AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34707

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34707.t1 MTR_8g062300 53.988 489 160 13 4 436 3 482 1.04e-154 447
MS.gene34707.t1 MTR_8g070950 53.548 465 159 13 1 435 1 438 4.15e-146 423
MS.gene34707.t1 MTR_8g062080 50.422 474 173 13 1 436 1 450 1.30e-137 402
MS.gene34707.t1 MTR_8g062010 54.072 442 159 13 22 435 9 434 4.65e-135 395
MS.gene34707.t1 MTR_8g062310 49.443 449 183 9 6 435 9 432 2.25e-134 393
MS.gene34707.t1 MTR_8g058965 46.450 493 175 14 1 436 1 461 5.07e-123 365
MS.gene34707.t1 MTR_8g469020 46.072 471 181 13 19 435 4 455 1.71e-121 361
MS.gene34707.t1 MTR_2g086020 55.013 389 104 8 48 435 1 319 4.73e-121 355
MS.gene34707.t1 MTR_8g467790 49.333 375 144 9 21 376 147 494 1.89e-101 311
MS.gene34707.t1 MTR_4g050250 43.387 431 164 14 9 377 8 420 9.00e-92 284
MS.gene34707.t1 MTR_2g083840 36.632 475 228 17 6 431 7 457 3.40e-70 229
MS.gene34707.t1 MTR_8g468810 35.611 483 239 16 1 431 1 463 8.54e-64 213
MS.gene34707.t1 MTR_8g014260 35.491 479 233 22 1 431 1 451 8.12e-63 210
MS.gene34707.t1 MTR_8g058970 32.922 486 259 16 3 431 2 477 2.27e-59 201
MS.gene34707.t1 MTR_8g467800 33.684 475 251 16 6 435 2 457 4.45e-59 200
MS.gene34707.t1 MTR_8g077460 35.765 425 199 16 22 431 10 375 1.51e-58 196
MS.gene34707.t1 MTR_4g032730 32.120 467 246 11 21 430 14 466 2.18e-58 198
MS.gene34707.t1 MTR_8g062270 33.550 462 232 20 21 430 15 453 4.80e-58 197
MS.gene34707.t1 MTR_8g468760 34.744 449 228 19 15 430 2 418 6.18e-55 188
MS.gene34707.t1 MTR_5g092960 32.680 459 251 10 18 430 15 461 2.90e-49 174
MS.gene34707.t1 MTR_8g445780 35.646 418 207 17 1 384 1 390 1.73e-48 170
MS.gene34707.t1 MTR_8g014160 34.813 428 218 18 22 431 41 425 3.83e-48 170
MS.gene34707.t1 MTR_8g468840 32.347 473 251 18 1 429 1 448 3.11e-47 168
MS.gene34707.t1 MTR_8g468810 34.527 391 186 15 1 341 1 371 2.00e-39 145
MS.gene34707.t1 MTR_8g468810 40.260 231 106 9 1 213 1 217 1.43e-34 131
MS.gene34707.t1 MTR_8g468810 40.260 231 106 9 1 213 1 217 1.44e-34 131
MS.gene34707.t1 MTR_8g468810 40.260 231 106 9 1 213 1 217 2.09e-34 131
MS.gene34707.t1 MTR_7g098510 28.959 442 252 16 1 431 1 391 3.11e-32 126
MS.gene34707.t1 MTR_4g024710 33.117 308 164 12 130 431 76 347 1.11e-31 124
MS.gene34707.t1 MTR_1g041245 30.734 436 222 21 19 431 8 386 4.51e-31 123
MS.gene34707.t1 MTR_8g467800 42.396 217 103 10 6 213 2 205 2.73e-30 120
MS.gene34707.t1 MTR_7g032380 29.176 425 213 18 19 431 8 356 2.86e-29 117
MS.gene34707.t1 MTR_5g092960 32.514 366 196 8 18 341 15 371 3.44e-29 118
MS.gene34707.t1 MTR_8g014210 43.709 151 81 4 282 431 84 231 2.66e-28 112
MS.gene34707.t1 MTR_3g099800 63.218 87 31 1 343 428 19 105 3.71e-28 107
MS.gene34707.t1 MTR_7g115270 29.724 434 238 18 19 428 9 399 8.93e-27 111
MS.gene34707.t1 MTR_1089s0010 27.635 427 239 17 21 431 7 379 2.21e-25 107
MS.gene34707.t1 MTR_7g098480 27.740 447 247 16 1 430 1 388 4.07e-24 104
MS.gene34707.t1 MTR_4g024745 31.752 274 156 10 167 414 16 284 6.09e-24 101
MS.gene34707.t1 MTR_5g073420 27.563 439 236 18 13 430 2 379 8.60e-24 102
MS.gene34707.t1 MTR_7g098570 27.876 452 229 16 5 426 4 388 9.87e-24 103
MS.gene34707.t1 MTR_3g108150 29.078 423 243 17 19 430 10 386 6.53e-23 100
MS.gene34707.t1 MTR_5g008950 26.714 423 237 17 24 431 1 365 1.40e-22 99.0
MS.gene34707.t1 MTR_3g107100 26.450 431 253 18 22 431 11 398 1.75e-22 99.4
MS.gene34707.t1 MTR_7g056433 27.064 436 234 16 22 431 12 389 2.37e-22 98.6
MS.gene34707.t1 MTR_7g034115 28.066 424 230 16 19 423 8 375 2.49e-22 98.6
MS.gene34707.t1 MTR_5g021550 40.341 176 74 10 1 159 1 162 4.54e-22 92.8
MS.gene34707.t1 MTR_8g014290 37.430 179 74 8 1 160 1 160 1.37e-20 88.6
MS.gene34707.t1 MTR_2g007450 29.540 457 247 21 7 428 1 417 1.40e-20 94.0
