AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25386

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25386.t1 MTR_8g062300 55.870 494 165 10 1 453 1 482 5.04e-168 481
MS.gene25386.t1 MTR_8g062080 56.156 463 180 9 1 453 1 450 3.83e-163 468
MS.gene25386.t1 MTR_8g070950 57.447 470 150 11 1 452 1 438 7.00e-162 464
MS.gene25386.t1 MTR_8g062310 53.675 449 182 7 1 448 5 428 2.68e-153 442
MS.gene25386.t1 MTR_8g062010 55.481 447 161 10 23 452 9 434 1.88e-151 437
MS.gene25386.t1 MTR_8g469020 50.426 470 174 13 22 452 6 455 6.65e-138 404
MS.gene25386.t1 MTR_8g058965 48.240 483 186 12 10 453 4 461 3.09e-130 384
MS.gene25386.t1 MTR_4g050250 47.630 443 160 13 1 394 1 420 1.06e-109 331
MS.gene25386.t1 MTR_8g467790 46.005 413 143 12 23 408 148 507 1.06e-100 310
MS.gene25386.t1 MTR_8g468810 37.732 485 243 15 1 448 1 463 4.40e-83 263
MS.gene25386.t1 MTR_2g083840 38.780 459 228 12 22 448 20 457 5.24e-78 250
MS.gene25386.t1 MTR_8g062270 37.581 463 232 14 20 447 13 453 6.33e-77 247
MS.gene25386.t1 MTR_2g086020 83.448 145 24 0 308 452 175 319 2.03e-74 236
MS.gene25386.t1 MTR_2g086020 59.091 154 55 2 49 198 1 150 1.89e-48 169
MS.gene25386.t1 MTR_8g058970 36.400 500 240 18 3 448 2 477 2.19e-73 239
MS.gene25386.t1 MTR_4g032730 35.845 491 237 15 9 447 2 466 8.63e-72 234
MS.gene25386.t1 MTR_8g014260 37.740 469 244 20 9 448 2 451 2.12e-69 228
MS.gene25386.t1 MTR_8g467800 37.421 473 237 17 8 448 8 453 4.58e-68 224
MS.gene25386.t1 MTR_8g468840 35.760 467 227 15 21 445 12 447 9.64e-65 215
MS.gene25386.t1 MTR_8g468810 37.913 393 187 14 1 358 1 371 2.12e-61 204
MS.gene25386.t1 MTR_5g092960 33.404 473 261 12 11 447 7 461 8.18e-61 205
MS.gene25386.t1 MTR_8g468760 36.009 461 228 17 10 447 2 418 1.49e-60 203
MS.gene25386.t1 MTR_8g445780 35.529 425 215 16 1 401 1 390 1.13e-53 184
MS.gene25386.t1 MTR_8g468810 40.193 311 144 11 1 281 1 299 7.66e-53 180
MS.gene25386.t1 MTR_8g468810 40.193 311 144 11 1 281 1 299 1.13e-52 180
MS.gene25386.t1 MTR_8g468810 40.193 311 144 11 1 281 1 299 1.95e-52 180
MS.gene25386.t1 MTR_5g092960 32.292 384 212 10 7 358 4 371 2.56e-38 144
MS.gene25386.t1 MTR_4g024710 35.216 301 160 11 166 448 64 347 3.83e-36 136
MS.gene25386.t1 MTR_8g467800 38.127 299 147 13 8 281 8 293 1.68e-33 129
MS.gene25386.t1 MTR_8g077460 44.966 149 78 4 301 448 230 375 9.26e-32 125
MS.gene25386.t1 MTR_8g077460 36.453 203 111 7 23 212 10 207 2.69e-22 98.6
MS.gene25386.t1 MTR_8g014210 40.816 196 100 6 254 448 51 231 8.66e-31 119
MS.gene25386.t1 MTR_7g098510 27.356 435 248 11 22 448 17 391 1.63e-28 116
MS.gene25386.t1 MTR_3g099800 67.901 81 25 1 360 439 19 99 2.30e-28 108
MS.gene25386.t1 MTR_8g014160 34.296 277 152 9 14 265 32 303 5.89e-28 115
MS.gene25386.t1 MTR_8g014160 51.613 93 41 2 356 448 337 425 4.74e-19 89.4
MS.gene25386.t1 MTR_1g041245 28.764 445 231 18 22 448 10 386 1.41e-25 108
MS.gene25386.t1 MTR_5g021550 44.079 152 71 7 17 156 13 162 6.56e-25 100
MS.gene25386.t1 MTR_5g008950 27.107 439 231 17 25 448 1 365 3.79e-24 103
MS.gene25386.t1 MTR_4g024745 32.414 290 149 11 166 431 18 284 5.55e-24 101
MS.gene25386.t1 MTR_3g107160 25.618 445 257 15 15 448 2 383 1.00e-20 94.0
MS.gene25386.t1 MTR_8g014290 36.601 153 77 5 23 157 10 160 1.17e-20 89.0
MS.gene25386.t1 MTR_7g098480 27.438 441 236 15 22 447 17 388 1.58e-20 94.4
MS.gene25386.t1 MTR_5g008920 25.858 437 249 15 23 448 9 381 3.94e-19 89.4
MS.gene25386.t1 MTR_7g026500 25.056 443 252 15 23 447 14 394 1.21e-18 87.8
