AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25298

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25298.t1 MTR_8g062300 87.137 482 33 2 1 453 1 482 0.0 842
MS.gene25298.t1 MTR_8g070950 58.785 461 158 10 1 452 1 438 2.31e-164 471
MS.gene25298.t1 MTR_8g062010 59.080 435 161 9 26 452 9 434 2.62e-159 457
MS.gene25298.t1 MTR_8g062080 54.329 462 183 9 5 453 4 450 3.21e-158 455
MS.gene25298.t1 MTR_8g062310 55.606 437 175 8 16 452 15 432 2.33e-153 442
MS.gene25298.t1 MTR_8g058965 50.847 472 189 9 13 453 2 461 7.14e-144 419
MS.gene25298.t1 MTR_8g469020 50.980 459 183 10 26 452 7 455 8.22e-140 409
MS.gene25298.t1 MTR_4g050250 48.843 432 165 14 7 394 1 420 1.26e-112 338
MS.gene25298.t1 MTR_8g467790 48.187 386 145 8 25 393 147 494 6.26e-101 311
MS.gene25298.t1 MTR_2g083840 40.402 448 235 14 24 448 19 457 6.53e-81 258
MS.gene25298.t1 MTR_8g468810 38.994 477 248 15 1 448 1 463 1.31e-77 249
MS.gene25298.t1 MTR_8g058970 38.174 482 248 15 10 448 3 477 2.43e-77 249
MS.gene25298.t1 MTR_8g062270 39.520 458 227 18 23 447 13 453 2.67e-77 248
MS.gene25298.t1 MTR_8g014260 37.500 472 250 17 1 448 1 451 3.50e-72 235
MS.gene25298.t1 MTR_2g086020 79.577 142 29 0 311 452 178 319 8.79e-72 229
MS.gene25298.t1 MTR_2g086020 59.589 146 54 4 52 196 1 142 1.74e-41 150
MS.gene25298.t1 MTR_5g092960 35.532 470 260 11 9 447 4 461 1.72e-68 225
MS.gene25298.t1 MTR_8g467800 37.500 472 252 14 1 448 1 453 1.41e-67 223
MS.gene25298.t1 MTR_4g032730 34.728 478 256 12 13 447 2 466 9.25e-67 221
MS.gene25298.t1 MTR_8g468840 36.161 448 246 13 26 446 14 448 8.40e-64 213
MS.gene25298.t1 MTR_8g468760 36.203 453 217 15 25 447 8 418 2.60e-63 211
MS.gene25298.t1 MTR_8g445780 38.084 407 216 17 13 401 2 390 4.30e-56 191
MS.gene25298.t1 MTR_8g468810 38.903 383 191 15 5 358 3 371 1.22e-53 184
MS.gene25298.t1 MTR_5g092960 35.279 377 208 9 9 358 4 371 3.23e-47 167
MS.gene25298.t1 MTR_8g077460 32.517 449 208 18 19 448 3 375 2.82e-44 159
MS.gene25298.t1 MTR_8g468810 40.196 306 148 13 5 288 3 295 3.39e-44 157
MS.gene25298.t1 MTR_8g468810 40.196 306 148 13 5 288 3 295 6.04e-44 157
MS.gene25298.t1 MTR_8g468810 39.024 328 165 13 5 310 3 317 8.80e-44 157
MS.gene25298.t1 MTR_8g014160 31.306 444 225 14 26 448 41 425 2.02e-40 150
MS.gene25298.t1 MTR_8g467800 37.003 327 173 11 1 310 1 311 2.11e-34 132
MS.gene25298.t1 MTR_4g024710 34.007 297 174 11 167 448 58 347 5.73e-31 122
MS.gene25298.t1 MTR_3g099800 65.517 87 29 1 360 445 19 105 1.59e-29 111
MS.gene25298.t1 MTR_8g014210 41.398 186 104 5 264 448 50 231 4.11e-28 111
MS.gene25298.t1 MTR_1g041245 29.841 439 231 19 25 448 10 386 7.48e-25 105
MS.gene25298.t1 MTR_7g098510 25.917 436 256 11 22 448 14 391 1.59e-24 105
MS.gene25298.t1 MTR_3g107100 28.348 448 248 18 20 448 5 398 1.19e-23 102
MS.gene25298.t1 MTR_7g056433 28.341 434 244 17 26 448 12 389 1.43e-23 102
MS.gene25298.t1 MTR_5g084070 27.674 430 275 16 25 440 12 419 3.84e-23 101
MS.gene25298.t1 MTR_4g024745 32.746 284 165 11 165 431 10 284 1.40e-21 94.7
MS.gene25298.t1 MTR_1089s0010 25.734 443 248 19 21 448 3 379 7.18e-21 94.4
MS.gene25298.t1 MTR_3g108150 27.778 432 244 15 26 447 13 386 1.15e-20 94.0
MS.gene25298.t1 MTR_5g080710 28.049 410 263 14 25 419 32 424 1.30e-20 94.4
MS.gene25298.t1 MTR_2g007450 27.093 454 251 20 23 445 13 417 2.21e-20 93.2
MS.gene25298.t1 MTR_3g107160 26.634 413 224 16 21 415 5 356 1.82e-19 90.1
MS.gene25298.t1 MTR_5g021550 42.308 156 75 8 20 166 13 162 2.51e-19 85.1
MS.gene25298.t1 MTR_7g098480 27.064 436 247 14 22 447 14 388 6.73e-19 89.4
MS.gene25298.t1 MTR_1g085120 27.232 448 247 20 19 448 160 546 1.26e-18 89.0
MS.gene25298.t1 MTR_5g008950 24.654 434 245 16 28 448 1 365 1.54e-18 87.4
MS.gene25298.t1 MTR_1g085120 27.232 448 247 20 19 448 85 471 1.60e-18 88.2
MS.gene25298.t1 MTR_5g073420 25.666 413 215 16 25 419 10 348 2.41e-18 87.0
MS.gene25298.t1 MTR_5g045170 26.256 438 255 16 28 453 16 397 3.30e-18 86.7
