AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053311

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053311.t1 MTR_8g469020 61.504 452 125 4 21 424 6 456 0.0 538
MS.gene053311.t1 MTR_8g058965 63.261 460 120 4 11 423 3 460 0.0 533
MS.gene053311.t1 MTR_4g050250 63.981 422 93 7 1 365 1 420 6.43e-175 496
MS.gene053311.t1 MTR_8g062010 56.293 437 145 9 22 423 9 434 7.00e-154 442
MS.gene053311.t1 MTR_8g070950 56.081 444 141 10 19 424 12 439 6.14e-148 427
MS.gene053311.t1 MTR_8g062310 51.648 455 167 9 1 424 1 433 2.64e-143 416
MS.gene053311.t1 MTR_8g062080 51.204 457 174 11 4 423 5 449 2.56e-141 411
MS.gene053311.t1 MTR_8g062300 46.817 487 172 13 8 423 11 481 1.62e-124 369
MS.gene053311.t1 MTR_2g083840 43.326 457 205 9 8 419 10 457 5.21e-97 298
MS.gene053311.t1 MTR_8g468810 41.845 466 213 12 6 419 4 463 4.71e-96 296
MS.gene053311.t1 MTR_8g467790 47.273 385 121 13 22 363 148 493 8.55e-95 294
MS.gene053311.t1 MTR_8g058970 40.862 487 202 16 6 419 4 477 1.96e-90 282
MS.gene053311.t1 MTR_8g062270 41.538 455 197 15 19 418 13 453 6.45e-85 267
MS.gene053311.t1 MTR_4g032730 38.478 473 218 11 10 418 3 466 1.36e-82 261
MS.gene053311.t1 MTR_8g467800 40.977 471 211 13 6 424 3 458 3.05e-81 258
MS.gene053311.t1 MTR_8g014260 39.738 458 221 17 6 419 5 451 1.97e-80 255
MS.gene053311.t1 MTR_5g092960 37.908 459 215 13 18 418 15 461 1.54e-77 248
MS.gene053311.t1 MTR_8g468840 39.865 444 210 12 22 417 14 448 1.79e-77 248
MS.gene053311.t1 MTR_8g077460 41.205 415 195 15 13 419 2 375 3.71e-74 236
MS.gene053311.t1 MTR_8g468760 37.832 452 196 13 16 418 3 418 4.02e-69 225
MS.gene053311.t1 MTR_8g468810 41.979 374 161 11 6 329 4 371 6.61e-69 223
MS.gene053311.t1 MTR_8g445780 42.239 393 174 13 20 372 11 390 2.59e-68 222
MS.gene053311.t1 MTR_8g468810 43.189 301 123 8 6 263 4 299 6.05e-60 198
MS.gene053311.t1 MTR_8g468810 43.189 301 123 8 6 263 4 299 1.03e-59 197
MS.gene053311.t1 MTR_2g086020 69.178 146 45 0 279 424 175 320 1.30e-59 197
MS.gene053311.t1 MTR_2g086020 50.658 152 59 4 48 193 1 142 5.36e-34 129
MS.gene053311.t1 MTR_8g468810 43.189 301 123 8 6 263 4 299 1.51e-59 198
MS.gene053311.t1 MTR_8g014160 36.156 437 188 18 22 419 41 425 6.34e-57 193
MS.gene053311.t1 MTR_5g092960 36.364 363 174 9 18 329 15 371 2.40e-50 175
MS.gene053311.t1 MTR_4g024710 41.494 241 123 8 181 419 123 347 6.26e-42 151
MS.gene053311.t1 MTR_8g014210 43.750 224 117 7 201 419 12 231 1.32e-40 144
MS.gene053311.t1 MTR_8g467800 40.317 315 139 11 6 279 3 309 3.69e-40 147
MS.gene053311.t1 MTR_1g041245 32.530 415 226 19 21 419 10 386 5.48e-38 142
MS.gene053311.t1 MTR_5g021550 50.610 164 72 7 5 163 3 162 6.92e-36 130
MS.gene053311.t1 MTR_5g008950 29.238 407 231 14 26 419 3 365 7.61e-33 127
MS.gene053311.t1 MTR_7g115270 27.670 412 257 15 22 416 12 399 1.22e-31 124
MS.gene053311.t1 MTR_8g014290 42.500 160 82 4 13 163 1 159 3.77e-30 114
MS.gene053311.t1 MTR_4g024745 39.732 224 117 8 181 402 77 284 6.96e-30 117
MS.gene053311.t1 MTR_7g056433 29.369 412 253 13 17 419 7 389 7.10e-29 117
MS.gene053311.t1 MTR_4g024720 43.820 178 74 7 6 163 5 176 2.87e-28 110
MS.gene053311.t1 MTR_7g098510 29.698 431 247 18 5 419 1 391 7.86e-28 114
MS.gene053311.t1 MTR_5g084070 26.591 440 266 13 7 411 2 419 8.53e-28 114
MS.gene053311.t1 MTR_7g026500 28.467 411 254 13 22 420 14 396 1.24e-25 108
MS.gene053311.t1 MTR_3g108150 28.851 409 248 13 20 418 11 386 2.39e-25 107
MS.gene053311.t1 MTR_3g107100 28.401 419 260 17 18 421 7 400 3.68e-25 106
MS.gene053311.t1 MTR_7g034115 30.900 411 219 19 21 411 10 375 7.04e-25 105
MS.gene053311.t1 MTR_6g045333 27.442 430 264 14 3 418 50 445 7.45e-25 106
MS.gene053311.t1 MTR_7g033915 28.362 409 230 18 20 418 9 364 1.50e-24 104
MS.gene053311.t1 MTR_5g080710 26.457 446 272 14 6 411 17 446 4.30e-24 104
MS.gene053311.t1 MTR_3g105060 30.073 409 226 18 22 419 14 373 4.30e-24 103
MS.gene053311.t1 MTR_3g071850 29.280 403 247 11 22 411 24 401 7.53e-24 104
MS.gene053311.t1 MTR_7g032370 28.141 398 227 17 34 419 2 352 9.05e-24 102
MS.gene053311.t1 MTR_3g099800 56.322 87 37 1 331 416 19 105 1.54e-23 94.7
MS.gene053311.t1 MTR_1089s0010 25.604 414 262 15 16 419 2 379 4.27e-23 100
MS.gene053311.t1 MTR_2g036470 27.751 418 267 16 12 418 2 395 1.57e-22 99.0
MS.gene053311.t1 MTR_7g033990 28.087 413 230 15 22 413 11 377 2.21e-22 99.0
MS.gene053311.t1 MTR_7g098480 27.209 430 255 16 5 418 1 388 6.09e-22 98.2
