AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25450.t1 MTR_8g070950 72.727 198 50 4 128 322 243 439 1.21e-90 277
MS.gene25450.t1 MTR_8g070950 51.562 128 36 4 1 120 1 110 1.98e-22 97.4
MS.gene25450.t1 MTR_8g062010 66.502 203 66 2 120 321 233 434 4.89e-85 263
MS.gene25450.t1 MTR_8g062010 58.621 87 28 2 31 111 9 93 1.15e-20 92.4
MS.gene25450.t1 MTR_8g469020 62.312 199 72 3 125 322 260 456 9.88e-82 255
MS.gene25450.t1 MTR_8g469020 54.118 85 32 2 30 109 6 88 1.10e-16 80.9
MS.gene25450.t1 MTR_8g062300 61.321 212 66 2 126 321 270 481 8.24e-80 251
MS.gene25450.t1 MTR_8g062300 53.982 113 42 4 1 111 1 105 9.66e-23 98.6
MS.gene25450.t1 MTR_8g062310 63.265 196 70 2 128 322 239 433 4.51e-78 245
MS.gene25450.t1 MTR_8g062310 60.759 79 28 2 31 109 25 100 7.02e-21 92.8
MS.gene25450.t1 MTR_8g058965 63.265 196 70 2 127 321 266 460 1.43e-76 242
MS.gene25450.t1 MTR_8g058965 55.319 94 35 2 21 109 3 94 4.51e-22 96.7
MS.gene25450.t1 MTR_2g086020 45.846 325 110 8 59 322 1 320 1.59e-73 229
MS.gene25450.t1 MTR_8g062080 59.314 204 74 4 126 321 247 449 1.32e-68 221
MS.gene25450.t1 MTR_8g062080 58.333 84 31 2 30 111 22 103 1.01e-19 89.7
MS.gene25450.t1 MTR_4g050250 60.563 142 54 2 125 264 280 421 1.24e-50 173
MS.gene25450.t1 MTR_4g050250 45.370 108 46 3 14 111 4 108 2.77e-18 85.5
MS.gene25450.t1 MTR_8g467790 49.351 154 49 3 120 271 378 504 2.79e-36 137
MS.gene25450.t1 MTR_8g467790 52.885 104 38 2 17 111 134 235 3.22e-16 79.3
MS.gene25450.t1 MTR_8g014260 42.246 187 106 2 131 317 267 451 7.11e-36 135
MS.gene25450.t1 MTR_4g024710 40.107 187 109 3 131 317 164 347 2.06e-30 118
MS.gene25450.t1 MTR_8g062270 37.186 199 105 5 129 316 264 453 3.42e-28 114
MS.gene25450.t1 MTR_8g062270 50.526 95 33 4 28 111 13 104 5.79e-15 75.5
MS.gene25450.t1 MTR_2g083840 39.691 194 110 4 129 317 266 457 8.09e-28 112
MS.gene25450.t1 MTR_2g083840 46.078 102 48 3 12 108 2 101 2.90e-18 85.5
MS.gene25450.t1 MTR_8g077460 46.512 129 67 2 186 314 246 372 1.33e-27 111
MS.gene25450.t1 MTR_8g467800 34.649 228 127 7 110 322 238 458 1.08e-26 109
MS.gene25450.t1 MTR_8g467800 56.842 95 36 3 19 111 8 99 2.24e-22 97.4
MS.gene25450.t1 MTR_8g468810 34.956 226 130 7 105 317 242 463 1.01e-25 107
MS.gene25450.t1 MTR_8g468810 47.664 107 46 3 10 108 2 106 3.27e-19 88.2
MS.gene25450.t1 MTR_8g058970 35.586 222 127 7 110 317 258 477 5.49e-25 105
MS.gene25450.t1 MTR_8g058970 47.115 104 47 3 14 111 2 103 9.19e-20 90.1
MS.gene25450.t1 MTR_3g099800 57.471 87 35 2 230 314 19 105 8.65e-25 96.7
MS.gene25450.t1 MTR_8g014210 43.182 132 73 2 186 317 102 231 2.24e-24 99.4
MS.gene25450.t1 MTR_8g467800 56.842 95 36 3 19 111 8 99 5.87e-23 97.8
