AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053312

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053312.t1 MTR_8g062300 86.929 482 34 2 1 453 1 482 0.0 840
MS.gene053312.t1 MTR_8g070950 57.701 461 163 9 1 452 1 438 4.99e-162 464
MS.gene053312.t1 MTR_8g062010 58.391 435 164 9 26 452 9 434 3.99e-158 454
MS.gene053312.t1 MTR_8g062080 53.896 462 185 9 5 453 4 450 1.59e-156 451
MS.gene053312.t1 MTR_8g062310 55.606 437 175 8 16 452 15 432 1.28e-153 443
MS.gene053312.t1 MTR_8g058965 51.380 471 188 9 13 453 2 461 3.35e-143 417
MS.gene053312.t1 MTR_8g469020 50.109 459 187 10 26 452 7 455 1.52e-136 400
MS.gene053312.t1 MTR_4g050250 48.724 431 167 14 7 394 1 420 1.39e-110 333
MS.gene053312.t1 MTR_8g467790 46.632 386 151 8 25 393 147 494 1.14e-97 303
MS.gene053312.t1 MTR_2g083840 40.798 451 229 14 24 448 19 457 9.64e-82 259
MS.gene053312.t1 MTR_8g468810 39.579 475 248 15 1 448 1 463 4.27e-79 253
MS.gene053312.t1 MTR_8g058970 38.669 481 247 15 10 448 3 477 6.40e-78 250
MS.gene053312.t1 MTR_8g062270 39.387 457 229 17 23 447 13 453 2.04e-77 248
MS.gene053312.t1 MTR_8g014260 37.712 472 249 17 1 448 1 451 1.43e-72 236
MS.gene053312.t1 MTR_2g086020 78.873 142 30 0 311 452 178 319 8.42e-72 229
MS.gene053312.t1 MTR_2g086020 58.904 146 55 4 52 196 1 142 1.41e-39 145
MS.gene053312.t1 MTR_5g092960 36.170 470 257 11 9 447 4 461 1.44e-69 228
MS.gene053312.t1 MTR_8g467800 37.712 472 251 14 1 448 1 453 3.16e-68 224
MS.gene053312.t1 MTR_4g032730 34.937 478 255 12 13 447 2 466 1.95e-67 223
MS.gene053312.t1 MTR_8g468840 36.526 449 243 13 26 446 14 448 6.73e-65 216
MS.gene053312.t1 MTR_8g468760 36.865 453 214 15 25 447 8 418 7.04e-65 214
MS.gene053312.t1 MTR_8g445780 37.838 407 217 17 13 401 2 390 2.29e-56 192
MS.gene053312.t1 MTR_8g468810 39.370 381 192 15 5 358 3 371 3.25e-54 185
MS.gene053312.t1 MTR_5g092960 35.809 377 206 9 9 358 4 371 1.73e-47 168
MS.gene053312.t1 MTR_8g077460 32.962 449 206 18 19 448 3 375 3.41e-45 161
MS.gene053312.t1 MTR_8g468810 40.789 304 149 12 5 288 3 295 3.84e-45 160
MS.gene053312.t1 MTR_8g468810 40.656 305 148 12 5 288 3 295 3.92e-45 160
MS.gene053312.t1 MTR_8g468810 39.571 326 166 12 5 310 3 317 8.71e-45 160
MS.gene053312.t1 MTR_8g014160 31.757 444 223 14 26 448 41 425 1.98e-43 158
MS.gene053312.t1 MTR_8g467800 37.615 327 171 11 1 310 1 311 4.17e-35 134
MS.gene053312.t1 MTR_4g024710 34.343 297 173 11 167 448 58 347 1.10e-31 124
MS.gene053312.t1 MTR_3g099800 66.667 87 28 1 360 445 19 105 2.88e-30 113
MS.gene053312.t1 MTR_8g014210 41.398 186 104 5 264 448 50 231 1.37e-28 113
MS.gene053312.t1 MTR_7g098510 26.147 436 255 11 22 448 14 391 2.53e-25 107
MS.gene053312.t1 MTR_5g084070 27.907 430 274 16 25 440 12 419 1.05e-24 106
MS.gene053312.t1 MTR_1g041245 29.452 438 234 17 25 448 10 386 1.52e-24 105
MS.gene053312.t1 MTR_7g056433 28.111 434 245 17 26 448 12 389 6.50e-23 100
MS.gene053312.t1 MTR_3g107100 27.679 448 251 18 20 448 5 398 1.51e-22 99.8
MS.gene053312.t1 MTR_5g080710 27.546 432 280 14 25 440 32 446 7.06e-22 98.2
MS.gene053312.t1 MTR_4g024745 32.746 284 165 11 165 431 10 284 1.05e-21 95.1
MS.gene053312.t1 MTR_1089s0010 25.959 443 247 19 21 448 3 379 4.00e-21 95.1
MS.gene053312.t1 MTR_3g108150 27.778 432 244 15 26 447 13 386 1.28e-20 93.6
MS.gene053312.t1 MTR_2g007450 27.051 451 255 19 23 445 13 417 1.92e-20 93.6
MS.gene053312.t1 MTR_7g098480 27.252 433 250 14 22 447 14 388 1.01e-19 91.7
MS.gene053312.t1 MTR_3g107160 26.634 413 224 16 21 415 5 356 1.09e-19 90.9
MS.gene053312.t1 MTR_5g021550 42.308 156 75 8 20 166 13 162 2.09e-19 85.5
MS.gene053312.t1 MTR_5g073420 25.908 413 214 16 25 419 10 348 6.05e-19 88.6
MS.gene053312.t1 MTR_5g045170 26.790 433 259 16 28 453 16 397 3.52e-18 86.7
MS.gene053312.t1 MTR_2g036470 26.802 444 257 19 18 447 6 395 3.73e-18 86.3
MS.gene053312.t1 MTR_5g008950 24.424 434 246 17 28 448 1 365 1.09e-17 84.7
MS.gene053312.t1 MTR_3g036250 27.107 439 221 21 26 445 5 363 1.25e-17 84.3