MS.gene34707.t1 MTR_3g105060 26.179 424 241 18 19 431 11 373 1.72e-20 92.8
MS.gene34707.t1 MTR_7g032370 27.027 407 232 15 34 431 2 352 2.92e-20 92.0
MS.gene34707.t1 MTR_2g008310 28.538 424 237 18 21 431 1 371 3.48e-20 92.4
MS.gene34707.t1 MTR_3g107160 25.472 424 260 14 18 431 6 383 5.48e-20 91.7
MS.gene34707.t1 MTR_6g040270 27.681 401 215 19 24 409 10 350 7.49e-20 91.3
MS.gene34707.t1 MTR_7g033990 26.128 421 242 17 20 424 9 376 8.81e-20 91.3
MS.gene34707.t1 MTR_7g007080 28.333 420 239 19 22 424 4 378 2.69e-19 89.7
MS.gene34707.t1 MTR_7g056420 26.277 411 232 14 22 423 9 357 3.96e-19 90.9
MS.gene34707.t1 MTR_7g056430 26.277 411 232 14 22 423 9 357 4.82e-19 90.5
MS.gene34707.t1 MTR_1g108820 29.245 424 247 18 16 428 2 383 1.22e-18 87.8
MS.gene34707.t1 MTR_3g036250 26.098 410 251 10 21 428 4 363 4.49e-18 85.9
MS.gene34707.t1 MTR_3g099830 27.540 443 224 23 11 428 1 371 7.66e-18 85.1
MS.gene34707.t1 MTR_5g084070 26.818 440 271 18 12 423 3 419 9.06e-18 85.5
MS.gene34707.t1 MTR_3g107120 26.147 436 256 17 22 435 9 400 1.26e-17 84.7
MS.gene34707.t1 MTR_7g026500 24.312 436 260 12 16 430 8 394 1.39e-17 84.7
MS.gene34707.t1 MTR_5g080710 29.257 417 238 20 21 403 32 425 2.47e-17 84.3
MS.gene34707.t1 MTR_1g085120 25.814 430 249 17 22 431 92 471 2.80e-17 84.3
MS.gene34707.t1 MTR_7g056447 35.533 197 108 9 18 210 5 186 2.82e-17 82.4
MS.gene34707.t1 MTR_5g058100 25.734 443 241 18 15 430 2 383 3.13e-17 83.6
MS.gene34707.t1 MTR_8g014230 42.857 84 46 2 341 424 17 98 3.46e-17 77.4
MS.gene34707.t1 MTR_1g085120 25.814 430 249 17 22 431 167 546 5.23e-17 83.6
MS.gene34707.t1 MTR_5g083890 26.555 418 231 19 18 401 2 377 5.37e-17 83.6
MS.gene34707.t1 MTR_7g055943 27.107 439 236 24 21 431 39 421 1.20e-16 82.0
MS.gene34707.t1 MTR_4g093690 31.915 235 127 12 3 225 2 215 1.20e-16 80.1
MS.gene34707.t1 MTR_6g022360 23.684 418 261 13 20 430 41 407 6.72e-16 79.7
MS.gene34707.t1 MTR_6g011460 29.535 430 237 20 18 431 42 421 7.10e-16 79.7
MS.gene34707.t1 MTR_5g058000 26.056 426 241 19 21 430 7 374 1.04e-15 79.0
MS.gene34707.t1 MTR_2g007500 28.726 369 208 19 20 378 12 335 1.88e-15 77.4
MS.gene34707.t1 MTR_5g061680 26.722 363 185 14 13 365 2 293 2.00e-15 77.4
MS.gene34707.t1 MTR_8g085430 32.558 215 115 8 13 213 2 200 5.55e-15 76.3
MS.gene34707.t1 MTR_4g039440 25.858 437 243 17 20 431 32 412 8.16e-15 76.3
MS.gene34707.t1 MTR_3g071850 26.031 388 239 13 21 396 23 374 9.51e-15 77.0
MS.gene34707.t1 MTR_6g044560 24.729 461 236 15 15 431 171 564 1.06e-14 76.6
MS.gene34707.t1 MTR_3g108560 26.190 420 248 18 22 430 20 388 1.94e-14 75.1
MS.gene34707.t1 MTR_6g022500 24.714 437 245 19 20 431 99 476 2.56e-14 75.1
MS.gene34707.t1 MTR_7g088260 26.730 419 251 15 22 431 91 462 3.53e-14 74.7
MS.gene34707.t1 MTR_6g044572 24.835 455 231 15 21 431 1 388 6.90e-14 73.6
MS.gene34707.t1 MTR_1g053025 26.777 422 259 17 21 428 1 386 7.16e-14 73.2
MS.gene34707.t1 MTR_4g056020 32.057 209 105 9 22 214 9 196 2.02e-13 72.4
MS.gene34707.t1 MTR_4g024720 39.231 130 62 6 9 130 7 127 2.07e-13 69.3
MS.gene34707.t1 MTR_7g075820 24.146 439 254 20 19 428 3 391 4.09e-13 70.9
MS.gene34707.t1 MTR_1g095750 41.667 84 47 2 19 101 43 125 6.53e-13 66.2
MS.gene34707.t1 MTR_7g034110 45.679 81 41 2 19 99 8 85 7.12e-13 70.1
MS.gene34707.t1 MTR_4g024770 52.564 78 29 4 1 78 8 77 1.54e-12 63.2
MS.gene34707.t1 MTR_3g026920 24.550 444 254 16 20 434 17 408 2.13e-12 68.9
MS.gene34707.t1 MTR_2g005990 25.057 435 254 18 20 428 4 392 3.26e-12 68.2
MS.gene34707.t1 MTR_0009s0080 24.658 438 257 16 1 417 1 386 2.83e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34707.t1 AT1G32375 26.697 442 273 16 21 431 1 422 7.44e-26 109
MS.gene34707.t1 AT1G32375 26.697 442 273 16 21 431 1 422 7.44e-26 109