MS.gene25298.t1 MTR_3g036250 27.107 439 221 21 26 445 5 363 6.64e-18 85.5
MS.gene25298.t1 MTR_3g107120 26.877 413 222 16 26 415 9 364 6.98e-18 85.5
MS.gene25298.t1 MTR_8g014290 38.217 157 76 8 26 167 10 160 2.28e-17 79.7
MS.gene25298.t1 MTR_2g036470 26.351 444 259 19 18 447 6 395 2.29e-17 84.0
MS.gene25298.t1 MTR_3g023630 27.397 438 237 21 23 448 5 373 1.15e-16 81.6
MS.gene25298.t1 MTR_7g056420 26.168 428 228 16 26 442 9 359 1.68e-16 82.8
MS.gene25298.t1 MTR_7g056447 31.707 205 121 8 26 224 9 200 1.81e-16 80.1
MS.gene25298.t1 MTR_7g056430 26.168 428 228 16 26 442 9 359 1.93e-16 82.8
MS.gene25298.t1 MTR_7g115270 26.037 434 261 15 26 445 12 399 3.29e-16 80.5
MS.gene25298.t1 MTR_7g055943 26.106 452 243 21 22 448 36 421 3.50e-16 80.9
MS.gene25298.t1 MTR_7g007080 27.021 433 243 18 26 445 4 376 6.27e-16 79.7
MS.gene25298.t1 MTR_5g007410 25.349 430 257 15 28 445 28 405 6.90e-16 80.1
MS.gene25298.t1 MTR_4g024720 40.141 142 65 8 1 136 1 128 7.32e-16 76.6
MS.gene25298.t1 MTR_2g007400 25.277 451 252 17 25 448 11 403 7.44e-16 79.7
MS.gene25298.t1 MTR_2g008320 25.287 435 256 14 25 448 51 427 7.51e-16 79.7
MS.gene25298.t1 MTR_6g022360 24.057 424 253 14 2 419 19 379 2.10e-15 78.2
MS.gene25298.t1 MTR_5g008920 25.901 444 240 21 23 448 9 381 3.13e-15 77.4
MS.gene25298.t1 MTR_7g026500 24.554 448 245 16 26 447 14 394 2.73e-14 74.7
MS.gene25298.t1 MTR_5g083890 24.942 429 253 16 26 430 6 389 3.36e-14 75.1
MS.gene25298.t1 MTR_6g022500 25.542 415 226 19 19 415 94 443 4.76e-14 74.3
MS.gene25298.t1 MTR_8g014230 41.667 84 47 2 358 441 17 98 6.67e-14 68.2
MS.gene25298.t1 MTR_6g011460 25.653 421 233 16 12 417 32 387 1.34e-13 72.8
MS.gene25298.t1 MTR_3g040700 24.938 401 219 15 26 414 43 373 2.11e-13 72.4
MS.gene25298.t1 MTR_7g032380 33.503 197 113 8 25 216 10 193 2.74e-13 71.2
MS.gene25298.t1 MTR_5g058000 25.517 435 245 18 25 447 7 374 4.85e-13 70.9
MS.gene25298.t1 MTR_1g107385 26.305 479 248 19 11 445 3 420 6.36e-13 70.9
MS.gene25298.t1 MTR_3g467400 29.808 208 121 9 26 217 3 201 2.25e-12 68.6
MS.gene25298.t1 MTR_8g085430 30.049 203 119 7 25 216 10 200 2.90e-12 68.2
MS.gene25298.t1 MTR_4g098590 26.588 425 228 21 28 427 1 366 1.58e-11 66.2
MS.gene25298.t1 MTR_8g468910 30.808 198 107 8 7 191 29 209 2.09e-11 65.9
MS.gene25298.t1 MTR_2g007500 30.732 205 107 8 21 216 9 187 2.52e-11 65.1
MS.gene25298.t1 MTR_1g492880 40.698 86 49 2 26 110 6 90 3.15e-11 60.1
MS.gene25298.t1 MTR_7g032370 32.847 137 84 3 319 448 217 352 3.94e-11 64.7
MS.gene25298.t1 MTR_4g113810 35.537 121 67 5 107 221 3 118 4.22e-11 62.0
MS.gene25298.t1 MTR_1g492880 38.889 90 53 2 22 110 2 90 4.61e-11 60.1
MS.gene25298.t1 MTR_4g024770 48.611 72 27 2 1 72 8 69 7.20e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25298.t1 AT5G56420 25.835 449 275 14 26 448 6 422 8.34e-20 91.7
MS.gene25298.t1 AT5G56420 25.835 449 275 14 26 448 6 422 8.34e-20 91.7
MS.gene25298.t1 AT5G56420 25.835 449 275 14 26 448 6 422 8.34e-20 91.7
MS.gene25298.t1 AT1G78750 25.157 477 289 20 12 445 4 455 1.81e-19 90.9
MS.gene25298.t1 AT1G78750 25.157 477 289 20 12 445 4 455 1.81e-19 90.9
MS.gene25298.t1 AT5G56560 27.982 436 275 20 28 448 7 418 1.12e-16 82.4
MS.gene25298.t1 AT4G26340 27.027 444 279 16 25 448 1 419 1.42e-16 82.0
MS.gene25298.t1 AT1G69630 24.845 483 280 17 10 445 2 448 1.65e-16 82.0
MS.gene25298.t1 AT3G50710 25.918 463 268 18 25 448 1 427 3.07e-16 80.9
MS.gene25298.t1 AT5G56440 25.597 461 273 15 25 447 1 429 1.09e-15 79.3
MS.gene25298.t1 AT1G66290 25.311 482 286 28 2 445 5 450 3.55e-15 77.8
MS.gene25298.t1 AT3G42770 26.364 440 274 13 28 440 1 417 7.70e-15 77.0
MS.gene25298.t1 AT5G53840 24.736 473 274 19 15 445 5 437 1.24e-14 76.3
MS.gene25298.t1 AT5G60610 24.213 413 264 15 25 415 1 386 3.01e-14 74.7
MS.gene25298.t1 AT5G60610 24.213 413 264 15 25 415 1 386 3.01e-14 74.7
MS.gene25298.t1 AT5G44940 23.853 436 260 14 24 448 3 377 3.88e-14 74.3
MS.gene25298.t1 AT3G59160 28.889 225 120 9 19 214 8 221 4.18e-14 74.7
