AlfalfaGEDB | MS.gene053311

M. sativa cultivar XinJiangDaYe / MS.gene053311

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF08387.13 PF12937.10

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053311.t1	RHN60394.1	66.5	480	104	7	1	424	1	479	7.30E-166	593.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053311.t1	Q9FM89	26.9	442	254	17	22	419	6	422	2.3e-19	98.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053311.t1	A0A392M1I8	72.4	463	79	5	6	424	7	464	5.6e-184	653.3

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene002112	MS.gene053311	PPI
MS.gene003456	MS.gene053311	PPI
MS.gene004045	MS.gene053311	PPI
MS.gene005794	MS.gene053311	PPI
MS.gene005795	MS.gene053311	PPI
MS.gene006692	MS.gene053311	PPI
MS.gene006725	MS.gene053311	PPI
MS.gene006727	MS.gene053311	PPI
MS.gene006728	MS.gene053311	PPI
MS.gene006730	MS.gene053311	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053311.t1	MTR_8g469020	61.504	452	125	4	21	424	6	456	0.0	538
MS.gene053311.t1	MTR_8g058965	63.261	460	120	4	11	423	3	460	0.0	533
MS.gene053311.t1	MTR_4g050250	63.981	422	93	7	1	365	1	420	6.43e-175	496
MS.gene053311.t1	MTR_8g062010	56.293	437	145	9	22	423	9	434	7.00e-154	442
MS.gene053311.t1	MTR_8g070950	56.081	444	141	10	19	424	12	439	6.14e-148	427
MS.gene053311.t1	MTR_8g062310	51.648	455	167	9	1	424	1	433	2.64e-143	416
MS.gene053311.t1	MTR_8g062080	51.204	457	174	11	4	423	5	449	2.56e-141	411
MS.gene053311.t1	MTR_8g062300	46.817	487	172	13	8	423	11	481	1.62e-124	369
MS.gene053311.t1	MTR_2g083840	43.326	457	205	9	8	419	10	457	5.21e-97	298
MS.gene053311.t1	MTR_8g468810	41.845	466	213	12	6	419	4	463	4.71e-96	296

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053311.t1	AT5G56420	26.471	442	256	15	22	419	6	422	1.05e-21	97.1
MS.gene053311.t1	AT5G56420	26.471	442	256	15	22	419	6	422	1.05e-21	97.1
MS.gene053311.t1	AT5G56420	26.471	442	256	15	22	419	6	422	1.05e-21	97.1
MS.gene053311.t1	AT2G26030	24.034	466	256	17	20	416	2	438	5.53e-20	92.0
MS.gene053311.t1	AT2G26030	24.034	466	256	17	20	416	2	438	5.53e-20	92.0
MS.gene053311.t1	AT2G26030	23.605	466	263	17	20	416	2	443	1.58e-19	90.9
MS.gene053311.t1	AT4G10400	35.329	167	98	5	22	188	2	158	6.87e-18	85.5
MS.gene053311.t1	AT4G10400	35.329	167	98	5	22	188	2	158	6.87e-18	85.5
MS.gene053311.t1	AT4G10400	35.329	167	98	5	22	188	2	158	6.87e-18	85.5
MS.gene053311.t1	AT4G09920	34.731	167	99	5	22	188	2	158	1.40e-17	83.2

