Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055877.t1 | XP_003628587.2 | 56.8 | 447 | 146 | 7 | 4 | 409 | 470 | 910 | 5.00E-127 | 464.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055877.t1 | Q8H1R7 | 24.8 | 435 | 261 | 15 | 24 | 409 | 1 | 418 | 6.4e-14 | 80.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055877.t1 | G7LEM0 | 53.4 | 476 | 146 | 8 | 4 | 409 | 7 | 476 | 1.6e-122 | 449.1 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| No data available in table | ||||
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene002112 | MS.gene055877 | PPI |
| MS.gene003456 | MS.gene055877 | PPI |
| MS.gene004045 | MS.gene055877 | PPI |
| MS.gene005794 | MS.gene055877 | PPI |
| MS.gene005795 | MS.gene055877 | PPI |
| MS.gene006692 | MS.gene055877 | PPI |
| MS.gene006725 | MS.gene055877 | PPI |
| MS.gene006727 | MS.gene055877 | PPI |
| MS.gene006728 | MS.gene055877 | PPI |
| MS.gene006730 | MS.gene055877 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055877.t1 | MTR_8g062300 | 53.527 | 482 | 145 | 10 | 1 | 409 | 1 | 476 | 5.69e-159 | 457 |
| MS.gene055877.t1 | MTR_8g062080 | 51.701 | 441 | 142 | 9 | 23 | 409 | 21 | 444 | 1.48e-134 | 394 |
| MS.gene055877.t1 | MTR_8g070950 | 50.545 | 459 | 151 | 10 | 1 | 409 | 1 | 433 | 4.61e-132 | 387 |
| MS.gene055877.t1 | MTR_8g062010 | 53.349 | 433 | 146 | 12 | 23 | 409 | 7 | 429 | 2.39e-130 | 382 |
| MS.gene055877.t1 | MTR_8g062310 | 50.119 | 421 | 158 | 8 | 24 | 409 | 24 | 427 | 1.22e-128 | 378 |
| MS.gene055877.t1 | MTR_2g086020 | 55.278 | 360 | 114 | 4 | 51 | 409 | 1 | 314 | 6.03e-121 | 354 |
| MS.gene055877.t1 | MTR_8g058965 | 45.957 | 470 | 169 | 13 | 9 | 409 | 2 | 455 | 6.74e-118 | 351 |
| MS.gene055877.t1 | MTR_8g469020 | 45.833 | 456 | 168 | 9 | 23 | 409 | 5 | 450 | 7.98e-118 | 351 |
| MS.gene055877.t1 | MTR_8g467790 | 50.829 | 362 | 126 | 10 | 24 | 351 | 147 | 490 | 3.28e-104 | 318 |
| MS.gene055877.t1 | MTR_4g050250 | 43.578 | 436 | 150 | 13 | 1 | 356 | 1 | 420 | 8.68e-97 | 296 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055877.t1 | AT4G10400 | 23.877 | 423 | 270 | 13 | 24 | 409 | 1 | 408 | 1.35e-15 | 78.6 |
| MS.gene055877.t1 | AT4G10400 | 23.877 | 423 | 270 | 13 | 24 | 409 | 1 | 408 | 1.35e-15 | 78.6 |
| MS.gene055877.t1 | AT4G10400 | 23.877 | 423 | 270 | 13 | 24 | 409 | 1 | 408 | 1.35e-15 | 78.6 |
| MS.gene055877.t1 | AT3G42770 | 30.348 | 201 | 118 | 7 | 27 | 214 | 1 | 192 | 1.17e-13 | 73.2 |
| MS.gene055877.t1 | AT1G78760 | 23.043 | 460 | 268 | 16 | 14 | 407 | 1 | 440 | 6.71e-13 | 70.5 |
| MS.gene055877.t1 | AT2G26860 | 31.220 | 205 | 111 | 10 | 24 | 214 | 1 | 189 | 2.03e-12 | 68.9 |
| MS.gene055877.t1 | AT2G26860 | 31.220 | 205 | 111 | 10 | 24 | 214 | 1 | 189 | 2.03e-12 | 68.9 |
| MS.gene055877.t1 | AT1G69630 | 23.341 | 437 | 256 | 14 | 24 | 396 | 17 | 438 | 4.12e-12 | 68.2 |