MS.gene053312.t1 MTR_2g007400 25.277 451 252 17 25 448 11 403 6.40e-17 82.8
MS.gene053312.t1 MTR_8g014290 37.580 157 77 8 26 167 10 160 1.61e-16 77.0
MS.gene053312.t1 MTR_7g007080 27.021 433 243 18 26 445 4 376 1.73e-16 81.3
MS.gene053312.t1 MTR_3g107120 26.521 411 226 18 26 415 9 364 1.74e-16 81.6
MS.gene053312.t1 MTR_6g022360 24.292 424 252 14 2 419 19 379 1.85e-16 81.3
MS.gene053312.t1 MTR_4g024720 40.141 142 65 8 1 136 1 128 2.91e-16 77.8
MS.gene053312.t1 MTR_5g007410 24.885 434 254 14 28 445 28 405 3.15e-16 80.9
MS.gene053312.t1 MTR_7g115270 26.097 433 262 15 26 445 12 399 3.44e-16 80.5
MS.gene053312.t1 MTR_7g056430 26.168 428 228 16 26 442 9 359 3.93e-16 81.6
MS.gene053312.t1 MTR_7g056420 26.168 428 228 16 26 442 9 359 4.00e-16 81.6
MS.gene053312.t1 MTR_7g055943 25.556 450 248 20 22 448 36 421 5.92e-16 80.1
MS.gene053312.t1 MTR_7g056447 31.707 205 121 8 26 224 9 200 7.74e-16 78.2
MS.gene053312.t1 MTR_2g005990 24.390 451 248 19 25 445 5 392 2.36e-15 77.8
MS.gene053312.t1 MTR_3g023630 27.650 434 241 20 23 448 5 373 3.54e-15 77.4
MS.gene053312.t1 MTR_7g026500 24.379 443 252 15 26 447 14 394 5.96e-15 76.6
MS.gene053312.t1 MTR_2g008310 26.818 440 237 19 25 448 1 371 1.95e-14 75.1
MS.gene053312.t1 MTR_6g022500 25.943 424 213 20 19 415 94 443 2.48e-14 75.1
MS.gene053312.t1 MTR_5g058000 25.977 435 243 18 25 447 7 374 2.95e-14 74.3
MS.gene053312.t1 MTR_8g014230 41.667 84 47 2 358 441 17 98 4.93e-14 68.6
MS.gene053312.t1 MTR_3g467400 31.731 208 117 9 26 217 3 201 1.63e-13 72.0
MS.gene053312.t1 MTR_2g007500 32.039 206 103 8 21 216 9 187 5.62e-13 70.1
MS.gene053312.t1 MTR_7g032380 32.821 195 117 7 25 216 10 193 6.45e-13 70.1
MS.gene053312.t1 MTR_3g040700 26.185 401 214 17 26 414 43 373 1.28e-12 69.7
MS.gene053312.t1 MTR_4g056020 24.480 433 258 16 26 417 9 413 1.64e-12 69.7
MS.gene053312.t1 MTR_4g056030 33.005 203 116 8 26 220 9 199 2.21e-12 69.3
MS.gene053312.t1 MTR_4g080590 24.890 454 282 17 26 445 23 451 6.56e-12 67.8
MS.gene053312.t1 MTR_8g085430 32.843 204 112 10 25 216 10 200 8.11e-12 66.6
MS.gene053312.t1 MTR_8g468910 31.959 194 110 8 7 191 29 209 1.04e-11 67.0
MS.gene053312.t1 MTR_4g024770 50.000 72 26 2 1 72 8 69 3.05e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053312.t1 AT4G10400 27.002 437 276 17 25 447 1 408 1.07e-21 97.1
MS.gene053312.t1 AT4G10400 27.002 437 276 17 25 447 1 408 1.07e-21 97.1
MS.gene053312.t1 AT4G10400 27.002 437 276 17 25 447 1 408 1.07e-21 97.1
MS.gene053312.t1 AT5G56420 26.281 449 273 15 26 448 6 422 4.20e-20 92.4
MS.gene053312.t1 AT5G56420 26.281 449 273 15 26 448 6 422 4.20e-20 92.4
MS.gene053312.t1 AT5G56420 26.281 449 273 15 26 448 6 422 4.20e-20 92.4
MS.gene053312.t1 AT1G78750 24.947 473 295 19 12 445 4 455 4.17e-19 89.7
MS.gene053312.t1 AT1G78750 24.947 473 295 19 12 445 4 455 4.17e-19 89.7
MS.gene053312.t1 AT4G26340 27.703 444 276 16 25 448 1 419 8.16e-18 85.9
MS.gene053312.t1 AT5G56440 26.247 461 270 15 25 447 1 429 1.94e-17 84.7
MS.gene053312.t1 AT5G56560 27.854 438 273 20 28 448 7 418 7.01e-17 82.8
MS.gene053312.t1 AT3G50710 26.201 458 273 17 25 448 1 427 8.84e-17 82.8
MS.gene053312.t1 AT1G69630 25.052 483 279 17 10 445 2 448 1.97e-16 81.6
MS.gene053312.t1 AT5G53840 25.527 474 269 19 15 445 5 437 3.32e-16 80.9
MS.gene053312.t1 AT1G66290 25.261 479 290 27 2 445 5 450 9.90e-16 79.7
MS.gene053312.t1 AT3G42770 26.818 440 272 13 28 440 1 417 1.14e-15 79.7
MS.gene053312.t1 AT5G22730 25.107 466 273 17 26 445 28 463 1.99e-14 75.5
MS.gene053312.t1 AT5G22730 25.107 466 273 17 26 445 28 463 1.99e-14 75.5
MS.gene053312.t1 AT3G59160 29.778 225 118 9 19 214 8 221 4.00e-14 74.7
MS.gene053312.t1 AT4G15060 30.233 215 133 7 7 216 9 211 8.06e-14 73.6
MS.gene053312.t1 AT5G44940 23.853 436 260 15 24 448 3 377 8.78e-14 73.2
MS.gene053312.t1 AT5G50270 25.587 426 260 12 25 420 1 399 9.01e-14 73.2
MS.gene053312.t1 AT1G66300 24.845 483 290 22 2 445 5 453 1.14e-13 73.2
MS.gene053312.t1 AT4G09920 29.384 211 137 7 25 233 1 201 4.01e-13 70.5