MS.gene34707.t1 AT4G26340 27.815 453 251 17 21 431 1 419 5.72e-21 95.1
MS.gene34707.t1 AT4G13965 27.294 436 265 17 21 428 9 420 3.32e-20 92.8
MS.gene34707.t1 AT5G60610 25.060 415 239 16 21 395 1 383 4.27e-18 86.3
MS.gene34707.t1 AT5G60610 25.060 415 239 16 21 395 1 383 4.27e-18 86.3
MS.gene34707.t1 AT4G10400 25.581 430 278 15 21 430 1 408 2.33e-17 84.0
MS.gene34707.t1 AT4G10400 25.581 430 278 15 21 430 1 408 2.33e-17 84.0
MS.gene34707.t1 AT4G10400 25.581 430 278 15 21 430 1 408 2.33e-17 84.0
MS.gene34707.t1 AT4G00160 27.093 454 268 18 22 435 16 446 6.96e-16 79.7
MS.gene34707.t1 AT3G26922 32.323 198 113 7 22 214 14 195 1.77e-15 77.4
MS.gene34707.t1 AT3G26922 32.323 198 113 7 22 214 14 195 1.77e-15 77.4
MS.gene34707.t1 AT1G78760 24.895 478 265 21 9 432 8 445 1.08e-14 76.3
MS.gene34707.t1 AT5G56560 26.637 443 261 20 23 431 6 418 1.34e-14 75.9
MS.gene34707.t1 AT1G55030 24.760 416 253 18 8 389 1 390 3.40e-14 74.7
MS.gene34707.t1 AT1G55030 24.750 400 245 17 20 389 7 380 4.60e-14 73.9
MS.gene34707.t1 AT4G15060 31.373 204 112 8 21 213 25 211 8.94e-14 73.2
MS.gene34707.t1 AT5G56440 33.333 201 105 10 21 211 1 182 1.12e-13 73.2
MS.gene34707.t1 AT4G15060 31.373 204 112 8 21 213 171 357 1.19e-13 73.2
MS.gene34707.t1 AT1G78750 24.684 474 278 20 9 428 7 455 2.17e-13 72.4
MS.gene34707.t1 AT1G78750 24.684 474 278 20 9 428 7 455 2.17e-13 72.4
MS.gene34707.t1 AT2G26860 25.616 406 248 13 21 390 1 388 2.48e-13 71.6
MS.gene34707.t1 AT2G26860 25.616 406 248 13 21 390 1 388 2.48e-13 71.6
MS.gene34707.t1 AT5G50270 26.037 434 254 18 21 413 1 408 3.15e-13 71.6
MS.gene34707.t1 AT5G22670 25.408 429 231 18 22 394 11 406 5.12e-13 70.9
MS.gene34707.t1 AT5G38590 27.765 443 247 17 21 424 1 409 6.82e-13 70.5
MS.gene34707.t1 AT5G56400 24.832 447 277 17 21 431 32 455 8.91e-13 70.5
MS.gene34707.t1 AT1G13780 24.094 469 274 18 19 428 7 452 1.37e-12 69.7
MS.gene34707.t1 AT3G58940 28.372 215 116 9 21 213 1 199 1.62e-12 69.7
MS.gene34707.t1 AT3G58940 28.372 215 116 9 21 213 1 199 1.62e-12 69.7
MS.gene34707.t1 AT4G00315 31.343 201 117 9 21 213 1 188 3.94e-12 68.2
MS.gene34707.t1 AT5G22700 26.500 400 244 15 22 404 43 409 4.02e-12 68.2
MS.gene34707.t1 AT1G69630 23.789 475 280 16 1 421 1 447 6.05e-12 67.8
MS.gene34707.t1 AT1G16930 30.000 200 113 7 22 213 15 195 1.05e-11 67.0
MS.gene34707.t1 AT1G16930 30.000 200 113 7 22 213 15 195 1.05e-11 67.0
MS.gene34707.t1 AT1G16930 30.000 200 113 7 22 213 15 195 1.05e-11 67.0
MS.gene34707.t1 AT5G35995 28.319 226 123 7 22 233 5 205 1.19e-11 66.6
MS.gene34707.t1 AT5G35995 28.319 226 123 7 22 233 5 205 1.19e-11 66.6
MS.gene34707.t1 AT5G22700 31.020 245 143 9 22 261 6 229 1.73e-11 66.2
MS.gene34707.t1 AT5G22700 31.020 245 143 9 22 261 6 229 1.73e-11 66.2
MS.gene34707.t1 AT5G22700 31.020 245 143 9 22 261 6 229 1.73e-11 66.2
MS.gene34707.t1 AT5G22700 31.020 245 143 9 22 261 6 229 1.73e-11 66.2
MS.gene34707.t1 AT5G22700 31.020 245 143 9 22 261 43 266 2.09e-11 66.2
MS.gene34707.t1 AT5G53840 25.178 421 245 17 22 401 18 409 3.22e-11 65.5
MS.gene34707.t1 AT1G78730 26.425 193 112 5 21 213 21 183 5.09e-11 64.7
MS.gene34707.t1 AT1G13780 29.018 224 128 10 19 228 7 213 6.94e-11 63.9

Find 93 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCAGGCTGGAGACTTTGC+AGG 0.207215 8.1:+45631415 MS.gene34707:CDS
GATCCACGCTTCAGCATTCT+TGG 0.265206 8.1:-45631039 None:intergenic
TTTGCGGAACGGACTTGTTT+TGG 0.289001 8.1:+45632263 MS.gene34707:CDS
CAACTTTATCTCAATGTTGA+TGG 0.302931 8.1:+45631089 MS.gene34707:CDS
TGATGTAAATCGCGAAATTC+AGG 0.303964 8.1:-45631884 None:intergenic
GAAATTCACTTCTGGTATCT+TGG 0.314508 8.1:-45631675 None:intergenic
CGCCTTTGATCGCCTTCAAA+GGG 0.315839 8.1:+45630992 MS.gene34707:CDS
GACCCAGATGTATCTTCTTA+AGG 0.337539 8.1:+45632396 MS.gene34707:CDS