| MS.gene055877.t1 | AT5G22700 | 23.096 | 394 | 249 | 12 | 25 | 387 | 43 | 413 | 4.86e-12 | 67.8 |
| MS.gene055877.t1 | AT5G50270 | 24.413 | 426 | 247 | 15 | 24 | 392 | 1 | 408 | 5.00e-12 | 67.8 |
Find 90 sgRNAs with CRISPR-Local
Find 132 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACGAAGGATCTATCAATT+TGG | 0.211457 | 8.3:-42694872 | MS.gene055877:CDS |
| AAATATATGGTCGGGCTCTG+GGG | 0.605682 | 8.3:-42694072 | MS.gene055877:CDS |
| AAATCCTTCTATGTGAATAT+AGG | 0.424387 | 8.3:+42694226 | None:intergenic |
| AACAACCACCATTGAGAACA+TGG | 0.554605 | 8.3:-42694295 | MS.gene055877:CDS |
| AACGAAGGATCTATCAATTT+GGG | 0.387155 | 8.3:-42694871 | MS.gene055877:CDS |
| AAGATTAGCAAATATATGGT+CGG | 0.561234 | 8.3:-42694081 | MS.gene055877:CDS |
| AAGTCAATTCTGTTGAAACG+TGG | 0.669978 | 8.3:-42695692 | MS.gene055877:CDS |
| AATGATGCAACATTTCTGAA+CGG | 0.642150 | 8.3:+42695822 | None:intergenic |
| AATTCAGGATAATTTAGAAC+AGG | 0.394075 | 8.3:+42694567 | None:intergenic |
| ACCATTGAGAACATGGCAGC+CGG | 0.564288 | 8.3:-42694288 | MS.gene055877:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACGAAGGATCTATCAATT+TGG | - | chr8.3:42695215-42695234 | MS.gene055877:intron | 30.0% | |
| AACGAAGGATCTATCAATTT+GGG | - | chr8.3:42695216-42695235 | MS.gene055877:intron | 30.0% | |
| AATAGTGAGTTACATCTCAA+AGG | - | chr8.3:42695342-42695361 | MS.gene055877:intron | 30.0% | |
| AATGATGCAACATTTCTGAA+CGG | + | chr8.3:42694268-42694287 | None:intergenic | 30.0% | |
| ACTGATCATGAAAAGACATT+TGG | + | chr8.3:42696045-42696064 | None:intergenic | 30.0% | |
| AGATTAGCAAATATATGGTC+GGG | - | chr8.3:42696007-42696026 | MS.gene055877:CDS | 30.0% | |
| ATGTTTCAACTCAAATCTTC+TGG | - | chr8.3:42695576-42695595 | MS.gene055877:intron | 30.0% | |
| ATTCACATAGAAGGATTTCA+AGG | - | chr8.3:42695866-42695885 | MS.gene055877:CDS | 30.0% | |
| CGACAAATTTACTTTCAACT+GGG | + | chr8.3:42695293-42695312 | None:intergenic | 30.0% | |
| CTTATGAATGACATCAAACA+TGG | + | chr8.3:42694770-42694789 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 42694037 | 42696072 | 42694037 | ID=MS.gene055877 |
| chr8.3 | mRNA | 42694037 | 42696072 | 42694037 | ID=MS.gene055877.t1;Parent=MS.gene055877 |
| chr8.3 | exon | 42695582 | 42696072 | 42695582 | ID=MS.gene055877.t1.exon1;Parent=MS.gene055877.t1 |
| chr8.3 | CDS | 42695582 | 42696072 | 42695582 | ID=cds.MS.gene055877.t1;Parent=MS.gene055877.t1 |
| chr8.3 | exon | 42694828 | 42695146 | 42694828 | ID=MS.gene055877.t1.exon2;Parent=MS.gene055877.t1 |
| chr8.3 | CDS | 42694828 | 42695146 | 42694828 | ID=cds.MS.gene055877.t1;Parent=MS.gene055877.t1 |
| chr8.3 | exon | 42694455 | 42694604 | 42694455 | ID=MS.gene055877.t1.exon3;Parent=MS.gene055877.t1 |
| chr8.3 | CDS | 42694455 | 42694604 | 42694455 | ID=cds.MS.gene055877.t1;Parent=MS.gene055877.t1 |
