAATTCAGAGCAACA+AGG + chr8.3:40113168-40113187 MS.gene34802:CDS 35.0%
TGATGTAAACGGCTAAATTC+AGG - chr8.3:40113073-40113092 None:intergenic 35.0%
TGTAGAGATAGAGTTGTTGA+AGG - chr8.3:40112289-40112308 None:intergenic 35.0%
TTATGAGAACTTGAAGCATG+TGG - chr8.3:40113156-40113175 None:intergenic 35.0%
TTCAATAGACAAAACGGACA+TGG + chr8.3:40113497-40113516 MS.gene34802:CDS 35.0%
! GCTGAATATATTTTGCGGAA+TGG + chr8.3:40113438-40113457 MS.gene34802:CDS 35.0%
AATGACATGGTTTCCCTTGT+TGG + chr8.3:40113286-40113305 MS.gene34802:intron 40.0%
AGAGATAGAGTTGTTGAAGG+TGG - chr8.3:40112286-40112305 None:intergenic 40.0%
AGATAGAGTTGTTGAAGGTG+GGG - chr8.3:40112284-40112303 None:intergenic 40.0%
AGCAGATTTGAGTCGAAACA+TGG - chr8.3:40113112-40113131 None:intergenic 40.0%
ATACATCATAGCAGTCAGCT+GGG - chr8.3:40112904-40112923 None:intergenic 40.0%
ATGTTTCGACTCAAATCTGC+TGG + chr8.3:40113111-40113130 MS.gene34802:CDS 40.0%
ATTCAGTTCTGCCATCATCT+CGG - chr8.3:40112868-40112887 None:intergenic 40.0%
CATTGGCAACTTTCACAGTA+TGG + chr8.3:40112775-40112794 MS.gene34802:CDS 40.0%
CTATGTGAACATAAGTCAGG+TGG - chr8.3:40113383-40113402 None:intergenic 40.0%
GAGATAGAGTTGTTGAAGGT+GGG - chr8.3:40112285-40112304 None:intergenic 40.0%
GATGGAACTTATGAATGTCG+CGG - chr8.3:40112178-40112197 None:intergenic 40.0%
GATTAAAGCCGAGTATGAGA+TGG - chr8.3:40112196-40112215 None:intergenic 40.0%
GGAAGGTGGAATTAAAGCTT+TGG - chr8.3:40112660-40112679 None:intergenic 40.0%
GTTGCGTCATTCAACATCAA+AGG + chr8.3:40112931-40112950 MS.gene34802:CDS 40.0%
TATACATCATAGCAGTCAGC+TGG - chr8.3:40112905-40112924 None:intergenic 40.0%
TATGAATGTCGCGGAATTTG+CGG - chr8.3:40112169-40112188 None:intergenic 40.0%
TTGGCAACTTTCACAGTATG+GGG + chr8.3:40112777-40112796 MS.gene34802:CDS 40.0%
! ATACTCGGCTTTAATCGATC+CGG + chr8.3:40112200-40112219 MS.gene34802:CDS 40.0%
! CGGCCAATGACAATTTTACT+TGG + chr8.3:40112702-40112721 MS.gene34802:CDS 40.0%
! TAATTTAGAACACGGGCATG+AGG - chr8.3:40113049-40113068 None:intergenic 40.0%
AACCACTGCCATTGAGAACA+TGG + chr8.3:40113319-40113338 MS.gene34802:CDS 45.0%
AACCCGCAAAACTTCCAACT+TGG - chr8.3:40112606-40112625 None:intergenic 45.0%
AATTGGTCAAGAGGGAAGGA+GGG - chr8.3:40112337-40112356 None:intergenic 45.0%
ACCCGCAAAACTTCCAACTT+GGG - chr8.3:40112605-40112624 None:intergenic 45.0%
ACTTGGGACTGCCAAATAGA+AGG - chr8.3:40112589-40112608 None:intergenic 45.0%
AGAGACAGCACTTTGAGCAA+TGG - chr8.3:40112530-40112549 None:intergenic 45.0%
AGAGACTCTCATCATAGCGA+CGG - chr8.3:40112033-40112052 None:intergenic 45.0%