MS.gene25386.t1 MTR_7g098570 25.571 438 250 14 22 447 19 392 1.97e-18 87.8
MS.gene25386.t1 MTR_7g033990 27.273 440 223 18 23 440 11 375 3.09e-18 87.0
MS.gene25386.t1 MTR_5g073420 27.670 412 211 19 23 420 11 349 5.25e-18 85.9
MS.gene25386.t1 MTR_3g107100 25.499 451 256 16 19 448 7 398 1.74e-17 84.7
MS.gene25386.t1 MTR_5g080710 27.253 455 267 20 23 447 33 453 2.54e-17 84.3
MS.gene25386.t1 MTR_2g007450 28.205 468 241 23 16 446 9 418 3.23e-17 84.0
MS.gene25386.t1 MTR_7g056447 31.739 230 133 9 23 248 9 218 2.56e-16 79.7
MS.gene25386.t1 MTR_7g055943 24.176 455 245 21 22 448 39 421 6.08e-16 80.1
MS.gene25386.t1 MTR_4g024720 39.231 130 56 7 8 126 10 127 7.61e-16 76.6
MS.gene25386.t1 MTR_7g034115 28.281 442 228 22 21 444 9 379 1.26e-15 78.6
MS.gene25386.t1 MTR_7g032380 33.333 192 111 7 23 208 11 191 7.82e-15 75.9
MS.gene25386.t1 MTR_7g115270 31.364 220 115 11 23 219 12 218 1.76e-14 75.5
MS.gene25386.t1 MTR_6g040270 24.368 435 251 19 25 446 10 379 2.84e-14 74.7
MS.gene25386.t1 MTR_3g036250 23.488 430 253 14 22 445 4 363 8.05e-14 73.2
MS.gene25386.t1 MTR_6g011460 26.222 450 250 20 15 448 38 421 8.57e-14 73.6
MS.gene25386.t1 MTR_3g082890 26.386 451 244 20 23 448 97 484 1.55e-13 72.8
MS.gene25386.t1 MTR_8g014230 45.122 82 41 2 357 438 18 95 2.37e-13 66.6
MS.gene25386.t1 MTR_5g045170 23.928 443 273 15 13 447 4 390 5.20e-13 70.9
MS.gene25386.t1 MTR_6g022360 24.249 433 260 14 19 447 39 407 1.73e-12 69.3
MS.gene25386.t1 MTR_5g083890 25.114 438 244 16 23 430 6 389 2.22e-12 69.3
MS.gene25386.t1 MTR_1g053025 25.792 442 252 21 23 445 2 386 4.68e-12 67.8
MS.gene25386.t1 MTR_2g007400 23.913 460 252 20 21 448 10 403 1.28e-11 66.6
MS.gene25386.t1 MTR_8g085430 32.512 203 107 10 22 208 10 198 3.73e-11 64.7
MS.gene25386.t1 MTR_3g105060 27.778 198 131 7 23 217 14 202 5.57e-11 64.3
MS.gene25386.t1 MTR_2g007500 31.188 202 95 8 22 208 13 185 8.66e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25386.t1 AT5G56420 26.682 446 278 18 23 448 6 422 5.54e-19 89.4
MS.gene25386.t1 AT5G56420 26.682 446 278 18 23 448 6 422 5.54e-19 89.4
MS.gene25386.t1 AT5G56420 26.682 446 278 18 23 448 6 422 5.54e-19 89.4
MS.gene25386.t1 AT1G32375 27.716 451 271 18 23 448 2 422 2.93e-18 87.0
MS.gene25386.t1 AT1G32375 27.716 451 271 18 23 448 2 422 2.93e-18 87.0
MS.gene25386.t1 AT1G78750 26.667 480 281 24 9 445 4 455 2.94e-18 87.4
MS.gene25386.t1 AT1G78750 26.667 480 281 24 9 445 4 455 2.94e-18 87.4
MS.gene25386.t1 AT5G53635 26.211 454 274 18 25 447 2 425 6.88e-17 82.8
MS.gene25386.t1 AT4G13965 26.000 450 281 17 16 445 3 420 1.62e-15 78.6
MS.gene25386.t1 AT1G13780 24.411 467 285 20 23 445 10 452 2.12e-14 75.5
MS.gene25386.t1 AT4G09920 32.474 194 114 7 23 210 2 184 5.05e-14 73.2
MS.gene25386.t1 AT4G09920 32.474 194 114 7 23 210 2 184 5.05e-14 73.2
MS.gene25386.t1 AT4G09920 32.474 194 114 7 23 210 2 184 5.43e-14 72.8
MS.gene25386.t1 AT4G09920 32.474 194 114 7 23 210 2 184 5.43e-14 72.8
MS.gene25386.t1 AT4G09920 32.474 194 114 7 23 210 2 184 5.43e-14 72.8
MS.gene25386.t1 AT3G59200 33.668 199 107 10 23 208 7 193 1.06e-13 72.8
MS.gene25386.t1 AT1G66320 25.887 479 274 24 7 445 17 454 1.10e-13 73.2
MS.gene25386.t1 AT1G78760 24.689 482 279 21 14 449 2 445 1.29e-13 73.2
MS.gene25386.t1 AT1G69630 26.124 467 279 19 11 440 6 443 1.65e-13 72.8
MS.gene25386.t1 AT3G59200 33.668 199 107 10 23 208 7 193 1.66e-13 72.8
MS.gene25386.t1 AT3G58940 30.986 213 107 9 23 210 2 199 2.78e-13 72.4
MS.gene25386.t1 AT3G58940 30.986 213 107 9 23 210 2 199 2.78e-13 72.4
