AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64992

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64992.t1 MTR_8g062300 60.729 494 138 9 1 455 1 477 0.0 530
MS.gene64992.t1 MTR_8g070950 58.442 462 154 11 3 455 2 434 3.40e-159 457
MS.gene64992.t1 MTR_8g062010 57.650 451 163 12 12 455 1 430 1.94e-150 435
MS.gene64992.t1 MTR_8g062310 54.422 441 167 8 20 459 25 432 9.32e-149 431
MS.gene64992.t1 MTR_8g062080 53.509 456 183 9 8 455 11 445 6.07e-147 427
MS.gene64992.t1 MTR_8g469020 47.447 470 192 10 18 455 5 451 2.45e-134 395
MS.gene64992.t1 MTR_8g058965 48.954 478 189 13 11 455 1 456 1.78e-130 385
MS.gene64992.t1 MTR_4g050250 47.285 442 170 15 1 401 1 420 6.23e-110 332
MS.gene64992.t1 MTR_8g467790 45.960 396 150 12 20 400 148 494 1.27e-93 292
MS.gene64992.t1 MTR_8g468810 38.797 482 244 16 4 455 3 463 2.73e-75 243
MS.gene64992.t1 MTR_2g083840 38.186 474 245 14 6 455 8 457 1.55e-74 241
MS.gene64992.t1 MTR_8g062270 36.717 463 240 14 20 454 16 453 4.81e-72 234
MS.gene64992.t1 MTR_8g058970 37.475 491 252 16 4 455 3 477 4.53e-71 233
MS.gene64992.t1 MTR_2g086020 79.433 141 29 0 315 455 175 315 4.16e-68 220
MS.gene64992.t1 MTR_2g086020 49.359 156 73 3 45 197 1 153 9.45e-35 132
MS.gene64992.t1 MTR_8g014260 35.307 473 266 16 1 455 1 451 6.82e-67 221
MS.gene64992.t1 MTR_8g467800 35.220 477 261 14 8 459 4 457 2.48e-63 212
MS.gene64992.t1 MTR_8g468840 36.484 455 252 13 18 453 12 448 2.08e-62 209
MS.gene64992.t1 MTR_4g032730 33.401 491 255 14 8 454 4 466 2.63e-62 209
MS.gene64992.t1 MTR_5g092960 33.686 472 257 11 15 454 14 461 5.71e-60 203
MS.gene64992.t1 MTR_8g468760 34.395 471 228 14 12 454 1 418 2.73e-55 190
MS.gene64992.t1 MTR_8g468810 38.974 390 189 15 4 365 3 371 7.27e-54 185
MS.gene64992.t1 MTR_8g445780 36.364 407 212 15 20 408 13 390 1.11e-51 179
MS.gene64992.t1 MTR_8g468810 40.717 307 149 11 4 287 3 299 1.75e-45 161
MS.gene64992.t1 MTR_8g468810 40.717 307 149 11 4 287 3 299 2.63e-45 160
MS.gene64992.t1 MTR_8g468810 40.717 307 149 11 4 287 3 299 5.68e-45 160
MS.gene64992.t1 MTR_8g014160 30.719 459 221 15 20 455 41 425 8.53e-40 148
MS.gene64992.t1 MTR_5g092960 33.245 379 204 9 15 365 14 371 2.51e-39 146
MS.gene64992.t1 MTR_3g099800 70.115 87 25 1 367 452 19 105 1.10e-32 120
MS.gene64992.t1 MTR_8g467800 36.000 300 162 8 8 287 4 293 8.48e-32 124
MS.gene64992.t1 MTR_4g024710 39.500 200 104 6 257 455 164 347 9.03e-31 122
MS.gene64992.t1 MTR_8g077460 38.426 216 117 8 20 224 10 220 7.83e-29 117
MS.gene64992.t1 MTR_8g077460 41.722 151 80 5 308 455 230 375 3.76e-26 109
MS.gene64992.t1 MTR_5g021550 43.373 166 73 7 5 156 4 162 2.02e-27 107
MS.gene64992.t1 MTR_8g014210 38.500 200 102 7 259 455 50 231 2.55e-27 109
MS.gene64992.t1 MTR_1g041245 28.062 449 237 16 20 455 11 386 8.59e-25 105
MS.gene64992.t1 MTR_8g014290 38.372 172 79 7 1 157 1 160 5.43e-24 98.2
MS.gene64992.t1 MTR_7g098510 26.226 469 254 18 1 455 1 391 4.08e-22 98.2
MS.gene64992.t1 MTR_7g026500 25.840 476 253 17 7 459 1 399 3.09e-20 92.8
MS.gene64992.t1 MTR_4g024745 30.249 281 148 11 169 430 25 276 4.08e-20 90.5
MS.gene64992.t1 MTR_5g084070 27.051 451 284 18 8 447 3 419 2.21e-19 90.5
MS.gene64992.t1 MTR_4g024720 38.760 129 68 4 4 125 3 127 6.78e-19 85.5
MS.gene64992.t1 MTR_5g080710 27.314 443 263 17 3 427 24 425 2.18e-18 87.8
MS.gene64992.t1 MTR_7g056447 34.010 197 114 6 12 203 1 186 3.01e-17 82.4
MS.gene64992.t1 MTR_7g098480 28.913 460 249 18 1 454 1 388 7.83e-17 83.2
MS.gene64992.t1 MTR_7g055943 24.790 476 262 21 2 456 22 422 2.71e-16 81.3
MS.gene64992.t1 MTR_5g073420 32.632 190 118 7 20 204 11 195 3.27e-16 80.5
MS.gene64992.t1 MTR_3g107120 33.500 200 112 9 20 204 9 202 8.38e-16 79.3
