AlfalfaGEDB | MS.gene25450

M. sativa cultivar XinJiangDaYe / MS.gene25450

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00646.36 PF08387.13

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25450.t1	XP_013446248.1	47.9	428	84	10	31	322	15	439	2.60E-83	318.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25450.t1	Q3E8L3	22.3	354	200	11	31	320	2	344	1.6e-07	58.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25450.t1	A0A072U383	47.9	428	84	10	31	322	15	439	1.9e-83	318.9

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene002112	MS.gene25450	PPI
MS.gene003456	MS.gene25450	PPI
MS.gene004045	MS.gene25450	PPI
MS.gene005794	MS.gene25450	PPI
MS.gene005795	MS.gene25450	PPI
MS.gene006692	MS.gene25450	PPI
MS.gene006725	MS.gene25450	PPI
MS.gene006727	MS.gene25450	PPI
MS.gene006728	MS.gene25450	PPI
MS.gene006730	MS.gene25450	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25450.t1	MTR_8g070950	72.727	198	50	4	128	322	243	439	1.21e-90	277
MS.gene25450.t1	MTR_8g070950	51.562	128	36	4	1	120	1	110	1.98e-22	97.4
MS.gene25450.t1	MTR_8g062010	66.502	203	66	2	120	321	233	434	4.89e-85	263
MS.gene25450.t1	MTR_8g062010	58.621	87	28	2	31	111	9	93	1.15e-20	92.4
MS.gene25450.t1	MTR_8g469020	62.312	199	72	3	125	322	260	456	9.88e-82	255
MS.gene25450.t1	MTR_8g469020	54.118	85	32	2	30	109	6	88	1.10e-16	80.9
MS.gene25450.t1	MTR_8g062300	61.321	212	66	2	126	321	270	481	8.24e-80	251
MS.gene25450.t1	MTR_8g062300	53.982	113	42	4	1	111	1	105	9.66e-23	98.6
MS.gene25450.t1	MTR_8g062310	63.265	196	70	2	128	322	239	433	4.51e-78	245
MS.gene25450.t1	MTR_8g062310	60.759	79	28	2	31	109	25	100	7.02e-21	92.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 77 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTTCATATTTGTAATCT+CGG	0.433276	8.2:+40997943	None:intergenic
AACCACCACCATTGAGAACA+TGG	0.560977	8.2:-40997493	MS.gene25450:CDS
AACGATGGCAGAGTCAAGCG+TGG	0.660565	8.2:-40998601	None:intergenic
AACTCTGCTATAACTCCTTA+AGG	0.444957	8.2:-40997233	None:intergenic
AACTCTGGATAACTTAAAAC+AGG	0.359210	8.2:+40997750	None:intergenic
AAGACTCATAGTCCTCACAG+AGG	0.808089	8.2:+40998437	None:intergenic
AAGATTATCTGAGATACCAA+TGG	0.557854	8.2:-40997282	MS.gene25450:CDS
AATGATGCCCAATGTGACAC+GGG	0.545808	8.2:+40998052	None:intergenic
AATGGTCGGACTCGGGAGGA+AGG	0.534339	8.2:+40998461	None:intergenic
AATGTTGGGGAATCTGCGGA+GGG	0.668481	8.2:+40998302	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTCTGGATAACTTAAAAC+AGG	+	chr8.2:40998085-40998104	None:intergenic	30.0%
ACTCTGGATAACTTAAAACA+GGG	+	chr8.2:40998084-40998103	None:intergenic	30.0%
ATGTTTCAACTCAAATCTTC+TGG	-	chr8.2:40998138-40998157	MS.gene25450:intron	30.0%
TAGCAGAGTTAAGTTTAAGT+TGG	+	chr8.2:40998589-40998608	None:intergenic	30.0%
TGGAAACTTTAATAGTATGG+TGG	-	chr8.2:40997802-40997821	MS.gene25450:intron	30.0%
TTCAATGGACATAACAAACA+AGG	-	chr8.2:40998514-40998533	MS.gene25450:CDS	30.0%
ATATGGCAACGTATACAATC+CGG	+	chr8.2:40997346-40997365	None:intergenic	35.0%
ATTCACGTACAAGGATATCA+AGG	-	chr8.2:40998410-40998429	MS.gene25450:CDS	35.0%
CTCTGGATAACTTAAAACAG+GGG	+	chr8.2:40998083-40998102	None:intergenic	35.0%
CTGACATATATTCACGTACA+AGG	-	chr8.2:40998401-40998420	MS.gene25450:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	40997235	40998619	40997235	ID=MS.gene25450
chr8.2	mRNA	40997235	40998619	40997235	ID=MS.gene25450.t1;Parent=MS.gene25450
chr8.2	exon	40998263	40998619	40998263	ID=MS.gene25450.t1.exon1;Parent=MS.gene25450.t1
chr8.2	CDS	40998263	40998619	40998263	ID=cds.MS.gene25450.t1;Parent=MS.gene25450.t1
chr8.2	exon	40997914	40998087	40997914	ID=MS.gene25450.t1.exon2;Parent=MS.gene25450.t1
chr8.2	CDS	40997914	40998087	40997914	ID=cds.MS.gene25450.t1;Parent=MS.gene25450.t1
chr8.2	exon	40997638	40997790	40997638	ID=MS.gene25450.t1.exon3;Parent=MS.gene25450.t1
chr8.2	CDS	40997638	40997790	40997638	ID=cds.MS.gene25450.t1;Parent=MS.gene25450.t1
chr8.2	exon	40997235	40997519	40997235	ID=MS.gene25450.t1.exon4;Parent=MS.gene25450.t1
chr8.2	CDS	40997235	40997519	40997235	ID=cds.MS.gene25450.t1;Parent=MS.gene25450.t1