MS.gene25450.t1 MTR_8g014160 47.664 107 54 2 209 314 317 422 1.42e-22 97.8
MS.gene25450.t1 MTR_8g468840 35.616 219 123 8 110 317 239 450 2.03e-22 97.4
MS.gene25450.t1 MTR_8g468840 47.000 100 42 3 20 111 3 99 5.92e-14 72.4
MS.gene25450.t1 MTR_4g032730 33.019 212 113 5 131 317 260 467 6.51e-22 96.3
MS.gene25450.t1 MTR_4g032730 36.364 154 73 5 19 156 3 147 7.87e-16 78.2
MS.gene25450.t1 MTR_5g092960 35.354 198 108 6 131 314 268 459 2.29e-21 94.4
MS.gene25450.t1 MTR_5g092960 46.535 101 44 2 19 111 7 105 1.60e-17 83.2
MS.gene25450.t1 MTR_4g024745 40.491 163 94 3 133 295 120 279 2.54e-21 92.0
MS.gene25450.t1 MTR_8g468810 48.980 98 42 2 18 109 12 107 9.84e-20 88.6
MS.gene25450.t1 MTR_8g468810 47.664 107 46 3 10 108 2 106 1.45e-19 88.2
MS.gene25450.t1 MTR_8g468810 49.485 97 41 2 18 108 12 106 1.93e-19 88.2
MS.gene25450.t1 MTR_8g468810 47.222 108 47 3 10 109 2 107 3.28e-19 87.4
MS.gene25450.t1 MTR_8g445780 38.571 140 80 3 133 270 255 390 4.27e-18 84.7
MS.gene25450.t1 MTR_5g092960 46.078 102 45 2 18 111 6 105 2.02e-17 82.8
MS.gene25450.t1 MTR_8g468760 49.485 97 35 3 24 109 2 95 1.75e-16 80.1
MS.gene25450.t1 MTR_8g468760 34.868 152 89 3 173 316 269 418 7.10e-15 75.1
MS.gene25450.t1 MTR_8g014230 43.590 78 43 1 228 305 17 93 9.68e-15 69.7
MS.gene25450.t1 MTR_5g084070 27.586 232 161 5 83 309 190 419 3.35e-14 73.2
MS.gene25450.t1 MTR_5g021550 51.579 95 34 6 24 111 14 103 6.02e-14 68.9
MS.gene25450.t1 MTR_1g041245 35.036 137 80 3 189 317 251 386 8.37e-12 65.9
MS.gene25450.t1 MTR_4g024770 41.250 80 30 2 1 80 8 70 3.08e-11 58.9
MS.gene25450.t1 MTR_3g107100 33.571 140 81 3 189 316 258 397 4.38e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 77 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGGAAACTTTAATAGTA+TGG 0.295484 8.2:-40998033 MS.gene25450:CDS
TTTGCGGAATGGGCTTGTTT+TGG 0.305612 8.2:-40997366 MS.gene25450:CDS
CTCGTAGGTGGCGCCACCTC+TGG 0.349675 8.2:-40998410 MS.gene25450:CDS
CGTGTCACATTGGGCATCAT+TGG 0.351168 8.2:-40998050 MS.gene25450:CDS
CTCGGATGTCTTTGAGAATA+GGG 0.355379 8.2:+40997961 None:intergenic
ATGTTTCAACTCAAATCTTC+TGG 0.358687 8.2:-40997694 MS.gene25450:CDS
AACTCTGGATAACTTAAAAC+AGG 0.359210 8.2:+40997750 None:intergenic
AGACAGGATTAGTAGTTTAC+CGG 0.360565 8.2:-40998508 MS.gene25450:CDS
AGAAGAAGAGGAGGAGGAGG+AGG 0.367681 8.2:-40998565 MS.gene25450:CDS
CTTAAAACAGGGGCAGGAAG+TGG 0.370734 8.2:+40997762 None:intergenic
TCGGGAGGAAGGACATTATA+TGG 0.382451 8.2:+40998472 None:intergenic
AGAAGAAGAAGAGGAGGAGG+AGG 0.403771 8.2:-40998568 MS.gene25450:CDS
TCTCGGATGTCTTTGAGAAT+AGG 0.404973 8.2:+40997960 None:intergenic
AAATTTCATATTTGTAATCT+CGG 0.433276 8.2:+40997943 None:intergenic
AACTCTGCTATAACTCCTTA+AGG 0.444957 8.2:-40997233 None:intergenic
