AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25352

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25352.t1 MTR_8g062300 60.808 495 137 10 1 456 1 477 0.0 530
MS.gene25352.t1 MTR_8g070950 57.451 463 158 11 3 456 2 434 5.99e-158 454
MS.gene25352.t1 MTR_8g062010 58.186 452 160 12 12 456 1 430 6.59e-153 441
MS.gene25352.t1 MTR_8g062310 54.525 442 166 8 20 460 25 432 3.47e-149 432
MS.gene25352.t1 MTR_8g062080 53.696 460 177 11 8 456 11 445 1.92e-147 428
MS.gene25352.t1 MTR_8g469020 48.305 472 186 12 18 456 5 451 1.28e-134 395
MS.gene25352.t1 MTR_8g058965 49.061 479 188 14 11 456 1 456 5.29e-131 387
MS.gene25352.t1 MTR_4g050250 47.404 443 169 15 1 402 1 420 1.33e-109 331
MS.gene25352.t1 MTR_8g467790 45.592 397 151 11 20 401 148 494 1.18e-94 295
MS.gene25352.t1 MTR_8g468810 38.509 483 245 16 4 456 3 463 2.46e-74 241
MS.gene25352.t1 MTR_2g083840 37.553 474 249 13 6 456 8 457 1.32e-73 239
MS.gene25352.t1 MTR_8g062270 36.989 465 237 15 20 455 16 453 2.70e-72 235
MS.gene25352.t1 MTR_8g058970 37.475 491 253 17 4 456 3 477 4.23e-71 233
MS.gene25352.t1 MTR_2g086020 79.433 141 29 0 316 456 175 315 4.18e-68 220
MS.gene25352.t1 MTR_2g086020 50.000 156 73 3 45 198 1 153 2.15e-36 137
MS.gene25352.t1 MTR_8g014260 35.000 480 259 18 1 456 1 451 2.04e-66 220
MS.gene25352.t1 MTR_8g467800 35.477 482 254 15 8 460 4 457 9.37e-64 213
MS.gene25352.t1 MTR_8g468840 36.623 456 251 13 18 454 12 448 2.03e-62 209
MS.gene25352.t1 MTR_4g032730 33.538 489 258 14 8 455 4 466 6.27e-62 208
MS.gene25352.t1 MTR_5g092960 33.192 473 259 11 15 455 14 461 1.71e-58 199
MS.gene25352.t1 MTR_8g468760 34.461 473 226 15 12 455 1 418 6.87e-55 189
MS.gene25352.t1 MTR_8g468810 38.619 391 190 15 4 366 3 371 4.07e-53 182
MS.gene25352.t1 MTR_8g445780 36.207 406 215 15 20 409 13 390 8.30e-52 180
MS.gene25352.t1 MTR_8g468810 40.260 308 150 11 4 288 3 299 1.16e-44 159
MS.gene25352.t1 MTR_8g468810 40.260 308 150 11 4 288 3 299 1.59e-44 159
MS.gene25352.t1 MTR_8g468810 40.260 308 150 11 4 288 3 299 3.48e-44 158
MS.gene25352.t1 MTR_8g014160 30.853 457 224 14 20 456 41 425 4.38e-41 152
MS.gene25352.t1 MTR_5g092960 32.632 380 206 9 15 366 14 371 5.82e-38 143
MS.gene25352.t1 MTR_3g099800 70.115 87 25 1 368 453 19 105 1.13e-32 120
MS.gene25352.t1 MTR_8g467800 37.705 305 151 13 8 288 4 293 3.16e-32 125
MS.gene25352.t1 MTR_4g024710 39.500 200 104 6 258 456 164 347 8.28e-31 122
MS.gene25352.t1 MTR_8g077460 38.710 217 116 8 20 225 10 220 5.88e-30 120
MS.gene25352.t1 MTR_8g077460 41.722 151 80 5 309 456 230 375 4.15e-26 109
MS.gene25352.t1 MTR_5g021550 44.242 165 74 6 5 157 4 162 2.03e-28 110
MS.gene25352.t1 MTR_8g014210 38.500 200 102 7 260 456 50 231 2.24e-27 109
MS.gene25352.t1 MTR_1g041245 27.212 452 242 15 18 456 9 386 2.19e-25 107
MS.gene25352.t1 MTR_8g014290 39.306 173 77 7 1 158 1 160 6.65e-25 100
MS.gene25352.t1 MTR_7g026500 26.205 477 251 16 7 460 1 399 1.30e-23 102
MS.gene25352.t1 MTR_7g098510 26.170 470 254 19 1 456 1 391 2.84e-21 95.9
MS.gene25352.t1 MTR_4g024745 30.249 281 148 11 170 431 25 276 2.85e-20 90.9
MS.gene25352.t1 MTR_5g084070 26.490 453 285 18 8 448 3 419 1.25e-19 91.3
MS.gene25352.t1 MTR_4g024720 39.231 130 67 5 4 126 3 127 2.20e-18 84.0
MS.gene25352.t1 MTR_5g080710 27.191 445 262 18 3 428 24 425 3.33e-18 87.0
MS.gene25352.t1 MTR_5g073420 32.461 191 118 6 20 205 11 195 5.51e-17 82.8
MS.gene25352.t1 MTR_7g056447 32.821 195 120 6 12 204 1 186 9.72e-17 80.9
MS.gene25352.t1 MTR_7g098480 26.237 465 256 14 1 455 1 388 2.14e-16 81.6
MS.gene25352.t1 MTR_5g007410 25.459 436 263 12 22 453 28 405 9.40e-16 79.7
MS.gene25352.t1 MTR_1g108820 28.188 447 249 22 14 453 2 383 9.71e-16 79.0
