AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055883

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055883.t1 MTR_8g062300 49.878 409 148 12 1 372 1 389 3.36e-111 334
MS.gene055883.t1 MTR_8g070950 50.900 389 143 11 1 378 1 352 2.94e-105 318
MS.gene055883.t1 MTR_8g062080 49.242 396 150 13 1 378 1 363 8.28e-100 304
MS.gene055883.t1 MTR_8g062310 47.091 361 150 9 21 378 24 346 3.36e-95 291
MS.gene055883.t1 MTR_8g062010 50.418 359 141 10 22 370 9 340 2.14e-91 282
MS.gene055883.t1 MTR_4g050250 44.363 408 155 14 9 373 8 386 6.49e-87 270
MS.gene055883.t1 MTR_8g058965 43.276 409 154 12 1 372 1 368 4.25e-82 259
MS.gene055883.t1 MTR_8g469020 41.814 397 163 12 19 378 4 369 2.06e-81 257
MS.gene055883.t1 MTR_8g467790 42.188 384 133 11 21 378 147 467 1.65e-75 243
MS.gene055883.t1 MTR_2g083840 36.480 392 195 12 4 370 2 364 5.61e-56 191
MS.gene055883.t1 MTR_8g062270 36.290 372 181 14 21 365 15 357 3.74e-52 181
MS.gene055883.t1 MTR_8g468810 36.543 405 191 18 3 373 2 374 2.68e-50 174
MS.gene055883.t1 MTR_8g468810 36.318 402 190 18 3 370 2 371 4.30e-48 170
MS.gene055883.t1 MTR_8g467800 36.649 382 195 15 9 370 5 359 1.02e-46 166
MS.gene055883.t1 MTR_4g032730 34.574 376 198 11 21 375 14 362 1.95e-46 166
MS.gene055883.t1 MTR_2g086020 56.774 155 61 3 48 201 1 150 5.08e-46 160
MS.gene055883.t1 MTR_2g086020 77.966 59 13 0 320 378 175 233 2.17e-16 79.7
MS.gene055883.t1 MTR_8g058970 34.390 410 201 16 3 370 2 385 5.62e-43 156
MS.gene055883.t1 MTR_8g445780 37.018 389 190 17 1 372 1 351 1.85e-42 153
MS.gene055883.t1 MTR_8g014260 35.176 398 195 19 1 370 1 363 1.10e-40 150
MS.gene055883.t1 MTR_8g468810 39.062 320 144 15 3 293 2 299 1.21e-40 147
MS.gene055883.t1 MTR_8g468810 39.062 320 144 15 3 293 2 299 1.35e-40 146
MS.gene055883.t1 MTR_8g468810 39.062 320 144 15 3 293 2 299 1.50e-40 147
MS.gene055883.t1 MTR_8g468840 34.091 396 202 15 1 372 1 361 2.23e-40 149
MS.gene055883.t1 MTR_5g092960 34.115 384 197 9 18 371 15 372 4.40e-40 147
MS.gene055883.t1 MTR_5g092960 34.026 385 196 10 18 371 15 372 4.76e-39 145
MS.gene055883.t1 MTR_8g468760 33.424 368 172 11 15 362 2 316 1.53e-35 135
MS.gene055883.t1 MTR_8g467800 38.562 306 150 16 9 293 5 293 6.57e-35 132
MS.gene055883.t1 MTR_8g014160 33.333 279 147 11 22 277 41 303 1.60e-25 107
MS.gene055883.t1 MTR_8g077460 38.053 226 111 11 22 233 10 220 8.33e-23 99.4
MS.gene055883.t1 MTR_5g021550 41.279 172 71 10 5 159 4 162 5.64e-22 92.0
MS.gene055883.t1 MTR_8g014290 38.608 158 71 6 22 160 10 160 1.71e-20 87.8
MS.gene055883.t1 MTR_7g056447 35.610 205 112 10 18 218 5 193 4.65e-17 81.3
MS.gene055883.t1 MTR_7g032380 34.634 205 105 9 19 213 8 193 9.06e-17 81.3
MS.gene055883.t1 MTR_1g041245 35.749 207 108 10 19 217 8 197 1.43e-16 81.3
MS.gene055883.t1 MTR_4g093690 31.718 227 122 12 3 217 2 207 1.78e-16 79.3
MS.gene055883.t1 MTR_5g073420 32.227 211 122 9 13 215 2 199 6.32e-16 79.0
MS.gene055883.t1 MTR_7g098510 32.569 218 123 11 1 213 1 199 1.39e-15 78.2
MS.gene055883.t1 MTR_4g024710 32.110 218 125 7 168 376 63 266 2.04e-15 77.4
MS.gene055883.t1 MTR_8g085430 32.558 215 115 8 13 213 2 200 6.23e-15 75.5
MS.gene055883.t1 MTR_2g007500 33.498 203 99 9 20 213 12 187 8.60e-15 75.1
MS.gene055883.t1 MTR_5g061680 31.429 210 121 8 13 214 2 196 1.11e-14 74.7
MS.gene055883.t1 MTR_3g107160 28.216 241 152 8 18 251 6 232 1.79e-14 74.7
MS.gene055883.t1 MTR_4g024720 39.130 138 65 7 1 130 1 127 4.07e-14 71.2
MS.gene055883.t1 MTR_3g107100 29.577 213 123 11 22 224 11 206 6.53e-14 73.2
MS.gene055883.t1 MTR_7g115270 34.703 219 107 12 19 219 9 209 7.33e-14 73.2
