AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34704

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34704.t1 MTR_8g467800 93.458 321 21 0 1 321 76 396 0.0 582
MS.gene34704.t1 MTR_8g467800 93.162 234 16 0 1 234 76 309 7.78e-145 412
MS.gene34704.t1 MTR_8g062270 59.627 322 124 3 1 320 81 398 2.96e-120 353
MS.gene34704.t1 MTR_8g468840 58.154 325 125 5 1 321 76 393 4.54e-116 343
MS.gene34704.t1 MTR_5g092960 57.751 329 122 7 3 321 84 405 1.22e-109 327
MS.gene34704.t1 MTR_8g468810 52.727 330 129 10 3 321 88 401 1.89e-100 303
MS.gene34704.t1 MTR_4g032730 50.733 341 138 7 1 321 79 409 7.74e-98 296
MS.gene34704.t1 MTR_5g092960 57.534 292 110 6 3 284 84 371 9.22e-94 284
MS.gene34704.t1 MTR_2g083840 52.454 326 137 7 2 321 82 395 5.01e-93 284
MS.gene34704.t1 MTR_8g468810 53.082 292 119 8 3 284 88 371 6.61e-89 271
MS.gene34704.t1 MTR_8g468760 51.852 324 115 9 1 321 74 359 3.10e-88 270
MS.gene34704.t1 MTR_8g058970 49.138 348 134 12 3 321 82 415 3.10e-83 259
MS.gene34704.t1 MTR_8g468810 57.269 227 87 5 3 226 88 307 6.23e-74 231
MS.gene34704.t1 MTR_8g468810 57.269 227 87 5 3 226 88 307 7.01e-74 231
MS.gene34704.t1 MTR_8g468810 57.269 227 87 5 3 226 88 307 1.87e-73 230
MS.gene34704.t1 MTR_8g445780 44.795 317 151 10 13 321 77 377 1.66e-66 214
MS.gene34704.t1 MTR_8g014260 41.566 332 167 11 2 321 75 391 2.58e-64 210
MS.gene34704.t1 MTR_4g050250 40.237 338 173 10 3 321 87 414 1.48e-57 191
MS.gene34704.t1 MTR_8g058965 40.896 335 171 9 2 321 74 396 2.31e-56 189
MS.gene34704.t1 MTR_8g469020 36.982 338 179 9 3 321 69 391 3.52e-54 183
MS.gene34704.t1 MTR_8g062310 37.224 317 166 9 3 318 81 365 1.16e-48 168
MS.gene34704.t1 MTR_8g070950 36.826 334 162 13 2 321 76 374 2.09e-43 154
MS.gene34704.t1 MTR_8g062080 35.843 332 172 12 3 321 82 385 1.57e-39 144
MS.gene34704.t1 MTR_8g014160 42.917 240 123 7 2 234 106 338 4.27e-38 140
MS.gene34704.t1 MTR_8g062010 34.568 324 184 8 2 321 71 370 4.45e-37 137
MS.gene34704.t1 MTR_8g062300 33.708 356 179 11 2 321 83 417 8.07e-37 137
MS.gene34704.t1 MTR_4g024745 39.357 249 131 8 77 321 10 242 1.33e-36 133
MS.gene34704.t1 MTR_4g024710 38.367 245 135 7 79 321 58 288 2.78e-36 134
MS.gene34704.t1 MTR_8g014210 43.169 183 82 5 145 321 6 172 4.89e-32 119
MS.gene34704.t1 MTR_8g467790 31.832 333 158 12 2 321 213 489 9.53e-32 124
MS.gene34704.t1 MTR_5g084070 29.503 322 205 10 2 321 66 367 1.76e-20 91.7
MS.gene34704.t1 MTR_8g077460 36.585 164 92 5 2 157 70 229 5.07e-20 89.7
MS.gene34704.t1 MTR_8g077460 31.638 177 100 8 151 321 155 316 3.30e-13 70.1
MS.gene34704.t1 MTR_4g113810 38.562 153 77 6 38 181 18 162 8.58e-19 82.4
MS.gene34704.t1 MTR_4g056070 30.178 338 194 13 5 321 108 424 2.61e-18 85.5
MS.gene34704.t1 MTR_4g056040 29.762 336 196 13 6 321 99 414 8.14e-18 84.3
MS.gene34704.t1 MTR_1g038710 28.529 333 210 12 6 321 79 400 9.55e-17 80.9
MS.gene34704.t1 MTR_4g056020 30.422 332 199 13 6 321 69 384 2.07e-16 80.1
MS.gene34704.t1 MTR_4g055990 32.180 289 169 12 5 278 105 381 5.02e-16 79.0
MS.gene34704.t1 MTR_4g055990 32.639 288 169 12 5 278 105 381 5.15e-16 79.0
MS.gene34704.t1 MTR_4g056030 29.969 327 205 13 6 320 69 383 2.36e-15 76.6
MS.gene34704.t1 MTR_5g039950 28.975 283 151 12 13 278 77 326 2.96e-15 76.3
MS.gene34704.t1 MTR_8g014290 48.810 84 37 3 2 79 77 160 5.61e-15 72.0
MS.gene34704.t1 MTR_5g021550 49.398 83 36 3 2 78 80 162 1.06e-14 71.2
MS.gene34704.t1 MTR_5g080710 28.615 325 207 11 2 321 89 393 2.68e-14 73.6
MS.gene34704.t1 MTR_2g086020 41.667 108 56 4 4 105 32 138 2.77e-14 72.8
MS.gene34704.t1 MTR_3g107120 26.224 286 147 8 38 321 114 337 1.71e-11 65.1