MS.gene053311.t1 MTR_0543s0010 27.561 410 238 14 22 418 86 449 1.52e-21 96.7
MS.gene053311.t1 MTR_5g008920 27.407 405 242 15 27 419 17 381 5.85e-21 94.4
MS.gene053311.t1 MTR_6g011460 26.995 426 265 14 6 419 30 421 7.10e-20 91.7
MS.gene053311.t1 MTR_5g073420 28.052 385 216 17 22 391 11 349 1.07e-19 90.5
MS.gene053311.t1 MTR_3g036250 26.829 410 240 14 19 416 2 363 1.21e-19 90.1
MS.gene053311.t1 MTR_4g098590 28.792 389 214 18 25 386 2 354 2.13e-19 89.7
MS.gene053311.t1 MTR_3g108560 27.094 406 256 13 19 418 17 388 2.60e-19 89.7
MS.gene053311.t1 MTR_1g108820 28.606 409 247 19 21 416 7 383 1.18e-18 87.4
MS.gene053311.t1 MTR_7g056420 27.182 401 233 14 22 413 9 359 1.24e-18 89.0
MS.gene053311.t1 MTR_7g056430 27.182 401 233 14 22 413 9 359 1.34e-18 89.0
MS.gene053311.t1 MTR_7g056447 32.768 177 106 6 17 193 4 167 1.78e-18 85.9
MS.gene053311.t1 MTR_5g045170 27.136 398 258 13 25 416 17 388 2.98e-18 86.7
MS.gene053311.t1 MTR_6g040270 27.182 401 250 17 25 416 11 378 1.11e-17 84.7
MS.gene053311.t1 MTR_2g008310 26.329 414 245 16 22 419 2 371 2.95e-17 83.6
MS.gene053311.t1 MTR_7g055943 24.828 435 275 17 3 419 21 421 6.17e-17 82.8
MS.gene053311.t1 MTR_6g022500 27.317 410 252 15 22 419 101 476 6.55e-17 82.8
MS.gene053311.t1 MTR_0009s0080 25.515 388 234 10 27 394 23 375 1.42e-16 81.6
MS.gene053311.t1 MTR_0492s0020 26.984 378 217 14 22 386 86 417 1.84e-16 81.3
MS.gene053311.t1 MTR_7g032380 38.012 171 87 9 22 188 11 166 2.61e-16 80.1
MS.gene053311.t1 MTR_7g032380 34.074 135 80 4 292 419 224 356 1.04e-11 66.2
MS.gene053311.t1 MTR_8g014230 45.238 84 44 2 329 412 17 98 2.89e-16 74.7
MS.gene053311.t1 MTR_8g085430 34.444 180 102 7 21 194 10 179 3.34e-16 79.7
MS.gene053311.t1 MTR_5g045510 25.301 415 243 15 22 421 12 374 6.79e-16 79.3
MS.gene053311.t1 MTR_5g058000 28.087 413 251 18 13 418 1 374 9.77e-16 78.6
MS.gene053311.t1 MTR_3g087260 26.793 474 241 20 22 418 12 456 1.08e-15 79.3
MS.gene053311.t1 MTR_7g034110 27.809 356 182 13 20 369 9 295 1.17e-15 78.2
MS.gene053311.t1 MTR_5g073370 27.540 374 221 16 19 378 2 339 2.02e-15 77.8
MS.gene053311.t1 MTR_7g033945 33.146 178 101 8 20 191 9 174 3.84e-15 73.6
MS.gene053311.t1 MTR_3g082890 35.152 165 94 6 7 171 2 153 1.30e-14 75.9
MS.gene053311.t1 MTR_3g082890 34.031 191 111 8 1 189 76 253 1.42e-14 75.9
MS.gene053311.t1 MTR_2g007500 25.275 364 213 15 21 366 13 335 3.96e-14 73.6
MS.gene053311.t1 MTR_2g007450 31.250 192 120 4 8 188 2 192 3.96e-14 74.3
MS.gene053311.t1 MTR_4g024770 52.239 67 26 2 1 67 8 68 7.39e-14 67.0
MS.gene053311.t1 MTR_5g083890 26.884 398 231 18 22 387 6 375 8.23e-14 73.6
MS.gene053311.t1 MTR_1g090900 35.429 175 100 7 15 188 4 166 1.59e-13 69.7
MS.gene053311.t1 MTR_2g005990 25.701 428 248 17 21 417 5 393 3.85e-13 70.9
MS.gene053311.t1 MTR_5g058100 34.104 173 100 6 16 186 3 163 7.27e-13 70.1
MS.gene053311.t1 MTR_2g008320 34.254 181 100 7 14 188 44 211 1.03e-12 70.1
MS.gene053311.t1 MTR_4g093690 33.149 181 91 10 18 189 14 173 1.06e-12 68.6
MS.gene053311.t1 MTR_6g022390 34.400 125 74 4 22 140 5 127 1.10e-12 65.9
MS.gene053311.t1 MTR_2g082800 30.435 161 95 6 7 166 17 161 1.40e-12 69.3
MS.gene053311.t1 MTR_3g115430 36.364 110 66 2 310 419 20 125 2.08e-12 64.3
MS.gene053311.t1 MTR_1g038710 28.755 233 144 8 20 247 17 232 2.28e-12 68.9
MS.gene053311.t1 MTR_4g056020 35.429 175 91 7 17 186 4 161 5.28e-12 67.8
MS.gene053311.t1 MTR_8g066650 33.696 184 95 7 8 186 67 228 7.73e-12 67.4
MS.gene053311.t1 MTR_8g014180 55.932 59 21 2 6 64 5 58 9.99e-12 60.5
MS.gene053311.t1 MTR_2g082880 23.953 430 242 17 13 386 1 401 1.17e-11 66.6
MS.gene053311.t1 MTR_5g013500 25.120 418 232 18 22 419 9 365 1.25e-11 66.2
MS.gene053311.t1 MTR_2g020170 33.058 121 75 3 20 140 20 134 1.33e-11 62.4
MS.gene053311.t1 MTR_2g072530 31.293 147 91 4 20 165 11 148 2.84e-11 65.5
MS.gene053311.t1 MTR_3g110010 30.534 262 143 12 22 263 13 255 3.09e-11 65.9
MS.gene053311.t1 MTR_3g467400 32.597 181 97 8 21 186 2 172 3.27e-11 65.1
MS.gene053311.t1 MTR_3g109982 30.534 262 143 12 22 263 13 255 3.40e-11 65.9
MS.gene053311.t1 MTR_5g012840 38.182 110 65 3 310 419 212 318 3.62e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053311.t1 AT5G56420 26.471 442 256 15 22 419 6 422 1.05e-21 97.1
MS.gene053311.t1 AT5G56420 26.471 442 256 15 22 419 6 422 1.05e-21 97.1
MS.gene053311.t1 AT5G56420 26.471 442 256 15 22 419 6 422 1.05e-21 97.1