MS.gene053312.t1 AT4G09920 29.384 211 137 7 25 233 1 201 4.01e-13 70.5
MS.gene053312.t1 AT4G09920 29.167 216 141 7 25 238 1 206 4.42e-13 70.1
MS.gene053312.t1 AT4G09920 29.167 216 141 7 25 238 1 206 4.42e-13 70.1
MS.gene053312.t1 AT4G09920 29.167 216 141 7 25 238 1 206 4.42e-13 70.1
MS.gene053312.t1 AT1G78760 24.217 479 280 22 19 449 2 445 5.42e-13 71.2
MS.gene053312.t1 AT5G22610 25.055 451 274 16 26 446 18 434 8.04e-13 70.5
MS.gene053312.t1 AT1G66310 25.212 472 282 21 10 445 3 439 1.59e-12 69.7
MS.gene053312.t1 AT4G15060 30.808 198 122 6 24 216 170 357 1.86e-12 69.7
MS.gene053312.t1 AT2G42720 31.795 195 123 5 25 214 1 190 1.99e-12 69.3
MS.gene053312.t1 AT1G51370 25.926 459 270 18 17 443 10 430 2.03e-12 69.3
MS.gene053312.t1 AT5G22610 24.464 466 288 16 26 446 18 464 2.43e-12 69.3
MS.gene053312.t1 AT3G59190 30.348 201 117 9 26 214 12 201 2.85e-12 68.9
MS.gene053312.t1 AT3G59190 30.288 208 120 10 19 214 7 201 4.00e-12 68.2
MS.gene053312.t1 AT1G13780 24.353 464 286 18 26 445 10 452 5.22e-12 68.2
MS.gene053312.t1 AT5G56820 26.256 438 276 17 26 445 15 423 5.51e-12 67.8
MS.gene053312.t1 AT3G59170 30.455 220 113 9 26 214 7 217 6.67e-12 67.8
MS.gene053312.t1 AT3G26922 27.941 272 170 7 17 285 5 253 8.97e-12 66.2
MS.gene053312.t1 AT3G26922 27.941 272 170 7 17 285 5 253 8.97e-12 66.2
MS.gene053312.t1 AT3G59200 28.643 199 120 6 26 214 7 193 9.81e-12 66.6
MS.gene053312.t1 AT4G14103 28.571 196 122 6 26 214 8 192 1.09e-11 67.0
MS.gene053312.t1 AT5G38390 30.667 225 131 7 25 245 1 204 1.39e-11 66.6
MS.gene053312.t1 AT4G14103 28.571 196 122 6 26 214 8 192 1.44e-11 66.2
MS.gene053312.t1 AT4G14103 28.571 196 122 6 26 214 8 192 1.44e-11 66.2
MS.gene053312.t1 AT3G59200 28.643 199 120 6 26 214 7 193 2.14e-11 66.2
MS.gene053312.t1 AT1G16930 30.808 198 113 10 26 216 15 195 3.58e-11 65.5
MS.gene053312.t1 AT1G16930 30.808 198 113 10 26 216 15 195 3.58e-11 65.5
MS.gene053312.t1 AT1G16930 30.808 198 113 10 26 216 15 195 3.58e-11 65.5

Find 110 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAAACACTGCAGTCTTTC+TGG 0.129894 8.3:-39418457 MS.gene053312:intron
AAACGAAGGATATATTAATT+TGG 0.153932 8.3:-39418188 MS.gene053312:CDS
CCCCAAGCTGGAAACTTTGC+AGG 0.229332 8.3:-39418320 MS.gene053312:CDS
GATTGGCCGGTTTCGAGCTC+TGG 0.257085 8.3:+39418239 None:intergenic
GTCTCTCGGCACAGAAATTA+TGG 0.288190 8.3:-39418377 MS.gene053312:CDS
ACCTGCAAAGTTTCCAGCTT+GGG 0.309530 8.3:+39418319 None:intergenic
AACGAAGGATATATTAATTT+GGG 0.327225 8.3:-39418187 MS.gene053312:CDS
CTCGCCGGTGGCGCCACCTC+TGG 0.341008 8.3:-39419127 MS.gene053312:CDS
GAGATGGAGGTGTTGAAGTT+GGG 0.349818 8.3:+39418890 None:intergenic
CTGACCTATGTTCACATAGA+AGG 0.351633 8.3:-39417582 MS.gene053312:CDS
TATATTAATTTGGGCATTGT+AGG 0.357147 8.3:-39418178 MS.gene053312:CDS
ATGTTTCAACTCAAATCTTC+TGG 0.358687 8.3:-39417857 MS.gene053312:CDS
ACTAGGAGGTGGTCGAGAAC+AGG 0.375934 8.3:+39418088 None:intergenic
TTCCAACTAAAACCTCTGTT+TGG 0.387272 8.3:-39419166 MS.gene053312:CDS
GTAGACGGTAACAGTAGAAT+TGG 0.397098 8.3:+39419087 None:intergenic
AGAGATGGAGGTGTTGAAGT+TGG 0.410070 8.3:+39418889 None:intergenic
TTGGAAGGAAGGACATGATA+TGG 0.412054 8.3:+39419183 None:intergenic
TGTAGGCAACTTTCACACTA+TGG 0.425217 8.3:-39418161 MS.gene053312:CDS
AAATTACTTTCCTTCCTCTC+TGG 0.429603 8.3:-39418346 MS.gene053312:CDS
CCTCTCTGGCTGCCCCAAGC+TGG 0.444251 8.3:-39418332 MS.gene053312:CDS
GCTCCAACCTCCTTTCCCTT+AGG 0.450370 8.3:-39418815 MS.gene053312:intron
AATTCAGGATAATTTAGAAC+AGG 0.454737 8.3:+39417913 None:intergenic
CCTTCCAGAGGTGGCGCCAC+CGG 0.463852 8.3:+39419123 None:intergenic
CCGTAGATTTATAGCATCAT+GGG 0.467450 8.3:+39418063 None:intergenic
CGGCCAATCATAATTTCACT+TGG 0.470608 8.3:-39418225 MS.gene053312:CDS
CAATTGATGAAGAGGGAAGG+AGG 0.470818 8.3:+39418837 None:intergenic
AGTCCAAGTGAAATTATGAT+TGG 0.480791 8.3:+39418222 None:intergenic
TTATAGCATCATGGGGAACT+AGG 0.481247 8.3:+39418071 None:intergenic
AATTGATGAAGAGGGAAGGA+GGG 0.486231 8.3:+39418838 None:intergenic