MS.gene25298.t1 AT5G56810 26.667 465 281 18 7 445 1 431 6.61e-14 73.9
MS.gene25298.t1 AT5G50270 25.117 426 262 12 25 420 1 399 4.12e-13 71.2
MS.gene25298.t1 AT1G16930 31.818 198 111 10 26 216 15 195 6.25e-13 70.9
MS.gene25298.t1 AT1G16930 31.818 198 111 10 26 216 15 195 6.25e-13 70.9
MS.gene25298.t1 AT1G16930 31.818 198 111 10 26 216 15 195 6.25e-13 70.9
MS.gene25298.t1 AT1G13780 24.569 464 285 18 26 445 10 452 1.11e-12 70.1
MS.gene25298.t1 AT1G78760 23.789 475 287 21 19 449 2 445 2.51e-12 68.9
MS.gene25298.t1 AT3G59190 30.288 208 120 10 19 214 7 201 3.14e-12 68.6
MS.gene25298.t1 AT3G59190 30.348 201 117 9 26 214 12 201 3.17e-12 68.9
MS.gene25298.t1 AT5G22720 23.973 438 257 18 26 415 23 432 3.33e-12 68.6
MS.gene25298.t1 AT3G59170 30.455 220 113 9 26 214 7 217 8.33e-12 67.4
MS.gene25298.t1 AT4G14103 28.571 196 122 6 26 214 8 192 1.19e-11 67.0
MS.gene25298.t1 AT2G42720 30.256 195 126 5 25 214 1 190 1.33e-11 66.6
MS.gene25298.t1 AT1G66310 24.895 474 281 22 10 445 3 439 1.46e-11 66.6
MS.gene25298.t1 AT3G59200 28.141 199 121 6 26 214 7 193 1.61e-11 66.2
MS.gene25298.t1 AT4G14103 28.571 196 122 6 26 214 8 192 1.77e-11 65.9
MS.gene25298.t1 AT4G14103 28.571 196 122 6 26 214 8 192 1.77e-11 65.9
MS.gene25298.t1 AT3G59200 28.141 199 121 6 26 214 7 193 2.57e-11 66.2
MS.gene25298.t1 AT3G26922 27.574 272 171 7 17 285 5 253 3.85e-11 64.3
MS.gene25298.t1 AT3G26922 27.574 272 171 7 17 285 5 253 3.85e-11 64.3
MS.gene25298.t1 AT1G55030 25.054 459 291 19 10 447 5 431 5.38e-11 64.7

Find 115 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAAACACTGCAGTCTTTC+TGG 0.129894 8.2:-38292061 MS.gene25298:intron
AAACGAAGGATATATTAATT+TGG 0.153932 8.2:-38291792 MS.gene25298:CDS
CCCCAAGCTGGAAACTTTGC+AGG 0.229332 8.2:-38291924 MS.gene25298:CDS
GATTGGCCGGTTTCGAGCTC+TGG 0.257085 8.2:+38291843 None:intergenic
GTCTCTCGGCACAGAAATTA+TGG 0.288190 8.2:-38291981 MS.gene25298:CDS
ACCTGCAAAGTTTCCAGCTT+GGG 0.309530 8.2:+38291923 None:intergenic
AACGAAGGATATATTAATTT+GGG 0.327225 8.2:-38291791 MS.gene25298:CDS
CTCGCCGGTGGCGCCACCTC+TGG 0.341008 8.2:-38292746 MS.gene25298:CDS
CTGACCTATGTTCACATAGA+AGG 0.351633 8.2:-38291186 MS.gene25298:CDS
TATATTAATTTGGGCATTGT+AGG 0.357147 8.2:-38291782 MS.gene25298:CDS
ATGTTTCAACTCAAATCTTC+TGG 0.358687 8.2:-38291461 MS.gene25298:CDS
GAGATAGAGGTGTTGAAGTT+GGG 0.360637 8.2:+38292509 None:intergenic
ACTAGGAGGTGGTCGAGAAC+AGG 0.375934 8.2:+38291692 None:intergenic
AGAGATAGAGGTGTTGAAGT+TGG 0.394213 8.2:+38292508 None:intergenic
GTAGACGGTAACAGTAGAAT+TGG 0.397098 8.2:+38292706 None:intergenic
TTCCAACTAGAACCTCTGTT+TGG 0.407347 8.2:-38292785 MS.gene25298:CDS
TTGGAAGGAAGGACATGATA+TGG 0.420389 8.2:+38292802 None:intergenic
TGTAGGCAACTTTCACACTA+TGG 0.425217 8.2:-38291765 MS.gene25298:CDS
AAATTACTTTCCTTCCTCTC+TGG 0.429603 8.2:-38291950 MS.gene25298:CDS
CCTCTCTGGCTGCCCCAAGC+TGG 0.444251 8.2:-38291936 MS.gene25298:CDS
GCTCCAACCTCCTTTCCCTT+AGG 0.450370 8.2:-38292434 MS.gene25298:intron
TCTTTCTGGTAAGATCAGTA+TGG 0.453817 8.2:-38292047 MS.gene25298:CDS
AATTCAGGATAATTTAGAAC+AGG 0.454737 8.2:+38291517 None:intergenic
CCTTCCAGAGGTGGCGCCAC+CGG 0.463852 8.2:+38292742 None:intergenic
CCGTAGATTTATAGCATCAT+GGG 0.467450 8.2:+38291667 None:intergenic
TGCGGAAATTGCGGAGGTTG+AGG 0.468343 8.2:+38292640 None:intergenic
CGGCCAATCATAATTTCACT+TGG 0.470608 8.2:-38291829 MS.gene25298:CDS
CAATTGATGAAGAGGGAAGG+AGG 0.470818 8.2:+38292456 None:intergenic
AGTCCAAGTGAAATTATGAT+TGG 0.480791 8.2:+38291826 None:intergenic
TTATAGCATCATGGGGAACT+AGG 0.481247 8.2:+38291675 None:intergenic
AATTGATGAAGAGGGAAGGA+GGG 0.486231 8.2:+38292457 None:intergenic
AACCTGCAAAGTTTCCAGCT+TGG 0.491460 8.2:+38291922 None:intergenic
GACCCAACTGTATCCTCATA+AGG 0.492983 8.2:-38290988 None:intergenic
ATTGGCCGGTTTCGAGCTCT+GGG 0.493701 8.2:+38291844 None:intergenic
ACCGTAGATTTATAGCATCA+TGG 0.495398 8.2:+38291666 None:intergenic
AACAGTAGAATTGGAAGACT+TGG 0.497863 8.2:+38292715 None:intergenic
CAATGTGAACATAGAGATAG+AGG 0.499821 8.2:+38292496 None:intergenic
