AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062537

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062537.t1 MTR_8g062300 56.478 494 162 10 1 453 1 482 9.56e-172 491
MS.gene062537.t1 MTR_8g062080 55.940 463 181 9 1 453 1 450 7.52e-163 467
MS.gene062537.t1 MTR_8g070950 56.596 470 154 11 1 452 1 438 6.63e-162 464
MS.gene062537.t1 MTR_8g062310 52.784 449 186 7 1 448 5 428 6.67e-152 439
MS.gene062537.t1 MTR_8g062010 55.257 447 162 10 23 452 9 434 9.47e-152 438
MS.gene062537.t1 MTR_8g469020 49.787 470 177 13 22 452 6 455 3.74e-138 404
MS.gene062537.t1 MTR_8g058965 48.347 484 187 12 9 453 2 461 1.41e-130 385
MS.gene062537.t1 MTR_4g050250 47.178 443 162 13 1 394 1 420 1.87e-110 333
MS.gene062537.t1 MTR_8g467790 45.098 408 145 12 23 402 148 504 1.22e-98 305
MS.gene062537.t1 MTR_8g468810 38.969 485 237 15 1 448 1 463 1.43e-86 272
MS.gene062537.t1 MTR_8g062270 38.229 463 229 14 20 447 13 453 3.31e-81 258
MS.gene062537.t1 MTR_2g083840 39.651 459 224 12 22 448 20 457 3.32e-80 256
MS.gene062537.t1 MTR_2g086020 83.448 145 24 0 308 452 175 319 4.19e-75 238
MS.gene062537.t1 MTR_2g086020 60.959 146 49 2 49 190 1 142 7.69e-48 167
MS.gene062537.t1 MTR_4g032730 36.456 491 234 15 9 447 2 466 5.01e-75 243
MS.gene062537.t1 MTR_8g058970 36.926 501 236 19 3 448 2 477 4.14e-72 235
MS.gene062537.t1 MTR_8g467800 38.608 474 230 17 8 448 8 453 6.04e-72 234
MS.gene062537.t1 MTR_8g468840 36.752 468 223 15 21 446 12 448 4.21e-70 229
MS.gene062537.t1 MTR_8g014260 38.731 457 228 20 23 448 16 451 1.54e-69 228
MS.gene062537.t1 MTR_8g468760 36.443 461 226 17 10 447 2 418 2.52e-63 211
MS.gene062537.t1 MTR_8g468810 38.931 393 183 14 1 358 1 371 3.77e-63 209
MS.gene062537.t1 MTR_5g092960 33.895 475 256 12 11 447 7 461 6.60e-63 211
MS.gene062537.t1 MTR_8g445780 36.235 425 212 16 1 401 1 390 4.82e-57 193
MS.gene062537.t1 MTR_8g468810 41.158 311 141 11 1 281 1 299 6.16e-54 183
MS.gene062537.t1 MTR_8g468810 40.836 311 142 11 1 281 1 299 6.80e-54 183
MS.gene062537.t1 MTR_8g468810 41.158 311 141 11 1 281 1 299 1.21e-53 183
MS.gene062537.t1 MTR_8g014160 34.273 461 210 16 14 448 32 425 7.85e-51 178
MS.gene062537.t1 MTR_5g092960 32.984 382 205 10 11 358 7 371 1.97e-40 149
MS.gene062537.t1 MTR_4g024710 35.880 301 158 11 166 448 64 347 5.68e-40 147
MS.gene062537.t1 MTR_8g467800 39.130 299 144 13 8 281 8 293 3.58e-35 134
MS.gene062537.t1 MTR_8g077460 47.651 149 74 4 301 448 230 375 4.48e-34 131
MS.gene062537.t1 MTR_8g077460 36.946 203 110 7 23 212 10 207 3.18e-23 101
MS.gene062537.t1 MTR_8g014210 39.908 218 112 8 233 448 31 231 6.79e-32 122
MS.gene062537.t1 MTR_3g099800 71.605 81 22 1 360 439 19 99 2.47e-30 113
MS.gene062537.t1 MTR_7g098510 28.211 436 241 14 23 448 18 391 4.50e-29 118
MS.gene062537.t1 MTR_4g024745 33.793 290 145 12 166 431 18 284 1.02e-26 109
MS.gene062537.t1 MTR_1g041245 29.279 444 230 18 22 448 10 386 3.97e-26 109
MS.gene062537.t1 MTR_5g021550 43.421 152 72 7 17 156 13 162 5.87e-25 100
MS.gene062537.t1 MTR_5g008950 26.424 439 234 17 25 448 1 365 5.02e-23 100
MS.gene062537.t1 MTR_3g107160 26.244 442 258 14 15 448 2 383 9.16e-23 100
MS.gene062537.t1 MTR_8g014290 36.601 153 77 5 23 157 10 160 8.59e-21 89.4
MS.gene062537.t1 MTR_7g026500 25.901 444 247 16 23 447 14 394 6.84e-20 91.7
MS.gene062537.t1 MTR_7g098480 27.211 441 237 15 22 447 17 388 7.80e-20 92.0
MS.gene062537.t1 MTR_3g107100 25.828 453 256 16 19 450 7 400 1.70e-19 90.5
MS.gene062537.t1 MTR_7g098570 26.835 436 241 16 22 444 19 389 3.70e-19 90.1
MS.gene062537.t1 MTR_7g115270 25.909 440 257 16 23 445 12 399 3.96e-19 89.4
MS.gene062537.t1 MTR_5g080710 27.517 447 262 17 23 440 33 446 6.99e-19 89.4
MS.gene062537.t1 MTR_2g007450 28.480 467 239 24 16 445 9 417 3.17e-18 87.0
MS.gene062537.t1 MTR_5g084070 27.064 436 279 14 19 440 9 419 3.21e-18 87.0
