AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63213

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63213.t1 MTR_8g058965 56.114 458 166 4 9 431 3 460 2.63e-157 452
MS.gene63213.t1 MTR_8g469020 53.744 454 168 6 18 432 6 456 3.16e-154 444
MS.gene63213.t1 MTR_8g070950 53.348 463 161 11 1 432 1 439 7.54e-142 412
MS.gene63213.t1 MTR_8g062010 53.075 439 171 8 17 431 7 434 3.09e-134 393
MS.gene63213.t1 MTR_8g062310 53.009 432 162 10 19 432 25 433 1.63e-133 391
MS.gene63213.t1 MTR_4g050250 52.732 421 150 8 1 373 1 420 4.12e-131 384
MS.gene63213.t1 MTR_8g062300 47.773 494 182 15 1 431 1 481 8.01e-123 365
MS.gene63213.t1 MTR_8g062080 48.380 463 193 13 1 431 1 449 4.06e-121 360
MS.gene63213.t1 MTR_2g083840 42.576 458 231 9 1 427 1 457 4.02e-90 281
MS.gene63213.t1 MTR_8g062270 39.286 448 228 9 16 426 13 453 2.87e-84 265
MS.gene63213.t1 MTR_8g468810 39.574 470 234 14 1 427 1 463 5.54e-83 262
MS.gene63213.t1 MTR_8g014260 39.301 458 240 10 1 427 1 451 2.92e-80 255
MS.gene63213.t1 MTR_8g058970 38.750 480 238 14 1 427 1 477 4.17e-78 250
MS.gene63213.t1 MTR_8g467800 37.527 469 242 11 2 432 3 458 1.79e-76 245
MS.gene63213.t1 MTR_8g467790 41.842 380 162 13 19 372 148 494 4.76e-73 238
MS.gene63213.t1 MTR_8g468840 38.952 439 232 9 19 425 14 448 3.72e-70 229
MS.gene63213.t1 MTR_5g092960 37.748 453 237 11 14 426 14 461 5.85e-70 228
MS.gene63213.t1 MTR_4g032730 35.897 468 244 11 11 426 3 466 9.73e-69 226
MS.gene63213.t1 MTR_8g468760 36.842 456 208 12 12 426 2 418 3.86e-61 204
MS.gene63213.t1 MTR_2g086020 71.233 146 42 0 287 432 175 320 8.94e-61 200
MS.gene63213.t1 MTR_2g086020 47.059 119 49 3 46 159 3 112 3.26e-19 88.6
MS.gene63213.t1 MTR_8g077460 37.150 428 202 14 12 427 3 375 2.38e-60 201
MS.gene63213.t1 MTR_8g468810 38.624 378 184 13 1 337 1 371 1.92e-56 191
MS.gene63213.t1 MTR_8g445780 36.658 401 211 12 7 380 6 390 2.68e-55 188
MS.gene63213.t1 MTR_8g014160 34.459 444 197 14 19 427 41 425 8.44e-55 188
MS.gene63213.t1 MTR_8g468810 39.344 305 145 10 1 271 1 299 7.04e-48 167
MS.gene63213.t1 MTR_8g468810 39.344 305 145 10 1 271 1 299 9.18e-48 166
MS.gene63213.t1 MTR_8g468810 39.344 305 145 10 1 271 1 299 1.24e-47 167
MS.gene63213.t1 MTR_5g092960 35.695 367 197 10 3 332 2 366 3.44e-44 159
MS.gene63213.t1 MTR_8g014210 41.949 236 124 6 200 427 1 231 6.36e-41 145
MS.gene63213.t1 MTR_4g024710 37.544 285 154 7 157 427 73 347 1.02e-40 148
MS.gene63213.t1 MTR_8g467800 35.647 317 163 8 2 287 3 309 3.30e-36 136
MS.gene63213.t1 MTR_5g021550 45.732 164 82 4 1 159 1 162 3.07e-35 128
MS.gene63213.t1 MTR_1g041245 27.842 431 236 15 18 427 10 386 3.59e-32 126
MS.gene63213.t1 MTR_4g024745 35.878 262 156 7 157 410 27 284 3.24e-29 115
MS.gene63213.t1 MTR_8g014290 44.079 152 74 4 19 160 10 160 5.95e-28 108
MS.gene63213.t1 MTR_7g098510 26.531 441 260 14 1 427 1 391 2.80e-25 107
MS.gene63213.t1 MTR_4g024720 42.222 180 79 7 1 159 1 176 6.19e-25 102
MS.gene63213.t1 MTR_3g099800 51.456 103 49 1 323 424 3 105 6.77e-25 98.6
MS.gene63213.t1 MTR_7g056433 26.047 430 253 15 15 427 8 389 7.61e-23 100
MS.gene63213.t1 MTR_7g098570 25.806 434 263 12 1 422 2 388 1.57e-22 100
MS.gene63213.t1 MTR_7g115270 25.513 439 259 14 11 424 4 399 2.03e-22 99.0
MS.gene63213.t1 MTR_7g033915 27.778 432 214 17 17 426 9 364 1.87e-21 95.5
MS.gene63213.t1 MTR_7g098480 26.818 440 256 14 1 426 1 388 1.14e-20 94.4
MS.gene63213.t1 MTR_7g026500 26.437 435 251 15 13 426 8 394 1.82e-20 93.2
MS.gene63213.t1 MTR_7g056430 26.214 412 230 14 19 419 9 357 9.73e-20 92.8
MS.gene63213.t1 MTR_7g056420 26.214 412 230 14 19 419 9 357 1.08e-19 92.4
MS.gene63213.t1 MTR_5g073420 27.612 402 211 17 17 399 9 349 2.50e-18 86.7
MS.gene63213.t1 MTR_8g014230 48.235 85 42 2 337 421 17 99 3.62e-18 80.1
MS.gene63213.t1 MTR_6g040270 28.571 427 224 19 21 424 10 378 5.64e-18 85.5
