AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25448

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25448.t1 MTR_8g070950 48.791 455 135 11 1 374 1 438 2.12e-119 353
MS.gene25448.t1 MTR_8g062010 48.741 437 129 9 22 374 9 434 1.05e-115 343
MS.gene25448.t1 MTR_8g062310 45.928 442 140 8 6 370 9 428 2.77e-113 337
MS.gene25448.t1 MTR_8g062300 64.202 257 64 3 136 375 237 482 6.98e-105 317
MS.gene25448.t1 MTR_8g062300 42.857 168 65 6 4 144 3 166 7.80e-23 100
MS.gene25448.t1 MTR_2g086020 55.937 320 114 6 57 374 25 319 3.76e-103 307
MS.gene25448.t1 MTR_8g062080 60.684 234 76 3 149 375 226 450 2.89e-88 273
MS.gene25448.t1 MTR_8g062080 42.564 195 82 7 1 171 1 189 1.37e-27 113
MS.gene25448.t1 MTR_8g469020 55.411 231 80 2 155 374 237 455 1.91e-82 259
MS.gene25448.t1 MTR_8g469020 39.869 153 53 6 22 145 7 149 3.57e-19 89.0
MS.gene25448.t1 MTR_8g058965 53.878 245 91 2 142 375 228 461 2.38e-78 248
MS.gene25448.t1 MTR_8g467790 36.250 400 126 12 21 330 147 507 1.48e-55 190
MS.gene25448.t1 MTR_4g050250 51.813 193 65 5 142 317 240 421 1.60e-54 185
MS.gene25448.t1 MTR_4g050250 37.059 170 64 6 9 145 8 167 1.95e-19 89.7
MS.gene25448.t1 MTR_8g077460 33.846 390 193 16 22 370 10 375 7.91e-42 150
MS.gene25448.t1 MTR_8g014260 37.063 286 136 10 115 370 180 451 4.10e-41 150
MS.gene25448.t1 MTR_4g024710 33.798 287 144 9 115 370 76 347 3.62e-34 129
MS.gene25448.t1 MTR_2g083840 40.741 216 110 5 159 370 256 457 4.79e-34 131
MS.gene25448.t1 MTR_2g083840 31.928 166 79 4 6 145 7 164 3.67e-13 70.9
MS.gene25448.t1 MTR_8g062270 37.681 207 104 6 171 369 264 453 5.38e-33 128
MS.gene25448.t1 MTR_8g468810 35.610 205 113 5 173 370 271 463 3.74e-30 120
MS.gene25448.t1 MTR_8g014210 39.086 197 104 6 175 370 50 231 7.57e-30 115
MS.gene25448.t1 MTR_8g058970 32.931 331 186 13 47 370 176 477 2.85e-28 115
MS.gene25448.t1 MTR_5g092960 36.058 208 108 4 173 369 268 461 9.84e-28 114
MS.gene25448.t1 MTR_5g092960 36.875 160 61 6 21 150 18 167 1.56e-13 72.0
MS.gene25448.t1 MTR_3g099800 62.069 87 32 1 282 367 19 105 1.09e-27 105
MS.gene25448.t1 MTR_8g467800 34.951 206 113 4 172 370 262 453 2.01e-27 112
MS.gene25448.t1 MTR_8g467800 36.879 141 62 5 6 126 2 135 4.13e-13 70.9
MS.gene25448.t1 MTR_4g032730 33.333 219 110 3 174 369 261 466 6.22e-26 108
MS.gene25448.t1 MTR_8g468840 35.644 202 112 4 171 368 261 448 1.90e-25 107
MS.gene25448.t1 MTR_8g445780 29.343 426 162 18 1 323 1 390 1.57e-23 101
MS.gene25448.t1 MTR_4g024745 31.111 270 140 9 115 353 30 284 8.13e-23 97.4
MS.gene25448.t1 MTR_7g098510 27.073 410 240 15 1 370 1 391 6.12e-21 93.6
MS.gene25448.t1 MTR_8g014160 53.684 95 38 3 278 370 335 425 6.55e-20 90.9
MS.gene25448.t1 MTR_7g032380 27.249 389 204 17 20 370 9 356 4.25e-18 85.1
MS.gene25448.t1 MTR_8g468760 36.196 163 87 6 216 369 264 418 1.70e-17 84.0
MS.gene25448.t1 MTR_7g098480 25.926 405 247 8 1 369 1 388 1.82e-16 80.9
MS.gene25448.t1 MTR_8g014230 41.667 84 47 2 280 363 17 98 2.62e-16 74.3