MS.gene64992.t1 MTR_2g007400 24.893 466 252 17 17 455 9 403 8.95e-16 79.3
MS.gene64992.t1 MTR_1g108820 27.033 455 243 21 14 452 2 383 1.51e-15 78.6
MS.gene64992.t1 MTR_2g008310 26.444 450 237 17 20 455 2 371 1.54e-15 78.6
MS.gene64992.t1 MTR_6g045333 24.777 448 261 14 20 454 61 445 2.60e-15 78.2
MS.gene64992.t1 MTR_5g008950 31.925 213 123 9 22 229 1 196 1.60e-14 75.5
MS.gene64992.t1 MTR_4g024770 50.000 64 29 1 1 64 8 68 2.48e-14 68.6
MS.gene64992.t1 MTR_7g115270 31.553 206 119 7 20 212 12 208 2.66e-14 74.7
MS.gene64992.t1 MTR_7g032380 34.536 194 109 7 20 206 11 193 2.96e-14 74.3
MS.gene64992.t1 MTR_5g008920 24.622 463 255 18 7 456 1 382 5.45e-14 73.9
MS.gene64992.t1 MTR_2g007500 29.952 207 118 7 7 206 1 187 6.16e-14 73.2
MS.gene64992.t1 MTR_7g034110 46.067 89 43 3 20 108 11 94 3.77e-13 70.9
MS.gene64992.t1 MTR_2g008320 32.195 205 102 6 20 206 52 237 4.27e-13 71.2
MS.gene64992.t1 MTR_8g014180 55.172 58 24 1 4 61 3 58 4.78e-13 64.3
MS.gene64992.t1 MTR_7g033990 31.100 209 115 8 17 211 8 201 5.67e-13 70.9
MS.gene64992.t1 MTR_7g075820 22.467 454 263 13 20 452 6 391 7.16e-13 70.5
MS.gene64992.t1 MTR_1g492880 39.785 93 52 2 12 103 1 90 7.30e-13 64.7
MS.gene64992.t1 MTR_1g492880 39.785 93 52 2 12 103 1 90 9.03e-13 65.1
MS.gene64992.t1 MTR_3g023630 32.143 196 109 9 19 204 7 188 1.04e-12 69.7
MS.gene64992.t1 MTR_6g088705 35.714 140 81 4 7 143 6 139 1.39e-12 67.8
MS.gene64992.t1 MTR_3g105060 32.143 196 122 9 20 212 14 201 2.90e-12 68.6
MS.gene64992.t1 MTR_5g083890 30.653 199 122 5 20 202 6 204 3.25e-12 68.9
MS.gene64992.t1 MTR_7g033945 32.143 168 101 7 20 181 11 171 5.20e-12 64.7
MS.gene64992.t1 MTR_8g014230 40.000 85 47 2 364 448 18 98 5.23e-12 62.8
MS.gene64992.t1 MTR_7g056433 33.019 212 114 9 7 206 1 196 5.63e-12 67.8
MS.gene64992.t1 MTR_3g107100 29.208 202 131 9 10 207 2 195 7.08e-12 67.4
MS.gene64992.t1 MTR_3g040700 28.125 192 128 5 19 206 42 227 1.63e-11 66.2
MS.gene64992.t1 MTR_4g098590 31.313 198 118 8 22 206 1 193 1.82e-11 65.9
MS.gene64992.t1 MTR_3g036250 29.592 196 120 7 19 206 4 189 4.83e-11 64.7
MS.gene64992.t1 MTR_8g085430 31.658 199 115 6 20 206 11 200 5.04e-11 64.3
MS.gene64992.t1 MTR_2g007450 30.568 229 124 10 9 210 5 225 7.17e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64992.t1 AT4G10400 27.902 448 269 18 20 454 2 408 1.13e-21 97.1
MS.gene64992.t1 AT4G10400 27.902 448 269 18 20 454 2 408 1.13e-21 97.1
MS.gene64992.t1 AT4G10400 27.902 448 269 18 20 454 2 408 1.13e-21 97.1
MS.gene64992.t1 AT1G78750 26.050 476 303 19 4 452 2 455 2.60e-18 87.4
MS.gene64992.t1 AT1G78750 26.050 476 303 19 4 452 2 455 2.60e-18 87.4
MS.gene64992.t1 AT1G32375 27.331 472 256 21 20 455 2 422 2.09e-17 84.7
MS.gene64992.t1 AT1G32375 27.331 472 256 21 20 455 2 422 2.09e-17 84.7
MS.gene64992.t1 AT5G50270 28.246 439 241 18 20 426 2 398 5.91e-16 80.1
MS.gene64992.t1 AT4G00160 27.983 461 283 19 20 460 16 447 7.74e-16 80.1
MS.gene64992.t1 AT4G09920 33.533 167 100 5 20 183 2 160 1.37e-15 77.8
MS.gene64992.t1 AT4G09920 33.533 167 100 5 20 183 2 160 1.37e-15 77.8
MS.gene64992.t1 AT4G09920 33.533 167 100 5 20 183 2 160 2.04e-15 77.0
MS.gene64992.t1 AT4G09920 33.533 167 100 5 20 183 2 160 2.04e-15 77.0
MS.gene64992.t1 AT4G09920 33.533 167 100 5 20 183 2 160 2.04e-15 77.0
MS.gene64992.t1 AT3G50710 25.813 461 282 14 20 455 2 427 2.90e-15 78.2
MS.gene64992.t1 AT5G56420 24.724 453 288 17 20 455 6 422 4.07e-15 77.4
MS.gene64992.t1 AT5G56420 24.724 453 288 17 20 455 6 422 4.07e-15 77.4
MS.gene64992.t1 AT5G56420 24.724 453 288 17 20 455 6 422 4.07e-15 77.4
MS.gene64992.t1 AT5G56440 35.106 188 112 4 20 204 2 182 1.22e-14 76.3
MS.gene64992.t1 AT5G53635 25.275 455 287 15 22 454 2 425 1.60e-14 75.9