MS.gene055883.t1 MTR_4g056020 32.524 206 102 9 22 211 9 193 8.63e-14 73.2
MS.gene055883.t1 MTR_4g024770 53.846 78 28 4 1 78 8 77 1.82e-13 65.9
MS.gene055883.t1 MTR_7g034110 45.679 81 41 2 19 99 8 85 6.02e-13 69.7
MS.gene055883.t1 MTR_1g095750 41.667 84 47 2 19 101 43 125 7.68e-13 65.9
MS.gene055883.t1 MTR_5g008950 31.122 196 109 8 24 213 1 176 8.64e-13 69.7
MS.gene055883.t1 MTR_3g099830 31.441 229 121 11 11 228 1 204 1.62e-12 68.6
MS.gene055883.t1 MTR_1089s0010 30.542 203 114 8 21 214 7 191 2.80e-12 68.2
MS.gene055883.t1 MTR_7g056430 33.099 142 84 5 22 162 9 140 4.73e-12 68.2
MS.gene055883.t1 MTR_7g056420 33.099 142 84 5 22 162 9 140 5.10e-12 68.2
MS.gene055883.t1 MTR_6g044560 26.280 293 153 9 15 270 171 437 5.79e-12 67.8
MS.gene055883.t1 MTR_3g108150 32.000 200 112 9 19 213 10 190 1.18e-11 66.2
MS.gene055883.t1 MTR_3g105060 26.699 206 139 5 19 223 11 205 1.31e-11 65.9
MS.gene055883.t1 MTR_5g080710 28.495 372 213 18 13 366 24 360 1.52e-11 66.2
MS.gene055883.t1 MTR_7g033990 29.353 201 122 8 20 213 9 196 1.86e-11 65.9
MS.gene055883.t1 MTR_6g040270 31.313 198 113 8 24 214 10 191 1.90e-11 65.5
MS.gene055883.t1 MTR_6g044572 26.481 287 148 9 21 270 1 261 1.98e-11 65.5
MS.gene055883.t1 MTR_2g008310 32.836 201 109 9 21 213 1 183 2.61e-11 65.1
MS.gene055883.t1 MTR_3g082890 35.252 139 75 5 4 139 77 203 2.96e-11 65.5
MS.gene055883.t1 MTR_5g084070 27.248 367 220 14 12 366 3 334 5.27e-11 64.3
MS.gene055883.t1 MTR_7g034115 31.731 208 119 8 19 219 8 199 5.56e-11 63.9
MS.gene055883.t1 MTR_5g058100 30.000 220 123 10 15 222 2 202 5.57e-11 63.9
MS.gene055883.t1 MTR_7g055943 25.725 276 172 12 21 279 39 298 7.33e-11 63.9
MS.gene055883.t1 MTR_5g083890 29.630 216 121 10 18 212 2 207 9.76e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055883.t1 AT4G13965 34.673 199 111 7 21 216 9 191 8.92e-16 79.0
MS.gene055883.t1 AT3G26922 28.315 279 165 9 22 295 14 262 9.19e-16 77.8
MS.gene055883.t1 AT3G26922 28.315 279 165 9 22 295 14 262 9.19e-16 77.8
MS.gene055883.t1 AT4G26340 28.571 294 165 11 21 293 1 270 1.82e-15 78.2
MS.gene055883.t1 AT1G78760 29.236 301 150 15 9 281 8 273 1.91e-15 78.2
MS.gene055883.t1 AT4G15060 31.373 204 112 8 21 213 25 211 1.46e-13 72.4
MS.gene055883.t1 AT4G15060 31.373 204 112 8 21 213 171 357 1.60e-13 72.4
MS.gene055883.t1 AT2G26860 29.596 223 129 8 21 233 1 205 1.70e-13 72.0
MS.gene055883.t1 AT2G26860 29.596 223 129 8 21 233 1 205 1.70e-13 72.0
MS.gene055883.t1 AT5G56440 32.663 199 109 9 21 211 1 182 1.92e-13 72.0
MS.gene055883.t1 AT4G10400 26.740 273 166 10 21 278 1 254 3.33e-13 71.2
MS.gene055883.t1 AT4G10400 26.740 273 166 10 21 278 1 254 3.33e-13 71.2
MS.gene055883.t1 AT4G10400 26.740 273 166 10 21 278 1 254 3.33e-13 71.2
MS.gene055883.t1 AT1G16930 26.948 308 172 12 1 288 1 275 1.13e-12 69.7
MS.gene055883.t1 AT1G16930 26.948 308 172 12 1 288 1 275 1.13e-12 69.7
MS.gene055883.t1 AT1G16930 26.948 308 172 12 1 288 1 275 1.13e-12 69.7
MS.gene055883.t1 AT3G58940 28.372 215 116 9 21 213 1 199 2.25e-12 68.9
MS.gene055883.t1 AT3G58940 28.372 215 116 9 21 213 1 199 2.25e-12 68.9
MS.gene055883.t1 AT4G00315 31.343 201 117 9 21 213 1 188 2.80e-12 68.6
MS.gene055883.t1 AT1G55030 29.439 214 121 10 8 211 1 194 3.18e-12 68.2
MS.gene055883.t1 AT1G55030 29.798 198 113 9 20 211 7 184 3.20e-12 68.2
MS.gene055883.t1 AT4G13985 26.948 308 182 14 4 287 2 290 4.93e-12 67.8
MS.gene055883.t1 AT1G69630 26.712 292 182 8 1 278 1 274 7.99e-12 67.0
MS.gene055883.t1 AT5G35995 28.319 226 123 7 22 233 5 205 1.73e-11 65.9
MS.gene055883.t1 AT5G35995 28.319 226 123 7 22 233 5 205 1.73e-11 65.9
