AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99980.t1 MTR_8g062310 75.216 347 78 3 1 344 15 356 0.0 510
MS.gene99980.t1 MTR_8g070950 55.882 340 119 10 11 329 15 344 5.58e-108 323
MS.gene99980.t1 MTR_8g062010 53.731 335 136 7 11 329 9 340 1.63e-101 306
MS.gene99980.t1 MTR_8g062080 50.141 355 145 11 1 333 15 359 2.04e-96 293
MS.gene99980.t1 MTR_8g469020 45.767 378 156 10 11 344 7 379 2.36e-95 291
MS.gene99980.t1 MTR_4g050250 48.533 375 141 11 2 329 14 383 2.35e-93 285
MS.gene99980.t1 MTR_8g058965 48.303 383 150 13 5 344 7 384 7.24e-92 282
MS.gene99980.t1 MTR_8g062300 46.015 389 159 11 1 340 16 402 1.64e-91 282
MS.gene99980.t1 MTR_8g467790 46.705 349 121 11 10 329 147 459 3.31e-77 246
MS.gene99980.t1 MTR_8g062270 39.714 350 169 11 10 324 15 357 5.20e-60 199
MS.gene99980.t1 MTR_4g032730 41.176 357 163 11 5 325 7 352 1.86e-58 196
MS.gene99980.t1 MTR_2g083840 40.058 347 181 8 9 329 19 364 1.17e-57 193
MS.gene99980.t1 MTR_8g468810 39.058 361 176 15 5 329 19 371 1.51e-55 186
MS.gene99980.t1 MTR_8g468810 38.830 376 185 16 5 344 19 385 2.18e-55 187
MS.gene99980.t1 MTR_8g467800 39.941 338 176 8 11 324 20 354 9.17e-54 183
MS.gene99980.t1 MTR_8g468810 40.625 288 135 11 5 263 19 299 5.29e-49 167
MS.gene99980.t1 MTR_8g468810 40.625 288 135 11 5 263 19 299 7.59e-49 167
MS.gene99980.t1 MTR_8g468810 40.625 288 135 11 5 263 19 299 1.41e-48 167
MS.gene99980.t1 MTR_8g058970 37.701 374 179 14 10 332 18 388 2.65e-47 166
MS.gene99980.t1 MTR_8g468840 39.326 356 179 12 11 334 14 364 5.64e-47 165
MS.gene99980.t1 MTR_8g445780 37.391 345 182 14 11 329 13 349 2.29e-46 162
MS.gene99980.t1 MTR_5g092960 37.853 354 176 12 10 324 18 366 4.45e-46 161
MS.gene99980.t1 MTR_5g092960 37.363 364 184 12 10 334 18 376 6.56e-46 162
MS.gene99980.t1 MTR_8g468760 38.746 351 150 11 1 321 1 316 2.36e-45 160
MS.gene99980.t1 MTR_8g014260 35.695 367 200 13 11 344 16 379 2.31e-43 155
MS.gene99980.t1 MTR_8g467800 40.433 277 138 8 11 263 20 293 2.01e-41 148
MS.gene99980.t1 MTR_2g086020 35.690 297 115 5 37 329 1 225 5.33e-38 138
MS.gene99980.t1 MTR_8g077460 34.473 351 151 16 1 332 1 291 1.01e-30 120
MS.gene99980.t1 MTR_5g021550 50.000 146 59 5 11 144 19 162 1.04e-29 112
MS.gene99980.t1 MTR_8g014160 37.313 268 138 8 8 247 38 303 2.52e-29 117
MS.gene99980.t1 MTR_8g014290 45.098 153 64 6 11 145 10 160 2.09e-27 105
MS.gene99980.t1 MTR_5g008950 35.135 185 107 7 13 193 1 176 2.16e-20 91.3
MS.gene99980.t1 MTR_7g056447 38.251 183 103 6 11 189 9 185 6.10e-20 89.0
MS.gene99980.t1 MTR_7g032380 36.946 203 106 8 1 192 1 192 1.60e-19 88.6
MS.gene99980.t1 MTR_1g041245 33.498 203 113 8 1 192 1 192 9.92e-19 86.7
MS.gene99980.t1 MTR_7g115270 37.755 196 102 8 11 192 12 201 1.50e-18 86.3
MS.gene99980.t1 MTR_8g014220 36.667 180 71 7 11 186 19 159 3.45e-18 80.9
MS.gene99980.t1 MTR_4g024720 39.024 164 66 7 11 144 17 176 1.96e-17 80.1
MS.gene99980.t1 MTR_2g007500 33.005 203 99 8 4 192 7 186 3.99e-17 81.6
MS.gene99980.t1 MTR_4g056020 32.258 217 119 7 11 211 9 213 7.02e-17 81.6
MS.gene99980.t1 MTR_5g073420 32.900 231 130 11 1 217 1 220 8.32e-17 81.3
MS.gene99980.t1 MTR_5g008920 35.233 193 116 6 3 192 4 190 1.51e-16 80.5
MS.gene99980.t1 MTR_6g011460 32.258 217 128 9 5 211 40 247 1.91e-16 80.5
MS.gene99980.t1 MTR_7g056433 35.602 191 109 8 11 193 12 196 3.31e-16 79.3
MS.gene99980.t1 MTR_8g085430 33.962 212 107 9 1 192 1 199 4.14e-16 78.6
MS.gene99980.t1 MTR_3g105060 27.632 304 182 13 10 286 13 305 1.04e-15 77.8
MS.gene99980.t1 MTR_3g108150 35.204 196 110 7 11 200 13 197 1.04e-15 77.8
MS.gene99980.t1 MTR_4g056030 31.455 213 126 7 11 211 9 213 9.48e-15 75.5
