AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25449

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25449.t1 MTR_8g070950 64.938 405 125 9 1 394 41 439 4.78e-165 470
MS.gene25449.t1 MTR_8g062010 56.079 403 164 7 1 393 35 434 9.41e-131 382
MS.gene25449.t1 MTR_8g062310 55.076 394 160 10 4 394 54 433 1.41e-129 379
MS.gene25449.t1 MTR_8g062300 51.963 433 165 9 1 393 52 481 3.89e-128 377
MS.gene25449.t1 MTR_8g469020 51.395 430 165 12 2 394 34 456 2.00e-127 375
MS.gene25449.t1 MTR_8g062080 53.027 413 162 13 1 393 49 449 6.15e-127 373
MS.gene25449.t1 MTR_8g058965 51.628 430 161 12 2 393 40 460 3.15e-119 354
MS.gene25449.t1 MTR_8g467790 53.406 367 111 12 1 343 174 504 8.29e-107 324
MS.gene25449.t1 MTR_4g050250 47.644 382 145 13 1 335 47 420 2.73e-92 284
MS.gene25449.t1 MTR_2g086020 73.288 146 38 1 250 394 175 320 2.90e-65 211
MS.gene25449.t1 MTR_2g086020 44.604 139 67 5 1 131 1 137 6.51e-22 95.9
MS.gene25449.t1 MTR_2g083840 38.849 417 217 15 3 389 49 457 5.57e-61 204
MS.gene25449.t1 MTR_8g014260 38.369 417 217 15 4 389 44 451 2.09e-59 199
MS.gene25449.t1 MTR_8g062270 35.613 424 226 12 1 388 41 453 3.84e-56 191
MS.gene25449.t1 MTR_8g468810 37.939 427 213 20 1 389 51 463 2.63e-54 186
MS.gene25449.t1 MTR_8g467800 36.879 423 227 15 2 394 46 458 4.09e-53 183
MS.gene25449.t1 MTR_5g092960 35.349 430 221 17 3 389 47 462 1.11e-52 182
MS.gene25449.t1 MTR_8g468840 37.113 388 204 13 2 356 40 420 5.76e-51 177
MS.gene25449.t1 MTR_8g468760 35.613 424 198 14 1 388 34 418 3.23e-49 172
MS.gene25449.t1 MTR_8g058970 34.234 444 226 19 1 389 45 477 2.60e-48 171
MS.gene25449.t1 MTR_8g445780 36.986 365 189 17 4 342 41 390 2.41e-46 163
MS.gene25449.t1 MTR_4g032730 34.023 435 229 16 3 389 43 467 9.44e-45 161
MS.gene25449.t1 MTR_8g014160 31.068 412 207 16 2 389 67 425 7.77e-35 133
MS.gene25449.t1 MTR_8g468810 37.126 334 163 17 1 300 51 371 7.94e-35 132
MS.gene25449.t1 MTR_5g092960 34.821 336 170 13 3 300 47 371 7.92e-34 130
MS.gene25449.t1 MTR_4g024710 36.054 294 165 11 111 389 62 347 1.81e-33 128
MS.gene25449.t1 MTR_8g077460 45.455 143 75 3 248 389 235 375 1.43e-29 118
MS.gene25449.t1 MTR_8g468810 38.911 257 118 14 1 230 51 295 1.77e-28 114
MS.gene25449.t1 MTR_8g468810 38.911 257 118 14 1 230 51 295 2.52e-28 114
MS.gene25449.t1 MTR_8g468810 38.911 257 118 14 1 230 51 295 4.03e-28 113
MS.gene25449.t1 MTR_4g024745 35.401 274 154 11 109 367 14 279 3.07e-26 107
MS.gene25449.t1 MTR_8g014210 36.134 238 132 10 165 389 1 231 2.22e-25 103
MS.gene25449.t1 MTR_8g467800 36.471 255 133 12 2 234 46 293 1.38e-23 100
MS.gene25449.t1 MTR_3g099800 61.842 76 27 2 302 375 19 94 1.66e-22 91.7
MS.gene25449.t1 MTR_5g084070 25.316 395 262 12 2 376 39 420 1.08e-13 72.8
MS.gene25449.t1 MTR_8g014230 43.590 78 43 1 300 377 17 93 1.10e-13 67.0
MS.gene25449.t1 MTR_5g080710 28.571 231 155 4 138 361 198 425 4.59e-13 70.9
MS.gene25449.t1 MTR_8g014290 40.323 124 52 6 4 106 38 160 1.45e-12 65.5
