AlfalfaGEDB | MS.gene64992

M. sativa cultivar XinJiangDaYe / MS.gene64992

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00646.36 PF08387.13

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64992.t1	XP_003628587.2	64.3	465	139	7	1	455	464	911	9.80E-148	533.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64992.t1	Q9SV82	26.4	447	277	16	20	454	2	408	1.7e-15	85.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64992.t1	G7LEM0	60.7	494	138	9	1	455	1	477	3.1e-143	518.1

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene002112	MS.gene64992	PPI
MS.gene003456	MS.gene64992	PPI
MS.gene004045	MS.gene64992	PPI
MS.gene005794	MS.gene64992	PPI
MS.gene005795	MS.gene64992	PPI
MS.gene006692	MS.gene64992	PPI
MS.gene006725	MS.gene64992	PPI
MS.gene006727	MS.gene64992	PPI
MS.gene006728	MS.gene64992	PPI
MS.gene006730	MS.gene64992	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64992.t1	MTR_8g062300	60.729	494	138	9	1	455	1	477	0.0	530
MS.gene64992.t1	MTR_8g070950	58.442	462	154	11	3	455	2	434	3.40e-159	457
MS.gene64992.t1	MTR_8g062010	57.650	451	163	12	12	455	1	430	1.94e-150	435
MS.gene64992.t1	MTR_8g062310	54.422	441	167	8	20	459	25	432	9.32e-149	431
MS.gene64992.t1	MTR_8g062080	53.509	456	183	9	8	455	11	445	6.07e-147	427
MS.gene64992.t1	MTR_8g469020	47.447	470	192	10	18	455	5	451	2.45e-134	395
MS.gene64992.t1	MTR_8g058965	48.954	478	189	13	11	455	1	456	1.78e-130	385
MS.gene64992.t1	MTR_4g050250	47.285	442	170	15	1	401	1	420	6.23e-110	332
MS.gene64992.t1	MTR_8g467790	45.960	396	150	12	20	400	148	494	1.27e-93	292
MS.gene64992.t1	MTR_8g468810	38.797	482	244	16	4	455	3	463	2.73e-75	243

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64992.t1	AT4G10400	27.902	448	269	18	20	454	2	408	1.13e-21	97.1
MS.gene64992.t1	AT4G10400	27.902	448	269	18	20	454	2	408	1.13e-21	97.1
MS.gene64992.t1	AT4G10400	27.902	448	269	18	20	454	2	408	1.13e-21	97.1
MS.gene64992.t1	AT1G78750	26.050	476	303	19	4	452	2	455	2.60e-18	87.4
MS.gene64992.t1	AT1G78750	26.050	476	303	19	4	452	2	455	2.60e-18	87.4
MS.gene64992.t1	AT1G32375	27.331	472	256	21	20	455	2	422	2.09e-17	84.7
MS.gene64992.t1	AT1G32375	27.331	472	256	21	20	455	2	422	2.09e-17	84.7
MS.gene64992.t1	AT5G50270	28.246	439	241	18	20	426	2	398	5.91e-16	80.1
MS.gene64992.t1	AT4G00160	27.983	461	283	19	20	460	16	447	7.74e-16	80.1
MS.gene64992.t1	AT4G09920	33.533	167	100	5	20	183	2	160	1.37e-15	77.8