MS.gene99980.t1 MTR_7g034110 27.711 332 191 12 1 313 1 302 1.18e-14 74.3
MS.gene99980.t1 MTR_4g024710 33.163 196 109 8 160 335 73 266 1.38e-14 74.3
MS.gene99980.t1 MTR_3g023630 33.679 193 110 9 8 191 5 188 6.80e-14 72.4
MS.gene99980.t1 MTR_7g033990 30.864 243 140 10 1 223 1 235 7.34e-14 72.4
MS.gene99980.t1 MTR_4g056040 29.268 246 118 7 11 211 9 243 7.62e-14 72.8
MS.gene99980.t1 MTR_3g107100 31.606 193 115 9 11 194 11 195 9.14e-14 72.0
MS.gene99980.t1 MTR_5g083890 30.049 203 120 5 11 192 6 207 9.45e-14 72.4
MS.gene99980.t1 MTR_3g108560 33.333 186 108 6 11 192 20 193 1.08e-13 71.2
MS.gene99980.t1 MTR_8g014210 37.086 151 78 5 207 343 9 156 1.38e-13 69.7
MS.gene99980.t1 MTR_3g108560 33.333 186 108 6 11 192 20 193 1.63e-13 71.2
MS.gene99980.t1 MTR_2g007450 32.558 215 121 9 4 196 9 221 1.68e-13 71.6
MS.gene99980.t1 MTR_7g034115 31.980 197 108 8 10 193 10 193 2.87e-13 70.5
MS.gene99980.t1 MTR_5g084070 26.426 333 220 8 9 325 11 334 4.80e-13 70.1
MS.gene99980.t1 MTR_1g108820 32.447 188 111 7 10 191 7 184 5.21e-13 69.7
MS.gene99980.t1 MTR_1g108820 31.915 188 112 7 10 191 7 184 7.55e-13 69.3
MS.gene99980.t1 MTR_7g056420 31.522 184 92 8 11 191 9 161 7.91e-13 70.1
MS.gene99980.t1 MTR_5g058000 32.323 198 109 9 6 191 3 187 8.05e-13 69.3
MS.gene99980.t1 MTR_7g056430 31.522 184 92 8 11 191 9 161 9.32e-13 69.7
MS.gene99980.t1 MTR_1g085120 33.500 200 113 10 2 190 169 359 9.36e-13 69.3
MS.gene99980.t1 MTR_1g038710 34.021 194 110 8 11 191 19 207 9.78e-13 69.3
MS.gene99980.t1 MTR_1g085120 33.500 200 113 10 2 190 157 347 1.45e-12 68.9
MS.gene99980.t1 MTR_7g114710 31.250 192 114 6 10 192 12 194 1.47e-12 68.2
MS.gene99980.t1 MTR_1g085120 33.500 200 113 10 2 190 82 272 1.61e-12 68.6
MS.gene99980.t1 MTR_3g107160 28.870 239 144 10 3 224 2 231 3.59e-12 67.4
MS.gene99980.t1 MTR_7g098510 30.928 194 121 7 5 192 12 198 3.89e-12 67.0
MS.gene99980.t1 MTR_5g061680 33.990 203 118 10 1 194 1 196 4.39e-12 66.6
MS.gene99980.t1 MTR_7g026500 31.343 201 106 7 11 191 14 202 5.82e-12 66.6
MS.gene99980.t1 MTR_5g080710 25.740 338 222 11 9 325 31 360 6.67e-12 66.6
MS.gene99980.t1 MTR_8g066650 34.545 165 95 6 11 170 80 236 7.13e-12 66.6
MS.gene99980.t1 MTR_2g007400 30.769 208 111 11 10 193 11 209 8.22e-12 66.2
MS.gene99980.t1 MTR_7g033915 29.237 236 131 8 1 220 1 216 9.18e-12 65.9
MS.gene99980.t1 MTR_7g084080 30.876 217 114 8 4 212 10 198 9.97e-12 65.1
MS.gene99980.t1 MTR_3g099830 25.871 201 124 5 5 198 6 188 1.08e-11 65.9
MS.gene99980.t1 MTR_6g044560 29.289 239 133 10 1 207 168 402 1.19e-11 66.2
MS.gene99980.t1 MTR_6g044572 30.435 230 124 10 10 207 1 226 1.43e-11 65.5
MS.gene99980.t1 MTR_2g100590 30.396 227 131 10 10 217 41 259 1.79e-11 65.5
MS.gene99980.t1 MTR_2g100590 30.396 227 131 10 10 217 41 259 1.83e-11 65.5
MS.gene99980.t1 MTR_2g100590 30.396 227 131 10 10 217 41 259 1.89e-11 65.1
MS.gene99980.t1 MTR_6g025270 27.854 219 118 7 5 212 28 217 2.53e-11 64.7
MS.gene99980.t1 MTR_7g055943 32.020 203 103 11 7 191 36 221 2.81e-11 64.7
MS.gene99980.t1 MTR_4g024770 60.417 48 18 1 11 58 24 70 3.40e-11 58.9
MS.gene99980.t1 MTR_7g032370 34.158 202 104 10 23 207 2 191 4.58e-11 63.5
MS.gene99980.t1 MTR_4g098590 29.897 194 120 7 13 192 1 192 5.61e-11 63.5
MS.gene99980.t1 MTR_1089s0010 30.052 193 119 8 10 194 7 191 6.44e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99980.t1 AT1G16930 35.602 191 105 8 11 193 15 195 3.31e-18 85.9
MS.gene99980.t1 AT1G16930 35.602 191 105 8 11 193 15 195 3.31e-18 85.9
MS.gene99980.t1 AT1G16930 35.602 191 105 8 11 193 15 195 3.31e-18 85.9
MS.gene99980.t1 AT3G59200 30.075 266 141 12 11 242 7 261 4.38e-17 81.6