MS.gene055883.t1 AT1G13780 29.018 224 128 10 19 228 7 213 2.75e-11 64.7
MS.gene055883.t1 AT1G13780 29.018 224 128 10 19 228 7 213 2.78e-11 65.5
MS.gene055883.t1 AT5G22700 31.120 241 140 9 22 257 43 262 2.90e-11 65.1
MS.gene055883.t1 AT5G22700 31.120 241 140 9 22 257 43 262 4.05e-11 65.1
MS.gene055883.t1 AT5G22700 31.120 241 140 9 22 257 6 225 4.71e-11 64.7
MS.gene055883.t1 AT5G22700 31.120 241 140 9 22 257 6 225 4.71e-11 64.7
MS.gene055883.t1 AT5G22700 31.120 241 140 9 22 257 6 225 4.71e-11 64.7
MS.gene055883.t1 AT5G22700 31.120 241 140 9 22 257 6 225 4.71e-11 64.7
MS.gene055883.t1 AT5G60610 31.481 216 118 9 21 230 1 192 4.82e-11 64.3
MS.gene055883.t1 AT5G60610 31.481 216 118 9 21 230 1 192 4.82e-11 64.3
MS.gene055883.t1 AT4G00160 27.734 256 155 9 22 267 16 251 5.40e-11 64.3
MS.gene055883.t1 AT1G78730 26.425 193 112 5 21 213 21 183 6.62e-11 63.9
MS.gene055883.t1 AT5G50270 25.000 292 178 9 21 291 1 272 9.74e-11 63.5

Find 85 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCAGGCTGGAGACTTTGC+AGG 0.207215 8.3:+42618225 MS.gene055883:CDS
GATCCACGCTTCAGCATTCT+TGG 0.265206 8.3:-42617849 None:intergenic
CAACTTTATCTCAATGTTGA+TGG 0.302931 8.3:+42617899 MS.gene055883:CDS
CGTTGGTAACTTCCGTAATA+TGG 0.311536 8.3:+42618396 MS.gene055883:CDS
GAAATTCACTTCTGGTATCT+TGG 0.314508 8.3:-42618486 None:intergenic
CGCCTTTGATCGCCTTCAAA+GGG 0.315839 8.3:+42617802 MS.gene055883:CDS
TGATGTAAATAGCGAAATTC+AGG 0.334520 8.3:-42618695 None:intergenic
TCATCTCTGGAAGGATCTTC+AGG 0.350215 8.3:+42617667 MS.gene055883:CDS
CAAGAACACAACCCCTTTGA+AGG 0.357310 8.3:-42617814 None:intergenic
ATGTTTCAACTCAAATCTTC+TGG 0.358687 8.3:+42618736 MS.gene055883:CDS
TCTCGCTGGCTGCCCCAGGC+TGG 0.373730 8.3:+42618213 MS.gene055883:CDS
TTCTAATACTGCAGTCTTCA+CGG 0.375556 8.3:+42618076 MS.gene055883:intron
GCTTCTATGAAATTCACTTC+TGG 0.378368 8.3:-42618494 None:intergenic
TTACTAATGATGAAACTGTT+TGG 0.390635 8.3:-42617724 None:intergenic
CATCCTCATAGTTAATTATG+AGG 0.414466 8.3:-42618516 None:intergenic
CTTGGATGAGGTTGAGAATA+GGG 0.417120 8.3:-42618468 None:intergenic
CTGCTCAACCTGTCGACGAC+TGG 0.430154 8.3:-42617554 None:intergenic
TGGGGAGGAAGGACATGATA+TGG 0.433088 8.3:-42617598 None:intergenic
CTTCAGTGATGGAAAATAAA+CGG 0.436856 8.3:-42618140 None:intergenic
TCTTTCTCGCTGGCTGCCCC+AGG 0.439455 8.3:+42618209 MS.gene055883:CDS
ACCTGCAAAGTCTCCAGCCT+GGG 0.440076 8.3:-42618226 None:intergenic
TCTTGGATGAGGTTGAGAAT+AGG 0.442368 8.3:-42618469 None:intergenic
TTTGAATGTTGCGGGATTTG+CGG 0.447214 8.3:-42617763 None:intergenic
TCGCCTTTGATCGCCTTCAA+AGG 0.447806 8.3:+42617801 MS.gene055883:CDS
GAAATGCTTCCACCATATTA+CGG 0.454353 8.3:-42618408 None:intergenic
CTCGCAGGTATCTTCATCTC+TGG 0.455203 8.3:+42617654 MS.gene055883:CDS
AATTCAGGATAATTTAGAAC+AGG 0.463615 8.3:-42618680 None:intergenic
GAGATAAAGTTGTTGAAGGT+GGG 0.465366 8.3:-42617888 None:intergenic
CACTTCTGGTATCTTGGATG+AGG 0.469527 8.3:-42618480 None:intergenic
AACATGCTTACTGATATTGT+TGG 0.475441 8.3:+42619462 MS.gene055883:CDS
AGTCAGTAAGTTGGCATAAG+TGG 0.475566 8.3:-42619505 None:intergenic
GGAAGGTGTCACTAGAACTT+TGG 0.483436 8.3:-42618287 None:intergenic
ACATGTCATCATCGGAAGAA+AGG 0.484670 8.3:-42618258 None:intergenic
TGCTCAACCTGTCGACGACT+GGG 0.488561 8.3:-42617553 None:intergenic
TGAGATAAAGTTGTTGAAGG+TGG 0.491370 8.3:-42617889 None:intergenic
CATTGAGATAAAGTTGTTGA+AGG 0.494116 8.3:-42617892 None:intergenic
GCACCAACCTCGTTTCCCTC+AGG 0.500754 8.3:+42617957 MS.gene055883:CDS
AGTTACTTTAATATAAATGA+AGG 0.511759 8.3:+42618355 MS.gene055883:CDS
TCTACAAATTCACTTTCAGC+CGG 0.514388 8.3:-42618445 None:intergenic