CATTGTCGTACCTGCATAGT+AGG 0.447097 8.2:+40998252 None:intergenic
ATAGATCCCCGTGTCACATT+GGG 0.455209 8.2:-40998059 MS.gene25450:CDS
TAGCAGAGTTAAGTTTAAGT+TGG 0.469875 8.2:+40997246 None:intergenic
ACAGAGGTAATGGTCGGACT+CGG 0.477050 8.2:+40998453 None:intergenic
GAAAGAAGAAGAAGAGGAGG+AGG 0.482532 8.2:-40998571 MS.gene25450:CDS
TGATCTAATTTGTGAAACTC+TGG 0.484029 8.2:+40997735 None:intergenic
TCCTCTTCTTCTTCTTTCCG+TGG 0.493433 8.2:+40998576 None:intergenic
CTTGTACGTGAATATATGTC+AGG 0.499325 8.2:+40997432 None:intergenic
TGTGGCATTTCTCAAGTATG+TGG 0.505882 8.2:+40997670 None:intergenic
CATAGTCCTCACAGAGGTAA+TGG 0.510118 8.2:+40998443 None:intergenic
TCCGGCTGCCATGTTCTCAA+TGG 0.513864 8.2:+40997485 None:intergenic
AGGAGGATGATTTCACAGAC+AGG 0.525769 8.2:-40998524 MS.gene25450:CDS
GGATAACTTAAAACAGGGGC+AGG 0.528203 8.2:+40997756 None:intergenic
CTTGTAGCTGCTGCCAGAGG+TGG 0.531131 8.2:+40998397 None:intergenic
AATGGTCGGACTCGGGAGGA+AGG 0.534339 8.2:+40998461 None:intergenic
TATAGATCCCCGTGTCACAT+TGG 0.538369 8.2:-40998060 MS.gene25450:CDS
GAATGTTGGGGAATCTGCGG+AGG 0.539616 8.2:+40998301 None:intergenic
GACAAATTCGTCTTCATCGG+AGG 0.545225 8.2:+40997986 None:intergenic
AATGATGCCCAATGTGACAC+GGG 0.545808 8.2:+40998052 None:intergenic
TTCGCGTCATTTGACATCAA+AGG 0.548380 8.2:-40997913 MS.gene25450:intron
ATTCACGTACAAGGATATCA+AGG 0.550700 8.2:-40997422 MS.gene25450:CDS
AGCACAGTGTTGACGAACAA+TGG 0.553636 8.2:+40998327 None:intergenic
AGATTATCTGAGATACCAAT+GGG 0.556738 8.2:-40997281 MS.gene25450:CDS
AAGATTATCTGAGATACCAA+TGG 0.557854 8.2:-40997282 MS.gene25450:CDS
AACCACCACCATTGAGAACA+TGG 0.560977 8.2:-40997493 MS.gene25450:CDS
CAATGATGCCCAATGTGACA+CGG 0.564076 8.2:+40998051 None:intergenic
ACCATTGAGAACATGGCAGC+CGG 0.564288 8.2:-40997486 MS.gene25450:CDS
CTATGAGTCTTGTGTCTCGT+AGG 0.564360 8.2:-40998425 MS.gene25450:CDS
GTCGACAAATTCGTCTTCAT+CGG 0.566457 8.2:+40997983 None:intergenic
ACCACGGAAAGAAGAAGAAG+AGG 0.569097 8.2:-40998577 MS.gene25450:CDS
CAGAGGTAATGGTCGGACTC+GGG 0.570050 8.2:+40998454 None:intergenic
GGAAGAACCTCCTACTATGC+AGG 0.571484 8.2:-40998262 MS.gene25450:intron
CTGACATATATTCACGTACA+AGG 0.572025 8.2:-40997431 MS.gene25450:CDS
ACGGAAAGAAGAAGAAGAGG+AGG 0.574256 8.2:-40998574 MS.gene25450:CDS
TTCGAATGTTGGGGAATCTG+CGG 0.575364 8.2:+40998298 None:intergenic
GGCTGCCATGTTCTCAATGG+TGG 0.577603 8.2:+40997488 None:intergenic
TGCCATGTTCTCAATGGTGG+TGG 0.582149 8.2:+40997491 None:intergenic
TTAAAACAGGGGCAGGAAGT+GGG 0.586158 8.2:+40997763 None:intergenic
AGAAGATTTGAGTTGAAACA+TGG 0.586977 8.2:+40997696 None:intergenic
TGGAAACTTTAATAGTATGG+TGG 0.597020 8.2:-40998030 MS.gene25450:CDS
ATATGGCAACGTATACAATC+CGG 0.598567 8.2:+40998489 None:intergenic
