AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34804

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34804.t1 MTR_8g062300 58.553 456 134 10 1 419 1 438 5.58e-160 459
MS.gene34804.t1 MTR_8g070950 55.399 426 149 12 3 419 2 395 5.54e-134 392
MS.gene34804.t1 MTR_8g062010 57.349 415 146 13 12 419 1 391 7.78e-133 389
MS.gene34804.t1 MTR_8g062080 53.191 423 160 12 8 419 11 406 2.21e-126 373
MS.gene34804.t1 MTR_8g062310 52.618 401 153 9 20 419 25 389 1.10e-124 368
MS.gene34804.t1 MTR_8g469020 45.872 436 174 12 18 419 5 412 6.05e-111 333
MS.gene34804.t1 MTR_8g058965 47.738 442 173 15 11 419 1 417 1.31e-110 333
MS.gene34804.t1 MTR_4g050250 47.085 446 168 16 1 404 1 420 1.21e-108 327
MS.gene34804.t1 MTR_8g467790 45.614 399 150 12 20 403 148 494 2.64e-94 293
MS.gene34804.t1 MTR_8g468810 39.180 439 225 15 4 419 3 422 2.36e-66 219
MS.gene34804.t1 MTR_8g058970 37.748 453 226 15 4 419 3 436 3.86e-65 216
MS.gene34804.t1 MTR_8g062270 36.830 429 216 15 20 419 16 418 5.18e-61 204
MS.gene34804.t1 MTR_2g083840 36.239 436 231 13 5 419 7 416 6.37e-61 204
MS.gene34804.t1 MTR_8g468840 36.993 419 227 12 20 419 14 414 2.69e-56 192
MS.gene34804.t1 MTR_4g032730 33.996 453 232 14 8 419 4 430 5.35e-56 192
MS.gene34804.t1 MTR_8g468810 39.433 388 193 15 4 368 3 371 4.64e-55 187
MS.gene34804.t1 MTR_8g014260 34.086 443 237 19 1 419 1 412 3.31e-54 186
MS.gene34804.t1 MTR_8g445780 36.207 406 217 15 20 411 13 390 2.07e-52 180
MS.gene34804.t1 MTR_8g467800 34.921 441 231 15 8 419 4 417 2.39e-51 179
MS.gene34804.t1 MTR_5g092960 32.494 437 239 11 15 419 14 426 1.67e-48 172
MS.gene34804.t1 MTR_8g468760 34.483 435 201 15 12 419 1 378 6.13e-46 164
MS.gene34804.t1 MTR_8g468810 40.260 308 150 11 4 288 3 299 2.56e-45 159
MS.gene34804.t1 MTR_8g468810 40.260 308 150 11 4 288 3 299 3.72e-45 159
MS.gene34804.t1 MTR_8g468810 40.260 308 150 11 4 288 3 299 6.78e-45 159
MS.gene34804.t1 MTR_2g086020 84.536 97 15 0 322 418 179 275 1.80e-44 157
MS.gene34804.t1 MTR_2g086020 50.000 156 73 3 45 198 1 153 9.40e-37 137
MS.gene34804.t1 MTR_5g092960 32.199 382 207 10 15 368 14 371 1.86e-35 135
MS.gene34804.t1 MTR_8g467800 37.705 305 151 13 8 288 4 293 9.25e-33 126
MS.gene34804.t1 MTR_8g077460 38.710 217 116 8 20 225 10 220 2.36e-30 120
MS.gene34804.t1 MTR_8g077460 41.284 109 60 3 311 419 233 337 4.21e-17 82.8
MS.gene34804.t1 MTR_5g021550 44.242 165 74 6 5 157 4 162 1.49e-28 110
MS.gene34804.t1 MTR_8g014290 39.306 173 77 7 1 158 1 160 3.40e-25 101
MS.gene34804.t1 MTR_8g014160 31.250 288 171 10 20 287 41 321 1.06e-23 102
MS.gene34804.t1 MTR_4g024710 38.650 163 82 6 258 419 164 309 2.41e-21 94.7
MS.gene34804.t1 MTR_7g026500 26.097 433 221 14 7 417 1 356 9.88e-21 94.0
MS.gene34804.t1 MTR_3g099800 80.000 50 10 0 370 419 19 68 1.27e-20 86.7
MS.gene34804.t1 MTR_8g014210 37.267 161 83 6 260 419 50 193 1.34e-18 84.7
MS.gene34804.t1 MTR_4g024720 39.231 130 67 5 4 126 3 127 1.67e-18 84.0
MS.gene34804.t1 MTR_5g073420 32.461 191 118 6 20 205 11 195 3.00e-17 83.2
MS.gene34804.t1 MTR_7g056447 33.333 198 115 6 12 204 1 186 6.41e-17 81.3
MS.gene34804.t1 MTR_4g024745 30.112 269 139 11 170 419 25 263 6.87e-17 80.9
MS.gene34804.t1 MTR_5g084070 26.777 422 259 18 8 417 3 386 1.22e-16 82.0
MS.gene34804.t1 MTR_7g032380 35.385 195 107 7 20 207 11 193 8.41e-16 78.6
MS.gene34804.t1 MTR_5g080710 26.728 434 254 18 3 417 24 412 9.12e-16 79.3
MS.gene34804.t1 MTR_1g041245 30.918 207 122 5 18 216 9 202 1.82e-15 77.8
MS.gene34804.t1 MTR_3g107120 26.492 419 216 19 20 417 9 356 3.18e-15 77.4