MS.gene25448.t1 MTR_7g098570 25.971 412 235 17 1 366 2 389 1.13e-15 78.6
MS.gene25448.t1 MTR_7g034115 27.154 383 223 17 20 362 9 375 6.32e-15 75.9
MS.gene25448.t1 MTR_5g021550 36.257 171 73 7 1 144 1 162 1.43e-14 71.2
MS.gene25448.t1 MTR_5g092960 36.943 157 65 5 21 150 18 167 1.83e-13 71.6
MS.gene25448.t1 MTR_8g467800 36.879 141 62 5 6 126 2 135 2.82e-13 70.5
MS.gene25448.t1 MTR_5g008950 25.127 394 219 17 24 370 1 365 3.39e-13 70.5
MS.gene25448.t1 MTR_1g041245 34.058 138 81 4 241 370 251 386 2.27e-12 68.2
MS.gene25448.t1 MTR_8g014290 33.537 164 70 6 15 145 3 160 1.79e-11 62.4
MS.gene25448.t1 MTR_7g032370 29.927 137 88 3 241 370 217 352 4.27e-11 63.9
MS.gene25448.t1 MTR_5g084070 30.102 196 114 6 173 362 241 419 4.65e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 76 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCGGAACGGACTTGTTT+TGG 0.289001 8.2:+40983327 MS.gene25448:CDS
CAACTTTATCTCAATGTTGA+TGG 0.302931 8.2:+40981927 MS.gene25448:CDS
TGATGTAAATCGCGAAATTC+AGG 0.303964 8.2:-40982948 None:intergenic
GAAATTCACTTCTGGTATCT+TGG 0.314508 8.2:-40982732 None:intergenic
CGTTGGTAACTTTCGTACTA+TGG 0.347099 8.2:+40982642 MS.gene25448:CDS
TCATCTCTGGAAGGATCTTC+AGG 0.350215 8.2:+40981695 MS.gene25448:CDS
ATGTTTCAACTCAAATCTTC+TGG 0.358687 8.2:+40982989 MS.gene25448:CDS
AGTTACTTTAATATAAATAA+AGG 0.368761 8.2:+40982601 MS.gene25448:CDS
GACCCAGCTGTATCTTCTTA+AGG 0.370771 8.2:+40983460 MS.gene25448:CDS
GCTTCTATGAAATTCACTTC+TGG 0.378368 8.2:-40982740 None:intergenic
TTTGAATGTCGCGGGATTTG+CGG 0.402806 8.2:-40981791 None:intergenic
CATCCTCATAGTTAATTATG+AGG 0.414466 8.2:-40982762 None:intergenic
CTTGGATGAGGTTGAGAATA+GGG 0.417120 8.2:-40982714 None:intergenic
ATATCCTTCTATGTGAATAT+AGG 0.424387 8.2:-40983266 None:intergenic
TGGGGAGGAAGGACATGATA+TGG 0.433088 8.2:-40981628 None:intergenic
CTGACCTATATTCACATAGA+AGG 0.433553 8.2:+40983262 MS.gene25448:CDS
TCTTGGATGAGGTTGAGAAT+AGG 0.442368 8.2:-40982715 None:intergenic
CTCGCAGGTATCTTCATCTC+TGG 0.455203 8.2:+40981682 MS.gene25448:CDS
AATTCAGGATAATTTAGAAC+AGG 0.463615 8.2:-40982933 None:intergenic
GAGATAAAGTTGTTGAAGGT+GGG 0.465366 8.2:-40981916 None:intergenic
CACTTCTGGTATCTTGGATG+AGG 0.469527 8.2:-40982726 None:intergenic
GCCTTTGATCGCCTTCAAAA+CGG 0.470214 8.2:+40981831 MS.gene25448:CDS
TCACCTTAAGAAGATACAGC+TGG 0.488366 8.2:-40983463 None:intergenic
TGAGATAAAGTTGTTGAAGG+TGG 0.491370 8.2:-40981917 None:intergenic
GAAGGATATCAAGGACTTGA+AGG 0.492911 8.2:+40983280 MS.gene25448:CDS
CATTGAGATAAAGTTGTTGA+AGG 0.494116 8.2:-40981920 None:intergenic
CTTCTATGTGAATATAGGTC+AGG 0.500493 8.2:-40983261 None:intergenic
GCACCAACCTCGTTTCCCTC+AGG 0.500754 8.2:+40981985 MS.gene25448:CDS
TACGATGAGCCTTCTCTCGC+AGG 0.508013 8.2:+40981667 MS.gene25448:CDS
CAGCTGGGTCGAATTTAAGT+TGG 0.510160 8.2:-40983447 None:intergenic
TCCGGCTGCCATGTTCTCAA+TGG 0.513864 8.2:-40983205 None:intergenic
TCTACAAATTCACTTTCAGC+CGG 0.514388 8.2:-40982691 None:intergenic
TTGTTGAAGGTGGGGTCCAA+TGG 0.515637 8.2:-40981907 None:intergenic