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Find 111 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATAATGAATGTATTAGAT+TGG	0.376850	8.4:+39626489	MS.gene64992:CDS
AACCACTGCCATTGAGAACA+TGG	0.558537	8.4:+39627072	MS.gene64992:CDS
AACCCGCAAAACTTCCAACT+TGG	0.473471	8.4:-39626344	None:intergenic
AACCTAATCGACGATGGCAA+CGG	0.562160	8.4:+39625632	None:intergenic
AACTTAAATTTGACGTTGAT+GGG	0.410278	8.4:+39627324	MS.gene64992:CDS
AAGATTAACGAATATATCAA+AGG	0.534259	8.4:+39627286	MS.gene64992:CDS
AATAATGAATGTATTAGATT+GGG	0.455243	8.4:+39626490	MS.gene64992:CDS
AATGTCGCGGAATTTGCGGA+GGG	0.690292	8.4:-39625901	None:intergenic
AATTGGTCAAGAGGGAAGGA+GGG	0.570036	8.4:-39626070	None:intergenic
ACCCGCAAAACTTCCAACTT+GGG	0.570628	8.4:-39626343	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAACTTAAATTTGACGTTGA+TGG	+	chr8.4:39627323-39627342	MS.gene64992:CDS	30.0%
CATCAACGTCAAATTTAAGT+TGG	-	chr8.4:39627325-39627344	None:intergenic	30.0%
GAAATGTTAGAAGTGAGTTT+GGG	-	chr8.4:39626137-39626156	None:intergenic	30.0%
TAGATTCACATTCGTTCAAA+AGG	+	chr8.4:39625850-39625869	MS.gene64992:CDS	30.0%
TGAAATGTTAGAAGTGAGTT+TGG	-	chr8.4:39626138-39626157	None:intergenic	30.0%
TGAACTTCAGATGATGTAAA+CGG	-	chr8.4:39626825-39626844	None:intergenic	30.0%
TGTAGGTTCTTATAGTATAG+AGG	+	chr8.4:39626222-39626241	MS.gene64992:CDS	30.0%
TTTGAGCAATGGAAAATGAA+CGG	-	chr8.4:39626260-39626279	None:intergenic	30.0%
ACTGCTATGATGTATATCTG+CGG	+	chr8.4:39626650-39626669	MS.gene64992:CDS	35.0%
AGTCCAAGTGAAATTGTCAT+TGG	-	chr8.4:39626449-39626468	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	39625645	39627355	39625645	ID=MS.gene64992
chr8.4	mRNA	39625645	39627355	39625645	ID=MS.gene64992.t1;Parent=MS.gene64992
chr8.4	exon	39625645	39626114	39625645	ID=MS.gene64992.t1.exon1;Parent=MS.gene64992.t1
chr8.4	CDS	39625645	39626114	39625645	ID=cds.MS.gene64992.t1;Parent=MS.gene64992.t1
chr8.4	exon	39626219	39626693	39626219	ID=MS.gene64992.t1.exon2;Parent=MS.gene64992.t1
chr8.4	CDS	39626219	39626693	39626219	ID=cds.MS.gene64992.t1;Parent=MS.gene64992.t1
chr8.4	exon	39626781	39626930	39626781	ID=MS.gene64992.t1.exon3;Parent=MS.gene64992.t1
chr8.4	CDS	39626781	39626930	39626781	ID=cds.MS.gene64992.t1;Parent=MS.gene64992.t1
chr8.4	exon	39627068	39627355	39627068	ID=MS.gene64992.t1.exon4;Parent=MS.gene64992.t1
chr8.4	CDS	39627068	39627355	39627068	ID=cds.MS.gene64992.t1;Parent=MS.gene64992.t1