MS.gene34704.t1 MTR_4g055580 27.492 331 198 11 11 320 201 510 5.57e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34704.t1 AT1G16930 28.571 336 202 13 3 321 70 384 1.19e-19 89.4
MS.gene34704.t1 AT1G16930 28.571 336 202 13 3 321 70 384 1.19e-19 89.4
MS.gene34704.t1 AT1G16930 28.571 336 202 13 3 321 70 384 1.19e-19 89.4
MS.gene34704.t1 AT4G13985 27.526 287 190 6 6 279 85 366 1.61e-16 80.5
MS.gene34704.t1 AT4G26340 32.258 279 161 14 42 312 93 351 4.48e-16 79.0
MS.gene34704.t1 AT1G13780 31.387 274 148 11 65 318 138 391 7.27e-16 78.2
MS.gene34704.t1 AT1G78750 28.094 299 186 10 2 280 79 368 1.15e-15 77.8
MS.gene34704.t1 AT1G78750 28.094 299 186 10 2 280 79 368 1.15e-15 77.8
MS.gene34704.t1 AT1G69630 29.703 303 176 14 2 280 72 361 2.12e-15 77.0
MS.gene34704.t1 AT3G44060 29.333 300 175 11 2 282 57 338 9.16e-15 75.1
MS.gene34704.t1 AT5G22700 29.568 301 167 12 42 318 97 376 1.01e-14 74.7
MS.gene34704.t1 AT5G22700 29.568 301 167 12 42 318 97 376 1.01e-14 74.7
MS.gene34704.t1 AT5G22700 29.568 301 167 12 42 318 97 376 1.01e-14 74.7
MS.gene34704.t1 AT5G22700 29.568 301 167 12 42 318 97 376 1.01e-14 74.7
MS.gene34704.t1 AT5G22700 29.568 301 167 12 42 318 134 413 1.56e-14 74.3
MS.gene34704.t1 AT5G53635 25.633 316 205 10 19 318 71 372 1.80e-14 73.9
MS.gene34704.t1 AT5G44970 29.020 255 157 12 42 279 46 293 2.55e-14 73.6
MS.gene34704.t1 AT1G06630 29.097 299 192 11 2 291 75 362 2.87e-14 73.6
MS.gene34704.t1 AT1G06630 29.097 299 192 11 2 291 75 362 2.87e-14 73.6
MS.gene34704.t1 AT1G06630 29.097 299 192 11 2 291 75 362 2.87e-14 73.6
MS.gene34704.t1 AT1G06630 29.097 299 192 11 2 291 75 362 2.87e-14 73.6
MS.gene34704.t1 AT1G66290 25.982 331 196 11 13 321 92 395 3.15e-14 73.6
MS.gene34704.t1 AT1G06630 28.523 298 195 10 2 291 84 371 3.38e-14 73.2
MS.gene34704.t1 AT1G06630 29.097 299 192 11 2 291 75 362 4.09e-14 73.2
MS.gene34704.t1 AT1G06630 28.671 286 186 10 2 279 75 350 7.57e-14 72.0
MS.gene34704.t1 AT1G06630 28.671 286 186 10 2 279 75 350 7.57e-14 72.0
MS.gene34704.t1 AT1G66300 27.243 301 192 10 2 284 83 374 7.81e-14 72.4
MS.gene34704.t1 AT5G27750 28.881 277 152 9 65 315 133 390 1.47e-13 71.6
MS.gene34704.t1 AT5G22660 29.304 273 156 10 32 279 94 354 3.61e-13 70.1
MS.gene34704.t1 AT2G26030 26.608 342 199 16 2 315 57 374 3.68e-13 70.1
MS.gene34704.t1 AT2G26030 26.608 342 199 16 2 315 57 374 3.68e-13 70.1
MS.gene34704.t1 AT5G27750 30.508 236 138 7 65 279 133 363 9.02e-13 68.6
MS.gene34704.t1 AT1G78760 25.526 333 216 13 2 321 70 383 1.06e-12 68.9
MS.gene34704.t1 AT1G66310 27.740 292 190 9 2 280 73 356 2.24e-12 67.8
MS.gene34704.t1 AT3G59180 27.609 297 187 13 2 279 37 324 2.40e-12 67.8
MS.gene34704.t1 AT1G51370 26.389 288 180 11 41 318 108 373 3.51e-12 67.4
MS.gene34704.t1 AT1G51370 26.389 288 180 11 41 318 108 373 3.65e-12 67.0
MS.gene34704.t1 AT3G50710 27.517 298 184 13 36 321 89 366 5.40e-12 66.6
MS.gene34704.t1 AT3G59210 27.946 297 186 12 2 279 70 357 5.74e-12 66.6
MS.gene34704.t1 AT3G59210 27.946 297 186 12 2 279 70 357 5.74e-12 66.6
MS.gene34704.t1 AT3G59210 27.946 297 186 12 2 279 70 357 5.74e-12 66.6
MS.gene34704.t1 AT3G59210 27.946 297 186 12 2 279 70 357 5.74e-12 66.6
MS.gene34704.t1 AT3G59210 27.946 297 186 12 2 279 70 357 5.74e-12 66.6
MS.gene34704.t1 AT2G26030 27.385 325 184 16 19 315 1 301 8.64e-12 65.9
MS.gene34704.t1 AT5G35995 27.181 298 178 10 2 278 61 340 9.81e-12 65.9
MS.gene34704.t1 AT5G35995 27.181 298 178 10 2 278 61 340 9.81e-12 65.9
MS.gene34704.t1 AT3G59200 28.525 305 179 14 2 279 70 362 1.74e-11 64.7
MS.gene34704.t1 AT3G58920 26.498 317 179 12 6 279 59 364 2.32e-11 64.7