MS.gene99980.t1 AT3G59200 30.075 266 141 12 11 242 7 261 9.44e-17 81.6
MS.gene99980.t1 AT4G10400 34.896 192 109 8 10 193 1 184 2.80e-16 79.7
MS.gene99980.t1 AT4G10400 34.896 192 109 8 10 193 1 184 2.80e-16 79.7
MS.gene99980.t1 AT4G10400 34.896 192 109 8 10 193 1 184 2.80e-16 79.7
MS.gene99980.t1 AT1G69630 31.472 197 121 7 4 193 11 200 5.34e-16 79.3
MS.gene99980.t1 AT1G78750 28.365 208 127 8 6 197 13 214 1.66e-15 77.8
MS.gene99980.t1 AT1G78750 28.365 208 127 8 6 197 13 214 1.66e-15 77.8
MS.gene99980.t1 AT4G09920 34.737 190 108 8 10 191 1 182 1.66e-15 76.6
MS.gene99980.t1 AT4G09920 34.737 190 108 8 10 191 1 182 1.66e-15 76.6
MS.gene99980.t1 AT4G09920 34.737 190 108 8 10 191 1 182 1.86e-15 76.3
MS.gene99980.t1 AT4G09920 34.737 190 108 8 10 191 1 182 1.86e-15 76.3
MS.gene99980.t1 AT4G09920 34.737 190 108 8 10 191 1 182 1.86e-15 76.3
MS.gene99980.t1 AT3G60040 29.911 224 131 7 11 211 232 452 1.96e-15 77.8
MS.gene99980.t1 AT3G60040 29.911 224 131 7 11 211 230 450 2.03e-15 77.8
MS.gene99980.t1 AT3G60040 29.911 224 131 7 11 211 232 452 2.23e-15 77.8
MS.gene99980.t1 AT3G60040 29.911 224 131 7 11 211 13 233 2.25e-15 77.8
MS.gene99980.t1 AT3G60040 29.911 224 131 7 11 211 233 453 2.25e-15 77.8
MS.gene99980.t1 AT1G78760 27.948 229 142 10 11 226 16 234 2.08e-14 74.3
MS.gene99980.t1 AT5G22670 30.303 198 124 6 1 193 1 189 9.21e-14 72.4
MS.gene99980.t1 AT5G22700 26.554 354 216 13 4 343 39 362 1.38e-13 71.6
MS.gene99980.t1 AT3G59190 30.653 199 111 9 11 191 12 201 3.04e-13 70.5
MS.gene99980.t1 AT3G59190 30.653 199 111 9 11 191 12 201 3.82e-13 70.9
MS.gene99980.t1 AT4G14096 29.259 270 142 15 11 246 8 262 6.14e-13 70.1
MS.gene99980.t1 AT4G14096 29.259 270 142 15 11 246 8 262 6.14e-13 70.1
MS.gene99980.t1 AT2G26860 32.828 198 111 7 10 194 1 189 9.89e-13 68.9
MS.gene99980.t1 AT2G26860 32.828 198 111 7 10 194 1 189 9.89e-13 68.9
MS.gene99980.t1 AT5G38590 27.007 274 166 9 10 258 1 265 2.08e-12 67.4
MS.gene99980.t1 AT5G22700 31.019 216 124 9 4 210 39 238 2.75e-12 67.8
MS.gene99980.t1 AT5G22700 31.100 209 122 8 11 210 6 201 3.32e-12 67.8
MS.gene99980.t1 AT5G22700 31.100 209 122 8 11 210 6 201 3.32e-12 67.8
MS.gene99980.t1 AT5G22700 31.100 209 122 8 11 210 6 201 3.32e-12 67.8
MS.gene99980.t1 AT5G22700 31.100 209 122 8 11 210 6 201 3.32e-12 67.8
MS.gene99980.t1 AT5G38590 27.083 288 174 10 10 270 1 279 3.95e-12 67.4
MS.gene99980.t1 AT4G13960 32.512 203 111 8 10 197 1 192 6.33e-12 67.0
MS.gene99980.t1 AT4G13960 32.512 203 111 8 10 197 1 192 6.33e-12 67.0
MS.gene99980.t1 AT1G66290 25.357 280 176 12 2 257 19 289 8.74e-12 66.2
MS.gene99980.t1 AT2G04230 28.794 257 152 11 11 248 13 257 2.66e-11 64.7
MS.gene99980.t1 AT3G26922 27.341 267 159 11 5 256 7 253 5.23e-11 63.2
MS.gene99980.t1 AT3G26922 27.341 267 159 11 5 256 7 253 5.23e-11 63.2
MS.gene99980.t1 AT3G49150 31.818 220 124 11 11 213 16 226 5.72e-11 63.9
MS.gene99980.t1 AT4G13965 27.859 341 206 15 6 323 5 328 5.89e-11 63.9
MS.gene99980.t1 AT3G59170 29.268 246 121 15 11 213 7 242 6.50e-11 63.9

Find 81 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATGAGCCGTTTGTCTTCT+AGG 0.107723 8.4:-39276541 MS.gene99980:CDS
CAGATTTATCATGCCAATTT+AGG 0.160904 8.4:+39275022 None:intergenic
CACCACATCCGCTCCTAAAT+TGG 0.227291 8.4:-39275035 MS.gene99980:CDS
CTGCCCCAAGCTCGAAGATT+TGG 0.293891 8.4:-39275962 MS.gene99980:CDS
TTTGAAGGTGATTGAGAATA+GGG 0.309567 8.4:+39275746 None:intergenic
TGTTCTAAATAGCAAAATTC+TGG 0.312962 8.4:+39275560 None:intergenic
CAGAATATTTGAGTTAAAAC+AGG 0.317327 8.4:+39275520 None:intergenic