TTGTTGAAGGTGGGGTCCAA+TGG 0.515637 8.3:-42617879 None:intergenic
GCGTGGATCCTTGCTGCCAT+TGG 0.523474 8.3:+42617863 MS.gene055883:CDS
ATTTATATTAAAGTAACTCC+AGG 0.524406 8.3:-42618350 None:intergenic
AAGCCTCATAATTAACTATG+AGG 0.525772 8.3:+42618513 MS.gene055883:CDS
AAAGAAGAAAGTCAGTAAGT+TGG 0.528899 8.3:-42619514 None:intergenic
AATGCGACCCAGTCGTCGAC+AGG 0.531434 8.3:+42617546 MS.gene055883:CDS
AACCTGCAAAGTCTCCAGCC+TGG 0.539126 8.3:-42618227 None:intergenic
ACCCCTTTGAAGGCGATCAA+AGG 0.543073 8.3:-42617804 None:intergenic
CGATGAGCCTTGTCTCTCGC+AGG 0.545430 8.3:+42617639 MS.gene055883:CDS
TTTAGAACAGGTGCATGAGT+TGG 0.547228 8.3:-42618668 None:intergenic
GGATATCAAGCTGTTCGAGC+TGG 0.558732 8.3:-42618311 None:intergenic
AGATAAAGTTGTTGAAGGTG+GGG 0.559902 8.3:-42617887 None:intergenic
CCGCTGATGACAATTTCACC+TGG 0.562804 8.3:+42618332 MS.gene055883:CDS
TGAATCGACGTCGATGGCGG+AGG 0.566196 8.3:+42617487 None:intergenic
ATATGGCATAACAACGAGTC+CGG 0.568277 8.3:-42617581 None:intergenic
AAGCTGTTCGAGCTGGAGGA+AGG 0.570594 8.3:-42618304 None:intergenic
CTACAAATTCACTTTCAGCC+GGG 0.571470 8.3:-42618444 None:intergenic
CGACAGGTTGAGCAGTCTAC+CGG 0.574240 8.3:+42617562 MS.gene055883:CDS
GAATGTTGCGGGATTTGCGG+AGG 0.575959 8.3:-42617760 None:intergenic
GTCTCTCGGCAATGATATTG+TGG 0.576609 8.3:+42618168 MS.gene055883:CDS
GGTCAAAGACATGTCATCAT+CGG 0.578513 8.3:-42618266 None:intergenic
TGTGGCATTGCTCAAGACAA+TGG 0.586966 8.3:-42618760 None:intergenic
AGAAGATTTGAGTTGAAACA+TGG 0.586977 8.3:-42618734 None:intergenic
GACAAGGCTCATCGTAGTAA+CGG 0.589535 8.3:-42617630 None:intergenic
AGAGACAACATCTTCAGTGA+TGG 0.590452 8.3:-42618151 None:intergenic
GATTTGAGTTGAAACATGGA+AGG 0.600986 8.3:-42618730 None:intergenic
TATCAAGCTGTTCGAGCTGG+AGG 0.602132 8.3:-42618308 None:intergenic
AATGATGAAACTGTTTGGAA+CGG 0.605943 8.3:-42617719 None:intergenic
TCACTGAAGATGTTGTCTCT+CGG 0.609046 8.3:+42618154 MS.gene055883:CDS
CAGGTATCTTCATCTCTGGA+AGG 0.616499 8.3:+42617658 MS.gene055883:CDS
GTTGCATCATTCGATGTCAA+AGG 0.618438 8.3:+42618549 MS.gene055883:CDS
TTACCTGAGGGAAACGAGGT+TGG 0.622908 8.3:-42617960 None:intergenic
GGTGGGGTCCAATGGCAGCA+AGG 0.624675 8.3:-42617871 None:intergenic
TGGTAACTTCCGTAATATGG+TGG 0.629703 8.3:+42618399 MS.gene055883:CDS
GATCAAAGGCGAAAGTGAGA+TGG 0.632265 8.3:-42617790 None:intergenic
GAAGATACCTGCGAGAGACA+AGG 0.633083 8.3:-42617646 None:intergenic
CAATTGATAAAGAGTGAACG+AGG 0.640528 8.3:-42617935 None:intergenic
TCTCATAATTCAGAGCAACA+AGG 0.643040 8.3:+42618793 MS.gene055883:CDS
CCAGGTGAAATTGTCATCAG+CGG 0.651525 8.3:-42618332 None:intergenic
AATCCAAGAATGCTGAAGCG+TGG 0.654776 8.3:+42617846 MS.gene055883:CDS
GCCTTTGATCGCCTTCAAAG+GGG 0.664858 8.3:+42617803 MS.gene055883:CDS
CCTGCAAAGTCTCCAGCCTG+GGG 0.668022 8.3:-42618225 None:intergenic
CTGTTTGGAACGGAATTGTG+TGG 0.688147 8.3:-42617709 None:intergenic
TTATGAGAACTTGAAGCATG+TGG 0.695487 8.3:-42618778 None:intergenic
GAAGTTACCTGAGGGAAACG+AGG 0.701057 8.3:-42617964 None:intergenic
AATTGATAAAGAGTGAACGA+GGG 0.746792 8.3:-42617934 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATATTTGTGTACAAA+TGG - chr8.3:42618886-42618905 None:intergenic 15.0%
!! AAATATAATTTGGATACATT+GGG - chr8.3:42619278-42619297 None:intergenic 15.0%
!! AATTTAATTCAAACATTCTT+TGG + chr8.3:42618831-42618850 MS.gene055883:intron 15.0%
!!! AGTTACTTTAATATAAATGA+AGG + chr8.3:42618355-42618374 MS.gene055883:CDS 15.0%
!!! TGAAATTTGCTAAATTTATA+AGG + chr8.3:42619420-42619439 MS.gene055883:intron 15.0%
!! AATATAATTTGGATACATTG+GGG - chr8.3:42619277-42619296 None:intergenic 20.0%
!! ATTTAATGTAGTACAAAGTA+AGG - chr8.3:42619339-42619358 None:intergenic 20.0%