MS.gene25386.t1 AT1G66290 24.740 481 272 26 9 445 16 450 2.89e-13 72.0
MS.gene25386.t1 AT1G51370 25.618 445 274 19 23 443 19 430 3.00e-13 71.6
MS.gene25386.t1 AT4G13985 23.848 499 283 22 1 445 1 456 7.17e-13 70.9
MS.gene25386.t1 AT5G56380 26.039 457 288 18 23 448 2 439 3.33e-12 68.6
MS.gene25386.t1 AT5G56380 26.039 457 288 18 23 448 2 439 3.33e-12 68.6
MS.gene25386.t1 AT1G13780 29.596 223 123 11 23 227 10 216 2.36e-11 65.5
MS.gene25386.t1 AT4G15060 25.592 422 241 19 7 394 11 393 2.78e-11 65.5
MS.gene25386.t1 AT5G50270 25.472 424 254 14 23 415 2 394 3.85e-11 65.1
MS.gene25386.t1 AT1G55660 25.272 459 295 20 20 447 50 491 4.19e-11 65.5
MS.gene25386.t1 AT5G35995 29.707 239 133 9 23 254 5 215 8.43e-11 64.3
MS.gene25386.t1 AT5G35995 29.707 239 133 9 23 254 5 215 8.43e-11 64.3

Find 106 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTCAACTTCGTTCAAAA+AGG 0.237370 8.2:-39901292 MS.gene25386:CDS
ATGATGTAAATCGCGAAATT+CGG 0.311734 8.2:+39900355 None:intergenic
GACCCAGCTTTATCTTCTTA+AGG 0.328764 8.2:-39899844 None:intergenic
TGATGTAAATCGCGAAATTC+GGG 0.330334 8.2:+39900356 None:intergenic
CCTAGGGCAGCCAGAGATAA+AGG 0.341297 8.2:+39900812 None:intergenic
TGTTGGTAAGTTTCACAGTA+TGG 0.352464 8.2:-39900652 MS.gene25386:CDS
ATGTTTCAACTCAAATCTTC+TGG 0.366123 8.2:-39900315 MS.gene25386:CDS
ATATCCTTCTATGTGAATAT+AGG 0.378662 8.2:+39900038 None:intergenic
TTCTAATACTGCAGTCTTCA+CGG 0.386013 8.2:-39900945 MS.gene25386:intron
CTCGGATGAGGTTGAGAATA+GGG 0.388430 8.2:+39900580 None:intergenic
GAATTAGTTTCCTTTATCTC+TGG 0.402305 8.2:-39900822 MS.gene25386:CDS
TCAAGGACTTGAAGATGAAT+TGG 0.403742 8.2:-39900016 MS.gene25386:CDS
CCTTTATCTCTGGCTGCCCT+AGG 0.405572 8.2:-39900812 MS.gene25386:CDS
GTCTCTCGGCGAAGATATTG+TGG 0.415832 8.2:-39900853 MS.gene25386:CDS
AATTCGGGATAATTTAGAAC+AGG 0.421287 8.2:+39900371 None:intergenic
TATCTCTGGCTGCCCTAGGC+TGG 0.427111 8.2:-39900808 MS.gene25386:CDS
CTGGCCTATATTCACATAGA+AGG 0.430837 8.2:-39900042 MS.gene25386:CDS
TTGGGAGGAAGGACATGATA+TGG 0.433088 8.2:+39901403 None:intergenic
GAAAGTCGAAGTCGAAGATC+TGG 0.433372 8.2:+39901316 None:intergenic
CTCCTAGATATCTTCATCTC+TGG 0.433430 8.2:-39901347 MS.gene25386:CDS
ACTTACTTTAATATAAATGA+AGG 0.456907 8.2:-39900693 MS.gene25386:CDS
TTCCAGAGATGAAGATATCT+AGG 0.456977 8.2:+39901345 None:intergenic
TTCGCATGTCGCGGGATTTG+CGG 0.457078 8.2:+39901238 None:intergenic
CCTCCAATGACGATTTCACT+TGG 0.463758 8.2:-39900716 MS.gene25386:CDS
GAAGAAAACGACGGTGGAGG+AGG 0.471953 8.2:-39901474 MS.gene25386:CDS
GGAAGGTTTCTCGAGAACTT+TGG 0.472309 8.2:+39900737 None:intergenic
TCACCTTAAGAAGATAAAGC+TGG 0.475192 8.2:+39899841 None:intergenic
CTTCAGTGATGGAAAATAAA+CGG 0.475416 8.2:+39900881 None:intergenic
ACCATTGAGAACATGGCAAC+CGG 0.478521 8.2:-39900100 MS.gene25386:CDS
TTGCGACGGAAGTTCTCGTT+GGG 0.479366 8.2:+39901385 None:intergenic
TTCTTGAAGGTGGGGTCCAA+TGG 0.482518 8.2:+39901137 None:intergenic
CACTTCTGTCATCTCGGATG+AGG 0.484315 8.2:+39900568 None:intergenic
AAGATAGAGTTCTTGAAGGT+GGG 0.486485 8.2:+39901128 None:intergenic
ACATGCAAAGTTTCCAGCCT+AGG 0.487752 8.2:+39900795 None:intergenic
ATAGATCCTCGTCATCATCT+AGG 0.488635 8.2:+39900529 None:intergenic
TCTCGGATGAGGTTGAGAAT+AGG 0.495111 8.2:+39900579 None:intergenic
AGTCCAAGTGAAATCGTCAT+TGG 0.497220 8.2:+39900713 None:intergenic
CACCTTAAGAAGATAAAGCT+GGG 0.498310 8.2:+39899842 None:intergenic
TGCCATGTTCTCAATGGTAG+TGG 0.499626 8.2:+39900105 None:intergenic