GGCTACACATTGGGCATTGC+AGG 0.327474 8.4:-39275835 MS.gene99980:CDS
AATTCTGGATAATTTATATC+AGG 0.327966 8.4:+39275575 None:intergenic
GTGTTCTGCTTCCACGATCT+TGG 0.332447 8.4:-39276491 MS.gene99980:CDS
CATCTTGGTGGCTACACATT+GGG 0.346345 8.4:-39275844 MS.gene99980:CDS
CATCAAATTCATAGATGAAA+TGG 0.346428 8.4:+39276390 None:intergenic
CCACCTCTGGAAAGATCTTC+AGG 0.348838 8.4:-39276513 MS.gene99980:CDS
AACAAATTCACTTTGACAAT+AGG 0.350640 8.4:+39275771 None:intergenic
ATCTCCAAATCTTCGAGCTT+GGG 0.358832 8.4:+39275958 None:intergenic
TGGAAACTTTGCAGTCTATC+TGG 0.377861 8.4:-39276093 MS.gene99980:intron
ATACATGCAGTGGCCACTTC+AGG 0.378400 8.4:-39275600 MS.gene99980:intron
ATCTGGTGGTAAATACAATA+TGG 0.383544 8.4:-39276076 MS.gene99980:CDS
ATCTTCGAGCTTGGGGCAGC+CGG 0.383876 8.4:+39275966 None:intergenic
TGCAGGCAACTTTCACACTA+TGG 0.398879 8.4:-39275818 MS.gene99980:CDS
CTTCTAGGTTGCGCCACCTC+TGG 0.403731 8.4:-39276526 MS.gene99980:CDS
GCTTGGACTTACCTTCATCT+TGG 0.407476 8.4:-39275859 MS.gene99980:CDS
CTTGGGGCAGCCGGAGAGAA+AGG 0.421545 8.4:+39275975 None:intergenic
TTGCAACAGACGTTCTTGTT+GGG 0.455034 8.4:+39276564 None:intergenic
TCAATAGTTGCCTTTCTCTC+CGG 0.462166 8.4:-39275985 MS.gene99980:CDS
AATCTCCAAATCTTCGAGCT+TGG 0.468488 8.4:+39275957 None:intergenic
GAGATCAAGTTCTTCAAGGC+GGG 0.483714 8.4:+39276307 None:intergenic
TGGACTTACCTTCATCTTGG+TGG 0.487659 8.4:-39275856 MS.gene99980:CDS
GTATTTCAACCTCTTGAAGC+TGG 0.488329 8.4:+39275897 None:intergenic
GTTGCAACAGACGTTCTTGT+TGG 0.488706 8.4:+39276563 None:intergenic
TTGTGTGGATGGAATCAGTT+TGG 0.490640 8.4:-39275932 MS.gene99980:CDS
ATGTGGGTCCGTTCTGCCAT+TGG 0.498043 8.4:-39276332 MS.gene99980:CDS
GAATGTGGCGCGATCTGCGG+AGG 0.501492 8.4:+39276420 None:intergenic
ACACCAATGATAATTTCGCT+TGG 0.513716 8.4:-39275876 MS.gene99980:CDS
TTGGAGATTTATTGTGTGGA+TGG 0.515453 8.4:-39275943 MS.gene99980:CDS
TGATGAGATCAAGTTCTTCA+AGG 0.519787 8.4:+39276303 None:intergenic
GCAATTTCCATCACTGAAGA+AGG 0.523230 8.4:-39276034 MS.gene99980:CDS
TGCCAATTTAGGAGCGGATG+TGG 0.524115 8.4:+39275033 None:intergenic
AGTCCAAGCGAAATTATCAT+TGG 0.524718 8.4:+39275873 None:intergenic
GACTACTTATTCTGTCGATG+TGG 0.529175 8.4:+39276624 None:intergenic
GCATGCATGGCGACTACGAA+TGG 0.531190 8.4:-39276653 None:intergenic
AGACGTTCTTGTTGGGAGGA+AGG 0.532307 8.4:+39276571 None:intergenic
GCAACAACCTCGTTTCCCTC+AGG 0.532637 8.4:-39276235 MS.gene99980:intron
GAAAGAACAAAAGATGAAGC+TGG 0.532643 8.4:+39275061 None:intergenic
TCATCTTGGTGGCTACACAT+TGG 0.533164 8.4:-39275845 MS.gene99980:CDS
GGCGCAACCTAGAAGACAAA+CGG 0.540704 8.4:+39276534 None:intergenic
ATATGACACAGAACTGAATC+CGG 0.542257 8.4:+39276599 None:intergenic
GTTGTTGCAGCTCATGAAGA+AGG 0.544005 8.4:+39276250 None:intergenic
ATGTGTAGCCACCAAGATGA+AGG 0.546460 8.4:+39275848 None:intergenic
TCTCCAAATCTTCGAGCTTG+GGG 0.548834 8.4:+39275959 None:intergenic
AGATACACCTTCTTCAGTGA+TGG 0.549162 8.4:+39276027 None:intergenic
AAGTCGAATGTATTGACATG+TGG 0.554066 8.4:-39276349 MS.gene99980:CDS
CGACAGAATAAGTAGTCTAC+CGG 0.557714 8.4:-39276618 MS.gene99980:CDS
CCTAGAAGACAAACGGCTCA+TGG 0.560672 8.4:+39276541 None:intergenic
AAGCTTTCTCCAGCTTCAAG+AGG 0.569171 8.4:-39275906 MS.gene99980:CDS
CAACAGACGTTCTTGTTGGG+AGG 0.577111 8.4:+39276567 None:intergenic
AGATCAAGTTCTTCAAGGCG+GGG 0.579251 8.4:+39276308 None:intergenic
TTTATATCAGGAACCTGAAG+TGG 0.582063 8.4:+39275587 None:intergenic
CAGCTCATGAAGAAGGAAGG+AGG 0.585175 8.4:+39276257 None:intergenic
GTCACGTCATTCAACATCAA+AGG 0.588332 8.4:-39275698 MS.gene99980:intron
AGCTCATGAAGAAGGAAGGA+GGG 0.594219 8.4:+39276258 None:intergenic
GATCAAGTTCTTCAAGGCGG+GGG 0.597189 8.4:+39276309 None:intergenic