TGGCACGTGGCAGAACCCAT+TGG 0.600617 8.2:+40997266 None:intergenic
TGAGTCTTGTGTCTCGTAGG+TGG 0.607225 8.2:-40998422 MS.gene25450:CDS
CAGAGTCAAGCGTGGAACCA+CGG 0.612497 8.2:-40998593 MS.gene25450:CDS
CATTCAGAAACTGTTGACTC+CGG 0.626506 8.2:+40997467 None:intergenic
GTACGTGAATATATGTCAGG+TGG 0.626657 8.2:+40997435 None:intergenic
TACGTGAATATATGTCAGGT+GGG 0.631686 8.2:+40997436 None:intergenic
GTCCTCACAGAGGTAATGGT+CGG 0.636486 8.2:+40998447 None:intergenic
TTCAATGGACATAACAAACA+AGG 0.651693 8.2:-40997318 MS.gene25450:CDS
AGACTTGTAGCTGCTGCCAG+AGG 0.654186 8.2:+40998394 None:intergenic
TGATGAGAACTCGAAGCTTG+TGG 0.658774 8.2:+40997652 None:intergenic
TGTCGTACCTGCATAGTAGG+AGG 0.660063 8.2:+40998255 None:intergenic
AACGATGGCAGAGTCAAGCG+TGG 0.660565 8.2:-40998601 None:intergenic
AGGTAATGGTCGGACTCGGG+AGG 0.661364 8.2:+40998457 None:intergenic
AATGTTGGGGAATCTGCGGA+GGG 0.668481 8.2:+40998302 None:intergenic
ACTCTGGATAACTTAAAACA+GGG 0.673974 8.2:+40997751 None:intergenic
CTCTGGATAACTTAAAACAG+GGG 0.678318 8.2:+40997752 None:intergenic
GTTAAGTTTAAGTTGGCACG+TGG 0.678948 8.2:+40997253 None:intergenic
TCTCATCATTCAGAGCAGCA+AGG 0.683961 8.2:-40997637 MS.gene25450:intron
GTCCGACCATTACCTCTGTG+AGG 0.701738 8.2:-40998449 MS.gene25450:CDS
ATGATGCCCAATGTGACACG+GGG 0.739109 8.2:+40998053 None:intergenic
AAGACTCATAGTCCTCACAG+AGG 0.808089 8.2:+40998437 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTTTTTTTTTTTTGAA+TGG - chr8.2:40997946-40997965 MS.gene25450:CDS 10.0%
!! AAATTTCATATTTGTAATCT+CGG + chr8.2:40997892-40997911 None:intergenic 15.0%
!!! ATAAATATATTTTTGAGTCA+TGG - chr8.2:40998288-40998307 MS.gene25450:CDS 15.0%
!!! TATTTTCTTTGATATTTCAA+TGG - chr8.2:40998499-40998518 MS.gene25450:CDS 15.0%
!! AGATATGTCTATATTGTAAA+AGG + chr8.2:40997663-40997682 None:intergenic 20.0%
!! AGTTCAATTGAAATTGTAAT+TGG + chr8.2:40997738-40997757 None:intergenic 20.0%
!! GATATGTCTATATTGTAAAA+GGG + chr8.2:40997662-40997681 None:intergenic 20.0%
!! GTTCAATTGAAATTGTAATT+GGG + chr8.2:40997737-40997756 None:intergenic 20.0%
!! TTCAATTGAAATTGTAATTG+GGG + chr8.2:40997736-40997755 None:intergenic 20.0%
!!! AGTTTAATTTAATCTATGTC+TGG - chr8.2:40998233-40998252 MS.gene25450:intron 20.0%
!!! ATGTTAGTAATTAGTTCTTA+TGG - chr8.2:40997972-40997991 MS.gene25450:CDS 20.0%
!!! CATTTGTTGAATATATTTTG+CGG - chr8.2:40998450-40998469 MS.gene25450:CDS 20.0%
! CATTGGAAACTTTAATAGTA+TGG - chr8.2:40997799-40997818 MS.gene25450:intron 25.0%
! TCAATTGAAATTGTAATTGG+GGG + chr8.2:40997735-40997754 None:intergenic 25.0%
!! ACATTCGAAAATTTTGTCTA+TGG - chr8.2:40997548-40997567 MS.gene25450:intron 25.0%
!! AGACAAAATTTTCGAATGTT+GGG + chr8.2:40997547-40997566 None:intergenic 25.0%