MS.gene25449.t1 MTR_7g098510 24.570 407 229 17 2 389 44 391 2.05e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25449.t1 AT5G50270 27.363 402 239 15 3 374 30 408 1.31e-16 81.3
MS.gene25449.t1 AT5G56560 26.634 413 250 17 3 389 33 418 7.70e-15 76.3
MS.gene25449.t1 AT5G56560 28.475 295 182 10 111 389 326 607 5.60e-13 70.9
MS.gene25449.t1 AT2G26030 26.897 435 250 20 2 390 30 442 7.68e-13 70.1
MS.gene25449.t1 AT2G26030 26.897 435 250 20 2 390 30 442 7.68e-13 70.1
MS.gene25449.t1 AT4G10400 26.119 402 263 15 2 389 28 409 1.15e-12 69.3
MS.gene25449.t1 AT4G10400 26.119 402 263 15 2 389 28 409 1.15e-12 69.3
MS.gene25449.t1 AT4G10400 26.119 402 263 15 2 389 28 409 1.15e-12 69.3
MS.gene25449.t1 AT2G26030 26.879 439 250 21 2 390 30 447 2.21e-12 68.6
MS.gene25449.t1 AT5G38570 24.372 398 240 12 3 389 29 376 4.02e-12 67.8
MS.gene25449.t1 AT5G50270 26.506 249 152 9 3 229 30 269 8.38e-11 63.2

Find 83 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGGAAACTTTAATAATA+TGG 0.213057 8.2:-40987861 MS.gene25449:CDS
CACTTTCTGCCCCTGTTCTA+AGG 0.248751 8.2:-40987409 MS.gene25449:CDS
TTTGCGGAACGGGCTTGTTT+TGG 0.301181 8.2:-40987014 MS.gene25449:CDS
AGCACAGTGTTGACGAATAA+TGG 0.325293 8.2:+40988724 None:intergenic
GGGAAAACATGTTTAAGTTA+TGG 0.346779 8.2:-40988636 MS.gene25449:CDS
CTCGTAGGTGGCGCCACCTC+TGG 0.349675 8.2:-40988807 MS.gene25449:CDS
CGTGTCACATTGGGCATCAT+TGG 0.351168 8.2:-40987878 MS.gene25449:CDS
ATTGGTAGATTTCAATCTCT+TGG 0.357522 8.2:+40987943 None:intergenic
ATGTTTCAACTCAAATCTTC+TGG 0.358687 8.2:-40987342 MS.gene25449:CDS
AACCATGTTATATCTCCTTA+AGG 0.368169 8.2:-40986881 None:intergenic
AACTCTGGATACCTTAGAAC+AGG 0.387304 8.2:+40987398 None:intergenic
CTCGGATGTCTTTGAGAATA+GGG 0.388547 8.2:+40987789 None:intergenic
TTATGGATCCGTACTGCTAC+TGG 0.402373 8.2:-40988619 MS.gene25449:CDS
TCTCGGATGTCTTTGAGAAT+AGG 0.404973 8.2:+40987788 None:intergenic
ATTCATGTACAAGGATATCT+AGG 0.421162 8.2:-40987070 MS.gene25449:CDS
TCTCTCCGTCTGCCCCGTGC+TGG 0.428073 8.2:-40988041 MS.gene25449:CDS
GAGCTGGAGGAAGGTGGAAC+AGG 0.435822 8.2:+40987965 None:intergenic
CGATCTAATTTGCGAAACTC+TGG 0.442892 8.2:+40987383 None:intergenic
ACGGAGAGAAAGGAAACTAT+TGG 0.444858 8.2:+40988055 None:intergenic
AGATTATCTGAGATAACAAT+TGG 0.445912 8.2:-40986929 MS.gene25449:CDS
ATAGATCCCCGTGTCACATT+GGG 0.455209 8.2:-40987887 MS.gene25449:CDS
CACGGGGCAGACGGAGAGAA+AGG 0.456042 8.2:+40988045 None:intergenic
AAAGTAGAGAAATATCATAT+TGG 0.462773 8.2:+40987762 None:intergenic
CTACCAATCACAATTTCACT+TGG 0.468189 8.2:-40987928 MS.gene25449:CDS
CTGCCCCGTGCTGGAAACAC+TGG 0.475059 8.2:-40988032 MS.gene25449:CDS
AGATTTCAATCTCTTGGAGC+TGG 0.476427 8.2:+40987949 None:intergenic
CTTGTACATGAATATATGTC+AGG 0.499325 8.2:+40987080 None:intergenic
AGTCCAAGTGAAATTGTGAT+TGG 0.501196 8.2:+40987925 None:intergenic
CAATCCAGTGTTTCCAGCAC+GGG 0.503073 8.2:+40988028 None:intergenic