AACCTGCAAAGTTTCCAGCT+TGG 0.491460 8.3:+39418318 None:intergenic
GACCCAACTGTATCCTCATA+AGG 0.492983 8.3:-39417384 None:intergenic
ATTGGCCGGTTTCGAGCTCT+GGG 0.493701 8.3:+39418240 None:intergenic
ACCGTAGATTTATAGCATCA+TGG 0.495398 8.3:+39418062 None:intergenic
AACAGTAGAATTGGAAGACT+TGG 0.497863 8.3:+39419096 None:intergenic
AAGATTAACAAATATATCAA+AGG 0.513545 8.3:-39417433 MS.gene053312:CDS
TTCCTCCCAGAGCTCGAAAC+CGG 0.515568 8.3:-39418245 MS.gene053312:CDS
AACTCTTCTGCTGATTTCTG+TGG 0.519465 8.3:+39419269 None:intergenic
ATATGGCACAGGAGTGTGTT+CGG 0.519601 8.3:+39419200 None:intergenic
TACCTTATGAGGATACAGTT+GGG 0.528201 8.3:+39417382 None:intergenic
TGAATGGAATTGAATGTAGA+CGG 0.528341 8.3:+39419072 None:intergenic
CTTGGGGCAGCCAGAGAGGA+AGG 0.529957 8.3:+39418336 None:intergenic
GGTTGGAGCAATTGATGAAG+AGG 0.530052 8.3:+39418829 None:intergenic
TTCGAGCTCTGGGAGGAAGA+TGG 0.530767 8.3:+39418250 None:intergenic
CGATGAGCCTCGTCTCTCGC+CGG 0.533455 8.3:-39419142 MS.gene053312:CDS
GTTCACATAGAAGGGTATCA+AGG 0.534998 8.3:-39417573 MS.gene053312:CDS
CGACAAATTCACTCTCAAAA+CGG 0.535261 8.3:+39418113 None:intergenic
AGATTAACAAATATATCAAA+GGG 0.542609 8.3:-39417432 MS.gene053312:CDS
CAAGTGAAATTATGATTGGC+CGG 0.542813 8.3:+39418226 None:intergenic
CTTCTATGTGAACATAGGTC+AGG 0.542971 8.3:+39417583 None:intergenic
GTGTTGAAGTTGGGGTCCAA+TGG 0.543943 8.3:+39418899 None:intergenic
TCCGGTGTCCATGTTCTCAA+TGG 0.544353 8.3:+39417639 None:intergenic
CCCATGATGCTATAAATCTA+CGG 0.544523 8.3:-39418063 MS.gene053312:CDS
TGATGAAGAGGGAAGGAGGG+AGG 0.545199 8.3:+39418841 None:intergenic
AACCACCACCATTGAGAACA+TGG 0.546378 8.3:-39417647 MS.gene053312:CDS
CCGGTGGCGCCACCTCTGGA+AGG 0.547084 8.3:-39419123 MS.gene053312:CDS
CAGTTGGGTCGAACTTAAGT+TGG 0.547514 8.3:+39417397 None:intergenic
TTACCTAAGGGAAAGGAGGT+TGG 0.550204 8.3:+39418812 None:intergenic
CCAGCTTGGGGCAGCCAGAG+AGG 0.557168 8.3:+39418332 None:intergenic
TTACCTTATGAGGATACAGT+TGG 0.557869 8.3:+39417381 None:intergenic
GTTGGAGCAATTGATGAAGA+GGG 0.561648 8.3:+39418830 None:intergenic
GCATTCGTCACAAAGACTGC+AGG 0.566927 8.3:+39418280 None:intergenic
GTTGGGGTCCAATGGCATCA+AGG 0.569817 8.3:+39418907 None:intergenic
TCGTGGATCCTTGATGCCAT+TGG 0.570896 8.3:-39418915 MS.gene053312:CDS
TCAGAAACTGTTGTTGACTC+CGG 0.578291 8.3:+39417621 None:intergenic
ATACCCTTCTATGTGAACAT+AGG 0.580469 8.3:+39417578 None:intergenic
AGATGGAGGTGTTGAAGTTG+GGG 0.580903 8.3:+39418891 None:intergenic
GTCCATGTTCTCAATGGTGG+TGG 0.584631 8.3:+39417645 None:intergenic
GAGCAATTGATGAAGAGGGA+AGG 0.585056 8.3:+39418834 None:intergenic
AGAAGATTTGAGTTGAAACA+TGG 0.586977 8.3:+39417859 None:intergenic
CCTGCAAAGTTTCCAGCTTG+GGG 0.588595 8.3:+39418320 None:intergenic
CTATGTGAACATAGGTCAGG+TGG 0.588925 8.3:+39417586 None:intergenic
TGACCTATGTTCACATAGAA+GGG 0.590318 8.3:-39417581 MS.gene053312:CDS
AGAGACAACATCTTCAGTGA+TGG 0.590452 8.3:+39418394 None:intergenic
GGAAGGACATGATATGGCAC+AGG 0.590863 8.3:+39419189 None:intergenic
TGCGGAAACTGCGAAGGTGA+AGG 0.596603 8.3:+39419021 None:intergenic
CATCAATGTGAATATAGAGA+TGG 0.599695 8.3:+39418874 None:intergenic
CTAGGAGGTGGTCGAGAACA+GGG 0.599798 8.3:+39418089 None:intergenic
TGAGCCTCGTCTCTCGCCGG+TGG 0.605450 8.3:-39419139 MS.gene053312:CDS
TGAGAGGTTACCTAAGGGAA+AGG 0.607320 8.3:+39418805 None:intergenic
CATCATGGGGAACTAGGAGG+TGG 0.608053 8.3:+39418077 None:intergenic
TGCGGCATTTCTCAAGCATG+TGG 0.611685 8.3:+39417833 None:intergenic
TCACTGAAGATGTTGTCTCT+CGG 0.611749 8.3:-39418391 MS.gene053312:CDS
GGCGCCACCGGCGAGAGACG+AGG 0.612718 8.3:+39419135 None:intergenic
CAATGTGAATATAGAGATGG+AGG 0.614846 8.3:+39418877 None:intergenic
GATGGCAGAATCAAGAAGCA+TGG 0.615963 8.3:-39419294 MS.gene053312:CDS
CTTGGAGATCCTTCCAGAGG+TGG 0.619949 8.3:+39419114 None:intergenic
TTGTCCACTGTGTTGAATCG+TGG 0.624204 8.3:-39418932 MS.gene053312:CDS
GTTGCGTCATTCGACATCAA+AGG 0.630198 8.3:-39418041 MS.gene053312:intron
GAGTTAGTAAACGATGGCGG+AGG 0.630834 8.3:-39419251 MS.gene053312:CDS