MS.gene34704.t1 AT3G62440 24.516 310 195 9 2 278 55 358 2.34e-11 64.7
MS.gene34704.t1 AT3G59230 26.100 341 210 13 2 313 77 404 2.53e-11 64.7
MS.gene34704.t1 AT3G59200 28.990 307 175 15 2 279 70 362 2.65e-11 64.7
MS.gene34704.t1 AT5G22730 26.974 304 175 13 39 315 118 401 2.75e-11 64.7
MS.gene34704.t1 AT5G22730 26.974 304 175 13 39 315 118 401 2.75e-11 64.7
MS.gene34704.t1 AT5G60610 27.365 296 193 10 32 320 78 358 3.21e-11 64.3
MS.gene34704.t1 AT5G60610 27.365 296 193 10 32 320 78 358 3.21e-11 64.3
MS.gene34704.t1 AT1G66320 26.316 304 181 13 6 283 88 374 4.80e-11 63.9
MS.gene34704.t1 AT5G44980 26.706 337 202 16 3 318 62 374 9.53e-11 62.8
MS.gene34704.t1 AT5G44980 26.706 337 202 16 3 318 62 374 9.53e-11 62.8

Find 91 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCTGGATATCCTTCTTTC+TGG 0.084980 8.1:-45657239 MS.gene34704:CDS
AGACAGTTCGGAACATTCTT+AGG 0.267813 8.1:+45656505 None:intergenic
TTCGAATTGCAAGACACTTC+CGG 0.288059 8.1:-45657323 MS.gene34704:CDS
TAAACTAAATGGTGAAATTC+TGG 0.290598 8.1:+45656762 None:intergenic
CGAGCTCGAGATGAAATTCC+TGG 0.308419 8.1:+45658101 None:intergenic
CGATGAATTGGTCAATCTTC+GGG 0.308827 8.1:-45657168 MS.gene34704:CDS
CTGCCCCATTCTTGAAACTT+TGG 0.325782 8.1:-45657216 MS.gene34704:CDS
ACTTTGGAACTTCTTTCCTT+TGG 0.338556 8.1:-45657200 MS.gene34704:CDS
TCGAATTGCAAGACACTTCC+GGG 0.347239 8.1:-45657322 MS.gene34704:CDS
GAGCAAGAGAAGAGAGTGAT+AGG 0.362976 8.1:+45658058 None:intergenic
AGACCTTTAGATAAACTAAA+TGG 0.365198 8.1:+45656751 None:intergenic
CCTTCATTGGAAGTAAATAA+AGG 0.367090 8.1:+45656468 None:intergenic
TTCAAGCTCTTCAAGGAAGA+TGG 0.372549 8.1:+45657143 None:intergenic
GGTTCAACAGATTCACTATC+AGG 0.377584 8.1:+45656966 None:intergenic
GAGTGTGTGTAGAACGCTTC+AGG 0.385157 8.1:+45658163 None:intergenic
ACGATGAATTGGTCAATCTT+CGG 0.387514 8.1:-45657169 MS.gene34704:CDS
GAACTTCTTTCCTTTGGTCT+CGG 0.395783 8.1:-45657194 MS.gene34704:CDS
AGCCAGAAAGAAGGATATCC+AGG 0.404828 8.1:+45657237 None:intergenic
TAGGCTTTGCCGCCCATCCA+TGG 0.408560 8.1:+45656524 None:intergenic
AAACTCCTCCGTGCTCTCTC+TGG 0.416986 8.1:-45656936 MS.gene34704:CDS
AGTTCCAAAGTTTCAAGAAT+GGG 0.417262 8.1:+45657212 None:intergenic
TGGTCTCGGTTACGATGAAT+TGG 0.430953 8.1:-45657180 MS.gene34704:CDS
ATCACTGTTGAGGTTGGGTA+TGG 0.432139 8.1:-45657119 MS.gene34704:CDS
CCTTTATTTACTTCCAATGA+AGG 0.432238 8.1:-45656468 None:intergenic
TTGAAAATCACTGTTGAGGT+TGG 0.440618 8.1:-45657125 MS.gene34704:CDS
AAGAGAAGAGAGTGATAGGA+AGG 0.442672 8.1:+45658062 None:intergenic
AGCCGTTGGGCCCCACCTCC+AGG 0.443057 8.1:-45658119 MS.gene34704:CDS
TCACCATTTAGTTTATCTAA+AGG 0.443116 8.1:-45656754 MS.gene34704:CDS
CACTATCAGGATGAGAAAAT+AGG 0.443164 8.1:+45656979 None:intergenic
AAATTCTGGCATATCTAGAA+TGG 0.447372 8.1:+45656776 None:intergenic
TCCGTAGCTCACGTCACATC+AGG 0.462729 8.1:-45658216 MS.gene34704:CDS
AATTCTGGCATATCTAGAAT+GGG 0.468776 8.1:+45656777 None:intergenic
TGAAAATCACTGTTGAGGTT+GGG 0.475638 8.1:-45657124 MS.gene34704:CDS
GAGCAGGCGCGTCTATTTCA+AGG 0.478426 8.1:+45657090 None:intergenic
CGAGATGAAATTCCTGGAGG+TGG 0.480035 8.1:+45658107 None:intergenic
ATTCATCACCTTCCCATGGA+TGG 0.489968 8.1:-45656537 MS.gene34704:CDS
ATGTGACAGCGGCGAGGCTA+AGG 0.492580 8.1:+45657060 None:intergenic
AAGTTCCAAAGTTTCAAGAA+TGG 0.495558 8.1:+45657211 None:intergenic
TTCCTGGAGGTGGGGCCCAA+CGG 0.495873 8.1:+45658117 None:intergenic
GGTGGGGCCCAACGGCTGTA+CGG 0.499748 8.1:+45658125 None:intergenic
GCTCTAACCTACTCTCTCTC+AGG 0.499774 8.1:-45658039 MS.gene34704:intron
TGTGTAGAACGCTTCAGGTT+CGG 0.501252 8.1:+45658168 None:intergenic