ATATCTGCAGATCCTTCCAG+AGG - chr8.3:40112070-40112089 None:intergenic 45.0%
ATATGACACAGGATCGAGTC+CGG - chr8.3:40111984-40112003 None:intergenic 45.0%
ATCTCGCTTGTATTCGTACG+TGG + chr8.3:40112240-40112259 MS.gene34802:CDS 45.0%
CTCGGATGAGGTTGAGAATA+GGG - chr8.3:40112850-40112869 None:intergenic 45.0%
GGAAGGACATGATATGACAC+AGG - chr8.3:40111995-40112014 None:intergenic 45.0%
GTAAAGGAGGTTGGAGCAAT+TGG - chr8.3:40112354-40112373 None:intergenic 45.0%
GTTGGAGCAATTGGTCAAGA+GGG - chr8.3:40112345-40112364 None:intergenic 45.0%
TAACCTGAGGTAAAGGAGGT+TGG - chr8.3:40112363-40112382 None:intergenic 45.0%
TCTATTTGGCAGTCCCAAGT+TGG + chr8.3:40112589-40112608 MS.gene34802:CDS 45.0%
TCTCGGATGAGGTTGAGAAT+AGG - chr8.3:40112851-40112870 None:intergenic 45.0%
TGCAGAAGAAGACAACGTCA+CGG + chr8.3:40111941-40111960 MS.gene34802:CDS 45.0%
TGCATCAGATTGGGCATCAT+TGG + chr8.3:40112758-40112777 MS.gene34802:CDS 45.0%
TGGCAACTTTCACAGTATGG+GGG + chr8.3:40112778-40112797 MS.gene34802:CDS 45.0%
TGTGGCATTGCTCAAGAACA+TGG - chr8.3:40113138-40113157 None:intergenic 45.0%
TTGTTATCGTGTACCTGCAG+TGG + chr8.3:40113020-40113039 MS.gene34802:intron 45.0%
! TACTCGGCTTTAATCGATCC+GGG + chr8.3:40112201-40112220 MS.gene34802:CDS 45.0%
!! GTCCCAAGTTGGAAGTTTTG+CGG + chr8.3:40112600-40112619 MS.gene34802:CDS 45.0%
!! TCCCAAGTTGGAAGTTTTGC+GGG + chr8.3:40112601-40112620 MS.gene34802:CDS 45.0%
AATGTCGCGGAATTTGCGGA+GGG - chr8.3:40112165-40112184 None:intergenic 50.0%
CAATTGGTCAAGAGGGAAGG+AGG - chr8.3:40112338-40112357 None:intergenic 50.0%
CTATGATGAGAGTCTCTCGC+CGG + chr8.3:40112036-40112055 MS.gene34802:CDS 50.0%
CTCAACCTCATCCGAGATGA+TGG + chr8.3:40112854-40112873 MS.gene34802:CDS 50.0%
GACATGGTTTCCCTTGTTGG+TGG + chr8.3:40113289-40113308 MS.gene34802:intron 50.0%
GAGCAATTGGTCAAGAGGGA+AGG - chr8.3:40112341-40112360 None:intergenic 50.0%
GGACAGATTGAGCAGTCTAC+CGG + chr8.3:40111962-40111981 MS.gene34802:CDS 50.0%
GGTTGGAGCAATTGGTCAAG+AGG - chr8.3:40112346-40112365 None:intergenic 50.0%
GTTCCTCCTACCACCAACAA+GGG - chr8.3:40113302-40113321 None:intergenic 50.0%
TCCAGCTGCCATGTTCTCAA+TGG - chr8.3:40113330-40113349 None:intergenic 50.0%
TGCCATGTTCTCAATGGCAG+TGG - chr8.3:40113324-40113343 None:intergenic 50.0%
TTCTGCCATCATCTCGGATG+AGG - chr8.3:40112862-40112881 None:intergenic 50.0%
TTGTTGAAGGTGGGGTCCAA+TGG - chr8.3:40112276-40112295 None:intergenic 50.0%
!! ATTGGCCGATTTGGAGCTCT+CGG - chr8.3:40112690-40112709 None:intergenic 50.0%
!! TGGTTTCCCTTGTTGGTGGT+AGG + chr8.3:40113293-40113312 MS.gene34802:intron 50.0%
!! TTTCCCTTGTTGGTGGTAGG+AGG + chr8.3:40113296-40113315 MS.gene34802:intron 50.0%