MS.gene25352.t1 MTR_2g007400 24.839 467 252 18 17 456 9 403 1.05e-15 79.3
MS.gene25352.t1 MTR_2g008310 26.444 450 238 17 20 456 2 371 1.13e-15 79.0
MS.gene25352.t1 MTR_7g032380 35.385 195 107 7 20 207 11 193 1.28e-15 78.6
MS.gene25352.t1 MTR_1g053025 26.233 446 256 17 20 453 2 386 3.26e-15 77.4
MS.gene25352.t1 MTR_3g107120 32.836 201 113 10 20 205 9 202 9.48e-15 76.3
MS.gene25352.t1 MTR_4g024770 50.000 64 29 1 1 64 8 68 2.56e-14 68.2
MS.gene25352.t1 MTR_7g115270 30.137 219 125 8 8 213 5 208 2.80e-14 74.7
MS.gene25352.t1 MTR_5g008920 24.622 463 256 18 7 457 1 382 6.69e-14 73.6
MS.gene25352.t1 MTR_3g107100 30.049 203 129 9 10 208 2 195 7.75e-14 73.6
MS.gene25352.t1 MTR_2g008320 35.204 196 109 8 20 207 52 237 8.30e-14 73.6
MS.gene25352.t1 MTR_5g008950 29.524 210 133 7 22 230 1 196 1.19e-13 72.8
MS.gene25352.t1 MTR_2g007500 30.189 212 112 7 7 207 1 187 3.33e-13 70.9
MS.gene25352.t1 MTR_8g014180 55.172 58 24 1 4 61 3 58 4.47e-13 64.3
MS.gene25352.t1 MTR_3g036250 29.949 197 119 7 19 207 4 189 5.47e-13 70.5
MS.gene25352.t1 MTR_3g105060 31.980 197 122 9 20 213 14 201 6.06e-13 70.5
MS.gene25352.t1 MTR_1g492880 39.785 93 52 2 12 103 1 90 6.51e-13 65.1
MS.gene25352.t1 MTR_1g492880 39.785 93 52 2 12 103 1 90 8.14e-13 65.1
MS.gene25352.t1 MTR_7g034110 46.341 82 40 2 20 101 11 88 1.15e-12 69.3
MS.gene25352.t1 MTR_2g007450 30.357 224 132 8 9 211 5 225 1.24e-12 69.7
MS.gene25352.t1 MTR_4g098590 30.808 198 120 7 22 207 1 193 2.41e-12 68.6
MS.gene25352.t1 MTR_3g023630 30.964 197 111 8 19 205 7 188 2.99e-12 68.2
MS.gene25352.t1 MTR_3g040700 28.866 194 125 7 19 207 42 227 3.05e-12 68.6
MS.gene25352.t1 MTR_7g033990 30.000 210 117 7 17 212 8 201 3.12e-12 68.6
MS.gene25352.t1 MTR_7g056433 33.010 206 123 7 7 207 1 196 4.64e-12 67.8
MS.gene25352.t1 MTR_8g014230 40.000 85 47 2 365 449 18 98 5.84e-12 62.4
MS.gene25352.t1 MTR_8g085430 31.500 200 115 7 20 207 11 200 1.58e-11 65.9
MS.gene25352.t1 MTR_5g083890 31.884 207 110 7 20 203 6 204 1.86e-11 66.6
MS.gene25352.t1 MTR_7g055943 27.143 210 137 7 2 205 22 221 2.86e-11 65.5
MS.gene25352.t1 MTR_1g107385 31.441 229 135 10 3 216 20 241 4.96e-11 64.7
MS.gene25352.t1 MTR_3g467400 29.386 228 132 9 18 223 1 221 6.16e-11 64.3
MS.gene25352.t1 MTR_7g034115 32.474 194 118 6 18 207 9 193 7.60e-11 63.9
MS.gene25352.t1 MTR_7g033945 30.814 172 99 6 20 182 11 171 8.91e-11 61.2
MS.gene25352.t1 MTR_7g033940 33.858 127 74 5 15 139 89 207 9.26e-11 62.0
MS.gene25352.t1 MTR_4g051480 29.936 157 100 5 20 175 25 172 9.93e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25352.t1 AT4G10400 27.679 448 271 17 20 455 2 408 6.63e-22 97.8
MS.gene25352.t1 AT4G10400 27.679 448 271 17 20 455 2 408 6.63e-22 97.8
MS.gene25352.t1 AT4G10400 27.679 448 271 17 20 455 2 408 6.63e-22 97.8
MS.gene25352.t1 AT1G78750 26.415 477 301 20 4 453 2 455 1.32e-19 91.7
MS.gene25352.t1 AT1G78750 26.415 477 301 20 4 453 2 455 1.32e-19 91.7
MS.gene25352.t1 AT1G32375 27.119 472 258 19 20 456 2 422 3.99e-18 86.7
MS.gene25352.t1 AT1G32375 27.119 472 258 19 20 456 2 422 3.99e-18 86.7
MS.gene25352.t1 AT4G00160 28.139 462 282 18 20 461 16 447 5.15e-16 80.5
MS.gene25352.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.39e-15 77.8
MS.gene25352.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.39e-15 77.8
MS.gene25352.t1 AT5G50270 28.409 440 240 19 20 427 2 398 1.60e-15 78.6
MS.gene25352.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.94e-15 77.0
MS.gene25352.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.94e-15 77.0
MS.gene25352.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.94e-15 77.0
MS.gene25352.t1 AT3G50710 25.541 462 283 13 20 456 2 427 4.79e-14 74.3
MS.gene25352.t1 AT5G56440 34.392 189 113 4 20 205 2 182 9.93e-14 73.2