MS.gene63213.t1 MTR_3g108560 28.082 438 226 15 12 426 17 388 1.04e-17 84.7
MS.gene63213.t1 MTR_6g022500 24.045 445 247 16 12 427 94 476 2.89e-17 84.0
MS.gene63213.t1 MTR_5g084070 23.884 448 285 14 7 422 3 426 4.77e-17 83.2
MS.gene63213.t1 MTR_1g085120 24.362 431 253 14 19 427 92 471 1.87e-16 81.6
MS.gene63213.t1 MTR_1g085120 24.362 431 253 14 19 427 167 546 2.10e-16 81.6
MS.gene63213.t1 MTR_7g055943 24.661 442 248 18 15 427 36 421 2.84e-15 77.8
MS.gene63213.t1 MTR_4g024770 53.968 63 27 1 1 63 8 68 2.90e-15 70.9
MS.gene63213.t1 MTR_7g034110 35.915 142 70 5 19 153 11 138 7.32e-15 75.9
MS.gene63213.t1 MTR_2g082880 27.404 416 250 18 16 397 7 404 9.85e-15 76.3
MS.gene63213.t1 MTR_2g082800 32.237 152 94 4 5 156 13 155 2.78e-14 74.7
MS.gene63213.t1 MTR_4g092430 33.974 156 88 6 7 159 29 172 3.00e-14 74.7
MS.gene63213.t1 MTR_4g092430 33.974 156 88 6 7 159 29 172 3.13e-14 74.7
MS.gene63213.t1 MTR_2g082850 34.884 129 78 3 6 131 9 134 3.63e-14 73.9
MS.gene63213.t1 MTR_7g032380 34.965 143 81 5 19 159 11 143 4.92e-14 73.6
MS.gene63213.t1 MTR_7g032380 32.090 134 84 3 300 427 224 356 4.11e-12 67.8
MS.gene63213.t1 MTR_2g007500 32.680 153 83 3 8 160 3 135 1.75e-13 71.6
MS.gene63213.t1 MTR_2g007400 24.494 445 253 15 19 430 12 406 1.83e-13 72.0
MS.gene63213.t1 MTR_3g023630 29.082 196 115 7 17 205 6 184 3.41e-13 71.2
MS.gene63213.t1 MTR_4g090940 30.435 161 97 6 1 159 141 288 4.82e-13 71.2
MS.gene63213.t1 MTR_7g034090 30.137 146 90 2 17 159 2 138 6.12e-13 70.5
MS.gene63213.t1 MTR_3g040700 24.297 391 208 14 18 386 42 366 8.30e-13 70.1
MS.gene63213.t1 MTR_7g033940 34.014 147 85 5 4 146 76 214 8.30e-13 67.8
MS.gene63213.t1 MTR_8g014180 53.333 60 26 1 1 60 1 58 1.20e-12 63.2
MS.gene63213.t1 MTR_5g083890 24.631 406 245 13 19 397 6 377 1.58e-12 69.7
MS.gene63213.t1 MTR_3g082890 34.706 170 91 6 2 167 80 233 1.94e-12 69.3
MS.gene63213.t1 MTR_3g107100 30.811 185 101 7 14 193 6 168 2.22e-12 68.9
MS.gene63213.t1 MTR_7g032370 32.847 137 84 3 298 427 217 352 2.37e-12 68.2
MS.gene63213.t1 MTR_4g045740 44.828 87 37 4 7 91 9 86 2.90e-12 66.2
MS.gene63213.t1 MTR_3g036330 38.532 109 59 4 6 111 1 104 3.16e-12 67.8
MS.gene63213.t1 MTR_3g023470 32.461 191 105 9 8 195 4 173 3.24e-12 68.2
MS.gene63213.t1 MTR_3g082890 34.524 168 90 6 4 167 2 153 3.34e-12 68.6
MS.gene63213.t1 MTR_5g008920 31.613 155 88 4 9 159 2 142 4.21e-12 67.8
MS.gene63213.t1 MTR_6g088705 31.088 193 112 8 1 191 1 174 4.79e-12 65.9
MS.gene63213.t1 MTR_3g115430 41.333 75 41 1 353 427 54 125 5.21e-12 63.2
MS.gene63213.t1 MTR_7g034115 34.247 146 79 4 18 159 10 142 5.74e-12 67.4
MS.gene63213.t1 MTR_4g056030 34.459 148 82 5 12 159 5 137 6.05e-12 67.8
MS.gene63213.t1 MTR_7g033990 34.815 135 77 6 19 150 11 137 8.41e-12 67.0
MS.gene63213.t1 MTR_7g056447 31.333 150 86 4 19 165 9 144 8.42e-12 66.2
MS.gene63213.t1 MTR_4g056020 35.135 148 81 6 12 159 5 137 9.42e-12 67.0
MS.gene63213.t1 MTR_8g085430 31.847 157 88 5 18 165 10 156 1.53e-11 65.9
MS.gene63213.t1 MTR_3g107160 33.117 154 86 5 16 162 7 150 1.61e-11 65.9
MS.gene63213.t1 MTR_4g098590 33.562 146 86 4 21 160 1 141 1.63e-11 65.9
MS.gene63213.t1 MTR_7g033945 34.459 148 71 6 17 153 9 141 1.93e-11 63.2
MS.gene63213.t1 MTR_6g088700 41.111 90 41 2 6 91 1 82 1.95e-11 65.9
MS.gene63213.t1 MTR_5g058100 31.333 150 96 3 12 159 2 146 2.28e-11 65.5
MS.gene63213.t1 MTR_2g007450 30.055 183 106 5 6 168 1 181 2.55e-11 65.5
MS.gene63213.t1 MTR_1g094760 31.720 186 105 8 7 191 14 178 2.91e-11 65.9
MS.gene63213.t1 MTR_4g051640 34.831 89 52 1 5 93 16 98 3.01e-11 62.0
MS.gene63213.t1 MTR_7g084080 30.612 196 109 8 6 194 1 176 3.08e-11 64.3
MS.gene63213.t1 MTR_5g008950 31.655 139 83 4 297 427 231 365 3.31e-11 65.1
MS.gene63213.t1 MTR_5g008950 32.432 148 81 4 21 165 1 132 3.89e-11 64.7
MS.gene63213.t1 MTR_1g492880 39.362 94 42 3 12 101 3 85 5.31e-11 59.3