Gene Sequence

>MS.gene25450

ATGGCAGAGTCAAGCGTGGAACCACGGAAAGAAGAAGAAGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGATGATTTCACAGACAGGATTAGTAGTTTACCGGATTGTATACGTTGCCATATAATGTCCTTCCTCCCGAGTCCGACCATTACCTCTGTGAGGACTATGAGTCTTGTGTCTCGTAGGTGGCGCCACCTCTGGCAGCAGCTACAAGTCTTCTTCTTTATAGACGAGAACGACGACGTAGATTTCAACGACTTTCCATTGTTCGTCAACACTGTGCTCACCCTCCGCAGATTCCCCAACATTCGAAAATTTTGTCTATGGGAAGAACCTCCTACTATGCAGGTACGACAATGTGAATCCAATAGTTTCCTTTCTCTCTGCCTGCCCCATGCTGGAAACTCTGGAAATGCCCTTTTACAATATAGACATATCTTTGACCAAAGTTCCCGTTCCACCTTCCTCCAGCTCCAACAGATTGAAATCCCCCAATTACAATTTCAATTGAACTTATTTTGAGTTAGATATAGATCCCCGTGTCACATTGGGCATCATTGGAAACTTTAATAGTATGGTGGAAGTATTTCTCGATGTCATTTCCTCCGATGAAGACGAATTTGTCGACCCTATTCTCAAAGACATCCGAGATTACAAATATGAAATTTCTCTACTTTCGCGTCATTTGACATCAAAGGTGAAGTTTTTTTTTTTTTTTTGAATGGAAAATGTTAGTAATTAGTTCTTATGGTTTATATGTTTTTTGATGTACGTAGTTGGTACTAATATTGCACCATCGCCTGTTGTTATCGTGTACCAGCAGTGCCCACTTCCTGCCCCTGTTTTAAGTTATCCAGAGTTTCACAAATTAGATCATCTAAAGTTAATTCTTCCATGTTTCAACTCAAATCTTCTGGTCCACATACTTGAGAAATGCCACAAGCTTCGAGTTCTCATCATTCAGAGCAGCAAGGTATGTTGACTTCTCAAGTTTAATTTAATCTATGTCTGGTTTGTCTATATATGCATGCAATCTATCTGTGCATAAATATATTTTTGAGTCATGGCATGGTGTCTCTTGTTGCTGGTAGGAGGAACCACCACCATTGAGAACATGGCAGCCGGAGTCAACAGTTTCTGAATGTCTCAAATCCCACCTGACATATATTCACGTACAAGGATATCAAGGACTTGAAGATGAGTTAACATTTGTTGAATATATTTTGCGGAATGGGCTTGTTTTGGAGACAGTGCTTATTTTCTTTGATATTTCAATGGACATAACAAACAAGGACTGCTCTTTCAAAAGATTATCTGAGATACCAATGGGTTCTGCCACGTGCCAACTTAAACTTAACTCTGCTATAACTCCTTAA

Protein sequence

>MS.gene25450.t1

MAESSVEPRKEEEEEEEEEEEEEEEEDDFTDRISSLPDCIRCHIMSFLPSPTITSVRTMSLVSRRWRHLWQQLQVFFFIDENDDVDFNDFPLFVNTVLTLRRFPNIRKFCLWEEPPTMQLDIDPRVTLGIIGNFNSMVEVFLDVISSDEDEFVDPILKDIRDYKYEISLLSRHLTSKQCPLPAPVLSYPEFHKLDHLKLILPCFNSNLLVHILEKCHKLRVLIIQSSKEEPPPLRTWQPESTVSECLKSHLTYIHVQGYQGLEDELTFVEYILRNGLVLETVLIFFDISMDITNKDCSFKRLSEIPMGSATCQLKLNSAITP