ATTCACATAGAAGGATATCA+AGG 0.519668 8.2:+40983271 MS.gene25448:CDS
ATTTATATTAAAGTAACTCC+AGG 0.524406 8.2:-40982596 None:intergenic
AAGCCTCATAATTAACTATG+AGG 0.525772 8.2:+40982759 MS.gene25448:CDS
AAGATTAACAAATGTATCGA+AGG 0.530416 8.2:+40983411 MS.gene25448:CDS
TTTAGAACAGGTGCATGAGT+TGG 0.547228 8.2:-40982921 None:intergenic
CGTCGATGGCGGAGGAGTCA+AGG 0.548687 8.2:+40981525 None:intergenic
AACAACCACCATTGAGAACA+TGG 0.552420 8.2:+40983197 MS.gene25448:CDS
CACCTTAAGAAGATACAGCT+GGG 0.554254 8.2:-40983462 None:intergenic
AGATAAAGTTGTTGAAGGTG+GGG 0.559902 8.2:-40981915 None:intergenic
ATGAAGATACCTGCGAGAGA+AGG 0.560446 8.2:-40981676 None:intergenic
GCGTGGATACTTGCTGCCAT+TGG 0.562356 8.2:+40981891 MS.gene25448:CDS
ACCATTGAGAACATGGCAGC+CGG 0.564288 8.2:+40983204 MS.gene25448:CDS
CCGCCGATGACAATTTCACC+TGG 0.564468 8.2:+40982578 MS.gene25448:CDS
TGAATCGACGTCGATGGCGG+AGG 0.566196 8.2:+40981517 None:intergenic
AATGCGACCTAGTCGTCGAC+AGG 0.567521 8.2:+40981576 MS.gene25448:CDS
ATATGGCATAACAACGAGTC+CGG 0.568277 8.2:-40981611 None:intergenic
CTACAAATTCACTTTCAGCC+GGG 0.571470 8.2:-40982690 None:intergenic
CGACAGGTTGAGCAGTCTAC+CGG 0.574240 8.2:+40981592 MS.gene25448:CDS
GAATGTCGCGGGATTTGCGG+AGG 0.575959 8.2:-40981788 None:intergenic
GGCTGCCATGTTCTCAATGG+TGG 0.577603 8.2:-40983202 None:intergenic
TGCTCAACCTGTCGACGACT+AGG 0.577840 8.2:-40981583 None:intergenic
CTATGTGAATATAGGTCAGG+TGG 0.580589 8.2:-40983258 None:intergenic
ATCGGCGGATATCAAGCTGT+TGG 0.583411 8.2:-40982563 None:intergenic
TGTGGCATTGCTCAAGACAA+TGG 0.586966 8.2:-40983013 None:intergenic
AGAAGATTTGAGTTGAAACA+TGG 0.586977 8.2:-40982987 None:intergenic
TGGTAACTTTCGTACTATGG+TGG 0.591391 8.2:+40982645 MS.gene25448:CDS
TCAGAAACTCTTGTTGACTC+CGG 0.591556 8.2:-40983223 None:intergenic
GATTTGAGTTGAAACATGGA+AGG 0.600986 8.2:-40982983 None:intergenic
GAGAAGGCTCATCGTAGTAA+CGG 0.611334 8.2:-40981660 None:intergenic
TTATGAGAACTTGAAGCATG+TGG 0.613522 8.2:-40983031 None:intergenic
CAGGTATCTTCATCTCTGGA+AGG 0.616499 8.2:+40981686 MS.gene25448:CDS
GTTGCATCATTCGATGTCAA+AGG 0.618438 8.2:+40982795 MS.gene25448:CDS
TTACCTGAGGGAAACGAGGT+TGG 0.622908 8.2:-40981988 None:intergenic
AGATTAACAAATGTATCGAA+GGG 0.622978 8.2:+40983412 MS.gene25448:CDS
AACTCAAGAATGCTGAAGCG+TGG 0.623379 8.2:+40981874 MS.gene25448:CDS
ACTCCAGGTGAAATTGTCAT+CGG 0.626403 8.2:-40982581 None:intergenic
GATCAAAGGCGAAAGTGAGA+TGG 0.632265 8.2:-40981818 None:intergenic
CAATTGATAAAGAGTGAACG+AGG 0.640528 8.2:-40981963 None:intergenic
TATGTGAATATAGGTCAGGT+GGG 0.652099 8.2:-40983257 None:intergenic
CCAGGTGAAATTGTCATCGG+CGG 0.657457 8.2:-40982578 None:intergenic
TCTCATAATTAAGAGCAACA+AGG 0.667425 8.2:+40983046 MS.gene25448:CDS
GAAGTTACCTGAGGGAAACG+AGG 0.701057 8.2:-40981992 None:intergenic
AATTGATAAAGAGTGAACGA+GGG 0.746792 8.2:-40981962 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTACTTTAATATAAATAA+AGG + chr8.2:40982601-40982620 MS.gene25448:CDS 10.0%