CGTGGATCCGTACAGCCGTT+GGG 0.504745 8.1:-45658132 MS.gene34704:CDS
AGGCTTTGCCGCCCATCCAT+GGG 0.512979 8.1:+45656525 None:intergenic
GTTATGAGAGTTTGTAGCAC+AGG 0.517195 8.1:+45656678 None:intergenic
AGAAAACTCAAGTTGAAAGA+AGG 0.518120 8.1:+45656723 None:intergenic
AGGCTAAGGTACTTGAGAGC+AGG 0.521075 8.1:+45657074 None:intergenic
TTTCTCAATGCTGCTACTAT+CGG 0.523131 8.1:-45657038 MS.gene34704:CDS
TCGCTGAAGGTGATGAAACT+AGG 0.528045 8.1:-45657287 MS.gene34704:CDS
AAGTGTGATATGAGACAGTT+CGG 0.530097 8.1:+45656493 None:intergenic
TAGCTTGGATATGAATTCCC+TGG 0.530498 8.1:-45657255 MS.gene34704:CDS
ACTAGGATACAACTATAGCT+TGG 0.545967 8.1:-45657270 MS.gene34704:CDS
GCACGGAGGAGTTTGAGTAA+AGG 0.547105 8.1:+45656945 None:intergenic
CAGCGATGGTAAACAGATCC+CGG 0.547768 8.1:+45657304 None:intergenic
GAGGACGGCGTTGACGAAAA+CGG 0.549284 8.1:+45658243 None:intergenic
GAATCATCGGTGACTGATGT+TGG 0.554746 8.1:+45656556 None:intergenic
TCCTGATGTGACGTGAGCTA+CGG 0.556544 8.1:+45658215 None:intergenic
GAGATGAAATTCCTGGAGGT+GGG 0.556856 8.1:+45658108 None:intergenic
TTCATCACCTTCCCATGGAT+GGG 0.557135 8.1:-45656536 MS.gene34704:CDS
GTTTAAGTCCAGAGAGAGCA+CGG 0.558504 8.1:+45656928 None:intergenic
AGAGAAGAGAGTGATAGGAA+GGG 0.560462 8.1:+45658063 None:intergenic
AGTTTCATCACCTTCAGCGA+TGG 0.570427 8.1:+45657290 None:intergenic
GAATGGGGCAGCCAGAAAGA+AGG 0.570578 8.1:+45657228 None:intergenic
TCGTGGATCCGTACAGCCGT+TGG 0.571931 8.1:-45658133 MS.gene34704:CDS
TCACTGTTGAGGTTGGGTAT+GGG 0.574478 8.1:-45657118 MS.gene34704:CDS
ACACACACTCAGTCGACTCG+TGG 0.583493 8.1:-45658150 MS.gene34704:CDS
GGAATTTCATCTCGAGCTCG+AGG 0.585969 8.1:-45658098 MS.gene34704:CDS
ATGGGAAGGTGATGAATCAT+CGG 0.591261 8.1:+45656543 None:intergenic
TGAGCTACGGAGAGCGAGGA+CGG 0.597280 8.1:+45658228 None:intergenic
GATCTGTTTACCATCGCTGA+AGG 0.602656 8.1:-45657300 MS.gene34704:CDS
AGATGAAATTCCTGGAGGTG+GGG 0.604196 8.1:+45658109 None:intergenic
GTAATGTACCTCAGTCTCGA+TGG 0.606426 8.1:-45657356 MS.gene34704:intron
ATCACCTTCCCATGGATGGG+CGG 0.611178 8.1:-45656533 MS.gene34704:CDS
GTTCCAAAGTTTCAAGAATG+GGG 0.611352 8.1:+45657213 None:intergenic
TTTGCCGCCCATCCATGGGA+AGG 0.619609 8.1:+45656529 None:intergenic
GATGATTCATCACCTTCCCA+TGG 0.624623 8.1:-45656541 MS.gene34704:CDS
CGGCGAGAACTTGCACAACG+TGG 0.633645 8.1:-45657018 MS.gene34704:CDS
GCTACATAGCTCAACAACAC+AGG 0.633907 8.1:-45656898 MS.gene34704:intron
ATTCTGGCATATCTAGAATG+GGG 0.634602 8.1:+45656778 None:intergenic
TCTCATAACATACGATGACC+AGG 0.635602 8.1:-45656664 MS.gene34704:intron
GAATTTCATCTCGAGCTCGA+GGG 0.650561 8.1:-45658097 MS.gene34704:CDS
TTCTGGCATATCTAGAATGG+GGG 0.653424 8.1:+45656779 None:intergenic
TTCATCGTAACCGAGACCAA+AGG 0.659971 8.1:+45657184 None:intergenic
GCCAGAAAGAAGGATATCCA+GGG 0.663044 8.1:+45657238 None:intergenic
ATGGTTACCTGAGAGAGAGT+AGG 0.663839 8.1:+45658032 None:intergenic
GAGCTTGAAAATCACTGTTG+AGG 0.665933 8.1:-45657129 MS.gene34704:CDS
GACGTGAGCTACGGAGAGCG+AGG 0.671741 8.1:+45658224 None:intergenic
TGAGAAATGTGACAGCGGCG+AGG 0.703379 8.1:+45657054 None:intergenic
AGCATTGAGAAATGTGACAG+CGG 0.715511 8.1:+45657049 None:intergenic
GCTCGAGATGAAATTCCTGG+AGG 0.762394 8.1:+45658104 None:intergenic
TAAGTCCAGAGAGAGCACGG+AGG 0.795867 8.1:+45656931 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTAATCATTTGAAAAA+TGG - chr8.1:45656956-45656975 MS.gene34704:CDS 10.0%
!! TTACATGTATAAATGAAAAT+AGG - chr8.1:45656850-45656869 MS.gene34704:intron 15.0%
!! TTCATTTATACATGTAATTT+GGG + chr8.1:45656847-45656866 None:intergenic 15.0%
!! TTGTAAAATGACATATAATT+TGG - chr8.1:45657296-45657315 MS.gene34704:CDS 15.0%