MS.gene64992.t1 AT4G13965 27.391 460 260 19 19 452 9 420 1.06e-13 73.2
MS.gene64992.t1 AT4G26340 26.915 457 274 17 20 455 2 419 1.27e-13 72.8
MS.gene64992.t1 AT1G16930 29.756 205 126 7 8 206 3 195 2.16e-12 69.3
MS.gene64992.t1 AT1G16930 29.756 205 126 7 8 206 3 195 2.16e-12 69.3
MS.gene64992.t1 AT1G16930 29.756 205 126 7 8 206 3 195 2.16e-12 69.3
MS.gene64992.t1 AT3G59190 28.571 175 105 4 20 183 12 177 2.77e-12 69.3
MS.gene64992.t1 AT5G60610 25.000 420 257 19 20 419 2 383 3.16e-12 68.6
MS.gene64992.t1 AT5G60610 25.000 420 257 19 20 419 2 383 3.16e-12 68.6
MS.gene64992.t1 AT3G59190 28.571 175 105 4 20 183 12 177 4.06e-12 68.2
MS.gene64992.t1 AT5G53840 23.864 440 253 16 20 425 18 409 9.30e-12 67.4
MS.gene64992.t1 AT3G59200 29.000 200 114 7 20 204 7 193 1.34e-11 66.2
MS.gene64992.t1 AT4G14103 30.928 194 116 5 20 204 8 192 1.64e-11 66.6
MS.gene64992.t1 AT4G14103 30.729 192 119 4 20 204 8 192 1.68e-11 66.2
MS.gene64992.t1 AT4G14103 30.729 192 119 4 20 204 8 192 1.68e-11 66.2
MS.gene64992.t1 AT3G59200 29.000 200 114 7 20 204 7 193 2.78e-11 65.9
MS.gene64992.t1 AT4G13985 31.658 199 118 9 19 206 18 209 5.36e-11 65.1
MS.gene64992.t1 AT3G52680 31.795 195 115 6 20 207 21 204 6.93e-11 64.7
MS.gene64992.t1 AT1G48400 29.897 194 119 6 20 204 10 195 7.25e-11 64.7
MS.gene64992.t1 AT1G48400 29.897 194 119 6 20 204 10 195 9.01e-11 64.3

Find 111 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATAGTTTACCTTCTATT+TGG 0.229961 8.4:+39626316 MS.gene64992:CDS
TGTTGGCAACTTTCACAGTA+TGG 0.256170 8.4:+39626516 MS.gene64992:CDS
GAAATTGTCATTGGCCGATT+TGG 0.324884 8.4:-39626437 None:intergenic
CAACTTAAATTTGACGTTGA+TGG 0.335217 8.4:+39627323 MS.gene64992:CDS
CTCGCCGGTGGCGCCACCTC+TGG 0.341008 8.4:+39625790 MS.gene64992:CDS
AAATAATGAATGTATTAGAT+TGG 0.376850 8.4:+39626489 MS.gene64992:CDS
TGATGTAAACGGCTAAATTC+AGG 0.379198 8.4:-39626811 None:intergenic
GGAAGGTGGAATTAAAGCTT+TGG 0.380976 8.4:-39626398 None:intergenic
CTCGGATGAGGTTGAGAATA+GGG 0.388430 8.4:-39626588 None:intergenic
GACGTTGATGGGTCTCATTA+AGG 0.393555 8.4:+39627335 MS.gene64992:CDS
CTGACTTATGTTCACATAGA+AGG 0.406124 8.4:+39627137 MS.gene64992:CDS
AACTTAAATTTGACGTTGAT+GGG 0.410278 8.4:+39627324 MS.gene64992:CDS
GTAAAGGAGGTTGGAGCAAT+TGG 0.413248 8.4:-39626087 None:intergenic
TATGAATGTCGCGGAATTTG+CGG 0.415114 8.4:-39625905 None:intergenic
TAGATTCACATTCGTTCAAA+AGG 0.416585 8.4:+39625850 MS.gene64992:CDS
GAGATAGAGTTGTTGAAGGT+GGG 0.425273 8.4:-39626018 None:intergenic
ACTTGGGACTGCCAAATAGA+AGG 0.427636 8.4:-39626327 None:intergenic
GACTGCAGAAGTTGAATATC+TGG 0.436258 8.4:-39625821 None:intergenic
CGATTTGGAGCTCTCGGAGC+TGG 0.442706 8.4:-39626422 None:intergenic
AATAATGAATGTATTAGATT+GGG 0.455243 8.4:+39626490 MS.gene64992:CDS
TGTATTAGATTGGGCATTGT+TGG 0.462655 8.4:+39626499 MS.gene64992:CDS
CCTTCCAGAGGTGGCGCCAC+CGG 0.463852 8.4:-39625794 None:intergenic
CATCATAGCGACGGAGGTTG+TGG 0.470605 8.4:-39625760 None:intergenic
AACCCGCAAAACTTCCAACT+TGG 0.473471 8.4:-39626344 None:intergenic
GCTCCAACCTCCTTTACCTC+AGG 0.478577 8.4:+39626093 MS.gene64992:CDS
TATACATCATAGCAGTCAGC+TGG 0.478695 8.4:-39626643 None:intergenic
TCTCGGATGAGGTTGAGAAT+AGG 0.481952 8.4:-39626589 None:intergenic
GAAGGTGGAATTAAAGCTTT+GGG 0.484659 8.4:-39626397 None:intergenic
GCAATTATAACCTGAGGTAA+AGG 0.484740 8.4:-39626103 None:intergenic
CATCAACGTCAAATTTAAGT+TGG 0.486323 8.4:-39627322 None:intergenic
GTTGGCAACTTTCACAGTAT+GGG 0.487162 8.4:+39626517 MS.gene64992:CDS
TGTAGGTTCTTATAGTATAG+AGG 0.487842 8.4:+39626222 MS.gene64992:CDS
CTCAACCTCATCCGAGATGA+TGG 0.488519 8.4:+39626595 MS.gene64992:CDS
ACGTGGATCCTTGCTGCCAT+TGG 0.491503 8.4:+39625993 MS.gene64992:CDS
ATGTTTCGACTCAAATCTGC+TGG 0.494251 8.4:+39626852 MS.gene64992:CDS