MS.gene053311.t1 AT2G26030 24.034 466 256 17 20 416 2 438 5.53e-20 92.0
MS.gene053311.t1 AT2G26030 24.034 466 256 17 20 416 2 438 5.53e-20 92.0
MS.gene053311.t1 AT2G26030 23.605 466 263 17 20 416 2 443 1.58e-19 90.9
MS.gene053311.t1 AT4G10400 35.329 167 98 5 22 188 2 158 6.87e-18 85.5
MS.gene053311.t1 AT4G10400 35.329 167 98 5 22 188 2 158 6.87e-18 85.5
MS.gene053311.t1 AT4G10400 35.329 167 98 5 22 188 2 158 6.87e-18 85.5
MS.gene053311.t1 AT4G09920 34.731 167 99 5 22 188 2 158 1.40e-17 83.2
MS.gene053311.t1 AT4G09920 34.731 167 99 5 22 188 2 158 1.40e-17 83.2
MS.gene053311.t1 AT4G09920 34.731 167 99 5 22 188 2 158 1.40e-17 83.2
MS.gene053311.t1 AT4G09920 34.731 167 99 5 22 188 2 158 1.76e-17 83.2
MS.gene053311.t1 AT4G09920 34.731 167 99 5 22 188 2 158 1.76e-17 83.2
MS.gene053311.t1 AT4G26340 25.339 442 262 15 22 419 2 419 3.39e-17 83.6
MS.gene053311.t1 AT1G69630 32.778 180 111 5 9 188 5 174 3.59e-17 83.6
MS.gene053311.t1 AT3G50710 26.949 449 254 18 22 419 2 427 8.00e-17 82.4
MS.gene053311.t1 AT3G59000 31.884 207 128 5 22 222 2 201 9.90e-17 82.4
MS.gene053311.t1 AT5G22610 23.898 431 279 14 22 417 18 434 1.07e-16 82.4
MS.gene053311.t1 AT3G59200 32.948 173 101 5 22 189 7 169 7.33e-16 79.0
MS.gene053311.t1 AT3G59200 32.948 173 101 5 22 189 7 169 1.35e-15 79.0
MS.gene053311.t1 AT5G38570 25.854 410 255 14 22 418 2 375 2.72e-15 77.4
MS.gene053311.t1 AT3G03040 34.211 152 87 4 22 168 2 145 7.31e-15 76.6
MS.gene053311.t1 AT2G42720 32.778 180 108 6 22 197 2 172 8.49e-15 76.3
MS.gene053311.t1 AT3G59230 30.660 212 123 7 22 220 12 212 1.02e-14 76.3
MS.gene053311.t1 AT1G06630 31.176 170 110 4 22 188 12 177 1.16e-14 74.7
MS.gene053311.t1 AT1G06630 31.176 170 110 4 22 188 12 177 1.21e-14 75.5
MS.gene053311.t1 AT1G06630 31.176 170 110 4 22 188 12 177 1.21e-14 75.5
MS.gene053311.t1 AT1G06630 31.176 170 110 4 22 188 12 177 1.64e-14 75.5
MS.gene053311.t1 AT1G06630 31.176 170 110 4 22 188 12 177 1.64e-14 75.5
MS.gene053311.t1 AT1G06630 31.176 170 110 4 22 188 12 177 1.64e-14 75.5
MS.gene053311.t1 AT1G06630 31.176 170 110 4 22 188 12 177 1.64e-14 75.5
MS.gene053311.t1 AT3G59190 31.016 187 110 6 5 186 1 173 2.18e-14 74.7
MS.gene053311.t1 AT1G06630 31.176 170 110 4 22 188 21 186 2.18e-14 75.1
MS.gene053311.t1 AT1G06630 31.176 170 110 4 22 188 12 177 2.78e-14 75.1
MS.gene053311.t1 AT3G59190 31.765 170 103 5 22 186 12 173 2.82e-14 75.1
MS.gene053311.t1 AT1G16930 30.726 179 110 5 20 197 13 178 3.06e-14 74.7
MS.gene053311.t1 AT1G16930 30.726 179 110 5 20 197 13 178 3.06e-14 74.7
MS.gene053311.t1 AT1G16930 30.726 179 110 5 20 197 13 178 3.06e-14 74.7
MS.gene053311.t1 AT3G58920 23.918 485 264 17 22 419 2 468 1.32e-13 72.8
MS.gene053311.t1 AT5G50270 25.362 414 239 12 22 386 2 394 1.59e-13 72.4
MS.gene053311.t1 AT3G60040 31.183 186 111 5 16 189 7 187 1.62e-13 72.8
MS.gene053311.t1 AT3G53550 26.199 271 153 10 150 420 19 242 2.59e-13 70.1
MS.gene053311.t1 AT3G60040 31.183 186 111 5 16 189 226 406 3.04e-13 72.4
MS.gene053311.t1 AT3G60040 31.183 186 111 5 16 189 227 407 3.09e-13 72.4
MS.gene053311.t1 AT5G38392 34.667 150 85 5 22 165 2 144 3.18e-13 68.9
MS.gene053311.t1 AT3G60040 31.183 186 111 5 16 189 224 404 3.55e-13 72.0
MS.gene053311.t1 AT3G60040 31.183 186 111 5 16 189 226 406 3.78e-13 71.6
MS.gene053311.t1 AT3G59000 37.398 123 67 3 22 139 2 119 4.00e-13 70.5
MS.gene053311.t1 AT5G41630 32.571 175 102 6 22 186 12 180 4.86e-13 70.9
MS.gene053311.t1 AT5G44850 25.667 300 199 9 120 416 50 328 5.27e-13 70.1
MS.gene053311.t1 AT4G14103 30.556 180 106 5 16 189 2 168 7.50e-13 70.1
MS.gene053311.t1 AT4G14103 30.556 180 106 5 16 189 2 168 7.50e-13 70.1
MS.gene053311.t1 AT3G58930 29.670 182 113 5 22 193 2 178 8.57e-13 70.5
MS.gene053311.t1 AT3G58930 29.670 182 113 5 22 193 2 178 8.57e-13 70.5
MS.gene053311.t1 AT3G58930 29.670 182 113 5 22 193 2 178 8.57e-13 70.5
MS.gene053311.t1 AT4G14103 30.556 180 106 5 16 189 2 168 9.44e-13 70.1
MS.gene053311.t1 AT3G44060 31.902 163 105 3 27 189 4 160 1.24e-12 69.7
MS.gene053311.t1 AT5G56410 32.370 173 95 6 29 186 10 175 4.03e-12 68.2
MS.gene053311.t1 AT3G59240 29.480 173 105 5 22 188 8 169 8.52e-12 67.4
MS.gene053311.t1 AT5G53840 31.214 173 98 10 20 189 16 170 1.46e-11 66.2
MS.gene053311.t1 AT4G15060 30.588 170 107 6 19 186 23 183 1.70e-11 66.2
MS.gene053311.t1 AT1G05080 32.164 171 98 6 22 188 13 169 2.19e-11 65.9