TTTGAATGTGGCGCGATCTG+CGG 0.599793 8.4:+39276417 None:intergenic
ACTACTTATTCTGTCGATGT+GGG 0.610730 8.4:+39276625 None:intergenic
TTGCAGCTCATGAAGAAGGA+AGG 0.613912 8.4:+39276254 None:intergenic
AGTCGAATGTATTGACATGT+GGG 0.623434 8.4:-39276348 MS.gene99980:CDS
CCTGAAGATCTTTCCAGAGG+TGG 0.623862 8.4:+39276513 None:intergenic
AGATTTGGAGATTTATTGTG+TGG 0.626661 8.4:-39275947 MS.gene99980:CDS
AATGTGGCGCGATCTGCGGA+GGG 0.630677 8.4:+39276421 None:intergenic
TAAATTACCTGAGGGAAACG+AGG 0.631468 8.4:+39276228 None:intergenic
AGCAATCGAAGCCAAGATCG+TGG 0.645548 8.4:+39276480 None:intergenic
AAACTTTGCAGTCTATCTGG+TGG 0.646236 8.4:-39276090 MS.gene99980:intron
TGCATGGCGACTACGAATGG+AGG 0.648797 8.4:-39276650 MS.gene99980:CDS
TTATCATGCCAATTTAGGAG+CGG 0.653770 8.4:+39275027 None:intergenic
AGAATATTTGAGTTAAAACA+GGG 0.665389 8.4:+39275521 None:intergenic
TCTCATAATCAATAGCCACA+AGG 0.678177 8.4:-39275462 MS.gene99980:intron
TCATGAAGAAGGAAGGAGGG+AGG 0.688744 8.4:+39276261 None:intergenic
AGGCAACTTTCACACTATGG+CGG 0.690913 8.4:-39275815 MS.gene99980:CDS
TGAGATCAAGTTCTTCAAGG+CGG 0.698198 8.4:+39276306 None:intergenic
ACACCTGAAGATCTTTCCAG+AGG 0.719122 8.4:+39276510 None:intergenic
GAAGGTGTATCTAAGCACAA+CGG 0.731831 8.4:-39276016 MS.gene99980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAATTTAAAGAAGAAT+TGG + chr8.4:39275533-39275552 None:intergenic 10.0%
!! AATTAATTTAAAGAAGAATT+GGG + chr8.4:39275532-39275551 None:intergenic 10.0%
!! CTTTATATATAAATATAGTT+TGG - chr8.4:39276020-39276039 MS.gene99980:CDS 10.0%
!! ATTAATTTAAAGAAGAATTG+GGG + chr8.4:39275531-39275550 None:intergenic 15.0%
!!! ATTCTTTATTATCATTTTGA+AGG + chr8.4:39275949-39275968 None:intergenic 15.0%
!! AATTCTGGATAATTTATATC+AGG + chr8.4:39276105-39276124 None:intergenic 20.0%
!! GTTTAAATTAAATTACCTGA+GGG + chr8.4:39275460-39275479 None:intergenic 20.0%
!!! AATTTTTAATCCTACGAAAT+TGG - chr8.4:39275548-39275567 MS.gene99980:CDS 20.0%
!!! AGAATATTTGAGTTAAAACA+GGG + chr8.4:39276159-39276178 None:intergenic 20.0%
! AACAAATTCACTTTGACAAT+AGG + chr8.4:39275909-39275928 None:intergenic 25.0%
! AGTTTATTTGAATCCATCTT+TGG - chr8.4:39276255-39276274 MS.gene99980:CDS 25.0%
! ATGACTCGAAAATATATTTG+TGG + chr8.4:39276317-39276336 None:intergenic 25.0%
! ATTGATATTGTGATATTAGC+TGG + chr8.4:39275482-39275501 None:intergenic 25.0%
! CATCAAATTCATAGATGAAA+TGG + chr8.4:39275290-39275309 None:intergenic 25.0%
! GGTTTAAATTAAATTACCTG+AGG + chr8.4:39275461-39275480 None:intergenic 25.0%
!! CAGAATATTTGAGTTAAAAC+AGG + chr8.4:39276160-39276179 None:intergenic 25.0%
!! TGTTCTAAATAGCAAAATTC+TGG + chr8.4:39276120-39276139 None:intergenic 25.0%
!!! CTGTTTTAACTCAAATATTC+TGG - chr8.4:39276158-39276177 MS.gene99980:intron 25.0%
AAAAAAAGCTGAGAAGTTTG+CGG + chr8.4:39276548-39276567 None:intergenic 30.0%
AGATTTGGAGATTTATTGTG+TGG - chr8.4:39275730-39275749 MS.gene99980:CDS 30.0%
ATCTGGTGGTAAATACAATA+TGG - chr8.4:39275601-39275620 MS.gene99980:intron 30.0%
TTTGAAGGTGATTGAGAATA+GGG + chr8.4:39275934-39275953 None:intergenic 30.0%
! TGAAATGGAACTTTTGAATG+TGG + chr8.4:39275275-39275294 None:intergenic 30.0%
!! GTAGGAAAGGTTCTAAAAAA+CGG - chr8.4:39276354-39276373 MS.gene99980:CDS 30.0%
!! TTTTGAAGGTGATTGAGAAT+AGG + chr8.4:39275935-39275954 None:intergenic 30.0%
AAATTGGTGAACTCATGTGT+TGG - chr8.4:39275564-39275583 MS.gene99980:CDS 35.0%
ACACCAATGATAATTTCGCT+TGG - chr8.4:39275801-39275820 MS.gene99980:CDS 35.0%
ACTACTTATTCTGTCGATGT+GGG + chr8.4:39275055-39275074 None:intergenic 35.0%
AGAAATGTGAACAAACCTTG+TGG + chr8.4:39276233-39276252 None:intergenic 35.0%
AGTCCAAGCGAAATTATCAT+TGG + chr8.4:39275807-39275826 None:intergenic 35.0%