ACGTGGATCCTTGCTGCCAT+TGG + chr8.3:40112257-40112276 MS.gene34802:CDS 55.0%
ATAGCGACGGAGGTTGTGGT+AGG - chr8.3:40112020-40112039 None:intergenic 55.0%
CAGCTCCGAGAGCTCCAAAT+CGG + chr8.3:40112682-40112701 MS.gene34802:CDS 55.0%
CATCATAGCAGTCAGCTGGG+AGG - chr8.3:40112901-40112920 None:intergenic 55.0%
CATCATAGCGACGGAGGTTG+TGG - chr8.3:40112024-40112043 None:intergenic 55.0%
GAATGTCGCGGAATTTGCGG+AGG - chr8.3:40112166-40112185 None:intergenic 55.0%
GACTCTCATCATAGCGACGG+AGG - chr8.3:40112030-40112049 None:intergenic 55.0%
GCCATTGAGAACATGGCAGC+TGG + chr8.3:40113326-40113345 MS.gene34802:CDS 55.0%
GCTCCAACCTCCTTTACCTC+AGG + chr8.3:40112357-40112376 MS.gene34802:CDS 55.0%
GGTTCCTCCTACCACCAACA+AGG - chr8.3:40113303-40113322 None:intergenic 55.0%
TAGCGACGGAGGTTGTGGTA+GGG - chr8.3:40112019-40112038 None:intergenic 55.0%
TCTGCAGATCCTTCCAGAGG+TGG - chr8.3:40112067-40112086 None:intergenic 55.0%
GGAGGTTGTGGTAGGGAGGA+AGG - chr8.3:40112012-40112031 None:intergenic 60.0%
TGATGAGAGTCTCTCGCCGG+TGG + chr8.3:40112039-40112058 MS.gene34802:CDS 60.0%
! CGATTTGGAGCTCTCGGAGC+TGG - chr8.3:40112684-40112703 None:intergenic 60.0%
! TTTGGAGCTCTCGGAGCTGG+AGG - chr8.3:40112681-40112700 None:intergenic 60.0%
CGACGGAGGTTGTGGTAGGG+AGG - chr8.3:40112016-40112035 None:intergenic 65.0%
CTCTCGGAGCTGGAGGAAGG+TGG - chr8.3:40112674-40112693 None:intergenic 65.0%
GAGCTCTCGGAGCTGGAGGA+AGG - chr8.3:40112677-40112696 None:intergenic 65.0%
GGTGGGGTCCAATGGCAGCA+AGG - chr8.3:40112268-40112287 None:intergenic 65.0%
CCTTCCAGAGGTGGCGCCAC+CGG - chr8.3:40112058-40112077 None:intergenic 70.0%
CGCGGAATTTGCGGAGGGCG+AGG - chr8.3:40112160-40112179 None:intergenic 70.0%
GGGCATGAGGTCGCCACTGC+AGG - chr8.3:40113036-40113055 None:intergenic 70.0%
CCGGTGGCGCCACCTCTGGA+AGG + chr8.3:40112055-40112074 MS.gene34802:CDS 75.0%
! CTCGCCGGTGGCGCCACCTC+TGG + chr8.3:40112051-40112070 MS.gene34802:CDS 80.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 40111906 40113602 40111906 ID=MS.gene34802
chr8.3 mRNA 40111906 40113602 40111906 ID=MS.gene34802.t1;Parent=MS.gene34802
chr8.3 exon 40111906 40112378 40111906 ID=MS.gene34802.t1.exon1;Parent=MS.gene34802.t1
chr8.3 CDS 40111906 40112378 40111906 ID=cds.MS.gene34802.t1;Parent=MS.gene34802.t1
chr8.3 exon 40112478 40112952 40112478 ID=MS.gene34802.t1.exon2;Parent=MS.gene34802.t1
chr8.3 CDS 40112478 40112952 40112478 ID=cds.MS.gene34802.t1;Parent=MS.gene34802.t1
chr8.3 exon 40113040 40113189 40113040 ID=MS.gene34802.t1.exon3;Parent=MS.gene34802.t1