MS.gene053311.t1 AT5G22720 23.454 469 260 16 22 418 23 464 2.56e-11 65.9
MS.gene053311.t1 AT1G66310 29.545 176 105 7 17 189 14 173 3.97e-11 65.1
MS.gene053311.t1 AT5G50270 32.738 168 102 5 22 188 2 159 9.40e-11 63.2

Find 103 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGATCCCATGTTCTTAA+AGG 0.222652 8.3:+39406397 None:intergenic
CTCGCCGTTGGCTCCACCTT+TGG 0.226704 8.3:-39407814 MS.gene053311:CDS
TTGGGAGGAAGGAGAGTATA+TGG 0.278362 8.3:+39407870 None:intergenic
TTCGGAACTGTTGTTGGCTT+TGG 0.282349 8.3:+39406379 None:intergenic
ATGATGTAAATTGCAAAATT+CGG 0.282467 8.3:+39406650 None:intergenic
CTCACTATTGACTGTGGCTT+TGG 0.287049 8.3:-39407560 MS.gene053311:CDS
TCGCCGTTGGCTCCACCTTT+GGG 0.301924 8.3:-39407813 MS.gene053311:CDS
ATTCGGGATGGTTTAGAATA+GGG 0.329472 8.3:+39406667 None:intergenic
GAGAACGATACTTGTTAGTT+AGG 0.329534 8.3:+39406218 None:intergenic
CATAGTCGCAACGGAGGTTC+TGG 0.354729 8.3:+39407847 None:intergenic
TGATGTAAATTGCAAAATTC+GGG 0.366477 8.3:+39406651 None:intergenic
GACTCAACCGTGTCTCCTTA+AGG 0.366570 8.3:-39406142 None:intergenic
CCACCTTTGGGAGCATCTAC+AGG 0.370248 8.3:-39407801 MS.gene053311:CDS
AACACACACTGCAGTCTTGC+TGG 0.372624 8.3:-39407222 MS.gene053311:intron
GACAGATCCCCTTGGTATAT+CGG 0.376237 8.3:+39406183 None:intergenic
GTCTTCAAATAGATGCTGAT+TGG 0.384029 8.3:-39406968 MS.gene053311:CDS
CCGGCGAATGCGTCGATATG+TGG 0.392734 8.3:-39407622 MS.gene053311:CDS
AATTCGGGATGGTTTAGAAT+AGG 0.410222 8.3:+39406666 None:intergenic
AGGTGGGATTCAAGACATTT+CGG 0.410378 8.3:+39406361 None:intergenic
TAACCGCTACAAATTTCTCT+TGG 0.416055 8.3:-39406995 MS.gene053311:CDS
ATGTGGGTCCGTGCTGCCAT+TGG 0.431274 8.3:-39407605 MS.gene053311:CDS
AGACATTTCGGAACTGTTGT+TGG 0.431407 8.3:+39406373 None:intergenic
CCGTCGATGTCCACAATGCC+AGG 0.431982 8.3:+39407132 None:intergenic
CAAAAGATTCATGTTGAAGC+AGG 0.443364 8.3:+39406611 None:intergenic
CTGACCTGTATTCACATAGA+AGG 0.453510 8.3:-39406340 MS.gene053311:CDS
AGGAGGGAGGAGAACTAGTT+GGG 0.454955 8.3:+39407535 None:intergenic
AAAACTACGTTAGGTATCAT+TGG 0.457269 8.3:-39406927 MS.gene053311:CDS
TTCGGGATGGTTTAGAATAG+GGG 0.458860 8.3:+39406668 None:intergenic
ATATCCTTCTATGTGAATAC+AGG 0.460963 8.3:+39406336 None:intergenic
AAGGAGGGAGGAGAACTAGT+TGG 0.461653 8.3:+39407534 None:intergenic
TGTGGGTCCGTGCTGCCATT+GGG 0.463012 8.3:-39407604 MS.gene053311:CDS
CCACATATCGACGCATTCGC+CGG 0.463774 8.3:+39407622 None:intergenic
TGCAGTTGAAGAAGGAAGGA+GGG 0.473975 8.3:+39407519 None:intergenic
TTGGTGCAGTTGAAGAAGGA+AGG 0.476644 8.3:+39407515 None:intergenic
CGACGACATGAGCGGTAACC+AGG 0.478779 8.3:-39407765 MS.gene053311:CDS
CTACATGAGGGAAACGAGAT+TGG 0.483024 8.3:+39407496 None:intergenic
AAGTACGGTAAAACTACGTT+AGG 0.485264 8.3:-39406936 MS.gene053311:CDS
ATATGGGAAAGAAGAGAGTC+CGG 0.487274 8.3:+39407887 None:intergenic
TGATGAGAACTTGAAGCATA+TGG 0.490680 8.3:+39406568 None:intergenic
GGAGGTTCTGGTTGGGAGGA+AGG 0.491388 8.3:+39407859 None:intergenic
CATGGAGGTGTTTGAGCATA+GGG 0.492369 8.3:+39406838 None:intergenic
TTCTTGAAGGTGGGGCCCAA+TGG 0.492905 8.3:+39407589 None:intergenic
GTGCAGTTGAAGAAGGAAGG+AGG 0.497666 8.3:+39407518 None:intergenic
TTCGAATGTCGCGGGATCTA+CGG 0.498527 8.3:+39407687 None:intergenic
TCCCATGTTCTTAAAGGTGA+TGG 0.501184 8.3:+39406403 None:intergenic
TGGGAGGAAGGAGAGTATAT+GGG 0.514178 8.3:+39407871 None:intergenic
CTTCTATGTGAATACAGGTC+AGG 0.515434 8.3:+39406341 None:intergenic
CACATATCGACGCATTCGCC+GGG 0.528256 8.3:+39407623 None:intergenic
TCATGGAGGTGTTTGAGCAT+AGG 0.529363 8.3:+39406837 None:intergenic
TAGTGAGATCAAGTTCTTGA+AGG 0.531895 8.3:+39407576 None:intergenic
TCAAGGATTTGAAGATGAGC+TGG 0.532840 8.3:-39406314 MS.gene053311:CDS
CGGTAGAGTACTAAATCTAT+CGG 0.540812 8.3:+39407907 None:intergenic
TCGCAACGGAGGTTCTGGTT+GGG 0.542010 8.3:+39407852 None:intergenic
ATTCACATAGAAGGATATCA+AGG 0.543245 8.3:-39406331 MS.gene053311:CDS
GAGATCAAGTTCTTGAAGGT+GGG 0.544436 8.3:+39407580 None:intergenic
AACCATCACCTTTAAGAACA+TGG 0.545612 8.3:-39406405 MS.gene053311:CDS
CGGCGAATGCGTCGATATGT+GGG 0.546745 8.3:-39407621 MS.gene053311:CDS
GTCGCAACGGAGGTTCTGGT+TGG 0.555045 8.3:+39407851 None:intergenic
GCTCCCAAAGGTGGAGCCAA+CGG 0.556367 8.3:+39407810 None:intergenic
CGATAGATTTAGTACTCTAC+CGG 0.556997 8.3:-39407906 MS.gene053311:CDS
GAGATTGGTGCAGTTGAAGA+AGG 0.557300 8.3:+39407511 None:intergenic
TGACTCTCGTCTCTCGCCGT+TGG 0.562504 8.3:-39407826 MS.gene053311:CDS