MS.gene63213.t1 MTR_2g099980 29.814 161 103 5 1 159 20 172 5.48e-11 64.7
MS.gene63213.t1 MTR_4g092440 40.909 88 42 2 8 93 21 100 7.53e-11 64.3
MS.gene63213.t1 MTR_1g492880 39.362 94 42 3 12 101 3 85 7.74e-11 59.3
MS.gene63213.t1 MTR_4g092440 39.773 88 43 2 8 93 21 100 7.77e-11 64.3
MS.gene63213.t1 MTR_4g050930 30.102 196 115 8 1 193 11 187 9.09e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63213.t1 AT5G56420 24.439 446 271 13 19 427 6 422 7.09e-20 91.7
MS.gene63213.t1 AT5G56420 24.439 446 271 13 19 427 6 422 7.09e-20 91.7
MS.gene63213.t1 AT5G56420 24.439 446 271 13 19 427 6 422 7.09e-20 91.7
MS.gene63213.t1 AT4G26340 25.561 446 267 13 19 427 2 419 5.23e-18 86.3
MS.gene63213.t1 AT1G69630 32.667 150 94 2 16 165 15 157 5.66e-16 80.1
MS.gene63213.t1 AT1G16930 22.887 485 262 18 12 427 8 449 1.80e-15 78.6
MS.gene63213.t1 AT1G16930 22.887 485 262 18 12 427 8 449 1.80e-15 78.6
MS.gene63213.t1 AT1G16930 22.887 485 262 18 12 427 8 449 1.80e-15 78.6
MS.gene63213.t1 AT4G10400 35.461 141 83 4 19 159 2 134 4.97e-15 77.0
MS.gene63213.t1 AT4G10400 35.461 141 83 4 19 159 2 134 4.97e-15 77.0
MS.gene63213.t1 AT4G10400 35.461 141 83 4 19 159 2 134 4.97e-15 77.0
MS.gene63213.t1 AT4G09920 35.294 136 80 4 24 159 7 134 8.85e-15 75.1
MS.gene63213.t1 AT4G09920 35.294 136 80 4 24 159 7 134 8.85e-15 75.1
MS.gene63213.t1 AT4G09920 35.294 136 80 4 24 159 7 134 8.85e-15 75.1
MS.gene63213.t1 AT4G09920 35.294 136 80 4 24 159 7 134 9.63e-15 75.1
MS.gene63213.t1 AT4G09920 35.294 136 80 4 24 159 7 134 9.63e-15 75.1
MS.gene63213.t1 AT3G59190 34.459 148 81 5 19 159 12 150 1.20e-13 72.8
MS.gene63213.t1 AT3G59190 34.459 148 81 5 19 159 12 150 1.65e-13 72.8
MS.gene63213.t1 AT1G78760 29.747 158 97 4 2 159 5 148 9.03e-13 70.1
MS.gene63213.t1 AT3G49150 34.959 123 71 4 14 133 11 127 5.34e-12 67.8
MS.gene63213.t1 AT3G44060 31.429 140 91 2 21 160 1 135 5.93e-12 67.8
MS.gene63213.t1 AT3G59230 30.822 146 89 3 19 159 12 150 7.42e-12 67.4
MS.gene63213.t1 AT3G59200 31.667 120 76 2 19 135 7 123 7.99e-12 67.0
MS.gene63213.t1 AT5G56440 34.752 141 83 4 19 159 2 133 9.11e-12 67.0
MS.gene63213.t1 AT3G59200 27.528 178 110 4 19 193 7 168 9.35e-12 67.4
MS.gene63213.t1 AT3G03040 31.818 154 90 4 19 164 2 148 1.19e-11 67.0
MS.gene63213.t1 AT4G00160 34.694 147 81 4 17 159 14 149 1.28e-11 66.6
MS.gene63213.t1 AT3G59240 34.014 147 81 5 19 159 8 144 1.51e-11 66.6
MS.gene63213.t1 AT3G03030 31.507 146 88 3 19 159 2 140 1.62e-11 65.9
MS.gene63213.t1 AT3G03030 31.507 146 88 3 19 159 2 140 1.90e-11 66.2
MS.gene63213.t1 AT5G56410 31.293 147 85 4 26 159 10 153 2.24e-11 66.2
MS.gene63213.t1 AT5G38392 32.653 147 87 4 19 159 2 142 4.97e-11 62.8

Find 102 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTCAATTTGAATCTTC+TGG 0.058772 8.4:-41493667 MS.gene63213:CDS
AGGAACATTGGTCAGAAATT+TGG 0.157901 8.4:+41494099 None:intergenic
TGAAGTAGATTGCAAAATTC+TGG 0.220987 8.4:+41493708 None:intergenic
AATTCTGGATTATTTATAAC+AGG 0.256845 8.4:+41493723 None:intergenic
AGATGGGATTCGAGACATTT+AGG 0.261103 8.4:+41493428 None:intergenic
AATGATGCCCAATGTGGTTT+TGG 0.312213 8.4:+41493988 None:intergenic
TGCTTCAATTTGAATCTTCT+GGG 0.329846 8.4:-41493666 MS.gene63213:CDS
GGAACATTGGTCAGAAATTT+GGG 0.333801 8.4:+41494100 None:intergenic
CCACCTCTGGAAGCATCTTC+AGG 0.340202 8.4:-41494868 MS.gene63213:CDS
TGTGCCACCTTCCTCCAAAA+AGG 0.351535 8.4:-41494077 MS.gene63213:CDS
CTCGCAGATGGCGCCACCTC+TGG 0.354975 8.4:-41494881 MS.gene63213:CDS
TCAGACATTGCCTTTCTCTC+TGG 0.356495 8.4:-41494159 MS.gene63213:CDS
CCGTAGATGTAATGCATTAT+CGG 0.360156 8.4:+41493871 None:intergenic
TAGGAAGAACCATCACCTTT+AGG 0.373743 8.4:-41493479 MS.gene63213:CDS
GAAACTCGACATGCGCGTTA+TGG 0.374368 8.4:-41494184 MS.gene63213:CDS
TTTCAATTGGGAGGAATGAT+AGG 0.379509 8.4:+41494928 None:intergenic
GTATCCTTCTATGTGAATAT+AGG 0.383394 8.4:+41493403 None:intergenic