GAGATAGAGTTCTTCAAGGT+GGG 0.506876 8.2:+40988594 None:intergenic
TTTCTCGATGTCCTTTCCCC+AGG 0.512089 8.2:-40987830 MS.gene25449:CDS
CACCTTAAGGAGATATAACA+TGG 0.521086 8.2:+40986879 None:intergenic
CTTGTAGCTGCTGCCAGAGG+TGG 0.531131 8.2:+40988794 None:intergenic
AATCTCTTGGAGCTGGAGGA+AGG 0.531230 8.2:+40987956 None:intergenic
TGTTTCCAGCACGGGGCAGA+CGG 0.535232 8.2:+40988036 None:intergenic
TATAGATCCCCGTGTCACAT+TGG 0.538369 8.2:-40987888 MS.gene25449:CDS
GAATGTTGGGGAATCTGCGG+AGG 0.539616 8.2:+40988698 None:intergenic
AAATATCATATTGGTCATCT+CGG 0.543175 8.2:+40987771 None:intergenic
TGGAGAGATAGAGTTCTTCA+AGG 0.545549 8.2:+40988590 None:intergenic
AATGATGCCCAATGTGACAC+GGG 0.545808 8.2:+40987880 None:intergenic
CTTAGAACAGGGGCAGAAAG+TGG 0.548297 8.2:+40987410 None:intergenic
TTCGCGTCATTTGACATCAA+AGG 0.548380 8.2:-40987741 MS.gene25449:intron
CTGACATATATTCATGTACA+AGG 0.554312 8.2:-40987079 MS.gene25449:CDS
ATTCGCCTTCACCTGGGGAA+AGG 0.554606 8.2:+40987819 None:intergenic
AGATACAGCTTCTTCAGTGA+TGG 0.555293 8.2:+40988100 None:intergenic
TTTCAATCTCTTGGAGCTGG+AGG 0.561546 8.2:+40987952 None:intergenic
GCAACAACCTCGTTTCCCTC+AGG 0.563749 8.2:-40988525 MS.gene25449:intron
CAATGATGCCCAATGTGACA+CGG 0.564076 8.2:+40987879 None:intergenic
CTATGAGTCTTGTGTCTCGT+AGG 0.564360 8.2:-40988822 MS.gene25449:CDS
GATGTCCTTTCCCCAGGTGA+AGG 0.565441 8.2:-40987824 MS.gene25449:CDS
TTCGAATGTTGGGGAATCTG+CGG 0.575364 8.2:+40988695 None:intergenic
TATGGATCCGTACTGCTACT+GGG 0.576454 8.2:-40988618 MS.gene25449:CDS
ACAATCCAGTGTTTCCAGCA+CGG 0.581383 8.2:+40988027 None:intergenic
GGTTGTTGCAGTTGATGAAG+AGG 0.585577 8.2:+40988539 None:intergenic
TGGAAACTTTAATAATATGG+TGG 0.586549 8.2:-40987858 MS.gene25449:CDS
AGAAGATTTGAGTTGAAACA+TGG 0.586977 8.2:+40987344 None:intergenic
CATTCAGAAACTGTTGACTC+TGG 0.589725 8.2:+40987115 None:intergenic
GGTGGGGCCCAGTAGCAGTA+CGG 0.597691 8.2:+40988611 None:intergenic
AACCACCACCATTGAAAACA+TGG 0.601661 8.2:-40987141 MS.gene25449:CDS
TCTACTCGACTTCGATGCTG+TGG 0.604755 8.2:-40988080 MS.gene25449:CDS
TGAGTCTTGTGTCTCGTAGG+TGG 0.607225 8.2:-40988819 MS.gene25449:CDS
GTACATGAATATATGTCAGG+TGG 0.610979 8.2:+40987083 None:intergenic
TACATGAATATATGTCAGGT+GGG 0.613646 8.2:+40987084 None:intergenic
AGATAGAGTTCTTCAAGGTG+GGG 0.613840 8.2:+40988595 None:intergenic
GTTGTTGCAGTTGATGAAGA+GGG 0.613860 8.2:+40988540 None:intergenic
GAGTTCAACAAAGTCATGAG+TGG 0.614644 8.2:+40988570 None:intergenic
TGTGGCATTTCTCAAGCACA+TGG 0.616958 8.2:+40987318 None:intergenic
CTCTTGGAGCTGGAGGAAGG+TGG 0.621502 8.2:+40987959 None:intergenic
CTTCGTCCTCATCGATAAAG+CGG 0.625742 8.2:+40988767 None:intergenic
TAGATTACCTGAGGGAAACG+AGG 0.640585 8.2:+40988518 None:intergenic
GTTAAATTTAAGTTGACACG+TGG 0.645190 8.2:+40986901 None:intergenic
AGACTTGTAGCTGCTGCCAG+AGG 0.652166 8.2:+40988791 None:intergenic
AGAGATAGAGTTCTTCAAGG+TGG 0.653042 8.2:+40988593 None:intergenic
AGTCTTCCGCTTTATCGATG+AGG 0.654779 8.2:-40988773 MS.gene25449:CDS