!! AAAAAGAAGAAGAAATAAAT+TGG + chr8.2:40982373-40982392 MS.gene25448:intron 15.0%
!! AAAATATATTTGTGTACAAA+TGG - chr8.2:40983139-40983158 None:intergenic 15.0%
!! AATTTAATTCAAACATTCTT+TGG + chr8.2:40983084-40983103 MS.gene25448:intron 15.0%
!! ATTTATATTAAAGTAACTCC+AGG - chr8.2:40982599-40982618 None:intergenic 20.0%
!!! AAATAAAGGACGTTTTAAAT+TGG + chr8.2:40982615-40982634 MS.gene25448:CDS 20.0%
!!! AATAAAGGACGTTTTAAATT+GGG + chr8.2:40982616-40982635 MS.gene25448:CDS 20.0%
!!! ACAAATATATTTTTGAGTCA+CGG + chr8.2:40983146-40983165 MS.gene25448:intron 20.0%
!!! ATGTTTTTAAATGTGTAGTT+TGG + chr8.2:40982842-40982861 MS.gene25448:intron 20.0%
!!! TGTTTTTGTTGATATTTCAA+TGG + chr8.2:40983360-40983379 MS.gene25448:CDS 20.0%
!!! TTGTTAATCTTTTGATAGAA+CGG - chr8.2:40983402-40983421 None:intergenic 20.0%
! AAGATTAACAAATGTATCGA+AGG + chr8.2:40983411-40983430 MS.gene25448:CDS 25.0%
! AGATTAACAAATGTATCGAA+GGG + chr8.2:40983412-40983431 MS.gene25448:CDS 25.0%
! ATATCCTTCTATGTGAATAT+AGG - chr8.2:40983269-40983288 None:intergenic 25.0%
! CATGACCAAAAAATTTGATT+TGG - chr8.2:40982420-40982439 None:intergenic 25.0%
! TAATTCAAACATTCTTTGGT+CGG + chr8.2:40983088-40983107 MS.gene25448:intron 25.0%
!! AATTCAGGATAATTTAGAAC+AGG - chr8.2:40982936-40982955 None:intergenic 25.0%
!!! AAGGTGAAGTTTTACTTTTA+TGG + chr8.2:40982814-40982833 MS.gene25448:intron 25.0%
!!! AGTGACCAAATCAAATTTTT+TGG + chr8.2:40982412-40982431 MS.gene25448:intron 25.0%
!!! ATTTCTTGATGTTTTTTTCC+CGG + chr8.2:40982672-40982691 MS.gene25448:CDS 25.0%
!!! CATTTGCTGAATATATTTTG+CGG + chr8.2:40983311-40983330 MS.gene25448:CDS 25.0%
AAGCCTCATAATTAACTATG+AGG + chr8.2:40982759-40982778 MS.gene25448:CDS 30.0%
AATTGATAAAGAGTGAACGA+GGG - chr8.2:40981965-40981984 None:intergenic 30.0%
ATGTTTCAACTCAAATCTTC+TGG + chr8.2:40982989-40983008 MS.gene25448:CDS 30.0%
ATTCACATAGAAGGATATCA+AGG + chr8.2:40983271-40983290 MS.gene25448:CDS 30.0%
CAACTTTATCTCAATGTTGA+TGG + chr8.2:40981927-40981946 MS.gene25448:CDS 30.0%
CATCCTCATAGTTAATTATG+AGG - chr8.2:40982765-40982784 None:intergenic 30.0%
CATTGAGATAAAGTTGTTGA+AGG - chr8.2:40981923-40981942 None:intergenic 30.0%
GAAATAAATTGGCAATGCTT+TGG + chr8.2:40982384-40982403 MS.gene25448:intron 30.0%
TAAAAACAGAAGTTACCTGA+GGG - chr8.2:40982003-40982022 None:intergenic 30.0%
TCTCATAATTAAGAGCAACA+AGG + chr8.2:40983046-40983065 MS.gene25448:CDS 30.0%
TGTGACCAAATTCATATTCT+TGG - chr8.2:40982302-40982321 None:intergenic 30.0%
TTAAAAACAGAAGTTACCTG+AGG - chr8.2:40982004-40982023 None:intergenic 30.0%
TTACTAATGACGAAACTGTT+TGG - chr8.2:40981755-40981774 None:intergenic 30.0%
TTCAAAAGTGAAAGTGAGTT+TGG - chr8.2:40982099-40982118 None:intergenic 30.0%
TTGGACCAAGAATATGAATT+TGG + chr8.2:40982294-40982313 MS.gene25448:intron 30.0%
! CTTCAGTGATGGAAAATAAA+CGG - chr8.2:40982195-40982214 None:intergenic 30.0%
! TTTTAATCCCATCTAATTGC+AGG + chr8.2:40982030-40982049 MS.gene25448:intron 30.0%
!! AGAAGATTTGAGTTGAAACA+TGG - chr8.2:40982990-40983009 None:intergenic 30.0%