!! TTTCATTTATACATGTAATT+TGG + chr8.1:45656848-45656867 None:intergenic 15.0%
!!! AATATTAATCATTTGACTTT+TGG + chr8.1:45657119-45657138 None:intergenic 15.0%
!!! TATCAATATTTGGAATATTA+AGG + chr8.1:45656777-45656796 None:intergenic 15.0%
!! AAATGAAAATAGGCTTAAAT+AGG - chr8.1:45656860-45656879 MS.gene34704:intron 20.0%
!! TTACAAGTCATATCAATATT+TGG + chr8.1:45656787-45656806 None:intergenic 20.0%
! AAGATCCAAATAAAAAATGC+AGG + chr8.1:45658118-45658137 None:intergenic 25.0%
! AGACCTTTAGATAAACTAAA+TGG + chr8.1:45657976-45657995 None:intergenic 25.0%
! CAAATGATTAATATTTGCAG+CGG - chr8.1:45657124-45657143 MS.gene34704:CDS 25.0%
! CAAATGATTAATATTTGCAG+TGG + chr8.1:45657143-45657124 None:intergenic 25.0%
! CTCAACAAAAAAACCTAATT+AGG - chr8.1:45657092-45657111 MS.gene34704:CDS 25.0%
! GTTACTAATCAGATGTAATT+AGG + chr8.1:45656983-45657002 None:intergenic 25.0%
! TAAACTAAATGGTGAAATTC+TGG + chr8.1:45657965-45657984 None:intergenic 25.0%
! TCAAATGGTCTATATTTGTA+GGG + chr8.1:45656894-45656913 None:intergenic 25.0%
! TTCAAATGGTCTATATTTGT+AGG + chr8.1:45656895-45656914 None:intergenic 25.0%
! TTTATACATGTAATTTGGGA+CGG + chr8.1:45656843-45656862 None:intergenic 25.0%
!! CATATTTTCATGGCTTAAAT+AGG + chr8.1:45657217-45657236 None:intergenic 25.0%
!! GACATATAATTTGGAATGAA+GGG - chr8.1:45657305-45657324 MS.gene34704:CDS 25.0%
!! TAAAGTGACTCATATTTTCA+TGG + chr8.1:45657227-45657246 None:intergenic 25.0%
!! TCACCATTTAGTTTATCTAA+AGG - chr8.1:45657970-45657989 MS.gene34704:intron 25.0%
!! TGACATATAATTTGGAATGA+AGG - chr8.1:45657304-45657323 MS.gene34704:CDS 25.0%
!!! CTAATTAGGTTTTTTTGTTG+AGG + chr8.1:45657094-45657113 None:intergenic 25.0%
!!! TAACCATTCACTTTGTTTTA+GGG - chr8.1:45656708-45656727 MS.gene34704:CDS 25.0%
!!! TACCAATATTAACTTGTTGT+TGG - chr8.1:45657895-45657914 MS.gene34704:intron 25.0%
AAATTCTGGCATATCTAGAA+TGG + chr8.1:45657951-45657970 None:intergenic 30.0%
AAGTTCCAAAGTTTCAAGAA+TGG + chr8.1:45657516-45657535 None:intergenic 30.0%
ACTTAGGACGAAAATTCAAA+TGG + chr8.1:45656909-45656928 None:intergenic 30.0%
AGCCAACAACAAGTTAATAT+TGG + chr8.1:45657900-45657919 None:intergenic 30.0%
AGTTCCAAAGTTTCAAGAAT+GGG + chr8.1:45657515-45657534 None:intergenic 30.0%
GTCTAGTTGAACTAAAACTT+TGG - chr8.1:45658086-45658105 MS.gene34704:CDS 30.0%
TAACCCTAAAACAAAGTGAA+TGG + chr8.1:45656714-45656733 None:intergenic 30.0%
TAATATTTGCAGTGGCAAAT+TGG + chr8.1:45656943-45656962 None:intergenic 30.0%
TACATCTGATTAGTAACTTC+AGG - chr8.1:45656986-45657005 MS.gene34704:CDS 30.0%
! AATTCTGGCATATCTAGAAT+GGG + chr8.1:45657950-45657969 None:intergenic 30.0%
! ATTGGAAGGATTAGTAACTT+AGG + chr8.1:45656925-45656944 None:intergenic 30.0%
!! AGAAAACTCAAGTTGAAAGA+AGG + chr8.1:45658004-45658023 None:intergenic 30.0%
!! TTTGACTTTTGGTCCTAATT+AGG + chr8.1:45657108-45657127 None:intergenic 30.0%
!!! ATTAGGTTTTTTTGTTGAGG+TGG + chr8.1:45657091-45657110 None:intergenic 30.0%
!!! GTAACCATTCACTTTGTTTT+AGG - chr8.1:45656707-45656726 MS.gene34704:CDS 30.0%
!!! TTAGGTTTTTTTGTTGAGGT+GGG + chr8.1:45657090-45657109 None:intergenic 30.0%
AAGTGTGATATGAGACAGTT+CGG + chr8.1:45658234-45658253 None:intergenic 35.0%
ACGATGAATTGGTCAATCTT+CGG - chr8.1:45657555-45657574 MS.gene34704:intron 35.0%
ACTAGGATACAACTATAGCT+TGG - chr8.1:45657454-45657473 MS.gene34704:intron 35.0%
CACTATCAGGATGAGAAAAT+AGG + chr8.1:45657748-45657767 None:intergenic 35.0%
GTTCCAAAGTTTCAAGAATG+GGG + chr8.1:45657514-45657533 None:intergenic 35.0%
TCTTTCGTTCCTGCAAATAA+AGG + chr8.1:45657194-45657213 None:intergenic 35.0%
TGAAAATCACTGTTGAGGTT+GGG - chr8.1:45657600-45657619 MS.gene34704:intron 35.0%
TTCAGGGACGAAAATTCAAA+TGG - chr8.1:45657168-45657187 MS.gene34704:CDS 35.0%