TGATGAGAACTCGAAGCTTG+TGG 0.658774 8.2:+40987300 None:intergenic
TTCAATGGACAAAACAAACA+AGG 0.664096 8.2:-40986966 MS.gene25449:CDS
GGAAGGTGGAACAGGAACGT+TGG 0.667790 8.2:+40987973 None:intergenic
AATGTTGGGGAATCTGCGGA+GGG 0.668481 8.2:+40988699 None:intergenic
AATCCAGTGTTTCCAGCACG+GGG 0.676114 8.2:+40988029 None:intergenic
TCTCATCATTCAGAGCAGCA+AGG 0.683961 8.2:-40987285 MS.gene25449:intron
ACTCTGGATACCTTAGAACA+GGG 0.693635 8.2:+40987399 None:intergenic
CTCTGGATACCTTAGAACAG+GGG 0.704828 8.2:+40987400 None:intergenic
ATGATGCCCAATGTGACACG+GGG 0.739109 8.2:+40987881 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATATATTTTTGAGTCA+TGG - chr8.2:40988511-40988530 MS.gene25449:intron 15.0%
!!! AGTTTAATTTAATATATGTC+TGG - chr8.2:40988456-40988475 MS.gene25449:intron 15.0%
!!! TATTTTCTTTGATATTTCAA+TGG - chr8.2:40988722-40988741 MS.gene25449:CDS 15.0%
!! AAAGTAGAGAAATATCATAT+TGG + chr8.2:40987944-40987963 None:intergenic 20.0%
!! AAATTCTGGATAAGTTATAA+CGG + chr8.2:40988122-40988141 None:intergenic 20.0%
!! AACCAATTCTACTTATTTAA+AGG + chr8.2:40987246-40987265 None:intergenic 20.0%
!! AACCTTTAAATAAGTAGAAT+TGG - chr8.2:40987241-40987260 MS.gene25449:intron 20.0%
!! CATTGGAAACTTTAATAATA+TGG - chr8.2:40987842-40987861 MS.gene25449:CDS 20.0%
!! CTAATACATGGATAAAAATT+TGG + chr8.2:40987347-40987366 None:intergenic 20.0%
!!! ATGTTAGTAATTAGTTCTTA+TGG - chr8.2:40988010-40988029 MS.gene25449:CDS 20.0%
!!! CATTTGTTGAATATATTTTG+CGG - chr8.2:40988673-40988692 MS.gene25449:CDS 20.0%
!!! GTGAAGTTTTTATTTTTGAA+TGG - chr8.2:40987984-40988003 MS.gene25449:CDS 20.0%
!!! TCATTGTTTTTATTTTTGAC+TGG - chr8.2:40988168-40988187 MS.gene25449:intron 20.0%
! AAAACAAATTAGATTACCTG+AGG + chr8.2:40987197-40987216 None:intergenic 25.0%
! AAACAAATTAGATTACCTGA+GGG + chr8.2:40987196-40987215 None:intergenic 25.0%
! AAATATCATATTGGTCATCT+CGG + chr8.2:40987935-40987954 None:intergenic 25.0%
! AATTCTGGATAAGTTATAAC+GGG + chr8.2:40988121-40988140 None:intergenic 25.0%
! GTTATAACTTATCCAGAATT+TGG - chr8.2:40988121-40988140 MS.gene25449:CDS 25.0%
! TGAAGTAAATTTCCAAATTC+TGG + chr8.2:40988136-40988155 None:intergenic 25.0%
! TGGAAACTTTAATAATATGG+TGG - chr8.2:40987845-40987864 MS.gene25449:CDS 25.0%
!! AGACAAAATTTTCGAATGTT+GGG + chr8.2:40987021-40987040 None:intergenic 25.0%
!! AGATTATCTGAGATAACAAT+TGG - chr8.2:40988774-40988793 MS.gene25449:CDS 25.0%
!! TTAATGCTGGAAATCAATTT+TGG - chr8.2:40987455-40987474 MS.gene25449:intron 25.0%
!!! ATGTTTTTGATATACGTAGT+TGG - chr8.2:40988222-40988241 MS.gene25449:intron 25.0%
AAATACTCATGTCTTAATGC+TGG - chr8.2:40987442-40987461 MS.gene25449:intron 30.0%
ATGTTTCAACTCAAATCTTC+TGG - chr8.2:40988361-40988380 MS.gene25449:intron 30.0%
ATTCATGTACAAGGATATCT+AGG - chr8.2:40988633-40988652 MS.gene25449:CDS 30.0%
ATTGGTAGATTTCAATCTCT+TGG + chr8.2:40987763-40987782 None:intergenic 30.0%
CTGACATATATTCATGTACA+AGG - chr8.2:40988624-40988643 MS.gene25449:CDS 30.0%
