AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64988

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64988.t1 MTR_8g062010 59.174 436 125 7 17 401 1 434 2.37e-155 445
MS.gene64988.t1 MTR_8g070950 56.319 451 126 9 6 401 4 438 1.51e-152 438
MS.gene64988.t1 MTR_8g062310 55.909 440 139 8 1 397 1 428 3.53e-151 435
MS.gene64988.t1 MTR_8g062080 54.860 463 135 11 1 402 1 450 2.07e-148 428
MS.gene64988.t1 MTR_8g058965 50.862 464 147 7 15 402 3 461 3.38e-138 403
MS.gene64988.t1 MTR_8g469020 49.451 455 148 6 24 401 6 455 1.60e-137 401
MS.gene64988.t1 MTR_8g062300 62.548 259 75 4 166 402 224 482 4.77e-100 306
MS.gene64988.t1 MTR_8g062300 45.238 168 76 5 5 164 7 166 4.74e-34 132
MS.gene64988.t1 MTR_2g086020 50.559 358 131 9 51 401 1 319 4.40e-95 288
MS.gene64988.t1 MTR_8g467790 42.857 378 125 9 25 342 148 494 5.83e-83 263
MS.gene64988.t1 MTR_2g083840 38.043 460 210 11 5 397 6 457 5.19e-76 243
MS.gene64988.t1 MTR_8g468810 38.085 470 207 17 4 397 2 463 2.26e-75 241
MS.gene64988.t1 MTR_8g062270 37.136 447 203 14 22 396 13 453 4.48e-72 233
MS.gene64988.t1 MTR_8g467800 36.422 464 206 14 9 397 4 453 4.78e-69 225
MS.gene64988.t1 MTR_8g058970 36.307 482 209 16 6 397 4 477 3.21e-68 223
MS.gene64988.t1 MTR_4g032730 34.483 464 214 13 18 396 8 466 4.52e-66 218
MS.gene64988.t1 MTR_5g092960 34.934 458 221 10 13 396 7 461 3.84e-63 210
MS.gene64988.t1 MTR_8g468840 35.068 442 209 12 25 395 14 448 3.24e-60 202
MS.gene64988.t1 MTR_4g050250 58.081 198 63 5 166 343 223 420 4.45e-60 201
MS.gene64988.t1 MTR_4g050250 48.837 172 78 3 1 166 1 168 4.31e-41 150
MS.gene64988.t1 MTR_8g014260 33.047 466 220 16 6 397 4 451 1.53e-55 189
MS.gene64988.t1 MTR_8g468760 35.477 451 183 15 19 396 3 418 2.16e-54 186
MS.gene64988.t1 MTR_8g077460 35.678 398 209 17 20 397 5 375 1.37e-50 174
MS.gene64988.t1 MTR_8g468810 36.000 375 164 13 4 307 2 371 5.14e-49 170
MS.gene64988.t1 MTR_8g445780 33.915 401 187 11 15 350 3 390 3.30e-44 158
MS.gene64988.t1 MTR_8g468810 36.913 298 121 9 4 238 2 295 1.93e-41 149
MS.gene64988.t1 MTR_8g014160 31.121 437 185 15 25 397 41 425 2.13e-41 151
MS.gene64988.t1 MTR_8g468810 36.913 298 121 9 4 238 2 295 3.02e-41 149
MS.gene64988.t1 MTR_8g468810 36.913 298 121 9 4 238 2 295 7.60e-41 148
MS.gene64988.t1 MTR_5g092960 33.607 366 173 8 12 307 6 371 9.87e-38 141
MS.gene64988.t1 MTR_8g014210 44.920 187 95 6 214 397 50 231 1.75e-34 128
MS.gene64988.t1 MTR_4g024710 42.424 198 108 6 202 397 154 347 1.65e-33 128
MS.gene64988.t1 MTR_8g467800 33.968 315 135 12 8 257 3 309 1.45e-30 120
MS.gene64988.t1 MTR_3g099800 63.218 87 31 1 309 394 19 105 2.23e-29 110
MS.gene64988.t1 MTR_5g008950 27.820 399 226 15 27 397 1 365 3.36e-27 111
MS.gene64988.t1 MTR_5g021550 42.604 169 79 8 5 164 3 162 3.88e-26 103
MS.gene64988.t1 MTR_1g041245 26.290 407 247 12 19 397 5 386 4.18e-26 108
MS.gene64988.t1 MTR_7g032380 27.366 391 223 13 24 397 10 356 1.71e-24 103
MS.gene64988.t1 MTR_3g107100 26.202 416 246 15 20 397 6 398 7.48e-24 102
MS.gene64988.t1 MTR_7g115270 27.295 414 243 13 19 394 6 399 1.06e-23 102
MS.gene64988.t1 MTR_4g024745 35.600 250 145 9 138 376 36 280 3.97e-23 98.6
MS.gene64988.t1 MTR_7g098510 27.361 413 253 14 6 397 5 391 1.16e-22 99.0
MS.gene64988.t1 MTR_7g056433 25.871 402 251 10 22 397 9 389 1.05e-21 96.3
MS.gene64988.t1 MTR_4g024720 36.813 182 83 7 6 164 4 176 3.56e-21 91.3