TTGAAAATCACTGTTGAGGT+TGG - chr8.1:45657599-45657618 MS.gene34704:intron 35.0%
TTTCTCAATGCTGCTACTAT+CGG - chr8.1:45657686-45657705 MS.gene34704:intron 35.0%
! ATTCTGGCATATCTAGAATG+GGG + chr8.1:45657949-45657968 None:intergenic 35.0%
! TTCAAATGGCCTTTATTTGC+AGG - chr8.1:45657182-45657201 MS.gene34704:CDS 35.0%
!! ACTTTGGAACTTCTTTCCTT+TGG - chr8.1:45657524-45657543 MS.gene34704:intron 35.0%
!! AGTGATTTTCAAGCTCTTCA+AGG + chr8.1:45657591-45657610 None:intergenic 35.0%
!!! CTGTGCCTGCATTTTTTATT+TGG - chr8.1:45658110-45658129 MS.gene34704:CDS 35.0%
AAGAGAAGAGAGTGATAGGA+AGG + chr8.1:45656665-45656684 None:intergenic 40.0%
AGACAGTTCGGAACATTCTT+AGG + chr8.1:45658222-45658241 None:intergenic 40.0%
AGAGAAGAGAGTGATAGGAA+GGG + chr8.1:45656664-45656683 None:intergenic 40.0%
ATGGGAAGGTGATGAATCAT+CGG + chr8.1:45658184-45658203 None:intergenic 40.0%
ATTTGCAGTGGCAAATTGGA+AGG + chr8.1:45656939-45656958 None:intergenic 40.0%
CGATGAATTGGTCAATCTTC+GGG - chr8.1:45657556-45657575 MS.gene34704:intron 40.0%
GAACTTCTTTCCTTTGGTCT+CGG - chr8.1:45657530-45657549 MS.gene34704:intron 40.0%
GAGCTTGAAAATCACTGTTG+AGG - chr8.1:45657595-45657614 MS.gene34704:intron 40.0%
GGTTCAACAGATTCACTATC+AGG + chr8.1:45657761-45657780 None:intergenic 40.0%
GTTATGAGAGTTTGTAGCAC+AGG + chr8.1:45658049-45658068 None:intergenic 40.0%
TAATATTTGCAGCGGCAGAT+CGG - chr8.1:45657132-45657151 MS.gene34704:CDS 40.0%
TAGCTTGGATATGAATTCCC+TGG - chr8.1:45657469-45657488 MS.gene34704:intron 40.0%
TCTCATAACATACGATGACC+AGG - chr8.1:45658060-45658079 MS.gene34704:CDS 40.0%
TTAGTTCAACTAGACACACC+TGG + chr8.1:45658081-45658100 None:intergenic 40.0%
TTCAAGCTCTTCAAGGAAGA+TGG + chr8.1:45657584-45657603 None:intergenic 40.0%
TTCGAATTGCAAGACACTTC+CGG - chr8.1:45657401-45657420 MS.gene34704:intron 40.0%
! AGCATTGAGAAATGTGACAG+CGG + chr8.1:45657678-45657697 None:intergenic 40.0%
! CGGAAGGATTAGTAACTTCA+GGG - chr8.1:45657152-45657171 MS.gene34704:CDS 40.0%
! TCGGAAGGATTAGTAACTTC+AGG - chr8.1:45657151-45657170 MS.gene34704:CDS 40.0%
! TTCTGGCATATCTAGAATGG+GGG + chr8.1:45657948-45657967 None:intergenic 40.0%
AGATGAAATTCCTGGAGGTG+GGG + chr8.1:45656618-45656637 None:intergenic 45.0%
AGCCAGAAAGAAGGATATCC+AGG + chr8.1:45657490-45657509 None:intergenic 45.0%
AGTTTCATCACCTTCAGCGA+TGG + chr8.1:45657437-45657456 None:intergenic 45.0%
ATCACTGTTGAGGTTGGGTA+TGG - chr8.1:45657605-45657624 MS.gene34704:intron 45.0%
ATGGTTACCTGAGAGAGAGT+AGG + chr8.1:45656695-45656714 None:intergenic 45.0%
ATTCATCACCTTCCCATGGA+TGG - chr8.1:45658187-45658206 MS.gene34704:CDS 45.0%
CCAATCATCACTTGCCACAT+GGG - chr8.1:45657044-45657063 MS.gene34704:CDS 45.0%
CCATGTGGCAAGTGATGATT+GGG + chr8.1:45657046-45657065 None:intergenic 45.0%
CTGCCCCATTCTTGAAACTT+TGG - chr8.1:45657508-45657527 MS.gene34704:intron 45.0%
GAATCATCGGTGACTGATGT+TGG + chr8.1:45658171-45658190 None:intergenic 45.0%
GAATTTCATCTCGAGCTCGA+GGG - chr8.1:45656627-45656646 MS.gene34704:intron 45.0%
GAGATGAAATTCCTGGAGGT+GGG + chr8.1:45656619-45656638 None:intergenic 45.0%
GAGCAAGAGAAGAGAGTGAT+AGG + chr8.1:45656669-45656688 None:intergenic 45.0%
GATCTGTTTACCATCGCTGA+AGG - chr8.1:45657424-45657443 MS.gene34704:intron 45.0%
GATGATTCATCACCTTCCCA+TGG - chr8.1:45658183-45658202 MS.gene34704:CDS 45.0%
GCCAGAAAGAAGGATATCCA+GGG + chr8.1:45657489-45657508 None:intergenic 45.0%
GCTACATAGCTCAACAACAC+AGG - chr8.1:45657826-45657845 MS.gene34704:intron 45.0%
GTAAGTTGTCCTTTGTTGCC+AGG - chr8.1:45658140-45658159 MS.gene34704:CDS 45.0%
GTAATGTACCTCAGTCTCGA+TGG - chr8.1:45657368-45657387 MS.gene34704:intron 45.0%