CTTCTATGTGAATATAGGCC+AGG 0.513224 8.2:+39900043 None:intergenic
ACGTGGATTCTTGCTGCCAT+TGG 0.513966 8.2:-39901153 MS.gene25386:CDS
GAAGAAGAAAACGACGGTGG+AGG 0.517300 8.2:-39901477 MS.gene25386:CDS
ATTCACATAGAAGGATATCA+AGG 0.519668 8.2:-39900033 MS.gene25386:CDS
TACAAAGATAGAGTTCTTGA+AGG 0.525754 8.2:+39901124 None:intergenic
GAAATTCACTTCTGTCATCT+CGG 0.527470 8.2:+39900562 None:intergenic
AAGCTGGGTCAAAATTAAGT+TGG 0.530087 8.2:+39899857 None:intergenic
GGAAGTTCTCGTTGGGAGGA+AGG 0.530993 8.2:+39901392 None:intergenic
ATAAAGGAAACTAATTCAGT+TGG 0.537900 8.2:+39900828 None:intergenic
ATATGGCATAGTACAAAGTC+CGG 0.539413 8.2:+39901420 None:intergenic
CATGCAAAGTTTCCAGCCTA+GGG 0.543778 8.2:+39900796 None:intergenic
CCAAGTGAAATCGTCATTGG+AGG 0.548354 8.2:+39900716 None:intergenic
GTTGCGACGGAAGTTCTCGT+TGG 0.548976 8.2:+39901384 None:intergenic
GCACTAACCTCGTTTCCCTA+AGG 0.549237 8.2:-39901056 MS.gene25386:intron
TCAGAAAGTGTTGTTGACTC+CGG 0.549326 8.2:+39900081 None:intergenic
TCCGGTTGCCATGTTCTCAA+TGG 0.550198 8.2:+39900099 None:intergenic
CGACAGGTTGAGCACTCTAC+CGG 0.550990 8.2:-39901439 MS.gene25386:CDS
GACGGAACCTTCGCATGTCG+CGG 0.552064 8.2:+39901229 None:intergenic
TTTAGAACAGGGGCATGAGT+TGG 0.553956 8.2:+39900383 None:intergenic
ACGGAACCTTCGCATGTCGC+GGG 0.556611 8.2:+39901230 None:intergenic
CGCTGAATCCACGATGGCGG+AGG 0.561894 8.2:-39901517 None:intergenic
AACAAATTCAGTTTCAGCCA+CGG 0.563124 8.2:+39900605 None:intergenic
AACCACTACCATTGAGAACA+TGG 0.563418 8.2:-39900107 MS.gene25386:CDS
GAGGCGATGACCTCACCGAC+AGG 0.564999 8.2:-39901455 MS.gene25386:CDS
TCTCTCGGCGAAGATATTGT+GGG 0.566500 8.2:-39900852 MS.gene25386:CDS
AGAGACAACGTCTTCAGTGA+TGG 0.567151 8.2:+39900870 None:intergenic
GCAAATCCCGCGACATGCGA+AGG 0.571421 8.2:-39901236 MS.gene25386:CDS
AAAGATAGAGTTCTTGAAGG+TGG 0.577183 8.2:+39901127 None:intergenic
GACAGAAGTGAATTTCATAA+CGG 0.579647 8.2:-39900555 MS.gene25386:CDS
TCACTGAAGACGTTGTCTCT+CGG 0.580179 8.2:-39900867 MS.gene25386:CDS
AGATAGAGTTCTTGAAGGTG+GGG 0.582541 8.2:+39901129 None:intergenic
GAAAATAAACGGAGTTTACA+CGG 0.584017 8.2:+39900892 None:intergenic
AGAAGATTTGAGTTGAAACA+TGG 0.586977 8.2:+39900317 None:intergenic
GTGAAATCGTCATTGGAGGA+AGG 0.593833 8.2:+39900720 None:intergenic
GACGAGGCTCATGGTTGCGA+CGG 0.594269 8.2:+39901371 None:intergenic
CGACGGAAGTTCTCGTTGGG+AGG 0.597115 8.2:+39901388 None:intergenic
GATTTGAGTTGAAACATGGA+AGG 0.600986 8.2:+39900321 None:intergenic
TGGCACATGTCAGAGCCCCT+TGG 0.606191 8.2:+39899877 None:intergenic
CTGAGTTGTAACTCATCGCG+TGG 0.606212 8.2:+39901186 None:intergenic
GTTGTGTCATTCGACTTCAA+AGG 0.607118 8.2:-39900499 MS.gene25386:intron
TCTAGGAGAGACGAGGCTCA+TGG 0.609801 8.2:+39901362 None:intergenic
TGAATGTCTCAAATCCCACC+TGG 0.614417 8.2:-39900061 MS.gene25386:CDS
TGGTAAGTTTCACAGTATGG+TGG 0.620888 8.2:-39900649 MS.gene25386:CDS
CGGTAGAGTGCTCAACCTGT+CGG 0.621178 8.2:+39901440 None:intergenic
TAGATATCTTCATCTCTGGA+AGG 0.622526 8.2:-39901343 MS.gene25386:CDS
CAATTGATGAAGAGTGAACG+AGG 0.626927 8.2:+39901078 None:intergenic
AAAGATTAAGAGACATACCA+AGG 0.629448 8.2:-39899894 MS.gene25386:CDS
TTCGGGATAATTTAGAACAG+GGG 0.629854 8.2:+39900373 None:intergenic
TATGTGAATATAGGCCAGGT+GGG 0.630335 8.2:+39900047 None:intergenic
CGGCCTCCTAGATGATGACG+AGG 0.633593 8.2:-39900535 MS.gene25386:CDS
AAGATTAAGAGACATACCAA+GGG 0.634798 8.2:-39899893 MS.gene25386:CDS
TCTCATAATTCAGAGCAACA+AGG 0.643040 8.2:-39900258 MS.gene25386:intron
ATTCGGGATAATTTAGAACA+GGG 0.649291 8.2:+39900372 None:intergenic