ATACATAGACAGACCAAAGA+TGG + chr8.4:39276271-39276290 None:intergenic 35.0%
ATATGACACAGAACTGAATC+CGG + chr8.4:39275081-39275100 None:intergenic 35.0%
GAAAGAACAAAAGATGAAGC+TGG + chr8.4:39276619-39276638 None:intergenic 35.0%
TATACAATGATGGCAAGTGA+TGG + chr8.4:39276060-39276079 None:intergenic 35.0%
TCTAAAAAACGGAACGTTAC+CGG - chr8.4:39276365-39276384 MS.gene99980:CDS 35.0%
TCTCATAATCAATAGCCACA+AGG - chr8.4:39276215-39276234 MS.gene99980:intron 35.0%
TGATGAGATCAAGTTCTTCA+AGG + chr8.4:39275377-39275396 None:intergenic 35.0%
TTGGAGATTTATTGTGTGGA+TGG - chr8.4:39275734-39275753 MS.gene99980:CDS 35.0%
TTTATATCAGGAACCTGAAG+TGG + chr8.4:39276093-39276112 None:intergenic 35.0%
! AAGTCGAATGTATTGACATG+TGG - chr8.4:39275328-39275347 MS.gene99980:intron 35.0%
! AGTCGAATGTATTGACATGT+GGG - chr8.4:39275329-39275348 MS.gene99980:intron 35.0%
AAACTTTGCAGTCTATCTGG+TGG - chr8.4:39275587-39275606 MS.gene99980:CDS 40.0%
AACCTTTCCTACCAGTAACA+TGG + chr8.4:39276346-39276365 None:intergenic 40.0%
AATCTCCAAATCTTCGAGCT+TGG + chr8.4:39275723-39275742 None:intergenic 40.0%
AGATACACCTTCTTCAGTGA+TGG + chr8.4:39275653-39275672 None:intergenic 40.0%
ATCTCCAAATCTTCGAGCTT+GGG + chr8.4:39275722-39275741 None:intergenic 40.0%
CATGAGTTCACCAATTTCGT+AGG + chr8.4:39275561-39275580 None:intergenic 40.0%
CCACTGCATGTATACAATGA+TGG + chr8.4:39276070-39276089 None:intergenic 40.0%
CCATCATTGTATACATGCAG+TGG - chr8.4:39276067-39276086 MS.gene99980:CDS 40.0%
CGACAGAATAAGTAGTCTAC+CGG - chr8.4:39275059-39275078 MS.gene99980:CDS 40.0%
CTAAAAAACGGAACGTTACC+GGG - chr8.4:39276366-39276385 MS.gene99980:CDS 40.0%
CTGGCTGAATTGAATCAAAG+AGG + chr8.4:39276600-39276619 None:intergenic 40.0%
GACTACTTATTCTGTCGATG+TGG + chr8.4:39275056-39275075 None:intergenic 40.0%
GCAATTTCCATCACTGAAGA+AGG - chr8.4:39275643-39275662 MS.gene99980:intron 40.0%
GTATTTCAACCTCTTGAAGC+TGG + chr8.4:39275783-39275802 None:intergenic 40.0%
GTCACGTCATTCAACATCAA+AGG - chr8.4:39275979-39275998 MS.gene99980:CDS 40.0%
GTCATCTTGTTCCATGTTAC+TGG - chr8.4:39276332-39276351 MS.gene99980:CDS 40.0%
TAAATTACCTGAGGGAAACG+AGG + chr8.4:39275452-39275471 None:intergenic 40.0%
TCAATAGTTGCCTTTCTCTC+CGG - chr8.4:39275692-39275711 MS.gene99980:intron 40.0%
TCACAGAAAAAACGCCGTTA+CGG - chr8.4:39276462-39276481 MS.gene99980:CDS 40.0%
TCCTTGAAAACGCCCAAAAA+CGG + chr8.4:39276431-39276450 None:intergenic 40.0%
TCTTGTTCCATGTTACTGGT+AGG - chr8.4:39276336-39276355 MS.gene99980:CDS 40.0%
TGAGATCAAGTTCTTCAAGG+CGG + chr8.4:39275374-39275393 None:intergenic 40.0%
TGGAAACTTTGCAGTCTATC+TGG - chr8.4:39275584-39275603 MS.gene99980:CDS 40.0%
TTCCATGTTACTGGTAGGAA+AGG - chr8.4:39276341-39276360 MS.gene99980:CDS 40.0%
TTGCAACAGACGTTCTTGTT+GGG + chr8.4:39275116-39275135 None:intergenic 40.0%
TTGTGTGGATGGAATCAGTT+TGG - chr8.4:39275745-39275764 MS.gene99980:CDS 40.0%
! GAAGGTGTATCTAAGCACAA+CGG - chr8.4:39275661-39275680 MS.gene99980:intron 40.0%
AAAACGCCCAAAAACGGTAC+CGG + chr8.4:39276425-39276444 None:intergenic 45.0%
AAGCTTTCTCCAGCTTCAAG+AGG - chr8.4:39275771-39275790 MS.gene99980:CDS 45.0%
ACACCTGAAGATCTTTCCAG+AGG + chr8.4:39275170-39275189 None:intergenic 45.0%
AGATCAAGTTCTTCAAGGCG+GGG + chr8.4:39275372-39275391 None:intergenic 45.0%
AGCTCATGAAGAAGGAAGGA+GGG + chr8.4:39275422-39275441 None:intergenic 45.0%
AGGCAACTTTCACACTATGG+CGG - chr8.4:39275862-39275881 MS.gene99980:CDS 45.0%
ATGTGTAGCCACCAAGATGA+AGG + chr8.4:39275832-39275851 None:intergenic 45.0%
GAGATCAAGTTCTTCAAGGC+GGG + chr8.4:39275373-39275392 None:intergenic 45.0%
GCTTGGACTTACCTTCATCT+TGG - chr8.4:39275818-39275837 MS.gene99980:CDS 45.0%