MS.gene64988.t1 MTR_7g098570 26.699 412 256 15 2 393 4 389 3.97e-21 95.5
MS.gene64988.t1 MTR_7g098480 27.103 428 231 13 6 396 5 388 9.73e-21 94.4
MS.gene64988.t1 MTR_3g107120 26.700 397 255 11 21 394 5 388 2.13e-19 89.7
MS.gene64988.t1 MTR_7g032370 25.858 379 235 13 37 397 2 352 3.81e-19 88.2
MS.gene64988.t1 MTR_8g014290 38.816 152 79 7 25 164 10 159 4.30e-19 84.0
MS.gene64988.t1 MTR_7g056277 24.165 389 258 13 25 396 259 627 6.45e-19 89.4
MS.gene64988.t1 MTR_6g022500 25.879 398 248 14 25 397 101 476 8.48e-19 88.6
MS.gene64988.t1 MTR_7g056420 26.276 392 235 13 17 389 1 357 2.44e-18 87.8
MS.gene64988.t1 MTR_7g056430 26.531 392 234 13 17 389 1 357 3.05e-18 87.4
MS.gene64988.t1 MTR_2g007550 25.974 385 206 14 24 394 13 332 3.26e-18 85.5
MS.gene64988.t1 MTR_3g105060 24.937 397 249 11 19 397 8 373 6.68e-18 85.1
MS.gene64988.t1 MTR_7g026500 25.871 402 247 14 25 396 14 394 9.92e-18 84.7
MS.gene64988.t1 MTR_8g014230 46.429 84 43 2 307 390 17 98 1.23e-17 78.2
MS.gene64988.t1 MTR_3g107160 25.871 402 247 15 22 397 7 383 4.11e-17 82.8
MS.gene64988.t1 MTR_7g034115 29.049 389 230 17 24 389 10 375 6.08e-17 82.0
MS.gene64988.t1 MTR_3g108150 26.515 396 249 13 23 396 11 386 1.01e-16 81.6
MS.gene64988.t1 MTR_7g007080 27.820 399 233 17 25 394 4 376 1.03e-16 81.6
MS.gene64988.t1 MTR_0543s0010 27.481 393 235 14 25 396 86 449 1.05e-16 82.0
MS.gene64988.t1 MTR_1g085120 25.355 422 252 17 10 397 79 471 1.98e-16 81.3
MS.gene64988.t1 MTR_1g085120 25.355 422 252 17 10 397 154 546 3.11e-16 80.9
MS.gene64988.t1 MTR_5g058000 24.740 384 260 13 25 396 8 374 2.86e-14 73.9
MS.gene64988.t1 MTR_8g085430 25.143 350 190 13 24 364 10 296 3.18e-14 73.6
MS.gene64988.t1 MTR_5g084070 32.447 188 108 6 212 389 241 419 5.62e-14 73.6
MS.gene64988.t1 MTR_6g022360 25.250 400 246 13 20 396 38 407 1.17e-13 72.4
MS.gene64988.t1 MTR_7g056447 33.333 153 86 5 17 166 1 140 2.66e-13 70.5
MS.gene64988.t1 MTR_2g005990 24.038 416 243 15 24 394 5 392 4.07e-13 70.9
MS.gene64988.t1 MTR_3g115430 36.937 111 64 3 288 397 20 125 9.54e-12 62.4
MS.gene64988.t1 MTR_5g082930 41.558 77 38 2 27 103 17 86 9.70e-12 64.3
MS.gene64988.t1 MTR_3g463830 29.518 166 101 5 15 177 53 205 1.27e-11 65.9
MS.gene64988.t1 MTR_4g081030 36.095 169 76 9 13 174 8 151 1.27e-11 66.2
MS.gene64988.t1 MTR_0009s0080 23.990 396 255 12 1 372 2 375 1.28e-11 66.2
MS.gene64988.t1 MTR_5g012840 26.796 362 209 16 43 397 6 318 1.40e-11 65.5
MS.gene64988.t1 MTR_3g040700 40.964 83 36 3 24 106 42 111 1.99e-11 65.9
MS.gene64988.t1 MTR_7g033915 33.951 162 85 7 242 396 218 364 3.01e-11 64.7
MS.gene64988.t1 MTR_3g023630 45.070 71 32 2 21 91 4 67 3.63e-11 64.7
MS.gene64988.t1 MTR_3g036250 39.535 86 39 3 22 107 2 74 8.42e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64988.t1 AT5G56420 25.959 443 236 18 23 397 4 422 1.20e-19 90.5
MS.gene64988.t1 AT5G56420 25.959 443 236 18 23 397 4 422 1.20e-19 90.5
MS.gene64988.t1 AT5G56420 25.959 443 236 18 23 397 4 422 1.20e-19 90.5
MS.gene64988.t1 AT5G53840 26.316 437 238 16 25 394 18 437 3.63e-17 83.6
MS.gene64988.t1 AT4G10400 26.168 428 239 17 25 396 2 408 1.52e-15 78.2
MS.gene64988.t1 AT4G10400 26.168 428 239 17 25 396 2 408 1.52e-15 78.2
MS.gene64988.t1 AT4G10400 26.168 428 239 17 25 396 2 408 1.52e-15 78.2
MS.gene64988.t1 AT1G78760 22.747 466 255 17 16 398 2 445 4.72e-14 73.9