CATCAAATGTGAGAGTGAGA+CGG 0.650223 8.2:+39901211 None:intergenic
CTATGTGAATATAGGCCAGG+TGG 0.652220 8.2:+39900046 None:intergenic
TTCAATGGACAAAACAGACA+AGG 0.659476 8.2:-39899929 MS.gene25386:CDS
GAGTGCTCAACCTGTCGGTG+AGG 0.660183 8.2:+39901445 None:intergenic
AACTCAGGAATGCTGAAACG+TGG 0.660563 8.2:-39901170 MS.gene25386:CDS
AAATTTACCTTAGGGAAACG+AGG 0.660954 8.2:+39901049 None:intergenic
GATCCTCGTCATCATCTAGG+AGG 0.666579 8.2:+39900532 None:intergenic
CACGCGATGAGTTACAACTC+AGG 0.685874 8.2:-39901185 MS.gene25386:CDS
TTATGAGAACTTGAAGCATG+TGG 0.695487 8.2:+39900273 None:intergenic
GCAGAAGAAGAAAACGACGG+TGG 0.701063 8.2:-39901480 MS.gene25386:CDS
GAAGATATCTAGGAGAGACG+AGG 0.711896 8.2:+39901355 None:intergenic
AAAGCAGAAGAAGAAAACGA+CGG 0.714159 8.2:-39901483 MS.gene25386:CDS
AGATTAAGAGACATACCAAG+GGG 0.741057 8.2:-39899892 MS.gene25386:CDS
AATTGATGAAGAGTGAACGA+GGG 0.761342 8.2:+39901079 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAATTCAAACATTCTT+TGG - chr8.2:39901130-39901149 MS.gene25386:CDS 15.0%
!! ACTTACTTTAATATAAATGA+AGG - chr8.2:39900657-39900676 MS.gene25386:CDS 15.0%
!!! TATTTTTGTTGATATTTCAA+TGG - chr8.2:39901406-39901425 MS.gene25386:CDS 15.0%
!!! AAATGAAGGATGTTTTAAAT+TGG - chr8.2:39900671-39900690 MS.gene25386:CDS 20.0%
!!! AATGAAGGATGTTTTAAATT+GGG - chr8.2:39900672-39900691 MS.gene25386:CDS 20.0%
!!! ACTGAATATATTTTATGGAA+TGG - chr8.2:39901362-39901381 MS.gene25386:CDS 20.0%
!!! CATTCACTGAATATATTTTA+TGG - chr8.2:39901357-39901376 MS.gene25386:CDS 20.0%
! ATAAAGGAAACTAATTCAGT+TGG + chr8.2:39900525-39900544 None:intergenic 25.0%
! ATATCCTTCTATGTGAATAT+AGG + chr8.2:39901315-39901334 None:intergenic 25.0%
! ATCTGCAAAAATTTACCTTA+GGG + chr8.2:39900312-39900331 None:intergenic 25.0%
! TAATTCAAACATTCTTTGGT+AGG - chr8.2:39901134-39901153 MS.gene25386:CDS 25.0%
! TATCTGCAAAAATTTACCTT+AGG + chr8.2:39900313-39900332 None:intergenic 25.0%
!!! ATGTTTTTACATGTGTAGTT+TGG - chr8.2:39900898-39900917 MS.gene25386:CDS 25.0%
!!! TGTACACAAGTATATTTTTG+AGG - chr8.2:39901187-39901206 MS.gene25386:CDS 25.0%
AAAGATTAAGAGACATACCA+AGG - chr8.2:39901456-39901475 MS.gene25386:CDS 30.0%
AAGATTAAGAGACATACCAA+GGG - chr8.2:39901457-39901476 MS.gene25386:CDS 30.0%
ATGATGTAAATCGCGAAATT+CGG + chr8.2:39900998-39901017 None:intergenic 30.0%
ATGTTTCAACTCAAATCTTC+TGG - chr8.2:39901035-39901054 MS.gene25386:intron 30.0%
ATTCACATAGAAGGATATCA+AGG - chr8.2:39901317-39901336 MS.gene25386:CDS 30.0%
GAAAATAAACGGAGTTTACA+CGG + chr8.2:39900461-39900480 None:intergenic 30.0%
GACAGAAGTGAATTTCATAA+CGG - chr8.2:39900795-39900814 MS.gene25386:CDS 30.0%
TACAAAGATAGAGTTCTTGA+AGG + chr8.2:39900229-39900248 None:intergenic 30.0%
! AATTCGGGATAATTTAGAAC+AGG + chr8.2:39900982-39901001 None:intergenic 30.0%
! ATTCGGGATAATTTAGAACA+GGG + chr8.2:39900981-39901000 None:intergenic 30.0%
! CTTCAGTGATGGAAAATAAA+CGG + chr8.2:39900472-39900491 None:intergenic 30.0%
! GAATTAGTTTCCTTTATCTC+TGG - chr8.2:39900528-39900547 MS.gene25386:CDS 30.0%
!! AGAAGATTTGAGTTGAAACA+TGG + chr8.2:39901036-39901055 None:intergenic 30.0%
!! CTCTTAATCTTTTGAGAGAA+CGG + chr8.2:39901448-39901467 None:intergenic 30.0%
!! TGTTTTAAATTGGGCATTGT+TGG - chr8.2:39900681-39900700 MS.gene25386:CDS 30.0%
!!! ACAAGTATATTTTTGAGGCA+CGG - chr8.2:39901192-39901211 MS.gene25386:CDS 30.0%
AAAGATAGAGTTCTTGAAGG+TGG + chr8.2:39900226-39900245 None:intergenic 35.0%
AAAGCAGAAGAAGAAAACGA+CGG - chr8.2:39899867-39899886 MS.gene25386:CDS 35.0%