MS.gene062537.t1 MTR_7g033990 27.169 438 226 19 23 440 11 375 4.80e-18 86.3
MS.gene062537.t1 MTR_1g085120 26.106 452 250 17 16 448 85 471 5.72e-18 86.7
MS.gene062537.t1 MTR_5g073420 26.087 414 219 16 23 422 11 351 5.92e-18 85.9
MS.gene062537.t1 MTR_1g085120 26.106 452 250 17 16 448 160 546 6.97e-18 86.7
MS.gene062537.t1 MTR_7g056447 32.618 233 127 10 23 248 9 218 3.57e-17 82.0
MS.gene062537.t1 MTR_6g040270 25.858 437 240 21 25 445 10 378 2.43e-16 80.9
MS.gene062537.t1 MTR_7g055943 23.663 486 259 22 3 448 8 421 2.53e-16 81.3
MS.gene062537.t1 MTR_7g032380 34.375 192 109 7 23 208 11 191 1.19e-15 78.6
MS.gene062537.t1 MTR_4g024720 39.231 130 56 7 8 126 10 127 1.48e-15 75.9
MS.gene062537.t1 MTR_3g036250 24.186 430 250 14 22 445 4 363 4.00e-15 77.0
MS.gene062537.t1 MTR_3g107120 26.742 445 239 15 23 445 9 388 2.31e-14 75.1
MS.gene062537.t1 MTR_4g098590 25.735 408 232 15 25 415 1 354 1.03e-13 72.8
MS.gene062537.t1 MTR_8g014230 44.565 92 46 3 357 448 18 104 1.36e-13 67.0
MS.gene062537.t1 MTR_3g040700 24.112 394 222 13 22 407 42 366 1.43e-13 72.8
MS.gene062537.t1 MTR_6g022500 25.240 416 223 19 19 415 97 443 2.35e-13 72.4
MS.gene062537.t1 MTR_3g052990 33.702 181 114 4 6 186 11 185 4.99e-13 71.2
MS.gene062537.t1 MTR_0009s0080 25.185 405 226 15 28 415 23 367 5.35e-13 70.9
MS.gene062537.t1 MTR_3g052940 32.597 181 116 4 6 186 11 185 1.42e-12 69.7
MS.gene062537.t1 MTR_3g052940 32.597 181 116 4 6 186 11 185 1.69e-12 69.7
MS.gene062537.t1 MTR_3g052940 32.597 181 116 4 6 186 11 185 1.70e-12 69.7
MS.gene062537.t1 MTR_5g083890 25.170 441 240 16 23 430 6 389 1.96e-12 69.3
MS.gene062537.t1 MTR_4g056030 25.806 465 271 18 18 442 4 434 1.96e-12 69.3
MS.gene062537.t1 MTR_8g085430 32.512 203 107 9 22 208 10 198 2.07e-12 68.6
MS.gene062537.t1 MTR_1g053025 25.397 441 255 20 23 445 2 386 4.02e-12 68.2
MS.gene062537.t1 MTR_4g093690 29.902 204 125 8 19 214 14 207 4.04e-12 67.0
MS.gene062537.t1 MTR_1g038710 25.858 437 256 19 23 417 19 429 4.85e-12 68.2
MS.gene062537.t1 MTR_6g022360 23.218 435 262 16 19 447 39 407 1.43e-11 66.2
MS.gene062537.t1 MTR_5g008920 32.821 195 108 8 23 208 9 189 2.36e-11 65.5
MS.gene062537.t1 MTR_2g007500 31.472 197 101 7 22 208 13 185 2.89e-11 65.1
MS.gene062537.t1 MTR_3g108150 34.184 196 99 11 23 208 13 188 3.49e-11 65.1
MS.gene062537.t1 MTR_1g107385 24.528 477 259 22 9 445 5 420 6.53e-11 64.3
MS.gene062537.t1 MTR_3g105060 27.778 198 131 7 23 217 14 202 8.65e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062537.t1 AT1G78750 26.955 486 271 24 13 445 1 455 2.13e-20 94.0
MS.gene062537.t1 AT1G78750 26.955 486 271 24 13 445 1 455 2.13e-20 94.0
MS.gene062537.t1 AT5G56420 26.009 446 281 18 23 448 6 422 1.66e-18 87.8
MS.gene062537.t1 AT5G56420 26.009 446 281 18 23 448 6 422 1.66e-18 87.8
MS.gene062537.t1 AT5G56420 26.009 446 281 18 23 448 6 422 1.66e-18 87.8
MS.gene062537.t1 AT1G32375 27.617 449 274 18 23 448 2 422 3.33e-18 87.0
MS.gene062537.t1 AT1G32375 27.617 449 274 18 23 448 2 422 3.33e-18 87.0
MS.gene062537.t1 AT1G13780 25.977 435 258 18 23 415 10 422 1.93e-17 84.7
MS.gene062537.t1 AT5G53635 26.872 454 271 18 25 447 2 425 1.95e-17 84.7
MS.gene062537.t1 AT4G26340 27.912 455 262 17 23 448 2 419 1.50e-16 82.0
MS.gene062537.t1 AT1G78760 24.895 474 290 20 13 449 1 445 3.37e-16 80.9
MS.gene062537.t1 AT4G13985 24.380 484 285 21 10 445 6 456 1.17e-15 79.3
MS.gene062537.t1 AT1G66290 24.628 471 285 19 9 445 16 450 1.65e-15 79.0
MS.gene062537.t1 AT1G51370 25.734 443 276 19 23 443 19 430 1.96e-15 78.6
MS.gene062537.t1 AT5G56440 27.174 460 268 18 23 447 2 429 3.30e-15 77.8
MS.gene062537.t1 AT5G18770 25.490 459 285 16 23 446 24 460 1.47e-14 76.3
MS.gene062537.t1 AT1G16930 25.847 472 269 21 22 448 14 449 5.07e-14 74.3