CATAAGTTCCATCTCATACT+CGG 0.496407 8.4:+39625924 MS.gene64992:CDS
CGGCCAATGACAATTTCACT+TGG 0.496462 8.4:+39626443 MS.gene64992:CDS
CTTCTATGTGAACATAAGTC+AGG 0.496958 8.4:-39627136 None:intergenic
GCCATTGAGAACATGGCAGC+TGG 0.499987 8.4:+39627079 MS.gene64992:CDS
TGAACTTCAGATGATGTAAA+CGG 0.503521 8.4:-39626822 None:intergenic
GACAAATTCACTTTCAGTCA+AGG 0.506419 8.4:-39626563 None:intergenic
CAATTGGTCAAGAGGGAAGG+AGG 0.511905 8.4:-39626071 None:intergenic
TGCCATGTTCTCAATGGCAG+TGG 0.512316 8.4:-39627074 None:intergenic
TTCTGCCATCATCTCGGATG+AGG 0.513011 8.4:-39626600 None:intergenic
ATTGGCCGATTTGGAGCTCT+CGG 0.514913 8.4:-39626428 None:intergenic
TTGTTGAAGGTGGGGTCCAA+TGG 0.515637 8.4:-39626009 None:intergenic
TCCAGCTGCCATGTTCTCAA+TGG 0.515812 8.4:-39627080 None:intergenic
TTGGCAACTTTCACAGTATG+GGG 0.521026 8.4:+39626518 MS.gene64992:CDS
GTTCACATAGAAGGATATCA+AGG 0.522561 8.4:+39627146 MS.gene64992:CDS
TAACCTGAGGTAAAGGAGGT+TGG 0.524882 8.4:-39626096 None:intergenic
AGTCCAAGTGAAATTGTCAT+TGG 0.525257 8.4:-39626446 None:intergenic
TGTAGAGATAGAGTTGTTGA+AGG 0.526137 8.4:-39626022 None:intergenic
CAGCTCCGAGAGCTCCAAAT+CGG 0.527982 8.4:+39626423 MS.gene64992:CDS
GAGCTCTCGGAGCTGGAGGA+AGG 0.528347 8.4:-39626415 None:intergenic
AGAGATAGAGTTGTTGAAGG+TGG 0.529187 8.4:-39626019 None:intergenic
AAGATTAACGAATATATCAA+AGG 0.534259 8.4:+39627286 MS.gene64992:CDS
TGATAGTTCAATAGACAAAA+CGG 0.539124 8.4:+39627244 MS.gene64992:CDS
GGAGGTTGTGGTAGGGAGGA+AGG 0.546045 8.4:-39625748 None:intergenic
CCGGTGGCGCCACCTCTGGA+AGG 0.547084 8.4:+39625794 MS.gene64992:CDS
AGATTAACGAATATATCAAA+GGG 0.548268 8.4:+39627287 MS.gene64992:CDS
TCTATTTGGCAGTCCCAAGT+TGG 0.550189 8.4:+39626330 MS.gene64992:CDS
AGCAGATTTGAGTCGAAACA+TGG 0.553943 8.4:-39626850 None:intergenic
TGTGGCATTGCTCAAGAACA+TGG 0.554101 8.4:-39626876 None:intergenic
CTCTCGGAGCTGGAGGAAGG+TGG 0.556854 8.4:-39626412 None:intergenic
GAGATGAAAGCTGTCACAAA+CGG 0.557077 8.4:-39625958 None:intergenic
AACCACTGCCATTGAGAACA+TGG 0.558537 8.4:+39627072 MS.gene64992:CDS
ATATGACACAGGATCGAGTC+CGG 0.559164 8.4:-39625720 None:intergenic
AACCTAATCGACGATGGCAA+CGG 0.562160 8.4:+39625632 None:intergenic
TTCAGGATAATTTAGAACAC+GGG 0.564152 8.4:-39626794 None:intergenic
AATTGGTCAAGAGGGAAGGA+GGG 0.570036 8.4:-39626070 None:intergenic
ACCCGCAAAACTTCCAACTT+GGG 0.570628 8.4:-39626343 None:intergenic
TTCAATAGACAAAACGGACA+TGG 0.570795 8.4:+39627250 MS.gene64992:CDS
GGACAGATTGAGCAGTCTAC+CGG 0.570908 8.4:+39625701 MS.gene64992:CDS
ATAGCGACGGAGGTTGTGGT+AGG 0.574834 8.4:-39625756 None:intergenic
AGATAGAGTTGTTGAAGGTG+GGG 0.580102 8.4:-39626017 None:intergenic
GATTAAAGCCGAGTATGAGA+TGG 0.586475 8.4:-39625932 None:intergenic
AGAGACAGCACTTTGAGCAA+TGG 0.588422 8.4:-39626268 None:intergenic
ATTCAGTTCTGCCATCATCT+CGG 0.593799 8.4:-39626606 None:intergenic
GAGCAATTGGTCAAGAGGGA+AGG 0.596231 8.4:-39626074 None:intergenic
GGTTGGAGCAATTGGTCAAG+AGG 0.598188 8.4:-39626079 None:intergenic
ATCTCGCTTGTATTCGTACG+TGG 0.599453 8.4:+39625976 MS.gene64992:CDS
GTTGCGTCATTCAACATCAA+AGG 0.603041 8.4:+39626672 MS.gene64992:CDS
CTATGTGAACATAAGTCAGG+TGG 0.604289 8.4:-39627133 None:intergenic
GATGGCAACGGAGTCAAGCA+TGG 0.614265 8.4:+39625644 None:intergenic
TAGCGACGGAGGTTGTGGTA+GGG 0.614525 8.4:-39625755 None:intergenic
GAATGTCGCGGAATTTGCGG+AGG 0.615868 8.4:-39625902 None:intergenic
TAATTTAGAACACGGGCATG+AGG 0.618358 8.4:-39626787 None:intergenic
GTTGGAGCAATTGGTCAAGA+GGG 0.618704 8.4:-39626078 None:intergenic
CGACGGAGGTTGTGGTAGGG+AGG 0.621858 8.4:-39625752 None:intergenic
GGTGGGGTCCAATGGCAGCA+AGG 0.624675 8.4:-39626001 None:intergenic
ATTATAACCTGAGGTAAAGG+AGG 0.629004 8.4:-39626100 None:intergenic