MS.gene25352.t1 AT4G26340 27.074 458 273 17 20 456 2 419 2.11e-13 72.4
MS.gene25352.t1 AT5G53840 24.775 444 245 17 20 426 18 409 3.62e-13 71.6
MS.gene25352.t1 AT3G59190 29.310 174 106 4 20 184 12 177 9.15e-13 70.5
MS.gene25352.t1 AT4G13965 27.115 461 261 18 19 453 9 420 1.20e-12 70.1
MS.gene25352.t1 AT3G59190 29.310 174 106 4 20 184 12 177 1.25e-12 69.7
MS.gene25352.t1 AT3G59200 30.151 199 114 7 20 205 7 193 2.48e-12 68.6
MS.gene25352.t1 AT4G13985 31.841 201 116 9 19 207 18 209 3.73e-12 68.6
MS.gene25352.t1 AT1G16930 29.126 206 127 7 8 207 3 195 4.91e-12 68.2
MS.gene25352.t1 AT1G16930 29.126 206 127 7 8 207 3 195 4.91e-12 68.2
MS.gene25352.t1 AT1G16930 29.126 206 127 7 8 207 3 195 4.91e-12 68.2
MS.gene25352.t1 AT3G59200 30.151 199 114 7 20 205 7 193 5.42e-12 68.2
MS.gene25352.t1 AT5G60610 24.941 421 257 19 20 420 2 383 5.74e-12 67.8
MS.gene25352.t1 AT5G60610 24.941 421 257 19 20 420 2 383 5.74e-12 67.8
MS.gene25352.t1 AT4G14103 31.282 195 115 5 20 205 8 192 6.02e-12 67.8
MS.gene25352.t1 AT4G14103 31.088 193 118 4 20 205 8 192 6.64e-12 67.4
MS.gene25352.t1 AT4G14103 31.088 193 118 4 20 205 8 192 6.64e-12 67.4
MS.gene25352.t1 AT1G48400 30.052 193 121 4 20 205 10 195 6.67e-12 67.8
MS.gene25352.t1 AT1G48400 30.052 193 121 4 20 205 10 195 8.71e-12 67.4
MS.gene25352.t1 AT3G52680 32.143 196 114 6 20 208 21 204 1.38e-11 66.6
MS.gene25352.t1 AT3G52680 32.143 196 114 6 20 208 21 204 2.74e-11 65.5

Find 111 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATAGTTTACCTTCTATT+TGG 0.229961 8.2:+39043268 MS.gene25352:CDS
CATTGGCAACTTTCACAGTA+TGG 0.259899 8.2:+39043468 MS.gene25352:CDS
AAAATTGTCATTGGCCGATT+TGG 0.299413 8.2:-39043389 None:intergenic
CAACTTAAATTTGACGTTGA+TGG 0.335217 8.2:+39044267 MS.gene25352:CDS
CTCGCCGGTGGCGCCACCTC+TGG 0.348002 8.2:+39042744 MS.gene25352:CDS
TGATGTAAACGGCTAAATTC+AGG 0.379198 8.2:-39043763 None:intergenic
GGAAGGTGGAATTAAAGCTT+TGG 0.380976 8.2:-39043350 None:intergenic
TGCATCAGATTGGGCATCAT+TGG 0.387142 8.2:+39043451 MS.gene25352:CDS
CTCGGATGAGGTTGAGAATA+GGG 0.388430 8.2:-39043540 None:intergenic
GACGTTGATGGGTCTCATTA+AGG 0.393555 8.2:+39044279 MS.gene25352:CDS
CTGACTTATGTTCACATAGA+AGG 0.406124 8.2:+39044081 MS.gene25352:CDS
AACTTAAATTTGACGTTGAT+GGG 0.410278 8.2:+39044268 MS.gene25352:CDS
GTAAAGGAGGTTGGAGCAAT+TGG 0.413248 8.2:-39043044 None:intergenic
TATGAATGTCGCGGAATTTG+CGG 0.415114 8.2:-39042859 None:intergenic
TAGATTCACATTCGTTCAAA+AGG 0.416585 8.2:+39042804 MS.gene25352:CDS
AAATAATGAATGCATCAGAT+TGG 0.421459 8.2:+39043441 MS.gene25352:CDS
GAGATAGAGTTGTTGAAGGT+GGG 0.425273 8.2:-39042975 None:intergenic
ACTTGGGACTGCCAAATAGA+AGG 0.427636 8.2:-39043279 None:intergenic
CGATTTGGAGCTCTCGGAGC+TGG 0.442706 8.2:-39043374 None:intergenic
CTTTCCAGAGGTGGCGCCAC+CGG 0.463852 8.2:-39042748 None:intergenic
AATAATGAATGCATCAGATT+GGG 0.466409 8.2:+39043442 MS.gene25352:CDS
AGTCCAAGTAAAATTGTCAT+TGG 0.468154 8.2:-39043398 None:intergenic
CATCATAGCGACGGAGGTTG+TGG 0.470605 8.2:-39042714 None:intergenic
AACCCGCAAAACTTCCAACT+TGG 0.473471 8.2:-39043296 None:intergenic
CGCGGAATTTGCGGAGGGCG+AGG 0.475468 8.2:-39042850 None:intergenic
GCTCCAACCTCCTTTACCTC+AGG 0.478577 8.2:+39043050 MS.gene25352:CDS
TATACATCATAGCAGTCAGC+TGG 0.478695 8.2:-39043595 None:intergenic
TCTCGGATGAGGTTGAGAAT+AGG 0.481952 8.2:-39043541 None:intergenic
GAAGGTGGAATTAAAGCTTT+GGG 0.484659 8.2:-39043349 None:intergenic
GCAATTATAACCTGAGGTAA+AGG 0.484740 8.2:-39043060 None:intergenic
CATCAACGTCAAATTTAAGT+TGG 0.486323 8.2:-39044266 None:intergenic
TGTAGGTTCTTATAGTATAG+AGG 0.487842 8.2:+39043174 MS.gene25352:CDS
CTCAACCTCATCCGAGATGA+TGG 0.488519 8.2:+39043547 MS.gene25352:CDS