MS.gene34804.t1 MTR_7g098510 25.522 431 226 19 1 417 1 350 1.20e-14 75.5
MS.gene34804.t1 MTR_4g024770 50.000 64 29 1 1 64 8 68 2.36e-14 68.2
MS.gene34804.t1 MTR_7g115270 30.435 207 121 7 20 213 12 208 2.58e-14 74.7
MS.gene34804.t1 MTR_5g007410 24.880 418 248 13 6 419 14 369 2.60e-14 74.7
MS.gene34804.t1 MTR_3g107100 30.049 203 129 9 10 208 2 195 4.90e-14 73.9
MS.gene34804.t1 MTR_2g008320 35.204 196 109 8 20 207 52 237 6.87e-14 73.6
MS.gene34804.t1 MTR_5g008950 29.524 210 133 7 22 230 1 196 9.74e-14 72.8
MS.gene34804.t1 MTR_3g036250 29.949 197 119 7 19 207 4 189 3.32e-13 70.9
MS.gene34804.t1 MTR_8g014180 55.172 58 24 1 4 61 3 58 3.75e-13 64.3
MS.gene34804.t1 MTR_2g007500 30.189 212 112 7 7 207 1 187 3.90e-13 70.5
MS.gene34804.t1 MTR_3g105060 31.980 197 122 9 20 213 14 201 4.17e-13 70.9
MS.gene34804.t1 MTR_1g492880 39.785 93 52 2 12 103 1 90 5.74e-13 65.1
MS.gene34804.t1 MTR_1g492880 39.785 93 52 2 12 103 1 90 6.55e-13 65.1
MS.gene34804.t1 MTR_7g034110 45.238 84 42 2 20 103 11 90 8.66e-13 69.7
MS.gene34804.t1 MTR_2g007400 29.384 211 135 7 17 215 9 217 8.98e-13 69.7
MS.gene34804.t1 MTR_2g007450 30.357 224 132 8 9 211 5 225 1.38e-12 69.3
MS.gene34804.t1 MTR_7g033990 30.000 210 117 7 17 212 8 201 1.59e-12 69.3
MS.gene34804.t1 MTR_3g023630 30.964 197 111 8 19 205 7 188 2.06e-12 68.6
MS.gene34804.t1 MTR_3g040700 28.866 194 125 7 19 207 42 227 2.36e-12 68.6
MS.gene34804.t1 MTR_4g098590 30.808 198 120 7 22 207 1 193 3.18e-12 68.2
MS.gene34804.t1 MTR_2g008310 33.333 192 114 6 20 207 2 183 3.47e-12 68.2
MS.gene34804.t1 MTR_7g056433 32.524 206 124 7 7 207 1 196 4.77e-12 67.4
MS.gene34804.t1 MTR_5g083890 31.884 207 110 7 20 203 6 204 1.05e-11 67.0
MS.gene34804.t1 MTR_8g085430 31.500 200 115 7 20 207 11 200 1.28e-11 65.9
MS.gene34804.t1 MTR_7g098480 30.476 210 128 6 1 205 1 197 1.64e-11 66.2
MS.gene34804.t1 MTR_7g055943 27.143 210 137 7 2 205 22 221 2.40e-11 65.5
MS.gene34804.t1 MTR_1g107385 31.441 229 135 10 3 216 20 241 4.15e-11 64.7
MS.gene34804.t1 MTR_7g034115 33.505 194 116 7 18 207 9 193 4.79e-11 64.3
MS.gene34804.t1 MTR_3g467400 29.386 228 132 9 18 223 1 221 5.72e-11 63.9
MS.gene34804.t1 MTR_7g033945 30.814 172 99 6 20 182 11 171 5.92e-11 61.6
MS.gene34804.t1 MTR_4g051480 29.936 157 100 5 20 175 25 172 7.03e-11 64.7
MS.gene34804.t1 MTR_4g092300 29.936 157 100 5 20 175 25 172 7.04e-11 64.7
MS.gene34804.t1 MTR_7g033940 33.858 127 74 5 15 139 89 207 8.25e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34804.t1 AT4G10400 26.895 409 248 14 20 417 2 370 1.12e-19 90.9
MS.gene34804.t1 AT4G10400 26.895 409 248 14 20 417 2 370 1.12e-19 90.9
MS.gene34804.t1 AT4G10400 26.895 409 248 14 20 417 2 370 1.12e-19 90.9
MS.gene34804.t1 AT4G09920 32.335 167 103 4 20 184 2 160 9.91e-16 78.2
MS.gene34804.t1 AT4G09920 32.335 167 103 4 20 184 2 160 9.91e-16 78.2
MS.gene34804.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.28e-15 77.4
MS.gene34804.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.28e-15 77.4
MS.gene34804.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.28e-15 77.4
MS.gene34804.t1 AT1G32375 27.506 429 232 19 20 417 2 382 1.31e-15 78.6
MS.gene34804.t1 AT1G32375 27.506 429 232 19 20 417 2 382 1.31e-15 78.6
MS.gene34804.t1 AT1G78750 26.196 439 277 19 4 417 2 418 1.62e-15 78.6
MS.gene34804.t1 AT1G78750 26.196 439 277 19 4 417 2 418 1.62e-15 78.6
MS.gene34804.t1 AT5G50270 28.404 426 236 18 20 417 2 386 3.16e-15 77.4
MS.gene34804.t1 AT5G56440 34.392 189 113 4 20 205 2 182 1.07e-13 72.8