TTCCTCCCAGAGCTCGAAAC+CGG 0.515568 8.2:-38291849 MS.gene25298:CDS
AACTCTTCTGCTGATTTCTG+TGG 0.519465 8.2:+38292888 None:intergenic
ATATGGCACAGGAGTGTGTT+CGG 0.519601 8.2:+38292819 None:intergenic
AAGATTAACACATATATCAA+AGG 0.520576 8.2:-38291037 MS.gene25298:CDS
GTCCAAACAGAGGTTCTAGT+TGG 0.524498 8.2:+38292783 None:intergenic
TACCTTATGAGGATACAGTT+GGG 0.528201 8.2:+38290986 None:intergenic
TGAATGGAATTGAATGTAGA+CGG 0.528341 8.2:+38292691 None:intergenic
CTTGGGGCAGCCAGAGAGGA+AGG 0.529957 8.2:+38291940 None:intergenic
GGTTGGAGCAATTGATGAAG+AGG 0.530052 8.2:+38292448 None:intergenic
TTCGAGCTCTGGGAGGAAGA+TGG 0.530767 8.2:+38291854 None:intergenic
GTTCACATAGAAGGGTATCA+AGG 0.534998 8.2:-38291177 MS.gene25298:CDS
CGACAAATTCACTCTCAAAA+CGG 0.535261 8.2:+38291717 None:intergenic
CAAGTGAAATTATGATTGGC+CGG 0.542813 8.2:+38291830 None:intergenic
CTTCTATGTGAACATAGGTC+AGG 0.542971 8.2:+38291187 None:intergenic
TCCGGTGTCCATGTTCTCAA+TGG 0.544353 8.2:+38291243 None:intergenic
CCCATGATGCTATAAATCTA+CGG 0.544523 8.2:-38291667 MS.gene25298:CDS
TGATGAAGAGGGAAGGAGGG+AGG 0.545199 8.2:+38292460 None:intergenic
CTATGAGCCTCGTCTCTCGC+CGG 0.546013 8.2:-38292761 MS.gene25298:CDS
AACCACCACCATTGAGAACA+TGG 0.546378 8.2:-38291251 MS.gene25298:CDS
CCGGTGGCGCCACCTCTGGA+AGG 0.547084 8.2:-38292742 MS.gene25298:CDS
CAGTTGGGTCGAACTTAAGT+TGG 0.547514 8.2:+38291001 None:intergenic
TTACCTAAGGGAAAGGAGGT+TGG 0.550204 8.2:+38292431 None:intergenic
GTGTTGAAGTTGGGGTCCAA+TGG 0.551379 8.2:+38292518 None:intergenic
GCATTGGTCACAAAGACTGC+AGG 0.551894 8.2:+38291884 None:intergenic
CCAGCTTGGGGCAGCCAGAG+AGG 0.557168 8.2:+38291936 None:intergenic
TTACCTTATGAGGATACAGT+TGG 0.557869 8.2:+38290985 None:intergenic
AGATTAACACATATATCAAA+GGG 0.561216 8.2:-38291036 MS.gene25298:CDS
GTTGGAGCAATTGATGAAGA+GGG 0.561648 8.2:+38292449 None:intergenic
GGAAGATGGAACGAGAGCAT+TGG 0.564694 8.2:+38291868 None:intergenic
GTTGGGGTCCAATGGCATCA+AGG 0.569817 8.2:+38292526 None:intergenic
TCGTGGATCCTTGATGCCAT+TGG 0.570896 8.2:-38292534 MS.gene25298:CDS
TCAGAAACTGTTGTTGACTC+CGG 0.578291 8.2:+38291225 None:intergenic
TATGAATGTTGCGGAAATTG+CGG 0.578436 8.2:+38292631 None:intergenic
ATACCCTTCTATGTGAACAT+AGG 0.580469 8.2:+38291182 None:intergenic
AGATAGAGGTGTTGAAGTTG+GGG 0.580903 8.2:+38292510 None:intergenic
AAACAGAGGTTCTAGTTGGA+AGG 0.581002 8.2:+38292787 None:intergenic
GTCCATGTTCTCAATGGTGG+TGG 0.584631 8.2:+38291249 None:intergenic
GAGCAATTGATGAAGAGGGA+AGG 0.585056 8.2:+38292453 None:intergenic
AGAAGATTTGAGTTGAAACA+TGG 0.586977 8.2:+38291463 None:intergenic
CCTGCAAAGTTTCCAGCTTG+GGG 0.588595 8.2:+38291924 None:intergenic
CTATGTGAACATAGGTCAGG+TGG 0.588925 8.2:+38291190 None:intergenic
TGACCTATGTTCACATAGAA+GGG 0.590318 8.2:-38291185 MS.gene25298:CDS
AGAGACAACATCTTCAGTGA+TGG 0.590452 8.2:+38291998 None:intergenic
GGAAGGACATGATATGGCAC+AGG 0.590863 8.2:+38292808 None:intergenic
AGAGGTTCTAGTTGGAAGGA+AGG 0.597523 8.2:+38292791 None:intergenic
CTAGGAGGTGGTCGAGAACA+GGG 0.599798 8.2:+38291693 None:intergenic
TGAGAGGTTACCTAAGGGAA+AGG 0.607320 8.2:+38292424 None:intergenic
CATCATGGGGAACTAGGAGG+TGG 0.608053 8.2:+38291681 None:intergenic
TCACTGAAGATGTTGTCTCT+CGG 0.611749 8.2:-38291995 MS.gene25298:CDS
GGCGCCACCGGCGAGAGACG+AGG 0.612718 8.2:+38292754 None:intergenic
GATGGCAGAATCAAGAAGCA+TGG 0.615963 8.2:-38292913 MS.gene25298:CDS
CTTGGAGATCCTTCCAGAGG+TGG 0.619949 8.2:+38292733 None:intergenic
TGAGCCTCGTCTCTCGCCGG+TGG 0.621828 8.2:-38292758 MS.gene25298:CDS
TTGTCCACTGTGTTGAATCG+TGG 0.624204 8.2:-38292551 MS.gene25298:CDS
TTCTGGTAAGATCAGTATGG+AGG 0.627628 8.2:-38292044 MS.gene25298:CDS
GATGGAACTTATGAATGTTG+CGG 0.629837 8.2:+38292622 None:intergenic
GTTGCGTCATTCGACATCAA+AGG 0.630198 8.2:-38291645 MS.gene25298:intron
GAGTTAGTAAACGATGGCGG+AGG 0.630834 8.2:-38292870 MS.gene25298:CDS
GATGAAAGGTGAGATCGAGA+TGG 0.636927 8.2:+38292604 None:intergenic
TATGTGAACATAGGTCAGGT+GGG 0.643030 8.2:+38291191 None:intergenic