TACTCGGCTTTAATCGATCC+GGG 0.489291 8.2:+39042894 MS.gene25352:CDS
ACGTGGATCCTTGCTGCCAT+TGG 0.491503 8.2:+39042950 MS.gene25352:CDS
ATGTTTCGACTCAAATCTGC+TGG 0.494251 8.2:+39043804 MS.gene25352:CDS
CATAAGTTCCATCTCATACT+CGG 0.496407 8.2:+39042878 MS.gene25352:CDS
CTTCTATGTGAACATAAGTC+AGG 0.496958 8.2:-39044080 None:intergenic
ATACTCGGCTTTAATCGATC+CGG 0.499361 8.2:+39042893 MS.gene25352:CDS
GCCATTGAGAACATGGCAGC+TGG 0.499987 8.2:+39044023 MS.gene25352:CDS
ATTGGCAACTTTCACAGTAT+GGG 0.502787 8.2:+39043469 MS.gene25352:CDS
TGAACTTCAGATGATGTAAA+CGG 0.503521 8.2:-39043774 None:intergenic
GACAAATTCACTTTCAGTCA+AGG 0.506419 8.2:-39043515 None:intergenic
CAATTGGTCAAGAGGGAAGG+AGG 0.511905 8.2:-39043028 None:intergenic
TGCCATGTTCTCAATGGCAG+TGG 0.512316 8.2:-39044018 None:intergenic
TTCTGCCATCATCTCGGATG+AGG 0.513011 8.2:-39043552 None:intergenic
ATTGGCCGATTTGGAGCTCT+CGG 0.514913 8.2:-39043380 None:intergenic
TTGTTGAAGGTGGGGTCCAA+TGG 0.515637 8.2:-39042966 None:intergenic
TCCAGCTGCCATGTTCTCAA+TGG 0.515812 8.2:-39044024 None:intergenic
GTTCACATAGAAGGATATCA+AGG 0.522561 8.2:+39044090 MS.gene25352:CDS
TAACCTGAGGTAAAGGAGGT+TGG 0.524882 8.2:-39043053 None:intergenic
TGTAGAGATAGAGTTGTTGA+AGG 0.526137 8.2:-39042979 None:intergenic
CAGCTCCGAGAGCTCCAAAT+CGG 0.527982 8.2:+39043375 MS.gene25352:CDS
GAGCTCTCGGAGCTGGAGGA+AGG 0.528347 8.2:-39043367 None:intergenic
AGAGATAGAGTTGTTGAAGG+TGG 0.529187 8.2:-39042976 None:intergenic
AAGATTAACGAATATATCAA+AGG 0.534259 8.2:+39044230 MS.gene25352:CDS
TTGGCAACTTTCACAGTATG+GGG 0.536652 8.2:+39043470 MS.gene25352:CDS
TGATAGTTCAATAGACAAAA+CGG 0.539124 8.2:+39044188 MS.gene25352:CDS
ATGAAAGTTGTCATAAATCC+CGG 0.539720 8.2:-39042912 None:intergenic
GGAGGTTGTGGTAGGGAGGA+AGG 0.546045 8.2:-39042702 None:intergenic
AGATTAACGAATATATCAAA+GGG 0.548268 8.2:+39044231 MS.gene25352:CDS
TCTATTTGGCAGTCCCAAGT+TGG 0.550189 8.2:+39043282 MS.gene25352:CDS
AGCAGATTTGAGTCGAAACA+TGG 0.553943 8.2:-39043802 None:intergenic
TGTGGCATTGCTCAAGAACA+TGG 0.554101 8.2:-39043828 None:intergenic
CTCTCGGAGCTGGAGGAAGG+TGG 0.556854 8.2:-39043364 None:intergenic
AACCACTGCCATTGAGAACA+TGG 0.558537 8.2:+39044016 MS.gene25352:CDS
ATATGACACAGGATCGAGTC+CGG 0.559164 8.2:-39042674 None:intergenic
AACCTACTCGACGATGGCAA+CGG 0.559988 8.2:+39042586 None:intergenic
TTCAGGATAATTTAGAACAC+GGG 0.564152 8.2:-39043746 None:intergenic
AATTGGTCAAGAGGGAAGGA+GGG 0.570036 8.2:-39043027 None:intergenic
ACCCGCAAAACTTCCAACTT+GGG 0.570628 8.2:-39043295 None:intergenic
TTCAATAGACAAAACGGACA+TGG 0.570795 8.2:+39044194 MS.gene25352:CDS
GGACAGATTGAGCAGTCTAC+CGG 0.570908 8.2:+39042655 MS.gene25352:CDS
ATAGCGACGGAGGTTGTGGT+AGG 0.574834 8.2:-39042710 None:intergenic
AGATAGAGTTGTTGAAGGTG+GGG 0.580102 8.2:-39042974 None:intergenic
GATTAAAGCCGAGTATGAGA+TGG 0.586475 8.2:-39042886 None:intergenic
AGAGACAGCACTTTGAGCAA+TGG 0.588422 8.2:-39043220 None:intergenic
ATTCAGTTCTGCCATCATCT+CGG 0.593799 8.2:-39043558 None:intergenic
GAGCAATTGGTCAAGAGGGA+AGG 0.596231 8.2:-39043031 None:intergenic
GGTTGGAGCAATTGGTCAAG+AGG 0.598188 8.2:-39043036 None:intergenic
ATCTCGCTTGTATTCGTACG+TGG 0.599453 8.2:+39042933 MS.gene25352:CDS
GTTGCGTCATTCAACATCAA+AGG 0.603041 8.2:+39043624 MS.gene25352:CDS
CTATGTGAACATAAGTCAGG+TGG 0.604289 8.2:-39044077 None:intergenic
GAATGTCGCGGAATTTGCGG+AGG 0.613849 8.2:-39042856 None:intergenic
GATGGCAACGGAGTCAAGCA+TGG 0.614265 8.2:+39042598 None:intergenic
TAGCGACGGAGGTTGTGGTA+GGG 0.614525 8.2:-39042709 None:intergenic
TAATTTAGAACACGGGCATG+AGG 0.618358 8.2:-39043739 None:intergenic
GTTGGAGCAATTGGTCAAGA+GGG 0.618704 8.2:-39043035 None:intergenic
CGACGGAGGTTGTGGTAGGG+AGG 0.621858 8.2:-39042706 None:intergenic