TATGTGAACATAGGTCAGGT+GGG 0.643030 8.3:+39417587 None:intergenic
GGTGTCCATGTTCTCAATGG+TGG 0.646260 8.3:+39417642 None:intergenic
ACCATTGAGAACATGGACAC+CGG 0.646446 8.3:-39417640 MS.gene053312:CDS
GATGTAACTTATGAATGTTG+CGG 0.648336 8.3:+39419003 None:intergenic
GGCCGGTTTCGAGCTCTGGG+AGG 0.664051 8.3:+39418243 None:intergenic
ACTTACTTCGATATAAACGA+AGG 0.665297 8.3:-39418202 MS.gene053312:CDS
TCTTTCTGGTAAGATCAGCA+TGG 0.667331 8.3:-39418443 MS.gene053312:CDS
GAATGTTGCGGAAACTGCGA+AGG 0.667547 8.3:+39419015 None:intergenic
TTATGAGAACTTGAAGCATG+CGG 0.670506 8.3:+39417815 None:intergenic
AGGCAACTTTCACACTATGG+AGG 0.670571 8.3:-39418158 MS.gene053312:CDS
GAGGTTACCTAAGGGAAAGG+AGG 0.670966 8.3:+39418808 None:intergenic
CGTAGATTTATAGCATCATG+GGG 0.673823 8.3:+39418064 None:intergenic
GCAGAAGAGTTAGTAAACGA+TGG 0.674951 8.3:-39419257 MS.gene053312:CDS
TCTCATAATTCAGAGCAACA+AGG 0.677248 8.3:-39417800 MS.gene053312:intron
TAGCATCATGGGGAACTAGG+AGG 0.681420 8.3:+39418074 None:intergenic
AGACTTGGAGATCCTTCCAG+AGG 0.695151 8.3:+39419111 None:intergenic
GAGGCTCATCGTCCAAACAG+AGG 0.696258 8.3:+39419154 None:intergenic
TTCTGGTAAGATCAGCATGG+AGG 0.697387 8.3:-39418440 MS.gene053312:CDS
GAAGAGTTAGTAAACGATGG+CGG 0.698804 8.3:-39419254 MS.gene053312:CDS
TTTAATGGACAAAACAGACA+AGG 0.711577 8.3:-39417469 MS.gene053312:CDS
GGATCCACGATTCAACACAG+TGG 0.768479 8.3:+39418928 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATATTAGTAATTTGA+AGG + chr8.3:39418131-39418150 None:intergenic 10.0%
!!! CTATATTTGTTATAAAAAAA+AGG + chr8.3:39418176-39418195 None:intergenic 10.0%
!! AAGATTAACAAATATATCAA+AGG - chr8.3:39419246-39419265 MS.gene053312:CDS 15.0%
!! AGATTAACAAATATATCAAA+GGG - chr8.3:39419247-39419266 MS.gene053312:CDS 15.0%
!!! AATAATAGAAACTTTTTGAA+TGG + chr8.3:39417626-39417645 None:intergenic 15.0%
!!! TATTTTTGTTGATAGTTTAA+TGG - chr8.3:39419195-39419214 MS.gene053312:CDS 15.0%
!! AAACGAAGGATATATTAATT+TGG - chr8.3:39418491-39418510 MS.gene053312:intron 20.0%
!! AACGAAGGATATATTAATTT+GGG - chr8.3:39418492-39418511 MS.gene053312:intron 20.0%
!! CATAATATACAGTAATAAGA+AGG + chr8.3:39417922-39417941 None:intergenic 20.0%
!! GTCATTACTCAAAATTATAT+TGG + chr8.3:39418985-39419004 None:intergenic 20.0%
!!! TATAATTTTGAGTAATGACA+TGG - chr8.3:39418986-39419005 MS.gene053312:CDS 20.0%
! TGAGCATAAAAACAAATGAT+TGG + chr8.3:39417992-39418011 None:intergenic 25.0%
!! AATTCAGGATAATTTAGAAC+AGG + chr8.3:39418769-39418788 None:intergenic 25.0%
!! TATATTAATTTGGGCATTGT+AGG - chr8.3:39418501-39418520 MS.gene053312:intron 25.0%
!!! ATTGGTTTTGTCATTCAAAA+AGG - chr8.3:39418077-39418096 MS.gene053312:CDS 25.0%
AAAATTGATACTGCAATGAG+AGG + chr8.3:39417893-39417912 None:intergenic 30.0%
ACATGCATGTATATATTGAC+AGG + chr8.3:39418943-39418962 None:intergenic 30.0%
ACTTACTTCGATATAAACGA+AGG - chr8.3:39418477-39418496 MS.gene053312:intron 30.0%
AGTCCAAGTGAAATTATGAT+TGG + chr8.3:39418460-39418479 None:intergenic 30.0%
ATAGCAATGTTGTGTCAATT+AGG - chr8.3:39418030-39418049 MS.gene053312:intron 30.0%
ATGTTTCAACTCAAATCTTC+TGG - chr8.3:39418822-39418841 MS.gene053312:CDS 30.0%
CATCAATGTGAATATAGAGA+TGG + chr8.3:39417808-39417827 None:intergenic 30.0%
GAGATATGAGTTGTCAAAAA+CGG + chr8.3:39417723-39417742 None:intergenic 30.0%
GATGTAACTTATGAATGTTG+CGG + chr8.3:39417679-39417698 None:intergenic 30.0%
TGAATGGAATTGAATGTAGA+CGG + chr8.3:39417610-39417629 None:intergenic 30.0%
TGTCATTCAAAAAGGATTAG+CGG - chr8.3:39418085-39418104 MS.gene053312:CDS 30.0%
! AAGGTGAGGTTATACTTTTA+TGG - chr8.3:39418657-39418676 MS.gene053312:intron 30.0%
! GCTGAATATATTTTGCAGAA+CGG - chr8.3:39419151-39419170 MS.gene053312:CDS 30.0%
! TTTAATGGACAAAACAGACA+AGG - chr8.3:39419210-39419229 MS.gene053312:CDS 30.0%
! TTTTATGCTCAAACCGAAAA+GGG - chr8.3:39418001-39418020 MS.gene053312:intron 30.0%
! TTTTGTAAATCGCGAAATTC+AGG + chr8.3:39418784-39418803 None:intergenic 30.0%
! TTTTTATGCTCAAACCGAAA+AGG - chr8.3:39418000-39418019 MS.gene053312:intron 30.0%
!! AGAAGATTTGAGTTGAAACA+TGG + chr8.3:39418823-39418842 None:intergenic 30.0%