CTTGTACATGAATATATGTC+AGG + chr8.2:40988626-40988645 None:intergenic 30.0%
TACATGAATATATGTCAGGT+GGG + chr8.2:40988622-40988641 None:intergenic 30.0%
TTCAATGGACAAAACAAACA+AGG - chr8.2:40988737-40988756 MS.gene25449:CDS 30.0%
TTCAATGTAATCACAAGTGT+TGG + chr8.2:40987312-40987331 None:intergenic 30.0%
TTGTTATCATGTACAATCAG+TGG - chr8.2:40988270-40988289 MS.gene25449:intron 30.0%
! GACAAAATTTTCGAATGTTG+GGG + chr8.2:40987020-40987039 None:intergenic 30.0%
! GAGACAAAATTTTCGAATGT+TGG + chr8.2:40987022-40987041 None:intergenic 30.0%
! GCATTAAGACATGAGTATTT+TGG + chr8.2:40987442-40987461 None:intergenic 30.0%
! GGGAAAACATGTTTAAGTTA+TGG - chr8.2:40987067-40987086 MS.gene25449:CDS 30.0%
! GTTGAATATATTTTGCGGAA+CGG - chr8.2:40988678-40988697 MS.gene25449:CDS 30.0%
! TTAAACATGTTTTCCCAATC+CGG + chr8.2:40987063-40987082 None:intergenic 30.0%
! TTGAATATATTTTGCGGAAC+GGG - chr8.2:40988679-40988698 MS.gene25449:CDS 30.0%
!! AGAAGATTTGAGTTGAAACA+TGG + chr8.2:40988362-40988381 None:intergenic 30.0%
!! ATAAGTAGAATTGGTTTGCA+CGG - chr8.2:40987250-40987269 MS.gene25449:intron 30.0%
!! GTTAAATTTAAGTTGACACG+TGG + chr8.2:40988805-40988824 None:intergenic 30.0%
!!! GAGTATTTTGGCAATTTCAT+AGG + chr8.2:40987430-40987449 None:intergenic 30.0%
!!! TATATTTTTGAGTCATGGCA+TGG - chr8.2:40988516-40988535 MS.gene25449:intron 30.0%
!!! TGTTTTTTGATGTACGTAGT+TGG - chr8.2:40988039-40988058 MS.gene25449:CDS 30.0%
AGTCCAAGTGAAATTGTGAT+TGG + chr8.2:40987781-40987800 None:intergenic 35.0%
CTACCAATCACAATTTCACT+TGG - chr8.2:40987775-40987794 MS.gene25449:CDS 35.0%
CTAGACACAACACTAATACA+TGG + chr8.2:40987359-40987378 None:intergenic 35.0%
GTACATGAATATATGTCAGG+TGG + chr8.2:40988623-40988642 None:intergenic 35.0%
TTGTACATGATAACAAGCGA+TGG + chr8.2:40988266-40988285 None:intergenic 35.0%
! CGATGTCTGTTTTCATTTTC+CGG - chr8.2:40987041-40987060 MS.gene25449:CDS 35.0%
! CTGTTTTCATTTTCCGGATT+GGG - chr8.2:40987047-40987066 MS.gene25449:CDS 35.0%
! TCTGTTTTCATTTTCCGGAT+TGG - chr8.2:40987046-40987065 MS.gene25449:CDS 35.0%
AACCACCACCATTGAAAACA+TGG - chr8.2:40988562-40988581 MS.gene25449:CDS 40.0%
AACTCTGGATACCTTAGAAC+AGG + chr8.2:40988308-40988327 None:intergenic 40.0%
ACGGAGAGAAAGGAAACTAT+TGG + chr8.2:40987651-40987670 None:intergenic 40.0%
ACTCTGGATACCTTAGAACA+GGG + chr8.2:40988307-40988326 None:intergenic 40.0%
AGAGATAGAGTTCTTCAAGG+TGG + chr8.2:40987113-40987132 None:intergenic 40.0%
AGATACAGCTTCTTCAGTGA+TGG + chr8.2:40987606-40987625 None:intergenic 40.0%
AGATAGAGTTCTTCAAGGTG+GGG + chr8.2:40987111-40987130 None:intergenic 40.0%
AGATTTCAATCTCTTGGAGC+TGG + chr8.2:40987757-40987776 None:intergenic 40.0%
GAGATAGAGTTCTTCAAGGT+GGG + chr8.2:40987112-40987131 None:intergenic 40.0%
GAGTTCAACAAAGTCATGAG+TGG + chr8.2:40987136-40987155 None:intergenic 40.0%
TGCTTGTACACGATAACAAC+AGG + chr8.2:40988084-40988103 None:intergenic 40.0%
TGGAGAGATAGAGTTCTTCA+AGG + chr8.2:40987116-40987135 None:intergenic 40.0%