ATTCAGGATAATTTAGAACA+CGG 0.629666 8.4:-39626795 None:intergenic
TGATGAGAGTCTCTCGCCGG+TGG 0.630142 8.4:+39625778 MS.gene64992:CDS
TCTGGAGATCCTTCCAGAGG+TGG 0.631588 8.4:-39625803 None:intergenic
TTTGAGCAATGGAAAATGAA+CGG 0.634422 8.4:-39626257 None:intergenic
TTTGGAGCTCTCGGAGCTGG+AGG 0.636682 8.4:-39626419 None:intergenic
TATGTGAACATAAGTCAGGT+GGG 0.640734 8.4:-39627132 None:intergenic
CTATGATGAGAGTCTCTCGC+CGG 0.640993 8.4:+39625775 MS.gene64992:CDS
TCTCATAATTCAGAGCAACA+AGG 0.643040 8.4:+39626909 MS.gene64992:CDS
ATACATCATAGCAGTCAGCT+GGG 0.647711 8.4:-39626642 None:intergenic
GGAAGGACATGATATGACAC+AGG 0.667460 8.4:-39625731 None:intergenic
AGAGACTCTCATCATAGCGA+CGG 0.669339 8.4:-39625769 None:intergenic
GATGGAACTTATGAATGTCG+CGG 0.686296 8.4:-39625914 None:intergenic
GACTCTCATCATAGCGACGG+AGG 0.689212 8.4:-39625766 None:intergenic
AATGTCGCGGAATTTGCGGA+GGG 0.690292 8.4:-39625901 None:intergenic
TGGCAACTTTCACAGTATGG+GGG 0.693092 8.4:+39626519 MS.gene64992:CDS
TTATGAGAACTTGAAGCATG+TGG 0.695487 8.4:-39626894 None:intergenic
TGCAGAAGAAGACAACGTCA+CGG 0.699654 8.4:+39625680 MS.gene64992:CDS
ACTGCTATGATGTATATCTG+CGG 0.701238 8.4:+39626650 MS.gene64992:CDS
ATATCTGGAGATCCTTCCAG+AGG 0.715901 8.4:-39625806 None:intergenic
CATCATAGCAGTCAGCTGGG+AGG 0.736987 8.4:-39626639 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATGAATGTATTAGAT+TGG + chr8.4:39626489-39626508 MS.gene64992:CDS 15.0%
!! AATAATGAATGTATTAGATT+GGG + chr8.4:39626490-39626509 MS.gene64992:CDS 15.0%
!! AAGATTAACGAATATATCAA+AGG + chr8.4:39627286-39627305 MS.gene64992:CDS 20.0%
!! ACATACATACATGTATATAT+AGG - chr8.4:39626977-39626996 None:intergenic 20.0%
!! AGATTAACGAATATATCAAA+GGG + chr8.4:39627287-39627306 MS.gene64992:CDS 20.0%
!! AGTTTAATACAAACAATCTT+TGG + chr8.4:39626947-39626966 MS.gene64992:intron 20.0%
!! ATGGTTTATATGTATATCTA+CGG + chr8.4:39626710-39626729 MS.gene64992:intron 20.0%
!! GTCATTACTCAAAAATATAT+TGG - chr8.4:39627025-39627044 None:intergenic 20.0%
! AACTTAAATTTGACGTTGAT+GGG + chr8.4:39627324-39627343 MS.gene64992:CDS 25.0%
! GAAATAGTTTACCTTCTATT+TGG + chr8.4:39626316-39626335 MS.gene64992:CDS 25.0%
! TAAAAAGCAATTATAACCTG+AGG - chr8.4:39626112-39626131 None:intergenic 25.0%
! TGATAGTTCAATAGACAAAA+CGG + chr8.4:39627244-39627263 MS.gene64992:CDS 25.0%
! TTATATGTATATCTACGGTT+TGG + chr8.4:39626715-39626734 MS.gene64992:intron 25.0%
! TTTCAAAAGACATACATGTT+AGG - chr8.4:39626195-39626214 None:intergenic 25.0%
!! ATTCAGGATAATTTAGAACA+CGG - chr8.4:39626798-39626817 None:intergenic 25.0%
!! TTTTAGACTAACAATTTCTG+CGG - chr8.4:39626296-39626315 None:intergenic 25.0%
!!! AAGGTGATGTTTTATTCTTA+TGG + chr8.4:39626691-39626710 MS.gene64992:intron 25.0%
!!! CATTTGCTGAATATATTTTG+CGG + chr8.4:39627186-39627205 MS.gene64992:CDS 25.0%
!!! TATATTTTTGAGTAATGACC+TGG + chr8.4:39627026-39627045 MS.gene64992:intron 25.0%
CAACTTAAATTTGACGTTGA+TGG + chr8.4:39627323-39627342 MS.gene64992:CDS 30.0%
CATCAACGTCAAATTTAAGT+TGG - chr8.4:39627325-39627344 None:intergenic 30.0%
GAAATGTTAGAAGTGAGTTT+GGG - chr8.4:39626137-39626156 None:intergenic 30.0%
TAGATTCACATTCGTTCAAA+AGG + chr8.4:39625850-39625869 MS.gene64992:CDS 30.0%
TGAAATGTTAGAAGTGAGTT+TGG - chr8.4:39626138-39626157 None:intergenic 30.0%
TGAACTTCAGATGATGTAAA+CGG - chr8.4:39626825-39626844 None:intergenic 30.0%
TGTAGGTTCTTATAGTATAG+AGG + chr8.4:39626222-39626241 MS.gene64992:CDS 30.0%
TTTGAGCAATGGAAAATGAA+CGG - chr8.4:39626260-39626279 None:intergenic 30.0%
! TTCAGGATAATTTAGAACAC+GGG - chr8.4:39626797-39626816 None:intergenic 30.0%
!! TCTTTTGAAAACAGTCTTGT+AGG + chr8.4:39626205-39626224 MS.gene64992:intron 30.0%
ACTGCTATGATGTATATCTG+CGG + chr8.4:39626650-39626669 MS.gene64992:CDS 35.0%