AAATTACTTTCCTTCCTCTC+TGG - chr8.3:39418333-39418352 MS.gene053312:CDS 35.0%
AACAGTAGAATTGGAAGACT+TGG + chr8.3:39417586-39417605 None:intergenic 35.0%
ACCGTAGATTTATAGCATCA+TGG + chr8.3:39418620-39418639 None:intergenic 35.0%
ATACCCTTCTATGTGAACAT+AGG + chr8.3:39419104-39419123 None:intergenic 35.0%
CAAGTGAAATTATGATTGGC+CGG + chr8.3:39418456-39418475 None:intergenic 35.0%
CAATGTGAATATAGAGATGG+AGG + chr8.3:39417805-39417824 None:intergenic 35.0%
CCCATGATGCTATAAATCTA+CGG - chr8.3:39418616-39418635 MS.gene053312:intron 35.0%
CCGTAGATTTATAGCATCAT+GGG + chr8.3:39418619-39418638 None:intergenic 35.0%
CGACAAATTCACTCTCAAAA+CGG + chr8.3:39418569-39418588 None:intergenic 35.0%
CGTAGATTTATAGCATCATG+GGG + chr8.3:39418618-39418637 None:intergenic 35.0%
TCTCATAATTCAGAGCAACA+AGG - chr8.3:39418879-39418898 MS.gene053312:CDS 35.0%
TGACCTATGTTCACATAGAA+GGG - chr8.3:39419098-39419117 MS.gene053312:CDS 35.0%
TTATGAGAACTTGAAGCATG+CGG + chr8.3:39418867-39418886 None:intergenic 35.0%
TTCCAACTAAAACCTCTGTT+TGG - chr8.3:39417513-39417532 MS.gene053312:CDS 35.0%
!! TTGCTATTCATGACCCTTTT+CGG + chr8.3:39418017-39418036 None:intergenic 35.0%
!!! AAACAGAGGTTTTAGTTGGA+AGG + chr8.3:39417514-39417533 None:intergenic 35.0%
AATTGATGAAGAGGGAAGGA+GGG + chr8.3:39417844-39417863 None:intergenic 40.0%
AGAGACAACATCTTCAGTGA+TGG + chr8.3:39418288-39418307 None:intergenic 40.0%
AGTGTTTACCAAAGACTGAC+AGG + chr8.3:39418212-39418231 None:intergenic 40.0%
CGGCCAATCATAATTTCACT+TGG - chr8.3:39418454-39418473 MS.gene053312:intron 40.0%
CTGACCTATGTTCACATAGA+AGG - chr8.3:39419097-39419116 MS.gene053312:CDS 40.0%
CTTCTATGTGAACATAGGTC+AGG + chr8.3:39419099-39419118 None:intergenic 40.0%
CTTTCGACGGTCTATTTAAG+AGG - chr8.3:39418054-39418073 MS.gene053312:CDS 40.0%
GAAGAGTTAGTAAACGATGG+CGG - chr8.3:39417425-39417444 MS.gene053312:CDS 40.0%
GACATGGTTTCCTTTATTGC+TGG - chr8.3:39419002-39419021 MS.gene053312:CDS 40.0%
GACGGTCTATTTAAGAGGAT+TGG - chr8.3:39418059-39418078 MS.gene053312:CDS 40.0%
GCAGAAGAGTTAGTAAACGA+TGG - chr8.3:39417422-39417441 MS.gene053312:CDS 40.0%
GTAGACGGTAACAGTAGAAT+TGG + chr8.3:39417595-39417614 None:intergenic 40.0%
GTCAAAAACGGAACGATGAA+AGG + chr8.3:39417711-39417730 None:intergenic 40.0%
GTGTCAATTAGGTCTTTCGA+CGG - chr8.3:39418041-39418060 MS.gene053312:intron 40.0%
GTTGGAGCAATTGATGAAGA+GGG + chr8.3:39417852-39417871 None:intergenic 40.0%
TACATTGACCTGTCAGTCTT+TGG - chr8.3:39418201-39418220 MS.gene053312:CDS 40.0%
TATGTGAACATAGGTCAGGT+GGG + chr8.3:39419095-39419114 None:intergenic 40.0%
TCACTGAAGATGTTGTCTCT+CGG - chr8.3:39418288-39418307 MS.gene053312:CDS 40.0%
TGCAATGAGAGGTTACCTAA+GGG + chr8.3:39417882-39417901 None:intergenic 40.0%
TGGTTTCCTTTATTGCTGGT+AGG - chr8.3:39419006-39419025 MS.gene053312:CDS 40.0%
TGTAGGCAACTTTCACACTA+TGG - chr8.3:39418518-39418537 MS.gene053312:intron 40.0%
TTATAGCATCATGGGGAACT+AGG + chr8.3:39418611-39418630 None:intergenic 40.0%
TTGACAGGTCAGAGATTGTT+TGG + chr8.3:39418928-39418947 None:intergenic 40.0%
TTGGAAGGAAGGACATGATA+TGG + chr8.3:39417499-39417518 None:intergenic 40.0%
TTTCCTTTATTGCTGGTAGG+AGG - chr8.3:39419009-39419028 MS.gene053312:CDS 40.0%
! TCTTTCTGGTAAGATCAGCA+TGG - chr8.3:39418236-39418255 MS.gene053312:CDS 40.0%
!! AACTCTTCTGCTGATTTCTG+TGG + chr8.3:39417413-39417432 None:intergenic 40.0%
!! GTCCAAACAGAGGTTTTAGT+TGG + chr8.3:39417518-39417537 None:intergenic 40.0%
!! GTTCACATAGAAGGGTATCA+AGG - chr8.3:39419106-39419125 MS.gene053312:CDS 40.0%
!! TCAGAAACTGTTGTTGACTC+CGG + chr8.3:39419061-39419080 None:intergenic 40.0%
!!! AGAGGTTTTAGTTGGAAGGA+AGG + chr8.3:39417510-39417529 None:intergenic 40.0%
AACCACCACCATTGAGAACA+TGG - chr8.3:39419032-39419051 MS.gene053312:CDS 45.0%
AACCTGCAAAGTTTCCAGCT+TGG + chr8.3:39418364-39418383 None:intergenic 45.0%
ACCATTGAGAACATGGACAC+CGG - chr8.3:39419039-39419058 MS.gene053312:CDS 45.0%
ACCTGCAAAGTTTCCAGCTT+GGG + chr8.3:39418363-39418382 None:intergenic 45.0%