MS.gene062537.t1 AT1G16930 25.847 472 269 21 22 448 14 449 5.07e-14 74.3
MS.gene062537.t1 AT1G16930 25.847 472 269 21 22 448 14 449 5.07e-14 74.3
MS.gene062537.t1 AT1G66320 26.160 474 274 21 11 445 18 454 5.55e-14 74.3
MS.gene062537.t1 AT4G09920 31.959 194 115 7 23 210 2 184 6.60e-14 72.4
MS.gene062537.t1 AT4G09920 31.959 194 115 7 23 210 2 184 6.60e-14 72.4
MS.gene062537.t1 AT4G09920 31.959 194 115 7 23 210 2 184 6.60e-14 72.4
MS.gene062537.t1 AT4G09920 31.959 194 115 7 23 210 2 184 7.10e-14 72.8
MS.gene062537.t1 AT4G09920 31.959 194 115 7 23 210 2 184 7.10e-14 72.8
MS.gene062537.t1 AT1G69630 26.068 468 279 20 10 440 6 443 7.18e-14 73.9
MS.gene062537.t1 AT5G53840 25.435 460 296 18 9 445 2 437 8.46e-14 73.6
MS.gene062537.t1 AT2G26030 26.022 465 277 21 23 445 4 443 1.28e-13 73.2
MS.gene062537.t1 AT3G59200 32.558 215 119 11 23 224 7 208 1.97e-13 72.0
MS.gene062537.t1 AT5G27750 25.055 451 274 16 23 427 5 437 3.21e-13 71.6
MS.gene062537.t1 AT3G59200 32.558 215 119 11 23 224 7 208 3.28e-13 72.0
MS.gene062537.t1 AT2G26030 26.395 466 269 23 23 445 4 438 3.74e-13 71.6
MS.gene062537.t1 AT2G26030 26.395 466 269 23 23 445 4 438 3.74e-13 71.6
MS.gene062537.t1 AT5G22720 26.471 476 265 21 23 447 23 464 6.31e-13 70.9
MS.gene062537.t1 AT1G13780 30.455 220 119 11 23 224 10 213 8.66e-13 69.7
MS.gene062537.t1 AT4G15060 26.303 422 238 19 7 394 11 393 1.10e-12 70.1
MS.gene062537.t1 AT5G44960 28.337 427 222 21 23 413 5 383 1.19e-12 69.7
MS.gene062537.t1 AT5G50270 26.056 426 249 12 23 415 2 394 1.60e-12 69.3
MS.gene062537.t1 AT5G44950 25.108 462 266 19 23 439 4 430 2.22e-12 68.9
MS.gene062537.t1 AT1G55660 25.708 459 293 20 20 447 50 491 2.32e-12 69.3
MS.gene062537.t1 AT3G58940 30.806 211 110 9 23 210 2 199 2.59e-12 69.3
MS.gene062537.t1 AT3G58940 30.806 211 110 9 23 210 2 199 2.59e-12 69.3
MS.gene062537.t1 AT3G49030 27.354 446 283 21 23 450 21 443 1.13e-11 67.0
MS.gene062537.t1 AT1G51370 30.928 194 111 8 23 211 19 194 1.67e-11 65.9
MS.gene062537.t1 AT1G51370 30.928 194 111 8 23 211 19 194 2.79e-11 65.5
MS.gene062537.t1 AT1G49610 27.536 207 113 6 11 195 6 197 3.36e-11 65.1
MS.gene062537.t1 AT5G53640 29.665 209 131 8 8 213 6 201 4.00e-11 63.2
MS.gene062537.t1 AT3G26922 27.170 265 171 10 14 274 7 253 4.10e-11 64.3
MS.gene062537.t1 AT3G26922 27.170 265 171 10 14 274 7 253 4.10e-11 64.3
MS.gene062537.t1 AT5G35995 29.289 239 134 9 23 254 5 215 5.09e-11 65.1
MS.gene062537.t1 AT5G35995 29.289 239 134 9 23 254 5 215 5.09e-11 65.1
MS.gene062537.t1 AT5G56810 28.139 462 272 23 12 445 2 431 6.48e-11 64.7

Find 109 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATTTCTTCTTCTGCTTT+TGG 0.217119 8.1:+44430572 None:intergenic
CCACTCAACTTCGTTCAAAA+AGG 0.237370 8.1:-44430378 MS.gene062537:CDS
GCCAACTCATGCCCCTGTTC+TGG 0.268203 8.1:-44429467 MS.gene062537:CDS
TGATGTAAATCGCGAAATTC+AGG 0.289569 8.1:+44429439 None:intergenic
CATTGTTGCGACGGAAGTTC+TGG 0.309789 8.1:+44430466 None:intergenic
GACCCAGTTGTATCTTCTTA+AGG 0.337539 8.1:-44428929 None:intergenic
TGTTGGTAACTTTCACAGTA+TGG 0.341967 8.1:-44429735 MS.gene062537:CDS
TTCTAATACTGCAGTCTTCA+CGG 0.375556 8.1:-44430028 MS.gene062537:intron
CTCGGATGAGGTTGAGAATA+GGG 0.385948 8.1:+44429663 None:intergenic
TCAAGGACTTGAAGATGAAT+TGG 0.403742 8.1:-44429101 MS.gene062537:CDS
TATCTCTGGCTGCCCTAGGC+TGG 0.405016 8.1:-44429891 MS.gene062537:CDS
GAATTAGTTTCCCTTATCTC+TGG 0.405102 8.1:-44429905 MS.gene062537:CDS
ATGCTTCAACTCAAATCTTC+TGG 0.414841 8.1:-44429398 MS.gene062537:CDS
CTGACCTATGTTCACATAGA+AGG 0.414878 8.1:-44429127 MS.gene062537:CDS
TCACCTTAAGAAGATACAAC+TGG 0.418927 8.1:+44428926 None:intergenic
CCTAGGGCAGCCAGAGATAA+GGG 0.428957 8.1:+44429895 None:intergenic
GAAAGTCGAAGTCGAAGATC+TGG 0.433372 8.1:+44430402 None:intergenic
CTCCTAGATATCTTCATCTC+TGG 0.433430 8.1:-44430433 MS.gene062537:CDS