AGTCCAAGTGAAATTGTCAT+TGG - chr8.4:39626449-39626468 None:intergenic 35.0%
ATTATAACCTGAGGTAAAGG+AGG - chr8.4:39626103-39626122 None:intergenic 35.0%
CATAAGTTCCATCTCATACT+CGG + chr8.4:39625924-39625943 MS.gene64992:CDS 35.0%
CTGACTTATGTTCACATAGA+AGG + chr8.4:39627137-39627156 MS.gene64992:CDS 35.0%
CTTCTATGTGAACATAAGTC+AGG - chr8.4:39627139-39627158 None:intergenic 35.0%
GAAGGTGGAATTAAAGCTTT+GGG - chr8.4:39626400-39626419 None:intergenic 35.0%
GACAAATTCACTTTCAGTCA+AGG - chr8.4:39626566-39626585 None:intergenic 35.0%
GCAATTATAACCTGAGGTAA+AGG - chr8.4:39626106-39626125 None:intergenic 35.0%
GTTCACATAGAAGGATATCA+AGG + chr8.4:39627146-39627165 MS.gene64992:CDS 35.0%
TATGTGAACATAAGTCAGGT+GGG - chr8.4:39627135-39627154 None:intergenic 35.0%
TCTCATAATTCAGAGCAACA+AGG + chr8.4:39626909-39626928 MS.gene64992:CDS 35.0%
TGATGTAAACGGCTAAATTC+AGG - chr8.4:39626814-39626833 None:intergenic 35.0%
TGTAGAGATAGAGTTGTTGA+AGG - chr8.4:39626025-39626044 None:intergenic 35.0%
TTATGAGAACTTGAAGCATG+TGG - chr8.4:39626897-39626916 None:intergenic 35.0%
TTCAATAGACAAAACGGACA+TGG + chr8.4:39627250-39627269 MS.gene64992:CDS 35.0%
! GCTGAATATATTTTGCGGAA+TGG + chr8.4:39627191-39627210 MS.gene64992:CDS 35.0%
! TGTATTAGATTGGGCATTGT+TGG + chr8.4:39626499-39626518 MS.gene64992:CDS 35.0%
AGAGATAGAGTTGTTGAAGG+TGG - chr8.4:39626022-39626041 None:intergenic 40.0%
AGATAGAGTTGTTGAAGGTG+GGG - chr8.4:39626020-39626039 None:intergenic 40.0%
AGCAGATTTGAGTCGAAACA+TGG - chr8.4:39626853-39626872 None:intergenic 40.0%
ATACATCATAGCAGTCAGCT+GGG - chr8.4:39626645-39626664 None:intergenic 40.0%
ATGTTTCGACTCAAATCTGC+TGG + chr8.4:39626852-39626871 MS.gene64992:CDS 40.0%
ATTCAGTTCTGCCATCATCT+CGG - chr8.4:39626609-39626628 None:intergenic 40.0%
CTATGTGAACATAAGTCAGG+TGG - chr8.4:39627136-39627155 None:intergenic 40.0%
GAAATTGTCATTGGCCGATT+TGG - chr8.4:39626440-39626459 None:intergenic 40.0%
GACTGCAGAAGTTGAATATC+TGG - chr8.4:39625824-39625843 None:intergenic 40.0%
GAGATAGAGTTGTTGAAGGT+GGG - chr8.4:39626021-39626040 None:intergenic 40.0%
GAGATGAAAGCTGTCACAAA+CGG - chr8.4:39625961-39625980 None:intergenic 40.0%
GATGGAACTTATGAATGTCG+CGG - chr8.4:39625917-39625936 None:intergenic 40.0%
GATTAAAGCCGAGTATGAGA+TGG - chr8.4:39625935-39625954 None:intergenic 40.0%
GGAAGGTGGAATTAAAGCTT+TGG - chr8.4:39626401-39626420 None:intergenic 40.0%
GTTGCGTCATTCAACATCAA+AGG + chr8.4:39626672-39626691 MS.gene64992:CDS 40.0%
GTTGGCAACTTTCACAGTAT+GGG + chr8.4:39626517-39626536 MS.gene64992:CDS 40.0%
TATACATCATAGCAGTCAGC+TGG - chr8.4:39626646-39626665 None:intergenic 40.0%
TATGAATGTCGCGGAATTTG+CGG - chr8.4:39625908-39625927 None:intergenic 40.0%
TGTTGGCAACTTTCACAGTA+TGG + chr8.4:39626516-39626535 MS.gene64992:CDS 40.0%
TTGGCAACTTTCACAGTATG+GGG + chr8.4:39626518-39626537 MS.gene64992:CDS 40.0%
! TAATTTAGAACACGGGCATG+AGG - chr8.4:39626790-39626809 None:intergenic 40.0%
AACCACTGCCATTGAGAACA+TGG + chr8.4:39627072-39627091 MS.gene64992:CDS 45.0%
AACCCGCAAAACTTCCAACT+TGG - chr8.4:39626347-39626366 None:intergenic 45.0%
AATGACCTGGTTTCCCTTGT+TGG + chr8.4:39627039-39627058 MS.gene64992:intron 45.0%
AATTGGTCAAGAGGGAAGGA+GGG - chr8.4:39626073-39626092 None:intergenic 45.0%
ACCCGCAAAACTTCCAACTT+GGG - chr8.4:39626346-39626365 None:intergenic 45.0%
ACTTGGGACTGCCAAATAGA+AGG - chr8.4:39626330-39626349 None:intergenic 45.0%
AGAGACAGCACTTTGAGCAA+TGG - chr8.4:39626271-39626290 None:intergenic 45.0%
AGAGACTCTCATCATAGCGA+CGG - chr8.4:39625772-39625791 None:intergenic 45.0%
ATATCTGGAGATCCTTCCAG+AGG - chr8.4:39625809-39625828 None:intergenic 45.0%
ATATGACACAGGATCGAGTC+CGG - chr8.4:39625723-39625742 None:intergenic 45.0%
ATCTCGCTTGTATTCGTACG+TGG + chr8.4:39625976-39625995 MS.gene64992:CDS 45.0%