!! CATTCGAAAATTTTGTCTAT+GGG - chr8.2:40997549-40997568 MS.gene25450:intron 25.0%
!! TAGACAAAATTTTCGAATGT+TGG + chr8.2:40997548-40997567 None:intergenic 25.0%
!! TTGAATATATTTTGCGGAAT+GGG - chr8.2:40998456-40998475 MS.gene25450:CDS 25.0%
AACTCTGGATAACTTAAAAC+AGG + chr8.2:40998085-40998104 None:intergenic 30.0%
ACTCTGGATAACTTAAAACA+GGG + chr8.2:40998084-40998103 None:intergenic 30.0%
ATGTTTCAACTCAAATCTTC+TGG - chr8.2:40998138-40998157 MS.gene25450:intron 30.0%
TAGCAGAGTTAAGTTTAAGT+TGG + chr8.2:40998589-40998608 None:intergenic 30.0%
TGGAAACTTTAATAGTATGG+TGG - chr8.2:40997802-40997821 MS.gene25450:intron 30.0%
TTCAATGGACATAACAAACA+AGG - chr8.2:40998514-40998533 MS.gene25450:CDS 30.0%
! AAGATTATCTGAGATACCAA+TGG - chr8.2:40998550-40998569 MS.gene25450:CDS 30.0%
! AGATTATCTGAGATACCAAT+GGG - chr8.2:40998551-40998570 MS.gene25450:CDS 30.0%
! GACAAAATTTTCGAATGTTG+GGG + chr8.2:40997546-40997565 None:intergenic 30.0%
! GTTGAATATATTTTGCGGAA+TGG - chr8.2:40998455-40998474 MS.gene25450:CDS 30.0%
! TGATCTAATTTGTGAAACTC+TGG + chr8.2:40998100-40998119 None:intergenic 30.0%
!! AGAAGATTTGAGTTGAAACA+TGG + chr8.2:40998139-40998158 None:intergenic 30.0%
!!! TATATTTTTGAGTCATGGCA+TGG - chr8.2:40998293-40998312 MS.gene25450:CDS 30.0%
!!! TGTTTTTTGATGTACGTAGT+TGG - chr8.2:40998001-40998020 MS.gene25450:CDS 30.0%
ATATGGCAACGTATACAATC+CGG + chr8.2:40997346-40997365 None:intergenic 35.0%
ATTCACGTACAAGGATATCA+AGG - chr8.2:40998410-40998429 MS.gene25450:CDS 35.0%
CTCTGGATAACTTAAAACAG+GGG + chr8.2:40998083-40998102 None:intergenic 35.0%
CTGACATATATTCACGTACA+AGG - chr8.2:40998401-40998420 MS.gene25450:CDS 35.0%
CTTGTACGTGAATATATGTC+AGG + chr8.2:40998403-40998422 None:intergenic 35.0%
TACGTGAATATATGTCAGGT+GGG + chr8.2:40998399-40998418 None:intergenic 35.0%
! AGACAGGATTAGTAGTTTAC+CGG - chr8.2:40997324-40997343 MS.gene25450:CDS 35.0%
ATTGGGGGATTTCAATCTGT+TGG + chr8.2:40997720-40997739 None:intergenic 40.0%
ATTTCCAGAGTTTCCAGCAT+GGG + chr8.2:40997638-40997657 None:intergenic 40.0%
GCAGAGAGAAAGGAAACTAT+TGG + chr8.2:40997611-40997630 None:intergenic 40.0%
GTACGTGAATATATGTCAGG+TGG + chr8.2:40998400-40998419 None:intergenic 40.0%
GTCGACAAATTCGTCTTCAT+CGG + chr8.2:40997852-40997871 None:intergenic 40.0%
TGTGGCATTTCTCAAGTATG+TGG + chr8.2:40998165-40998184 None:intergenic 40.0%
TTCGCGTCATTTGACATCAA+AGG - chr8.2:40997919-40997938 MS.gene25450:CDS 40.0%
! CTCGGATGTCTTTGAGAATA+GGG + chr8.2:40997874-40997893 None:intergenic 40.0%
! TCTCGGATGTCTTTGAGAAT+AGG + chr8.2:40997875-40997894 None:intergenic 40.0%
!! CATTCAGAAACTGTTGACTC+CGG + chr8.2:40998368-40998387 None:intergenic 40.0%
!! GTTAAGTTTAAGTTGGCACG+TGG + chr8.2:40998582-40998601 None:intergenic 40.0%