MS.gene64988.t1 AT1G66310 34.459 148 82 8 22 166 16 151 5.62e-11 64.3

Find 97 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAGTCTTCAATGTTTCT+AGG 0.218658 8.4:-39592227 None:intergenic
TGGTTTAATATGTGAAATTC+TGG 0.234330 8.4:-39592627 None:intergenic
TCAAAACATTCAAAAGTTTC+AGG 0.251513 8.4:+39591612 MS.gene64988:CDS
TTTGAGGAACGGACTTGTTT+TGG 0.284546 8.4:+39593017 MS.gene64988:CDS
CTCGCCGGTGGCGGCATCTC+TGG 0.288539 8.4:+39591534 MS.gene64988:CDS
TGATCTACATTACTCCAGTT+TGG 0.296579 8.4:+39592338 MS.gene64988:CDS
TGTGGCTCCCATGTTCTTAA+AGG 0.320804 8.4:-39592895 None:intergenic
ATGTGGATCCGTGCTGCTAC+TGG 0.325587 8.4:+39591752 MS.gene64988:CDS
GACCCAACTGTATCTTCTTA+AGG 0.326344 8.4:+39593150 MS.gene64988:CDS
CTCTTGGAGCTGGAGGAAAT+TGG 0.330868 8.4:-39592276 None:intergenic
AAGAGCAGAGGGAGGTCTAT+TGG 0.334713 8.4:-39591812 None:intergenic
GATCTACATTACTCCAGTTT+GGG 0.352426 8.4:+39592339 MS.gene64988:CDS
GACAGAGCCCCTTGGTATAT+CGG 0.361316 8.4:-39593109 None:intergenic
TACTCCAGTTTGGGCATTGT+TGG 0.373038 8.4:+39592348 MS.gene64988:CDS
CGTCGTTTAGAGCTTAATTT+CGG 0.374090 8.4:-39591377 None:intergenic
ATTGGTGGATTTCAAGCTCT+TGG 0.379804 8.4:-39592292 None:intergenic
TTCGAAGATTGCGTTTCTGC+TGG 0.381651 8.4:+39591687 MS.gene64988:CDS
AAGAAGATTTGAGTTGAAGC+AGG 0.383028 8.4:-39592667 None:intergenic
AGGGGAGATAGAACTTTAGA+TGG 0.392720 8.4:-39592647 None:intergenic
CACAACGGTCTTTGAGAATA+GGG 0.394548 8.4:-39592437 None:intergenic
TTCGAATATCGCGGGATTTG+CGG 0.397797 8.4:-39591670 None:intergenic
TTCCAACTAGAACCTCTGTT+TGG 0.407347 8.4:+39591495 MS.gene64988:CDS
ACACAACGGTCTTTGAGAAT+AGG 0.411344 8.4:-39592438 None:intergenic
GGATTTCAAGCTCTTGGAGC+TGG 0.411420 8.4:-39592286 None:intergenic
GCATCTCTGGAAGGATATCC+AGG 0.415139 8.4:+39591547 MS.gene64988:CDS
ATATCCTTCCATGTGAATAT+AGG 0.416098 8.4:-39592956 None:intergenic
GTTGCCAACAATGCCCAAAC+TGG 0.420991 8.4:-39592352 None:intergenic
TGCTGGGATTTCAGTGATAA+CGG 0.437009 8.4:+39591704 MS.gene64988:CDS
TCCCATGTTCTTAAAGGTGA+TGG 0.457043 8.4:-39592889 None:intergenic
GCCTTGTTTCTCGCCGGTGG+CGG 0.469770 8.4:+39591525 MS.gene64988:CDS
AGAAGATTTGAGTTGAAGCA+GGG 0.486160 8.4:-39592666 None:intergenic
GACATGATATGACACAGGAT+TGG 0.488583 8.4:-39591467 None:intergenic
AGGTGGGATTTGAGACATCT+AGG 0.491228 8.4:-39592931 None:intergenic
GCACAAATCTCGTTTCCCTC+TGG 0.491611 8.4:+39591843 MS.gene64988:CDS
ACTCAGCCTGTCGGCGTGGT+CGG 0.501150 8.4:-39591432 None:intergenic
ACATGATATGACACAGGATT+GGG 0.502293 8.4:-39591466 None:intergenic
TGGCAACTTTCACAGTATGA+TGG 0.508985 8.4:+39592368 MS.gene64988:CDS
GGAAAGAAATAAAAGAAGTC+GGG 0.509568 8.4:+39591402 MS.gene64988:CDS
ATTCACATGGAAGGATATCA+AGG 0.509844 8.4:+39592961 MS.gene64988:CDS
CTATGAGCCTTGTTTCTCGC+CGG 0.510412 8.4:+39591519 MS.gene64988:CDS
TGTGGATCCGTGCTGCTACT+GGG 0.516513 8.4:+39591753 MS.gene64988:CDS
CTTCCATGTGAATATAGGTC+AGG 0.517916 8.4:-39592951 None:intergenic
CACCTTAAGAAGATACAGTT+GGG 0.520063 8.4:-39593152 None:intergenic
GTCCAAACAGAGGTTCTAGT+TGG 0.524498 8.4:-39591497 None:intergenic
GAGACACCGACCACGCCGAC+AGG 0.524750 8.4:+39591426 MS.gene64988:CDS
TCGAAGATTGCGTTTCTGCT+GGG 0.525303 8.4:+39591688 MS.gene64988:CDS
CCACCAATGACAGTTTCACT+TGG 0.531873 8.4:+39592307 MS.gene64988:CDS
GAAGATTTGAGTTGAAGCAG+GGG 0.532470 8.4:-39592665 None:intergenic
CCAAGTGAAACTGTCATTGG+TGG 0.535449 8.4:-39592307 None:intergenic
CCTTCCAGAGATGCCGCCAC+CGG 0.535690 8.4:-39591538 None:intergenic