AAATAAAATCCCATCGTTTG+AGG 0.383750 8.4:-41494015 MS.gene63213:CDS
GTAGCCACGGCGGTTTCAAT+TGG 0.384370 8.4:+41494915 None:intergenic
AAAACCACATTGGGCATCAT+TGG 0.392776 8.4:-41493986 MS.gene63213:CDS
CTCACCTATATTCACATAGA+AGG 0.400301 8.4:-41493407 MS.gene63213:CDS
ATCAGCATTTGGTCACCATC+GGG 0.411925 8.4:+41494612 None:intergenic
AATATGCAAGTTTCGAGAAC+GGG 0.417149 8.4:+41494132 None:intergenic
TGGCACATGACAGAGCCTTT+TGG 0.421479 8.4:+41493242 None:intergenic
GATGCAGATGTGTCTCCTTA+AGG 0.433765 8.4:-41493209 None:intergenic
AACGGGGCAGCCAGAGAGAA+AGG 0.437002 8.4:+41494149 None:intergenic
TGGGCTCCACCTTCAAGAAC+TGG 0.439888 8.4:-41494652 MS.gene63213:CDS
CTCTGAGGCAGTTCAGAATA+GGG 0.440335 8.4:+41493897 None:intergenic
CCTGTCTTGTTTCTCGCAGA+TGG 0.442850 8.4:-41494893 MS.gene63213:CDS
AGGTCTGCCAAAACCACATT+GGG 0.446150 8.4:-41493995 MS.gene63213:CDS
GCACCAACCTCGTTTCCCTT+AGG 0.448633 8.4:-41494566 MS.gene63213:intron
TCTCTGAGGCAGTTCAGAAT+AGG 0.453518 8.4:+41493896 None:intergenic
CCGTCTCCACTCTGGCATAT+CGG 0.454386 8.4:-41494727 MS.gene63213:CDS
AAGAAGGGAGGATCAGCATT+TGG 0.464635 8.4:+41494601 None:intergenic
ATGTGGATCACTGCCGCCAT+TGG 0.467542 8.4:-41494672 MS.gene63213:CDS
TCAGCTCTTCTTCAAGTCCT+TGG 0.472411 8.4:+41493381 None:intergenic
GTGCAATTCAAGAGGGAAGA+AGG 0.476286 8.4:+41494585 None:intergenic
TAGCCACGGCGGTTTCAATT+GGG 0.476331 8.4:+41494916 None:intergenic
TTCGAATGTCACGGGATGTA+CGG 0.483799 8.4:+41494754 None:intergenic
TGTGGATCACTGCCGCCATT+GGG 0.483997 8.4:-41494671 MS.gene63213:CDS
CAGAAGATTCAAATTGAAGC+AGG 0.487670 8.4:+41493668 None:intergenic
TCAACAATGGAAATTTCTCA+TGG 0.492414 8.4:-41494048 MS.gene63213:CDS
CGTAGATGTAATGCATTATC+GGG 0.495397 8.4:+41493872 None:intergenic
AGATTATCCCGTGCACCAAA+AGG 0.500478 8.4:-41493257 MS.gene63213:CDS
CGAAAGTTCCGTCTCCACTC+TGG 0.511900 8.4:-41494735 MS.gene63213:CDS
CATTTGGTCACCATCGGGGT+TGG 0.512406 8.4:+41494617 None:intergenic
TGTCGAGATCCAGTTCTTGA+AGG 0.515111 8.4:+41494643 None:intergenic
CTATGTGAATATAGGTGAGA+TGG 0.515863 8.4:+41493411 None:intergenic
CCGATAATGCATTACATCTA+CGG 0.525655 8.4:-41493871 MS.gene63213:CDS
AGTTTCAGCCACTTGAGTGA+TGG 0.526074 8.4:+41494201 None:intergenic
GATGTAGCAGTGCGAGTCAT+CGG 0.528849 8.4:+41494833 None:intergenic
TGCAGCTCACATGCTCCTAA+AGG 0.530041 8.4:+41493464 None:intergenic
ATGTGGCACACTAGAGAGTC+CGG 0.535850 8.4:+41494951 None:intergenic
TTTGGCAGACCTCAAACGAT+GGG 0.549037 8.4:+41494006 None:intergenic
TCTCATAATTCAAATAAGCA+AGG 0.549803 8.4:-41493610 MS.gene63213:intron
GGAAGGTGGCACAGGAACAT+TGG 0.549960 8.4:+41494087 None:intergenic
GATCAGCATTTGGTCACCAT+CGG 0.551411 8.4:+41494611 None:intergenic
CCGATATGCCAGAGTGGAGA+CGG 0.554409 8.4:+41494727 None:intergenic
GAGGTCTGCCAAAACCACAT+TGG 0.557086 8.4:-41493996 MS.gene63213:CDS
TTCTGGATTATTTATAACAG+GGG 0.560129 8.4:+41493725 None:intergenic
TATGTGAATATAGGTGAGAT+GGG 0.565909 8.4:+41493412 None:intergenic
CGACTTGATGAGTATTCTAC+CGG 0.568711 8.4:-41494970 MS.gene63213:CDS
CCTGAAGATGCTTCCAGAGG+TGG 0.571117 8.4:+41494868 None:intergenic
TTGGGAGGAATGATAGGATG+TGG 0.576739 8.4:+41494934 None:intergenic
CATCTGCATCAAATTCAAGT+TGG 0.579696 8.4:+41493222 None:intergenic
TCACATGCTCCTAAAGGTGA+TGG 0.580001 8.4:+41493470 None:intergenic
AGTGCGAGTCATCGGAGAAG+TGG 0.583425 8.4:+41494841 None:intergenic
CGAGATCCAGTTCTTGAAGG+TGG 0.585480 8.4:+41494646 None:intergenic
TGACCTAAGGGAAACGAGGT+TGG 0.590159 8.4:+41494563 None:intergenic
TGCAATTCAAGAGGGAAGAA+GGG 0.594414 8.4:+41494586 None:intergenic
GACGGAACTTTCGAATGTCA+CGG 0.599917 8.4:+41494745 None:intergenic
ACGGAACTTTCGAATGTCAC+GGG 0.601922 8.4:+41494746 None:intergenic
AAATATGCAAGTTTCGAGAA+CGG 0.602701 8.4:+41494131 None:intergenic
ATTCTGGATTATTTATAACA+GGG 0.605085 8.4:+41493724 None:intergenic
CACATGTTGATGCAGTTGCT+AGG 0.605641 8.4:+41494690 None:intergenic