!! ATTTATATTAAAGTAACTCC+AGG - chr8.3:42618353-42618372 None:intergenic 20.0%
!! CAAATAGACCAAATATAATT+TGG - chr8.3:42619288-42619307 None:intergenic 20.0%
!! CAAATATAATTTGGATACAT+TGG - chr8.3:42619279-42619298 None:intergenic 20.0%
!! CAATGTATCCAAATTATATT+TGG + chr8.3:42619277-42619296 MS.gene055883:intron 20.0%
!!! ACAAATATATTTTTGAGTCA+CGG + chr8.3:42618893-42618912 MS.gene055883:intron 20.0%
!!! ATGTTTTAAAATGTGTAGTT+TGG + chr8.3:42618596-42618615 MS.gene055883:intron 20.0%
!!! TGTTTTTGTTGATATTTCAA+TGG + chr8.3:42619105-42619124 MS.gene055883:intron 20.0%
!!! TTGTTAATCTTTTGATAGAA+CGG - chr8.3:42619147-42619166 None:intergenic 20.0%
! AAGATTAACAAATGTAACGA+AGG + chr8.3:42619156-42619175 MS.gene055883:intron 25.0%
! AGATTAACAAATGTAACGAA+GGG + chr8.3:42619157-42619176 MS.gene055883:intron 25.0%
! ATATAATTTGGATACATTGG+GGG - chr8.3:42619276-42619295 None:intergenic 25.0%
! TAATTCAAACATTCTTTGGT+CGG + chr8.3:42618835-42618854 MS.gene055883:intron 25.0%
! TTACTAATGATGAAACTGTT+TGG - chr8.3:42617727-42617746 None:intergenic 25.0%
!! AATTCAGGATAATTTAGAAC+AGG - chr8.3:42618683-42618702 None:intergenic 25.0%
!! TGTGTAACAATGTTTGATTT+AGG + chr8.3:42619377-42619396 MS.gene055883:intron 25.0%
!!! AAATGAAGGACGTTTTAAAT+TGG + chr8.3:42618369-42618388 MS.gene055883:CDS 25.0%
!!! AAGGTGAAGTTTTACTTTTA+TGG + chr8.3:42618568-42618587 MS.gene055883:intron 25.0%
!!! AATGAAGGACGTTTTAAATT+GGG + chr8.3:42618370-42618389 MS.gene055883:CDS 25.0%
!!! ATTTCTTGATGTTTTTTTCC+CGG + chr8.3:42618426-42618445 MS.gene055883:CDS 25.0%
AACATGCTTACTGATATTGT+TGG + chr8.3:42619462-42619481 MS.gene055883:CDS 30.0%
AAGCCTCATAATTAACTATG+AGG + chr8.3:42618513-42618532 MS.gene055883:CDS 30.0%
AATGATGAAACTGTTTGGAA+CGG - chr8.3:42617722-42617741 None:intergenic 30.0%
AATTGATAAAGAGTGAACGA+GGG - chr8.3:42617937-42617956 None:intergenic 30.0%
ACAAAGTAAGGATCTCATTT+AGG - chr8.3:42619327-42619346 None:intergenic 30.0%
ATATTCACATAGAAGGATCA+AGG + chr8.3:42619016-42619035 MS.gene055883:intron 30.0%
ATGTTTCAACTCAAATCTTC+TGG + chr8.3:42618736-42618755 MS.gene055883:CDS 30.0%
CAACTTTATCTCAATGTTGA+TGG + chr8.3:42617899-42617918 MS.gene055883:CDS 30.0%
CATCCTCATAGTTAATTATG+AGG - chr8.3:42618519-42618538 None:intergenic 30.0%
CATTGAGATAAAGTTGTTGA+AGG - chr8.3:42617895-42617914 None:intergenic 30.0%
TAAAAACAGAAGTTACCTGA+GGG - chr8.3:42617975-42617994 None:intergenic 30.0%
TGATCCTTCTATGTGAATAT+AGG - chr8.3:42619016-42619035 None:intergenic 30.0%
TGATGTAAATAGCGAAATTC+AGG - chr8.3:42618698-42618717 None:intergenic 30.0%
TTAAAAACAGAAGTTACCTG+AGG - chr8.3:42617976-42617995 None:intergenic 30.0%
TTTAATCCCATCTTAATTGC+AGG + chr8.3:42618003-42618022 MS.gene055883:intron 30.0%
! CATTTGCTGAATATATCTTG+CGG + chr8.3:42619056-42619075 MS.gene055883:intron 30.0%
! CTTCAGTGATGGAAAATAAA+CGG - chr8.3:42618143-42618162 None:intergenic 30.0%
! TTCAAAAGTGAAAGTGTGTT+TGG - chr8.3:42618047-42618066 None:intergenic 30.0%
!! AGAAGATTTGAGTTGAAACA+TGG - chr8.3:42618737-42618756 None:intergenic 30.0%
!! GTAGTAACGGAAATTTTTGT+GGG - chr8.3:42617620-42617639 None:intergenic 30.0%
!! TAGTAACGGAAATTTTTGTG+GGG - chr8.3:42617619-42617638 None:intergenic 30.0%
AGATAAAGTTGTTGAAGGTG+GGG - chr8.3:42617890-42617909 None:intergenic 35.0%
CAATTGATAAAGAGTGAACG+AGG - chr8.3:42617938-42617957 None:intergenic 35.0%
CACCTTAAGAAGATACATCT+GGG - chr8.3:42619210-42619229 None:intergenic 35.0%
CATCTGGGTCAAATTTAAGT+TGG - chr8.3:42619195-42619214 None:intergenic 35.0%
CTGACCTATATTCACATAGA+AGG + chr8.3:42619009-42619028 MS.gene055883:intron 35.0%