chr8.3 CDS 40113040 40113189 40113040 ID=cds.MS.gene34802.t1;Parent=MS.gene34802.t1
chr8.3 exon 40113315 40113602 40113315 ID=MS.gene34802.t1.exon4;Parent=MS.gene34802.t1
chr8.3 CDS 40113315 40113602 40113315 ID=cds.MS.gene34802.t1;Parent=MS.gene34802.t1
Gene Sequence

>MS.gene34802

ATGGCAACGGAGTCAAGCATGGAACGATCGCTTGTTGCAGAAGAAGACAACGTCACGGACAGATTGAGCAGTCTACCGGACTCGATCCTGTGTCATATCATGTCCTTCCTCCCTACCACAACCTCCGTCGCTATGATGAGAGTCTCTCGCCGGTGGCGCCACCTCTGGAAGGATCTGCAGATATTCAACTTCTGCAGTCGCAATCTAGATTCACATTCGTTCAAAAGGTTTTTATTATTCGTGAACGCTGTCCTCGCCCTCCGCAAATTCCGCGACATTCATAAGTTCCATCTCATACTCGGCTTTAATCGATCCGGGATTTATGACAACTTTCATCTCGCTTGTATTCGTACGTGGATCCTTGCTGCCATTGGACCCCACCTTCAACAACTCTATCTCTACATTGTCAATGCTGCTGTTGTAGTTCTCCCTCCTTCCCTCTTGACCAATTGCTCCAACCTCCTTTACCTCAGGTTATAATTGCTTTTTATGCCCAAACTCACTTCTAACATTTCATTTCTTGTATTTTTACTTTTTTTTTTCTTTCTAACATGTTTGTCTTTTGAAAACAGTCTTGTAGGTTCTTATAGTATAGAGGTAAAACAGTCTTCCGTTCATTTTCCATTGCTCAAAGTGCTGTCTCTCGCCGCAGAAATTGTGAGTCTAAAAGAAATAGTTTACCTTCTATTTGGCAGTCCCAAGTTGGAAGTTTTGCGGGTTTACTTTTACTTTATCTCTGAAGAACTTGTGCCCAAAGCTTTAATTCCACCTTCCTCCAGCTCCGAGAGCTCCAAATCGGCCAATGACAATTTTACTTGGACTTACTTTAAAATAGTTAACACAAATAATGAATGCATCAGATTGGGCATCATTGGCAACTTTCACAGTATGGGGGAAGCATTTCTTGATGTCTTTTCCTTGACTGAAAGTGAATTTGTCGACCCTATTCTCAACCTCATCCGAGATGATGGCAGAACTGAATTTTATAAAAACCTCCCAGCTGACTGCTATGATGTATATCTGCGGTTGCGTCATTCAACATCAAAGGTGATGTTTTATTCTTATGGTTTATATGTATATCTACGGTTTGGTACTAATAGTGCGTCATCACTTGTTGTTATCGTGTACCTGCAGTGGCGACCTCATGCCCGTGTTCTAAATTATCCTGAATTTAGCCGTTTACATCATCTGAAGTTCATTCTTCCATGTTTCGACTCAAATCTGCTGGTCCATGTTCTTGAGCAATGCCACATGCTTCAAGTTCTCATAATTCAGAGCAACAAGGTTCGTTGACTTCTCAAGTTTAATACAAACAATCTTTGGTCTGCCTATATATACATGTATGTATGTATCTATCTGTACACCAATATATTTTTGAGTAATGACATGGTTTCCCTTGTTGGTGGTAGGAGGAACCACTGCCATTGAGAACATGGCAGCTGGAGTCAACAACAGTTTTTGAATGTCTAAAATCCCACCTGACTTATGTTCACATAGAAGGATATCAAGGACTTGAAGATGAGTTCACATTTGCTGAATATATTTTGCGGAATGGACTTCTTTTGAAGACGATGCTTATTTTCGTTGATAGTTCAATAGACAAAACGGACATGGATTGTTCTCTCAAAAGATTAACGAATATATCAAAGGGCTCTGTTGCTTGCCAACTTAAATTTGACGTTGATGGGTCTCATTAA
Protein sequence

>MS.gene34802.t1

MATESSMERSLVAEEDNVTDRLSSLPDSILCHIMSFLPTTTSVAMMRVSRRWRHLWKDLQIFNFCSRNLDSHSFKRFLLFVNAVLALRKFRDIHKFHLILGFNRSGIYDNFHLACIRTWILAAIGPHLQQLYLYIVNAAVVVLPPSLLTNCSNLLYLSLVGSYSIEVKQSSVHFPLLKVLSLAAEIVSLKEIVYLLFGSPKLEVLRVYFYFISEELVPKALIPPSSSSESSKSANDNFTWTYFKIVNTNNECIRLGIIGNFHSMGEAFLDVFSLTESEFVDPILNLIRDDGRTEFYKNLPADCYDVYLRLRHSTSKWRPHARVLNYPEFSRLHHLKFILPCFDSNLLVHVLEQCHMLQVLIIQSNKEEPLPLRTWQLESTTVFECLKSHLTYVHIEGYQGLEDEFTFAEYILRNGLLLKTMLIFVDSSIDKTDMDCSLKRLTNISKGSVACQLKFDVDGSH