MS.gene34804.t1 AT4G00160 27.913 412 252 14 20 417 16 396 5.91e-13 70.9
MS.gene34804.t1 AT3G59190 30.460 174 104 5 20 184 12 177 7.76e-13 70.5
MS.gene34804.t1 AT3G59190 30.460 174 104 5 20 184 12 177 1.01e-12 69.7
MS.gene34804.t1 AT3G59200 30.151 199 114 7 20 205 7 193 1.59e-12 68.9
MS.gene34804.t1 AT4G13985 31.841 201 116 9 19 207 18 209 2.34e-12 68.9
MS.gene34804.t1 AT1G16930 29.126 206 127 7 8 207 3 195 2.88e-12 68.6
MS.gene34804.t1 AT1G16930 29.126 206 127 7 8 207 3 195 2.88e-12 68.6
MS.gene34804.t1 AT1G16930 29.126 206 127 7 8 207 3 195 2.88e-12 68.6
MS.gene34804.t1 AT3G59200 30.151 199 114 7 20 205 7 193 3.69e-12 68.6
MS.gene34804.t1 AT4G14103 31.282 195 115 5 20 205 8 192 4.16e-12 68.2
MS.gene34804.t1 AT1G48400 30.052 193 121 4 20 205 10 195 4.76e-12 68.2
MS.gene34804.t1 AT4G14103 31.088 193 118 4 20 205 8 192 5.09e-12 67.4
MS.gene34804.t1 AT4G14103 31.088 193 118 4 20 205 8 192 5.09e-12 67.4
MS.gene34804.t1 AT1G48400 30.052 193 121 4 20 205 10 195 5.18e-12 67.8
MS.gene34804.t1 AT3G52680 32.143 196 114 6 20 208 21 204 1.00e-11 67.0
MS.gene34804.t1 AT5G53635 24.700 417 270 12 22 417 2 395 1.22e-11 66.6
MS.gene34804.t1 AT4G13965 26.603 421 239 15 19 416 9 382 1.32e-11 66.2
MS.gene34804.t1 AT5G53840 24.885 434 237 17 20 417 18 398 2.01e-11 65.9
MS.gene34804.t1 AT3G52680 32.143 196 114 6 20 208 21 204 2.05e-11 65.5

Find 105 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATAGTTTACCTTCTATT+TGG 0.229961 8.3:+40056988 MS.gene34804:CDS
CATTGGCAACTTTCACAGTA+TGG 0.259899 8.3:+40057188 MS.gene34804:CDS
AAAATTGTCATTGGCCGATT+TGG 0.299413 8.3:-40057109 None:intergenic
CTCGCCGGTGGCGCCACCTC+TGG 0.341008 8.3:+40056464 MS.gene34804:CDS
TGATGTAAACGGCTAAATTC+AGG 0.379198 8.3:-40057484 None:intergenic
GGAAGGTGGAATTAAAGCTT+TGG 0.380976 8.3:-40057070 None:intergenic
TGCATCAGATTGGGCATCAT+TGG 0.387142 8.3:+40057171 MS.gene34804:CDS
CTCGGATGAGGTTGAGAATA+GGG 0.388430 8.3:-40057260 None:intergenic
CTGACTTATGTTCACATAGA+AGG 0.406124 8.3:+40057798 MS.gene34804:CDS
GTAAAGGAGGTTGGAGCAAT+TGG 0.413248 8.3:-40056764 None:intergenic
TATGAATGTCGCGGAATTTG+CGG 0.415114 8.3:-40056579 None:intergenic
TAGATTCACATTCGTTCAAA+AGG 0.416585 8.3:+40056524 MS.gene34804:CDS
AAATAATGAATGCATCAGAT+TGG 0.421459 8.3:+40057161 MS.gene34804:CDS
GAGATAGAGTTGTTGAAGGT+GGG 0.425273 8.3:-40056695 None:intergenic
ACTTGGGACTGCCAAATAGA+AGG 0.427636 8.3:-40056999 None:intergenic
CGATTTGGAGCTCTCGGAGC+TGG 0.442706 8.3:-40057094 None:intergenic
CCTTCCAGAGGTGGCGCCAC+CGG 0.463852 8.3:-40056468 None:intergenic
AATAATGAATGCATCAGATT+GGG 0.466409 8.3:+40057162 MS.gene34804:CDS
AGTCCAAGTAAAATTGTCAT+TGG 0.468154 8.3:-40057118 None:intergenic
CATCATAGCGACGGAGGTTG+TGG 0.470605 8.3:-40056434 None:intergenic
AACCCGCAAAACTTCCAACT+TGG 0.473471 8.3:-40057016 None:intergenic
CGCGGAATTTGCGGAGGGCG+AGG 0.475468 8.3:-40056570 None:intergenic
GCTCCAACCTCCTTTACCTC+AGG 0.478577 8.3:+40056770 MS.gene34804:CDS
TATACATCATAGCAGTCAGC+TGG 0.478695 8.3:-40057315 None:intergenic
TCTCGGATGAGGTTGAGAAT+AGG 0.481952 8.3:-40057261 None:intergenic
GAAGGTGGAATTAAAGCTTT+GGG 0.484659 8.3:-40057069 None:intergenic
GCAATTATAACCTGAGGTAA+AGG 0.484740 8.3:-40056780 None:intergenic
TGTAGGTTCTTATAGTATAG+AGG 0.487842 8.3:+40056894 MS.gene34804:CDS
CTCAACCTCATCCGAGATGA+TGG 0.488519 8.3:+40057267 MS.gene34804:CDS
TACTCGGCTTTAATCGATCC+GGG 0.489291 8.3:+40056614 MS.gene34804:CDS
ACGTGGATCCTTGCTGCCAT+TGG 0.491503 8.3:+40056670 MS.gene34804:CDS
ATGTTTCGACTCAAATCTGC+TGG 0.494251 8.3:+40057525 MS.gene34804:CDS