Gene Sequence

>MS.gene64992

ATGGCAACGGAGTCAAGCATGGAACGATCGCTTGTTGCAGAAGAAGACAACGTCACGGACAGATTGAGCAGTCTACCGGACTCGATCCTGTGTCATATCATGTCCTTCCTCCCTACCACAACCTCCGTCGCTATGATGAGAGTCTCTCGCCGGTGGCGCCACCTCTGGAAGGATCTCCAGATATTCAACTTCTGCAGTCGCAATCTAGATTCACATTCGTTCAAAAGGTTTTTATTATTCGTGAACGCTGTACTTACCCTCCGCAAATTCCGCGACATTCATAAGTTCCATCTCATACTCGGCTTTAATCGATCCGTTTGTGACAGCTTTCATCTCGCTTGTATTCGTACGTGGATCCTTGCTGCCATTGGACCCCACCTTCAACAACTCTATCTCTACATTGTCAATGCTGCTGTTGTAGTTCTCCCTCCTTCCCTCTTGACCAATTGCTCCAACCTCCTTTACCTCAGGTTATAATTGCTTTTTATGCCCAAACTCACTTCTAACATTTCATCTCTTGTTTTTTTTACTTTTTTTTTTGTTCTTTCCTAACATGTATGTCTTTTGAAAACAGTCTTGTAGGTTCTTATAGTATAGAGGTAAAACAGTCTTCCGTTCATTTTCCATTGCTCAAAGTGCTGTCTCTCGCCGCAGAAATTGTTAGTCTAAAAGAAATAGTTTACCTTCTATTTGGCAGTCCCAAGTTGGAAGTTTTGCGGGTTTACTTTTACTTTATCTCTGAAGAACTTGTGCCCAAAGCTTTAATTCCACCTTCCTCCAGCTCCGAGAGCTCCAAATCGGCCAATGACAATTTCACTTGGACTTACTTTAAAATAGTTAACACAAATAATGAATGTATTAGATTGGGCATTGTTGGCAACTTTCACAGTATGGGGGAAGCATTTCTTGATGTCTTTTCCTTGACTGAAAGTGAATTTGTCGACCCTATTCTCAACCTCATCCGAGATGATGGCAGAACTGAATTTTATAAAAACCTCCCAGCTGACTGCTATGATGTATATCTGCGGTTGCGTCATTCAACATCAAAGGTGATGTTTTATTCTTATGGTTTATATGTATATCTACGGTTTGGTACTAATAGTGCGTCATCACTTGTTGTTATCGTGTACCTGCAGTGGCGACCTCATGCCCGTGTTCTAAATTATCCTGAATTTAGCCGTTTACATCATCTGAAGTTCATTCTTCCATGTTTCGACTCAAATCTGCTGGTCCATGTTCTTGAGCAATGCCACATGCTTCAAGTTCTCATAATTCAGAGCAACAAGGTTTGTTGACTTCTCAAGTTTAATACAAACAATCTTTGGTCTGCCTATATATACATGTATGTATGTATGTATGTATGTATCTATCTGTACACCAATATATTTTTGAGTAATGACCTGGTTTCCCTTGTTGGTGGTAGGAGGAACCACTGCCATTGAGAACATGGCAGCTGGAGTCAACAACAGTTTTTGAATGTCTAAAATCCCACCTGACTTATGTTCACATAGAAGGATATCAAGGACTTGAAGATGAGTTCACATTTGCTGAATATATTTTGCGGAATGGACTTCTTTTGAAGACGATGCTTATTTTCGTTGATAGTTCAATAGACAAAACGGACATGGATTGTTCTCTCAAAAGATTAACGAATATATCAAAGGGCTCTGTTGCTTGCCAACTTAAATTTGACGTTGATGGGTCTCATTAA

Protein sequence

>MS.gene64992.t1

MATESSMERSLVAEEDNVTDRLSSLPDSILCHIMSFLPTTTSVAMMRVSRRWRHLWKDLQIFNFCSRNLDSHSFKRFLLFVNAVLTLRKFRDIHKFHLILGFNRSVCDSFHLACIRTWILAAIGPHLQQLYLYIVNAAVVVLPPSLLTNCSNLLYLSLVGSYSIEVKQSSVHFPLLKVLSLAAEIVSLKEIVYLLFGSPKLEVLRVYFYFISEELVPKALIPPSSSSESSKSANDNFTWTYFKIVNTNNECIRLGIVGNFHSMGEAFLDVFSLTESEFVDPILNLIRDDGRTEFYKNLPADCYDVYLRLRHSTSKWRPHARVLNYPEFSRLHHLKFILPCFDSNLLVHVLEQCHMLQVLIIQSNKEEPLPLRTWQLESTTVFECLKSHLTYVHIEGYQGLEDEFTFAEYILRNGLLLKTMLIFVDSSIDKTDMDCSLKRLTNISKGSVACQLKFDVDGSH