GTTGCAACAGACGTTCTTGT+TGG + chr8.4:39275117-39275136 None:intergenic 45.0%
GTTGTTGCAGCTCATGAAGA+AGG + chr8.4:39275430-39275449 None:intergenic 45.0%
TCTCCAAATCTTCGAGCTTG+GGG + chr8.4:39275721-39275740 None:intergenic 45.0%
TGAGAAGTTTGCGGCAGTAA+CGG + chr8.4:39276539-39276558 None:intergenic 45.0%
TGCAGGCAACTTTCACACTA+TGG - chr8.4:39275859-39275878 MS.gene99980:CDS 45.0%
TGGACTTACCTTCATCTTGG+TGG - chr8.4:39275821-39275840 MS.gene99980:CDS 45.0%
TTGCAGCTCATGAAGAAGGA+AGG + chr8.4:39275426-39275445 None:intergenic 45.0%
! ATGCCGTTACCGTTTTGTAC+CGG + chr8.4:39276399-39276418 None:intergenic 45.0%
! CATCTTGGTGGCTACACATT+GGG - chr8.4:39275833-39275852 MS.gene99980:CDS 45.0%
! TCATCTTGGTGGCTACACAT+TGG - chr8.4:39275832-39275851 MS.gene99980:CDS 45.0%
!! ACCGTTTTTGGGCGTTTTCA+AGG - chr8.4:39276427-39276446 MS.gene99980:CDS 45.0%
ACGGAACGTTACCGGGATTT+CGG - chr8.4:39276373-39276392 MS.gene99980:CDS 50.0%
AGACGTTCTTGTTGGGAGGA+AGG + chr8.4:39275109-39275128 None:intergenic 50.0%
AGCAATCGAAGCCAAGATCG+TGG + chr8.4:39275200-39275219 None:intergenic 50.0%
ATACATGCAGTGGCCACTTC+AGG - chr8.4:39276077-39276096 MS.gene99980:CDS 50.0%
CAACAGACGTTCTTGTTGGG+AGG + chr8.4:39275113-39275132 None:intergenic 50.0%
CACCACATCCGCTCCTAAAT+TGG - chr8.4:39276642-39276661 MS.gene99980:CDS 50.0%
CAGCTCATGAAGAAGGAAGG+AGG + chr8.4:39275423-39275442 None:intergenic 50.0%
CCATGAGCCGTTTGTCTTCT+AGG - chr8.4:39275136-39275155 MS.gene99980:intron 50.0%
CCTAGAAGACAAACGGCTCA+TGG + chr8.4:39275139-39275158 None:intergenic 50.0%
CCTGAAGATCTTTCCAGAGG+TGG + chr8.4:39275167-39275186 None:intergenic 50.0%
GATCAAGTTCTTCAAGGCGG+GGG + chr8.4:39275371-39275390 None:intergenic 50.0%
GGATTTCGGCCGGTACAAAA+CGG - chr8.4:39276387-39276406 MS.gene99980:CDS 50.0%
GGCGCAACCTAGAAGACAAA+CGG + chr8.4:39275146-39275165 None:intergenic 50.0%
GTGTTCTGCTTCCACGATCT+TGG - chr8.4:39275186-39275205 MS.gene99980:intron 50.0%
TCATGAAGAAGGAAGGAGGG+AGG + chr8.4:39275419-39275438 None:intergenic 50.0%
TGCCAATTTAGGAGCGGATG+TGG + chr8.4:39276647-39276666 None:intergenic 50.0%
TTTGAATGTGGCGCGATCTG+CGG + chr8.4:39275263-39275282 None:intergenic 50.0%
! AAAACGGTAACGGCATCCTC+CGG - chr8.4:39276403-39276422 MS.gene99980:CDS 50.0%
! CCACCTCTGGAAAGATCTTC+AGG - chr8.4:39275164-39275183 MS.gene99980:intron 50.0%
!! CATCCTCCGGTACCGTTTTT+GGG - chr8.4:39276416-39276435 MS.gene99980:CDS 50.0%
!! TTTTGTACCGGCCGAAATCC+CGG + chr8.4:39276387-39276406 None:intergenic 50.0%
AACACCGTAACGGCGTTTCG+CGG - chr8.4:39276494-39276513 MS.gene99980:CDS 55.0%
AACGTTACCGGGATTTCGGC+CGG - chr8.4:39276377-39276396 MS.gene99980:CDS 55.0%
ATGTGGGTCCGTTCTGCCAT+TGG - chr8.4:39275345-39275364 MS.gene99980:intron 55.0%
CGGCCGGTACAAAACGGTAA+CGG - chr8.4:39276393-39276412 MS.gene99980:CDS 55.0%
CTGCCCCAAGCTCGAAGATT+TGG - chr8.4:39275715-39275734 MS.gene99980:CDS 55.0%
GCAACAACCTCGTTTCCCTC+AGG - chr8.4:39275442-39275461 MS.gene99980:intron 55.0%
GCCGCGACAAAACACCGTAA+CGG - chr8.4:39276484-39276503 MS.gene99980:CDS 55.0%
! GCATCCTCCGGTACCGTTTT+TGG - chr8.4:39276415-39276434 MS.gene99980:CDS 55.0%
! GCCGTTACGGTGTTTTGTCG+CGG + chr8.4:39276488-39276507 None:intergenic 55.0%
! GGCTACACATTGGGCATTGC+AGG - chr8.4:39275842-39275861 MS.gene99980:CDS 55.0%
! GTGTTTTGTCGCGGCCGTAA+CGG + chr8.4:39276479-39276498 None:intergenic 55.0%
AATGTGGCGCGATCTGCGGA+GGG + chr8.4:39275259-39275278 None:intergenic 60.0%
ATCTTCGAGCTTGGGGCAGC+CGG + chr8.4:39275714-39275733 None:intergenic 60.0%
CTTCTAGGTTGCGCCACCTC+TGG - chr8.4:39275151-39275170 MS.gene99980:intron 60.0%