GGTGTCCATGTTCTCAATGG+TGG 0.646260 8.2:+38291246 None:intergenic
ACCATTGAGAACATGGACAC+CGG 0.646446 8.2:-38291244 MS.gene25298:CDS
GGCCGGTTTCGAGCTCTGGG+AGG 0.664051 8.2:+38291847 None:intergenic
ACTTACTTCGATATAAACGA+AGG 0.665297 8.2:-38291806 MS.gene25298:CDS
TTATGAGAACTTGAAGCATG+CGG 0.670506 8.2:+38291419 None:intergenic
AGGCAACTTTCACACTATGG+AGG 0.670571 8.2:-38291762 MS.gene25298:CDS
GAGGTTACCTAAGGGAAAGG+AGG 0.670966 8.2:+38292427 None:intergenic
TGCGGCATTTCTCAAGCACG+TGG 0.671395 8.2:+38291437 None:intergenic
CGTAGATTTATAGCATCATG+GGG 0.673823 8.2:+38291668 None:intergenic
GCAGAAGAGTTAGTAAACGA+TGG 0.674951 8.2:-38292876 MS.gene25298:CDS
TCTCATAATTCAGAGCAACA+AGG 0.677248 8.2:-38291404 MS.gene25298:intron
TAGCATCATGGGGAACTAGG+AGG 0.681420 8.2:+38291678 None:intergenic
AGACTTGGAGATCCTTCCAG+AGG 0.695151 8.2:+38292730 None:intergenic
GAAGAGTTAGTAAACGATGG+CGG 0.698804 8.2:-38292873 MS.gene25298:CDS
GAATGTTGCGGAAATTGCGG+AGG 0.710599 8.2:+38292634 None:intergenic
TTTAATGGACAAAACAGACA+AGG 0.711577 8.2:-38291073 MS.gene25298:CDS
GAGGCTCATAGTCCAAACAG+AGG 0.753115 8.2:+38292773 None:intergenic
GGATCCACGATTCAACACAG+TGG 0.768479 8.2:+38292547 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATATTAGTAATTTGA+TGG + chr8.2:38291738-38291757 None:intergenic 10.0%
!! CATAATATATAGTAATAAGA+AGG + chr8.2:38291526-38291545 None:intergenic 15.0%
!!! TATTTTTGTTGATAGTTTAA+TGG - chr8.2:38292814-38292833 MS.gene25298:CDS 15.0%
!! AAACGAAGGATATATTAATT+TGG - chr8.2:38292110-38292129 MS.gene25298:intron 20.0%
!! AACGAAGGATATATTAATTT+GGG - chr8.2:38292111-38292130 MS.gene25298:intron 20.0%
!! AAGATTAACACATATATCAA+AGG - chr8.2:38292865-38292884 MS.gene25298:CDS 20.0%
!! AGATTAACACATATATCAAA+GGG - chr8.2:38292866-38292885 MS.gene25298:CDS 20.0%
!! GTCATTACTCAAAAATATAT+TGG + chr8.2:38292604-38292623 None:intergenic 20.0%
!! TGAGCATAAAAATAAATGAT+TGG + chr8.2:38291596-38291615 None:intergenic 20.0%
!!! AATAACAGAAACTTTTTGAA+TGG + chr8.2:38291230-38291249 None:intergenic 20.0%
!!! TATATTTTTGAGTAATGACA+TGG - chr8.2:38292605-38292624 MS.gene25298:CDS 20.0%
!!! TTTTATGCTCAAATTGAAAA+GGG - chr8.2:38291605-38291624 MS.gene25298:intron 20.0%
!!! TTTTTATGCTCAAATTGAAA+AGG - chr8.2:38291604-38291623 MS.gene25298:intron 20.0%
! GAAATAAGAGTTGTCAAAAA+CGG + chr8.2:38291327-38291346 None:intergenic 25.0%
!! AATTCAGGATAATTTAGAAC+AGG + chr8.2:38292388-38292407 None:intergenic 25.0%
!! TATATTAATTTGGGCATTGT+AGG - chr8.2:38292120-38292139 MS.gene25298:intron 25.0%
AAAATTGATACTGCAATGAG+AGG + chr8.2:38291497-38291516 None:intergenic 30.0%
ACATGCATGTATATATTGAC+AGG + chr8.2:38292562-38292581 None:intergenic 30.0%
ACTTACTTCGATATAAACGA+AGG - chr8.2:38292096-38292115 MS.gene25298:intron 30.0%
AGTCCAAGTGAAATTATGAT+TGG + chr8.2:38292079-38292098 None:intergenic 30.0%
ATAGCAATGTTGTGTCAATT+AGG - chr8.2:38291637-38291656 MS.gene25298:intron 30.0%
ATGTTTCAACTCAAATCTTC+TGG - chr8.2:38292441-38292460 MS.gene25298:CDS 30.0%
TGAATGGAATTGAATGTAGA+CGG + chr8.2:38291214-38291233 None:intergenic 30.0%
! AAGGTGAGGTTATACTTTTA+TGG - chr8.2:38292276-38292295 MS.gene25298:intron 30.0%
! GCTGAATATATTTTGCAGAA+CGG - chr8.2:38292770-38292789 MS.gene25298:CDS 30.0%
! TATATTGACCTGTCAGTTTT+TGG - chr8.2:38291820-38291839 MS.gene25298:CDS 30.0%
! TTAGTAATTTGATGGCAAAC+AGG + chr8.2:38291730-38291749 None:intergenic 30.0%
! TTTAATGGACAAAACAGACA+AGG - chr8.2:38292829-38292848 MS.gene25298:CDS 30.0%
! TTTTGTAAATCGCGAAATTC+AGG + chr8.2:38292403-38292422 None:intergenic 30.0%
!! AGAAGATTTGAGTTGAAACA+TGG + chr8.2:38292442-38292461 None:intergenic 30.0%
!!! ATTGGTTTTGTCATCCAAAA+AGG - chr8.2:38291684-38291703 MS.gene25298:CDS 30.0%
AAATTACTTTCCTTCCTCTC+TGG - chr8.2:38291952-38291971 MS.gene25298:CDS 35.0%
AACAGTAGAATTGGAAGACT+TGG + chr8.2:38291190-38291209 None:intergenic 35.0%
ACCGTAGATTTATAGCATCA+TGG + chr8.2:38292239-38292258 None:intergenic 35.0%
AGTGTTTACCAAAAACTGAC+AGG + chr8.2:38291831-38291850 None:intergenic 35.0%