GGTGGGGTCCAATGGCAGCA+AGG 0.624675 8.2:-39042958 None:intergenic
ATTATAACCTGAGGTAAAGG+AGG 0.629004 8.2:-39043057 None:intergenic
ATTCAGGATAATTTAGAACA+CGG 0.629666 8.2:-39043747 None:intergenic
TGATGAGAGTCTCTCGCCGG+TGG 0.630142 8.2:+39042732 MS.gene25352:CDS
TCTGCAGATCTTTCCAGAGG+TGG 0.633206 8.2:-39042757 None:intergenic
TTTGAGCAATGGAAAATGAA+CGG 0.634422 8.2:-39043209 None:intergenic
TTTGGAGCTCTCGGAGCTGG+AGG 0.636682 8.2:-39043371 None:intergenic
TATGTGAACATAAGTCAGGT+GGG 0.640734 8.2:-39044076 None:intergenic
CTATGATGAGAGTCTCTCGC+CGG 0.640993 8.2:+39042729 MS.gene25352:CDS
ATACATCATAGCAGTCAGCT+GGG 0.647711 8.2:-39043594 None:intergenic
TCTCATAATTCAGAGCAACA+AGG 0.652411 8.2:+39043861 MS.gene25352:CDS
GGAAGGACATGATATGACAC+AGG 0.667460 8.2:-39042685 None:intergenic
AGAGACTCTCATCATAGCGA+CGG 0.669339 8.2:-39042723 None:intergenic
ATATCTGCAGATCTTTCCAG+AGG 0.674707 8.2:-39042760 None:intergenic
TGGCAACTTTCACAGTATGG+GGG 0.681502 8.2:+39043471 MS.gene25352:CDS
AATGTCGCGGAATTTGCGGA+GGG 0.681823 8.2:-39042855 None:intergenic
GATGGAACTTATGAATGTCG+CGG 0.686296 8.2:-39042868 None:intergenic
GACTCTCATCATAGCGACGG+AGG 0.689212 8.2:-39042720 None:intergenic
TTATGAGAACTTGAAGCATG+TGG 0.695487 8.2:-39043846 None:intergenic
TGCAGAAGAAGACAACGTCA+CGG 0.699654 8.2:+39042634 MS.gene25352:CDS
ACTGCTATGATGTATATCTG+CGG 0.701238 8.2:+39043602 MS.gene25352:CDS
CATCATAGCAGTCAGCTGGG+AGG 0.736987 8.2:-39043591 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATTAACGAATATATCAA+AGG + chr8.2:39044230-39044249 MS.gene25352:CDS 20.0%
!! ACATACATACATGTATATAT+AGG - chr8.2:39043929-39043948 None:intergenic 20.0%
!! AGATTAACGAATATATCAAA+GGG + chr8.2:39044231-39044250 MS.gene25352:CDS 20.0%
!! AGTTTAATACAAACAATCTT+TGG + chr8.2:39043899-39043918 MS.gene25352:intron 20.0%
!! ATGGTTTATATGTATATCTA+CGG + chr8.2:39043662-39043681 MS.gene25352:intron 20.0%
!! GTCATTACTCAAAAATATAT+TGG - chr8.2:39043969-39043988 None:intergenic 20.0%
!!! TATATTTTTGAGTAATGACA+TGG + chr8.2:39043970-39043989 MS.gene25352:intron 20.0%
! AAATAATGAATGCATCAGAT+TGG + chr8.2:39043441-39043460 MS.gene25352:CDS 25.0%
! AACTTAAATTTGACGTTGAT+GGG + chr8.2:39044268-39044287 MS.gene25352:CDS 25.0%
! AATAATGAATGCATCAGATT+GGG + chr8.2:39043442-39043461 MS.gene25352:CDS 25.0%
! GAAATAGTTTACCTTCTATT+TGG + chr8.2:39043268-39043287 MS.gene25352:CDS 25.0%
! TAAAAAGCAATTATAACCTG+AGG - chr8.2:39043069-39043088 None:intergenic 25.0%
! TGATAGTTCAATAGACAAAA+CGG + chr8.2:39044188-39044207 MS.gene25352:CDS 25.0%
! TTATATGTATATCTACGGTT+TGG + chr8.2:39043667-39043686 MS.gene25352:intron 25.0%
!! ATTCAGGATAATTTAGAACA+CGG - chr8.2:39043750-39043769 None:intergenic 25.0%
!!! AAGGTGATGTTTTATTCTTA+TGG + chr8.2:39043643-39043662 MS.gene25352:intron 25.0%
!!! CATTTGCTGAATATATTTTG+CGG + chr8.2:39044130-39044149 MS.gene25352:CDS 25.0%
AGTCCAAGTAAAATTGTCAT+TGG - chr8.2:39043401-39043420 None:intergenic 30.0%
ATGAAAGTTGTCATAAATCC+CGG - chr8.2:39042915-39042934 None:intergenic 30.0%
CAACTTAAATTTGACGTTGA+TGG + chr8.2:39044267-39044286 MS.gene25352:CDS 30.0%
CATCAACGTCAAATTTAAGT+TGG - chr8.2:39044269-39044288 None:intergenic 30.0%
GAAATGTTAGAAGTGAGTTT+GGG - chr8.2:39043094-39043113 None:intergenic 30.0%
TAGATTCACATTCGTTCAAA+AGG + chr8.2:39042804-39042823 MS.gene25352:CDS 30.0%
TGAAATGTTAGAAGTGAGTT+TGG - chr8.2:39043095-39043114 None:intergenic 30.0%
TGAACTTCAGATGATGTAAA+CGG - chr8.2:39043777-39043796 None:intergenic 30.0%
TGTAGGTTCTTATAGTATAG+AGG + chr8.2:39043174-39043193 MS.gene25352:CDS 30.0%
TTTGAGCAATGGAAAATGAA+CGG - chr8.2:39043212-39043231 None:intergenic 30.0%
! TTCAGGATAATTTAGAACAC+GGG - chr8.2:39043749-39043768 None:intergenic 30.0%
! TTTTAGACTCACAATTTCTG+CGG - chr8.2:39043248-39043267 None:intergenic 30.0%