ATATGACACAGGATTGGGTC+TGG 0.535975 8.4:-39591461 None:intergenic
GAGCTGGAGGAAATTGGAAT+GGG 0.542677 8.4:-39592270 None:intergenic
GGAGCTGGAGGAAATTGGAA+TGG 0.543028 8.4:-39592271 None:intergenic
CAGTTGGGTCAAATTTAAGT+TGG 0.543112 8.4:-39593137 None:intergenic
ACTCCAAGTGAAACTGTCAT+TGG 0.547172 8.4:-39592310 None:intergenic
TTTGAATCATTCGACATCAA+AGG 0.551465 8.4:+39592482 MS.gene64988:CDS
TGAGCCTTGTTTCTCGCCGG+TGG 0.553094 8.4:+39591522 MS.gene64988:CDS
AACCATCACCTTTAAGAACA+TGG 0.553947 8.4:+39592887 MS.gene64988:CDS
TTTCAAGCTCTTGGAGCTGG+AGG 0.556210 8.4:-39592283 None:intergenic
CGGAAAGAAATAAAAGAAGT+CGG 0.556587 8.4:+39591401 MS.gene64988:CDS
AAACAGAGGTTCTAGTTGGA+AGG 0.556788 8.4:-39591493 None:intergenic
TGCAATTGATCAAGAGCAGA+GGG 0.558582 8.4:-39591823 None:intergenic
TCACCTTAAGAAGATACAGT+TGG 0.569009 8.4:-39593153 None:intergenic
CCATGTGAATATAGGTCAGG+TGG 0.571828 8.4:-39592948 None:intergenic
GCCGCCACCGGCGAGAAACA+AGG 0.581049 8.4:-39591526 None:intergenic
ACGCAATCTTCGAATATCGC+GGG 0.585451 8.4:-39591678 None:intergenic
CCGGTGGCGGCATCTCTGGA+AGG 0.587366 8.4:+39591538 MS.gene64988:CDS
AAACATTCAAAAGTTTCAGG+AGG 0.590073 8.4:+39591615 MS.gene64988:CDS
GACTACTCAGCCTGTCGGCG+TGG 0.593009 8.4:-39591436 None:intergenic
ACCATCACCTTTAAGAACAT+GGG 0.597069 8.4:+39592888 MS.gene64988:CDS
AACCTTCGAAGTTAAAACCC+TGG 0.599098 8.4:-39591565 None:intergenic
TGGCAGTTGACAGAGCCCCT+TGG 0.599352 8.4:-39593117 None:intergenic
AACTTCGAAGGTTACGAGGT+AGG 0.599788 8.4:+39591575 MS.gene64988:CDS
TTCTGGATAATTTAGAACAG+TGG 0.600297 8.4:-39592610 None:intergenic
AAAGCGAATGTGCTGAAATG+TGG 0.606585 8.4:+39591735 MS.gene64988:CDS
AAGATTAACCGATATACCAA+GGG 0.609206 8.4:+39593101 MS.gene64988:CDS
AGGTTACGAGGTAGGTCAGA+GGG 0.611309 8.4:+39591583 MS.gene64988:CDS
CCACCTGACCTATATTCACA+TGG 0.613292 8.4:+39592948 MS.gene64988:CDS
TTTAGAACAGTGGCATGAAG+TGG 0.614365 8.4:-39592600 None:intergenic
AAAGATTAACCGATATACCA+AGG 0.616862 8.4:+39593100 MS.gene64988:CDS
GAATATCGCGGGATTTGCGG+AGG 0.617040 8.4:-39591667 None:intergenic
CTGACCTATATTCACATGGA+AGG 0.622931 8.4:+39592952 MS.gene64988:CDS
CATGTGAATATAGGTCAGGT+GGG 0.630464 8.4:-39592947 None:intergenic
GGTGGGGCCCAGTAGCAGCA+CGG 0.641352 8.4:-39591760 None:intergenic
AACGCAATCTTCGAATATCG+CGG 0.643002 8.4:-39591679 None:intergenic
GTGCAATTGATCAAGAGCAG+AGG 0.648148 8.4:-39591824 None:intergenic
GGAAAGACATGATATGACAC+AGG 0.659124 8.4:-39591472 None:intergenic
CATCTCTGGAAGGATATCCA+GGG 0.666418 8.4:+39591548 MS.gene64988:CDS
TCTCATAATTAAGAGCAACA+AGG 0.667425 8.4:+39592725 MS.gene64988:CDS
AAGGTTACGAGGTAGGTCAG+AGG 0.667975 8.4:+39591582 MS.gene64988:CDS
CTAGGAACTGTTGTTGACTG+TGG 0.669916 8.4:-39592913 None:intergenic
TGGTAGACTACTCAGCCTGT+CGG 0.688064 8.4:-39591441 None:intergenic
GTAAATAATCATCAACACAA+CGG 0.705260 8.4:-39592452 None:intergenic
AATTAAGCTCTAAACGACGA+CGG 0.722164 8.4:+39591381 MS.gene64988:CDS
AATTGATCAAGAGCAGAGGG+AGG 0.726934 8.4:-39591820 None:intergenic
AGATTAACCGATATACCAAG+GGG 0.738387 8.4:+39593102 MS.gene64988:CDS
AAGGCTCATAGTCCAAACAG+AGG 0.744795 8.4:-39591507 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATCATTATATTTAAAATTA+AGG + chr8.4:39591915-39591934 MS.gene64988:intron 10.0%
!! AAAATCTAAAAATAGCAAGT+AGG - chr8.4:39591883-39591902 None:intergenic 20.0%
!! ACTTTAATTCAATCAATCTT+TGG + chr8.4:39592764-39592783 MS.gene64988:intron 20.0%