CGAGGTTGGTGCAATTCAAG+AGG 0.606867 8.4:+41494577 None:intergenic
GAATGTCACGGGATGTACGG+AGG 0.618589 8.4:+41494757 None:intergenic
CCACGGCGGTTTCAATTGGG+AGG 0.619307 8.4:+41494919 None:intergenic
AATGTCACGGGATGTACGGA+GGG 0.633611 8.4:+41494758 None:intergenic
TTGAAGGTGGAGCCCAATGG+CGG 0.634148 8.4:+41494659 None:intergenic
CTAGCAACTGCATCAACATG+TGG 0.635235 8.4:-41494689 MS.gene63213:CDS
TTTATAACAGGGGCACGAAG+TGG 0.635619 8.4:+41493735 None:intergenic
CCATCTGCGAGAAACAAGAC+AGG 0.637626 8.4:+41494893 None:intergenic
CTCGACATAGCCAACCCCGA+TGG 0.641689 8.4:-41494627 MS.gene63213:CDS
AGAAGATTCAAATTGAAGCA+GGG 0.643008 8.4:+41493669 None:intergenic
TGGCAACGTGCATAGTGTTG+TGG 0.644841 8.4:-41493966 MS.gene63213:CDS
CGGTAGAATACTCATCAAGT+CGG 0.650262 8.4:+41494971 None:intergenic
TTCTTGAAGGTGGAGCCCAA+TGG 0.650619 8.4:+41494656 None:intergenic
ATATGCAAGTTTCGAGAACG+GGG 0.657180 8.4:+41494133 None:intergenic
AGAAACAAGACAGGTAGCCA+CGG 0.675062 8.4:+41494902 None:intergenic
TCAGCATTTGGTCACCATCG+GGG 0.679792 8.4:+41494613 None:intergenic
ATTCACATAGAAGGATACCA+AGG 0.680294 8.4:-41493398 MS.gene63213:CDS
GAGGTTGGTGCAATTCAAGA+GGG 0.693328 8.4:+41494578 None:intergenic
AGACCTGAAGATGCTTCCAG+AGG 0.695151 8.4:+41494865 None:intergenic
AATTCAAGAGGGAAGAAGGG+AGG 0.701014 8.4:+41494589 None:intergenic
AAGCTGACCTAAGGGAAACG+AGG 0.701093 8.4:+41494559 None:intergenic
ATGCATTATCGGGATCTCTG+AGG 0.702931 8.4:+41493882 None:intergenic
CCTCCCAATTGAAACCGCCG+TGG 0.709533 8.4:-41494919 MS.gene63213:CDS
GTTGCCAATGATGCCCAATG+TGG 0.714684 8.4:+41493982 None:intergenic
AACAAGACAGGTAGCCACGG+CGG 0.716839 8.4:+41494905 None:intergenic
AGAGAAGAAGAAGAACACGA+TGG 0.736796 8.4:-41494996 MS.gene63213:CDS
GTTCATCCGATATGCCAGAG+TGG 0.744615 8.4:+41494721 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATAATGAAAGTTAATTTT+GGG + chr8.4:41493894-41493913 None:intergenic 10.0%
!!! TATATAATGAAAGTTAATTT+TGG + chr8.4:41493895-41493914 None:intergenic 10.0%
!! AACTTTCATTATATAATATC+TGG - chr8.4:41493900-41493919 MS.gene63213:CDS 15.0%
!! AATTCTGGATTATTTATAAC+AGG + chr8.4:41494514-41494533 None:intergenic 20.0%
!! AGTTTAATTCAATGAATGTT+TGG - chr8.4:41494662-41494681 MS.gene63213:CDS 20.0%
!! ATTCTGGATTATTTATAACA+GGG + chr8.4:41494513-41494532 None:intergenic 20.0%
!!! TATTTTTGTTGATATTTCGA+TGG - chr8.4:41494925-41494944 MS.gene63213:CDS 20.0%
!!! TGTATCTGTATAACTATTTT+TGG - chr8.4:41494433-41494452 MS.gene63213:intron 20.0%
! AAAAAGGTGAAATTCAACAA+TGG - chr8.4:41494173-41494192 MS.gene63213:CDS 25.0%
! AAAAATATACATGCGATTCA+TGG - chr8.4:41494716-41494735 MS.gene63213:CDS 25.0%
! AGAATGAAATGAAAACAGAA+GGG - chr8.4:41493858-41493877 MS.gene63213:CDS 25.0%
! TAGAATGAAATGAAAACAGA+AGG - chr8.4:41493857-41493876 MS.gene63213:CDS 25.0%
! TCTCATAATTCAAATAAGCA+AGG - chr8.4:41494624-41494643 MS.gene63213:CDS 25.0%
! TTCTGGATTATTTATAACAG+GGG + chr8.4:41494512-41494531 None:intergenic 25.0%
!! ATTGTTGAATTTCACCTTTT+TGG + chr8.4:41494174-41494193 None:intergenic 25.0%
!!! AACGATGGGATTTTATTTTA+AGG + chr8.4:41494217-41494236 None:intergenic 25.0%
AAATAAAATCCCATCGTTTG+AGG - chr8.4:41494219-41494238 MS.gene63213:intron 30.0%
AAATATGCAAGTTTCGAGAA+CGG + chr8.4:41494106-41494125 None:intergenic 30.0%
AGAAGATTCAAATTGAAGCA+GGG + chr8.4:41494568-41494587 None:intergenic 30.0%
GTAGATAATGCTAACTTTCA+TGG - chr8.4:41493942-41493961 MS.gene63213:CDS 30.0%
GTATCCTTCTATGTGAATAT+AGG + chr8.4:41494834-41494853 None:intergenic 30.0%
TATGTGAATATAGGTGAGAT+GGG + chr8.4:41494825-41494844 None:intergenic 30.0%
TCAACAATGGAAATTTCTCA+TGG - chr8.4:41494186-41494205 MS.gene63213:CDS 30.0%
TGAAGTAGATTGCAAAATTC+TGG + chr8.4:41494529-41494548 None:intergenic 30.0%
TGCTTCAATTTGAATCTTCT+GGG - chr8.4:41494568-41494587 MS.gene63213:CDS 30.0%
TTGTACATGATAACAAGTGA+TGG + chr8.4:41494472-41494491 None:intergenic 30.0%