CTTCTATGTGAATATAGGTC+AGG - chr8.3:42619011-42619030 None:intergenic 35.0%
CTTCTTAAGGTGAAAACTCT+CGG + chr8.3:42619218-42619237 MS.gene055883:intron 35.0%
GAAATGCTTCCACCATATTA+CGG - chr8.3:42618411-42618430 None:intergenic 35.0%
GAAATTCACTTCTGGTATCT+TGG - chr8.3:42618489-42618508 None:intergenic 35.0%
GAGATAAAGTTGTTGAAGGT+GGG - chr8.3:42617891-42617910 None:intergenic 35.0%
GAGTATCCTGCAATTAAGAT+GGG - chr8.3:42618012-42618031 None:intergenic 35.0%
GCTTCTATGAAATTCACTTC+TGG - chr8.3:42618497-42618516 None:intergenic 35.0%
TATGTGAATATAGGTCAGGT+GGG - chr8.3:42619007-42619026 None:intergenic 35.0%
TCACCTTAAGAAGATACATC+TGG - chr8.3:42619211-42619230 None:intergenic 35.0%
TCTACAAATTCACTTTCAGC+CGG - chr8.3:42618448-42618467 None:intergenic 35.0%
TCTCATAATTCAGAGCAACA+AGG + chr8.3:42618793-42618812 MS.gene055883:CDS 35.0%
TGAGATAAAGTTGTTGAAGG+TGG - chr8.3:42617892-42617911 None:intergenic 35.0%
TGAGTATCCTGCAATTAAGA+TGG - chr8.3:42618013-42618032 None:intergenic 35.0%
TTATGAGAACTTGAAGCATG+TGG - chr8.3:42618781-42618800 None:intergenic 35.0%
TTCTAATACTGCAGTCTTCA+CGG + chr8.3:42618076-42618095 MS.gene055883:intron 35.0%
!! CGTAGTAACGGAAATTTTTG+TGG - chr8.3:42617621-42617640 None:intergenic 35.0%
!! GATTTGAGTTGAAACATGGA+AGG - chr8.3:42618733-42618752 None:intergenic 35.0%
!!! ATGGAAGTTTTGAATGTTGC+GGG - chr8.3:42617774-42617793 None:intergenic 35.0%
!!! GAATTAGTTTTCTTTCTCGC+TGG + chr8.3:42618199-42618218 MS.gene055883:CDS 35.0%
!!! GATGGAAGTTTTGAATGTTG+CGG - chr8.3:42617775-42617794 None:intergenic 35.0%
!!! TATATTTTTGAGTCACGGCT+TGG + chr8.3:42618898-42618917 MS.gene055883:intron 35.0%
AACAACCACCATTGAGAACA+TGG + chr8.3:42618944-42618963 MS.gene055883:intron 40.0%
ACATGTCATCATCGGAAGAA+AGG - chr8.3:42618261-42618280 None:intergenic 40.0%
AGAGACAACATCTTCAGTGA+TGG - chr8.3:42618154-42618173 None:intergenic 40.0%
ATATGGCATAACAACGAGTC+CGG - chr8.3:42617584-42617603 None:intergenic 40.0%
ATTTGGATACATTGGGGGTA+TGG - chr8.3:42619271-42619290 None:intergenic 40.0%
CTACAAATTCACTTTCAGCC+GGG - chr8.3:42618447-42618466 None:intergenic 40.0%
CTATGTGAATATAGGTCAGG+TGG - chr8.3:42619008-42619027 None:intergenic 40.0%
CTTGGATGAGGTTGAGAATA+GGG - chr8.3:42618471-42618490 None:intergenic 40.0%
GACCCAGATGTATCTTCTTA+AGG + chr8.3:42619205-42619224 MS.gene055883:intron 40.0%
GCTGAATATATCTTGCGGAA+CGG + chr8.3:42619061-42619080 MS.gene055883:intron 40.0%
GGTCAAAGACATGTCATCAT+CGG - chr8.3:42618269-42618288 None:intergenic 40.0%
GTTGCATCATTCGATGTCAA+AGG + chr8.3:42618549-42618568 MS.gene055883:CDS 40.0%
TCACTGAAGATGTTGTCTCT+CGG + chr8.3:42618154-42618173 MS.gene055883:CDS 40.0%
TCTTGGATGAGGTTGAGAAT+AGG - chr8.3:42618472-42618491 None:intergenic 40.0%
TGGTAACTTCCGTAATATGG+TGG + chr8.3:42618399-42618418 MS.gene055883:CDS 40.0%
TTGTTATCGTGTATGTGCAG+TGG + chr8.3:42618645-42618664 MS.gene055883:intron 40.0%
TTTGAATGTTGCGGGATTTG+CGG - chr8.3:42617766-42617785 None:intergenic 40.0%
! TAACGGAAATTTTTGTGGGG+AGG - chr8.3:42617616-42617635 None:intergenic 40.0%
! TTGGGGGTATGGAATTATGT+AGG - chr8.3:42619260-42619279 None:intergenic 40.0%
! TTTAGAACAGGTGCATGAGT+TGG - chr8.3:42618671-42618690 None:intergenic 40.0%
!! CGTTGGTAACTTCCGTAATA+TGG + chr8.3:42618396-42618415 MS.gene055883:CDS 40.0%
!! CGTTTTAAATTGGGCATCGT+TGG + chr8.3:42618379-42618398 MS.gene055883:CDS 40.0%
!! TCAGAAACTCTTGTTGACTC+CGG - chr8.3:42618973-42618992 None:intergenic 40.0%
AATCCAAGAATGCTGAAGCG+TGG + chr8.3:42617846-42617865 MS.gene055883:CDS 45.0%
CAAGAACACAACCCCTTTGA+AGG - chr8.3:42617817-42617836 None:intergenic 45.0%