!! TCTTTTGAAAACAGTCTTGT+AGG + chr8.2:39043157-39043176 MS.gene25352:intron 30.0%
AAAATTGTCATTGGCCGATT+TGG - chr8.2:39043392-39043411 None:intergenic 35.0%
ACTGCTATGATGTATATCTG+CGG + chr8.2:39043602-39043621 MS.gene25352:CDS 35.0%
ATTATAACCTGAGGTAAAGG+AGG - chr8.2:39043060-39043079 None:intergenic 35.0%
ATTGGCAACTTTCACAGTAT+GGG + chr8.2:39043469-39043488 MS.gene25352:CDS 35.0%
CATAAGTTCCATCTCATACT+CGG + chr8.2:39042878-39042897 MS.gene25352:CDS 35.0%
CTGACTTATGTTCACATAGA+AGG + chr8.2:39044081-39044100 MS.gene25352:CDS 35.0%
CTTCTATGTGAACATAAGTC+AGG - chr8.2:39044083-39044102 None:intergenic 35.0%
GAAGGTGGAATTAAAGCTTT+GGG - chr8.2:39043352-39043371 None:intergenic 35.0%
GACAAATTCACTTTCAGTCA+AGG - chr8.2:39043518-39043537 None:intergenic 35.0%
GCAATTATAACCTGAGGTAA+AGG - chr8.2:39043063-39043082 None:intergenic 35.0%
GTTCACATAGAAGGATATCA+AGG + chr8.2:39044090-39044109 MS.gene25352:CDS 35.0%
TATGTGAACATAAGTCAGGT+GGG - chr8.2:39044079-39044098 None:intergenic 35.0%
TCTCATAATTCAGAGCAACA+AGG + chr8.2:39043861-39043880 MS.gene25352:CDS 35.0%
TGATGTAAACGGCTAAATTC+AGG - chr8.2:39043766-39043785 None:intergenic 35.0%
TGTAGAGATAGAGTTGTTGA+AGG - chr8.2:39042982-39043001 None:intergenic 35.0%
TTATGAGAACTTGAAGCATG+TGG - chr8.2:39043849-39043868 None:intergenic 35.0%
TTCAATAGACAAAACGGACA+TGG + chr8.2:39044194-39044213 MS.gene25352:CDS 35.0%
! GCTGAATATATTTTGCGGAA+TGG + chr8.2:39044135-39044154 MS.gene25352:CDS 35.0%
AATGACATGGTTTCCCTTGT+TGG + chr8.2:39043983-39044002 MS.gene25352:intron 40.0%
AGAGATAGAGTTGTTGAAGG+TGG - chr8.2:39042979-39042998 None:intergenic 40.0%
AGATAGAGTTGTTGAAGGTG+GGG - chr8.2:39042977-39042996 None:intergenic 40.0%
AGCAGATTTGAGTCGAAACA+TGG - chr8.2:39043805-39043824 None:intergenic 40.0%
ATACATCATAGCAGTCAGCT+GGG - chr8.2:39043597-39043616 None:intergenic 40.0%
ATATCTGCAGATCTTTCCAG+AGG - chr8.2:39042763-39042782 None:intergenic 40.0%
ATGTTTCGACTCAAATCTGC+TGG + chr8.2:39043804-39043823 MS.gene25352:CDS 40.0%
ATTCAGTTCTGCCATCATCT+CGG - chr8.2:39043561-39043580 None:intergenic 40.0%
CATTGGCAACTTTCACAGTA+TGG + chr8.2:39043468-39043487 MS.gene25352:CDS 40.0%
CTATGTGAACATAAGTCAGG+TGG - chr8.2:39044080-39044099 None:intergenic 40.0%
GAGATAGAGTTGTTGAAGGT+GGG - chr8.2:39042978-39042997 None:intergenic 40.0%
GATGGAACTTATGAATGTCG+CGG - chr8.2:39042871-39042890 None:intergenic 40.0%
GATTAAAGCCGAGTATGAGA+TGG - chr8.2:39042889-39042908 None:intergenic 40.0%
GGAAGGTGGAATTAAAGCTT+TGG - chr8.2:39043353-39043372 None:intergenic 40.0%
GTTGCGTCATTCAACATCAA+AGG + chr8.2:39043624-39043643 MS.gene25352:CDS 40.0%
TATACATCATAGCAGTCAGC+TGG - chr8.2:39043598-39043617 None:intergenic 40.0%
TATGAATGTCGCGGAATTTG+CGG - chr8.2:39042862-39042881 None:intergenic 40.0%
TTGGCAACTTTCACAGTATG+GGG + chr8.2:39043470-39043489 MS.gene25352:CDS 40.0%
! ATACTCGGCTTTAATCGATC+CGG + chr8.2:39042893-39042912 MS.gene25352:CDS 40.0%
! CGGCCAATGACAATTTTACT+TGG + chr8.2:39043395-39043414 MS.gene25352:CDS 40.0%
! TAATTTAGAACACGGGCATG+AGG - chr8.2:39043742-39043761 None:intergenic 40.0%
AACCACTGCCATTGAGAACA+TGG + chr8.2:39044016-39044035 MS.gene25352:CDS 45.0%
AACCCGCAAAACTTCCAACT+TGG - chr8.2:39043299-39043318 None:intergenic 45.0%
AATTGGTCAAGAGGGAAGGA+GGG - chr8.2:39043030-39043049 None:intergenic 45.0%
ACCCGCAAAACTTCCAACTT+GGG - chr8.2:39043298-39043317 None:intergenic 45.0%
ACTTGGGACTGCCAAATAGA+AGG - chr8.2:39043282-39043301 None:intergenic 45.0%
AGAGACAGCACTTTGAGCAA+TGG - chr8.2:39043223-39043242 None:intergenic 45.0%
AGAGACTCTCATCATAGCGA+CGG - chr8.2:39042726-39042745 None:intergenic 45.0%
ATATGACACAGGATCGAGTC+CGG - chr8.2:39042677-39042696 None:intergenic 45.0%