AACCACCACCATTGAGAACA+TGG - chr8.2:40998339-40998358 MS.gene25450:CDS 45.0%
AAGACTCATAGTCCTCACAG+AGG + chr8.2:40997398-40997417 None:intergenic 45.0%
AATGATGCCCAATGTGACAC+GGG + chr8.2:40997783-40997802 None:intergenic 45.0%
ACCACGGAAAGAAGAAGAAG+AGG - chr8.2:40997255-40997274 MS.gene25450:CDS 45.0%
ACGGAAAGAAGAAGAAGAGG+AGG - chr8.2:40997258-40997277 MS.gene25450:CDS 45.0%
AGGAGGATGATTTCACAGAC+AGG - chr8.2:40997308-40997327 MS.gene25450:CDS 45.0%
ATAGATCCCCGTGTCACATT+GGG - chr8.2:40997773-40997792 MS.gene25450:intron 45.0%
CAATGATGCCCAATGTGACA+CGG + chr8.2:40997784-40997803 None:intergenic 45.0%
CATAGTCCTCACAGAGGTAA+TGG + chr8.2:40997392-40997411 None:intergenic 45.0%
CATTGTCGTACCTGCATAGT+AGG + chr8.2:40997583-40997602 None:intergenic 45.0%
CATTTCCAGAGTTTCCAGCA+TGG + chr8.2:40997639-40997658 None:intergenic 45.0%
GAAAGAAGAAGAAGAGGAGG+AGG - chr8.2:40997261-40997280 MS.gene25450:CDS 45.0%
GACAAATTCGTCTTCATCGG+AGG + chr8.2:40997849-40997868 None:intergenic 45.0%
GGATAACTTAAAACAGGGGC+AGG + chr8.2:40998079-40998098 None:intergenic 45.0%
GGATTTCAATCTGTTGGAGC+TGG + chr8.2:40997714-40997733 None:intergenic 45.0%
TACACGATAACAACAGGCGA+TGG + chr8.2:40998040-40998059 None:intergenic 45.0%
TATAGATCCCCGTGTCACAT+TGG - chr8.2:40997772-40997791 MS.gene25450:intron 45.0%
TCCTCTTCTTCTTCTTTCCG+TGG + chr8.2:40997259-40997278 None:intergenic 45.0%
TCGGGAGGAAGGACATTATA+TGG + chr8.2:40997363-40997382 None:intergenic 45.0%
TCTCATCATTCAGAGCAGCA+AGG - chr8.2:40998195-40998214 MS.gene25450:intron 45.0%
TGATGAGAACTCGAAGCTTG+TGG + chr8.2:40998183-40998202 None:intergenic 45.0%
TGCTGGTACACGATAACAAC+AGG + chr8.2:40998046-40998065 None:intergenic 45.0%
TTAAAACAGGGGCAGGAAGT+GGG + chr8.2:40998072-40998091 None:intergenic 45.0%
TTCGAATGTTGGGGAATCTG+CGG + chr8.2:40997537-40997556 None:intergenic 45.0%
TTTCAATCTGTTGGAGCTGG+AGG + chr8.2:40997711-40997730 None:intergenic 45.0%
TTTCCAGAGTTTCCAGCATG+GGG + chr8.2:40997637-40997656 None:intergenic 45.0%
! CTATGAGTCTTGTGTCTCGT+AGG - chr8.2:40997407-40997426 MS.gene25450:CDS 45.0%
!! AGCACAGTGTTGACGAACAA+TGG + chr8.2:40997508-40997527 None:intergenic 45.0%
!! TTTGCGGAATGGGCTTGTTT+TGG - chr8.2:40998466-40998485 MS.gene25450:CDS 45.0%
AATCTGTTGGAGCTGGAGGA+AGG + chr8.2:40997707-40997726 None:intergenic 50.0%
AATGTTGGGGAATCTGCGGA+GGG + chr8.2:40997533-40997552 None:intergenic 50.0%
ACCATTGAGAACATGGCAGC+CGG - chr8.2:40998346-40998365 MS.gene25450:CDS 50.0%
AGAAGAAGAAGAGGAGGAGG+AGG - chr8.2:40997264-40997283 MS.gene25450:CDS 50.0%
ATGATGCCCAATGTGACACG+GGG + chr8.2:40997782-40997801 None:intergenic 50.0%
CGTGTCACATTGGGCATCAT+TGG - chr8.2:40997782-40997801 MS.gene25450:intron 50.0%