| chr8.3 | exon | 42694037 | 42694321 | 42694037 | ID=MS.gene055877.t1.exon4;Parent=MS.gene055877.t1 |
| chr8.3 | CDS | 42694037 | 42694321 | 42694037 | ID=cds.MS.gene055877.t1;Parent=MS.gene055877.t1 |
| Gene Sequence |
>MS.gene055877 ATGGCGGAGATGAAACCAGCTAAAGCAGAAGAAGAAGAATTCGACGACGGAGGAGTCACTAACTTCGCCGTCGACAGGTTAAGTAGTTTACCGGACTCGCTAATATGTCATATAATGTCATTCCTCCCAACTAAAACTTCCGTCGCAACAATGAGCCTTGTCTCTCCTAGGTATCTTAATCTCTGGAAGGATCTTCAGGTCTTCTATTTCAACTTTCACCGATCAATTCCGTTCAGAAATGTTGCATCATTCGTAAACTCTGTTCTCATCCTCCGCAAATCCCGCGACATTCAAAATTTTCATCTCACTTTCAATTGTGATCGATTCAAGCAATTGAATTTTGAAGCCTTTCTATCTCAAGTCAATTCTGTTGAAACGTGGATCCTTGCTTCCATTGGAACTCAACTTCAACAACTCTTTCTCGATGTTCCTTTTCGAATTAACCTCCCGGCTTCCTTCTTTCTCAATGCTACTAATATCGTTTGCCTCAGGTACGTTAACCTATTTTAATTCTATCATCACACACACAGACATACAAGTTATTAGCACTAACGTTAACAAGTTAGTGTTTAAGTTTCTTTTTTTCGTTACAAATTCGAAAGCGTTTATATTAGCTGTTGTATTGGGTTTGATTTGAAACAAAATTGTTCCATTACTTATAATATTGTTGATGGAATATTTATTGTAAAATTAGGTTTGAATTCAAAAACAAGATACTGTGCGTAAATAACCATGTTTGATGTCATTCATAAGCACATTTTAACAAAGTTGGCCGAAAAAAATGAGTAATGGTATAAATATTCTATGATTTAAAAGACCAAATTGTAACAAAAAAAAAAAAAAAAAGACTGCAGGAGACATTTGACCTATAATTTATTTTCTATCCACTTACTTTTTAACTTATCTGACTTTTGAAAACACTGCAGTCTTTACGGTGCCATCGTTATCGAGGTGAAACCTTCTTCTGTTCATTTTCCATCACTGAAGAAGCTGGAAATTGGCGCAGATATTGTGGATGTAAATGAAATAGTTTACTTTCTCTCTGGCTCCCCAAACCTGGAAACTTTGCAGGTTTACTTTTACTTTGTCCCTAAAGACTTTGTGACCAAAGTTTTCGTTCCAACTTTGTCCAGCTCCGAAACAGCCAATGACAATTTCACTTGGTCCTACTTTAATATAAACGAAGGATCTATCAATTTGGGTATTGTTGGCAACTTTCACAGTATGGCGGAAGCGTTTCTTGATGTATTTTACCCAGTTGAAAGTAAATTTGTCGACCCTATTCTCAACCTCATCCGAAATGACAATAGTGAGTTACATCTCAAAGGCCTCAAATATGAAGAAGGTGATATATATCTACTTTCACATCATTCGACATCAAAGGTGAGTTTTATTTTTATAGTTATATGTTTTGTATGTTTAGTTTGGTACTAATATTGCATCATCACTTGTTATCGTGTACGTGCAGTGGCCACCTCATGCCCCTGTTCTAAATTATCCTGAATTTCGTGATTTACATCATCTAAAGTTCGTTCTTCCATGTTTCAACTCAAATCTTCTGGTCCATGTGCTTGAGAAATGCCACATGCTTCAAGTTCTCATAATTAAGAGCAACAAGGTTTGTTGACCGTTTTCAAGTTTAATTCAAACAATCTTTGGTCTATTTATATATACATGCATGCATGTCTCTCTATATCTGTACACACAAATATGTTTTTGAGTCATGACATGATGTCCCTTTTTGCTGGTAGGCGGAACAACCACCATTGAGAACATGGCAGCCGGAGTCAACAACAGTTTCTGAATGTCTCAAATCCCACCTGACCTATATTCACATAGAAGGATTTCAAGGACATGAAGATGAGCTGACATTTGCTGAATATATTAGACGGAACGGACTTGTTTTGAAGACAATGCTTGTTTTTTTTGATATTTCAATGGATAAAACGAACAAGTACCGTTCTGTTGAAAGATTAGCAAATATATGGTCGGGCTCTGGGGCATGCCAAATGTCTTTTCATGATCAGTCTTACTGA |
| Protein sequence |
>MS.gene055877.t1 MAEMKPAKAEEEEFDDGGVTNFAVDRLSSLPDSLICHIMSFLPTKTSVATMSLVSPRYLNLWKDLQVFYFNFHRSIPFRNVASFVNSVLILRKSRDIQNFHLTFNCDRFKQLNFEAFLSQVNSVETWILASIGTQLQQLFLDVPFRINLPASFFLNATNIVCLSLYGAIVIEVKPSSVHFPSLKKLEIGADIVDVNEIVYFLSGSPNLETLQVYFYFVPKDFVTKVFVPTLSSSETANDNFTWSYFNINEGSINLGIVGNFHSMAEAFLDWPPHAPVLNYPEFRDLHHLKFVLPCFNSNLLVHVLEKCHMLQVLIIKSNKAEQPPLRTWQPESTTVSECLKSHLTYIHIEGFQGHEDELTFAEYIRRNGLVLKTMLVFFDISMDKTNKYRSVERLANIWSGSGACQMSFHDQSY |