ATCTCGCTTGTATTCGTACG+TGG + chr8.2:39042933-39042952 MS.gene25352:CDS 45.0%
CTCGGATGAGGTTGAGAATA+GGG - chr8.2:39043543-39043562 None:intergenic 45.0%
GGAAGGACATGATATGACAC+AGG - chr8.2:39042688-39042707 None:intergenic 45.0%
GTAAAGGAGGTTGGAGCAAT+TGG - chr8.2:39043047-39043066 None:intergenic 45.0%
GTTGGAGCAATTGGTCAAGA+GGG - chr8.2:39043038-39043057 None:intergenic 45.0%
TAACCTGAGGTAAAGGAGGT+TGG - chr8.2:39043056-39043075 None:intergenic 45.0%
TCTATTTGGCAGTCCCAAGT+TGG + chr8.2:39043282-39043301 MS.gene25352:CDS 45.0%
TCTCGGATGAGGTTGAGAAT+AGG - chr8.2:39043544-39043563 None:intergenic 45.0%
TGCAGAAGAAGACAACGTCA+CGG + chr8.2:39042634-39042653 MS.gene25352:CDS 45.0%
TGCATCAGATTGGGCATCAT+TGG + chr8.2:39043451-39043470 MS.gene25352:CDS 45.0%
TGGCAACTTTCACAGTATGG+GGG + chr8.2:39043471-39043490 MS.gene25352:CDS 45.0%
TGTGGCATTGCTCAAGAACA+TGG - chr8.2:39043831-39043850 None:intergenic 45.0%
TTGTTATCGTGTACCTGCAG+TGG + chr8.2:39043713-39043732 MS.gene25352:intron 45.0%
! TACTCGGCTTTAATCGATCC+GGG + chr8.2:39042894-39042913 MS.gene25352:CDS 45.0%
!! GTCCCAAGTTGGAAGTTTTG+CGG + chr8.2:39043293-39043312 MS.gene25352:CDS 45.0%
!! TCCCAAGTTGGAAGTTTTGC+GGG + chr8.2:39043294-39043313 MS.gene25352:CDS 45.0%
AATGTCGCGGAATTTGCGGA+GGG - chr8.2:39042858-39042877 None:intergenic 50.0%
CAATTGGTCAAGAGGGAAGG+AGG - chr8.2:39043031-39043050 None:intergenic 50.0%
CTATGATGAGAGTCTCTCGC+CGG + chr8.2:39042729-39042748 MS.gene25352:CDS 50.0%
CTCAACCTCATCCGAGATGA+TGG + chr8.2:39043547-39043566 MS.gene25352:CDS 50.0%
GACATGGTTTCCCTTGTTGG+TGG + chr8.2:39043986-39044005 MS.gene25352:intron 50.0%
GAGCAATTGGTCAAGAGGGA+AGG - chr8.2:39043034-39043053 None:intergenic 50.0%
GGACAGATTGAGCAGTCTAC+CGG + chr8.2:39042655-39042674 MS.gene25352:CDS 50.0%
GGTTGGAGCAATTGGTCAAG+AGG - chr8.2:39043039-39043058 None:intergenic 50.0%
GTTCCTCCTACCACCAACAA+GGG - chr8.2:39043999-39044018 None:intergenic 50.0%
TCCAGCTGCCATGTTCTCAA+TGG - chr8.2:39044027-39044046 None:intergenic 50.0%
TCTGCAGATCTTTCCAGAGG+TGG - chr8.2:39042760-39042779 None:intergenic 50.0%
TGCCATGTTCTCAATGGCAG+TGG - chr8.2:39044021-39044040 None:intergenic 50.0%
TTCTGCCATCATCTCGGATG+AGG - chr8.2:39043555-39043574 None:intergenic 50.0%
TTGTTGAAGGTGGGGTCCAA+TGG - chr8.2:39042969-39042988 None:intergenic 50.0%
!! ATTGGCCGATTTGGAGCTCT+CGG - chr8.2:39043383-39043402 None:intergenic 50.0%
!! TGGTTTCCCTTGTTGGTGGT+AGG + chr8.2:39043990-39044009 MS.gene25352:intron 50.0%
!! TTTCCCTTGTTGGTGGTAGG+AGG + chr8.2:39043993-39044012 MS.gene25352:intron 50.0%
ACGTGGATCCTTGCTGCCAT+TGG + chr8.2:39042950-39042969 MS.gene25352:CDS 55.0%
ATAGCGACGGAGGTTGTGGT+AGG - chr8.2:39042713-39042732 None:intergenic 55.0%
CAGCTCCGAGAGCTCCAAAT+CGG + chr8.2:39043375-39043394 MS.gene25352:CDS 55.0%
CATCATAGCAGTCAGCTGGG+AGG - chr8.2:39043594-39043613 None:intergenic 55.0%
CATCATAGCGACGGAGGTTG+TGG - chr8.2:39042717-39042736 None:intergenic 55.0%
GAATGTCGCGGAATTTGCGG+AGG - chr8.2:39042859-39042878 None:intergenic 55.0%
GACTCTCATCATAGCGACGG+AGG - chr8.2:39042723-39042742 None:intergenic 55.0%
GCCATTGAGAACATGGCAGC+TGG + chr8.2:39044023-39044042 MS.gene25352:CDS 55.0%
GCTCCAACCTCCTTTACCTC+AGG + chr8.2:39043050-39043069 MS.gene25352:CDS 55.0%
GGTTCCTCCTACCACCAACA+AGG - chr8.2:39044000-39044019 None:intergenic 55.0%
TAGCGACGGAGGTTGTGGTA+GGG - chr8.2:39042712-39042731 None:intergenic 55.0%
GGAGGTTGTGGTAGGGAGGA+AGG - chr8.2:39042705-39042724 None:intergenic 60.0%
TGATGAGAGTCTCTCGCCGG+TGG + chr8.2:39042732-39042751 MS.gene25352:CDS 60.0%
! CGATTTGGAGCTCTCGGAGC+TGG - chr8.2:39043377-39043396 None:intergenic 60.0%
! TTTGGAGCTCTCGGAGCTGG+AGG - chr8.2:39043374-39043393 None:intergenic 60.0%
CGACGGAGGTTGTGGTAGGG+AGG - chr8.2:39042709-39042728 None:intergenic 65.0%