CTTCAGTGATGGAAAATAAA+CGG 0.433476 8.1:+44429964 None:intergenic
CTCTTAATCGTTTGAGAGAA+CGG 0.434146 8.1:+44428990 None:intergenic
TTGCGACGGAAGTTCTGGTT+GGG 0.435631 8.1:+44430471 None:intergenic
AATTCAGGATAATCCAGAAC+AGG 0.435810 8.1:+44429454 None:intergenic
TTCGAATGTCGCGGGATTTG+CGG 0.439604 8.1:+44430324 None:intergenic
CCCTTATCTCTGGCTGCCCT+AGG 0.444881 8.1:-44429895 MS.gene062537:CDS
TTGGGAGGAAGGACATGATA+TGG 0.449100 8.1:+44430489 None:intergenic
CGCTTCTGTCATCTCGGATG+AGG 0.454440 8.1:+44429651 None:intergenic
GTCTCTCGGCGAAGATATTG+TGG 0.456589 8.1:-44429936 MS.gene062537:CDS
ACTTACTTTAATATAAATGA+AGG 0.456907 8.1:-44429776 MS.gene062537:CDS
TTCCAGAGATGAAGATATCT+AGG 0.456977 8.1:+44430431 None:intergenic
AGTCCAAGTGAGATCGTCAT+TGG 0.462467 8.1:+44429796 None:intergenic
GAAGAAATTGACGGCGGAGG+AGG 0.465548 8.1:-44430560 MS.gene062537:CDS
GGAAGGTTTCTCGAGAACTT+TGG 0.472309 8.1:+44429820 None:intergenic
ACCATTGAGAACATGGCAAC+CGG 0.478521 8.1:-44429185 MS.gene062537:CDS
CAACTGGGTCAAATTTAAGT+TGG 0.482494 8.1:+44428942 None:intergenic
CCTCCAATGACGATCTCACT+TGG 0.486462 8.1:-44429799 MS.gene062537:CDS
GCCTAGGGCAGCCAGAGATA+AGG 0.486530 8.1:+44429894 None:intergenic
AAGATAGAGTTGTTGAAGGT+GGG 0.486842 8.1:+44430214 None:intergenic
ACATGCAAAGTTTCCAGCCT+AGG 0.487752 8.1:+44429878 None:intergenic
TCTCGGATGAGGTTGAGAAT+AGG 0.495111 8.1:+44429662 None:intergenic
TGCCATGTTCTCAATGGTAG+TGG 0.499626 8.1:+44429190 None:intergenic
GATTTGAGTTGAAGCATGGA+AGG 0.506598 8.1:+44429404 None:intergenic
ACGTGGATTCTTGCTGCCAT+TGG 0.513966 8.1:-44430239 MS.gene062537:CDS
TTGTTGAAGGTGGGGTCCAA+TGG 0.515637 8.1:+44430223 None:intergenic
GCACTAACCTCCTTTCCCTC+AGG 0.516291 8.1:-44430142 MS.gene062537:intron
GGAAGTTCTGGTTGGGAGGA+AGG 0.519935 8.1:+44430478 None:intergenic
GTTCACATAGAAGGATATCA+AGG 0.520078 8.1:-44429118 MS.gene062537:CDS
CTTCTATGTGAACATAGGTC+AGG 0.522459 8.1:+44429128 None:intergenic
GAGGCGATGACCTCACCGAC+AGG 0.523086 8.1:-44430541 MS.gene062537:CDS
GAAATTCGCTTCTGTCATCT+CGG 0.525450 8.1:+44429645 None:intergenic
TACAAAGATAGAGTTGTTGA+AGG 0.530392 8.1:+44430210 None:intergenic
GTTGCGACGGAAGTTCTGGT+TGG 0.533913 8.1:+44430470 None:intergenic
AAAGATAGAGTTGTTGAAGG+TGG 0.535343 8.1:+44430213 None:intergenic
TCAGAAACTGTTATTGACTC+CGG 0.543380 8.1:+44429166 None:intergenic
CATGCAAAGTTTCCAGCCTA+GGG 0.543778 8.1:+44429879 None:intergenic
ATATCCTTCTATGTGAACAT+AGG 0.544669 8.1:+44429123 None:intergenic
ATAAGGGAAACTAATTCAGT+TGG 0.544720 8.1:+44429911 None:intergenic
CGCTGAATCCACGATGGCGG+AGG 0.546316 8.1:-44430603 None:intergenic
GAAGAAGAAATTGACGGCGG+AGG 0.547881 8.1:-44430563 MS.gene062537:CDS
CCAAGTGAGATCGTCATTGG+AGG 0.548795 8.1:+44429799 None:intergenic
TCCGGTTGCCATGTTCTCAA+TGG 0.550198 8.1:+44429184 None:intergenic
GCAAAAGTTACCTGAGGGAA+AGG 0.550738 8.1:+44430132 None:intergenic
ATAGATCCTCATCATCGTCT+AGG 0.550888 8.1:+44429612 None:intergenic
AGAAGATTTGAGTTGAAGCA+TGG 0.552646 8.1:+44429400 None:intergenic
GAAAATAAACGGAGTTTGCA+CGG 0.554058 8.1:+44429975 None:intergenic
CACCTTAAGAAGATACAACT+GGG 0.558337 8.1:+44428927 None:intergenic
ATATGGCATAGTACAGAGTC+CGG 0.561225 8.1:+44430506 None:intergenic
AACCACTACCATTGAGAACA+TGG 0.563418 8.1:-44429192 MS.gene062537:CDS
TCCAGAACAGGGGCATGAGT+TGG 0.570030 8.1:+44429466 None:intergenic
CGGTAGAGTGCTCAGCCTGT+CGG 0.570238 8.1:+44430526 None:intergenic
CGACAGGCTGAGCACTCTAC+CGG 0.570912 8.1:-44430525 MS.gene062537:CDS
TGGCACATGTCAGAGCCCCT+TGG 0.573859 8.1:+44428962 None:intergenic
TACAAATTCAGTTTCAGCCA+CGG 0.574682 8.1:+44429688 None:intergenic
GCAAATCCCGCGACATTCGA+AGG 0.578368 8.1:-44430322 MS.gene062537:CDS
TCTCTCGGCGAAGATATTGT+GGG 0.579344 8.1:-44429935 MS.gene062537:CDS
AGATAGAGTTGTTGAAGGTG+GGG 0.580102 8.1:+44430215 None:intergenic
TCTTCACGGTTCAATCGAAA+CGG 0.582481 8.1:-44430014 MS.gene062537:CDS