CACTTCTGGTATCTTGGATG+AGG - chr8.3:42618483-42618502 None:intergenic 45.0%
CAGGTATCTTCATCTCTGGA+AGG + chr8.3:42617658-42617677 MS.gene055883:CDS 45.0%
CCAGGTGAAATTGTCATCAG+CGG - chr8.3:42618335-42618354 None:intergenic 45.0%
GACAAGGCTCATCGTAGTAA+CGG - chr8.3:42617633-42617652 None:intergenic 45.0%
GATCAAAGGCGAAAGTGAGA+TGG - chr8.3:42617793-42617812 None:intergenic 45.0%
GTCTCTCGGCAATGATATTG+TGG + chr8.3:42618168-42618187 MS.gene055883:CDS 45.0%
TGTGGCATTGCTCAAGACAA+TGG - chr8.3:42618763-42618782 None:intergenic 45.0%
! CTGTTTGGAACGGAATTGTG+TGG - chr8.3:42617712-42617731 None:intergenic 45.0%
! GGAAATTTTTGTGGGGAGGA+AGG - chr8.3:42617612-42617631 None:intergenic 45.0%
! TCATCTCTGGAAGGATCTTC+AGG + chr8.3:42617667-42617686 MS.gene055883:CDS 45.0%
!! GGAAGGTGTCACTAGAACTT+TGG - chr8.3:42618290-42618309 None:intergenic 45.0%
ACCATTGAGAACATGGCAGC+CGG + chr8.3:42618951-42618970 MS.gene055883:intron 50.0%
CCGCTGATGACAATTTCACC+TGG + chr8.3:42618332-42618351 MS.gene055883:CDS 50.0%
CGCCTTTGATCGCCTTCAAA+GGG + chr8.3:42617802-42617821 MS.gene055883:CDS 50.0%
CTCGCAGGTATCTTCATCTC+TGG + chr8.3:42617654-42617673 MS.gene055883:CDS 50.0%
GAAGATACCTGCGAGAGACA+AGG - chr8.3:42617649-42617668 None:intergenic 50.0%
GAAGTTACCTGAGGGAAACG+AGG - chr8.3:42617967-42617986 None:intergenic 50.0%
GATCCACGCTTCAGCATTCT+TGG - chr8.3:42617852-42617871 None:intergenic 50.0%
GCCTTTGATCGCCTTCAAAG+GGG + chr8.3:42617803-42617822 MS.gene055883:CDS 50.0%
GGATATCAAGCTGTTCGAGC+TGG - chr8.3:42618314-42618333 None:intergenic 50.0%
GTTCCTCCTACGAGCAACAA+GGG - chr8.3:42618927-42618946 None:intergenic 50.0%
TATCAAGCTGTTCGAGCTGG+AGG - chr8.3:42618311-42618330 None:intergenic 50.0%
TCGCCTTTGATCGCCTTCAA+AGG + chr8.3:42617801-42617820 MS.gene055883:CDS 50.0%
TGGGGAGGAAGGACATGATA+TGG - chr8.3:42617601-42617620 None:intergenic 50.0%
TGTTCCTCCTACGAGCAACA+AGG - chr8.3:42618928-42618947 None:intergenic 50.0%
TTACCTGAGGGAAACGAGGT+TGG - chr8.3:42617963-42617982 None:intergenic 50.0%
TTGTTGAAGGTGGGGTCCAA+TGG - chr8.3:42617882-42617901 None:intergenic 50.0%
! ACCCCTTTGAAGGCGATCAA+AGG - chr8.3:42617807-42617826 None:intergenic 50.0%
!! CTTGCGGAACGGACTTGTTT+TGG + chr8.3:42619072-42619091 MS.gene055883:intron 50.0%
AACCTGCAAAGTCTCCAGCC+TGG - chr8.3:42618230-42618249 None:intergenic 55.0%
AAGCTGTTCGAGCTGGAGGA+AGG - chr8.3:42618307-42618326 None:intergenic 55.0%
ACCTGCAAAGTCTCCAGCCT+GGG - chr8.3:42618229-42618248 None:intergenic 55.0%
GAATGTTGCGGGATTTGCGG+AGG - chr8.3:42617763-42617782 None:intergenic 55.0%
GGCTGCCATGTTCTCAATGG+TGG - chr8.3:42618952-42618971 None:intergenic 55.0%
TCCGGCTGCCATGTTCTCAA+TGG - chr8.3:42618955-42618974 None:intergenic 55.0%
TGCTCAACCTGTCGACGACT+GGG - chr8.3:42617556-42617575 None:intergenic 55.0%
TGGTGTCCCTTGTTGCTCGT+AGG + chr8.3:42618918-42618937 MS.gene055883:intron 55.0%
TGTCCCTTGTTGCTCGTAGG+AGG + chr8.3:42618921-42618940 MS.gene055883:intron 55.0%
! CGACAGGTTGAGCAGTCTAC+CGG + chr8.3:42617562-42617581 MS.gene055883:CDS 55.0%
AATGCGACCCAGTCGTCGAC+AGG + chr8.3:42617546-42617565 MS.gene055883:CDS 60.0%
CCTGCAAAGTCTCCAGCCTG+GGG - chr8.3:42618228-42618247 None:intergenic 60.0%
CTGCTCAACCTGTCGACGAC+TGG - chr8.3:42617557-42617576 None:intergenic 60.0%
GCACCAACCTCGTTTCCCTC+AGG + chr8.3:42617957-42617976 MS.gene055883:CDS 60.0%
GCGTGGATCCTTGCTGCCAT+TGG + chr8.3:42617863-42617882 MS.gene055883:CDS 60.0%
! CGATGAGCCTTGTCTCTCGC+AGG + chr8.3:42617639-42617658 MS.gene055883:CDS 60.0%
CCCCAGGCTGGAGACTTTGC+AGG + chr8.3:42618225-42618244 MS.gene055883:CDS 65.0%
GGTGGGGTCCAATGGCAGCA+AGG - chr8.3:42617874-42617893 None:intergenic 65.0%