CATAAGTTCCATCTCATACT+CGG 0.496407 8.3:+40056598 MS.gene34804:CDS
CTTCTATGTGAACATAAGTC+AGG 0.496958 8.3:-40057797 None:intergenic
ATACTCGGCTTTAATCGATC+CGG 0.499361 8.3:+40056613 MS.gene34804:CDS
GCCATTGAGAACATGGCAGC+TGG 0.499987 8.3:+40057740 MS.gene34804:CDS
ATTGGCAACTTTCACAGTAT+GGG 0.502787 8.3:+40057189 MS.gene34804:CDS
TGAACTTCAGATGATGTAAA+CGG 0.503521 8.3:-40057495 None:intergenic
GACAAATTCACTTTCAGTCA+AGG 0.506419 8.3:-40057235 None:intergenic
CAATTGGTCAAGAGGGAAGG+AGG 0.511905 8.3:-40056748 None:intergenic
TGCCATGTTCTCAATGGCAG+TGG 0.512316 8.3:-40057735 None:intergenic
TTCTGCCATCATCTCGGATG+AGG 0.513011 8.3:-40057272 None:intergenic
ATTGGCCGATTTGGAGCTCT+CGG 0.514913 8.3:-40057100 None:intergenic
TTGTTGAAGGTGGGGTCCAA+TGG 0.515637 8.3:-40056686 None:intergenic
TCCAGCTGCCATGTTCTCAA+TGG 0.515812 8.3:-40057741 None:intergenic
GTTCACATAGAAGGATATCA+AGG 0.522561 8.3:+40057807 MS.gene34804:CDS
TAACCTGAGGTAAAGGAGGT+TGG 0.524882 8.3:-40056773 None:intergenic
TGTAGAGATAGAGTTGTTGA+AGG 0.526137 8.3:-40056699 None:intergenic
CAGCTCCGAGAGCTCCAAAT+CGG 0.527982 8.3:+40057095 MS.gene34804:CDS
GAGCTCTCGGAGCTGGAGGA+AGG 0.528347 8.3:-40057087 None:intergenic
AGAGATAGAGTTGTTGAAGG+TGG 0.529187 8.3:-40056696 None:intergenic
TTGGCAACTTTCACAGTATG+GGG 0.536652 8.3:+40057190 MS.gene34804:CDS
ATGAAAGTTGTCATAAATCC+CGG 0.539720 8.3:-40056632 None:intergenic
GGAGGTTGTGGTAGGGAGGA+AGG 0.546045 8.3:-40056422 None:intergenic
CCGGTGGCGCCACCTCTGGA+AGG 0.547084 8.3:+40056468 MS.gene34804:CDS
TCTATTTGGCAGTCCCAAGT+TGG 0.550189 8.3:+40057002 MS.gene34804:CDS
AGCAGATTTGAGTCGAAACA+TGG 0.553943 8.3:-40057523 None:intergenic
TGTGGCATTGCTCAAGAACA+TGG 0.554101 8.3:-40057549 None:intergenic
CTCTCGGAGCTGGAGGAAGG+TGG 0.556854 8.3:-40057084 None:intergenic
AACCACTGCCATTGAGAACA+TGG 0.558537 8.3:+40057733 MS.gene34804:CDS
ATATGACACAGGATCGAGTC+CGG 0.559164 8.3:-40056394 None:intergenic
AACCTACTCGACGATGGCAA+CGG 0.559988 8.3:+40056306 None:intergenic
TTCAGGATAATTTAGAACAC+GGG 0.564152 8.3:-40057467 None:intergenic
AATTGGTCAAGAGGGAAGGA+GGG 0.570036 8.3:-40056747 None:intergenic
ACCCGCAAAACTTCCAACTT+GGG 0.570628 8.3:-40057015 None:intergenic
GGACAGATTGAGCAGTCTAC+CGG 0.570908 8.3:+40056375 MS.gene34804:CDS
ATAGCGACGGAGGTTGTGGT+AGG 0.574834 8.3:-40056430 None:intergenic
TAATTTAGAACACGGGCATG+AGG 0.579387 8.3:-40057460 None:intergenic
AGATAGAGTTGTTGAAGGTG+GGG 0.580102 8.3:-40056694 None:intergenic
GATTAAAGCCGAGTATGAGA+TGG 0.586475 8.3:-40056606 None:intergenic
AGAGACAGCACTTTGAGCAA+TGG 0.588422 8.3:-40056940 None:intergenic
ATTCAGTTCTGCCATCATCT+CGG 0.593799 8.3:-40057278 None:intergenic
GAGCAATTGGTCAAGAGGGA+AGG 0.596231 8.3:-40056751 None:intergenic
GGTTGGAGCAATTGGTCAAG+AGG 0.598188 8.3:-40056756 None:intergenic
ATCTCGCTTGTATTCGTACG+TGG 0.599453 8.3:+40056653 MS.gene34804:CDS
GTTGCGTCATTCAACATCAA+AGG 0.603041 8.3:+40057344 MS.gene34804:CDS
CTATGTGAACATAAGTCAGG+TGG 0.604289 8.3:-40057794 None:intergenic
GAATGTCGCGGAATTTGCGG+AGG 0.613849 8.3:-40056576 None:intergenic
GATGGCAACGGAGTCAAGCA+TGG 0.614265 8.3:+40056318 None:intergenic
TAGCGACGGAGGTTGTGGTA+GGG 0.614525 8.3:-40056429 None:intergenic
GTTGGAGCAATTGGTCAAGA+GGG 0.618704 8.3:-40056755 None:intergenic
CGACGGAGGTTGTGGTAGGG+AGG 0.621858 8.3:-40056426 None:intergenic
GGTGGGGTCCAATGGCAGCA+AGG 0.624675 8.3:-40056678 None:intergenic
ATTATAACCTGAGGTAAAGG+AGG 0.629004 8.3:-40056777 None:intergenic
ATTCAGGATAATTTAGAACA+CGG 0.629666 8.3:-40057468 None:intergenic
TGATGAGAGTCTCTCGCCGG+TGG 0.630142 8.3:+40056452 MS.gene34804:CDS