ACGGAACCTTCGAATGTCGC+GGG 0.584834 8.1:+44430316 None:intergenic
CTATGTGAACATAGGTCAGG+TGG 0.588925 8.1:+44429131 None:intergenic
GACGAGACTCATTGTTGCGA+CGG 0.590038 8.1:+44430457 None:intergenic
AGAGACAATATCTTCAGTGA+TGG 0.590680 8.1:+44429953 None:intergenic
CGACGGAAGTTCTGGTTGGG+AGG 0.592790 8.1:+44430474 None:intergenic
GTGAGATCGTCATTGGAGGA+AGG 0.596129 8.1:+44429803 None:intergenic
AAAGCAGAAGAAGAAATTGA+CGG 0.596467 8.1:-44430569 MS.gene062537:CDS
CTGAGTTGTAACTCATCGCG+TGG 0.606212 8.1:+44430272 None:intergenic
GACAGAAGCGAATTTCATAA+CGG 0.611584 8.1:-44429638 MS.gene062537:CDS
GATCCTCATCATCGTCTAGG+AGG 0.620289 8.1:+44429615 None:intergenic
GAGTGCTCAGCCTGTCGGTG+AGG 0.620743 8.1:+44430531 None:intergenic
TCACTGAAGATATTGTCTCT+CGG 0.622255 8.1:-44429950 MS.gene062537:CDS
TAGATATCTTCATCTCTGGA+AGG 0.622526 8.1:-44430429 MS.gene062537:CDS
CGGCCTCCTAGACGATGATG+AGG 0.623457 8.1:-44429618 MS.gene062537:CDS
GTTGCGTCATTCGACATCAA+AGG 0.623983 8.1:-44429582 MS.gene062537:intron
CAATTGATGAAGAGTGAACG+AGG 0.626927 8.1:+44430164 None:intergenic
AAAGTTACCTGAGGGAAAGG+AGG 0.628632 8.1:+44430135 None:intergenic
GACGGAACCTTCGAATGTCG+CGG 0.632919 8.1:+44430315 None:intergenic
TGGTAACTTTCACAGTATGG+TGG 0.637517 8.1:-44429732 MS.gene062537:CDS
ATTCAGGATAATCCAGAACA+GGG 0.639651 8.1:+44429455 None:intergenic
TATGTGAACATAGGTCAGGT+GGG 0.643030 8.1:+44429132 None:intergenic
TCTCATAATTCAGAGCAACA+AGG 0.643040 8.1:-44429341 MS.gene062537:intron
CATCAAATGTGAGAGTGAGA+CGG 0.650223 8.1:+44430297 None:intergenic
AACGATTAAGAGACAAACCA+AGG 0.651423 8.1:-44428979 MS.gene062537:CDS
AACTCAGGAATGCTGAAACG+TGG 0.660563 8.1:-44430256 MS.gene062537:CDS
ACGATTAAGAGACAAACCAA+GGG 0.664610 8.1:-44428978 MS.gene062537:CDS
TTCAATGAACAAAACAGACA+AGG 0.679515 8.1:-44429014 MS.gene062537:CDS
CACGCGATGAGTTACAACTC+AGG 0.685874 8.1:-44430271 MS.gene062537:CDS
TTATGAGAACTTGAAGCATG+TGG 0.695487 8.1:+44429356 None:intergenic
CGATTAAGAGACAAACCAAG+GGG 0.721773 8.1:-44428977 MS.gene062537:CDS
TTCAGGATAATCCAGAACAG+GGG 0.721899 8.1:+44429456 None:intergenic
GCAGAAGAAGAAATTGACGG+CGG 0.742332 8.1:-44430566 MS.gene062537:CDS
AATTGATGAAGAGTGAACGA+GGG 0.761342 8.1:+44430165 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAATTCAAACATTCTT+TGG - chr8.1:44430218-44430237 MS.gene062537:CDS 15.0%
!! ACTTACTTTAATATAAATGA+AGG - chr8.1:44429745-44429764 MS.gene062537:CDS 15.0%
!!! AAATGAAGGATGTTTTAAAT+TGG - chr8.1:44429759-44429778 MS.gene062537:CDS 20.0%
!!! AATGAAGGATGTTTTAAATT+GGG - chr8.1:44429760-44429779 MS.gene062537:CDS 20.0%
! TAATTCAAACATTCTTTGGT+AGG - chr8.1:44430222-44430241 MS.gene062537:CDS 25.0%
!! CATTCGTTGAATATATTTTG+AGG - chr8.1:44430443-44430462 MS.gene062537:CDS 25.0%
!!! ACACATATATTTTTGAGTCA+CGG - chr8.1:44430278-44430297 MS.gene062537:CDS 25.0%
!!! ATGTTTTTACATGTGTAGTT+TGG - chr8.1:44429986-44430005 MS.gene062537:CDS 25.0%
!!! GTTGAATATATTTTGAGGAA+CGG - chr8.1:44430448-44430467 MS.gene062537:CDS 25.0%
AAAGCAGAAGAAGAAATTGA+CGG - chr8.1:44428952-44428971 MS.gene062537:CDS 30.0%
ATAAGGGAAACTAATTCAGT+TGG + chr8.1:44429613-44429632 None:intergenic 30.0%
ATATCCTTCTATGTGAACAT+AGG + chr8.1:44430401-44430420 None:intergenic 30.0%
TACAAAGATAGAGTTGTTGA+AGG + chr8.1:44429314-44429333 None:intergenic 30.0%
TTCAATGAACAAAACAGACA+AGG - chr8.1:44430507-44430526 MS.gene062537:CDS 30.0%
! CTTCAGTGATGGAAAATAAA+CGG + chr8.1:44429560-44429579 None:intergenic 30.0%
! TCAATTTCTTCTTCTGCTTT+TGG + chr8.1:44428952-44428971 None:intergenic 30.0%
!! TGTTTTAAATTGGGCATTGT+TGG - chr8.1:44429769-44429788 MS.gene062537:CDS 30.0%
!!! TATATTTTTGAGTCACGGTA+TGG - chr8.1:44430283-44430302 MS.gene062537:CDS 30.0%
AAAGATAGAGTTGTTGAAGG+TGG + chr8.1:44429311-44429330 None:intergenic 35.0%
AACGATTAAGAGACAAACCA+AGG - chr8.1:44430542-44430561 MS.gene062537:CDS 35.0%