TTCGCGTCATTTGACATCAA+AGG - chr8.2:40987962-40987981 MS.gene25449:CDS 40.0%
TTCTGATTGAATGCATGTCC+CGG - chr8.2:40987277-40987296 MS.gene25449:intron 40.0%
! CGATCTAATTTGCGAAACTC+TGG + chr8.2:40988323-40988342 None:intergenic 40.0%
! CTCGGATGTCTTTGAGAATA+GGG + chr8.2:40987917-40987936 None:intergenic 40.0%
! GACTGGCTAATGCTAGTAAT+TGG - chr8.2:40988185-40988204 MS.gene25449:intron 40.0%
! TCTCGGATGTCTTTGAGAAT+AGG + chr8.2:40987918-40987937 None:intergenic 40.0%
!! AGCACAGTGTTGACGAATAA+TGG + chr8.2:40986982-40987001 None:intergenic 40.0%
!! CATTCAGAAACTGTTGACTC+TGG + chr8.2:40988591-40988610 None:intergenic 40.0%
!! GTAATCACAAGTGTTGGTGT+CGG + chr8.2:40987306-40987325 None:intergenic 40.0%
!! GTTGTTGCAGTTGATGAAGA+GGG + chr8.2:40987166-40987185 None:intergenic 40.0%
AATGATGCCCAATGTGACAC+GGG + chr8.2:40987826-40987845 None:intergenic 45.0%
ACAATCCAGTGTTTCCAGCA+CGG + chr8.2:40987679-40987698 None:intergenic 45.0%
AGTCTTCCGCTTTATCGATG+AGG - chr8.2:40986930-40986949 MS.gene25449:CDS 45.0%
ATAGATCCCCGTGTCACATT+GGG - chr8.2:40987816-40987835 MS.gene25449:CDS 45.0%
CAATGATGCCCAATGTGACA+CGG + chr8.2:40987827-40987846 None:intergenic 45.0%
CATGATAACAAGCGATGGTG+CGG + chr8.2:40988261-40988280 None:intergenic 45.0%
CGAAAAATTCGCCTTCACCT+GGG + chr8.2:40987893-40987912 None:intergenic 45.0%
CTCTGGATACCTTAGAACAG+GGG + chr8.2:40988306-40988325 None:intergenic 45.0%
CTTCGTCCTCATCGATAAAG+CGG + chr8.2:40986939-40986958 None:intergenic 45.0%
GAAAAATTCGCCTTCACCTG+GGG + chr8.2:40987892-40987911 None:intergenic 45.0%
TACACGATAACAACAGGCGA+TGG + chr8.2:40988078-40988097 None:intergenic 45.0%
TAGATTACCTGAGGGAAACG+AGG + chr8.2:40987188-40987207 None:intergenic 45.0%
TATAGATCCCCGTGTCACAT+TGG - chr8.2:40987815-40987834 MS.gene25449:CDS 45.0%
TATGGATCCGTACTGCTACT+GGG - chr8.2:40987085-40987104 MS.gene25449:CDS 45.0%
TCGAAAAATTCGCCTTCACC+TGG + chr8.2:40987894-40987913 None:intergenic 45.0%
TCTCATCATTCAGAGCAGCA+AGG - chr8.2:40988418-40988437 MS.gene25449:intron 45.0%
TGATGAGAACTCGAAGCTTG+TGG + chr8.2:40988406-40988425 None:intergenic 45.0%
TGTGGCATTTCTCAAGCACA+TGG + chr8.2:40988388-40988407 None:intergenic 45.0%
TTATGGATCCGTACTGCTAC+TGG - chr8.2:40987084-40987103 MS.gene25449:CDS 45.0%
TTCGAATGTTGGGGAATCTG+CGG + chr8.2:40987011-40987030 None:intergenic 45.0%
TTTCAATCTCTTGGAGCTGG+AGG + chr8.2:40987754-40987773 None:intergenic 45.0%
! TCTGGCTGCCATGTTTTCAA+TGG + chr8.2:40988573-40988592 None:intergenic 45.0%
! TGCCATGTTTTCAATGGTGG+TGG + chr8.2:40988567-40988586 None:intergenic 45.0%
!! AAGTTATAACGGGCAGGAAG+TGG + chr8.2:40988111-40988130 None:intergenic 45.0%
!! AGTTATAACGGGCAGGAAGT+GGG + chr8.2:40988110-40988129 None:intergenic 45.0%
!! CTGGATAAGTTATAACGGGC+AGG + chr8.2:40988117-40988136 None:intergenic 45.0%
!! GGTTGTTGCAGTTGATGAAG+AGG + chr8.2:40987167-40987186 None:intergenic 45.0%
AATCCAGTGTTTCCAGCACG+GGG + chr8.2:40987677-40987696 None:intergenic 50.0%