! CATATCTTGATGTCTTTTGT+CGG - chr8.4:41494294-41494313 MS.gene63213:intron 30.0%
! GCTGAATATATTTTGCAGAA+CGG - chr8.4:41494881-41494900 MS.gene63213:CDS 30.0%
AATATGCAAGTTTCGAGAAC+GGG + chr8.4:41494105-41494124 None:intergenic 35.0%
AGGAACATTGGTCAGAAATT+TGG + chr8.4:41494138-41494157 None:intergenic 35.0%
ATTCACATAGAAGGATACCA+AGG - chr8.4:41494836-41494855 MS.gene63213:CDS 35.0%
CAGAAGATTCAAATTGAAGC+AGG + chr8.4:41494569-41494588 None:intergenic 35.0%
CATCTGCATCAAATTCAAGT+TGG + chr8.4:41495015-41495034 None:intergenic 35.0%
CCGATAATGCATTACATCTA+CGG - chr8.4:41494363-41494382 MS.gene63213:intron 35.0%
CCGTAGATGTAATGCATTAT+CGG + chr8.4:41494366-41494385 None:intergenic 35.0%
CGTAGATGTAATGCATTATC+GGG + chr8.4:41494365-41494384 None:intergenic 35.0%
CTATGTGAATATAGGTGAGA+TGG + chr8.4:41494826-41494845 None:intergenic 35.0%
CTCACCTATATTCACATAGA+AGG - chr8.4:41494827-41494846 MS.gene63213:CDS 35.0%
CTGCTTCAATTTGAATCTTC+TGG - chr8.4:41494567-41494586 MS.gene63213:CDS 35.0%
GGAACATTGGTCAGAAATTT+GGG + chr8.4:41494137-41494156 None:intergenic 35.0%
TAACTGAAAAGCTGACCTAA+GGG + chr8.4:41493686-41493705 None:intergenic 35.0%
TTGTTATCATGTACAAGCAG+TGG - chr8.4:41494476-41494495 MS.gene63213:intron 35.0%
TTTCAATTGGGAGGAATGAT+AGG + chr8.4:41493309-41493328 None:intergenic 35.0%
! GTTGAATTTCACCTTTTTGG+AGG + chr8.4:41494171-41494190 None:intergenic 35.0%
! TTCATTTTCCATCACTCAAG+TGG - chr8.4:41494025-41494044 MS.gene63213:CDS 35.0%
!! AATTTCACCTTTTTGGAGGA+AGG + chr8.4:41494167-41494186 None:intergenic 35.0%
!! GGGATAATCTTTTGAGAGAA+CGG + chr8.4:41494967-41494986 None:intergenic 35.0%
AAAACCACATTGGGCATCAT+TGG - chr8.4:41494248-41494267 MS.gene63213:intron 40.0%
AGAGAAGAAGAAGAACACGA+TGG - chr8.4:41493238-41493257 MS.gene63213:CDS 40.0%
AGATGGGATTCGAGACATTT+AGG + chr8.4:41494809-41494828 None:intergenic 40.0%
ATATGCAAGTTTCGAGAACG+GGG + chr8.4:41494104-41494123 None:intergenic 40.0%
CGACTTGATGAGTATTCTAC+CGG - chr8.4:41493264-41493283 MS.gene63213:CDS 40.0%
CGGTAGAATACTCATCAAGT+CGG + chr8.4:41493266-41493285 None:intergenic 40.0%
GTAACTGAAAAGCTGACCTA+AGG + chr8.4:41493687-41493706 None:intergenic 40.0%
GTTCTTCCTATCGACAACAA+GGG + chr8.4:41494745-41494764 None:intergenic 40.0%
TAGGAAGAACCATCACCTTT+AGG - chr8.4:41494755-41494774 MS.gene63213:CDS 40.0%
TGCAATTCAAGAGGGAAGAA+GGG + chr8.4:41493651-41493670 None:intergenic 40.0%
! AATGATGCCCAATGTGGTTT+TGG + chr8.4:41494249-41494268 None:intergenic 40.0%
AAGAAGGGAGGATCAGCATT+TGG + chr8.4:41493636-41493655 None:intergenic 45.0%
AATTCAAGAGGGAAGAAGGG+AGG + chr8.4:41493648-41493667 None:intergenic 45.0%
ACGGAACTTTCGAATGTCAC+GGG + chr8.4:41493491-41493510 None:intergenic 45.0%
AGAAACAAGACAGGTAGCCA+CGG + chr8.4:41493335-41493354 None:intergenic 45.0%
AGATTATCCCGTGCACCAAA+AGG - chr8.4:41494977-41494996 MS.gene63213:CDS 45.0%
AGGTCTGCCAAAACCACATT+GGG - chr8.4:41494239-41494258 MS.gene63213:intron 45.0%
AGTTTCAGCCACTTGAGTGA+TGG + chr8.4:41494036-41494055 None:intergenic 45.0%
ATCAGCATTTGGTCACCATC+GGG + chr8.4:41493625-41493644 None:intergenic 45.0%
ATGCATTATCGGGATCTCTG+AGG + chr8.4:41494355-41494374 None:intergenic 45.0%
CACATGTTGATGCAGTTGCT+AGG + chr8.4:41493547-41493566 None:intergenic 45.0%
CTAGCAACTGCATCAACATG+TGG - chr8.4:41493545-41493564 MS.gene63213:intron 45.0%
GACGGAACTTTCGAATGTCA+CGG + chr8.4:41493492-41493511 None:intergenic 45.0%
GATCAGCATTTGGTCACCAT+CGG + chr8.4:41493626-41493645 None:intergenic 45.0%
GCTGCGTCATTCGACAAAAA+AGG - chr8.4:41494385-41494404 MS.gene63213:intron 45.0%
GGTTCTTCCTATCGACAACA+AGG + chr8.4:41494746-41494765 None:intergenic 45.0%
GTGCAATTCAAGAGGGAAGA+AGG + chr8.4:41493652-41493671 None:intergenic 45.0%
TCACATGCTCCTAAAGGTGA+TGG + chr8.4:41494767-41494786 None:intergenic 45.0%
TCAGACATTGCCTTTCTCTC+TGG - chr8.4:41494075-41494094 MS.gene63213:CDS 45.0%