AGTTGAAGAAGGAAGGAGGG+AGG 0.564433 8.3:+39407522 None:intergenic
GACGAGAGTCATAGTCGCAA+CGG 0.565226 8.3:+39407838 None:intergenic
GTAAATTGCAAAATTCGGGA+TGG 0.567843 8.3:+39406655 None:intergenic
TGAGATCAAGTTCTTGAAGG+TGG 0.572287 8.3:+39407579 None:intergenic
AGATCAAGTTCTTGAAGGTG+GGG 0.573241 8.3:+39407581 None:intergenic
CCTGTAGATGCTCCCAAAGG+TGG 0.579626 8.3:+39407801 None:intergenic
GACAAATTTACTTTCACGCA+GGG 0.581653 8.3:+39406863 None:intergenic
AATGTCGCGGGATCTACGGC+GGG 0.584290 8.3:+39407691 None:intergenic
ACGGAACTTTCGAATGTCGC+GGG 0.584834 8.3:+39407679 None:intergenic
CGGAGATACTGCATCTGAGA+CGG 0.585392 8.3:+39407660 None:intergenic
ACCATCACCTTTAAGAACAT+GGG 0.588734 8.3:-39406404 MS.gene053311:CDS
CTATGTGAATACAGGTCAGG+TGG 0.590917 8.3:+39406344 None:intergenic
CCTGGCATTGTGGACATCGA+CGG 0.594159 8.3:-39407132 MS.gene053311:CDS
TTTGCGTCATTCGAAAACAA+AGG 0.600766 8.3:-39406790 MS.gene053311:intron
AAAGATTAACCGATATACCA+AGG 0.607874 8.3:-39406192 MS.gene053311:CDS
AAGATTAACCGATATACCAA+GGG 0.609206 8.3:-39406191 MS.gene053311:CDS
GACGGAACTTTCGAATGTCG+CGG 0.610614 8.3:+39407678 None:intergenic
AAAAGATTCATGTTGAAGCA+GGG 0.613622 8.3:+39406612 None:intergenic
CAACGGAGGTTCTGGTTGGG+AGG 0.615004 8.3:+39407855 None:intergenic
GAACAGAACGACGATGGCAA+CGG 0.622946 8.3:-39407981 None:intergenic
GGTGGGGCCCAATGGCAGCA+CGG 0.623491 8.3:+39407597 None:intergenic
CATTGGCAACTTGCAGACTG+TGG 0.632179 8.3:-39406910 MS.gene053311:CDS
GACTTCGACGACGACATGAG+CGG 0.637063 8.3:-39407773 MS.gene053311:CDS
TATGTGAATACAGGTCAGGT+GGG 0.643030 8.3:+39406345 None:intergenic
CGACAAATTTACTTTCACGC+AGG 0.643788 8.3:+39406862 None:intergenic
GAATGTCGCGGGATCTACGG+CGG 0.644193 8.3:+39407690 None:intergenic
TGGCAACTTGCAGACTGTGG+AGG 0.644944 8.3:-39406907 MS.gene053311:CDS
TCTCATCATTCAGAGCAGCA+AGG 0.648834 8.3:-39406553 MS.gene053311:intron
CGTCGATGTCCACAATGCCA+GGG 0.648850 8.3:+39407133 None:intergenic
AAGAATGTTGAATCTTGACA+AGG 0.659316 8.3:+39407193 None:intergenic
CTTGATCTCACTATTGACTG+TGG 0.661111 8.3:-39407566 MS.gene053311:CDS
GATTGGCTTGATGTAAAGTA+CGG 0.661486 8.3:-39406951 MS.gene053311:CDS
GAAAATCGACCCTGGCATTG+TGG 0.661523 8.3:-39407142 MS.gene053311:CDS
GAGAGTCATAGTCGCAACGG+AGG 0.665906 8.3:+39407841 None:intergenic
AGTCCAAGAGAAATTTGTAG+CGG 0.669486 8.3:+39406992 None:intergenic
TTTAGAATAGGGGTGTGAAG+TGG 0.674978 8.3:+39406678 None:intergenic
AAACCTGTAGATGCTCCCAA+AGG 0.675107 8.3:+39407798 None:intergenic
TGACACGTGACAGATCCCCT+TGG 0.678952 8.3:+39406175 None:intergenic
GCTTGCACCTTAAGGAGACA+CGG 0.689601 8.3:+39406135 None:intergenic
AAGATGTTGAAAATCGACCC+TGG 0.690890 8.3:-39407150 MS.gene053311:CDS
AGATTAACCGATATACCAAG+GGG 0.738387 8.3:-39406190 MS.gene053311:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAAAAAGTTGTATATCA+CGG + chr8.3:39406826-39406845 None:intergenic 15.0%
!!! AGGTTAATTAAATCAATTTT+TGG - chr8.3:39407597-39407616 MS.gene053311:CDS 15.0%
!! ATGATGTAAATTGCAAAATT+CGG + chr8.3:39407465-39407484 None:intergenic 20.0%
! TGATGTAAATTGCAAAATTC+GGG + chr8.3:39407464-39407483 None:intergenic 25.0%
! TTCCAAATTATTAGTTGTGA+TGG - chr8.3:39406770-39406789 MS.gene053311:intron 25.0%
!! AATGAAGATCAATTTTGTGA+TGG + chr8.3:39406666-39406685 None:intergenic 25.0%
!! AGTATCAAAAGTTTCAAGTA+TGG + chr8.3:39407050-39407069 None:intergenic 25.0%
!! GTATCAAAAGTTTCAAGTAT+GGG + chr8.3:39407049-39407068 None:intergenic 25.0%
!!! ATTATTTGTTATCTGTACAG+TGG - chr8.3:39407411-39407430 MS.gene053311:intron 25.0%
!!! GTAAATCATGTTTTTGATCA+TGG + chr8.3:39407295-39407314 None:intergenic 25.0%
AAAACTACGTTAGGTATCAT+TGG - chr8.3:39407185-39407204 MS.gene053311:CDS 30.0%
AAAAGATTCATGTTGAAGCA+GGG + chr8.3:39407503-39407522 None:intergenic 30.0%
AAAGATTAACCGATATACCA+AGG - chr8.3:39407920-39407939 MS.gene053311:CDS 30.0%
AAAGTTGTATATCACGGATA+CGG + chr8.3:39406820-39406839 None:intergenic 30.0%
AAGAATGTTGAATCTTGACA+AGG + chr8.3:39406922-39406941 None:intergenic 30.0%
AAGATTAACCGATATACCAA+GGG - chr8.3:39407921-39407940 MS.gene053311:CDS 30.0%
ATATCCTTCTATGTGAATAC+AGG + chr8.3:39407779-39407798 None:intergenic 30.0%
ATTCACATAGAAGGATATCA+AGG - chr8.3:39407781-39407800 MS.gene053311:CDS 30.0%
CTCCATCACAACTAATAATT+TGG + chr8.3:39406775-39406794 None:intergenic 30.0%
! AACTGCACAAAATTTTCAGA+AGG - chr8.3:39406370-39406389 MS.gene053311:CDS 30.0%
! ACAGAATATATTTTGCGTCA+CGG - chr8.3:39407826-39407845 MS.gene053311:CDS 30.0%