AGGCAACTTTCACACTATGG+AGG - chr8.3:39418521-39418540 MS.gene053312:intron 45.0%
CAATTGATGAAGAGGGAAGG+AGG + chr8.3:39417845-39417864 None:intergenic 45.0%
CAGTTGGGTCGAACTTAAGT+TGG + chr8.3:39419285-39419304 None:intergenic 45.0%
CTATGTGAACATAGGTCAGG+TGG + chr8.3:39419096-39419115 None:intergenic 45.0%
CTGCAATGAGAGGTTACCTA+AGG + chr8.3:39417883-39417902 None:intergenic 45.0%
GAGCAATTGATGAAGAGGGA+AGG + chr8.3:39417848-39417867 None:intergenic 45.0%
GGTAAACACTGCAGTCTTTC+TGG - chr8.3:39418222-39418241 MS.gene053312:CDS 45.0%
GGTTGGAGCAATTGATGAAG+AGG + chr8.3:39417853-39417872 None:intergenic 45.0%
GTCATTCGACATCAAAGGTG+AGG - chr8.3:39418643-39418662 MS.gene053312:intron 45.0%
GTCTCTCGGCACAGAAATTA+TGG - chr8.3:39418302-39418321 MS.gene053312:CDS 45.0%
GTTCCTCCTACCAGCAATAA+AGG + chr8.3:39419015-39419034 None:intergenic 45.0%
GTTGCGTCATTCGACATCAA+AGG - chr8.3:39418638-39418657 MS.gene053312:intron 45.0%
TGAGAGGTTACCTAAGGGAA+AGG + chr8.3:39417877-39417896 None:intergenic 45.0%
TTACCTAAGGGAAAGGAGGT+TGG + chr8.3:39417870-39417889 None:intergenic 45.0%
TTCTGGTAAGATCAGCATGG+AGG - chr8.3:39418239-39418258 MS.gene053312:CDS 45.0%
TTGTCCACTGTGTTGAATCG+TGG - chr8.3:39417747-39417766 MS.gene053312:intron 45.0%
! ATATGGCACAGGAGTGTGTT+CGG + chr8.3:39417482-39417501 None:intergenic 45.0%
!! AGAGATGGAGGTGTTGAAGT+TGG + chr8.3:39417793-39417812 None:intergenic 45.0%
!! AGATGGAGGTGTTGAAGTTG+GGG + chr8.3:39417791-39417810 None:intergenic 45.0%
!! GAGATGGAGGTGTTGAAGTT+GGG + chr8.3:39417792-39417811 None:intergenic 45.0%
!!! TGCAGAACGGACTTGTTTTG+AGG - chr8.3:39419164-39419183 MS.gene053312:CDS 45.0%
AGACTTGGAGATCCTTCCAG+AGG + chr8.3:39417571-39417590 None:intergenic 50.0%
CCTGCAAAGTTTCCAGCTTG+GGG + chr8.3:39418362-39418381 None:intergenic 50.0%
GAATGTTGCGGAAACTGCGA+AGG + chr8.3:39417667-39417686 None:intergenic 50.0%
GAGGTTACCTAAGGGAAAGG+AGG + chr8.3:39417874-39417893 None:intergenic 50.0%
GCATTCGTCACAAAGACTGC+AGG + chr8.3:39418402-39418421 None:intergenic 50.0%
GGAAGGACATGATATGGCAC+AGG + chr8.3:39417493-39417512 None:intergenic 50.0%
GGATCCACGATTCAACACAG+TGG + chr8.3:39417754-39417773 None:intergenic 50.0%
GGTGTCCATGTTCTCAATGG+TGG + chr8.3:39419040-39419059 None:intergenic 50.0%
GTCCATGTTCTCAATGGTGG+TGG + chr8.3:39419037-39419056 None:intergenic 50.0%
GTGTTGAAGTTGGGGTCCAA+TGG + chr8.3:39417783-39417802 None:intergenic 50.0%
TAGCATCATGGGGAACTAGG+AGG + chr8.3:39418608-39418627 None:intergenic 50.0%
TCCGGTGTCCATGTTCTCAA+TGG + chr8.3:39419043-39419062 None:intergenic 50.0%
TCGTGGATCCTTGATGCCAT+TGG - chr8.3:39417764-39417783 MS.gene053312:intron 50.0%
TGCGGCATTTCTCAAGCATG+TGG + chr8.3:39418849-39418868 None:intergenic 50.0%
! GAGTTAGTAAACGATGGCGG+AGG - chr8.3:39417428-39417447 MS.gene053312:CDS 50.0%
ACTAGGAGGTGGTCGAGAAC+AGG + chr8.3:39418594-39418613 None:intergenic 55.0%
CATCATGGGGAACTAGGAGG+TGG + chr8.3:39418605-39418624 None:intergenic 55.0%
CCCCAAGCTGGAAACTTTGC+AGG - chr8.3:39418359-39418378 MS.gene053312:CDS 55.0%
CTAGGAGGTGGTCGAGAACA+GGG + chr8.3:39418593-39418612 None:intergenic 55.0%
CTTGGAGATCCTTCCAGAGG+TGG + chr8.3:39417568-39417587 None:intergenic 55.0%
GAGGCTCATCGTCCAAACAG+AGG + chr8.3:39417528-39417547 None:intergenic 55.0%
GCTCCAACCTCCTTTCCCTT+AGG - chr8.3:39417864-39417883 MS.gene053312:CDS 55.0%
GTTGGGGTCCAATGGCATCA+AGG + chr8.3:39417775-39417794 None:intergenic 55.0%
TGATGAAGAGGGAAGGAGGG+AGG + chr8.3:39417841-39417860 None:intergenic 55.0%
TGCGGAAACTGCGAAGGTGA+AGG + chr8.3:39417661-39417680 None:intergenic 55.0%
TTCCTCCCAGAGCTCGAAAC+CGG - chr8.3:39418434-39418453 MS.gene053312:CDS 55.0%
! ATTGGCCGGTTTCGAGCTCT+GGG + chr8.3:39418442-39418461 None:intergenic 55.0%
!! TTCGAGCTCTGGGAGGAAGA+TGG + chr8.3:39418432-39418451 None:intergenic 55.0%
! GATTGGCCGGTTTCGAGCTC+TGG + chr8.3:39418443-39418462 None:intergenic 60.0%
CGATGAGCCTCGTCTCTCGC+CGG - chr8.3:39417537-39417556 MS.gene053312:CDS 65.0%
CTTGGGGCAGCCAGAGAGGA+AGG + chr8.3:39418346-39418365 None:intergenic 65.0%