TCAGCTCTTCTTCAAGTCCT+TGG + chr8.4:41494856-41494875 None:intergenic 45.0%
TGTCGAGATCCAGTTCTTGA+AGG + chr8.4:41493594-41493613 None:intergenic 45.0%
TTCGAATGTCACGGGATGTA+CGG + chr8.4:41493483-41493502 None:intergenic 45.0%
TTGGGAGGAATGATAGGATG+TGG + chr8.4:41493303-41493322 None:intergenic 45.0%
TTTGGCAGACCTCAAACGAT+GGG + chr8.4:41494231-41494250 None:intergenic 45.0%
! TCTAAACGCTGCAGTCTTGT+TGG - chr8.4:41493970-41493989 MS.gene63213:CDS 45.0%
! TTTATAACAGGGGCACGAAG+TGG + chr8.4:41494502-41494521 None:intergenic 45.0%
! TTTTGGCAGACCTCAAACGA+TGG + chr8.4:41494232-41494251 None:intergenic 45.0%
!! CTCTGAGGCAGTTCAGAATA+GGG + chr8.4:41494340-41494359 None:intergenic 45.0%
!! GAGGTTGGTGCAATTCAAGA+GGG + chr8.4:41493659-41493678 None:intergenic 45.0%
!! TCTCTGAGGCAGTTCAGAAT+AGG + chr8.4:41494341-41494360 None:intergenic 45.0%
!! TTCACCTTTTTGGAGGAAGG+TGG + chr8.4:41494164-41494183 None:intergenic 45.0%
AAGCTGACCTAAGGGAAACG+AGG + chr8.4:41493678-41493697 None:intergenic 50.0%
AATGTCACGGGATGTACGGA+GGG + chr8.4:41493479-41493498 None:intergenic 50.0%
AGACCTGAAGATGCTTCCAG+AGG + chr8.4:41493372-41493391 None:intergenic 50.0%
ATGTGGCACACTAGAGAGTC+CGG + chr8.4:41493286-41493305 None:intergenic 50.0%
CCATCTGCGAGAAACAAGAC+AGG + chr8.4:41493344-41493363 None:intergenic 50.0%
CGAGATCCAGTTCTTGAAGG+TGG + chr8.4:41493591-41493610 None:intergenic 50.0%
GAAACTCGACATGCGCGTTA+TGG - chr8.4:41494050-41494069 MS.gene63213:CDS 50.0%
GAGGTCTGCCAAAACCACAT+TGG - chr8.4:41494238-41494257 MS.gene63213:intron 50.0%
GATGTAGCAGTGCGAGTCAT+CGG + chr8.4:41493404-41493423 None:intergenic 50.0%
GTTCATCCGATATGCCAGAG+TGG + chr8.4:41493516-41493535 None:intergenic 50.0%
GTTGCCAATGATGCCCAATG+TGG + chr8.4:41494255-41494274 None:intergenic 50.0%
TAGCCACGGCGGTTTCAATT+GGG + chr8.4:41493321-41493340 None:intergenic 50.0%
TCAGCATTTGGTCACCATCG+GGG + chr8.4:41493624-41493643 None:intergenic 50.0%
TGACCTAAGGGAAACGAGGT+TGG + chr8.4:41493674-41493693 None:intergenic 50.0%
TGCAGCTCACATGCTCCTAA+AGG + chr8.4:41494773-41494792 None:intergenic 50.0%
TGGCAACGTGCATAGTGTTG+TGG - chr8.4:41494268-41494287 MS.gene63213:intron 50.0%
TGTGCCACCTTCCTCCAAAA+AGG - chr8.4:41494157-41494176 MS.gene63213:CDS 50.0%
TTCTTGAAGGTGGAGCCCAA+TGG + chr8.4:41493581-41493600 None:intergenic 50.0%
! TGGCACATGACAGAGCCTTT+TGG + chr8.4:41494995-41495014 None:intergenic 50.0%
! TGGTGTCCCTTGTTGTCGAT+AGG - chr8.4:41494736-41494755 MS.gene63213:CDS 50.0%
!! CCTGTCTTGTTTCTCGCAGA+TGG - chr8.4:41493341-41493360 MS.gene63213:CDS 50.0%
!! CGAGGTTGGTGCAATTCAAG+AGG + chr8.4:41493660-41493679 None:intergenic 50.0%
!! TGACAGAGCCTTTTGGTGCA+CGG + chr8.4:41494988-41495007 None:intergenic 50.0%
!! TTTTTGGAGGAAGGTGGCAC+AGG + chr8.4:41494158-41494177 None:intergenic 50.0%
AACAAGACAGGTAGCCACGG+CGG + chr8.4:41493332-41493351 None:intergenic 55.0%
AGTGCGAGTCATCGGAGAAG+TGG + chr8.4:41493396-41493415 None:intergenic 55.0%
ATGTGGATCACTGCCGCCAT+TGG - chr8.4:41493562-41493581 MS.gene63213:intron 55.0%
CATTTGGTCACCATCGGGGT+TGG + chr8.4:41493620-41493639 None:intergenic 55.0%
CCGATATGCCAGAGTGGAGA+CGG + chr8.4:41493510-41493529 None:intergenic 55.0%
CCGTCTCCACTCTGGCATAT+CGG - chr8.4:41493507-41493526 MS.gene63213:intron 55.0%
CCTGAAGATGCTTCCAGAGG+TGG + chr8.4:41493369-41493388 None:intergenic 55.0%
CGAAAGTTCCGTCTCCACTC+TGG - chr8.4:41493499-41493518 MS.gene63213:intron 55.0%
GAATGTCACGGGATGTACGG+AGG + chr8.4:41493480-41493499 None:intergenic 55.0%
GCACCAACCTCGTTTCCCTT+AGG - chr8.4:41493668-41493687 MS.gene63213:CDS 55.0%
GGAAGGTGGCACAGGAACAT+TGG + chr8.4:41494150-41494169 None:intergenic 55.0%
GTAGCCACGGCGGTTTCAAT+TGG + chr8.4:41493322-41493341 None:intergenic 55.0%
TGGGCTCCACCTTCAAGAAC+TGG - chr8.4:41493582-41493601 MS.gene63213:intron 55.0%
TGTGGATCACTGCCGCCATT+GGG - chr8.4:41493563-41493582 MS.gene63213:intron 55.0%