TCATCTCTGGAAGGATCTTC+AGG 0.350215 8.1:+45630857 MS.gene34707:CDS
CAAGAACACAACCCCTTTGA+AGG 0.357310 8.1:-45631004 None:intergenic
ATGTTTCAACTCAAATCTTC+TGG 0.358687 8.1:+45631925 MS.gene34707:CDS
TCTCGCTGGCTGCCCCAGGC+TGG 0.373730 8.1:+45631403 MS.gene34707:CDS
TTCTAATACTGCAGTCTTCA+CGG 0.375556 8.1:+45631266 MS.gene34707:intron
GCTTCTATGAAATTCACTTC+TGG 0.378368 8.1:-45631683 None:intergenic
TTACTAATGATGAAACTGTT+TGG 0.390635 8.1:-45630914 None:intergenic
TCACCTTAAGAAGATACATC+TGG 0.406097 8.1:-45632399 None:intergenic
CATCCTCATAGTTAATTATG+AGG 0.414466 8.1:-45631705 None:intergenic
CTGCTCAACCTGTCGACGAC+TGG 0.430154 8.1:-45630744 None:intergenic
TGGGGAGGAAGGACATGATA+TGG 0.433088 8.1:-45630788 None:intergenic
CTGACTTATATTCACATAGA+AGG 0.433553 8.1:+45632198 MS.gene34707:CDS
CTTCAGTGATGGAAAATAAA+CGG 0.436856 8.1:-45631330 None:intergenic
TCTTTCTCGCTGGCTGCCCC+AGG 0.439455 8.1:+45631399 MS.gene34707:CDS
ACCTGCAAAGTCTCCAGCCT+GGG 0.440076 8.1:-45631416 None:intergenic
TTTGAATGTTGCGGGATTTG+CGG 0.447214 8.1:-45630953 None:intergenic
TCGCCTTTGATCGCCTTCAA+AGG 0.447806 8.1:+45630991 MS.gene34707:CDS
CTCGCAGGTATCTTCATCTC+TGG 0.455203 8.1:+45630844 MS.gene34707:CDS
AATTCAGGATAATTTAGAAC+AGG 0.463615 8.1:-45631869 None:intergenic
GAGATAAAGTTGTTGAAGGT+GGG 0.465366 8.1:-45631078 None:intergenic
CACTTCTGGTATCTTGGATG+AGG 0.469527 8.1:-45631669 None:intergenic
CATCTGGGTCAAATTTAAGT+TGG 0.478361 8.1:-45632383 None:intergenic
GGAAGGTGTCACTAGAACTT+TGG 0.483436 8.1:-45631477 None:intergenic
ACATGTCATCATCGGAAGAA+AGG 0.484670 8.1:-45631448 None:intergenic
TGCTCAACCTGTCGACGACT+GGG 0.488561 8.1:-45630743 None:intergenic
CTTCTATGTGAATATAAGTC+AGG 0.489932 8.1:-45632197 None:intergenic
TGAGATAAAGTTGTTGAAGG+TGG 0.491370 8.1:-45631079 None:intergenic
CATTGAGATAAAGTTGTTGA+AGG 0.494116 8.1:-45631082 None:intergenic
GCACCAACCTCGTTTCCCTC+AGG 0.500754 8.1:+45631147 MS.gene34707:CDS
AGTTACTTTAATATAAATGA+AGG 0.511759 8.1:+45631545 MS.gene34707:CDS
TCCGGCTGCCATGTTCTCAA+TGG 0.513864 8.1:-45632141 None:intergenic
TTGTTGAAGGTGGGGTCCAA+TGG 0.515637 8.1:-45631069 None:intergenic
ATTCACATAGAAGGATATCA+AGG 0.522151 8.1:+45632207 MS.gene34707:CDS
GCGTGGATCCTTGCTGCCAT+TGG 0.523474 8.1:+45631053 MS.gene34707:CDS
ATTTATATTAAAGTAACTCC+AGG 0.524406 8.1:-45631540 None:intergenic
AAGCCTCATAATTAACTATG+AGG 0.525772 8.1:+45631702 MS.gene34707:CDS
CACCTTAAGAAGATACATCT+GGG 0.528482 8.1:-45632398 None:intergenic
AATGCGACCCAGTCGTCGAC+AGG 0.531434 8.1:+45630736 MS.gene34707:CDS
AACCTGCAAAGTCTCCAGCC+TGG 0.539126 8.1:-45631417 None:intergenic
ACCCCTTTGAAGGCGATCAA+AGG 0.543073 8.1:-45630994 None:intergenic
CGATGAGCCTTGTCTCTCGC+AGG 0.545430 8.1:+45630829 MS.gene34707:CDS
TTTAGAACAGGTGCATGAGT+TGG 0.547228 8.1:-45631857 None:intergenic
CGTCGATGGCGGAGGAGTCA+AGG 0.548687 8.1:+45630685 None:intergenic
AACAACCACCATTGAGAACA+TGG 0.552420 8.1:+45632133 MS.gene34707:CDS
GGATATCAAGCTGTTCGAGC+TGG 0.558732 8.1:-45631501 None:intergenic
AGATAAAGTTGTTGAAGGTG+GGG 0.559902 8.1:-45631077 None:intergenic
CCGCTGATGACAATTTCACC+TGG 0.562804 8.1:+45631522 MS.gene34707:CDS
ACCATTGAGAACATGGCAGC+CGG 0.564288 8.1:+45632140 MS.gene34707:CDS
TGAATCGACGTCGATGGCGG+AGG 0.566196 8.1:+45630677 None:intergenic
ATATGGCATAACAACGAGTC+CGG 0.568277 8.1:-45630771 None:intergenic
AAGCTGTTCGAGCTGGAGGA+AGG 0.570594 8.1:-45631494 None:intergenic
CGACAGGTTGAGCAGTCTAC+CGG 0.574240 8.1:+45630752 MS.gene34707:CDS
GAATGTTGCGGGATTTGCGG+AGG 0.575959 8.1:-45630950 None:intergenic
GTCTCTCGGCAATGATATTG+TGG 0.576609 8.1:+45631358 MS.gene34707:CDS
GGCTGCCATGTTCTCAATGG+TGG 0.577603 8.1:-45632138 None:intergenic
GGTCAAAGACATGTCATCAT+CGG 0.578513 8.1:-45631456 None:intergenic
TGTGGCATTGCTCAAGACAA+TGG 0.586966 8.1:-45631949 None:intergenic