TTCTTCAAGGCGGGGGCCAA+TGG + chr8.4:39275364-39275383 None:intergenic 60.0%
! ACGCCCAAAAACGGTACCGG+AGG + chr8.4:39276422-39276441 None:intergenic 60.0%
! ACGGCGTTTTGCGGCCGTAA+CGG + chr8.4:39276520-39276539 None:intergenic 60.0%
! GCGGCAGTAACGGCGTTTTG+CGG + chr8.4:39276529-39276548 None:intergenic 60.0%
ACGGCCGCGAAACGCCGTTA+CGG + chr8.4:39276501-39276520 None:intergenic 65.0%
ACGGCGTTTCGCGGCCGTTA+CGG - chr8.4:39276503-39276522 MS.gene99980:CDS 65.0%
CTTGGGGCAGCCGGAGAGAA+AGG + chr8.4:39275705-39275724 None:intergenic 65.0%
GAATGTGGCGCGATCTGCGG+AGG + chr8.4:39275260-39275279 None:intergenic 65.0%
GGCGGGGGCCAATGGCAGAA+CGG + chr8.4:39275356-39275375 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 39275030 39276669 39275030 ID=MS.gene99980
chr8.4 mRNA 39275030 39276669 39275030 ID=MS.gene99980.t1;Parent=MS.gene99980
chr8.4 exon 39276236 39276669 39276236 ID=MS.gene99980.t1.exon1;Parent=MS.gene99980.t1
chr8.4 CDS 39276236 39276669 39276236 ID=cds.MS.gene99980.t1;Parent=MS.gene99980.t1
chr8.4 exon 39275699 39276101 39275699 ID=MS.gene99980.t1.exon2;Parent=MS.gene99980.t1
chr8.4 CDS 39275699 39276101 39275699 ID=cds.MS.gene99980.t1;Parent=MS.gene99980.t1
chr8.4 exon 39275463 39275612 39275463 ID=MS.gene99980.t1.exon3;Parent=MS.gene99980.t1
chr8.4 CDS 39275463 39275612 39275463 ID=cds.MS.gene99980.t1;Parent=MS.gene99980.t1
chr8.4 exon 39275030 39275083 39275030 ID=MS.gene99980.t1.exon4;Parent=MS.gene99980.t1
chr8.4 CDS 39275030 39275083 39275030 ID=cds.MS.gene99980.t1;Parent=MS.gene99980.t1
Gene Sequence

>MS.gene99980

ATGGCGACTACGAATGGAGGCACCCACATCGACAGAATAAGTAGTCTACCGGATTCAGTTCTGTGTCATATCATGTCCTTCCTCCCAACAAGAACGTCTGTTGCAACCATGAGCCGTTTGTCTTCTAGGTTGCGCCACCTCTGGAAAGATCTTCAGGTGTTCTGCTTCCACGATCTTGGCTTCGATTGCTTCAAAAAGTTTGCATTTTTTGTGAACGCTGTGCTTGCCCTCCGCAGATCGCGCCACATTCAAAAGTTCCATTTCATCTATGAATTTGATGAACATGACAAGAAGTTTCAAGTCGAATGTATTGACATGTGGGTCCGTTCTGCCATTGGCCCCCGCCTTGAAGAACTTGATCTCATCATCGTTGAAGCTGATTTAAGCCTCCCTCCTTCCTTCTTCATGAGCTGCAACAACCTCGTTTCCCTCAGGTAATTTAATTTAAACCAGCTAATATCACAATATCAATACAAACTAATTAGTATTTATTACTAACCCCAATTCTTCTTTAAATTAATTTTTAATCCTACGAAATTGGTGAACTCATGTGTTGGAAACTTTGCAGTCTATCTGGTGGTAAATACAATATGGAGATTAAACATTCTTTAGTGCAATTTCCATCACTGAAGAAGGTGTATCTAAGCACAACGGATTCATATTCAATAGTTGCCTTTCTCTCCGGCTGCCCCAAGCTCGAAGATTTGGAGATTTATTGTGTGGATGGAATCAGTTTGGCTAAAGCTTTCTCCAGCTTCAAGAGGTTGAAATACACCAATGATAATTTCGCTTGGACTTACCTTCATCTTGGTGGCTACACATTGGGCATTGCAGGCAACTTTCACACTATGGCGGAAGCATTTCTTGATGTCTTTTCCTATTGTCAAAGTGAATTTGTTGACCCTATTCTCAATCACCTTCAAAATGATAATAAAGAATTATATCTATTGTCACGTCATTCAACATCAAAGGTGAAGATTTATTTTTATGCTTTATATATAAATATAGTTTGGTACTAATATTATTTCCATCACTTGCCATCATTGTATACATGCAGTGGCCACTTCAGGTTCCTGATATAAATTATCCAGAATTTTGCTATTTAGAACATCTAAAGTTCAGTCTTCCCTGTTTTAACTCAAATATTCTGGTCAACGTGCTTGAGAAATGTCATGAGCTTAAAGTTCTCATAATCAATAGCCACAAGGTTTGTTCACATTTCTCAAGTTTATTTGAATCCATCTTTGGTCTGTCTATGTATGCATGTATGTATCAATTTGTAAACCACAAATATATTTTCGAGTCATCTTGTTCCATGTTACTGGTAGGAAAGGTTCTAAAAAACGGAACGTTACCGGGATTTCGGCCGGTACAAAACGGTAACGGCATCCTCCGGTACCGTTTTTGGGCGTTTTCAAGGAGTGAACAAAAATCACAGAAAAAACGCCGTTACGGCCGCGACAAAACACCGTAACGGCGTTTCGCGGCCGTTACGGCCGCAAAACGCCGTTACTGCCGCAAACTTCTCAGCTTTTTTTTCTCTGCGACTTCGTTGTTACTTTTGCTTCCTCTTTGATTCAATTCAGCCAGCTTCATCTTTTGTTCTTTCTAACACCACATCCGCTCCTAAATTGGCATGA
Protein sequence

>MS.gene99980.t1

MATTNGGTHIDRISSLPDSVLCHIMSFLPTRTSVATMSRLSSRLRHLWKDLQVFCFHDLGFDCFKKFAFFVNAVLALRRSRHIQKFHFIYEFDEHDKKFQVECIDMWVRSAIGPRLEELDLIIVEADLSLPPSFFMSCNNLVSLSLSGGKYNMEIKHSLVQFPSLKKVYLSTTDSYSIVAFLSGCPKLEDLEIYCVDGISLAKAFSSFKRLKYTNDNFAWTYLHLGGYTLGIAGNFHTMAEAFLDVFSYCQSEFVDPILNHLQNDNKELYLLSRHSTSKWPLQVPDINYPEFCYLEHLKFSLPCFNSNILVNVLEKCHELKVLIINSHKPASSFVLSNTTSAPKLA