AAATTTACCTTAGGGAAACG+AGG + chr8.2:39900304-39900323 None:intergenic 35.0%
AACAAATTCAGTTTCAGCCA+CGG + chr8.2:39900748-39900767 None:intergenic 35.0%
AAGATAGAGTTCTTGAAGGT+GGG + chr8.2:39900225-39900244 None:intergenic 35.0%
AAGCTGGGTCAAAATTAAGT+TGG + chr8.2:39901496-39901515 None:intergenic 35.0%
AATTGATGAAGAGTGAACGA+GGG + chr8.2:39900274-39900293 None:intergenic 35.0%
AGATTAAGAGACATACCAAG+GGG - chr8.2:39901458-39901477 MS.gene25386:CDS 35.0%
ATATGGCATAGTACAAAGTC+CGG + chr8.2:39899933-39899952 None:intergenic 35.0%
GAAATTCACTTCTGTCATCT+CGG + chr8.2:39900791-39900810 None:intergenic 35.0%
TACACGATAACAACAAGTGA+TGG + chr8.2:39900937-39900956 None:intergenic 35.0%
TAGATATCTTCATCTCTGGA+AGG - chr8.2:39900007-39900026 MS.gene25386:CDS 35.0%
TCAAGGACTTGAAGATGAAT+TGG - chr8.2:39901334-39901353 MS.gene25386:CDS 35.0%
TCTCATAATTCAGAGCAACA+AGG - chr8.2:39901092-39901111 MS.gene25386:CDS 35.0%
TGATGTAAATCGCGAAATTC+GGG + chr8.2:39900997-39901016 None:intergenic 35.0%
TTATGAGAACTTGAAGCATG+TGG + chr8.2:39901080-39901099 None:intergenic 35.0%
TTCAATGGACAAAACAGACA+AGG - chr8.2:39901421-39901440 MS.gene25386:CDS 35.0%
TTCCAGAGATGAAGATATCT+AGG + chr8.2:39900008-39900027 None:intergenic 35.0%
TTCTAATACTGCAGTCTTCA+CGG - chr8.2:39900405-39900424 MS.gene25386:intron 35.0%
! TCGTTTTCTTCTTCTGCTTT+TGG + chr8.2:39899867-39899886 None:intergenic 35.0%
! TGTTGGTAAGTTTCACAGTA+TGG - chr8.2:39900698-39900717 MS.gene25386:CDS 35.0%
! TTCGGGATAATTTAGAACAG+GGG + chr8.2:39900980-39900999 None:intergenic 35.0%
!! GATTTGAGTTGAAACATGGA+AGG + chr8.2:39901032-39901051 None:intergenic 35.0%
!! TTTTCTTCTTCTGCTTTTGG+TGG + chr8.2:39899864-39899883 None:intergenic 35.0%
!!! TATATTTTTGAGGCACGGTA+TGG - chr8.2:39901197-39901216 MS.gene25386:CDS 35.0%
AACCACTACCATTGAGAACA+TGG - chr8.2:39901243-39901262 MS.gene25386:CDS 40.0%
AGATAGAGTTCTTGAAGGTG+GGG + chr8.2:39900224-39900243 None:intergenic 40.0%
AGTCCAAGTGAAATCGTCAT+TGG + chr8.2:39900640-39900659 None:intergenic 40.0%
ATAGATCCTCGTCATCATCT+AGG + chr8.2:39900824-39900843 None:intergenic 40.0%
CAATTGATGAAGAGTGAACG+AGG + chr8.2:39900275-39900294 None:intergenic 40.0%
CATCAAATGTGAGAGTGAGA+CGG + chr8.2:39900142-39900161 None:intergenic 40.0%
CCACTCAACTTCGTTCAAAA+AGG - chr8.2:39900058-39900077 MS.gene25386:CDS 40.0%
CTCCTAGATATCTTCATCTC+TGG - chr8.2:39900003-39900022 MS.gene25386:CDS 40.0%
CTGGCCTATATTCACATAGA+AGG - chr8.2:39901308-39901327 MS.gene25386:CDS 40.0%
CTTCTATGTGAATATAGGCC+AGG + chr8.2:39901310-39901329 None:intergenic 40.0%
TATGTGAATATAGGCCAGGT+GGG + chr8.2:39901306-39901325 None:intergenic 40.0%
TGGTAAGTTTCACAGTATGG+TGG - chr8.2:39900701-39900720 MS.gene25386:CDS 40.0%
! CCTTTTTGAACGAAGTTGAG+TGG + chr8.2:39900061-39900080 None:intergenic 40.0%
! GTTGTGTCATTCGACTTCAA+AGG - chr8.2:39900851-39900870 MS.gene25386:CDS 40.0%
! GTTTCTTGATGTCTTTTCCG+TGG - chr8.2:39900728-39900747 MS.gene25386:CDS 40.0%
!! TCAGAAAGTGTTGTTGACTC+CGG + chr8.2:39901272-39901291 None:intergenic 40.0%
AACTCAGGAATGCTGAAACG+TGG - chr8.2:39900180-39900199 MS.gene25386:intron 45.0%
ACATGCAAAGTTTCCAGCCT+AGG + chr8.2:39900558-39900577 None:intergenic 45.0%
ACCATTGAGAACATGGCAAC+CGG - chr8.2:39901250-39901269 MS.gene25386:CDS 45.0%
AGAGACAACGTCTTCAGTGA+TGG + chr8.2:39900483-39900502 None:intergenic 45.0%
CATGCAAAGTTTCCAGCCTA+GGG + chr8.2:39900557-39900576 None:intergenic 45.0%
CCAAGTGAAATCGTCATTGG+AGG + chr8.2:39900637-39900656 None:intergenic 45.0%
CCTCCAATGACGATTTCACT+TGG - chr8.2:39900634-39900653 MS.gene25386:CDS 45.0%