ATACCCTTCTATGTGAACAT+AGG + chr8.2:38292723-38292742 None:intergenic 35.0%
CAAGTGAAATTATGATTGGC+CGG + chr8.2:38292075-38292094 None:intergenic 35.0%
CAATGTGAACATAGAGATAG+AGG + chr8.2:38291409-38291428 None:intergenic 35.0%
CCCATGATGCTATAAATCTA+CGG - chr8.2:38292235-38292254 MS.gene25298:intron 35.0%
CCGTAGATTTATAGCATCAT+GGG + chr8.2:38292238-38292257 None:intergenic 35.0%
CGACAAATTCACTCTCAAAA+CGG + chr8.2:38292188-38292207 None:intergenic 35.0%
CGTAGATTTATAGCATCATG+GGG + chr8.2:38292237-38292256 None:intergenic 35.0%
CTTTCGATGGTCTATTTAAG+AGG - chr8.2:38291661-38291680 MS.gene25298:CDS 35.0%
GATGGAACTTATGAATGTTG+CGG + chr8.2:38291283-38291302 None:intergenic 35.0%
GATGGTCTATTTAAGAGGAT+TGG - chr8.2:38291666-38291685 MS.gene25298:CDS 35.0%
TATGAATGTTGCGGAAATTG+CGG + chr8.2:38291274-38291293 None:intergenic 35.0%
TCTCATAATTCAGAGCAACA+AGG - chr8.2:38292498-38292517 MS.gene25298:CDS 35.0%
TGACCTATGTTCACATAGAA+GGG - chr8.2:38292717-38292736 MS.gene25298:CDS 35.0%
TGTCATCCAAAAAGGATTAG+CGG - chr8.2:38291692-38291711 MS.gene25298:CDS 35.0%
TTATGAGAACTTGAAGCATG+CGG + chr8.2:38292486-38292505 None:intergenic 35.0%
! TCTTTCTGGTAAGATCAGTA+TGG - chr8.2:38291855-38291874 MS.gene25298:CDS 35.0%
AATTGATGAAGAGGGAAGGA+GGG + chr8.2:38291448-38291467 None:intergenic 40.0%
AGAGACAACATCTTCAGTGA+TGG + chr8.2:38291907-38291926 None:intergenic 40.0%
CGGCCAATCATAATTTCACT+TGG - chr8.2:38292073-38292092 MS.gene25298:intron 40.0%
CTGACCTATGTTCACATAGA+AGG - chr8.2:38292716-38292735 MS.gene25298:CDS 40.0%
CTTCTATGTGAACATAGGTC+AGG + chr8.2:38292718-38292737 None:intergenic 40.0%
GAAGAGTTAGTAAACGATGG+CGG - chr8.2:38291029-38291048 MS.gene25298:CDS 40.0%
GACATGGTTTCCTTTATTGC+TGG - chr8.2:38292621-38292640 MS.gene25298:CDS 40.0%
GCAGAAGAGTTAGTAAACGA+TGG - chr8.2:38291026-38291045 MS.gene25298:CDS 40.0%
GTAGACGGTAACAGTAGAAT+TGG + chr8.2:38291199-38291218 None:intergenic 40.0%
GTCAAAAACGGAACGATGAA+AGG + chr8.2:38291315-38291334 None:intergenic 40.0%
GTGTCAATTAGGTCTTTCGA+TGG - chr8.2:38291648-38291667 MS.gene25298:CDS 40.0%
GTTGGAGCAATTGATGAAGA+GGG + chr8.2:38291456-38291475 None:intergenic 40.0%
TATGTGAACATAGGTCAGGT+GGG + chr8.2:38292714-38292733 None:intergenic 40.0%
TCACTGAAGATGTTGTCTCT+CGG - chr8.2:38291907-38291926 MS.gene25298:CDS 40.0%
TGCAATGAGAGGTTACCTAA+GGG + chr8.2:38291486-38291505 None:intergenic 40.0%
TGGTTTCCTTTATTGCTGGT+AGG - chr8.2:38292625-38292644 MS.gene25298:CDS 40.0%
TGTAGGCAACTTTCACACTA+TGG - chr8.2:38292137-38292156 MS.gene25298:intron 40.0%
TTATAGCATCATGGGGAACT+AGG + chr8.2:38292230-38292249 None:intergenic 40.0%
TTCCAACTAGAACCTCTGTT+TGG - chr8.2:38291117-38291136 MS.gene25298:CDS 40.0%
TTCTGGTAAGATCAGTATGG+AGG - chr8.2:38291858-38291877 MS.gene25298:CDS 40.0%
TTGACAGGTCAGAGATTGTT+TGG + chr8.2:38292547-38292566 None:intergenic 40.0%
TTGGAAGGAAGGACATGATA+TGG + chr8.2:38291103-38291122 None:intergenic 40.0%
TTTCCTTTATTGCTGGTAGG+AGG - chr8.2:38292628-38292647 MS.gene25298:CDS 40.0%
! AAACAGAGGTTCTAGTTGGA+AGG + chr8.2:38291118-38291137 None:intergenic 40.0%
!! AACTCTTCTGCTGATTTCTG+TGG + chr8.2:38291017-38291036 None:intergenic 40.0%
!! AGAGATAGAGGTGTTGAAGT+TGG + chr8.2:38291397-38291416 None:intergenic 40.0%
!! AGATAGAGGTGTTGAAGTTG+GGG + chr8.2:38291395-38291414 None:intergenic 40.0%
!! GAGATAGAGGTGTTGAAGTT+GGG + chr8.2:38291396-38291415 None:intergenic 40.0%
!! GTTCACATAGAAGGGTATCA+AGG - chr8.2:38292725-38292744 MS.gene25298:CDS 40.0%
!! TCAGAAACTGTTGTTGACTC+CGG + chr8.2:38292680-38292699 None:intergenic 40.0%
AACCACCACCATTGAGAACA+TGG - chr8.2:38292651-38292670 MS.gene25298:CDS 45.0%
AACCTGCAAAGTTTCCAGCT+TGG + chr8.2:38291983-38292002 None:intergenic 45.0%
ACCATTGAGAACATGGACAC+CGG - chr8.2:38292658-38292677 MS.gene25298:CDS 45.0%
ACCTGCAAAGTTTCCAGCTT+GGG + chr8.2:38291982-38292001 None:intergenic 45.0%
AGGCAACTTTCACACTATGG+AGG - chr8.2:38292140-38292159 MS.gene25298:intron 45.0%
CAATTGATGAAGAGGGAAGG+AGG + chr8.2:38291449-38291468 None:intergenic 45.0%