CCAGCTTGGGGCAGCCAGAG+AGG + chr8.3:39418350-39418369 None:intergenic 70.0%
CCTCTCTGGCTGCCCCAAGC+TGG - chr8.3:39418347-39418366 MS.gene053312:CDS 70.0%
CCTTCCAGAGGTGGCGCCAC+CGG + chr8.3:39417559-39417578 None:intergenic 70.0%
TGAGCCTCGTCTCTCGCCGG+TGG - chr8.3:39417540-39417559 MS.gene053312:CDS 70.0%
!! GGCCGGTTTCGAGCTCTGGG+AGG + chr8.3:39418439-39418458 None:intergenic 70.0%
CCGGTGGCGCCACCTCTGGA+AGG - chr8.3:39417556-39417575 MS.gene053312:CDS 75.0%
! CTCGCCGGTGGCGCCACCTC+TGG - chr8.3:39417552-39417571 MS.gene053312:CDS 80.0%
! GGCGCCACCGGCGAGAGACG+AGG + chr8.3:39417547-39417566 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 39417386 39419315 39417386 ID=MS.gene053312
chr8.3 mRNA 39417386 39419315 39417386 ID=MS.gene053312.t1;Parent=MS.gene053312
chr8.3 exon 39418816 39419315 39418816 ID=MS.gene053312.t1.exon1;Parent=MS.gene053312.t1
chr8.3 CDS 39418816 39419315 39418816 ID=cds.MS.gene053312.t1;Parent=MS.gene053312.t1
chr8.3 exon 39418042 39418465 39418042 ID=MS.gene053312.t1.exon2;Parent=MS.gene053312.t1
chr8.3 CDS 39418042 39418465 39418042 ID=cds.MS.gene053312.t1;Parent=MS.gene053312.t1
chr8.3 exon 39417801 39417950 39417801 ID=MS.gene053312.t1.exon3;Parent=MS.gene053312.t1
chr8.3 CDS 39417801 39417950 39417801 ID=cds.MS.gene053312.t1;Parent=MS.gene053312.t1
chr8.3 exon 39417386 39417673 39417386 ID=MS.gene053312.t1.exon4;Parent=MS.gene053312.t1
chr8.3 CDS 39417386 39417673 39417386 ID=cds.MS.gene053312.t1;Parent=MS.gene053312.t1
Gene Sequence

>MS.gene053312

ATGGCAGAATCAAGAAGCATGGAACCACAGAAATCAGCAGAAGAGTTAGTAAACGATGGCGGAGGCAATGACATCGACATATTGAGCAGTCTACCGAACACACTCCTGTGCCATATCATGTCCTTCCTTCCAACTAAAACCTCTGTTTGGACGATGAGCCTCGTCTCTCGCCGGTGGCGCCACCTCTGGAAGGATCTCCAAGTCTTCCAATTCTACTGTTACCGTCTACATTCAATTCCATTCAAAAAGTTTCTATTATTCGTCAACACTGTCCTTCACCTTCGCAGTTTCCGCAACATTCATAAGTTACATCTCGATCTCACCTTTCATCGTTCCGTTTTTGACAACTCATATCTCAACTTTGTCCACTGTGTTGAATCGTGGATCCTTGATGCCATTGGACCCCAACTTCAACACCTCCATCTCTATATTCACATTGATGCTGTTATACACCTCCCTCCTTCCCTCTTCATCAATTGCTCCAACCTCCTTTCCCTTAGGTAACCTCTCATTGCAGTATCAATTTTGTTACACCTTCTTATTACTGTATATTATGCACAAAATAATGCTTCACTAAGTTGTAATTACTAAACTCAATAATATCCAATCATTTGTTTTTATGCTCAAACCGAAAAGGGTCATGAATAGCAATGTTGTGTCAATTAGGTCTTTCGACGGTCTATTTAAGAGGATTGGTTTTGTCATTCAAAAAGGATTAGCGGCGCAGTTTGTTGCTTGTTTGCCTTCAAATTACTAATATTATTTTCTTTCATCTATTGTTCTGTTACCTTTTTTTTATAACAAATATAGAATGTTACATTGACCTGTCAGTCTTTGGTAAACACTGCAGTCTTTCTGGTAAGATCAGCATGGAGGTTAAACATTCTTCACTTCATTTTCCATCACTGAAGATGTTGTCTCTCGGCACAGAAATTATGGATGAAAGTAAATTACTTTCCTTCCTCTCTGGCTGCCCCAAGCTGGAAACTTTGCAGGTTTACTTTTACTTTCAACCTGCAGTCTTTGTGACGAATGCTCTCGTTCCATCTTCCTCCCAGAGCTCGAAACCGGCCAATCATAATTTCACTTGGACTTACTTCGATATAAACGAAGGATATATTAATTTGGGCATTGTAGGCAACTTTCACACTATGGAGGAAGCATTTCTCAATGTCTTTTACCGTTTTGAGAGTGAATTTGTCGACCCTGTTCTCGACCACCTCCTAGTTCCCCATGATGCTATAAATCTACGGTTGCGTCATTCGACATCAAAGGTGAGGTTATACTTTTATGGTTTCTATTTTTTTATACGTGTAGTTTCGTACTAATATTCTGCAATCACTTGTTGTTATCGTCTACAAGCAGTGTTCACTTCATGCTCCTGTTCTAAATTATCCTGAATTTCGCGATTTACAAAATCTGAAGTTCATTCTTCCATGTTTCAACTCAAATCTTCTGGTCCACATGCTTGAGAAATGCCGCATGCTTCAAGTTCTCATAATTCAGAGCAACAAGGTATGTTAACTTCTCAATTTTAATCCAAACAATCTCTGACCTGTCAATATATACATGCATGTATGTATCTATCTGTACATACCAATATAATTTTGAGTAATGACATGGTTTCCTTTATTGCTGGTAGGAGGAACCACCACCATTGAGAACATGGACACCGGAGTCAACAACAGTTTCTGAATGTCTCAAATCCCACCTGACCTATGTTCACATAGAAGGGTATCAAGGACTTGAAGATGAGCTGACATTTGCTGAATATATTTTGCAGAACGGACTTGTTTTGAGGACAATGTTTATTTTTGTTGATAGTTTAATGGACAAAACAGACAAGGAATGTTCTCTCAAAAGATTAACAAATATATCAAAGGGCTCTGTCACGTGCCAACTTAAGTTCGACCCAACTGTATCCTCATAA
Protein sequence

>MS.gene053312.t1

MAESRSMEPQKSAEELVNDGGGNDIDILSSLPNTLLCHIMSFLPTKTSVWTMSLVSRRWRHLWKDLQVFQFYCYRLHSIPFKKFLLFVNTVLHLRSFRNIHKLHLDLTFHRSVFDNSYLNFVHCVESWILDAIGPQLQHLHLYIHIDAVIHLPPSLFINCSNLLSLSLSGKISMEVKHSSLHFPSLKMLSLGTEIMDESKLLSFLSGCPKLETLQVYFYFQPAVFVTNALVPSSSQSSKPANHNFTWTYFDINEGYINLGIVGNFHTMEEAFLNVFYRFESEFVDPVLDHLLVPHDAINLRLRHSTSKCSLHAPVLNYPEFRDLQNLKFILPCFNSNLLVHMLEKCRMLQVLIIQSNKEEPPPLRTWTPESTTVSECLKSHLTYVHIEGYQGLEDELTFAEYILQNGLVLRTMFIFVDSLMDKTDKECSLKRLTNISKGSVTCQLKFDPTVSS