! ATTTTCAACATCTTGAGTGA+CGG + chr8.3:39406956-39406975 None:intergenic 30.0%
! TACATAATTAAGTGTCGTGT+CGG + chr8.3:39406743-39406762 None:intergenic 30.0%
!! AATCATGTTTTTGATCATGG+AGG + chr8.3:39407292-39407311 None:intergenic 30.0%
!! AATTTTAATCTCTTGGAGGA+AGG + chr8.3:39407098-39407117 None:intergenic 30.0%
!! AGCGGTTAATTTTAATCTCT+TGG + chr8.3:39407105-39407124 None:intergenic 30.0%
!! GGTTAATTTTAATCTCTTGG+AGG + chr8.3:39407102-39407121 None:intergenic 30.0%
AAGTACGGTAAAACTACGTT+AGG - chr8.3:39407176-39407195 MS.gene053311:CDS 35.0%
AGATTAACCGATATACCAAG+GGG - chr8.3:39407922-39407941 MS.gene053311:CDS 35.0%
AGTCCAAGAGAAATTTGTAG+CGG + chr8.3:39407123-39407142 None:intergenic 35.0%
CAAAAGATTCATGTTGAAGC+AGG + chr8.3:39407504-39407523 None:intergenic 35.0%
CGATAGATTTAGTACTCTAC+CGG - chr8.3:39406206-39406225 MS.gene053311:CDS 35.0%
GAAATAACCTACCTACATGA+GGG + chr8.3:39406631-39406650 None:intergenic 35.0%
GACAAATTTACTTTCACGCA+GGG + chr8.3:39407252-39407271 None:intergenic 35.0%
GTAAATTGCAAAATTCGGGA+TGG + chr8.3:39407460-39407479 None:intergenic 35.0%
GTCTTCAAATAGATGCTGAT+TGG - chr8.3:39407144-39407163 MS.gene053311:CDS 35.0%
TAACCGCTACAAATTTCTCT+TGG - chr8.3:39407117-39407136 MS.gene053311:intron 35.0%
TAGTGAGATCAAGTTCTTGA+AGG + chr8.3:39406539-39406558 None:intergenic 35.0%
TGAAATAACCTACCTACATG+AGG + chr8.3:39406632-39406651 None:intergenic 35.0%
TGATGAGAACTTGAAGCATA+TGG + chr8.3:39407547-39407566 None:intergenic 35.0%
TTTGCGTCATTCGAAAACAA+AGG - chr8.3:39407322-39407341 MS.gene053311:intron 35.0%
TTTGGATCCCATGTTCTTAA+AGG + chr8.3:39407718-39407737 None:intergenic 35.0%
! AACCATCACCTTTAAGAACA+TGG - chr8.3:39407707-39407726 MS.gene053311:CDS 35.0%
! AATTCGGGATGGTTTAGAAT+AGG + chr8.3:39407449-39407468 None:intergenic 35.0%
! ACCATCACCTTTAAGAACAT+GGG - chr8.3:39407708-39407727 MS.gene053311:CDS 35.0%
! ATTCGGGATGGTTTAGAATA+GGG + chr8.3:39407448-39407467 None:intergenic 35.0%
! CAGAATATATTTTGCGTCAC+GGG - chr8.3:39407827-39407846 MS.gene053311:CDS 35.0%
! CGGTAGAGTACTAAATCTAT+CGG + chr8.3:39406208-39406227 None:intergenic 35.0%
! GAAGATCAATTTTGTGATGG+TGG + chr8.3:39406663-39406682 None:intergenic 35.0%
! GAGAACGATACTTGTTAGTT+AGG + chr8.3:39407897-39407916 None:intergenic 35.0%
! TCCGATTTTTACAACTTTCC+CGG - chr8.3:39406471-39406490 MS.gene053311:intron 35.0%
! TGAAAATTTTGTGCAGTTCC+TGG + chr8.3:39406368-39406387 None:intergenic 35.0%
!! CAAGGTTTGTTAAGTTCTCA+AGG - chr8.3:39407577-39407596 MS.gene053311:CDS 35.0%
!! GATTGGCTTGATGTAAAGTA+CGG - chr8.3:39407161-39407180 MS.gene053311:CDS 35.0%
AAGATGTTGAAAATCGACCC+TGG - chr8.3:39406962-39406981 MS.gene053311:CDS 40.0%
AGACATTTCGGAACTGTTGT+TGG + chr8.3:39407742-39407761 None:intergenic 40.0%
AGATCAAGTTCTTGAAGGTG+GGG + chr8.3:39406534-39406553 None:intergenic 40.0%
AGGTGGGATTCAAGACATTT+CGG + chr8.3:39407754-39407773 None:intergenic 40.0%
ATATGGGAAAGAAGAGAGTC+CGG + chr8.3:39406228-39406247 None:intergenic 40.0%
CCAGAGAGAAAGTCAATTAG+TGG + chr8.3:39407022-39407041 None:intergenic 40.0%
CGACAAATTTACTTTCACGC+AGG + chr8.3:39407253-39407272 None:intergenic 40.0%
CTGACCTGTATTCACATAGA+AGG - chr8.3:39407772-39407791 MS.gene053311:CDS 40.0%
CTTCTATGTGAATACAGGTC+AGG + chr8.3:39407774-39407793 None:intergenic 40.0%
GAGATCAAGTTCTTGAAGGT+GGG + chr8.3:39406535-39406554 None:intergenic 40.0%
GCCGGGAAAGTTGTAAAAAT+CGG + chr8.3:39406475-39406494 None:intergenic 40.0%
TATGTGAATACAGGTCAGGT+GGG + chr8.3:39407770-39407789 None:intergenic 40.0%
TCAAGGATTTGAAGATGAGC+TGG - chr8.3:39407798-39407817 MS.gene053311:CDS 40.0%
TCCCATGTTCTTAAAGGTGA+TGG + chr8.3:39407712-39407731 None:intergenic 40.0%
TGAGATCAAGTTCTTGAAGG+TGG + chr8.3:39406536-39406555 None:intergenic 40.0%
TTTAATCTCTTGGAGGAAGG+TGG + chr8.3:39407095-39407114 None:intergenic 40.0%
! CCACTAATTGACTTTCTCTC+TGG - chr8.3:39407019-39407038 MS.gene053311:intron 40.0%
! CTTGATCTCACTATTGACTG+TGG - chr8.3:39406546-39406565 MS.gene053311:intron 40.0%
! TTCGGGATGGTTTAGAATAG+GGG + chr8.3:39407447-39407466 None:intergenic 40.0%
! TTTAGAATAGGGGTGTGAAG+TGG + chr8.3:39407437-39407456 None:intergenic 40.0%
AAACCTGTAGATGCTCCCAA+AGG + chr8.3:39406317-39406336 None:intergenic 45.0%
CAATCTCGTTTCCCTCATGT+AGG - chr8.3:39406617-39406636 MS.gene053311:CDS 45.0%
CTACATGAGGGAAACGAGAT+TGG + chr8.3:39406619-39406638 None:intergenic 45.0%