AATTTAGAACACGGGCATGA+GGG 0.631219 8.3:-40057459 None:intergenic
TTTGAGCAATGGAAAATGAA+CGG 0.634422 8.3:-40056929 None:intergenic
TTTGGAGCTCTCGGAGCTGG+AGG 0.636682 8.3:-40057091 None:intergenic
TCTGCAGATCCTTCCAGAGG+TGG 0.638103 8.3:-40056477 None:intergenic
TATGTGAACATAAGTCAGGT+GGG 0.640734 8.3:-40057793 None:intergenic
CTATGATGAGAGTCTCTCGC+CGG 0.640993 8.3:+40056449 MS.gene34804:CDS
ATACATCATAGCAGTCAGCT+GGG 0.647711 8.3:-40057314 None:intergenic
TCTCATAATTCAGAGCAACA+AGG 0.652411 8.3:+40057582 MS.gene34804:CDS
GGAAGGACATGATATGACAC+AGG 0.667460 8.3:-40056405 None:intergenic
AGAGACTCTCATCATAGCGA+CGG 0.669339 8.3:-40056443 None:intergenic
TGGCAACTTTCACAGTATGG+GGG 0.681502 8.3:+40057191 MS.gene34804:CDS
AATGTCGCGGAATTTGCGGA+GGG 0.681823 8.3:-40056575 None:intergenic
GATGGAACTTATGAATGTCG+CGG 0.686296 8.3:-40056588 None:intergenic
GACTCTCATCATAGCGACGG+AGG 0.689212 8.3:-40056440 None:intergenic
TTATGAGAACTTGAAGCATG+TGG 0.695487 8.3:-40057567 None:intergenic
TGCAGAAGAAGACAACGTCA+CGG 0.699654 8.3:+40056354 MS.gene34804:CDS
ACTGCTATGATGTATATCTG+CGG 0.701238 8.3:+40057322 MS.gene34804:CDS
ATATCTGCAGATCCTTCCAG+AGG 0.715901 8.3:-40056480 None:intergenic
CATCATAGCAGTCAGCTGGG+AGG 0.736987 8.3:-40057311 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATACATACATGTATATAT+AGG - chr8.3:40057650-40057669 None:intergenic 20.0%
!! AGTTTAATACAAACAATCTT+TGG + chr8.3:40057620-40057639 MS.gene34804:intron 20.0%
!! ATGGTTTATATGTATATCTA+CGG + chr8.3:40057382-40057401 MS.gene34804:intron 20.0%
!! GTCATTACTCAAAAATATAT+TGG - chr8.3:40057686-40057705 None:intergenic 20.0%
!!! TATATTTTTGAGTAATGACA+TGG + chr8.3:40057687-40057706 MS.gene34804:intron 20.0%
! AAATAATGAATGCATCAGAT+TGG + chr8.3:40057161-40057180 MS.gene34804:CDS 25.0%
! AATAATGAATGCATCAGATT+GGG + chr8.3:40057162-40057181 MS.gene34804:CDS 25.0%
! GAAATAGTTTACCTTCTATT+TGG + chr8.3:40056988-40057007 MS.gene34804:CDS 25.0%
! TAAAAAGCAATTATAACCTG+AGG - chr8.3:40056789-40056808 None:intergenic 25.0%
! TTATATGTATATCTACGGTT+TGG + chr8.3:40057387-40057406 MS.gene34804:intron 25.0%
!! ATTCAGGATAATTTAGAACA+CGG - chr8.3:40057471-40057490 None:intergenic 25.0%
!!! AAGGTGATGTTTTATTCTTA+TGG + chr8.3:40057363-40057382 MS.gene34804:intron 25.0%
!!! CATTTGCTGAATATATTTTG+CGG + chr8.3:40057847-40057866 MS.gene34804:CDS 25.0%
AGTCCAAGTAAAATTGTCAT+TGG - chr8.3:40057121-40057140 None:intergenic 30.0%
ATGAAAGTTGTCATAAATCC+CGG - chr8.3:40056635-40056654 None:intergenic 30.0%
GAAATGTTAGAAGTGAGTTT+GGG - chr8.3:40056814-40056833 None:intergenic 30.0%
TAGATTCACATTCGTTCAAA+AGG + chr8.3:40056524-40056543 MS.gene34804:CDS 30.0%
TGAAATGTTAGAAGTGAGTT+TGG - chr8.3:40056815-40056834 None:intergenic 30.0%
TGAACTTCAGATGATGTAAA+CGG - chr8.3:40057498-40057517 None:intergenic 30.0%
TGTAGGTTCTTATAGTATAG+AGG + chr8.3:40056894-40056913 MS.gene34804:CDS 30.0%
TTTGAGCAATGGAAAATGAA+CGG - chr8.3:40056932-40056951 None:intergenic 30.0%
! TTCAGGATAATTTAGAACAC+GGG - chr8.3:40057470-40057489 None:intergenic 30.0%
! TTTTAGACTCACAATTTCTG+CGG - chr8.3:40056968-40056987 None:intergenic 30.0%
!! TCTTTTGAAAACAGTCTTGT+AGG + chr8.3:40056877-40056896 MS.gene34804:intron 30.0%
AAAATTGTCATTGGCCGATT+TGG - chr8.3:40057112-40057131 None:intergenic 35.0%
ACTGCTATGATGTATATCTG+CGG + chr8.3:40057322-40057341 MS.gene34804:CDS 35.0%
ATTATAACCTGAGGTAAAGG+AGG - chr8.3:40056780-40056799 None:intergenic 35.0%
ATTGGCAACTTTCACAGTAT+GGG + chr8.3:40057189-40057208 MS.gene34804:CDS 35.0%