AATCTCTTGGAGCTGGAGGA+AGG + chr8.2:40987750-40987769 None:intergenic 50.0%
AATGTTGGGGAATCTGCGGA+GGG + chr8.2:40987007-40987026 None:intergenic 50.0%
ATGATGCCCAATGTGACACG+GGG + chr8.2:40987825-40987844 None:intergenic 50.0%
CAATCCAGTGTTTCCAGCAC+GGG + chr8.2:40987678-40987697 None:intergenic 50.0%
CACTTTCTGCCCCTGTTCTA+AGG - chr8.2:40988294-40988313 MS.gene25449:intron 50.0%
CGTGTCACATTGGGCATCAT+TGG - chr8.2:40987825-40987844 MS.gene25449:CDS 50.0%
CTAGCGTGTGTGTGCTTCAT+AGG - chr8.2:40987375-40987394 MS.gene25449:CDS 50.0%
CTTAGAACAGGGGCAGAAAG+TGG + chr8.2:40988296-40988315 None:intergenic 50.0%
TCTACTCGACTTCGATGCTG+TGG - chr8.2:40987623-40987642 MS.gene25449:intron 50.0%
TGGTGTCTCTTGTTGCTGGT+AGG - chr8.2:40988536-40988555 MS.gene25449:CDS 50.0%
TGTCTCTTGTTGCTGGTAGG+AGG - chr8.2:40988539-40988558 MS.gene25449:CDS 50.0%
TTTCTCGATGTCCTTTCCCC+AGG - chr8.2:40987873-40987892 MS.gene25449:CDS 50.0%
! GGCTGCCATGTTTTCAATGG+TGG + chr8.2:40988570-40988589 None:intergenic 50.0%
! TGAGTCTTGTGTCTCGTAGG+TGG - chr8.2:40986884-40986903 MS.gene25449:CDS 50.0%
!! TTTGCGGAACGGGCTTGTTT+TGG - chr8.2:40988689-40988708 MS.gene25449:CDS 50.0%
AGACTTGTAGCTGCTGCCAG+AGG + chr8.2:40986915-40986934 None:intergenic 55.0%
ATTCGCCTTCACCTGGGGAA+AGG + chr8.2:40987887-40987906 None:intergenic 55.0%
GAATGTTGGGGAATCTGCGG+AGG + chr8.2:40987008-40987027 None:intergenic 55.0%
GATGTCCTTTCCCCAGGTGA+AGG - chr8.2:40987879-40987898 MS.gene25449:CDS 55.0%
GCAACAACCTCGTTTCCCTC+AGG - chr8.2:40987178-40987197 MS.gene25449:intron 55.0%
GGAAGGTGGAACAGGAACGT+TGG + chr8.2:40987733-40987752 None:intergenic 55.0%
GGCATGGTGTCTCTTGTTGC+TGG - chr8.2:40988532-40988551 MS.gene25449:CDS 55.0%
!! AAGTGTTGGTGTCGGACACC+GGG + chr8.2:40987298-40987317 None:intergenic 55.0%
!! CAAGTGTTGGTGTCGGACAC+CGG + chr8.2:40987299-40987318 None:intergenic 55.0%
CTCTTGGAGCTGGAGGAAGG+TGG + chr8.2:40987747-40987766 None:intergenic 60.0%
CTTGTAGCTGCTGCCAGAGG+TGG + chr8.2:40986912-40986931 None:intergenic 60.0%
GAGCTGGAGGAAGGTGGAAC+AGG + chr8.2:40987741-40987760 None:intergenic 60.0%
TGTTTCCAGCACGGGGCAGA+CGG + chr8.2:40987670-40987689 None:intergenic 60.0%
CACGGGGCAGACGGAGAGAA+AGG + chr8.2:40987661-40987680 None:intergenic 65.0%
CTGCCCCGTGCTGGAAACAC+TGG - chr8.2:40987671-40987690 MS.gene25449:intron 65.0%
GGTGGGGCCCAGTAGCAGTA+CGG + chr8.2:40987095-40987114 None:intergenic 65.0%
CTCGTAGGTGGCGCCACCTC+TGG - chr8.2:40986896-40986915 MS.gene25449:CDS 70.0%
TCTCTCCGTCTGCCCCGTGC+TGG - chr8.2:40987662-40987681 MS.gene25449:intron 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 40986883 40988842 40986883 ID=MS.gene25449
chr8.2 mRNA 40986883 40988842 40986883 ID=MS.gene25449.t1;Parent=MS.gene25449
chr8.2 exon 40988526 40988842 40988526 ID=MS.gene25449.t1.exon1;Parent=MS.gene25449.t1
chr8.2 CDS 40988526 40988842 40988526 ID=cds.MS.gene25449.t1;Parent=MS.gene25449.t1
chr8.2 exon 40987742 40988174 40987742 ID=MS.gene25449.t1.exon2;Parent=MS.gene25449.t1