!! TTGTATATAATAACAAGTGA+TGG - chr8.4:39592573-39592592 None:intergenic 20.0%
!!! TATTTTTGTTGATACTTCAA+TGG + chr8.4:39593050-39593069 MS.gene64988:CDS 20.0%
! GTAAATAATCATCAACACAA+CGG - chr8.4:39592455-39592474 None:intergenic 25.0%
! TCAAAACATTCAAAAGTTTC+AGG + chr8.4:39591612-39591631 MS.gene64988:CDS 25.0%
! TGGTTTAATATGTGAAATTC+TGG - chr8.4:39592630-39592649 None:intergenic 25.0%
!! AGGTGAAGTTTAAACTTTTA+TGG + chr8.4:39592502-39592521 MS.gene64988:intron 25.0%
!! TTCATTTTACTATACACAAG+AGG + chr8.4:39592245-39592264 MS.gene64988:CDS 25.0%
!! TTGTTATTATATACAAGCAG+CGG + chr8.4:39592577-39592596 MS.gene64988:intron 25.0%
!!! CATTTGCTGAATATATTTTG+AGG + chr8.4:39593001-39593020 MS.gene64988:CDS 25.0%
!!! TTGACAAAATGTTTTAACTG+TGG + chr8.4:39592010-39592029 MS.gene64988:intron 25.0%
AAACATTCAAAAGTTTCAGG+AGG + chr8.4:39591615-39591634 MS.gene64988:CDS 30.0%
AAAGATTAACCGATATACCA+AGG + chr8.4:39593100-39593119 MS.gene64988:CDS 30.0%
AAGATTAACCGATATACCAA+GGG + chr8.4:39593101-39593120 MS.gene64988:CDS 30.0%
ACAAGTATACATTTGAGTCA+TGG + chr8.4:39592835-39592854 MS.gene64988:intron 30.0%
ATATCCTTCCATGTGAATAT+AGG - chr8.4:39592959-39592978 None:intergenic 30.0%
ATGAAGTCTTCAATGTTTCT+AGG - chr8.4:39592230-39592249 None:intergenic 30.0%
CGGAAAGAAATAAAAGAAGT+CGG + chr8.4:39591401-39591420 MS.gene64988:CDS 30.0%
GGAAAGAAATAAAAGAAGTC+GGG + chr8.4:39591402-39591421 MS.gene64988:CDS 30.0%
TCTCATAATTAAGAGCAACA+AGG + chr8.4:39592725-39592744 MS.gene64988:CDS 30.0%
TTTGAATCATTCGACATCAA+AGG + chr8.4:39592482-39592501 MS.gene64988:CDS 30.0%
! ATAGCAAGTAGGTTGAAATT+AGG - chr8.4:39591872-39591891 None:intergenic 30.0%
! ATTTCTTGATGTCTTTTCTC+TGG + chr8.4:39592395-39592414 MS.gene64988:CDS 30.0%
! TTCTGGATAATTTAGAACAG+TGG - chr8.4:39592613-39592632 None:intergenic 30.0%
!! GCTGAATATATTTTGAGGAA+CGG + chr8.4:39593006-39593025 MS.gene64988:CDS 30.0%
AAAAACAGTTCTTGAAGGTG+GGG - chr8.4:39591779-39591798 None:intergenic 35.0%
AAGAACCTGGAATTCGAAAA+TGG + chr8.4:39592162-39592181 MS.gene64988:intron 35.0%
ACATGATATGACACAGGATT+GGG - chr8.4:39591469-39591488 None:intergenic 35.0%
ACCAGAAAAACAGTTCTTGA+AGG - chr8.4:39591784-39591803 None:intergenic 35.0%
AGAAAAACAGTTCTTGAAGG+TGG - chr8.4:39591781-39591800 None:intergenic 35.0%
AGATTAACCGATATACCAAG+GGG + chr8.4:39593102-39593121 MS.gene64988:CDS 35.0%
AGGTGGTTTAGTCGAAATAA+AGG + chr8.4:39592101-39592120 MS.gene64988:intron 35.0%
ATTCACATGGAAGGATATCA+AGG + chr8.4:39592961-39592980 MS.gene64988:CDS 35.0%
CAGTTGGGTCAAATTTAAGT+TGG - chr8.4:39593140-39593159 None:intergenic 35.0%
GAAAAACAGTTCTTGAAGGT+GGG - chr8.4:39591780-39591799 None:intergenic 35.0%
GATCTACATTACTCCAGTTT+GGG + chr8.4:39592339-39592358 MS.gene64988:CDS 35.0%
GTTGAAATTAGGTTACCAGA+GGG - chr8.4:39591861-39591880 None:intergenic 35.0%
TGATCTACATTACTCCAGTT+TGG + chr8.4:39592338-39592357 MS.gene64988:CDS 35.0%
! AACCATCACCTTTAAGAACA+TGG + chr8.4:39592887-39592906 MS.gene64988:CDS 35.0%
! ACCATCACCTTTAAGAACAT+GGG + chr8.4:39592888-39592907 MS.gene64988:CDS 35.0%
! CGTCGTTTAGAGCTTAATTT+CGG - chr8.4:39591380-39591399 None:intergenic 35.0%
!! AAGAAGATTTGAGTTGAAGC+AGG - chr8.4:39592670-39592689 None:intergenic 35.0%
!! AATTAAGCTCTAAACGACGA+CGG + chr8.4:39591381-39591400 MS.gene64988:CDS 35.0%
!! ACCTTCAAGAACTGTTTTTC+TGG + chr8.4:39591780-39591799 MS.gene64988:CDS 35.0%