AATGACGAAACTGTTTGGAA+CGG - chr8.2:40981750-40981769 None:intergenic 35.0%
AGATAAAGTTGTTGAAGGTG+GGG - chr8.2:40981918-40981937 None:intergenic 35.0%
ATGAGTATCCTGCAATTAGA+TGG - chr8.2:40982041-40982060 None:intergenic 35.0%
CAATTGATAAAGAGTGAACG+AGG - chr8.2:40981966-40981985 None:intergenic 35.0%
CTGACCTATATTCACATAGA+AGG + chr8.2:40983262-40983281 MS.gene25448:CDS 35.0%
CTTCTATGTGAATATAGGTC+AGG - chr8.2:40983264-40983283 None:intergenic 35.0%
GAAATTCACTTCTGGTATCT+TGG - chr8.2:40982735-40982754 None:intergenic 35.0%
GAGATAAAGTTGTTGAAGGT+GGG - chr8.2:40981919-40981938 None:intergenic 35.0%
GCTTCTATGAAATTCACTTC+TGG - chr8.2:40982743-40982762 None:intergenic 35.0%
TATGTGAATATAGGTCAGGT+GGG - chr8.2:40983260-40983279 None:intergenic 35.0%
TCTACAAATTCACTTTCAGC+CGG - chr8.2:40982694-40982713 None:intergenic 35.0%
TGAGATAAAGTTGTTGAAGG+TGG - chr8.2:40981920-40981939 None:intergenic 35.0%
TGAGTATCCTGCAATTAGAT+GGG - chr8.2:40982040-40982059 None:intergenic 35.0%
TGATGTAAATCGCGAAATTC+AGG - chr8.2:40982951-40982970 None:intergenic 35.0%
TTAAGAGCAACAAGGTTTGT+TGG + chr8.2:40983054-40983073 MS.gene25448:intron 35.0%
TTATGAGAACTTGAAGCATG+TGG - chr8.2:40983034-40983053 None:intergenic 35.0%
TTGTTATCATGTATGTGCAG+TGG + chr8.2:40982898-40982917 MS.gene25448:intron 35.0%
TTTCTCTCTCTCATTGTTGT+GGG - chr8.2:40982344-40982363 None:intergenic 35.0%
! GCTGAATATATTTTGCGGAA+CGG + chr8.2:40983316-40983335 MS.gene25448:CDS 35.0%
! TTTTCTCTCTCTCATTGTTG+TGG - chr8.2:40982345-40982364 None:intergenic 35.0%
!! GATTTGAGTTGAAACATGGA+AGG - chr8.2:40982986-40983005 None:intergenic 35.0%
!! GTAGTAACGGAAGTTTTTGT+GGG - chr8.2:40981650-40981669 None:intergenic 35.0%
!! TAGTAACGGAAGTTTTTGTG+GGG - chr8.2:40981649-40981668 None:intergenic 35.0%
!!! TATATTTTTGAGTCACGGCT+TGG + chr8.2:40983151-40983170 MS.gene25448:intron 35.0%
AACAACCACCATTGAGAACA+TGG + chr8.2:40983197-40983216 MS.gene25448:CDS 40.0%
ACTCCAGGTGAAATTGTCAT+CGG - chr8.2:40982584-40982603 None:intergenic 40.0%
AGAGACAACATCTTCAGTGA+TGG - chr8.2:40982206-40982225 None:intergenic 40.0%
ATATGGCATAACAACGAGTC+CGG - chr8.2:40981614-40981633 None:intergenic 40.0%
CTACAAATTCACTTTCAGCC+GGG - chr8.2:40982693-40982712 None:intergenic 40.0%
CTATGTGAATATAGGTCAGG+TGG - chr8.2:40983261-40983280 None:intergenic 40.0%
CTTGGATGAGGTTGAGAATA+GGG - chr8.2:40982717-40982736 None:intergenic 40.0%
GAAGGATATCAAGGACTTGA+AGG + chr8.2:40983280-40983299 MS.gene25448:CDS 40.0%
GTTGCATCATTCGATGTCAA+AGG + chr8.2:40982795-40982814 MS.gene25448:CDS 40.0%
TCACTGAAGATGTTGTCTCT+CGG + chr8.2:40982206-40982225 MS.gene25448:intron 40.0%
TCTTGGATGAGGTTGAGAAT+AGG - chr8.2:40982718-40982737 None:intergenic 40.0%
TGGTAACTTTCGTACTATGG+TGG + chr8.2:40982645-40982664 MS.gene25448:CDS 40.0%
TTCTAATACCGCAGTCTTCA+CGG + chr8.2:40982128-40982147 MS.gene25448:intron 40.0%
TTCTCTCTCTCATTGTTGTG+GGG - chr8.2:40982343-40982362 None:intergenic 40.0%
! CAAGAACACAACCGTTTTGA+AGG - chr8.2:40981845-40981864 None:intergenic 40.0%