TTGAAGGTGGAGCCCAATGG+CGG + chr8.4:41493578-41493597 None:intergenic 55.0%
!! CCACCTCTGGAAGCATCTTC+AGG - chr8.4:41493366-41493385 MS.gene63213:CDS 55.0%
!! GACAGAGCCTTTTGGTGCAC+GGG + chr8.4:41494987-41495006 None:intergenic 55.0%
AACGGGGCAGCCAGAGAGAA+AGG + chr8.4:41494088-41494107 None:intergenic 60.0%
CCACGGCGGTTTCAATTGGG+AGG + chr8.4:41493318-41493337 None:intergenic 60.0%
CCTCCCAATTGAAACCGCCG+TGG - chr8.4:41493315-41493334 MS.gene63213:CDS 60.0%
CTCGACATAGCCAACCCCGA+TGG - chr8.4:41493607-41493626 MS.gene63213:intron 60.0%
CTCGCAGATGGCGCCACCTC+TGG - chr8.4:41493353-41493372 MS.gene63213:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 41493211 41495045 41493211 ID=MS.gene63213
chr8.4 mRNA 41493211 41495045 41493211 ID=MS.gene63213.t1;Parent=MS.gene63213
chr8.4 exon 41494567 41495045 41494567 ID=MS.gene63213.t1.exon1;Parent=MS.gene63213.t1
chr8.4 CDS 41494567 41495045 41494567 ID=cds.MS.gene63213.t1;Parent=MS.gene63213.t1
chr8.4 exon 41493850 41494231 41493850 ID=MS.gene63213.t1.exon2;Parent=MS.gene63213.t1
chr8.4 CDS 41493850 41494231 41493850 ID=cds.MS.gene63213.t1;Parent=MS.gene63213.t1
chr8.4 exon 41493611 41493760 41493611 ID=MS.gene63213.t1.exon3;Parent=MS.gene63213.t1
chr8.4 CDS 41493611 41493760 41493611 ID=cds.MS.gene63213.t1;Parent=MS.gene63213.t1
chr8.4 exon 41493211 41493498 41493211 ID=MS.gene63213.t1.exon4;Parent=MS.gene63213.t1
chr8.4 CDS 41493211 41493498 41493211 ID=cds.MS.gene63213.t1;Parent=MS.gene63213.t1
Gene Sequence

>MS.gene63213

ATGGCTGATTCAAAAATCAAACGACGCAGAGAAGAAGAAGAACACGATGGCACCGACTTGATGAGTATTCTACCGGACTCTCTAGTGTGCCACATCCTATCATTCCTCCCAATTGAAACCGCCGTGGCTACCTGTCTTGTTTCTCGCAGATGGCGCCACCTCTGGAAGCATCTTCAGGTCTTCCACTTCTCCGATGACTCGCACTGCTACATCACTCAATTGAAATTGTTCAAAAAATTTGCATTCTTTGTCAACGCTGTGCTATCCCTCCGTACATCCCGTGACATTCGAAAGTTCCGTCTCCACTCTGGCATATCGGATGAACACAACTTTCCTAGCAACTGCATCAACATGTGGATCACTGCCGCCATTGGGCTCCACCTTCAAGAACTGGATCTCGACATAGCCAACCCCGATGGTGACCAAATGCTGATCCTCCCTTCTTCCCTCTTGAATTGCACCAACCTCGTTTCCCTTAGGTCAGCTTTTCAGTTACAAATCAACATTTTTTTTTTTTTTTTTAGTTTTAGTTTTATTCAATTCAACTCATTCAGAAATTAATATATGTCTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTAAGTAGAATGAAATGAAAACAGAAGGGTTTAATTTTGCCCAAAATTAACTTTCATTATATAATATCTGGAAATAGTATTTATGAATTTGTAGATAATGCTAACTTTCATGGTTTCATCTAAACGCTGCAGTCTTGTTGGTGCAATTGAAATGAAAGTTCAACATTCTTCAGTTCATTTTCCATCACTCAAGTGGCTGAAACTCGACATGCGCGTTATGGATTCAGACATTGCCTTTCTCTCTGGCTGCCCCGTTCTCGAAACTTGCATATTTACTTTGAGCCCAAATTTCTGACCAATGTTCCTGTGCCACCTTCCTCCAAAAAGGTGAAATTCAACAATGGAAATTTCTCATGGACTTGCCTTAAAATAAAATCCCATCGTTTGAGGTCTGCCAAAACCACATTGGGCATCATTGGCAACGTGCATAGTGTTGTGGAAGCATATCTTGATGTCTTTTGTCGGCGTGAGAGTGAATTTGTCGACCCTATTCTGAACTGCCTCAGAGATCCCGATAATGCATTACATCTACGGCTGCGTCATTCGACAAAAAAGGTGAAGTTTTATTACTATGATTTATATGTATCTGTATAACTATTTTTGGTATTGATATTGTGCCATCACTTGTTATCATGTACAAGCAGTGGCCACTTCGTGCCCCTGTTATAAATAATCCAGAATTTTGCAATCTACTTCATCTAAAGTTCATTCTTCCCTGCTTCAATTTGAATCTTCTGGGCAACATTCTTGAGCAATGTCATATGCTTCAAGTTCTCATAATTCAAATAAGCAAGGTTTGTTGACTTATCAAGTTTAATTCAATGAATGTTTGGTCTGTCTATTTAGATATTACTCTGTACGCACAAAAATATACATGCGATTCATGGTGTCCCTTGTTGTCGATAGGAAGAACCATCACCTTTAGGAGCATGTGAGCTGCAGTCAACAACAGTTCCTAAATGTCTCGAATCCCATCTCACCTATATTCACATAGAAGGATACCAAGGACTTGAAGAAGAGCTGACATTTGCTGAATATATTTTGCAGAACGGACTTGTTTTGAAGACAATGCTTATTTTTGTTGATATTTCGATGGACATAGCAAACAAGTACCGTTCTCTCAAAAGATTATCCCGTGCACCAAAAGGCTCTGTCATGTGCCAACTTGAATTTGATGCAGATGTGTCTCCTTAA
Protein sequence

>MS.gene63213.t1

MADSKIKRRREEEEHDGTDLMSILPDSLVCHILSFLPIETAVATCLVSRRWRHLWKHLQVFHFSDDSHCYITQLKLFKKFAFFVNAVLSLRTSRDIRKFRLHSGISDEHNFPSNCINMWITAAIGLHLQELDLDIANPDGDQMLILPSSLLNCTNLVSLSSFSITQVAETRHARYGFRHCLSLWLPRSRNLHIYFEPKFLTNVPVPPSSKKVKFNNGNFSWTCLKIKSHRLRSAKTTLGIIGNVHSVVEAYLDVFCRRESEFVDPILNCLRDPDNALHLRLRHSTKKWPLRAPVINNPEFCNLLHLKFILPCFNLNLLGNILEQCHMLQVLIIQISKEEPSPLGACELQSTTVPKCLESHLTYIHIEGYQGLEEELTFAEYILQNGLVLKTMLIFVDISMDIANKYRSLKRLSRAPKGSVMCQLEFDADVSP