CAGTTGGGTCGAACTTAAGT+TGG + chr8.2:38292904-38292923 None:intergenic 45.0%
CTATGTGAACATAGGTCAGG+TGG + chr8.2:38292715-38292734 None:intergenic 45.0%
CTGCAATGAGAGGTTACCTA+AGG + chr8.2:38291487-38291506 None:intergenic 45.0%
GAGCAATTGATGAAGAGGGA+AGG + chr8.2:38291452-38291471 None:intergenic 45.0%
GATGAAAGGTGAGATCGAGA+TGG + chr8.2:38291301-38291320 None:intergenic 45.0%
GGTAAACACTGCAGTCTTTC+TGG - chr8.2:38291841-38291860 MS.gene25298:CDS 45.0%
GGTTGGAGCAATTGATGAAG+AGG + chr8.2:38291457-38291476 None:intergenic 45.0%
GTCATTCGACATCAAAGGTG+AGG - chr8.2:38292262-38292281 MS.gene25298:intron 45.0%
GTCTCTCGGCACAGAAATTA+TGG - chr8.2:38291921-38291940 MS.gene25298:CDS 45.0%
GTTCCTCCTACCAGCAATAA+AGG + chr8.2:38292634-38292653 None:intergenic 45.0%
GTTGCGTCATTCGACATCAA+AGG - chr8.2:38292257-38292276 MS.gene25298:intron 45.0%
TGAGAGGTTACCTAAGGGAA+AGG + chr8.2:38291481-38291500 None:intergenic 45.0%
TTACCTAAGGGAAAGGAGGT+TGG + chr8.2:38291474-38291493 None:intergenic 45.0%
TTGTCCACTGTGTTGAATCG+TGG - chr8.2:38291351-38291370 MS.gene25298:intron 45.0%
! AGAGGTTCTAGTTGGAAGGA+AGG + chr8.2:38291114-38291133 None:intergenic 45.0%
! ATATGGCACAGGAGTGTGTT+CGG + chr8.2:38291086-38291105 None:intergenic 45.0%
! GTCCAAACAGAGGTTCTAGT+TGG + chr8.2:38291122-38291141 None:intergenic 45.0%
!!! TGCAGAACGGACTTGTTTTG+AGG - chr8.2:38292783-38292802 MS.gene25298:CDS 45.0%
AGACTTGGAGATCCTTCCAG+AGG + chr8.2:38291175-38291194 None:intergenic 50.0%
CCTGCAAAGTTTCCAGCTTG+GGG + chr8.2:38291981-38292000 None:intergenic 50.0%
GAATGTTGCGGAAATTGCGG+AGG + chr8.2:38291271-38291290 None:intergenic 50.0%
GAGGCTCATAGTCCAAACAG+AGG + chr8.2:38291132-38291151 None:intergenic 50.0%
GAGGTTACCTAAGGGAAAGG+AGG + chr8.2:38291478-38291497 None:intergenic 50.0%
GGAAGGACATGATATGGCAC+AGG + chr8.2:38291097-38291116 None:intergenic 50.0%
GGATCCACGATTCAACACAG+TGG + chr8.2:38291358-38291377 None:intergenic 50.0%
GGTGTCCATGTTCTCAATGG+TGG + chr8.2:38292659-38292678 None:intergenic 50.0%
GTCCATGTTCTCAATGGTGG+TGG + chr8.2:38292656-38292675 None:intergenic 50.0%
GTGTTGAAGTTGGGGTCCAA+TGG + chr8.2:38291387-38291406 None:intergenic 50.0%
TAGCATCATGGGGAACTAGG+AGG + chr8.2:38292227-38292246 None:intergenic 50.0%
TCCGGTGTCCATGTTCTCAA+TGG + chr8.2:38292662-38292681 None:intergenic 50.0%
TCGTGGATCCTTGATGCCAT+TGG - chr8.2:38291368-38291387 MS.gene25298:intron 50.0%
! GAGTTAGTAAACGATGGCGG+AGG - chr8.2:38291032-38291051 MS.gene25298:CDS 50.0%
! GCATTGGTCACAAAGACTGC+AGG + chr8.2:38292021-38292040 None:intergenic 50.0%
! GGAAGATGGAACGAGAGCAT+TGG + chr8.2:38292037-38292056 None:intergenic 50.0%
ACTAGGAGGTGGTCGAGAAC+AGG + chr8.2:38292213-38292232 None:intergenic 55.0%
CATCATGGGGAACTAGGAGG+TGG + chr8.2:38292224-38292243 None:intergenic 55.0%
CCCCAAGCTGGAAACTTTGC+AGG - chr8.2:38291978-38291997 MS.gene25298:CDS 55.0%
CTAGGAGGTGGTCGAGAACA+GGG + chr8.2:38292212-38292231 None:intergenic 55.0%
CTTGGAGATCCTTCCAGAGG+TGG + chr8.2:38291172-38291191 None:intergenic 55.0%
GCTCCAACCTCCTTTCCCTT+AGG - chr8.2:38291468-38291487 MS.gene25298:CDS 55.0%
GTTGGGGTCCAATGGCATCA+AGG + chr8.2:38291379-38291398 None:intergenic 55.0%
TGATGAAGAGGGAAGGAGGG+AGG + chr8.2:38291445-38291464 None:intergenic 55.0%
TGCGGAAATTGCGGAGGTTG+AGG + chr8.2:38291265-38291284 None:intergenic 55.0%
TGCGGCATTTCTCAAGCACG+TGG + chr8.2:38292468-38292487 None:intergenic 55.0%
TTCCTCCCAGAGCTCGAAAC+CGG - chr8.2:38292053-38292072 MS.gene25298:intron 55.0%
! ATTGGCCGGTTTCGAGCTCT+GGG + chr8.2:38292061-38292080 None:intergenic 55.0%
! GCTGCGCCGCTAATCCTTTT+TGG + chr8.2:38291701-38291720 None:intergenic 55.0%
!! TTCGAGCTCTGGGAGGAAGA+TGG + chr8.2:38292051-38292070 None:intergenic 55.0%
CTATGAGCCTCGTCTCTCGC+CGG - chr8.2:38291141-38291160 MS.gene25298:CDS 60.0%
! GATTGGCCGGTTTCGAGCTC+TGG + chr8.2:38292062-38292081 None:intergenic 60.0%
CTTGGGGCAGCCAGAGAGGA+AGG + chr8.2:38291965-38291984 None:intergenic 65.0%