! TTTAGAACAGGTGCATGAGT+TGG - chr8.2:40982924-40982943 None:intergenic 40.0%
!! CGTAGTAACGGAAGTTTTTG+TGG - chr8.2:40981651-40981670 None:intergenic 40.0%
!! CGTTGGTAACTTTCGTACTA+TGG + chr8.2:40982642-40982661 MS.gene25448:CDS 40.0%
!! CGTTTTAAATTGGGCATCGT+TGG + chr8.2:40982625-40982644 MS.gene25448:CDS 40.0%
!! TCAGAAACTCTTGTTGACTC+CGG - chr8.2:40983226-40983245 None:intergenic 40.0%
!! TTAGTTTTCTTTCTCGCTGG+CGG + chr8.2:40982254-40982273 MS.gene25448:intron 40.0%
!!! ATGGAAGTTTTGAATGTCGC+GGG - chr8.2:40981802-40981821 None:intergenic 40.0%
!!! GATGGAAGTTTTGAATGTCG+CGG - chr8.2:40981803-40981822 None:intergenic 40.0%
!!! GCATTAGTTTTCTTTCTCGC+TGG + chr8.2:40982251-40982270 MS.gene25448:intron 40.0%
AACTCAAGAATGCTGAAGCG+TGG + chr8.2:40981874-40981893 MS.gene25448:CDS 45.0%
ACATGTCGTCATCGGAAGAA+AGG - chr8.2:40982507-40982526 None:intergenic 45.0%
ATGAAGATACCTGCGAGAGA+AGG - chr8.2:40981679-40981698 None:intergenic 45.0%
CACTTCTGGTATCTTGGATG+AGG - chr8.2:40982729-40982748 None:intergenic 45.0%
CAGCTGGGTCGAATTTAAGT+TGG - chr8.2:40983450-40983469 None:intergenic 45.0%
CAGGTATCTTCATCTCTGGA+AGG + chr8.2:40981686-40981705 MS.gene25448:CDS 45.0%
GAGAAGGCTCATCGTAGTAA+CGG - chr8.2:40981663-40981682 None:intergenic 45.0%
GATCAAAGGCGAAAGTGAGA+TGG - chr8.2:40981821-40981840 None:intergenic 45.0%
GCCTTTGATCGCCTTCAAAA+CGG + chr8.2:40981831-40981850 MS.gene25448:CDS 45.0%
GGAAAATTCTTGGGTGGTCT+TGG + chr8.2:40982275-40982294 MS.gene25448:intron 45.0%
GGTCAAAGACATGTCGTCAT+CGG - chr8.2:40982515-40982534 None:intergenic 45.0%
TGTGGCATTGCTCAAGACAA+TGG - chr8.2:40983016-40983035 None:intergenic 45.0%
TTTGAATGTCGCGGGATTTG+CGG - chr8.2:40981794-40981813 None:intergenic 45.0%
! TAACGGAAGTTTTTGTGGGG+AGG - chr8.2:40981646-40981665 None:intergenic 45.0%
! TCATCTCTGGAAGGATCTTC+AGG + chr8.2:40981695-40981714 MS.gene25448:CDS 45.0%
!! GGAAGGTGTCACTAGAACTT+TGG - chr8.2:40982536-40982555 None:intergenic 45.0%
!! GTGGGGTGTCATTCATTGAT+TGG - chr8.2:40982326-40982345 None:intergenic 45.0%
!! TTTGCGGAACGGACTTGTTT+TGG + chr8.2:40983327-40983346 MS.gene25448:CDS 45.0%
!!! ACCGTTTTGAAGGCGATCAA+AGG - chr8.2:40981835-40981854 None:intergenic 45.0%
ACCATTGAGAACATGGCAGC+CGG + chr8.2:40983204-40983223 MS.gene25448:CDS 50.0%
ACCTAAGACTATCTCCTCGC+TGG + chr8.2:40982445-40982464 MS.gene25448:intron 50.0%
ATCGGCGGATATCAAGCTGT+TGG - chr8.2:40982566-40982585 None:intergenic 50.0%
CCAGGTGAAATTGTCATCGG+CGG - chr8.2:40982581-40982600 None:intergenic 50.0%
CGATTGAACCGTGAAGACTG+CGG - chr8.2:40982139-40982158 None:intergenic 50.0%
CGGCAATGATATCGTGGATC+AGG + chr8.2:40982226-40982245 MS.gene25448:intron 50.0%
CTCGCAGGTATCTTCATCTC+TGG + chr8.2:40981682-40981701 MS.gene25448:CDS 50.0%
CTCGCTGGCGGAAAATTCTT+GGG + chr8.2:40982266-40982285 MS.gene25448:intron 50.0%
GAAGTTACCTGAGGGAAACG+AGG - chr8.2:40981995-40982014 None:intergenic 50.0%
GGATATCAAGCTGTTGGAGC+TGG - chr8.2:40982560-40982579 None:intergenic 50.0%