! TCTTTCTCGCTGGCTGCCCC+AGG + chr8.3:42618209-42618228 MS.gene055883:CDS 65.0%
! TCTCGCTGGCTGCCCCAGGC+TGG + chr8.3:42618213-42618232 MS.gene055883:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 42617500 42619525 42617500 ID=MS.gene055883
chr8.3 mRNA 42617500 42619525 42617500 ID=MS.gene055883.t1;Parent=MS.gene055883
chr8.3 exon 42617500 42617978 42617500 ID=MS.gene055883.t1.exon1;Parent=MS.gene055883.t1
chr8.3 CDS 42617500 42617978 42617500 ID=cds.MS.gene055883.t1;Parent=MS.gene055883.t1
chr8.3 exon 42618090 42618570 42618090 ID=MS.gene055883.t1.exon2;Parent=MS.gene055883.t1
chr8.3 CDS 42618090 42618570 42618090 ID=cds.MS.gene055883.t1;Parent=MS.gene055883.t1
chr8.3 exon 42618665 42618814 42618665 ID=MS.gene055883.t1.exon3;Parent=MS.gene055883.t1
chr8.3 CDS 42618665 42618814 42618665 ID=cds.MS.gene055883.t1;Parent=MS.gene055883.t1
chr8.3 exon 42619442 42619525 42619442 ID=MS.gene055883.t1.exon4;Parent=MS.gene055883.t1
chr8.3 CDS 42619442 42619525 42619442 ID=cds.MS.gene055883.t1;Parent=MS.gene055883.t1
Gene Sequence

>MS.gene055883

ATGGCGGAGGAGTCAAGTATGATGAAACGACTGAAAGCACAAGAAGAATGCGACCCAGTCGTCGACAGGTTGAGCAGTCTACCGGACTCGTTGTTATGCCATATCATGTCCTTCCTCCCCACAAAAATTTCCGTTACTACGATGAGCCTTGTCTCTCGCAGGTATCTTCATCTCTGGAAGGATCTTCAGGTCTTGTATTTCGACTTTCACCACACAATTCCGTTCCAAACAGTTTCATCATTAGTAAACTCTGTTCTCGTCCTCCGCAAATCCCGCAACATTCAAAACTTCCATCTCACTTTCGCCTTTGATCGCCTTCAAAGGGGTTGTGTTCTTGAAAAGTTACAATCCAAGAATGCTGAAGCGTGGATCCTTGCTGCCATTGGACCCCACCTTCAACAACTTTATCTCAATGTTGATGGTTGTATAGATATCCCTCGTTCACTCTTTATCAATTGCACCAACCTCGTTTCCCTCAGGTAACTTCTGTTTTTAAATTAATTTTTAATCCCATCTTAATTGCAGGATACTCATGTTTTAATGTCCAAACACACTTTCACTTTTGAATCATACTTTTTCTAATACTGCAGTCTTCACGGTTCAATCGAAACGATGCACTGCAATATTAACAGTGCAAATTCCGTTTATTTTCCATCACTGAAGATGTTGTCTCTCGGCAATGATATTGTGGATCAGAACGAATTAGTTTTCTTTCTCGCTGGCTGCCCCAGGCTGGAGACTTTGCAGGTTTACGTTTACCTTTCTTCCGATGATGACATGTCTTTGACCAAAGTTCTAGTGACACCTTCCTCCAGCTCGAACAGCTTGATATCCGCTGATGACAATTTCACCTGGAGTTACTTTAATATAAATGAAGGACGTTTTAAATTGGGCATCGTTGGTAACTTCCGTAATATGGTGGAAGCATTTCTTGATGTTTTTTTCCCGGCTGAAAGTGAATTTGTAGACCCTATTCTCAACCTCATCCAAGATACCAGAAGTGAATTTCATAGAAGCCTCATAATTAACTATGAGGATGTTTATCTACAGTTGCATCATTCGATGTCAAAGGTGAAGTTTTACTTTTATGGTTTACATGTTTTAAAATGTGTAGTTTGGTTCTAATATTGCGTTGTCATCACTTGTTGTTATCGTGTATGTGCAGTGGCCAACTCATGCACCTGTTCTAAATTATCCTGAATTTCGCTATTTACATCATTTGAAGTTCATCCTTCCATGTTTCAACTCAAATCTTCTGGTCCATTGTCTTGAGCAATGCCACATGCTTCAAGTTCTCATAATTCAGAGCAACAAGGTTTGTTGAATTTTCAAATTTAATTCAAACATTCTTTGGTCGGTCTATATATACATGCATGTATGTCTCCATTTGTACACAAATATATTTTTGAGTCACGGCTTGGTGTCCCTTGTTGCTCGTAGGAGGAACAACCACCATTGAGAACATGGCAGCCGGAGTCAACAAGAGTTTCTGAATGTCTCAAGTCCCACCTGACCTATATTCACATAGAAGGATCAAGGACTTGAAGATGAGCTGACATTTGCTGAATATATCTTGCGGAACGGACTTGTTTTGGAGACAATGCTTGTTTTTGTTGATATTTCAATGGACATAACAAACAAGTACCGTTCTATCAAAAGATTAACAAATGTAACGAAGGGCTCTGTCAAATGCCAACTTAAATTTGACCCAGATGTATCTTCTTAAGGTGAAAACTCTCGGTCTCTTTTTTATCTGTCCTACATAATTCCATACCCCCAATGTATCCAAATTATATTTGGTCTATTTGTTTTCTCATAAATATGCCTAAATGAGATCCTTACTTTGTACTACATTAAATTATTATTCTAGTGTTTCATGTGTAACAATGTTTGATTTAGGATTTTGATGTTTGCAATAGTTGAAATTTGCTAAATTTATAAGGTTGAGAAAGTGATGCTCTTAACATGCTTACTGATATTGTTGGAAAAGCTTCTAGATTTTGTGCCACTTATGCCAACTTACTGA
Protein sequence

>MS.gene055883.t1

MAEESSMMKRLKAQEECDPVVDRLSSLPDSLLCHIMSFLPTKISVTTMSLVSRRYLHLWKDLQVLYFDFHHTIPFQTVSSLVNSVLVLRKSRNIQNFHLTFAFDRLQRGCVLEKLQSKNAEAWILAAIGPHLQQLYLNVDGCIDIPRSLFINCTNLVSLSLHGSIETMHCNINSANSVYFPSLKMLSLGNDIVDQNELVFFLAGCPRLETLQVYVYLSSDDDMSLTKVLVTPSSSSNSLISADDNFTWSYFNINEGRFKLGIVGNFRNMVEAFLDVFFPAESEFVDPILNLIQDTRSEFHRSLIINYEDVYLQLHHSMSKWPTHAPVLNYPEFRYLHHLKFILPCFNSNLLVHCLEQCHMLQVLIIQSNKVEKVMLLTCLLILLEKLLDFVPLMPTY