CTATGTGAATATAGGCCAGG+TGG + chr8.2:39901307-39901326 None:intergenic 45.0%
CTCGGATGAGGTTGAGAATA+GGG + chr8.2:39900773-39900792 None:intergenic 45.0%
GAAAGTCGAAGTCGAAGATC+TGG + chr8.2:39900037-39900056 None:intergenic 45.0%
GAAGATATCTAGGAGAGACG+AGG + chr8.2:39899998-39900017 None:intergenic 45.0%
GGAAGGTTTCTCGAGAACTT+TGG + chr8.2:39900616-39900635 None:intergenic 45.0%
GTGAAATCGTCATTGGAGGA+AGG + chr8.2:39900633-39900652 None:intergenic 45.0%
GTTCCTCCTACAACCAACAA+GGG + chr8.2:39901226-39901245 None:intergenic 45.0%
TCACTGAAGACGTTGTCTCT+CGG - chr8.2:39900483-39900502 MS.gene25386:intron 45.0%
TCTCGGATGAGGTTGAGAAT+AGG + chr8.2:39900774-39900793 None:intergenic 45.0%
TCTCTCGGCGAAGATATTGT+GGG - chr8.2:39900498-39900517 MS.gene25386:intron 45.0%
TGAATGTCTCAAATCCCACC+TGG - chr8.2:39901289-39901308 MS.gene25386:CDS 45.0%
TGCCATGTTCTCAATGGTAG+TGG + chr8.2:39901248-39901267 None:intergenic 45.0%
TTGGGAGGAAGGACATGATA+TGG + chr8.2:39899950-39899969 None:intergenic 45.0%
TTGTTATCGTGTACGTGCAG+TGG - chr8.2:39900944-39900963 MS.gene25386:intron 45.0%
! TTTAGAACAGGGGCATGAGT+TGG + chr8.2:39900970-39900989 None:intergenic 45.0%
ACGTGGATTCTTGCTGCCAT+TGG - chr8.2:39900197-39900216 MS.gene25386:intron 50.0%
CACGCGATGAGTTACAACTC+AGG - chr8.2:39900165-39900184 MS.gene25386:intron 50.0%
CACTTCTGTCATCTCGGATG+AGG + chr8.2:39900785-39900804 None:intergenic 50.0%
CTGAGTTGTAACTCATCGCG+TGG + chr8.2:39900167-39900186 None:intergenic 50.0%
GAAGAAGAAAACGACGGTGG+AGG - chr8.2:39899873-39899892 MS.gene25386:CDS 50.0%
GATCCTCGTCATCATCTAGG+AGG + chr8.2:39900821-39900840 None:intergenic 50.0%
GCACTAACCTCGTTTCCCTA+AGG - chr8.2:39900294-39900313 MS.gene25386:CDS 50.0%
GCAGAAGAAGAAAACGACGG+TGG - chr8.2:39899870-39899889 MS.gene25386:CDS 50.0%
GGTTCCTCCTACAACCAACA+AGG + chr8.2:39901227-39901246 None:intergenic 50.0%
GTCTCTCGGCGAAGATATTG+TGG - chr8.2:39900497-39900516 MS.gene25386:intron 50.0%
TCCGGTTGCCATGTTCTCAA+TGG + chr8.2:39901254-39901273 None:intergenic 50.0%
TTCTTGAAGGTGGGGTCCAA+TGG + chr8.2:39900216-39900235 None:intergenic 50.0%
TTGCGACGGAAGTTCTCGTT+GGG + chr8.2:39899968-39899987 None:intergenic 50.0%
! TGGTGTCCCTTGTTGGTTGT+AGG - chr8.2:39901217-39901236 MS.gene25386:CDS 50.0%
! TGTCCCTTGTTGGTTGTAGG+AGG - chr8.2:39901220-39901239 MS.gene25386:CDS 50.0%
CACGGTATGGTGTCCCTTGT+TGG - chr8.2:39901210-39901229 MS.gene25386:CDS 55.0%
CCTAGGGCAGCCAGAGATAA+AGG + chr8.2:39900541-39900560 None:intergenic 55.0%
CCTTTATCTCTGGCTGCCCT+AGG - chr8.2:39900538-39900557 MS.gene25386:CDS 55.0%
GAAGAAAACGACGGTGGAGG+AGG - chr8.2:39899876-39899895 MS.gene25386:CDS 55.0%
GGAAGTTCTCGTTGGGAGGA+AGG + chr8.2:39899961-39899980 None:intergenic 55.0%
GTTGCGACGGAAGTTCTCGT+TGG + chr8.2:39899969-39899988 None:intergenic 55.0%
TCTAGGAGAGACGAGGCTCA+TGG + chr8.2:39899991-39900010 None:intergenic 55.0%
TTCGCATGTCGCGGGATTTG+CGG + chr8.2:39900115-39900134 None:intergenic 55.0%
! CGACAGGTTGAGCACTCTAC+CGG - chr8.2:39899911-39899930 MS.gene25386:CDS 55.0%
! CGGTAGAGTGCTCAACCTGT+CGG + chr8.2:39899913-39899932 None:intergenic 55.0%
ACGGAACCTTCGCATGTCGC+GGG + chr8.2:39900123-39900142 None:intergenic 60.0%
CGACGGAAGTTCTCGTTGGG+AGG + chr8.2:39899965-39899984 None:intergenic 60.0%
GACGAGGCTCATGGTTGCGA+CGG + chr8.2:39899982-39900001 None:intergenic 60.0%
GACGGAACCTTCGCATGTCG+CGG + chr8.2:39900124-39900143 None:intergenic 60.0%
GAGTGCTCAACCTGTCGGTG+AGG + chr8.2:39899908-39899927 None:intergenic 60.0%