CATAAGTTCCATCTCATACT+CGG + chr8.3:40056598-40056617 MS.gene34804:CDS 35.0%
CTGACTTATGTTCACATAGA+AGG + chr8.3:40057798-40057817 MS.gene34804:CDS 35.0%
CTTCTATGTGAACATAAGTC+AGG - chr8.3:40057800-40057819 None:intergenic 35.0%
GAAGGTGGAATTAAAGCTTT+GGG - chr8.3:40057072-40057091 None:intergenic 35.0%
GACAAATTCACTTTCAGTCA+AGG - chr8.3:40057238-40057257 None:intergenic 35.0%
GCAATTATAACCTGAGGTAA+AGG - chr8.3:40056783-40056802 None:intergenic 35.0%
GTTCACATAGAAGGATATCA+AGG + chr8.3:40057807-40057826 MS.gene34804:CDS 35.0%
TATGTGAACATAAGTCAGGT+GGG - chr8.3:40057796-40057815 None:intergenic 35.0%
TCTCATAATTCAGAGCAACA+AGG + chr8.3:40057582-40057601 MS.gene34804:CDS 35.0%
TGATGTAAACGGCTAAATTC+AGG - chr8.3:40057487-40057506 None:intergenic 35.0%
TGTAGAGATAGAGTTGTTGA+AGG - chr8.3:40056702-40056721 None:intergenic 35.0%
TTATGAGAACTTGAAGCATG+TGG - chr8.3:40057570-40057589 None:intergenic 35.0%
! GCTGAATATATTTTGCGGAA+TGG + chr8.3:40057852-40057871 MS.gene34804:CDS 35.0%
AATGACATGGTTTCCCTTGT+TGG + chr8.3:40057700-40057719 MS.gene34804:intron 40.0%
AGAGATAGAGTTGTTGAAGG+TGG - chr8.3:40056699-40056718 None:intergenic 40.0%
AGATAGAGTTGTTGAAGGTG+GGG - chr8.3:40056697-40056716 None:intergenic 40.0%
AGCAGATTTGAGTCGAAACA+TGG - chr8.3:40057526-40057545 None:intergenic 40.0%
ATACATCATAGCAGTCAGCT+GGG - chr8.3:40057317-40057336 None:intergenic 40.0%
ATGTTTCGACTCAAATCTGC+TGG + chr8.3:40057525-40057544 MS.gene34804:CDS 40.0%
ATTCAGTTCTGCCATCATCT+CGG - chr8.3:40057281-40057300 None:intergenic 40.0%
CATTGGCAACTTTCACAGTA+TGG + chr8.3:40057188-40057207 MS.gene34804:CDS 40.0%
CTATGTGAACATAAGTCAGG+TGG - chr8.3:40057797-40057816 None:intergenic 40.0%
GAGATAGAGTTGTTGAAGGT+GGG - chr8.3:40056698-40056717 None:intergenic 40.0%
GATGGAACTTATGAATGTCG+CGG - chr8.3:40056591-40056610 None:intergenic 40.0%
GATTAAAGCCGAGTATGAGA+TGG - chr8.3:40056609-40056628 None:intergenic 40.0%
GGAAGGTGGAATTAAAGCTT+TGG - chr8.3:40057073-40057092 None:intergenic 40.0%
GTTGCGTCATTCAACATCAA+AGG + chr8.3:40057344-40057363 MS.gene34804:CDS 40.0%
TATACATCATAGCAGTCAGC+TGG - chr8.3:40057318-40057337 None:intergenic 40.0%
TATGAATGTCGCGGAATTTG+CGG - chr8.3:40056582-40056601 None:intergenic 40.0%
TTGGCAACTTTCACAGTATG+GGG + chr8.3:40057190-40057209 MS.gene34804:CDS 40.0%
! AATTTAGAACACGGGCATGA+GGG - chr8.3:40057462-40057481 None:intergenic 40.0%
! ATACTCGGCTTTAATCGATC+CGG + chr8.3:40056613-40056632 MS.gene34804:CDS 40.0%
! CGGCCAATGACAATTTTACT+TGG + chr8.3:40057115-40057134 MS.gene34804:CDS 40.0%
! TAATTTAGAACACGGGCATG+AGG - chr8.3:40057463-40057482 None:intergenic 40.0%
AACCACTGCCATTGAGAACA+TGG + chr8.3:40057733-40057752 MS.gene34804:CDS 45.0%
AACCCGCAAAACTTCCAACT+TGG - chr8.3:40057019-40057038 None:intergenic 45.0%
AATTGGTCAAGAGGGAAGGA+GGG - chr8.3:40056750-40056769 None:intergenic 45.0%
ACCCGCAAAACTTCCAACTT+GGG - chr8.3:40057018-40057037 None:intergenic 45.0%
ACTTGGGACTGCCAAATAGA+AGG - chr8.3:40057002-40057021 None:intergenic 45.0%
AGAGACAGCACTTTGAGCAA+TGG - chr8.3:40056943-40056962 None:intergenic 45.0%
AGAGACTCTCATCATAGCGA+CGG - chr8.3:40056446-40056465 None:intergenic 45.0%
ATATCTGCAGATCCTTCCAG+AGG - chr8.3:40056483-40056502 None:intergenic 45.0%
ATATGACACAGGATCGAGTC+CGG - chr8.3:40056397-40056416 None:intergenic 45.0%
ATCTCGCTTGTATTCGTACG+TGG + chr8.3:40056653-40056672 MS.gene34804:CDS 45.0%
CTCGGATGAGGTTGAGAATA+GGG - chr8.3:40057263-40057282 None:intergenic 45.0%
GGAAGGACATGATATGACAC+AGG - chr8.3:40056408-40056427 None:intergenic 45.0%
GTAAAGGAGGTTGGAGCAAT+TGG - chr8.3:40056767-40056786 None:intergenic 45.0%