chr8.2 CDS 40987742 40988174 40987742 ID=cds.MS.gene25449.t1;Parent=MS.gene25449.t1
chr8.2 exon 40987286 40987435 40987286 ID=MS.gene25449.t1.exon3;Parent=MS.gene25449.t1
chr8.2 CDS 40987286 40987435 40987286 ID=cds.MS.gene25449.t1;Parent=MS.gene25449.t1
chr8.2 exon 40986883 40987167 40986883 ID=MS.gene25449.t1.exon4;Parent=MS.gene25449.t1
chr8.2 CDS 40986883 40987167 40986883 ID=cds.MS.gene25449.t1;Parent=MS.gene25449.t1
Gene Sequence

>MS.gene25449

ATGAGTCTTGTGTCTCGTAGGTGGCGCCACCTCTGGCAGCAGCTACAAGTCTTCCGCTTTATCGATGAGGACGAAGACGTAGAGTTCAATGACTTTCCATTATTCGTCAACACTGTGCTCACCCTCCGCAGATTCCCCAACATTCGAAAATTTTGTCTCGATGTCTGTTTTCATTTTCCGGATTGGGAAAACATGTTTAAGTTATGGATCCGTACTGCTACTGGGCCCCACCTTGAAGAACTCTATCTCTCCACTCATGACTTTGTTGAACTCTCTTCTACCCTCTTCATCAACTGCAACAACCTCGTTTCCCTCAGGTAATCTAATTTGTTTTCGCTAATAGCTAGTTTCATTCAAAAACCTTTAAATAAGTAGAATTGGTTTGCACGGAGACTTCTGATTGAATGCATGTCCCGGTGTCCGACACCAACACTTGTGATTACATTGAATTATTTAGTTTTCCAAATTTTTATCCATGTATTAGTGTTGTGTCTAGCGTGTGTGTGCTTCATAGGTCTGACTTCTGTTTCAAAATTGTTTTAATCCTATGAAATTGCCAAAATACTCATGTCTTAATGCTGGAAATCAATTTTGGTATAAATTTTACATATTATCTTCAAATAGACAATGTTATATACAATCTCTCTTGTCTCTTGAAAACACTGCAGTCTTACGTGTGTAGAAATCAAAATTAAAGTTCAACATTGTTCATTTCATTTTCCATCACTGAAGAAGCTGTATCTACTCGACTTCGATGCTGTGGATCCAATAGTTTCCTTTCTCTCCGTCTGCCCCGTGCTGGAAACACTGGATTGTGATTACTTTTGCTATAAAGACATATCTTTGACCAACGTTCCTGTTCCACCTTCCTCCAGCTCCAAGAGATTGAAATCTACCAATCACAATTTCACTTGGACTTATTTTAAGTTAGATATAGATCCCCGTGTCACATTGGGCATCATTGGAAACTTTAATAATATGGTGGAAGCATTTCTCGATGTCCTTTCCCCAGGTGAAGGCGAATTTTTCGACCCTATTCTCAAAGACATCCGAGATGACCAATATGATATTTCTCTACTTTCGCGTCATTTGACATCAAAGGTGAAGTTTTTATTTTTGAATGGAAAATGTTAGTAATTAGTTCTTATGGTTTATATGTTTTTTGATGTACGTAGTTGGTACTAATATTGCACCATCGCCTGTTGTTATCGTGTACAAGCAGTGCCCACTTCCTGCCCGTTATAACTTATCCAGAATTTGGAAATTTACTTCATCTAAATTTATTTCATTGTTTTTATTTTTGACTGGCTAATGCTAGTAATTGGTTGTTATGCATTATATGTTTTTGATATACGTAGTTGGTACTAATATTGCACCGCACCATCGCTTGTTATCATGTACAATCAGTGGCCACTTTCTGCCCCTGTTCTAAGGTATCCAGAGTTTCGCAAATTAGATCGTCTAAAGTTCATTCTTCCATGTTTCAACTCAAATCTTCTGGTCCATGTGCTTGAGAAATGCCACAAGCTTCGAGTTCTCATCATTCAGAGCAGCAAGGTATGTTGACTTCTCAAGTTTAATTTAATATATGTCTGGTTTGTCTATATATGCATGCAATCTATCTGTGCAAAAATATATTTTTGAGTCATGGCATGGTGTCTCTTGTTGCTGGTAGGAGGAACCACCACCATTGAAAACATGGCAGCCAGAGTCAACAGTTTCTGAATGTCTCAAATCCCACCTGACATATATTCATGTACAAGGATATCTAGGACTTGAAGATGAGTTAACATTTGTTGAATATATTTTGCGGAACGGGCTTGTTTTGGAGACAGTGCTTATTTTCTTTGATATTTCAATGGACAAAACAAACAAGGACTGCTCGTTCAAAAGATTATCTGAGATAACAATTGGTTCTTCCACGTGTCAACTTAAATTTAACCATGTTATATCTCCTTAA
Protein sequence

>MS.gene25449.t1

MSLVSRRWRHLWQQLQVFRFIDEDEDVEFNDFPLFVNTVLTLRRFPNIRKFCLDVCFHFPDWENMFKLWIRTATGPHLEELYLSTHDFVELSSTLFINCNNLVSLSLTCVEIKIKVQHCSFHFPSLKKLYLLDFDAVDPIVSFLSVCPVLETLDCDYFCYKDISLTNVPVPPSSSSKRLKSTNHNFTWTYFKLDIDPRVTLGIIGNFNNMVEAFLDVLSPGEGEFFDPILKDIRDDQYDISLLSRHLTSKWPLSAPVLRYPEFRKLDRLKFILPCFNSNLLVHVLEKCHKLRVLIIQSSKEEPPPLKTWQPESTVSECLKSHLTYIHVQGYLGLEDELTFVEYILRNGLVLETVLIFFDISMDKTNKDCSFKRLSEITIGSSTCQLKFNHVISP