GTCTCTCGGCAATGATATCG+TGG + chr8.2:40982220-40982239 MS.gene25448:intron 50.0%
GTTCCTCCTACGAGCAACAA+GGG - chr8.2:40983180-40983199 None:intergenic 50.0%
TATCAAGCTGTTGGAGCTGG+AGG - chr8.2:40982557-40982576 None:intergenic 50.0%
TCTCGCTGGCGGAAAATTCT+TGG + chr8.2:40982265-40982284 MS.gene25448:intron 50.0%
TGGGGAGGAAGGACATGATA+TGG - chr8.2:40981631-40981650 None:intergenic 50.0%
TGTTCCTCCTACGAGCAACA+AGG - chr8.2:40983181-40983200 None:intergenic 50.0%
TTACCTGAGGGAAACGAGGT+TGG - chr8.2:40981991-40982010 None:intergenic 50.0%
TTGTTGAAGGTGGGGTCCAA+TGG - chr8.2:40981910-40981929 None:intergenic 50.0%
! AGCGAGGAGATAGTCTTAGG+TGG - chr8.2:40982446-40982465 None:intergenic 50.0%
! GGAAGTTTTTGTGGGGAGGA+AGG - chr8.2:40981642-40981661 None:intergenic 50.0%
AACCTGCAAAGTCTCCAGCC+TGG - chr8.2:40982476-40982495 None:intergenic 55.0%
AAGCTGTTGGAGCTGGAGGA+AGG - chr8.2:40982553-40982572 None:intergenic 55.0%
AATGCGACCTAGTCGTCGAC+AGG + chr8.2:40981576-40981595 MS.gene25448:CDS 55.0%
ACCTGCAAAGTCTCCAGCCT+GGG - chr8.2:40982475-40982494 None:intergenic 55.0%
CCGCCGATGACAATTTCACC+TGG + chr8.2:40982578-40982597 MS.gene25448:CDS 55.0%
GCCAGCGAGGAGATAGTCTT+AGG - chr8.2:40982449-40982468 None:intergenic 55.0%
GCTGGCGGAAAATTCTTGGG+TGG + chr8.2:40982269-40982288 MS.gene25448:intron 55.0%
GGCTGCCATGTTCTCAATGG+TGG - chr8.2:40983205-40983224 None:intergenic 55.0%
TACGATGAGCCTTCTCTCGC+AGG + chr8.2:40981667-40981686 MS.gene25448:CDS 55.0%
TCCGGCTGCCATGTTCTCAA+TGG - chr8.2:40983208-40983227 None:intergenic 55.0%
TGCTCAACCTGTCGACGACT+AGG - chr8.2:40981586-40981605 None:intergenic 55.0%
TGGTGTCCCTTGTTGCTCGT+AGG + chr8.2:40983171-40983190 MS.gene25448:intron 55.0%
TGTCCCTTGTTGCTCGTAGG+AGG + chr8.2:40983174-40983193 MS.gene25448:intron 55.0%
! CGACAGGTTGAGCAGTCTAC+CGG + chr8.2:40981592-40981611 MS.gene25448:CDS 55.0%
! GCGTGGATACTTGCTGCCAT+TGG + chr8.2:40981891-40981910 MS.gene25448:CDS 55.0%
CCTGCAAAGTCTCCAGCCTG+GGG - chr8.2:40982474-40982493 None:intergenic 60.0%
GAATGTCGCGGGATTTGCGG+AGG - chr8.2:40981791-40981810 None:intergenic 60.0%
GCACCAACCTCGTTTCCCTC+AGG + chr8.2:40981985-40982004 MS.gene25448:CDS 60.0%
CCCCAGGCTGGAGACTTTGC+AGG + chr8.2:40982471-40982490 MS.gene25448:intron 65.0%
! ATCTCCTCGCTGGCTGCCCC+AGG + chr8.2:40982455-40982474 MS.gene25448:intron 70.0%
! CCAGCCTGGGGCAGCCAGCG+AGG - chr8.2:40982462-40982481 None:intergenic 80.0%
!! CCTCGCTGGCTGCCCCAGGC+TGG + chr8.2:40982459-40982478 MS.gene25448:intron 80.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 40981530 40983480 40981530 ID=MS.gene25448
chr8.2 mRNA 40981530 40983480 40981530 ID=MS.gene25448.t1;Parent=MS.gene25448
chr8.2 exon 40981530 40981716 40981530 ID=MS.gene25448.t1.exon1;Parent=MS.gene25448.t1
chr8.2 CDS 40981530 40981716 40981530 ID=cds.MS.gene25448.t1;Parent=MS.gene25448.t1
chr8.2 exon 40981760 40982006 40981760 ID=MS.gene25448.t1.exon2;Parent=MS.gene25448.t1
chr8.2 CDS 40981760 40982006 40981760 ID=cds.MS.gene25448.t1;Parent=MS.gene25448.t1