AAGATAGAGTTGTTGAAGGT+GGG + chr8.1:44429310-44429329 None:intergenic 35.0%
AATTCAGGATAATCCAGAAC+AGG + chr8.1:44430070-44430089 None:intergenic 35.0%
AATTGATGAAGAGTGAACGA+GGG + chr8.1:44429359-44429378 None:intergenic 35.0%
ACGATTAAGAGACAAACCAA+GGG - chr8.1:44430543-44430562 MS.gene062537:CDS 35.0%
AGAGACAATATCTTCAGTGA+TGG + chr8.1:44429571-44429590 None:intergenic 35.0%
ATGCTTCAACTCAAATCTTC+TGG - chr8.1:44430123-44430142 MS.gene062537:intron 35.0%
ATTCAGGATAATCCAGAACA+GGG + chr8.1:44430069-44430088 None:intergenic 35.0%
CAACTGGGTCAAATTTAAGT+TGG + chr8.1:44430582-44430601 None:intergenic 35.0%
CTCTTAATCGTTTGAGAGAA+CGG + chr8.1:44430534-44430553 None:intergenic 35.0%
GAAAATAAACGGAGTTTGCA+CGG + chr8.1:44429549-44429568 None:intergenic 35.0%
GACAGAAGCGAATTTCATAA+CGG - chr8.1:44429883-44429902 MS.gene062537:CDS 35.0%
GTTCACATAGAAGGATATCA+AGG - chr8.1:44430403-44430422 MS.gene062537:CDS 35.0%
TACAAATTCAGTTTCAGCCA+CGG + chr8.1:44429836-44429855 None:intergenic 35.0%
TACACGATAACAACAAGTGA+TGG + chr8.1:44430025-44430044 None:intergenic 35.0%
TAGATATCTTCATCTCTGGA+AGG - chr8.1:44429092-44429111 MS.gene062537:CDS 35.0%
TCAAGGACTTGAAGATGAAT+TGG - chr8.1:44430420-44430439 MS.gene062537:CDS 35.0%
TCACTGAAGATATTGTCTCT+CGG - chr8.1:44429571-44429590 MS.gene062537:intron 35.0%
TCTCATAATTCAGAGCAACA+AGG - chr8.1:44430180-44430199 MS.gene062537:CDS 35.0%
TGATGTAAATCGCGAAATTC+AGG + chr8.1:44430085-44430104 None:intergenic 35.0%
TTATGAGAACTTGAAGCATG+TGG + chr8.1:44430168-44430187 None:intergenic 35.0%
TTCCAGAGATGAAGATATCT+AGG + chr8.1:44429093-44429112 None:intergenic 35.0%
TTCTAATACTGCAGTCTTCA+CGG - chr8.1:44429493-44429512 MS.gene062537:intron 35.0%
! GAATTAGTTTCCCTTATCTC+TGG - chr8.1:44429616-44429635 MS.gene062537:CDS 35.0%
!! AGAAGATTTGAGTTGAAGCA+TGG + chr8.1:44430124-44430143 None:intergenic 35.0%
!! ATTTCTTCTTCTGCTTTTGG+TGG + chr8.1:44428949-44428968 None:intergenic 35.0%
!! TCAGAAACTGTTATTGACTC+CGG + chr8.1:44430358-44430377 None:intergenic 35.0%
!! TGTTGGTAACTTTCACAGTA+TGG - chr8.1:44429786-44429805 MS.gene062537:CDS 35.0%
AACCACTACCATTGAGAACA+TGG - chr8.1:44430329-44430348 MS.gene062537:CDS 40.0%
AGATAGAGTTGTTGAAGGTG+GGG + chr8.1:44429309-44429328 None:intergenic 40.0%
ATAGATCCTCATCATCGTCT+AGG + chr8.1:44429912-44429931 None:intergenic 40.0%
ATATGGCATAGTACAGAGTC+CGG + chr8.1:44429018-44429037 None:intergenic 40.0%
CAATTGATGAAGAGTGAACG+AGG + chr8.1:44429360-44429379 None:intergenic 40.0%
CATCAAATGTGAGAGTGAGA+CGG + chr8.1:44429227-44429246 None:intergenic 40.0%
CATCTGCAAAAGTTACCTGA+GGG + chr8.1:44429397-44429416 None:intergenic 40.0%
CCACTCAACTTCGTTCAAAA+AGG - chr8.1:44429143-44429162 MS.gene062537:CDS 40.0%
CGATTAAGAGACAAACCAAG+GGG - chr8.1:44430544-44430563 MS.gene062537:CDS 40.0%
CTCCTAGATATCTTCATCTC+TGG - chr8.1:44429088-44429107 MS.gene062537:CDS 40.0%
CTGACCTATGTTCACATAGA+AGG - chr8.1:44430394-44430413 MS.gene062537:CDS 40.0%
CTTCTATGTGAACATAGGTC+AGG + chr8.1:44430396-44430415 None:intergenic 40.0%
GAAATTCGCTTCTGTCATCT+CGG + chr8.1:44429879-44429898 None:intergenic 40.0%
TATGTGAACATAGGTCAGGT+GGG + chr8.1:44430392-44430411 None:intergenic 40.0%
TCATCTGCAAAAGTTACCTG+AGG + chr8.1:44429398-44429417 None:intergenic 40.0%
TCTTCACGGTTCAATCGAAA+CGG - chr8.1:44429507-44429526 MS.gene062537:intron 40.0%
TGGTAACTTTCACAGTATGG+TGG - chr8.1:44429789-44429808 MS.gene062537:CDS 40.0%
TTCAGGATAATCCAGAACAG+GGG + chr8.1:44430068-44430087 None:intergenic 40.0%
! CCTTTTTGAACGAAGTTGAG+TGG + chr8.1:44429146-44429165 None:intergenic 40.0%
! GTTTCTTGATGTCTTTTCCG+TGG - chr8.1:44429816-44429835 MS.gene062537:CDS 40.0%
!! GATTTGAGTTGAAGCATGGA+AGG + chr8.1:44430120-44430139 None:intergenic 40.0%
AAAGTTACCTGAGGGAAAGG+AGG + chr8.1:44429389-44429408 None:intergenic 45.0%