CGGCCAATGACAATTTCACT+TGG + chr8.4:39626443-39626462 MS.gene64992:CDS 45.0%
CTCGGATGAGGTTGAGAATA+GGG - chr8.4:39626591-39626610 None:intergenic 45.0%
GGAAGGACATGATATGACAC+AGG - chr8.4:39625734-39625753 None:intergenic 45.0%
GTAAAGGAGGTTGGAGCAAT+TGG - chr8.4:39626090-39626109 None:intergenic 45.0%
GTTGGAGCAATTGGTCAAGA+GGG - chr8.4:39626081-39626100 None:intergenic 45.0%
TAACCTGAGGTAAAGGAGGT+TGG - chr8.4:39626099-39626118 None:intergenic 45.0%
TCTATTTGGCAGTCCCAAGT+TGG + chr8.4:39626330-39626349 MS.gene64992:CDS 45.0%
TCTCGGATGAGGTTGAGAAT+AGG - chr8.4:39626592-39626611 None:intergenic 45.0%
TGCAGAAGAAGACAACGTCA+CGG + chr8.4:39625680-39625699 MS.gene64992:CDS 45.0%
TGGCAACTTTCACAGTATGG+GGG + chr8.4:39626519-39626538 MS.gene64992:CDS 45.0%
TGTGGCATTGCTCAAGAACA+TGG - chr8.4:39626879-39626898 None:intergenic 45.0%
TTGTTATCGTGTACCTGCAG+TGG + chr8.4:39626761-39626780 MS.gene64992:intron 45.0%
!! GTCCCAAGTTGGAAGTTTTG+CGG + chr8.4:39626341-39626360 MS.gene64992:CDS 45.0%
!! TCCCAAGTTGGAAGTTTTGC+GGG + chr8.4:39626342-39626361 MS.gene64992:CDS 45.0%
AATGTCGCGGAATTTGCGGA+GGG - chr8.4:39625904-39625923 None:intergenic 50.0%
CAATTGGTCAAGAGGGAAGG+AGG - chr8.4:39626074-39626093 None:intergenic 50.0%
CTATGATGAGAGTCTCTCGC+CGG + chr8.4:39625775-39625794 MS.gene64992:CDS 50.0%
CTCAACCTCATCCGAGATGA+TGG + chr8.4:39626595-39626614 MS.gene64992:CDS 50.0%
GAGCAATTGGTCAAGAGGGA+AGG - chr8.4:39626077-39626096 None:intergenic 50.0%
GGACAGATTGAGCAGTCTAC+CGG + chr8.4:39625701-39625720 MS.gene64992:CDS 50.0%
GGTTGGAGCAATTGGTCAAG+AGG - chr8.4:39626082-39626101 None:intergenic 50.0%
GTTCCTCCTACCACCAACAA+GGG - chr8.4:39627055-39627074 None:intergenic 50.0%
TACCACCAACAAGGGAAACC+AGG - chr8.4:39627047-39627066 None:intergenic 50.0%
TCCAGCTGCCATGTTCTCAA+TGG - chr8.4:39627083-39627102 None:intergenic 50.0%
TGCCATGTTCTCAATGGCAG+TGG - chr8.4:39627077-39627096 None:intergenic 50.0%
TTCTGCCATCATCTCGGATG+AGG - chr8.4:39626603-39626622 None:intergenic 50.0%
TTGTTGAAGGTGGGGTCCAA+TGG - chr8.4:39626012-39626031 None:intergenic 50.0%
!! ATTGGCCGATTTGGAGCTCT+CGG - chr8.4:39626431-39626450 None:intergenic 50.0%
!! TGGTTTCCCTTGTTGGTGGT+AGG + chr8.4:39627046-39627065 MS.gene64992:intron 50.0%
!! TTTCCCTTGTTGGTGGTAGG+AGG + chr8.4:39627049-39627068 MS.gene64992:intron 50.0%
ACGTGGATCCTTGCTGCCAT+TGG + chr8.4:39625993-39626012 MS.gene64992:CDS 55.0%
ATAGCGACGGAGGTTGTGGT+AGG - chr8.4:39625759-39625778 None:intergenic 55.0%
CAGCTCCGAGAGCTCCAAAT+CGG + chr8.4:39626423-39626442 MS.gene64992:CDS 55.0%
CATCATAGCAGTCAGCTGGG+AGG - chr8.4:39626642-39626661 None:intergenic 55.0%
CATCATAGCGACGGAGGTTG+TGG - chr8.4:39625763-39625782 None:intergenic 55.0%
GAATGTCGCGGAATTTGCGG+AGG - chr8.4:39625905-39625924 None:intergenic 55.0%
GACCTGGTTTCCCTTGTTGG+TGG + chr8.4:39627042-39627061 MS.gene64992:intron 55.0%
GACTCTCATCATAGCGACGG+AGG - chr8.4:39625769-39625788 None:intergenic 55.0%
GCCATTGAGAACATGGCAGC+TGG + chr8.4:39627079-39627098 MS.gene64992:CDS 55.0%
GCTCCAACCTCCTTTACCTC+AGG + chr8.4:39626093-39626112 MS.gene64992:CDS 55.0%
GGTTCCTCCTACCACCAACA+AGG - chr8.4:39627056-39627075 None:intergenic 55.0%
TAGCGACGGAGGTTGTGGTA+GGG - chr8.4:39625758-39625777 None:intergenic 55.0%
TCTGGAGATCCTTCCAGAGG+TGG - chr8.4:39625806-39625825 None:intergenic 55.0%
GGAGGTTGTGGTAGGGAGGA+AGG - chr8.4:39625751-39625770 None:intergenic 60.0%
TGATGAGAGTCTCTCGCCGG+TGG + chr8.4:39625778-39625797 MS.gene64992:CDS 60.0%
! CGATTTGGAGCTCTCGGAGC+TGG - chr8.4:39626425-39626444 None:intergenic 60.0%
! TTTGGAGCTCTCGGAGCTGG+AGG - chr8.4:39626422-39626441 None:intergenic 60.0%
CGACGGAGGTTGTGGTAGGG+AGG - chr8.4:39625755-39625774 None:intergenic 65.0%