GTTGGAGCAATTGGTCAAGA+GGG - chr8.3:40056758-40056777 None:intergenic 45.0%
TAACCTGAGGTAAAGGAGGT+TGG - chr8.3:40056776-40056795 None:intergenic 45.0%
TCTATTTGGCAGTCCCAAGT+TGG + chr8.3:40057002-40057021 MS.gene34804:CDS 45.0%
TCTCGGATGAGGTTGAGAAT+AGG - chr8.3:40057264-40057283 None:intergenic 45.0%
TGCAGAAGAAGACAACGTCA+CGG + chr8.3:40056354-40056373 MS.gene34804:CDS 45.0%
TGCATCAGATTGGGCATCAT+TGG + chr8.3:40057171-40057190 MS.gene34804:CDS 45.0%
TGGCAACTTTCACAGTATGG+GGG + chr8.3:40057191-40057210 MS.gene34804:CDS 45.0%
TGTGGCATTGCTCAAGAACA+TGG - chr8.3:40057552-40057571 None:intergenic 45.0%
TTGTTATCGTGTACCTGCAG+TGG + chr8.3:40057433-40057452 MS.gene34804:intron 45.0%
! TACTCGGCTTTAATCGATCC+GGG + chr8.3:40056614-40056633 MS.gene34804:CDS 45.0%
!! GTCCCAAGTTGGAAGTTTTG+CGG + chr8.3:40057013-40057032 MS.gene34804:CDS 45.0%
!! TCCCAAGTTGGAAGTTTTGC+GGG + chr8.3:40057014-40057033 MS.gene34804:CDS 45.0%
AATGTCGCGGAATTTGCGGA+GGG - chr8.3:40056578-40056597 None:intergenic 50.0%
CAATTGGTCAAGAGGGAAGG+AGG - chr8.3:40056751-40056770 None:intergenic 50.0%
CTATGATGAGAGTCTCTCGC+CGG + chr8.3:40056449-40056468 MS.gene34804:CDS 50.0%
CTCAACCTCATCCGAGATGA+TGG + chr8.3:40057267-40057286 MS.gene34804:CDS 50.0%
GACATGGTTTCCCTTGTTGG+TGG + chr8.3:40057703-40057722 MS.gene34804:intron 50.0%
GAGCAATTGGTCAAGAGGGA+AGG - chr8.3:40056754-40056773 None:intergenic 50.0%
GGACAGATTGAGCAGTCTAC+CGG + chr8.3:40056375-40056394 MS.gene34804:CDS 50.0%
GGTTGGAGCAATTGGTCAAG+AGG - chr8.3:40056759-40056778 None:intergenic 50.0%
GTTCCTCCTACCACCAACAA+GGG - chr8.3:40057716-40057735 None:intergenic 50.0%
TCCAGCTGCCATGTTCTCAA+TGG - chr8.3:40057744-40057763 None:intergenic 50.0%
TGCCATGTTCTCAATGGCAG+TGG - chr8.3:40057738-40057757 None:intergenic 50.0%
TTCTGCCATCATCTCGGATG+AGG - chr8.3:40057275-40057294 None:intergenic 50.0%
TTGTTGAAGGTGGGGTCCAA+TGG - chr8.3:40056689-40056708 None:intergenic 50.0%
!! ATTGGCCGATTTGGAGCTCT+CGG - chr8.3:40057103-40057122 None:intergenic 50.0%
!! TGGTTTCCCTTGTTGGTGGT+AGG + chr8.3:40057707-40057726 MS.gene34804:intron 50.0%
!! TTTCCCTTGTTGGTGGTAGG+AGG + chr8.3:40057710-40057729 MS.gene34804:intron 50.0%
ACGTGGATCCTTGCTGCCAT+TGG + chr8.3:40056670-40056689 MS.gene34804:CDS 55.0%
ATAGCGACGGAGGTTGTGGT+AGG - chr8.3:40056433-40056452 None:intergenic 55.0%
CAGCTCCGAGAGCTCCAAAT+CGG + chr8.3:40057095-40057114 MS.gene34804:CDS 55.0%
CATCATAGCAGTCAGCTGGG+AGG - chr8.3:40057314-40057333 None:intergenic 55.0%
CATCATAGCGACGGAGGTTG+TGG - chr8.3:40056437-40056456 None:intergenic 55.0%
GAATGTCGCGGAATTTGCGG+AGG - chr8.3:40056579-40056598 None:intergenic 55.0%
GACTCTCATCATAGCGACGG+AGG - chr8.3:40056443-40056462 None:intergenic 55.0%
GCCATTGAGAACATGGCAGC+TGG + chr8.3:40057740-40057759 MS.gene34804:CDS 55.0%
GCTCCAACCTCCTTTACCTC+AGG + chr8.3:40056770-40056789 MS.gene34804:CDS 55.0%
GGTTCCTCCTACCACCAACA+AGG - chr8.3:40057717-40057736 None:intergenic 55.0%
TAGCGACGGAGGTTGTGGTA+GGG - chr8.3:40056432-40056451 None:intergenic 55.0%
TCTGCAGATCCTTCCAGAGG+TGG - chr8.3:40056480-40056499 None:intergenic 55.0%
GGAGGTTGTGGTAGGGAGGA+AGG - chr8.3:40056425-40056444 None:intergenic 60.0%
TGATGAGAGTCTCTCGCCGG+TGG + chr8.3:40056452-40056471 MS.gene34804:CDS 60.0%
! CGATTTGGAGCTCTCGGAGC+TGG - chr8.3:40057097-40057116 None:intergenic 60.0%
! TTTGGAGCTCTCGGAGCTGG+AGG - chr8.3:40057094-40057113 None:intergenic 60.0%
CGACGGAGGTTGTGGTAGGG+AGG - chr8.3:40056429-40056448 None:intergenic 65.0%
CTCTCGGAGCTGGAGGAAGG+TGG - chr8.3:40057087-40057106 None:intergenic 65.0%