AACTCAGGAATGCTGAAACG+TGG - chr8.1:44429265-44429284 MS.gene062537:intron 45.0%
ACATGCAAAGTTTCCAGCCT+AGG + chr8.1:44429646-44429665 None:intergenic 45.0%
ACCATTGAGAACATGGCAAC+CGG - chr8.1:44430336-44430355 MS.gene062537:CDS 45.0%
AGTCCAAGTGAGATCGTCAT+TGG + chr8.1:44429728-44429747 None:intergenic 45.0%
CATGCAAAGTTTCCAGCCTA+GGG + chr8.1:44429645-44429664 None:intergenic 45.0%
CTATGTGAACATAGGTCAGG+TGG + chr8.1:44430393-44430412 None:intergenic 45.0%
CTCGGATGAGGTTGAGAATA+GGG + chr8.1:44429861-44429880 None:intergenic 45.0%
GAAAGTCGAAGTCGAAGATC+TGG + chr8.1:44429122-44429141 None:intergenic 45.0%
GCAAAAGTTACCTGAGGGAA+AGG + chr8.1:44429392-44429411 None:intergenic 45.0%
GCAGAAGAAGAAATTGACGG+CGG - chr8.1:44428955-44428974 MS.gene062537:CDS 45.0%
GGAAGGTTTCTCGAGAACTT+TGG + chr8.1:44429704-44429723 None:intergenic 45.0%
GTTCCTCCTACAACCAACAA+GGG + chr8.1:44430312-44430331 None:intergenic 45.0%
GTTGCGTCATTCGACATCAA+AGG - chr8.1:44429939-44429958 MS.gene062537:CDS 45.0%
TCTCGGATGAGGTTGAGAAT+AGG + chr8.1:44429862-44429881 None:intergenic 45.0%
TCTCTCGGCGAAGATATTGT+GGG - chr8.1:44429586-44429605 MS.gene062537:CDS 45.0%
TGCCATGTTCTCAATGGTAG+TGG + chr8.1:44430334-44430353 None:intergenic 45.0%
TTGGGAGGAAGGACATGATA+TGG + chr8.1:44429035-44429054 None:intergenic 45.0%
TTGTTATCGTGTACGTGCAG+TGG - chr8.1:44430032-44430051 MS.gene062537:intron 45.0%
ACGTGGATTCTTGCTGCCAT+TGG - chr8.1:44429282-44429301 MS.gene062537:intron 50.0%
CACGCGATGAGTTACAACTC+AGG - chr8.1:44429250-44429269 MS.gene062537:intron 50.0%
CATTGTTGCGACGGAAGTTC+TGG + chr8.1:44429058-44429077 None:intergenic 50.0%
CCAAGTGAGATCGTCATTGG+AGG + chr8.1:44429725-44429744 None:intergenic 50.0%
CCTCCAATGACGATCTCACT+TGG - chr8.1:44429722-44429741 MS.gene062537:CDS 50.0%
CTGAGTTGTAACTCATCGCG+TGG + chr8.1:44429252-44429271 None:intergenic 50.0%
GAAGAAGAAATTGACGGCGG+AGG - chr8.1:44428958-44428977 MS.gene062537:CDS 50.0%
GACGAGACTCATTGTTGCGA+CGG + chr8.1:44429067-44429086 None:intergenic 50.0%
GATCCTCATCATCGTCTAGG+AGG + chr8.1:44429909-44429928 None:intergenic 50.0%
GGTTCCTCCTACAACCAACA+AGG + chr8.1:44430313-44430332 None:intergenic 50.0%
GTCTCTCGGCGAAGATATTG+TGG - chr8.1:44429585-44429604 MS.gene062537:CDS 50.0%
GTGAGATCGTCATTGGAGGA+AGG + chr8.1:44429721-44429740 None:intergenic 50.0%
TCCGGTTGCCATGTTCTCAA+TGG + chr8.1:44430340-44430359 None:intergenic 50.0%
TTCGAATGTCGCGGGATTTG+CGG + chr8.1:44429200-44429219 None:intergenic 50.0%
TTGTTGAAGGTGGGGTCCAA+TGG + chr8.1:44429301-44429320 None:intergenic 50.0%
! TGGTGTCCCTTGTTGGTTGT+AGG - chr8.1:44430303-44430322 MS.gene062537:CDS 50.0%
! TGTCCCTTGTTGGTTGTAGG+AGG - chr8.1:44430306-44430325 MS.gene062537:CDS 50.0%
! TTGCGACGGAAGTTCTGGTT+GGG + chr8.1:44429053-44429072 None:intergenic 50.0%
ACGGAACCTTCGAATGTCGC+GGG + chr8.1:44429208-44429227 None:intergenic 55.0%
CACGGTATGGTGTCCCTTGT+TGG - chr8.1:44430296-44430315 MS.gene062537:CDS 55.0%
CCTAGGGCAGCCAGAGATAA+GGG + chr8.1:44429629-44429648 None:intergenic 55.0%
CGCTTCTGTCATCTCGGATG+AGG + chr8.1:44429873-44429892 None:intergenic 55.0%
GAAGAAATTGACGGCGGAGG+AGG - chr8.1:44428961-44428980 MS.gene062537:CDS 55.0%
GACGGAACCTTCGAATGTCG+CGG + chr8.1:44429209-44429228 None:intergenic 55.0%
GCAAATCCCGCGACATTCGA+AGG - chr8.1:44429199-44429218 MS.gene062537:CDS 55.0%
GCACTAACCTCCTTTCCCTC+AGG - chr8.1:44429379-44429398 MS.gene062537:CDS 55.0%
! GGAAGTTCTGGTTGGGAGGA+AGG + chr8.1:44429046-44429065 None:intergenic 55.0%
! GTTGCGACGGAAGTTCTGGT+TGG + chr8.1:44429054-44429073 None:intergenic 55.0%
! TCCAGAACAGGGGCATGAGT+TGG + chr8.1:44430058-44430077 None:intergenic 55.0%
CCCTTATCTCTGGCTGCCCT+AGG - chr8.1:44429626-44429645 MS.gene062537:CDS 60.0%
CGACAGGCTGAGCACTCTAC+CGG - chr8.1:44428996-44429015 MS.gene062537:CDS 60.0%