GTTTAAGTCCAGAGAGAGCA+CGG + chr8.1:45657799-45657818 None:intergenic 45.0%
TCACTGTTGAGGTTGGGTAT+GGG - chr8.1:45657606-45657625 MS.gene34704:intron 45.0%
TCCCTGGATATCCTTCTTTC+TGG - chr8.1:45657485-45657504 MS.gene34704:intron 45.0%
TCGAATTGCAAGACACTTCC+GGG - chr8.1:45657402-45657421 MS.gene34704:intron 45.0%
TCGCTGAAGGTGATGAAACT+AGG - chr8.1:45657437-45657456 MS.gene34704:intron 45.0%
TGACTGATGTTGGATCTTCC+TGG + chr8.1:45658161-45658180 None:intergenic 45.0%
TGGTCTCGGTTACGATGAAT+TGG - chr8.1:45657544-45657563 MS.gene34704:intron 45.0%
TTCATCACCTTCCCATGGAT+GGG - chr8.1:45658188-45658207 MS.gene34704:CDS 45.0%
TTCATCGTAACCGAGACCAA+AGG + chr8.1:45657543-45657562 None:intergenic 45.0%
TTGGATCTTCCTGGCAACAA+AGG + chr8.1:45658152-45658171 None:intergenic 45.0%
TTGGGACGGAGCAAGTAAAT+TGG + chr8.1:45656829-45656848 None:intergenic 45.0%
! TGTGTAGAACGCTTCAGGTT+CGG + chr8.1:45656559-45656578 None:intergenic 45.0%
CAGCGATGGTAAACAGATCC+CGG + chr8.1:45657423-45657442 None:intergenic 50.0%
CCCAATCATCACTTGCCACA+TGG - chr8.1:45657043-45657062 MS.gene34704:CDS 50.0%
CCCATGTGGCAAGTGATGAT+TGG + chr8.1:45657047-45657066 None:intergenic 50.0%
CGAGATGAAATTCCTGGAGG+TGG + chr8.1:45656620-45656639 None:intergenic 50.0%
CGAGCTCGAGATGAAATTCC+TGG + chr8.1:45656626-45656645 None:intergenic 50.0%
GAGTGTGTGTAGAACGCTTC+AGG + chr8.1:45656564-45656583 None:intergenic 50.0%
GCACGGAGGAGTTTGAGTAA+AGG + chr8.1:45657782-45657801 None:intergenic 50.0%
GCTCGAGATGAAATTCCTGG+AGG + chr8.1:45656623-45656642 None:intergenic 50.0%
GCTCTAACCTACTCTCTCTC+AGG - chr8.1:45656685-45656704 MS.gene34704:CDS 50.0%
GGAATTTCATCTCGAGCTCG+AGG - chr8.1:45656626-45656645 MS.gene34704:intron 50.0%
TCCTGATGTGACGTGAGCTA+CGG + chr8.1:45656512-45656531 None:intergenic 50.0%
! AGGCTAAGGTACTTGAGAGC+AGG + chr8.1:45657653-45657672 None:intergenic 50.0%
AAACTCCTCCGTGCTCTCTC+TGG - chr8.1:45657788-45657807 MS.gene34704:intron 55.0%
ATCACCTTCCCATGGATGGG+CGG - chr8.1:45658191-45658210 MS.gene34704:CDS 55.0%
ATTTGCAGCGGCAGATCGGA+AGG - chr8.1:45657136-45657155 MS.gene34704:CDS 55.0%
CAAGTGATGATTGGGCGAGG+TGG + chr8.1:45657038-45657057 None:intergenic 55.0%
CGATGCAACCATCGAGACTG+AGG + chr8.1:45657379-45657398 None:intergenic 55.0%
GAATGGGGCAGCCAGAAAGA+AGG + chr8.1:45657499-45657518 None:intergenic 55.0%
GAGCAGGCGCGTCTATTTCA+AGG + chr8.1:45657637-45657656 None:intergenic 55.0%
TCCGTAGCTCACGTCACATC+AGG - chr8.1:45656508-45656527 MS.gene34704:CDS 55.0%
TGAGAAATGTGACAGCGGCG+AGG + chr8.1:45657673-45657692 None:intergenic 55.0%
TGATGATTGGGCGAGGTGGT+TGG + chr8.1:45657034-45657053 None:intergenic 55.0%
TGGCAAGTGATGATTGGGCG+AGG + chr8.1:45657041-45657060 None:intergenic 55.0%
TTAGCAACGTGGTGCCCATG+TGG + chr8.1:45657061-45657080 None:intergenic 55.0%
! GTGGGCTGTCATTAGCAACG+TGG + chr8.1:45657072-45657091 None:intergenic 55.0%
! TAAGTCCAGAGAGAGCACGG+AGG + chr8.1:45657796-45657815 None:intergenic 55.0%
! TGTTGGCTGTGTGCAAGCAG+TGG - chr8.1:45657912-45657931 MS.gene34704:intron 55.0%
!! ACACACACTCAGTCGACTCG+TGG - chr8.1:45656574-45656593 MS.gene34704:intron 55.0%
!! GAGGACGGCGTTGACGAAAA+CGG + chr8.1:45656484-45656503 None:intergenic 55.0%
AGGCTTTGCCGCCCATCCAT+GGG + chr8.1:45658202-45658221 None:intergenic 60.0%
ATGTGACAGCGGCGAGGCTA+AGG + chr8.1:45657667-45657686 None:intergenic 60.0%
CGGCGAGAACTTGCACAACG+TGG - chr8.1:45657706-45657725 MS.gene34704:intron 60.0%
CGTGGATCCGTACAGCCGTT+GGG - chr8.1:45656592-45656611 MS.gene34704:intron 60.0%
TAGGCTTTGCCGCCCATCCA+TGG + chr8.1:45658203-45658222 None:intergenic 60.0%
TCGTGGATCCGTACAGCCGT+TGG - chr8.1:45656591-45656610 MS.gene34704:intron 60.0%