GAGCTCTCGGAGCTGGAGGA+AGG - chr8.3:40057090-40057109 None:intergenic 65.0%
GGTGGGGTCCAATGGCAGCA+AGG - chr8.3:40056681-40056700 None:intergenic 65.0%
CCTTCCAGAGGTGGCGCCAC+CGG - chr8.3:40056471-40056490 None:intergenic 70.0%
CGCGGAATTTGCGGAGGGCG+AGG - chr8.3:40056573-40056592 None:intergenic 70.0%
GGCATGAGGGTCGCCACTGC+AGG - chr8.3:40057449-40057468 None:intergenic 70.0%
CCGGTGGCGCCACCTCTGGA+AGG + chr8.3:40056468-40056487 MS.gene34804:CDS 75.0%
! CTCGCCGGTGGCGCCACCTC+TGG + chr8.3:40056464-40056483 MS.gene34804:CDS 80.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 40056319 40057884 40056319 ID=MS.gene34804
chr8.3 mRNA 40056319 40057884 40056319 ID=MS.gene34804.t1;Parent=MS.gene34804
chr8.3 exon 40056319 40056791 40056319 ID=MS.gene34804.t1.exon1;Parent=MS.gene34804.t1
chr8.3 CDS 40056319 40056791 40056319 ID=cds.MS.gene34804.t1;Parent=MS.gene34804.t1
chr8.3 exon 40056891 40057370 40056891 ID=MS.gene34804.t1.exon2;Parent=MS.gene34804.t1
chr8.3 CDS 40056891 40057370 40056891 ID=cds.MS.gene34804.t1;Parent=MS.gene34804.t1
chr8.3 exon 40057453 40057603 40057453 ID=MS.gene34804.t1.exon3;Parent=MS.gene34804.t1
chr8.3 CDS 40057453 40057603 40057453 ID=cds.MS.gene34804.t1;Parent=MS.gene34804.t1
chr8.3 exon 40057729 40057884 40057729 ID=MS.gene34804.t1.exon4;Parent=MS.gene34804.t1
chr8.3 CDS 40057729 40057884 40057729 ID=cds.MS.gene34804.t1;Parent=MS.gene34804.t1
Gene Sequence

>MS.gene34804

ATGGCAACGGAGTCAAGCATGGAACGATCGCTTGTTGCAGAAGAAGACAACGTCACGGACAGATTGAGCAGTCTACCGGACTCGATCCTGTGTCATATCATGTCCTTCCTCCCTACCACAACCTCCGTCGCTATGATGAGAGTCTCTCGCCGGTGGCGCCACCTCTGGAAGGATCTGCAGATATTCAACTTCTGCAGTCGCAATCTAGATTCACATTCGTTCAAAAGGTTTTTATTATTCGTGAACGCTGTCCTCGCCCTCCGCAAATTCCGCGACATTCATAAGTTCCATCTCATACTCGGCTTTAATCGATCCGGGATTTATGACAACTTTCATCTCGCTTGTATTCGTACGTGGATCCTTGCTGCCATTGGACCCCACCTTCAACAACTCTATCTCTACATTGTCAATGCTGCTGTTGTAGTTCTCCCTCCTTCCCTCTTGACCAATTGCTCCAACCTCCTTTACCTCAGGTTATAATTGCTTTTTATGCCCAAACTCACTTCTAACATTTCATTTCTTGTATTTTTACTTTTTTTTTTCTTTCTAACATGTTTGTCTTTTGAAAACAGTCTTGTAGGTTCTTATAGTATAGAGGTAAAACAGTCTTCCGTTCATTTTCCATTGCTCAAAGTGCTGTCTCTCGCCGCAGAAATTGTGAGTCTAAAAGAAATAGTTTACCTTCTATTTGGCAGTCCCAAGTTGGAAGTTTTGCGGGTTTACTTTTACTTTATCTCTGAAGAACTTGTGCCCAAAGCTTTAATTCCACCTTCCTCCAGCTCCGAGAGCTCCAAATCGGCCAATGACAATTTTACTTGGACTTACTTTAAAATAGTTAACACAAATAATGAATGCATCAGATTGGGCATCATTGGCAACTTTCACAGTATGGGGGAAGCATTTCTTGATGTCTTTTCCTTGACTGAAAGTGAATTTGTCGACCCTATTCTCAACCTCATCCGAGATGATGGCAGAACTGAATTTTATAAAAACCTCCCAGCTGACTGCTATGATGTATATCTGCGGTTGCGTCATTCAACATCAAAGGTGATGTTTTATTCTTATGGTTTATATGTATATCTACGGTTTGGTACTAATAGTGCGTCATCACTTGTTGTTATCGTGTACCTGCAGTGGCGACCCTCATGCCCGTGTTCTAAATTATCCTGAATTTAGCCGTTTACATCATCTGAAGTTCATTCTTCCATGTTTCGACTCAAATCTGCTGGTCCATGTTCTTGAGCAATGCCACATGCTTCAAGTTCTCATAATTCAGAGCAACAAGGTTCGTTGACTTCTCAAGTTTAATACAAACAATCTTTGGTCTGCCTATATATACATGTATGTATGTATCTATCTGTACACCAATATATTTTTGAGTAATGACATGGTTTCCCTTGTTGGTGGTAGGAGGAACCACTGCCATTGAGAACATGGCAGCTGGAGTCAACAACAGTTTTTGAATGTCTAAAATCCCACCTGACTTATGTTCACATAGAAGGATATCAAGGACTTGAAGATGAGTTCACATTTGCTGAATATATTTTGCGGAATGGACTTCTTTGA
Protein sequence

>MS.gene34804.t1

MATESSMERSLVAEEDNVTDRLSSLPDSILCHIMSFLPTTTSVAMMRVSRRWRHLWKDLQIFNFCSRNLDSHSFKRFLLFVNAVLALRKFRDIHKFHLILGFNRSGIYDNFHLACIRTWILAAIGPHLQQLYLYIVNAAVVVLPPSLLTNCSNLLYLSLVGSYSIEVKQSSVHFPLLKVLSLAAEIVSLKEIVYLLFGSPKLEVLRVYFYFISEELVPKALIPPSSSSESSKSANDNFTWTYFKIVNTNNECIRLGIIGNFHSMGEAFLDVFSLTESEFVDPILNLIRDDGRTEFYKNLPADCYDVYLRLRHSTSKVIGDPHARVLNYPEFSRLHHLKFILPCFDSNLLVHVLEQCHMLQVLIIQSNKEEPLPLRTWQLESTTVFECLKSHLTYVHIEGYQGLEDEFTFAEYILRNGLL