CCAGCTTGGGGCAGCCAGAG+AGG + chr8.2:38291969-38291988 None:intergenic 70.0%
CCTCTCTGGCTGCCCCAAGC+TGG - chr8.2:38291966-38291985 MS.gene25298:CDS 70.0%
CCTTCCAGAGGTGGCGCCAC+CGG + chr8.2:38291163-38291182 None:intergenic 70.0%
TGAGCCTCGTCTCTCGCCGG+TGG - chr8.2:38291144-38291163 MS.gene25298:CDS 70.0%
!! GGCCGGTTTCGAGCTCTGGG+AGG + chr8.2:38292058-38292077 None:intergenic 70.0%
CCGGTGGCGCCACCTCTGGA+AGG - chr8.2:38291160-38291179 MS.gene25298:CDS 75.0%
! CTCGCCGGTGGCGCCACCTC+TGG - chr8.2:38291156-38291175 MS.gene25298:CDS 80.0%
! GGCGCCACCGGCGAGAGACG+AGG + chr8.2:38291151-38291170 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 38290990 38292934 38290990 ID=MS.gene25298
chr8.2 mRNA 38290990 38292934 38290990 ID=MS.gene25298.t1;Parent=MS.gene25298
chr8.2 exon 38292435 38292934 38292435 ID=MS.gene25298.t1.exon1;Parent=MS.gene25298.t1
chr8.2 CDS 38292435 38292934 38292435 ID=cds.MS.gene25298.t1;Parent=MS.gene25298.t1
chr8.2 exon 38291646 38292069 38291646 ID=MS.gene25298.t1.exon2;Parent=MS.gene25298.t1
chr8.2 CDS 38291646 38292069 38291646 ID=cds.MS.gene25298.t1;Parent=MS.gene25298.t1
chr8.2 exon 38291405 38291554 38291405 ID=MS.gene25298.t1.exon3;Parent=MS.gene25298.t1
chr8.2 CDS 38291405 38291554 38291405 ID=cds.MS.gene25298.t1;Parent=MS.gene25298.t1
chr8.2 exon 38290990 38291277 38290990 ID=MS.gene25298.t1.exon4;Parent=MS.gene25298.t1
chr8.2 CDS 38290990 38291277 38290990 ID=cds.MS.gene25298.t1;Parent=MS.gene25298.t1
Gene Sequence

>MS.gene25298

ATGGCAGAATCAAGAAGCATGGAACCACAGAAATCAGCAGAAGAGTTAGTAAACGATGGCGGAGGCAATGACATCGACATATTGAGCAGTCTACCGAACACACTCCTGTGCCATATCATGTCCTTCCTTCCAACTAGAACCTCTGTTTGGACTATGAGCCTCGTCTCTCGCCGGTGGCGCCACCTCTGGAAGGATCTCCAAGTCTTCCAATTCTACTGTTACCGTCTACATTCAATTCCATTCAAAAAGTTTCTGTTATTCGTCAACACTGTCCTCAACCTCCGCAATTTCCGCAACATTCATAAGTTCCATCTCGATCTCACCTTTCATCGTTCCGTTTTTGACAACTCTTATTTCAACTTTGTCCACTGTGTTGAATCGTGGATCCTTGATGCCATTGGACCCCAACTTCAACACCTCTATCTCTATGTTCACATTGATGCTGTTATACACCTCCCTCCTTCCCTCTTCATCAATTGCTCCAACCTCCTTTCCCTTAGGTAACCTCTCATTGCAGTATCAATTTTGTTACACCTTCTTATTACTATATATTATGCACAAAATAATGCTTCACTAAGTTGTAATTACTAAACTCAATAATATCCAATCATTTATTTTTATGCTCAAATTGAAAAGGGTCATGATGAATAGCAATGTTGTGTCAATTAGGTCTTTCGATGGTCTATTTAAGAGGATTGGTTTTGTCATCCAAAAAGGATTAGCGGCGCAGCTTGTTGCCTGTTTGCCATCAAATTACTAATATTATTTTGTTTCATCTATTGTTCTGTTACTTTTTTTTTGTTTTGTTTTTTATAACAAATATAGAATGTTATATTGACCTGTCAGTTTTTGGTAAACACTGCAGTCTTTCTGGTAAGATCAGTATGGAGGTTAAACATTCTTCACTTCATTTTCCATCACTGAAGATGTTGTCTCTCGGCACAGAAATTATGGATGAAAGTAAATTACTTTCCTTCCTCTCTGGCTGCCCCAAGCTGGAAACTTTGCAGGTTTACTTTTACTTTCAACCTGCAGTCTTTGTGACCAATGCTCTCGTTCCATCTTCCTCCCAGAGCTCGAAACCGGCCAATCATAATTTCACTTGGACTTACTTCGATATAAACGAAGGATATATTAATTTGGGCATTGTAGGCAACTTTCACACTATGGAGGAAGCATTTCTCAATGTCTTTTACCGTTTTGAGAGTGAATTTGTCGACCCTGTTCTCGACCACCTCCTAGTTCCCCATGATGCTATAAATCTACGGTTGCGTCATTCGACATCAAAGGTGAGGTTATACTTTTATGGTTTCTAACTTTTTATACGTGTAGTTTCGTACTAATATTCTGCAATCACTTGTTGTTATCGTCTACAAGCAGTGTTCACTTCATGCTCCTGTTCTAAATTATCCTGAATTTCGCGATTTACAAAATCTGAAGTTCATTCTTCCATGTTTCAACTCAAATCTTCTGGTCCACGTGCTTGAGAAATGCCGCATGCTTCAAGTTCTCATAATTCAGAGCAACAAGGTATGTTAACTTCTCAATTTTAATCCAAACAATCTCTGACCTGTCAATATATACATGCATGTATGTATCTATCTGTACATACCAATATATTTTTGAGTAATGACATGGTTTCCTTTATTGCTGGTAGGAGGAACCACCACCATTGAGAACATGGACACCGGAGTCAACAACAGTTTCTGAATGTCTCAAATCCCACCTGACCTATGTTCACATAGAAGGGTATCAAGGACTTGAAGATGAGCTGACATTTGCTGAATATATTTTGCAGAACGGACTTGTTTTGAGGACAATGTTTATTTTTGTTGATAGTTTAATGGACAAAACAGACAAGGAATGTTCTCTCAAAAGATTAACACATATATCAAAGGGCTCTGTCACGTGCCAACTTAAGTTCGACCCAACTGTATCCTCATAA
Protein sequence

>MS.gene25298.t1

MAESRSMEPQKSAEELVNDGGGNDIDILSSLPNTLLCHIMSFLPTRTSVWTMSLVSRRWRHLWKDLQVFQFYCYRLHSIPFKKFLLFVNTVLNLRNFRNIHKFHLDLTFHRSVFDNSYFNFVHCVESWILDAIGPQLQHLYLYVHIDAVIHLPPSLFINCSNLLSLSLSGKISMEVKHSSLHFPSLKMLSLGTEIMDESKLLSFLSGCPKLETLQVYFYFQPAVFVTNALVPSSSQSSKPANHNFTWTYFDINEGYINLGIVGNFHTMEEAFLNVFYRFESEFVDPVLDHLLVPHDAINLRLRHSTSKCSLHAPVLNYPEFRDLQNLKFILPCFNSNLLVHVLEKCRMLQVLIIQSNKEEPPPLRTWTPESTTVSECLKSHLTYVHIEGYQGLEDELTFAEYILQNGLVLRTMFIFVDSLMDKTDKECSLKRLTHISKGSVTCQLKFDPTVSS