AGAAGATTTGAGTTGAAACA+TGG 0.586977 8.1:-45631923 None:intergenic
GACAAGGCTCATCGTAGTAA+CGG 0.589535 8.1:-45630820 None:intergenic
AGAGACAACATCTTCAGTGA+TGG 0.590452 8.1:-45631341 None:intergenic
TCAGAAACTCTTGTTGACTC+CGG 0.591556 8.1:-45632159 None:intergenic
AAGATTAACAAATGTAACGA+AGG 0.592044 8.1:+45632347 MS.gene34707:CDS
GATTTGAGTTGAAACATGGA+AGG 0.600986 8.1:-45631919 None:intergenic
TATCAAGCTGTTCGAGCTGG+AGG 0.602132 8.1:-45631498 None:intergenic
AATGATGAAACTGTTTGGAA+CGG 0.605943 8.1:-45630909 None:intergenic
TCACTGAAGATGTTGTCTCT+CGG 0.609046 8.1:+45631344 MS.gene34707:CDS
CAGGTATCTTCATCTCTGGA+AGG 0.616499 8.1:+45630848 MS.gene34707:CDS
CTATGTGAATATAAGTCAGG+TGG 0.618165 8.1:-45632194 None:intergenic
GTTGCATCATTCGATGTCAA+AGG 0.618438 8.1:+45631738 MS.gene34707:CDS
TTACCTGAGGGAAACGAGGT+TGG 0.622908 8.1:-45631150 None:intergenic
GGTGGGGTCCAATGGCAGCA+AGG 0.624675 8.1:-45631061 None:intergenic
GATCAAAGGCGAAAGTGAGA+TGG 0.632265 8.1:-45630980 None:intergenic
GAAGATACCTGCGAGAGACA+AGG 0.633083 8.1:-45630836 None:intergenic
CAATTGATAAAGAGTGAACG+AGG 0.640528 8.1:-45631125 None:intergenic
AGATTAACAAATGTAACGAA+GGG 0.641585 8.1:+45632348 MS.gene34707:CDS
TCTCATAATTCAGAGCAACA+AGG 0.643040 8.1:+45631982 MS.gene34707:CDS
CCAGGTGAAATTGTCATCAG+CGG 0.651525 8.1:-45631522 None:intergenic
TATGTGAATATAAGTCAGGT+GGG 0.652099 8.1:-45632193 None:intergenic
AATCCAAGAATGCTGAAGCG+TGG 0.654776 8.1:+45631036 MS.gene34707:CDS
GCCTTTGATCGCCTTCAAAG+GGG 0.664858 8.1:+45630993 MS.gene34707:CDS
CCTGCAAAGTCTCCAGCCTG+GGG 0.668022 8.1:-45631415 None:intergenic
CTGTTTGGAACGGAATTGTG+TGG 0.688147 8.1:-45630899 None:intergenic
TTATGAGAACTTGAAGCATG+TGG 0.695487 8.1:-45631967 None:intergenic
GAAGTTACCTGAGGGAAACG+AGG 0.701057 8.1:-45631154 None:intergenic
AATTGATAAAGAGTGAACGA+GGG 0.746792 8.1:-45631124 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATATTTGTGTACAAA+TGG - chr8.1:45632075-45632094 None:intergenic 15.0%
!! AATTTAATTCAAACATTCTT+TGG + chr8.1:45632020-45632039 MS.gene34707:intron 15.0%
!!! AGTTACTTTAATATAAATGA+AGG + chr8.1:45631545-45631564 MS.gene34707:CDS 15.0%
!! ATTTATATTAAAGTAACTCC+AGG - chr8.1:45631543-45631562 None:intergenic 20.0%
!!! ACAAATATATTTTTGAGTCA+CGG + chr8.1:45632082-45632101 MS.gene34707:intron 20.0%
!!! TGTTTTTGTTGATATTTCAA+TGG + chr8.1:45632296-45632315 MS.gene34707:CDS 20.0%
!!! TTGTTAATCTTTTGATAGAA+CGG - chr8.1:45632338-45632357 None:intergenic 20.0%
! AAGATTAACAAATGTAACGA+AGG + chr8.1:45632347-45632366 MS.gene34707:CDS 25.0%
! AGATTAACAAATGTAACGAA+GGG + chr8.1:45632348-45632367 MS.gene34707:CDS 25.0%
! ATGTGTTTAAATGTGTACTT+TGG + chr8.1:45631785-45631804 MS.gene34707:intron 25.0%
! TAATTCAAACATTCTTTGGT+CGG + chr8.1:45632024-45632043 MS.gene34707:intron 25.0%
! TTACTAATGATGAAACTGTT+TGG - chr8.1:45630917-45630936 None:intergenic 25.0%
!! AATTCAGGATAATTTAGAAC+AGG - chr8.1:45631872-45631891 None:intergenic 25.0%
!!! AAATGAAGGACGTTTTAAAT+TGG + chr8.1:45631559-45631578 MS.gene34707:intron 25.0%
!!! AAGGTGAAGTTTTACTTTTA+TGG + chr8.1:45631757-45631776 MS.gene34707:intron 25.0%
!!! AATGAAGGACGTTTTAAATT+GGG + chr8.1:45631560-45631579 MS.gene34707:intron 25.0%
!!! CATTTGCTGAATATATTTTG+CGG + chr8.1:45632247-45632266 MS.gene34707:CDS 25.0%
AAGCCTCATAATTAACTATG+AGG + chr8.1:45631702-45631721 MS.gene34707:CDS 30.0%
AATGATGAAACTGTTTGGAA+CGG - chr8.1:45630912-45630931 None:intergenic 30.0%
AATTGATAAAGAGTGAACGA+GGG - chr8.1:45631127-45631146 None:intergenic 30.0%
ATGTTTCAACTCAAATCTTC+TGG + chr8.1:45631925-45631944 MS.gene34707:CDS 30.0%
ATTCACATAGAAGGATATCA+AGG + chr8.1:45632207-45632226 MS.gene34707:CDS 30.0%
CAACTTTATCTCAATGTTGA+TGG + chr8.1:45631089-45631108 MS.gene34707:CDS 30.0%
CATCCTCATAGTTAATTATG+AGG - chr8.1:45631708-45631727 None:intergenic 30.0%