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Find 103 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTACGTTAGGTATCAT+TGG	0.457269	8.3:-39406927	MS.gene053311:CDS
AAAAGATTCATGTTGAAGCA+GGG	0.613622	8.3:+39406612	None:intergenic
AAACCTGTAGATGCTCCCAA+AGG	0.675107	8.3:+39407798	None:intergenic
AAAGATTAACCGATATACCA+AGG	0.607874	8.3:-39406192	MS.gene053311:CDS
AACACACACTGCAGTCTTGC+TGG	0.372624	8.3:-39407222	MS.gene053311:intron
AACCATCACCTTTAAGAACA+TGG	0.545612	8.3:-39406405	MS.gene053311:CDS
AAGAATGTTGAATCTTGACA+AGG	0.659316	8.3:+39407193	None:intergenic
AAGATGTTGAAAATCGACCC+TGG	0.690890	8.3:-39407150	MS.gene053311:CDS
AAGATTAACCGATATACCAA+GGG	0.609206	8.3:-39406191	MS.gene053311:CDS
AAGGAGGGAGGAGAACTAGT+TGG	0.461653	8.3:+39407534	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTACGTTAGGTATCAT+TGG	-	chr8.3:39407185-39407204	MS.gene053311:CDS	30.0%
AAAAGATTCATGTTGAAGCA+GGG	+	chr8.3:39407503-39407522	None:intergenic	30.0%
AAAGATTAACCGATATACCA+AGG	-	chr8.3:39407920-39407939	MS.gene053311:CDS	30.0%
AAAGTTGTATATCACGGATA+CGG	+	chr8.3:39406820-39406839	None:intergenic	30.0%
AAGAATGTTGAATCTTGACA+AGG	+	chr8.3:39406922-39406941	None:intergenic	30.0%
AAGATTAACCGATATACCAA+GGG	-	chr8.3:39407921-39407940	MS.gene053311:CDS	30.0%
ATATCCTTCTATGTGAATAC+AGG	+	chr8.3:39407779-39407798	None:intergenic	30.0%
ATTCACATAGAAGGATATCA+AGG	-	chr8.3:39407781-39407800	MS.gene053311:CDS	30.0%
CTCCATCACAACTAATAATT+TGG	+	chr8.3:39406775-39406794	None:intergenic	30.0%
AAGTACGGTAAAACTACGTT+AGG	-	chr8.3:39407176-39407195	MS.gene053311:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	39406144	39407990	39406144	ID=MS.gene053311
chr8.3	mRNA	39406144	39407990	39406144	ID=MS.gene053311.t1;Parent=MS.gene053311
chr8.3	exon	39407500	39407990	39407500	ID=MS.gene053311.t1.exon1;Parent=MS.gene053311.t1
chr8.3	CDS	39407500	39407990	39407500	ID=cds.MS.gene053311.t1;Parent=MS.gene053311.t1
chr8.3	exon	39407122	39407230	39407122	ID=MS.gene053311.t1.exon2;Parent=MS.gene053311.t1
chr8.3	CDS	39407122	39407230	39407122	ID=cds.MS.gene053311.t1;Parent=MS.gene053311.t1
chr8.3	exon	39406791	39407027	39406791	ID=MS.gene053311.t1.exon3;Parent=MS.gene053311.t1
chr8.3	CDS	39406791	39407027	39406791	ID=cds.MS.gene053311.t1;Parent=MS.gene053311.t1
chr8.3	exon	39406554	39406703	39406554	ID=MS.gene053311.t1.exon4;Parent=MS.gene053311.t1
chr8.3	CDS	39406554	39406703	39406554	ID=cds.MS.gene053311.t1;Parent=MS.gene053311.t1
chr8.3	exon	39406144	39406431	39406144	ID=MS.gene053311.t1.exon5;Parent=MS.gene053311.t1
chr8.3	CDS	39406144	39406431	39406144	ID=cds.MS.gene053311.t1;Parent=MS.gene053311.t1