CGGCCTCCTAGACGATGATG+AGG - chr8.1:44429903-44429922 MS.gene062537:CDS 60.0%
GCCAACTCATGCCCCTGTTC+TGG - chr8.1:44430054-44430073 MS.gene062537:intron 60.0%
GCCTAGGGCAGCCAGAGATA+AGG + chr8.1:44429630-44429649 None:intergenic 60.0%
TATCTCTGGCTGCCCTAGGC+TGG - chr8.1:44429630-44429649 MS.gene062537:CDS 60.0%
TGGCACATGTCAGAGCCCCT+TGG + chr8.1:44430562-44430581 None:intergenic 60.0%
! CGACGGAAGTTCTGGTTGGG+AGG + chr8.1:44429050-44429069 None:intergenic 60.0%
! CGGTAGAGTGCTCAGCCTGT+CGG + chr8.1:44428998-44429017 None:intergenic 60.0%
GAGGCGATGACCTCACCGAC+AGG - chr8.1:44428980-44428999 MS.gene062537:CDS 65.0%
GAGTGCTCAGCCTGTCGGTG+AGG + chr8.1:44428993-44429012 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 44428931 44430612 44428931 ID=MS.gene062537
chr8.1 mRNA 44428931 44430612 44428931 ID=MS.gene062537.t1;Parent=MS.gene062537
chr8.1 exon 44430143 44430612 44430143 ID=MS.gene062537.t1.exon1;Parent=MS.gene062537.t1
chr8.1 CDS 44430143 44430612 44430143 ID=cds.MS.gene062537.t1;Parent=MS.gene062537.t1
chr8.1 exon 44429583 44430036 44429583 ID=MS.gene062537.t1.exon2;Parent=MS.gene062537.t1
chr8.1 CDS 44429583 44430036 44429583 ID=cds.MS.gene062537.t1;Parent=MS.gene062537.t1
chr8.1 exon 44429342 44429491 44429342 ID=MS.gene062537.t1.exon3;Parent=MS.gene062537.t1
chr8.1 CDS 44429342 44429491 44429342 ID=cds.MS.gene062537.t1;Parent=MS.gene062537.t1
chr8.1 exon 44428931 44429218 44428931 ID=MS.gene062537.t1.exon4;Parent=MS.gene062537.t1
chr8.1 CDS 44428931 44429218 44428931 ID=cds.MS.gene062537.t1;Parent=MS.gene062537.t1
Gene Sequence

>MS.gene062537

ATGGCGGAGGAGTCACCACCAAAAGCAGAAGAAGAAATTGACGGCGGAGGAGGCGATGACCTCACCGACAGGCTGAGCACTCTACCGGACTCTGTACTATGCCATATCATGTCCTTCCTCCCAACCAGAACTTCCGTCGCAACAATGAGTCTCGTCTCTCCTAGATATCTTCATCTCTGGAAGGATCTCCAGATCTTCGACTTCGACTTTCACCACTCAACTTCGTTCAAAAAGGTTGCATTATTCATTAACTCTGTTCTCACTCTCCGCAAATCCCGCGACATTCGAAGGTTCCGTCTCACTCTCACATTTGATGATCCACGCGATGAGTTACAACTCAGGAATGCTGAAACGTGGATTCTTGCTGCCATTGGACCCCACCTTCAACAACTCTATCTTTGTATTGACGAGTCTTGTATTGACATCCCTCGTTCACTCTTCATCAATTGCACTAACCTCCTTTCCCTCAGGTAACTTTTGCAGATGATACTCATGTTTTAATGAAAACTCAGAATGATTTGTTTTTATGTTCAAACTCACTTTTACTTTCGAATCATATTTTTTCTAATACTGCAGTCTTCACGGTTCAATCGAAACGGCACACTGCAATCTTGACCGTGCAAACTCCGTTTATTTTCCATCACTGAAGATATTGTCTCTCGGCGAAGATATTGTGGGTCCAACTGAATTAGTTTCCCTTATCTCTGGCTGCCCTAGGCTGGAAACTTTGCATGTTTACTTTTACATTGTCTCTGAAGAAATGTCTTTCACCAAAGTTCTCGAGAAACCTTCCTCCAATGACGATCTCACTTGGACTTACTTTAATATAAATGAAGGATGTTTTAAATTGGGCATTGTTGGTAACTTTCACAGTATGGTGGAAGCGTTTCTTGATGTCTTTTCCGTGGCTGAAACTGAATTTGTAGACCCTATTCTCAACCTCATCCGAGATGACAGAAGCGAATTTCATAACGGCCTCCTAGACGATGATGAGGATCTATATCTAGAGTTGCGTCATTCGACATCAAAGGTGAAGTTTTACTTTTATGCTTTATATGTTTTTACATGTGTAGTTTGGTTCTAATATTGCGCCATCACTTGTTGTTATCGTGTACGTGCAGTGGCCAACTCATGCCCCTGTTCTGGATTATCCTGAATTTCGCGATTTACATCATCTGAAGTTCATCCTTCCATGCTTCAACTCAAATCTTCTGGTAAATGTTCTTGAGCAATGCCACATGCTTCAAGTTCTCATAATTCAGAGCAACAAGGTTTGTTGAATTTTGAAATTTAATTCAAACATTCTTTGGTAGGTCTATATATACATGCATGTATGTCTCTATCTGTACACATATATTTTTGAGTCACGGTATGGTGTCCCTTGTTGGTTGTAGGAGGAACCACTACCATTGAGAACATGGCAACCGGAGTCAATAACAGTTTCTGAATGTCTCAAATCCCACCTGACCTATGTTCACATAGAAGGATATCAAGGACTTGAAGATGAATTGGCATTCGTTGAATATATTTTGAGGAACGGAGTTGTTTTGAAGACAATGCTTATTTTTGTTGATATTTCAATGAACAAAACAGACAAGGACCGTTCTCTCAAACGATTAAGAGACAAACCAAGGGGCTCTGACATGTGCCAACTTAAATTTGACCCAGTTGTATCTTCTTAA
Protein sequence

>MS.gene062537.t1

MAEESPPKAEEEIDGGGGDDLTDRLSTLPDSVLCHIMSFLPTRTSVATMSLVSPRYLHLWKDLQIFDFDFHHSTSFKKVALFINSVLTLRKSRDIRRFRLTLTFDDPRDELQLRNAETWILAAIGPHLQQLYLCIDESCIDIPRSLFINCTNLLSLSLHGSIETAHCNLDRANSVYFPSLKILSLGEDIVGPTELVSLISGCPRLETLHVYFYIVSEEMSFTKVLEKPSSNDDLTWTYFNINEGCFKLGIVGNFHSMVEAFLDVFSVAETEFVDPILNLIRDDRSEFHNGLLDDDEDLYLELRHSTSKWPTHAPVLDYPEFRDLHHLKFILPCFNSNLLVNVLEQCHMLQVLIIQSNKEEPLPLRTWQPESITVSECLKSHLTYVHIEGYQGLEDELAFVEYILRNGVVLKTMLIFVDISMNKTDKDRSLKRLRDKPRGSDMCQLKFDPVVSS