CATTGAGATAAAGTTGTTGA+AGG - chr8.1:45631085-45631104 None:intergenic 30.0%
CTGACTTATATTCACATAGA+AGG + chr8.1:45632198-45632217 MS.gene34707:CDS 30.0%
CTTCTATGTGAATATAAGTC+AGG - chr8.1:45632200-45632219 None:intergenic 30.0%
TAAAAACAGAAGTTACCTGA+GGG - chr8.1:45631165-45631184 None:intergenic 30.0%
TATGTGAATATAAGTCAGGT+GGG - chr8.1:45632196-45632215 None:intergenic 30.0%
TTAAAAACAGAAGTTACCTG+AGG - chr8.1:45631166-45631185 None:intergenic 30.0%
TTTAATCCCATCTTAATTGC+AGG + chr8.1:45631193-45631212 MS.gene34707:intron 30.0%
! CTTCAGTGATGGAAAATAAA+CGG - chr8.1:45631333-45631352 None:intergenic 30.0%
! TTCAAAAGTGAAAGTGTGTT+TGG - chr8.1:45631237-45631256 None:intergenic 30.0%
!! AGAAGATTTGAGTTGAAACA+TGG - chr8.1:45631926-45631945 None:intergenic 30.0%
!! GTAGTAACGGAAATTTTTGT+GGG - chr8.1:45630810-45630829 None:intergenic 30.0%
!! TAGTAACGGAAATTTTTGTG+GGG - chr8.1:45630809-45630828 None:intergenic 30.0%
!!! CATTTCTTGATGTTTTTTCC+CGG + chr8.1:45631615-45631634 MS.gene34707:intron 30.0%
AGATAAAGTTGTTGAAGGTG+GGG - chr8.1:45631080-45631099 None:intergenic 35.0%
CAATTGATAAAGAGTGAACG+AGG - chr8.1:45631128-45631147 None:intergenic 35.0%
CATCTGGGTCAAATTTAAGT+TGG - chr8.1:45632386-45632405 None:intergenic 35.0%
CTATGTGAATATAAGTCAGG+TGG - chr8.1:45632197-45632216 None:intergenic 35.0%
GAAATTCACTTCTGGTATCT+TGG - chr8.1:45631678-45631697 None:intergenic 35.0%
GAGATAAAGTTGTTGAAGGT+GGG - chr8.1:45631081-45631100 None:intergenic 35.0%
GAGTATCCTGCAATTAAGAT+GGG - chr8.1:45631202-45631221 None:intergenic 35.0%
GCTTCTATGAAATTCACTTC+TGG - chr8.1:45631686-45631705 None:intergenic 35.0%
TCTACAAATTCACTTTCAGC+CGG - chr8.1:45631637-45631656 None:intergenic 35.0%
TCTCATAATTCAGAGCAACA+AGG + chr8.1:45631982-45632001 MS.gene34707:CDS 35.0%
TGAGATAAAGTTGTTGAAGG+TGG - chr8.1:45631082-45631101 None:intergenic 35.0%
TGAGTATCCTGCAATTAAGA+TGG - chr8.1:45631203-45631222 None:intergenic 35.0%
TGATGTAAATCGCGAAATTC+AGG - chr8.1:45631887-45631906 None:intergenic 35.0%
TGGTAACTTTCGTAATATGG+TGG + chr8.1:45631589-45631608 MS.gene34707:intron 35.0%
TTATGAGAACTTGAAGCATG+TGG - chr8.1:45631970-45631989 None:intergenic 35.0%
TTCAGAGCAACAAGGTTTAT+TGG + chr8.1:45631990-45632009 MS.gene34707:intron 35.0%
TTCTAATACTGCAGTCTTCA+CGG + chr8.1:45631266-45631285 MS.gene34707:intron 35.0%
! GCTGAATATATTTTGCGGAA+CGG + chr8.1:45632252-45632271 MS.gene34707:CDS 35.0%
!! CGTAGTAACGGAAATTTTTG+TGG - chr8.1:45630811-45630830 None:intergenic 35.0%
!! CGTTGGTAACTTTCGTAATA+TGG + chr8.1:45631586-45631605 MS.gene34707:intron 35.0%
!! GATTTGAGTTGAAACATGGA+AGG - chr8.1:45631922-45631941 None:intergenic 35.0%
!!! ATGGAAGTTTTGAATGTTGC+GGG - chr8.1:45630964-45630983 None:intergenic 35.0%
!!! GAATTAGTTTTCTTTCTCGC+TGG + chr8.1:45631389-45631408 MS.gene34707:CDS 35.0%
!!! GATGGAAGTTTTGAATGTTG+CGG - chr8.1:45630965-45630984 None:intergenic 35.0%
!!! TATATTTTTGAGTCACGGCT+TGG + chr8.1:45632087-45632106 MS.gene34707:intron 35.0%
AACAACCACCATTGAGAACA+TGG + chr8.1:45632133-45632152 MS.gene34707:CDS 40.0%
ACATGTCATCATCGGAAGAA+AGG - chr8.1:45631451-45631470 None:intergenic 40.0%
AGAGACAACATCTTCAGTGA+TGG - chr8.1:45631344-45631363 None:intergenic 40.0%
ATATGGCATAACAACGAGTC+CGG - chr8.1:45630774-45630793 None:intergenic 40.0%
CTACAAATTCACTTTCAGCC+GGG - chr8.1:45631636-45631655 None:intergenic 40.0%
GGTCAAAGACATGTCATCAT+CGG - chr8.1:45631459-45631478 None:intergenic 40.0%
GTTGCATCATTCGATGTCAA+AGG + chr8.1:45631738-45631757 MS.gene34707:CDS 40.0%
TCACTGAAGATGTTGTCTCT+CGG + chr8.1:45631344-45631363 MS.gene34707:CDS 40.0%
TTGTTATCGTGTATGTGCAG+TGG + chr8.1:45631834-45631853 MS.gene34707:intron 40.0%
TTTGAATGTTGCGGGATTTG+CGG - chr8.1:45630956-45630975 None:intergenic 40.0%