GCAAATCCCGCGACATGCGA+AGG - chr8.2:39900114-39900133 MS.gene25386:CDS 60.0%
TATCTCTGGCTGCCCTAGGC+TGG - chr8.2:39900542-39900561 MS.gene25386:CDS 60.0%
TGGCACATGTCAGAGCCCCT+TGG + chr8.2:39901476-39901495 None:intergenic 60.0%
! CGGCCTCCTAGATGATGACG+AGG - chr8.2:39900815-39900834 MS.gene25386:CDS 60.0%
GAGGCGATGACCTCACCGAC+AGG - chr8.2:39899895-39899914 MS.gene25386:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 39899846 39901526 39899846 ID=MS.gene25386
chr8.2 mRNA 39899846 39901526 39899846 ID=MS.gene25386.t1;Parent=MS.gene25386
chr8.2 exon 39901057 39901526 39901057 ID=MS.gene25386.t1.exon1;Parent=MS.gene25386.t1
chr8.2 CDS 39901057 39901526 39901057 ID=cds.MS.gene25386.t1;Parent=MS.gene25386.t1
chr8.2 exon 39900500 39900953 39900500 ID=MS.gene25386.t1.exon2;Parent=MS.gene25386.t1
chr8.2 CDS 39900500 39900953 39900500 ID=cds.MS.gene25386.t1;Parent=MS.gene25386.t1
chr8.2 exon 39900259 39900408 39900259 ID=MS.gene25386.t1.exon3;Parent=MS.gene25386.t1
chr8.2 CDS 39900259 39900408 39900259 ID=cds.MS.gene25386.t1;Parent=MS.gene25386.t1
chr8.2 exon 39899846 39900133 39899846 ID=MS.gene25386.t1.exon4;Parent=MS.gene25386.t1
chr8.2 CDS 39899846 39900133 39899846 ID=cds.MS.gene25386.t1;Parent=MS.gene25386.t1
Gene Sequence

>MS.gene25386

ATGGCGGAGGAGTCACCACCAAAAGCAGAAGAAGAAAACGACGGTGGAGGAGGCGATGACCTCACCGACAGGTTGAGCACTCTACCGGACTTTGTACTATGCCATATCATGTCCTTCCTCCCAACGAGAACTTCCGTCGCAACCATGAGCCTCGTCTCTCCTAGATATCTTCATCTCTGGAAGGATCTCCAGATCTTCGACTTCGACTTTCACCACTCAACTTCGTTCAAAAAGGTTGCATTATTCATTAACTCTGTTCTCACTCTCCGCAAATCCCGCGACATGCGAAGGTTCCGTCTCACTCTCACATTTGATGATCCACGCGATGAGTTACAACTCAGGAATGCTGAAACGTGGATTCTTGCTGCCATTGGACCCCACCTTCAAGAACTCTATCTTTGTATTGACGAGTCTTGTATTGACATCCCTCGTTCACTCTTCATCAATTGCACTAACCTCGTTTCCCTAAGGTAAATTTTTGCAGATACTCATGTTTTAATGAAAACTCAGAATGATTTGTTTTTATGTTCAAACTCACTTTTACTTTCGAATCATATTTTTCTAATACTGCAGTCTTCACGGTTCAATCGAAACAGCACACTGCAATCTTGACCGTGTAAACTCCGTTTATTTTCCATCACTGAAGACGTTGTCTCTCGGCGAAGATATTGTGGGTCCAACTGAATTAGTTTCCTTTATCTCTGGCTGCCCTAGGCTGGAAACTTTGCATGTTTACTTTTACATTGTCTCTGAAGAAATGTCTTTCACCAAAGTTCTCGAGAAACCTTCCTCCAATGACGATTTCACTTGGACTTACTTTAATATAAATGAAGGATGTTTTAAATTGGGCATTGTTGGTAAGTTTCACAGTATGGTGGAAGCGTTTCTTGATGTCTTTTCCGTGGCTGAAACTGAATTTGTTGACCCTATTCTCAACCTCATCCGAGATGACAGAAGTGAATTTCATAACGGCCTCCTAGATGATGACGAGGATCTATATCTAGAGTTGTGTCATTCGACTTCAAAGGTGAAGTTTTACTTTTATGCTTTATATGTTTTTACATGTGTAGTTTGGTTCTAATATTGCGCCATCACTTGTTGTTATCGTGTACGTGCAGTGGCCAACTCATGCCCCTGTTCTAAATTATCCCGAATTTCGCGATTTACATCATCTGAAGTTCATCCTTCCATGTTTCAACTCAAATCTTCTGGTCAATGTTCTTGAGCAATGCCACATGCTTCAAGTTCTCATAATTCAGAGCAACAAGGTTTGTTGAATTTTCAAATTTAATTCAAACATTCTTTGGTAGGTCTATATATACATGCATGTATGTCTCTATCTGTACACAAGTATATTTTTGAGGCACGGTATGGTGTCCCTTGTTGGTTGTAGGAGGAACCACTACCATTGAGAACATGGCAACCGGAGTCAACAACACTTTCTGAATGTCTCAAATCCCACCTGGCCTATATTCACATAGAAGGATATCAAGGACTTGAAGATGAATTGGCATTCACTGAATATATTTTATGGAATGGAGTTGTTTTGAAGACAATGCTTATTTTTGTTGATATTTCAATGGACAAAACAGACAAGGACCGTTCTCTCAAAAGATTAAGAGACATACCAAGGGGCTCTGACATGTGCCAACTTAATTTTGACCCAGCTTTATCTTCTTAA
Protein sequence

>MS.gene25386.t1

MAEESPPKAEEENDGGGGDDLTDRLSTLPDFVLCHIMSFLPTRTSVATMSLVSPRYLHLWKDLQIFDFDFHHSTSFKKVALFINSVLTLRKSRDMRRFRLTLTFDDPRDELQLRNAETWILAAIGPHLQELYLCIDESCIDIPRSLFINCTNLVSLSLHGSIETAHCNLDRVNSVYFPSLKTLSLGEDIVGPTELVSFISGCPRLETLHVYFYIVSEEMSFTKVLEKPSSNDDFTWTYFNINEGCFKLGIVGKFHSMVEAFLDVFSVAETEFVDPILNLIRDDRSEFHNGLLDDDEDLYLELCHSTSKWPTHAPVLNYPEFRDLHHLKFILPCFNSNLLVNVLEQCHMLQVLIIQSNKEEPLPLRTWQPESTTLSECLKSHLAYIHIEGYQGLEDELAFTEYILWNGVVLKTMLIFVDISMDKTDKDRSLKRLRDIPRGSDMCQLNFDPALSS