CTCTCGGAGCTGGAGGAAGG+TGG - chr8.2:39043367-39043386 None:intergenic 65.0%
CTTTCCAGAGGTGGCGCCAC+CGG - chr8.2:39042751-39042770 None:intergenic 65.0%
GAGCTCTCGGAGCTGGAGGA+AGG - chr8.2:39043370-39043389 None:intergenic 65.0%
GGTGGGGTCCAATGGCAGCA+AGG - chr8.2:39042961-39042980 None:intergenic 65.0%
CGCGGAATTTGCGGAGGGCG+AGG - chr8.2:39042853-39042872 None:intergenic 70.0%
GGGCATGAGGTCGCCACTGC+AGG - chr8.2:39043729-39043748 None:intergenic 70.0%
! CTCGCCGGTGGCGCCACCTC+TGG + chr8.2:39042744-39042763 MS.gene25352:CDS 80.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 39042599 39044299 39042599 ID=MS.gene25352
chr8.2 mRNA 39042599 39044299 39042599 ID=MS.gene25352.t1;Parent=MS.gene25352
chr8.2 exon 39042599 39043071 39042599 ID=MS.gene25352.t1.exon1;Parent=MS.gene25352.t1
chr8.2 CDS 39042599 39043071 39042599 ID=cds.MS.gene25352.t1;Parent=MS.gene25352.t1
chr8.2 exon 39043171 39043645 39043171 ID=MS.gene25352.t1.exon2;Parent=MS.gene25352.t1
chr8.2 CDS 39043171 39043645 39043171 ID=cds.MS.gene25352.t1;Parent=MS.gene25352.t1
chr8.2 exon 39043733 39043882 39043733 ID=MS.gene25352.t1.exon3;Parent=MS.gene25352.t1
chr8.2 CDS 39043733 39043882 39043733 ID=cds.MS.gene25352.t1;Parent=MS.gene25352.t1
chr8.2 exon 39044012 39044299 39044012 ID=MS.gene25352.t1.exon4;Parent=MS.gene25352.t1
chr8.2 CDS 39044012 39044299 39044012 ID=cds.MS.gene25352.t1;Parent=MS.gene25352.t1
Gene Sequence

>MS.gene25352

ATGGCAACGGAGTCAAGCATGGAACGATCGCTTGTTGCAGAAGAAGACAACGTCACGGACAGATTGAGCAGTCTACCGGACTCGATCCTGTGTCATATCATGTCCTTCCTCCCTACCACAACCTCCGTCGCTATGATGAGAGTCTCTCGCCGGTGGCGCCACCTCTGGAAAGATCTGCAGATATTCAACTTCTGCAGTCGCAATCTAGATTCACATTCGTTCAAAAGGTTTTTATTATTCGTGAACGCTGTCCTCGCCCTCCGCAAATTCCGCGACATTCATAAGTTCCATCTCATACTCGGCTTTAATCGATCCGGGATTTATGACAACTTTCATCTCGCTTGTATTCGTACGTGGATCCTTGCTGCCATTGGACCCCACCTTCAACAACTCTATCTCTACATTGTCAATGCTGCTGTTGTAGTTCTCCCTCCTTCCCTCTTGACCAATTGCTCCAACCTCCTTTACCTCAGGTTATAATTGCTTTTTATGCCCAAACTCACTTCTAACATTTCATTTCTTGTATTTTTACTTTTTTTTTTCTTTCTAACATGTTTGTCTTTTGAAAACAGTCTTGTAGGTTCTTATAGTATAGAGGTAAAACAGTCTTCCGTTCATTTTCCATTGCTCAAAGTGCTGTCTCTCGCCGCAGAAATTGTGAGTCTAAAAGAAATAGTTTACCTTCTATTTGGCAGTCCCAAGTTGGAAGTTTTGCGGGTTTACTTTTACTTTATCTCTGAAGAACTTGTGCCCAAAGCTTTAATTCCACCTTCCTCCAGCTCCGAGAGCTCCAAATCGGCCAATGACAATTTTACTTGGACTTACTTTAAAATAGTTAACACAAATAATGAATGCATCAGATTGGGCATCATTGGCAACTTTCACAGTATGGGGGAAGCATTTCTTGATGTCTTTTCCTTGACTGAAAGTGAATTTGTCGACCCTATTCTCAACCTCATCCGAGATGATGGCAGAACTGAATTTTATAAAAACCTCCCAGCTGACTGCTATGATGTATATCTGCGGTTGCGTCATTCAACATCAAAGGTGATGTTTTATTCTTATGGTTTATATGTATATCTACGGTTTGGTACTAATAGTGCGTCATCACTTGTTGTTATCGTGTACCTGCAGTGGCGACCTCATGCCCGTGTTCTAAATTATCCTGAATTTAGCCGTTTACATCATCTGAAGTTCATTCTTCCATGTTTCGACTCAAATCTGCTGGTCCATGTTCTTGAGCAATGCCACATGCTTCAAGTTCTCATAATTCAGAGCAACAAGGTTCGTTGACTTCTCAAGTTTAATACAAACAATCTTTGGTCTGCCTATATATACATGTATGTATGTATGTATCTATCTGTACACCAATATATTTTTGAGTAATGACATGGTTTCCCTTGTTGGTGGTAGGAGGAACCACTGCCATTGAGAACATGGCAGCTGGAGTCAACAACAGTTTTTGAATGTCTAAAATCCCACCTGACTTATGTTCACATAGAAGGATATCAAGGACTTGAAGATGAGTTCACATTTGCTGAATATATTTTGCGGAATGGACTTCTTTTGAAGACGATGCTTATTTTCGTTGATAGTTCAATAGACAAAACGGACATGGATTGTTCTCTCAAAAGATTAACGAATATATCAAAGGGCTCTGTTGCTTGCCAACTTAAATTTGACGTTGATGGGTCTCATTAA
Protein sequence

>MS.gene25352.t1

MATESSMERSLVAEEDNVTDRLSSLPDSILCHIMSFLPTTTSVAMMRVSRRWRHLWKDLQIFNFCSRNLDSHSFKRFLLFVNAVLALRKFRDIHKFHLILGFNRSGIYDNFHLACIRTWILAAIGPHLQQLYLYIVNAAVVVLPPSLLTNCSNLLYLSLVGSYSIEVKQSSVHFPLLKVLSLAAEIVSLKEIVYLLFGSPKLEVLRVYFYFISEELVPKALIPPSSSSESSKSANDNFTWTYFKIVNTNNECIRLGIIGNFHSMGEAFLDVFSLTESEFVDPILNLIRDDGRTEFYKNLPADCYDVYLRLRHSTSKWRPHARVLNYPEFSRLHHLKFILPCFDSNLLVHVLEQCHMLQVLIIQSNKEEPLPLRTWQLESTTVFECLKSHLTYVHIEGYQGLEDEFTFAEYILRNGLLLKTMLIFVDSSIDKTDMDCSLKRLTNISKGSVACQLKFDVDGSH