TGAGCTACGGAGAGCGAGGA+CGG + chr8.1:45656499-45656518 None:intergenic 60.0%
TTTGCCGCCCATCCATGGGA+AGG + chr8.1:45658198-45658217 None:intergenic 60.0%
GACGTGAGCTACGGAGAGCG+AGG + chr8.1:45656503-45656522 None:intergenic 65.0%
TTCCTGGAGGTGGGGCCCAA+CGG + chr8.1:45656610-45656629 None:intergenic 65.0%
GGTGGGGCCCAACGGCTGTA+CGG + chr8.1:45656602-45656621 None:intergenic 70.0%
AGCCGTTGGGCCCCACCTCC+AGG - chr8.1:45656605-45656624 MS.gene34704:intron 75.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 45656471 45658275 45656471 ID=MS.gene34704
chr8.1 mRNA 45656471 45658275 45656471 ID=MS.gene34704.t1;Parent=MS.gene34704
chr8.1 exon 45658040 45658275 45658040 ID=MS.gene34704.t1.exon1;Parent=MS.gene34704.t1
chr8.1 CDS 45658040 45658275 45658040 ID=cds.MS.gene34704.t1;Parent=MS.gene34704.t1
chr8.1 exon 45656899 45657364 45656899 ID=MS.gene34704.t1.exon2;Parent=MS.gene34704.t1
chr8.1 CDS 45656899 45657364 45656899 ID=cds.MS.gene34704.t1;Parent=MS.gene34704.t1
chr8.1 exon 45656665 45656814 45656665 ID=MS.gene34704.t1.exon3;Parent=MS.gene34704.t1
chr8.1 CDS 45656665 45656814 45656665 ID=cds.MS.gene34704.t1;Parent=MS.gene34704.t1
chr8.1 exon 45656471 45656584 45656471 ID=MS.gene34704.t1.exon4;Parent=MS.gene34704.t1
chr8.1 CDS 45656471 45656584 45656471 ID=cds.MS.gene34704.t1;Parent=MS.gene34704.t1
Gene Sequence

>MS.gene34704

ATGTCATTCACCGTTTTCGTCAACGCCGTCCTCGCTCTCCGTAGCTCACGTCACATCAGGAAGTTCAGTCTCATCTGCTATGACTCCGAACCTGAAGCGTTCTACACACACTCAGTCGACTCGTGGATCCGTACAGCCGTTGGGCCCCACCTCCAGGAATTTCATCTCGAGCTCGAGGGAGATGACTTCACCCTTCCTATCACTCTCTTCTCTTGCTCTAACCTACTCTCTCTCAGGTAACCATTCACTTTGTTTTAGGGTTAATTGCAGTTTCTACTAATTCTAAAATTGAATGCTCTTAAACCTTAATATTCCAAATATTGATATGACTTGTAATGATATGAGCTAAGCTGTTCCAATTTACTTGCTCCGTCCCAAATTACATGTATAAATGAAAATAGGCTTAAATAGGTCTTTCGTCCCTACAAATATAGACCATTTGAATTTTCGTCCTAAGTTACTAATCCTTCCAATTTGCCACTGCAAATATTAATCATTTGAAAAATGGTCCTAATTACATCTGATTAGTAACTTCAGGACGAAAATTCAAATGATTTAAGCCAACCACCTCGCCCAATCATCACTTGCCACATGGGCACCACGTTGCTAATGACAGCCCACCTCAACAAAAAAACCTAATTAGGACCAAAAGTCAAATGATTAATATTTGCAGCGGCAGATCGGAAGGATTAGTAACTTCAGGGACGAAAATTCAAATGGCCTTTATTTGCAGGAACGAAAGACCTATTTAAGCCATGAAAATATGAGTCACTTTACAAAAATATCTTTTGAGAAAAATAAATTAACATTAATGTTTCATTGTTATTGTAAAATGACATATAATTTGGAATGAAGGGAGTAATCTAAACGTGATTTTGATACTAACATTCATGTAATGTAATGTACCTCAGTCTCGATGGTTGCATCGAGTTCGAATTGCAAGACACTTCCGGGATCTGTTTACCATCGCTGAAGGTGATGAAACTAGGATACAACTATAGCTTGGATATGAATTCCCTGGATATCCTTCTTTCTGGCTGCCCCATTCTTGAAACTTTGGAACTTCTTTCCTTTGGTCTCGGTTACGATGAATTGGTCAATCTTCGGGTTCCATCTTCCTTGAAGAGCTTGAAAATCACTGTTGAGGTTGGGTATGGGATTTGCCTTGAAATAGACGCGCCTGCTCTCAAGTACCTTAGCCTCGCCGCTGTCACATTTCTCAATGCTGCTACTATCGGCGAGAACTTGCACAACGTGGAAGAAGCAGATCTTGACCTATTTTCTCATCCTGATAGTGAATCTGTTGAACCTTTACTCAAACTCCTCCGTGCTCTCTCTGGACTTAAACATCTAGAGCTACATAGCTCAACAACACAGGTGATTGAAGTTTTCATTATTTATGTTTCTGTTATATATGTACTCTCTACCAATATTAACTTGTTGTTGGCTGTGTGCAAGCAGTGGCTATTTTCGTCCCCCATTCTAGATATGCCAGAATTTCACCATTTAGTTTATCTAAAGGTCTCTTTTCCTTCTTTCAACTTGAGTTTTCTGTTCGATGTGCTTCAAAAGTGTCCTGTGCTACAAACTCTCATAACATACGATGACCAGGTGTGTCTAGTTGAACTAAAACTTTGGTCTGTGCCTGCATTTTTTATTTGGATCTTATGTAAGTTGTCCTTTGTTGCCAGGAAGATCCAACATCAGTCACCGATGATTCATCACCTTCCCATGGATGGGCGGCAAAGCCTAAGAATGTTCCGAACTGTCTCATATCACACTTGACCTTTATTTACTTCCAATGA
Protein sequence

>MS.gene34704.t1

MSFTVFVNAVLALRSSRHIRKFSLICYDSEPEAFYTHSVDSWIRTAVGPHLQEFHLELEGDDFTLPITLFSCSNLLSLSLDGCIEFELQDTSGICLPSLKVMKLGYNYSLDMNSLDILLSGCPILETLELLSFGLGYDELVNLRVPSSLKSLKITVEVGYGICLEIDAPALKYLSLAAVTFLNAATIGENLHNVEEADLDLFSHPDSESVEPLLKLLRALSGLKHLELHSSTTQWLFSSPILDMPEFHHLVYLKVSFPSFNLSFLFDVLQKCPVLQTLITYDDQEDPTSVTDDSSPSHGWAAKPKNVPNCLISHLTFIYFQ