chr8.2 exon 40982561 40982816 40982561 ID=MS.gene25448.t1.exon3;Parent=MS.gene25448.t1
chr8.2 CDS 40982561 40982816 40982561 ID=cds.MS.gene25448.t1;Parent=MS.gene25448.t1
chr8.2 exon 40982918 40983067 40982918 ID=MS.gene25448.t1.exon4;Parent=MS.gene25448.t1
chr8.2 CDS 40982918 40983067 40982918 ID=cds.MS.gene25448.t1;Parent=MS.gene25448.t1
chr8.2 exon 40983193 40983480 40983193 ID=MS.gene25448.t1.exon5;Parent=MS.gene25448.t1
chr8.2 CDS 40983193 40983480 40983193 ID=cds.MS.gene25448.t1;Parent=MS.gene25448.t1
Gene Sequence

>MS.gene25448

ATGGCGGAGGAGTCAAGGATGATGAAACGACTGAAAGCACAAGAAGAATGCGACCTAGTCGTCGACAGGTTGAGCAGTCTACCGGACTCGTTGTTATGCCATATCATGTCCTTCCTCCCCACAAAAACTTCCGTTACTACGATGAGCCTTCTCTCGCAGGTATCTTCATCTCTGGAAGGATCTTCAGGTCTTGTATTTCGACTTTCACGACTCAATTCCGTTCCAAACAGTTTCGTCATTAGTAAACTCTGTTCTCGTCCTCCGCAAATCCCGCGACATTCAAAACTTCCATCTCACTTTCGCCTTTGATCGCCTTCAAAACGGTTGTGTTCTTGAAAAGTTACAACTCAAGAATGCTGAAGCGTGGATACTTGCTGCCATTGGACCCCACCTTCAACAACTTTATCTCAATGTTGATGGTTGTACAGATATCCCTCGTTCACTCTTTATCAATTGCACCAACCTCGTTTCCCTCAGGTAACTTCTGTTTTTAAATTAATTTTTAATCCCATCTAATTGCAGGATACTCATGTTTTAATGATAACTCAAGTGATTTGTTTTTATGTCCAAACTCACTTTCACTTTTGAATCATACTTTTTCTAATACCGCAGTCTTCACGGTTCAATCGAAACTATGCACTGCAATATTAACAGTGCAAATTCCGTTTATTTTCCATCACTGAAGATGTTGTCTCTCGGCAATGATATCGTGGATCAGGAAGCATTAGTTTTCTTTCTCGCTGGCGGAAAATTCTTGGGTGGTCTTGGACCAAGAATATGAATTTGGTCACATCCAATCAATGAATGACACCCCACAACAATGAGAGAGAGAAAAAAAACTAAAAAAAGAAGAAGAAATAAATTGGCAATGCTTTGGAGTCTAGTGACCAAATCAAATTTTTTGGTCATGTGACCACCTAAGACTATCTCCTCGCTGGCTGCCCCAGGCTGGAGACTTTGCAGGTTTACGTTTACCTTTCTTCCGATGACGACATGTCTTTGACCAAAGTTCTAGTGACACCTTCCTCCAGCTCCAACAGCTTGATATCCGCCGATGACAATTTCACCTGGAGTTACTTTAATATAAATAAAGGACGTTTTAAATTGGGCATCGTTGGTAACTTTCGTACTATGGTGGAAGCATTTCTTGATGTTTTTTTCCCGGCTGAAAGTGAATTTGTAGACCCTATTCTCAACCTCATCCAAGATACCAGAAGTGAATTTCATAGAAGCCTCATAATTAACTATGAGGATGTTTATCTACAGTTGCATCATTCGATGTCAAAGGTGAAGTTTTACTTTTATGGTTTACATGTTTTTAAATGTGTAGTTTGGTTCTAAATTCTAATATTGCGTTGTCATCACTTGTTGTTATCATGTATGTGCAGTGGCCAACTCATGCACCTGTTCTAAATTATCCTGAATTTCGCGATTTACATCATTTGAAGTTCATCCTTCCATGTTTCAACTCAAATCTTCTGGTCCATTGTCTTGAGCAATGCCACATGCTTCAAGTTCTCATAATTAAGAGCAACAAGGTTTGTTGGATTTTCAAATTTAATTCAAACATTCTTTGGTCGGTCTATATATACATGCATGTATGTCTCCATTTGTACACAAATATATTTTTGAGTCACGGCTTGGTGTCCCTTGTTGCTCGTAGGAGGAACAACCACCATTGAGAACATGGCAGCCGGAGTCAACAAGAGTTTCTGAATGTCTCAAGTCCCACCTGACCTATATTCACATAGAAGGATATCAAGGACTTGAAGGTGAGCTGACATTTGCTGAATATATTTTGCGGAACGGACTTGTTTTGGAGACAATGCTTGTTTTTGTTGATATTTCAATGGACATAACGAACAAGTACCGTTCTATCAAAAGATTAACAAATGTATCGAAGGGCTCTGTCAAATGCCAACTTAAATTCGACCCAGCTGTATCTTCTTAA
Protein sequence

>MS.gene25448.t1

MAEESRMMKRLKAQEECDLVVDRLSSLPDSLLCHIMSFLPTKTSVTTMSLLSQVSSSLEGSSVSSLVNSVLVLRKSRDIQNFHLTFAFDRLQNGCVLEKLQLKNAEAWILAAIGPHLQQLYLNVDGCTDIPRSLFINCTNLVSLSSNSLISADDNFTWSYFNINKGRFKLGIVGNFRTMVEAFLDVFFPAESEFVDPILNLIQDTRSEFHRSLIINYEDVYLQLHHSMSKWPTHAPVLNYPEFRDLHHLKFILPCFNSNLLVHCLEQCHMLQVLIIKSNKEEQPPLRTWQPESTRVSECLKSHLTYIHIEGYQGLEGELTFAEYILRNGLVLETMLVFVDISMDITNKYRSIKRLTNVSKGSVKCQLKFDPAVSS