!! AGAAGATTTGAGTTGAAGCA+GGG - chr8.4:39592669-39592688 None:intergenic 35.0%
!!! TTTTAACTTCGAAGGTTACG+AGG + chr8.4:39591571-39591590 MS.gene64988:CDS 35.0%
AAAGCGAATGTGCTGAAATG+TGG + chr8.4:39591735-39591754 MS.gene64988:CDS 40.0%
AACACTGCAGTCTTCTATCT+AGG + chr8.4:39592081-39592100 MS.gene64988:intron 40.0%
AACCTTCGAAGTTAAAACCC+TGG - chr8.4:39591568-39591587 None:intergenic 40.0%
AACGCAATCTTCGAATATCG+CGG - chr8.4:39591682-39591701 None:intergenic 40.0%
AATTCCAGGTTCTTGAGTGA+TGG - chr8.4:39592156-39592175 None:intergenic 40.0%
ACTCCAAGTGAAACTGTCAT+TGG - chr8.4:39592313-39592332 None:intergenic 40.0%
AGGGGAGATAGAACTTTAGA+TGG - chr8.4:39592650-39592669 None:intergenic 40.0%
CATGTGAATATAGGTCAGGT+GGG - chr8.4:39592950-39592969 None:intergenic 40.0%
CTGACCTATATTCACATGGA+AGG + chr8.4:39592952-39592971 MS.gene64988:CDS 40.0%
CTTCCATGTGAATATAGGTC+AGG - chr8.4:39592954-39592973 None:intergenic 40.0%
GACATGATATGACACAGGAT+TGG - chr8.4:39591470-39591489 None:intergenic 40.0%
GGAAAGACATGATATGACAC+AGG - chr8.4:39591475-39591494 None:intergenic 40.0%
GGTTGAAATTAGGTTACCAG+AGG - chr8.4:39591862-39591881 None:intergenic 40.0%
TCCCATGTTCTTAAAGGTGA+TGG - chr8.4:39592892-39592911 None:intergenic 40.0%
TGCAATTGATCAAGAGCAGA+GGG - chr8.4:39591826-39591845 None:intergenic 40.0%
TGCTGGGATTTCAGTGATAA+CGG + chr8.4:39591704-39591723 MS.gene64988:CDS 40.0%
TGGCAACTTTCACAGTATGA+TGG + chr8.4:39592368-39592387 MS.gene64988:CDS 40.0%
TTCCAACTAGAACCTCTGTT+TGG + chr8.4:39591495-39591514 MS.gene64988:CDS 40.0%
! AAACAGAGGTTCTAGTTGGA+AGG - chr8.4:39591496-39591515 None:intergenic 40.0%
! ACACAACGGTCTTTGAGAAT+AGG - chr8.4:39592441-39592460 None:intergenic 40.0%
! CACAACGGTCTTTGAGAATA+GGG - chr8.4:39592440-39592459 None:intergenic 40.0%
! TTTAGAACAGTGGCATGAAG+TGG - chr8.4:39592603-39592622 None:intergenic 40.0%
! TTTTCCATCACTCAAGAACC+TGG + chr8.4:39592149-39592168 MS.gene64988:intron 40.0%
!! AGTCTTCAATGTTTCTAGGC+TGG - chr8.4:39592226-39592245 None:intergenic 40.0%
!! ATTGGTGGATTTCAAGCTCT+TGG - chr8.4:39592295-39592314 None:intergenic 40.0%
!! GAAGATTTGAGTTGAAGCAG+GGG - chr8.4:39592668-39592687 None:intergenic 40.0%
!! GTCTTCAATGTTTCTAGGCT+GGG - chr8.4:39592225-39592244 None:intergenic 40.0%
!! TCTTCAATGTTTCTAGGCTG+GGG - chr8.4:39592224-39592243 None:intergenic 40.0%
!! TTTGAGGAACGGACTTGTTT+TGG + chr8.4:39593017-39593036 MS.gene64988:CDS 40.0%
!!! ATCCAGGGTTTTAACTTCGA+AGG + chr8.4:39591563-39591582 MS.gene64988:CDS 40.0%
AAAGGCAGCTACTGAATCGA+CGG - chr8.4:39592190-39592209 None:intergenic 45.0%
AACTTCGAAGGTTACGAGGT+AGG + chr8.4:39591575-39591594 MS.gene64988:CDS 45.0%
AAGGCTCATAGTCCAAACAG+AGG - chr8.4:39591510-39591529 None:intergenic 45.0%
AATTGATCAAGAGCAGAGGG+AGG - chr8.4:39591823-39591842 None:intergenic 45.0%
ACGCAATCTTCGAATATCGC+GGG - chr8.4:39591681-39591700 None:intergenic 45.0%
ACTGCAGTCTTCTATCTAGG+TGG + chr8.4:39592084-39592103 MS.gene64988:intron 45.0%
ATATGACACAGGATTGGGTC+TGG - chr8.4:39591464-39591483 None:intergenic 45.0%
CCAAGTGAAACTGTCATTGG+TGG - chr8.4:39592310-39592329 None:intergenic 45.0%
CCACCAATGACAGTTTCACT+TGG + chr8.4:39592307-39592326 MS.gene64988:CDS 45.0%
CCACCTGACCTATATTCACA+TGG + chr8.4:39592948-39592967 MS.gene64988:CDS 45.0%
CCATGTGAATATAGGTCAGG+TGG - chr8.4:39592951-39592970 None:intergenic 45.0%
GAAGACTGCAGTGTTTGCAA+AGG - chr8.4:39592076-39592095 None:intergenic 45.0%