CTATGTGAATACAGGTCAGG+TGG + chr8.3:39407771-39407790 None:intergenic 45.0%
GACAGATCCCCTTGGTATAT+CGG + chr8.3:39407932-39407951 None:intergenic 45.0%
GAGATTGGTGCAGTTGAAGA+AGG + chr8.3:39406604-39406623 None:intergenic 45.0%
GTATATCACGGATACGGACA+TGG + chr8.3:39406814-39406833 None:intergenic 45.0%
TCTCATCATTCAGAGCAGCA+AGG - chr8.3:39407559-39407578 MS.gene053311:CDS 45.0%
TGCAGTTGAAGAAGGAAGGA+GGG + chr8.3:39406596-39406615 None:intergenic 45.0%
TTGGTGCAGTTGAAGAAGGA+AGG + chr8.3:39406600-39406619 None:intergenic 45.0%
! CTCACTATTGACTGTGGCTT+TGG - chr8.3:39406552-39406571 MS.gene053311:intron 45.0%
! TGGGAGGAAGGAGAGTATAT+GGG + chr8.3:39406244-39406263 None:intergenic 45.0%
! TGTGATGGAGTATCAGTGTC+CGG - chr8.3:39406785-39406804 MS.gene053311:intron 45.0%
! TTGGGAGGAAGGAGAGTATA+TGG + chr8.3:39406245-39406264 None:intergenic 45.0%
!! CATGGAGGTGTTTGAGCATA+GGG + chr8.3:39407277-39407296 None:intergenic 45.0%
!! TATGAAGCACCGACACTTCT+CGG - chr8.3:39406689-39406708 MS.gene053311:intron 45.0%
!! TCATGGAGGTGTTTGAGCAT+AGG + chr8.3:39407278-39407297 None:intergenic 45.0%
!! TTCGGAACTGTTGTTGGCTT+TGG + chr8.3:39407736-39407755 None:intergenic 45.0%
AACACACACTGCAGTCTTGC+TGG - chr8.3:39406890-39406909 MS.gene053311:CDS 50.0%
AAGGAGGGAGGAGAACTAGT+TGG + chr8.3:39406581-39406600 None:intergenic 50.0%
ACGGAACTTTCGAATGTCGC+GGG + chr8.3:39406436-39406455 None:intergenic 50.0%
AGGAGGGAGGAGAACTAGTT+GGG + chr8.3:39406580-39406599 None:intergenic 50.0%
AGTTGAAGAAGGAAGGAGGG+AGG + chr8.3:39406593-39406612 None:intergenic 50.0%
CATCGACGGCATAGACATTG+TGG - chr8.3:39406994-39407013 MS.gene053311:CDS 50.0%
CATTGGCAACTTGCAGACTG+TGG - chr8.3:39407202-39407221 MS.gene053311:CDS 50.0%
CGGAGATACTGCATCTGAGA+CGG + chr8.3:39406455-39406474 None:intergenic 50.0%
CTCGTTTCCCTCATGTAGGT+AGG - chr8.3:39406621-39406640 MS.gene053311:CDS 50.0%
GAAAATCGACCCTGGCATTG+TGG - chr8.3:39406970-39406989 MS.gene053311:CDS 50.0%
GACGAGAGTCATAGTCGCAA+CGG + chr8.3:39406277-39406296 None:intergenic 50.0%
GACGGAACTTTCGAATGTCG+CGG + chr8.3:39406437-39406456 None:intergenic 50.0%
GTGCAGTTGAAGAAGGAAGG+AGG + chr8.3:39406597-39406616 None:intergenic 50.0%
GTTCCTCCTACAAGCAGCAA+GGG + chr8.3:39407690-39407709 None:intergenic 50.0%
TTCGAATGTCGCGGGATCTA+CGG + chr8.3:39406428-39406447 None:intergenic 50.0%
! ACGCCTAATCCGAGAAGTGT+CGG + chr8.3:39406701-39406720 None:intergenic 50.0%
! TTGTGTCCCTTGCTGCTTGT+AGG - chr8.3:39407681-39407700 MS.gene053311:CDS 50.0%
ACGGATACGGACATGGACAC+CGG + chr8.3:39406807-39406826 None:intergenic 55.0%
CACATATCGACGCATTCGCC+GGG + chr8.3:39406492-39406511 None:intergenic 55.0%
CATAGTCGCAACGGAGGTTC+TGG + chr8.3:39406268-39406287 None:intergenic 55.0%
CCACATATCGACGCATTCGC+CGG + chr8.3:39406493-39406512 None:intergenic 55.0%
CCTGGCATTGTGGACATCGA+CGG - chr8.3:39406980-39406999 MS.gene053311:CDS 55.0%
CCTGTAGATGCTCCCAAAGG+TGG + chr8.3:39406314-39406333 None:intergenic 55.0%
CGTCGATGTCCACAATGCCA+GGG + chr8.3:39406982-39407001 None:intergenic 55.0%
GACTTCGACGACGACATGAG+CGG - chr8.3:39406339-39406358 MS.gene053311:CDS 55.0%
GAGAGTCATAGTCGCAACGG+AGG + chr8.3:39406274-39406293 None:intergenic 55.0%
GGTTCCTCCTACAAGCAGCA+AGG + chr8.3:39407691-39407710 None:intergenic 55.0%
TGACACGTGACAGATCCCCT+TGG + chr8.3:39407940-39407959 None:intergenic 55.0%
TGGCAACTTGCAGACTGTGG+AGG - chr8.3:39407205-39407224 MS.gene053311:CDS 55.0%
TTCTTGAAGGTGGGGCCCAA+TGG + chr8.3:39406526-39406545 None:intergenic 55.0%
! CGGCGAATGCGTCGATATGT+GGG - chr8.3:39406491-39406510 MS.gene053311:intron 55.0%
! GCACCGACACTTCTCGGATT+AGG - chr8.3:39406695-39406714 MS.gene053311:intron 55.0%
! TCGCAACGGAGGTTCTGGTT+GGG + chr8.3:39406263-39406282 None:intergenic 55.0%
! TGTCCCTTGCTGCTTGTAGG+AGG - chr8.3:39407684-39407703 MS.gene053311:CDS 55.0%
! TTCTCGGATTAGGCGTGTCC+CGG - chr8.3:39406705-39406724 MS.gene053311:intron 55.0%
!! CCACCTTTGGGAGCATCTAC+AGG - chr8.3:39406311-39406330 MS.gene053311:CDS 55.0%
AATGTCGCGGGATCTACGGC+GGG + chr8.3:39406424-39406443 None:intergenic 60.0%
ATGTGGGTCCGTGCTGCCAT+TGG - chr8.3:39406507-39406526 MS.gene053311:intron 60.0%
CCGTCGATGTCCACAATGCC+AGG + chr8.3:39406983-39407002 None:intergenic 60.0%
CGACGACATGAGCGGTAACC+AGG - chr8.3:39406347-39406366 MS.gene053311:CDS 60.0%
GAATGTCGCGGGATCTACGG+CGG + chr8.3:39406425-39406444 None:intergenic 60.0%
GATTAGGCGTGTCCCGGTGT+TGG - chr8.3:39406711-39406730 MS.gene053311:intron 60.0%