CTTAAAACAGGGGCAGGAAG+TGG + chr8.2:40998073-40998092 None:intergenic 50.0%
GGAAGAACCTCCTACTATGC+AGG - chr8.2:40997570-40997589 MS.gene25450:intron 50.0%
TGCCATGTTCTCAATGGTGG+TGG + chr8.2:40998344-40998363 None:intergenic 50.0%
TGGTGTCTCTTGTTGCTGGT+AGG - chr8.2:40998313-40998332 MS.gene25450:CDS 50.0%
TGTCGTACCTGCATAGTAGG+AGG + chr8.2:40997580-40997599 None:intergenic 50.0%
TGTCTCTTGTTGCTGGTAGG+AGG - chr8.2:40998316-40998335 MS.gene25450:CDS 50.0%
! ACAGAGGTAATGGTCGGACT+CGG + chr8.2:40997382-40997401 None:intergenic 50.0%
! GTCCTCACAGAGGTAATGGT+CGG + chr8.2:40997388-40997407 None:intergenic 50.0%
! TGAGTCTTGTGTCTCGTAGG+TGG - chr8.2:40997410-40997429 MS.gene25450:CDS 50.0%
AGAAGAAGAGGAGGAGGAGG+AGG - chr8.2:40997267-40997286 MS.gene25450:CDS 55.0%
AGACTTGTAGCTGCTGCCAG+AGG + chr8.2:40997441-40997460 None:intergenic 55.0%
CAGAGTCAAGCGTGGAACCA+CGG - chr8.2:40997239-40997258 MS.gene25450:CDS 55.0%
GAATGTTGGGGAATCTGCGG+AGG + chr8.2:40997534-40997553 None:intergenic 55.0%
GGAAGGTGGAACGGGAACTT+TGG + chr8.2:40997690-40997709 None:intergenic 55.0%
GGCATGGTGTCTCTTGTTGC+TGG - chr8.2:40998309-40998328 MS.gene25450:CDS 55.0%
GGCTGCCATGTTCTCAATGG+TGG + chr8.2:40998347-40998366 None:intergenic 55.0%
GTCCGACCATTACCTCTGTG+AGG - chr8.2:40997383-40997402 MS.gene25450:CDS 55.0%
TCCGGCTGCCATGTTCTCAA+TGG + chr8.2:40998350-40998369 None:intergenic 55.0%
! CAGAGGTAATGGTCGGACTC+GGG + chr8.2:40997381-40997400 None:intergenic 55.0%
AATGGTCGGACTCGGGAGGA+AGG + chr8.2:40997374-40997393 None:intergenic 60.0%
AGAAGAGGAGGAGGAGGAGG+AGG - chr8.2:40997270-40997289 MS.gene25450:CDS 60.0%
CAGAGTTTCCAGCATGGGGC+AGG + chr8.2:40997633-40997652 None:intergenic 60.0%
CATGGGGCAGGCAGAGAGAA+AGG + chr8.2:40997621-40997640 None:intergenic 60.0%
CTGCCCCATGCTGGAAACTC+TGG - chr8.2:40997631-40997650 MS.gene25450:intron 60.0%
CTGTTGGAGCTGGAGGAAGG+TGG + chr8.2:40997704-40997723 None:intergenic 60.0%
CTTGTAGCTGCTGCCAGAGG+TGG + chr8.2:40997438-40997457 None:intergenic 60.0%
GAGCTGGAGGAAGGTGGAAC+GGG + chr8.2:40997698-40997717 None:intergenic 60.0%
GGAGCTGGAGGAAGGTGGAA+CGG + chr8.2:40997699-40997718 None:intergenic 60.0%
TGGCACGTGGCAGAACCCAT+TGG + chr8.2:40998569-40998588 None:intergenic 60.0%
! AGGTAATGGTCGGACTCGGG+AGG + chr8.2:40997378-40997397 None:intergenic 60.0%
AGAGGAGGAGGAGGAGGAGG+AGG - chr8.2:40997273-40997292 MS.gene25450:CDS 65.0%
TCTCTCTGCCTGCCCCATGC+TGG - chr8.2:40997622-40997641 MS.gene25450:intron 65.0%
CTCGTAGGTGGCGCCACCTC+TGG - chr8.2:40997422-40997441 MS.gene25450:CDS 70.0%
GGAGGAGGAGGAGGAGGAGG+AGG - chr8.2:40997276-40997295 MS.gene25450:CDS 70.0%