GAGCTGGAGGAAATTGGAAT+GGG - chr8.4:39592273-39592292 None:intergenic 45.0%
GTGCAATTGATCAAGAGCAG+AGG - chr8.4:39591827-39591846 None:intergenic 45.0%
TCGAAGATTGCGTTTCTGCT+GGG + chr8.4:39591688-39591707 MS.gene64988:CDS 45.0%
TGTGGCTCCCATGTTCTTAA+AGG - chr8.4:39592898-39592917 None:intergenic 45.0%
TTCGAAGATTGCGTTTCTGC+TGG + chr8.4:39591687-39591706 MS.gene64988:CDS 45.0%
TTCGAATATCGCGGGATTTG+CGG - chr8.4:39591673-39591692 None:intergenic 45.0%
! AGGTGGGATTTGAGACATCT+AGG - chr8.4:39592934-39592953 None:intergenic 45.0%
! CATCTCTGGAAGGATATCCA+GGG + chr8.4:39591548-39591567 MS.gene64988:CDS 45.0%
! CTAGGAACTGTTGTTGACTG+TGG - chr8.4:39592916-39592935 None:intergenic 45.0%
! GTCCAAACAGAGGTTCTAGT+TGG - chr8.4:39591500-39591519 None:intergenic 45.0%
! TACTCCAGTTTGGGCATTGT+TGG + chr8.4:39592348-39592367 MS.gene64988:CDS 45.0%
AAGAGCAGAGGGAGGTCTAT+TGG - chr8.4:39591815-39591834 None:intergenic 50.0%
AAGGTTACGAGGTAGGTCAG+AGG + chr8.4:39591582-39591601 MS.gene64988:CDS 50.0%
AGGTTACGAGGTAGGTCAGA+GGG + chr8.4:39591583-39591602 MS.gene64988:CDS 50.0%
CCTACCAGCAACAAAGGACA+GGG - chr8.4:39592864-39592883 None:intergenic 50.0%
CTCTTGGAGCTGGAGGAAAT+TGG - chr8.4:39592279-39592298 None:intergenic 50.0%
GACAGAGCCCCTTGGTATAT+CGG - chr8.4:39593112-39593131 None:intergenic 50.0%
GCACAAATCTCGTTTCCCTC+TGG + chr8.4:39591843-39591862 MS.gene64988:CDS 50.0%
GGAGCTGGAGGAAATTGGAA+TGG - chr8.4:39592274-39592293 None:intergenic 50.0%
GGATTTCAAGCTCTTGGAGC+TGG - chr8.4:39592289-39592308 None:intergenic 50.0%
GTTCCTCCTACCAGCAACAA+AGG - chr8.4:39592870-39592889 None:intergenic 50.0%
GTTGCCAACAATGCCCAAAC+TGG - chr8.4:39592355-39592374 None:intergenic 50.0%
TCCTACCAGCAACAAAGGAC+AGG - chr8.4:39592865-39592884 None:intergenic 50.0%
TGGTAGACTACTCAGCCTGT+CGG - chr8.4:39591444-39591463 None:intergenic 50.0%
TGTCCTTTGTTGCTGGTAGG+AGG + chr8.4:39592864-39592883 MS.gene64988:intron 50.0%
TTTCAAGCTCTTGGAGCTGG+AGG - chr8.4:39592286-39592305 None:intergenic 50.0%
! GCATCTCTGGAAGGATATCC+AGG + chr8.4:39591547-39591566 MS.gene64988:CDS 50.0%
!! CTATGAGCCTTGTTTCTCGC+CGG + chr8.4:39591519-39591538 MS.gene64988:CDS 50.0%
!!! CGGTGCCATTTTCGAATTCC+AGG - chr8.4:39592170-39592189 None:intergenic 50.0%
CCCTGTCCTTTGTTGCTGGT+AGG + chr8.4:39592861-39592880 MS.gene64988:intron 55.0%
GAATATCGCGGGATTTGCGG+AGG - chr8.4:39591670-39591689 None:intergenic 55.0%
GCTGGGGCAGTTAGAGAGAA+AGG - chr8.4:39592208-39592227 None:intergenic 55.0%
GTGTCCCTGTCCTTTGTTGC+TGG + chr8.4:39592857-39592876 MS.gene64988:intron 55.0%
! ATGTGGATCCGTGCTGCTAC+TGG + chr8.4:39591752-39591771 MS.gene64988:CDS 55.0%
! TGTGGATCCGTGCTGCTACT+GGG + chr8.4:39591753-39591772 MS.gene64988:CDS 55.0%
TGGCAGTTGACAGAGCCCCT+TGG - chr8.4:39593120-39593139 None:intergenic 60.0%
!! TGAGCCTTGTTTCTCGCCGG+TGG + chr8.4:39591522-39591541 MS.gene64988:CDS 60.0%
ACTCAGCCTGTCGGCGTGGT+CGG - chr8.4:39591435-39591454 None:intergenic 65.0%
CCTTCCAGAGATGCCGCCAC+CGG - chr8.4:39591541-39591560 None:intergenic 65.0%
GACTACTCAGCCTGTCGGCG+TGG - chr8.4:39591439-39591458 None:intergenic 65.0%
!! GCCTTGTTTCTCGCCGGTGG+CGG + chr8.4:39591525-39591544 MS.gene64988:CDS 65.0%
GAGACACCGACCACGCCGAC+AGG + chr8.4:39591426-39591445 MS.gene64988:CDS 70.0%
GCCGCCACCGGCGAGAAACA+AGG - chr8.4:39591529-39591548 None:intergenic 70.0%
GGTGGGGCCCAGTAGCAGCA+CGG - chr8.4:39591763-39591782 None:intergenic 70.0%