CTCTCGGAGCTGGAGGAAGG+TGG - chr8.4:39626415-39626434 None:intergenic 65.0%
GAGCTCTCGGAGCTGGAGGA+AGG - chr8.4:39626418-39626437 None:intergenic 65.0%
GGTGGGGTCCAATGGCAGCA+AGG - chr8.4:39626004-39626023 None:intergenic 65.0%
CCTTCCAGAGGTGGCGCCAC+CGG - chr8.4:39625797-39625816 None:intergenic 70.0%
GGGCATGAGGTCGCCACTGC+AGG - chr8.4:39626777-39626796 None:intergenic 70.0%
CCGGTGGCGCCACCTCTGGA+AGG + chr8.4:39625794-39625813 MS.gene64992:CDS 75.0%
! CTCGCCGGTGGCGCCACCTC+TGG + chr8.4:39625790-39625809 MS.gene64992:CDS 80.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 39625645 39627355 39625645 ID=MS.gene64992
chr8.4 mRNA 39625645 39627355 39625645 ID=MS.gene64992.t1;Parent=MS.gene64992
chr8.4 exon 39625645 39626114 39625645 ID=MS.gene64992.t1.exon1;Parent=MS.gene64992.t1
chr8.4 CDS 39625645 39626114 39625645 ID=cds.MS.gene64992.t1;Parent=MS.gene64992.t1
chr8.4 exon 39626219 39626693 39626219 ID=MS.gene64992.t1.exon2;Parent=MS.gene64992.t1
chr8.4 CDS 39626219 39626693 39626219 ID=cds.MS.gene64992.t1;Parent=MS.gene64992.t1
chr8.4 exon 39626781 39626930 39626781 ID=MS.gene64992.t1.exon3;Parent=MS.gene64992.t1
chr8.4 CDS 39626781 39626930 39626781 ID=cds.MS.gene64992.t1;Parent=MS.gene64992.t1
chr8.4 exon 39627068 39627355 39627068 ID=MS.gene64992.t1.exon4;Parent=MS.gene64992.t1
chr8.4 CDS 39627068 39627355 39627068 ID=cds.MS.gene64992.t1;Parent=MS.gene64992.t1
Gene Sequence

>MS.gene64992

ATGGCAACGGAGTCAAGCATGGAACGATCGCTTGTTGCAGAAGAAGACAACGTCACGGACAGATTGAGCAGTCTACCGGACTCGATCCTGTGTCATATCATGTCCTTCCTCCCTACCACAACCTCCGTCGCTATGATGAGAGTCTCTCGCCGGTGGCGCCACCTCTGGAAGGATCTCCAGATATTCAACTTCTGCAGTCGCAATCTAGATTCACATTCGTTCAAAAGGTTTTTATTATTCGTGAACGCTGTACTTACCCTCCGCAAATTCCGCGACATTCATAAGTTCCATCTCATACTCGGCTTTAATCGATCCGTTTGTGACAGCTTTCATCTCGCTTGTATTCGTACGTGGATCCTTGCTGCCATTGGACCCCACCTTCAACAACTCTATCTCTACATTGTCAATGCTGCTGTTGTAGTTCTCCCTCCTTCCCTCTTGACCAATTGCTCCAACCTCCTTTACCTCAGGTTATAATTGCTTTTTATGCCCAAACTCACTTCTAACATTTCATCTCTTGTTTTTTTTACTTTTTTTTTTGTTCTTTCCTAACATGTATGTCTTTTGAAAACAGTCTTGTAGGTTCTTATAGTATAGAGGTAAAACAGTCTTCCGTTCATTTTCCATTGCTCAAAGTGCTGTCTCTCGCCGCAGAAATTGTTAGTCTAAAAGAAATAGTTTACCTTCTATTTGGCAGTCCCAAGTTGGAAGTTTTGCGGGTTTACTTTTACTTTATCTCTGAAGAACTTGTGCCCAAAGCTTTAATTCCACCTTCCTCCAGCTCCGAGAGCTCCAAATCGGCCAATGACAATTTCACTTGGACTTACTTTAAAATAGTTAACACAAATAATGAATGTATTAGATTGGGCATTGTTGGCAACTTTCACAGTATGGGGGAAGCATTTCTTGATGTCTTTTCCTTGACTGAAAGTGAATTTGTCGACCCTATTCTCAACCTCATCCGAGATGATGGCAGAACTGAATTTTATAAAAACCTCCCAGCTGACTGCTATGATGTATATCTGCGGTTGCGTCATTCAACATCAAAGGTGATGTTTTATTCTTATGGTTTATATGTATATCTACGGTTTGGTACTAATAGTGCGTCATCACTTGTTGTTATCGTGTACCTGCAGTGGCGACCTCATGCCCGTGTTCTAAATTATCCTGAATTTAGCCGTTTACATCATCTGAAGTTCATTCTTCCATGTTTCGACTCAAATCTGCTGGTCCATGTTCTTGAGCAATGCCACATGCTTCAAGTTCTCATAATTCAGAGCAACAAGGTTTGTTGACTTCTCAAGTTTAATACAAACAATCTTTGGTCTGCCTATATATACATGTATGTATGTATGTATGTATGTATCTATCTGTACACCAATATATTTTTGAGTAATGACCTGGTTTCCCTTGTTGGTGGTAGGAGGAACCACTGCCATTGAGAACATGGCAGCTGGAGTCAACAACAGTTTTTGAATGTCTAAAATCCCACCTGACTTATGTTCACATAGAAGGATATCAAGGACTTGAAGATGAGTTCACATTTGCTGAATATATTTTGCGGAATGGACTTCTTTTGAAGACGATGCTTATTTTCGTTGATAGTTCAATAGACAAAACGGACATGGATTGTTCTCTCAAAAGATTAACGAATATATCAAAGGGCTCTGTTGCTTGCCAACTTAAATTTGACGTTGATGGGTCTCATTAA
Protein sequence

>MS.gene64992.t1

MATESSMERSLVAEEDNVTDRLSSLPDSILCHIMSFLPTTTSVAMMRVSRRWRHLWKDLQIFNFCSRNLDSHSFKRFLLFVNAVLTLRKFRDIHKFHLILGFNRSVCDSFHLACIRTWILAAIGPHLQQLYLYIVNAAVVVLPPSLLTNCSNLLYLSLVGSYSIEVKQSSVHFPLLKVLSLAAEIVSLKEIVYLLFGSPKLEVLRVYFYFISEELVPKALIPPSSSSESSKSANDNFTWTYFKIVNTNNECIRLGIVGNFHSMGEAFLDVFSLTESEFVDPILNLIRDDGRTEFYKNLPADCYDVYLRLRHSTSKWRPHARVLNYPEFSRLHHLKFILPCFDSNLLVHVLEQCHMLQVLIIQSNKEEPLPLRTWQLESTTVFECLKSHLTYVHIEGYQGLEDEFTFAEYILRNGLLLKTMLIFVDSSIDKTDMDCSLKRLTNISKGSVACQLKFDVDGSH