! GGGCAGGAAGTGGGCACTGC+TGG + chr8.2:40998063-40998082 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 40997235 40998619 40997235 ID=MS.gene25450
chr8.2 mRNA 40997235 40998619 40997235 ID=MS.gene25450.t1;Parent=MS.gene25450
chr8.2 exon 40998263 40998619 40998263 ID=MS.gene25450.t1.exon1;Parent=MS.gene25450.t1
chr8.2 CDS 40998263 40998619 40998263 ID=cds.MS.gene25450.t1;Parent=MS.gene25450.t1
chr8.2 exon 40997914 40998087 40997914 ID=MS.gene25450.t1.exon2;Parent=MS.gene25450.t1
chr8.2 CDS 40997914 40998087 40997914 ID=cds.MS.gene25450.t1;Parent=MS.gene25450.t1
chr8.2 exon 40997638 40997790 40997638 ID=MS.gene25450.t1.exon3;Parent=MS.gene25450.t1
chr8.2 CDS 40997638 40997790 40997638 ID=cds.MS.gene25450.t1;Parent=MS.gene25450.t1
chr8.2 exon 40997235 40997519 40997235 ID=MS.gene25450.t1.exon4;Parent=MS.gene25450.t1
chr8.2 CDS 40997235 40997519 40997235 ID=cds.MS.gene25450.t1;Parent=MS.gene25450.t1
Gene Sequence

>MS.gene25450

ATGGCAGAGTCAAGCGTGGAACCACGGAAAGAAGAAGAAGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGATGATTTCACAGACAGGATTAGTAGTTTACCGGATTGTATACGTTGCCATATAATGTCCTTCCTCCCGAGTCCGACCATTACCTCTGTGAGGACTATGAGTCTTGTGTCTCGTAGGTGGCGCCACCTCTGGCAGCAGCTACAAGTCTTCTTCTTTATAGACGAGAACGACGACGTAGATTTCAACGACTTTCCATTGTTCGTCAACACTGTGCTCACCCTCCGCAGATTCCCCAACATTCGAAAATTTTGTCTATGGGAAGAACCTCCTACTATGCAGGTACGACAATGTGAATCCAATAGTTTCCTTTCTCTCTGCCTGCCCCATGCTGGAAACTCTGGAAATGCCCTTTTACAATATAGACATATCTTTGACCAAAGTTCCCGTTCCACCTTCCTCCAGCTCCAACAGATTGAAATCCCCCAATTACAATTTCAATTGAACTTATTTTGAGTTAGATATAGATCCCCGTGTCACATTGGGCATCATTGGAAACTTTAATAGTATGGTGGAAGTATTTCTCGATGTCATTTCCTCCGATGAAGACGAATTTGTCGACCCTATTCTCAAAGACATCCGAGATTACAAATATGAAATTTCTCTACTTTCGCGTCATTTGACATCAAAGGTGAAGTTTTTTTTTTTTTTTTGAATGGAAAATGTTAGTAATTAGTTCTTATGGTTTATATGTTTTTTGATGTACGTAGTTGGTACTAATATTGCACCATCGCCTGTTGTTATCGTGTACCAGCAGTGCCCACTTCCTGCCCCTGTTTTAAGTTATCCAGAGTTTCACAAATTAGATCATCTAAAGTTAATTCTTCCATGTTTCAACTCAAATCTTCTGGTCCACATACTTGAGAAATGCCACAAGCTTCGAGTTCTCATCATTCAGAGCAGCAAGGTATGTTGACTTCTCAAGTTTAATTTAATCTATGTCTGGTTTGTCTATATATGCATGCAATCTATCTGTGCATAAATATATTTTTGAGTCATGGCATGGTGTCTCTTGTTGCTGGTAGGAGGAACCACCACCATTGAGAACATGGCAGCCGGAGTCAACAGTTTCTGAATGTCTCAAATCCCACCTGACATATATTCACGTACAAGGATATCAAGGACTTGAAGATGAGTTAACATTTGTTGAATATATTTTGCGGAATGGGCTTGTTTTGGAGACAGTGCTTATTTTCTTTGATATTTCAATGGACATAACAAACAAGGACTGCTCTTTCAAAAGATTATCTGAGATACCAATGGGTTCTGCCACGTGCCAACTTAAACTTAACTCTGCTATAACTCCTTAA
Protein sequence

>MS.gene25450.t1

MAESSVEPRKEEEEEEEEEEEEEEEEDDFTDRISSLPDCIRCHIMSFLPSPTITSVRTMSLVSRRWRHLWQQLQVFFFIDENDDVDFNDFPLFVNTVLTLRRFPNIRKFCLWEEPPTMQLDIDPRVTLGIIGNFNSMVEVFLDVISSDEDEFVDPILKDIRDYKYEISLLSRHLTSKQCPLPAPVLSYPEFHKLDHLKLILPCFNSNLLVHILEKCHKLRVLIIQSSKEEPPPLRTWQPESTVSECLKSHLTYIHVQGYQGLEDELTFVEYILRNGLVLETVLIFFDISMDITNKDCSFKRLSEIPMGSATCQLKLNSAITP