! CCGGTGGCGGCATCTCTGGA+AGG + chr8.4:39591538-39591557 MS.gene64988:CDS 70.0%
! CTCGCCGGTGGCGGCATCTC+TGG + chr8.4:39591534-39591553 MS.gene64988:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 39591371 39593170 39591371 ID=MS.gene64988
chr8.4 mRNA 39591371 39593170 39591371 ID=MS.gene64988.t1;Parent=MS.gene64988
chr8.4 exon 39591371 39591864 39591371 ID=MS.gene64988.t1.exon1;Parent=MS.gene64988.t1
chr8.4 CDS 39591371 39591864 39591371 ID=cds.MS.gene64988.t1;Parent=MS.gene64988.t1
chr8.4 exon 39592227 39592503 39592227 ID=MS.gene64988.t1.exon2;Parent=MS.gene64988.t1
chr8.4 CDS 39592227 39592503 39592227 ID=cds.MS.gene64988.t1;Parent=MS.gene64988.t1
chr8.4 exon 39592597 39592746 39592597 ID=MS.gene64988.t1.exon3;Parent=MS.gene64988.t1
chr8.4 CDS 39592597 39592746 39592597 ID=cds.MS.gene64988.t1;Parent=MS.gene64988.t1
chr8.4 exon 39592883 39593170 39592883 ID=MS.gene64988.t1.exon4;Parent=MS.gene64988.t1
chr8.4 CDS 39592883 39593170 39592883 ID=cds.MS.gene64988.t1;Parent=MS.gene64988.t1
Gene Sequence

>MS.gene64988

ATGGCGCCGAAATTAAGCTCTAAACGACGACGGAAAGAAATAAAAGAAGTCGGGAGAGACACCGACCACGCCGACAGGCTGAGTAGTCTACCAGACCCAATCCTGTGTCATATCATGTCTTTCCTTCCAACTAGAACCTCTGTTTGGACTATGAGCCTTGTTTCTCGCCGGTGGCGGCATCTCTGGAAGGATATCCAGGGTTTTAACTTCGAAGGTTACGAGGTAGGTCAGAGGGAAAGCTTCAAAACATTCAAAAGTTTCAGGAGGTTTGCATCATTCGTGAACAATGTGCTCATCCTCCGCAAATCCCGCGATATTCGAAGATTGCGTTTCTGCTGGGATTTCAGTGATAACGGCGACTTTCAAAGCGAATGTGCTGAAATGTGGATCCGTGCTGCTACTGGGCCCCACCTTCAAGAACTGTTTTTCTGGTCAGACGCTCCAATAGACCTCCCTCTGCTCTTGATCAATTGCACAAATCTCGTTTCCCTCTGGTAACCTAATTTCAACCTACTTGCTATTTTTAGATTTTTTTTTCTAGTTTCATCATTATATTTAAAATTAAGGTAGAATGAGATGAAAACAAAAGCTTATAATTTAGCTGACTTCTGTTTTAAATTAATTTTTAATACTATGAAATTGACAAAATGTTTTAACTGTGGAAATGAAAAGTGTTAACAATACTCTTGTTTTAATGTCTATCCTTTGCAAACACTGCAGTCTTCTATCTAGGTGGTTTAGTCGAAATAAAGGTTCAACTTCAACATTCTTCACTTCATTTTCCATCACTCAAGAACCTGGAATTCGAAAATGGCACCGTCGATTCAGTAGCTGCCTTTCTCTCTAACTGCCCCAGCCTAGAAACATTGAAGACTTCATTTTACTATACACAAGAGGTTCCCATTCCAATTTCCTCCAGCTCCAAGAGCTTGAAATCCACCAATGACAGTTTCACTTGGAGTTACTTTGATCTACATTACTCCAGTTTGGGCATTGTTGGCAACTTTCACAGTATGATGGAAGCATTTCTTGATGTCTTTTCTCTGGCTGAAAGTGAATTTGTTGACCCTATTCTCAAAGACCGTTGTGTTGATGATTATTTACATCTAGATTTGAATCATTCGACATCAAAGGTGAAGTTTAAACTTTTATGGTTTATATATATGTACTTGTATATGTAGTTCTGTACTAACATTGTGCCATCACTTGTTATTATATACAAGCAGCGGCCACTTCATGCCACTGTTCTAAATTATCCAGAATTTCACATATTAAACCATCTAAAGTTCTATCTCCCCTGCTTCAACTCAAATCTTCTTCTCAACGTGCTTGATAAATGTCATATGCTTCAAGTTCTCATAATTAAGAGCAACAAGGTTTGTTTCACTTCTCAACTTTAATTCAATCAATCTTTGGTCTGTGTGTGCGTGTGTGTATGTGTGTTTGTATCTGTATAGTACACACACAAGTATACATTTGAGTCATGGTGTCCCTGTCCTTTGTTGCTGGTAGGAGGAACCATCACCTTTAAGAACATGGGAGCCACAGTCAACAACAGTTCCTAGATGTCTCAAATCCCACCTGACCTATATTCACATGGAAGGATATCAAGGATTTGAAGATGAGCTGACATTTGCTGAATATATTTTGAGGAACGGACTTGTTTTGGAGACGATGCTTATTTTTGTTGATACTTCAATGGACATAACAAATAAGTACTGTTCTGTCAAAAGATTAACCGATATACCAAGGGGCTCTGTCAACTGCCAACTTAAATTTGACCCAACTGTATCTTCTTAA
Protein sequence

>MS.gene64988.t1

MAPKLSSKRRRKEIKEVGRDTDHADRLSSLPDPILCHIMSFLPTRTSVWTMSLVSRRWRHLWKDIQGFNFEGYEVGQRESFKTFKSFRRFASFVNNVLILRKSRDIRRLRFCWDFSDNGDFQSECAEMWIRAATGPHLQELFFWSDAPIDLPLLLINCTNLVSLCLETLKTSFYYTQEVPIPISSSSKSLKSTNDSFTWSYFDLHYSSLGIVGNFHSMMEAFLDVFSLAESEFVDPILKDRCVDDYLHLDLNHSTSKRPLHATVLNYPEFHILNHLKFYLPCFNSNLLLNVLDKCHMLQVLIIKSNKEEPSPLRTWEPQSTTVPRCLKSHLTYIHMEGYQGFEDELTFAEYILRNGLVLETMLIFVDTSMDITNKYCSVKRLTDIPRGSVNCQLKFDPTVSS