Gene Sequence

>MS.gene053311

ATGGCAACGGCTGTAAGCTCTAAACGACAGAAAGAAGTAGAAAACGACACTGATGAAGATACCGATAGATTTAGTACTCTACCGGACTCTCTTCTTTCCCATATACTCTCCTTCCTCCCAACCAGAACCTCCGTTGCGACTATGACTCTCGTCTCTCGCCGTTGGCTCCACCTTTGGGAGCATCTACAGGTTTTCGACTTCGACGACGACATGAGCGGTAACCAGGAACTGCACAAAATTTTCAGAAGGTTTGCATTTTTCGTGAATGCTGTTCTTTCCCGCCGTAGATCCCGCGACATTCGAAAGTTCCGTCTCAGATGCAGTATCTCCGATTTTTACAACTTTCCCGGCGAATGCGTCGATATGTGGGTCCGTGCTGCCATTGGGCCCCACCTTCAAGAACTTGATCTCACTATTGACTGTGGCTTTGGAGCCCAACTAGTTCTCCTCCCTCCTTCCTTCTTCAACTGCACCAATCTCGTTTCCCTCATGTAGGTAGGTTATTTCATTCTAATGCCACCATCACAAAATTGATCTTCATTATCTATGAAGCACCGACACTTCTCGGATTAGGCGTGTCCCGGTGTTGGACACGTCCGACACGACACTTAATTATGTAATTTTTTTTCCAAATTATTAGTTGTGATGGAGTATCAGTGTCCGGTGTCCATGTCCGTATCCGTGATATACAACTTTTTTAAAGTAACATATATAGAATGTTATTTAACATGTCTTTTTGATTTGATAACACACACTGCAGTCTTGCTGGCTCAATCCTTGTCAAGATTCAACATTCTTCAGTTCATTTTCCGTCACTCAAGATGTTGAAAATCGACCCTGGCATTGTGGACATCGACGGCATAGACATTGTGGATCCACTAATTGACTTTCTCTCTGGCTGTCCCATACTTGAAACTTTTGATACTTTATTTGATTGCTCATTCTTGACCACCTTCCTCCAAGAGATTAAAATTAACCGCTACAAATTTCTCTTGGACTTGTCTTCAAATAGATGCTGATTGGCTTGATGTAAAGTACGGTAAAACTACGTTAGGTATCATTGGCAACTTGCAGACTGTGGAGGAAGCATATCTTGATCTATTTTCCCTGCGTGAAAGTAAATTTGTCGACCCTATGCTCAAACACCTCCATGATCAAAAACATGATTTACATCTACTTTTGCGTCATTCGAAAACAAAGGTGAAGTTTTATTATTATGATTTATATGTATCTATTTGTATATGTAGTTTTGTACTAATATTGCGCAATTATTTGTTATCTGTACAGTGGCCACTTCACACCCCTATTCTAAACCATCCCGAATTTTGCAATTTACATCATCTAAAGTTCATTCTTCCCTGCTTCAACATGAATCTTTTGATCAACGTGCTTGAGAAATGCCATATGCTTCAAGTTCTCATCATTCAGAGCAGCAAGGTTTGTTAAGTTCTCAAGGTTAATTAAATCAATTTTTGGTCTGTCTATGTATGCACGTATATGTATCTATCTATACACACTCAAATATAACTTTGAGTCGTTGTGTCCCTTGCTGCTTGTAGGAGGAACCATCACCTTTAAGAACATGGGATCCAAAGCCAACAACAGTTCCGAAATGTCTTGAATCCCACCTGACCTGTATTCACATAGAAGGATATCAAGGATTTGAAGATGAGCTGGCATTTACAGAATATATTTTGCGTCACGGGCTTGTTTTGCACACAGTGCTTATTTTTTTCAACACTTCAATAGACCTAACTAACAAGTATCGTTCTCTCAAAAGATTAACCGATATACCAAGGGGATCTGTCACGTGTCAACTGAAATTTGACTCAACCGTGTCTCCTTAA

Protein sequence

>MS.gene053311.t1

MATAVSSKRQKEVENDTDEDTDRFSTLPDSLLSHILSFLPTRTSVATMTLVSRRWLHLWEHLQVFDFDDDMSGNQELHKIFRRFAFFVNAVLSRRRSRDIRKFRLRCSISDFYNFPGECVDMWVRAAIGPHLQELDLTIDCGFGAQLVLLPPSFFNCTNLVSLILAGSILVKIQHSSVHFPSLKMLKIDPGIVDIDGIDIRLKLTATNFSWTCLQIDADWLDVKYGKTTLGIIGNLQTVEEAYLDLFSLRESKFVDPMLKHLHDQKHDLHLLLRHSKTKWPLHTPILNHPEFCNLHHLKFILPCFNMNLLINVLEKCHMLQVLIIQSSKEEPSPLRTWDPKPTTVPKCLESHLTCIHIEGYQGFEDELAFTEYILRHGLVLHTVLIFFNTSIDLTNKYRSLKRLTDIPRGSVTCQLKFDSTVSP