! TAACGGAAATTTTTGTGGGG+AGG - chr8.1:45630806-45630825 None:intergenic 40.0%
! TTTAGAACAGGTGCATGAGT+TGG - chr8.1:45631860-45631879 None:intergenic 40.0%
!! CGTTTTAAATTGGGCATCGT+TGG + chr8.1:45631569-45631588 MS.gene34707:intron 40.0%
!! TCAGAAACTCTTGTTGACTC+CGG - chr8.1:45632162-45632181 None:intergenic 40.0%
AATCCAAGAATGCTGAAGCG+TGG + chr8.1:45631036-45631055 MS.gene34707:CDS 45.0%
CAAGAACACAACCCCTTTGA+AGG - chr8.1:45631007-45631026 None:intergenic 45.0%
CACTTCTGGTATCTTGGATG+AGG - chr8.1:45631672-45631691 None:intergenic 45.0%
CAGGTATCTTCATCTCTGGA+AGG + chr8.1:45630848-45630867 MS.gene34707:CDS 45.0%
CCAGGTGAAATTGTCATCAG+CGG - chr8.1:45631525-45631544 None:intergenic 45.0%
GACAAGGCTCATCGTAGTAA+CGG - chr8.1:45630823-45630842 None:intergenic 45.0%
GATCAAAGGCGAAAGTGAGA+TGG - chr8.1:45630983-45631002 None:intergenic 45.0%
GTCTCTCGGCAATGATATTG+TGG + chr8.1:45631358-45631377 MS.gene34707:CDS 45.0%
TGTGGCATTGCTCAAGACAA+TGG - chr8.1:45631952-45631971 None:intergenic 45.0%
! CTGTTTGGAACGGAATTGTG+TGG - chr8.1:45630902-45630921 None:intergenic 45.0%
! GGAAATTTTTGTGGGGAGGA+AGG - chr8.1:45630802-45630821 None:intergenic 45.0%
! TCATCTCTGGAAGGATCTTC+AGG + chr8.1:45630857-45630876 MS.gene34707:CDS 45.0%
!! GGAAGGTGTCACTAGAACTT+TGG - chr8.1:45631480-45631499 None:intergenic 45.0%
!! TTTGCGGAACGGACTTGTTT+TGG + chr8.1:45632263-45632282 MS.gene34707:CDS 45.0%
ACCATTGAGAACATGGCAGC+CGG + chr8.1:45632140-45632159 MS.gene34707:CDS 50.0%
CCGCTGATGACAATTTCACC+TGG + chr8.1:45631522-45631541 MS.gene34707:CDS 50.0%
CGCCTTTGATCGCCTTCAAA+GGG + chr8.1:45630992-45631011 MS.gene34707:CDS 50.0%
CTCGCAGGTATCTTCATCTC+TGG + chr8.1:45630844-45630863 MS.gene34707:CDS 50.0%
GAAGATACCTGCGAGAGACA+AGG - chr8.1:45630839-45630858 None:intergenic 50.0%
GAAGTTACCTGAGGGAAACG+AGG - chr8.1:45631157-45631176 None:intergenic 50.0%
GATCCACGCTTCAGCATTCT+TGG - chr8.1:45631042-45631061 None:intergenic 50.0%
GCCTTTGATCGCCTTCAAAG+GGG + chr8.1:45630993-45631012 MS.gene34707:CDS 50.0%
GGATATCAAGCTGTTCGAGC+TGG - chr8.1:45631504-45631523 None:intergenic 50.0%
GTTCCTCCTACGAGCAACAA+GGG - chr8.1:45632116-45632135 None:intergenic 50.0%
TATCAAGCTGTTCGAGCTGG+AGG - chr8.1:45631501-45631520 None:intergenic 50.0%
TCGCCTTTGATCGCCTTCAA+AGG + chr8.1:45630991-45631010 MS.gene34707:CDS 50.0%
TGGGGAGGAAGGACATGATA+TGG - chr8.1:45630791-45630810 None:intergenic 50.0%
TGTTCCTCCTACGAGCAACA+AGG - chr8.1:45632117-45632136 None:intergenic 50.0%
TTACCTGAGGGAAACGAGGT+TGG - chr8.1:45631153-45631172 None:intergenic 50.0%
TTGTTGAAGGTGGGGTCCAA+TGG - chr8.1:45631072-45631091 None:intergenic 50.0%
! ACCCCTTTGAAGGCGATCAA+AGG - chr8.1:45630997-45631016 None:intergenic 50.0%
AACCTGCAAAGTCTCCAGCC+TGG - chr8.1:45631420-45631439 None:intergenic 55.0%
AAGCTGTTCGAGCTGGAGGA+AGG - chr8.1:45631497-45631516 None:intergenic 55.0%
ACCTGCAAAGTCTCCAGCCT+GGG - chr8.1:45631419-45631438 None:intergenic 55.0%
GAATGTTGCGGGATTTGCGG+AGG - chr8.1:45630953-45630972 None:intergenic 55.0%
GGCTGCCATGTTCTCAATGG+TGG - chr8.1:45632141-45632160 None:intergenic 55.0%
TCCGGCTGCCATGTTCTCAA+TGG - chr8.1:45632144-45632163 None:intergenic 55.0%
TGCTCAACCTGTCGACGACT+GGG - chr8.1:45630746-45630765 None:intergenic 55.0%
TGGTGTCCCTTGTTGCTCGT+AGG + chr8.1:45632107-45632126 MS.gene34707:intron 55.0%
TGTCCCTTGTTGCTCGTAGG+AGG + chr8.1:45632110-45632129 MS.gene34707:intron 55.0%
! CGACAGGTTGAGCAGTCTAC+CGG + chr8.1:45630752-45630771 MS.gene34707:CDS 55.0%
AATGCGACCCAGTCGTCGAC+AGG + chr8.1:45630736-45630755 MS.gene34707:CDS 60.0%
CCTGCAAAGTCTCCAGCCTG+GGG - chr8.1:45631418-45631437 None:intergenic 60.0%
CTGCTCAACCTGTCGACGAC+TGG - chr8.1:45630747-45630766 None:intergenic 60.0%
GCACCAACCTCGTTTCCCTC+AGG + chr8.1:45631147-45631166 MS.gene34707:CDS 60.0%