GCTCCCAAAGGTGGAGCCAA+CGG + chr8.3:39406305-39406324 None:intergenic 60.0%
GTGTCGGACGTGTCCAACAC+CGG + chr8.3:39406727-39406746 None:intergenic 60.0%
TGACTCTCGTCTCTCGCCGT+TGG - chr8.3:39406286-39406305 MS.gene053311:CDS 60.0%
TGTCGGACGTGTCCAACACC+GGG + chr8.3:39406726-39406745 None:intergenic 60.0%
TGTGGGTCCGTGCTGCCATT+GGG - chr8.3:39406508-39406527 MS.gene053311:intron 60.0%
! CAACGGAGGTTCTGGTTGGG+AGG + chr8.3:39406260-39406279 None:intergenic 60.0%
! CCGGCGAATGCGTCGATATG+TGG - chr8.3:39406490-39406509 MS.gene053311:intron 60.0%
! GGAGGTTCTGGTTGGGAGGA+AGG + chr8.3:39406256-39406275 None:intergenic 60.0%
! GTCGCAACGGAGGTTCTGGT+TGG + chr8.3:39406264-39406283 None:intergenic 60.0%
!! TCGCCGTTGGCTCCACCTTT+GGG - chr8.3:39406299-39406318 MS.gene053311:CDS 60.0%
!! CTCGCCGTTGGCTCCACCTT+TGG - chr8.3:39406298-39406317 MS.gene053311:CDS 65.0%
GGTGGGGCCCAATGGCAGCA+CGG + chr8.3:39406518-39406537 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 39406144 39407990 39406144 ID=MS.gene053311
chr8.3 mRNA 39406144 39407990 39406144 ID=MS.gene053311.t1;Parent=MS.gene053311
chr8.3 exon 39407500 39407990 39407500 ID=MS.gene053311.t1.exon1;Parent=MS.gene053311.t1
chr8.3 CDS 39407500 39407990 39407500 ID=cds.MS.gene053311.t1;Parent=MS.gene053311.t1
chr8.3 exon 39407122 39407230 39407122 ID=MS.gene053311.t1.exon2;Parent=MS.gene053311.t1
chr8.3 CDS 39407122 39407230 39407122 ID=cds.MS.gene053311.t1;Parent=MS.gene053311.t1
chr8.3 exon 39406791 39407027 39406791 ID=MS.gene053311.t1.exon3;Parent=MS.gene053311.t1
chr8.3 CDS 39406791 39407027 39406791 ID=cds.MS.gene053311.t1;Parent=MS.gene053311.t1
chr8.3 exon 39406554 39406703 39406554 ID=MS.gene053311.t1.exon4;Parent=MS.gene053311.t1
chr8.3 CDS 39406554 39406703 39406554 ID=cds.MS.gene053311.t1;Parent=MS.gene053311.t1
chr8.3 exon 39406144 39406431 39406144 ID=MS.gene053311.t1.exon5;Parent=MS.gene053311.t1
chr8.3 CDS 39406144 39406431 39406144 ID=cds.MS.gene053311.t1;Parent=MS.gene053311.t1
Gene Sequence

>MS.gene053311

ATGGCAACGGCTGTAAGCTCTAAACGACAGAAAGAAGTAGAAAACGACACTGATGAAGATACCGATAGATTTAGTACTCTACCGGACTCTCTTCTTTCCCATATACTCTCCTTCCTCCCAACCAGAACCTCCGTTGCGACTATGACTCTCGTCTCTCGCCGTTGGCTCCACCTTTGGGAGCATCTACAGGTTTTCGACTTCGACGACGACATGAGCGGTAACCAGGAACTGCACAAAATTTTCAGAAGGTTTGCATTTTTCGTGAATGCTGTTCTTTCCCGCCGTAGATCCCGCGACATTCGAAAGTTCCGTCTCAGATGCAGTATCTCCGATTTTTACAACTTTCCCGGCGAATGCGTCGATATGTGGGTCCGTGCTGCCATTGGGCCCCACCTTCAAGAACTTGATCTCACTATTGACTGTGGCTTTGGAGCCCAACTAGTTCTCCTCCCTCCTTCCTTCTTCAACTGCACCAATCTCGTTTCCCTCATGTAGGTAGGTTATTTCATTCTAATGCCACCATCACAAAATTGATCTTCATTATCTATGAAGCACCGACACTTCTCGGATTAGGCGTGTCCCGGTGTTGGACACGTCCGACACGACACTTAATTATGTAATTTTTTTTCCAAATTATTAGTTGTGATGGAGTATCAGTGTCCGGTGTCCATGTCCGTATCCGTGATATACAACTTTTTTAAAGTAACATATATAGAATGTTATTTAACATGTCTTTTTGATTTGATAACACACACTGCAGTCTTGCTGGCTCAATCCTTGTCAAGATTCAACATTCTTCAGTTCATTTTCCGTCACTCAAGATGTTGAAAATCGACCCTGGCATTGTGGACATCGACGGCATAGACATTGTGGATCCACTAATTGACTTTCTCTCTGGCTGTCCCATACTTGAAACTTTTGATACTTTATTTGATTGCTCATTCTTGACCACCTTCCTCCAAGAGATTAAAATTAACCGCTACAAATTTCTCTTGGACTTGTCTTCAAATAGATGCTGATTGGCTTGATGTAAAGTACGGTAAAACTACGTTAGGTATCATTGGCAACTTGCAGACTGTGGAGGAAGCATATCTTGATCTATTTTCCCTGCGTGAAAGTAAATTTGTCGACCCTATGCTCAAACACCTCCATGATCAAAAACATGATTTACATCTACTTTTGCGTCATTCGAAAACAAAGGTGAAGTTTTATTATTATGATTTATATGTATCTATTTGTATATGTAGTTTTGTACTAATATTGCGCAATTATTTGTTATCTGTACAGTGGCCACTTCACACCCCTATTCTAAACCATCCCGAATTTTGCAATTTACATCATCTAAAGTTCATTCTTCCCTGCTTCAACATGAATCTTTTGATCAACGTGCTTGAGAAATGCCATATGCTTCAAGTTCTCATCATTCAGAGCAGCAAGGTTTGTTAAGTTCTCAAGGTTAATTAAATCAATTTTTGGTCTGTCTATGTATGCACGTATATGTATCTATCTATACACACTCAAATATAACTTTGAGTCGTTGTGTCCCTTGCTGCTTGTAGGAGGAACCATCACCTTTAAGAACATGGGATCCAAAGCCAACAACAGTTCCGAAATGTCTTGAATCCCACCTGACCTGTATTCACATAGAAGGATATCAAGGATTTGAAGATGAGCTGGCATTTACAGAATATATTTTGCGTCACGGGCTTGTTTTGCACACAGTGCTTATTTTTTTCAACACTTCAATAGACCTAACTAACAAGTATCGTTCTCTCAAAAGATTAACCGATATACCAAGGGGATCTGTCACGTGTCAACTGAAATTTGACTCAACCGTGTCTCCTTAA
Protein sequence

>MS.gene053311.t1

MATAVSSKRQKEVENDTDEDTDRFSTLPDSLLSHILSFLPTRTSVATMTLVSRRWLHLWEHLQVFDFDDDMSGNQELHKIFRRFAFFVNAVLSRRRSRDIRKFRLRCSISDFYNFPGECVDMWVRAAIGPHLQELDLTIDCGFGAQLVLLPPSFFNCTNLVSLILAGSILVKIQHSSVHFPSLKMLKIDPGIVDIDGIDIRLKLTATNFSWTCLQIDADWLDVKYGKTTLGIIGNLQTVEEAYLDLFSLRESKFVDPMLKHLHDQKHDLHLLLRHSKTKWPLHTPILNHPEFCNLHHLKFILPCFNMNLLINVLEKCHMLQVLIIQSSKEEPSPLRTWDPKPTTVPKCLESHLTCIHIEGYQGFEDELAFTEYILRHGLVLHTVLIFFNTSIDLTNKYRSLKRLTDIPRGSVTCQLKFDSTVSP