GCGTGGATCCTTGCTGCCAT+TGG + chr8.1:45631053-45631072 MS.gene34707:CDS 60.0%
! CGATGAGCCTTGTCTCTCGC+AGG + chr8.1:45630829-45630848 MS.gene34707:CDS 60.0%
CCCCAGGCTGGAGACTTTGC+AGG + chr8.1:45631415-45631434 MS.gene34707:CDS 65.0%
GGTGGGGTCCAATGGCAGCA+AGG - chr8.1:45631064-45631083 None:intergenic 65.0%
! TCTTTCTCGCTGGCTGCCCC+AGG + chr8.1:45631399-45631418 MS.gene34707:CDS 65.0%
! TCTCGCTGGCTGCCCCAGGC+TGG + chr8.1:45631403-45631422 MS.gene34707:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 45630690 45632416 45630690 ID=MS.gene34707
chr8.1 mRNA 45630690 45632416 45630690 ID=MS.gene34707.t1;Parent=MS.gene34707
chr8.1 exon 45630690 45631168 45630690 ID=MS.gene34707.t1.exon1;Parent=MS.gene34707.t1
chr8.1 CDS 45630690 45631168 45630690 ID=cds.MS.gene34707.t1;Parent=MS.gene34707.t1
chr8.1 exon 45631280 45631569 45631280 ID=MS.gene34707.t1.exon2;Parent=MS.gene34707.t1
chr8.1 CDS 45631280 45631569 45631280 ID=cds.MS.gene34707.t1;Parent=MS.gene34707.t1
chr8.1 exon 45631656 45631759 45631656 ID=MS.gene34707.t1.exon3;Parent=MS.gene34707.t1
chr8.1 CDS 45631656 45631759 45631656 ID=cds.MS.gene34707.t1;Parent=MS.gene34707.t1
chr8.1 exon 45631854 45632003 45631854 ID=MS.gene34707.t1.exon4;Parent=MS.gene34707.t1
chr8.1 CDS 45631854 45632003 45631854 ID=cds.MS.gene34707.t1;Parent=MS.gene34707.t1
chr8.1 exon 45632129 45632416 45632129 ID=MS.gene34707.t1.exon5;Parent=MS.gene34707.t1
chr8.1 CDS 45632129 45632416 45632129 ID=cds.MS.gene34707.t1;Parent=MS.gene34707.t1
Gene Sequence

>MS.gene34707

ATGGCGGAGGAGTCAAGGATGATGAAACGACTGAAAGCACAAGAAGAATGCGACCCAGTCGTCGACAGGTTGAGCAGTCTACCGGACTCGTTGTTATGCCATATCATGTCCTTCCTCCCCACAAAAATTTCCGTTACTACGATGAGCCTTGTCTCTCGCAGGTATCTTCATCTCTGGAAGGATCTTCAGGTCTTGTATTTCGACTTTCACCACACAATTCCGTTCCAAACAGTTTCATCATTAGTAAACTCTGTTCTCGTCCTCCGCAAATCCCGCAACATTCAAAACTTCCATCTCACTTTCGCCTTTGATCGCCTTCAAAGGGGTTGTGTTCTTGAAAAGTTACAATCCAAGAATGCTGAAGCGTGGATCCTTGCTGCCATTGGACCCCACCTTCAACAACTTTATCTCAATGTTGATGGTTGTATAGATATCCCTCGTTCACTCTTTATCAATTGCACCAACCTCGTTTCCCTCAGGTAACTTCTGTTTTTAAATTAATTTTTAATCCCATCTTAATTGCAGGATACTCATGTTTTAATGTCCAAACACACTTTCACTTTTGAATCATACTTTTTCTAATACTGCAGTCTTCACGGTTCAATCGAAACGATGCACTGCAATATTAACAGTGCAAATTCCGTTTATTTTCCATCACTGAAGATGTTGTCTCTCGGCAATGATATTGTGGATCAGAACGAATTAGTTTTCTTTCTCGCTGGCTGCCCCAGGCTGGAGACTTTGCAGGTTTACGTTTACCTTTCTTCCGATGATGACATGTCTTTGACCAAAGTTCTAGTGACACCTTCCTCCAGCTCGAACAGCTTGATATCCGCTGATGACAATTTCACCTGGAGTTACTTTAATATAAATGAAGGACGTTTTAAATTGGGCATCGTTGGTAACTTTCGTAATATGGTGGAAGCATTTCTTGATGTTTTTTCCCGGCTGAAAGTGAATTTGTAGACGCTATTCTCAACCTCATCCAAGATACCAGAAGTGAATTTCATAGAAGCCTCATAATTAACTATGAGGATGTTTATCTACAGTTGCATCATTCGATGTCAAAGGTGAAGTTTTACTTTTATGGTTTACATGTGTTTAAATGTGTACTTTGGTTCTAATATTGTGTCGTTGTCACTTGTTGTTATCGTGTATGTGCAGTGGCCAACTCATGCACCTGTTCTAAATTATCCTGAATTTCGCGATTTACATCATTTGAAGTTCATCCTTCCATGTTTCAACTCAAATCTTCTGGTCCATTGTCTTGAGCAATGCCACATGCTTCAAGTTCTCATAATTCAGAGCAACAAGGTTTATTGGATTTTCAAATTTAATTCAAACATTCTTTGGTCGGTCTATATATACATGCATCTATGTCTCCATTTGTACACAAATATATTTTTGAGTCACGGCTTGGTGTCCCTTGTTGCTCGTAGGAGGAACAACCACCATTGAGAACATGGCAGCCGGAGTCAACAAGAGTTTCTGAATGTCTCAAGTCCCACCTGACTTATATTCACATAGAAGGATATCAAGGACTTGAAGATGAGCTGACATTTGCTGAATATATTTTGCGGAACGGACTTGTTTTGGAGACAATGCTTGTTTTTGTTGATATTTCAATGGACATAACAAACAAGTACCGTTCTATCAAAAGATTAACAAATGTAACGAAGGGCTCTGTCAAATGCCAACTTAAATTTGACCCAGATGTATCTTCTTAA
Protein sequence

>MS.gene34707.t1

MAEESRMMKRLKAQEECDPVVDRLSSLPDSLLCHIMSFLPTKISVTTMSLVSRRYLHLWKDLQVLYFDFHHTIPFQTVSSLVNSVLVLRKSRNIQNFHLTFAFDRLQRGCVLEKLQSKNAEAWILAAIGPHLQQLYLNVDGCIDIPRSLFINCTNLVSLSLHGSIETMHCNINSANSVYFPSLKMLSLGNDIVDQNELVFFLAGCPRLETLQVYVYLSSDDDMSLTKVLVTPSSSSNSLISADDNFTWSYFNINEGHAILNLIQDTRSEFHRSLIINYEDVYLQLHHSMSKWPTHAPVLNYPEFRDLHHLKFILPCFNSNLLVHCLEQCHMLQVLIIQSNKEEQPPLRTWQPESTRVSECLKSHLTYIHIEGYQGLEDELTFAEYILRNGLVLETMLVFVDISMDITNKYRSIKRLTNVTKGSVKCQLKFDPDVSS