AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63221

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63221.t1 MTR_8g468810 91.566 415 33 1 1 415 51 463 0.0 768
MS.gene63221.t1 MTR_8g468810 92.260 323 23 1 1 323 51 371 0.0 597
MS.gene63221.t1 MTR_8g058970 64.747 434 133 3 1 415 45 477 0.0 527
MS.gene63221.t1 MTR_8g468810 92.105 266 19 1 1 266 51 314 2.00e-173 487
MS.gene63221.t1 MTR_8g468810 92.105 266 19 1 1 266 51 314 2.88e-173 487
MS.gene63221.t1 MTR_8g468810 92.105 266 19 1 1 266 51 314 5.17e-173 488
MS.gene63221.t1 MTR_2g083840 55.131 419 176 8 1 415 47 457 1.64e-141 411
MS.gene63221.t1 MTR_8g468840 49.289 422 191 9 2 413 40 448 1.08e-119 355
MS.gene63221.t1 MTR_8g062270 48.252 429 191 11 1 414 41 453 2.75e-119 355
MS.gene63221.t1 MTR_8g467800 49.649 427 183 10 2 415 46 453 5.34e-118 352
MS.gene63221.t1 MTR_4g032730 47.964 442 182 10 3 414 43 466 1.17e-117 351
MS.gene63221.t1 MTR_5g092960 49.415 427 189 10 3 414 47 461 9.02e-115 343
MS.gene63221.t1 MTR_8g468760 48.478 427 165 9 1 414 34 418 1.03e-111 334
MS.gene63221.t1 MTR_8g014260 42.417 422 219 15 4 415 44 451 4.16e-90 280
MS.gene63221.t1 MTR_5g092960 49.096 332 151 8 3 323 47 371 4.10e-83 260
MS.gene63221.t1 MTR_8g469020 38.568 433 232 12 2 415 34 451 2.39e-80 255
MS.gene63221.t1 MTR_8g058965 41.067 431 223 12 2 415 40 456 2.51e-80 255
MS.gene63221.t1 MTR_8g445780 42.779 367 187 11 4 365 41 389 2.59e-76 242
MS.gene63221.t1 MTR_8g467800 54.373 263 108 5 2 259 46 301 6.33e-73 232
MS.gene63221.t1 MTR_8g070950 39.716 423 218 14 1 415 41 434 1.53e-72 234
MS.gene63221.t1 MTR_4g050250 40.945 381 192 11 1 357 47 418 2.63e-66 218
MS.gene63221.t1 MTR_8g062010 37.292 421 233 13 1 415 35 430 7.73e-63 209
MS.gene63221.t1 MTR_8g062080 37.089 426 228 16 1 415 49 445 2.44e-61 205
MS.gene63221.t1 MTR_8g062310 35.749 414 225 13 4 415 54 428 3.83e-60 201
MS.gene63221.t1 MTR_8g062300 34.899 447 238 13 1 415 52 477 1.21e-55 191
MS.gene63221.t1 MTR_4g024710 41.776 304 158 11 117 415 58 347 5.29e-54 183
MS.gene63221.t1 MTR_8g014210 45.267 243 120 9 174 415 1 231 8.27e-47 160
MS.gene63221.t1 MTR_8g014160 38.267 277 147 8 2 263 67 334 1.77e-44 160
MS.gene63221.t1 MTR_8g014160 48.718 78 37 1 338 415 351 425 5.91e-14 73.6
MS.gene63221.t1 MTR_4g024745 40.502 279 152 9 117 391 12 280 1.75e-42 151
MS.gene63221.t1 MTR_8g467790 32.240 366 189 12 1 355 174 491 2.64e-40 150
MS.gene63221.t1 MTR_8g077460 43.005 193 104 4 4 191 38 229 1.41e-38 143
MS.gene63221.t1 MTR_8g077460 33.829 269 148 9 155 415 129 375 1.15e-29 119
MS.gene63221.t1 MTR_5g021550 47.414 116 58 3 4 116 47 162 1.98e-25 101
MS.gene63221.t1 MTR_8g014290 41.463 123 63 4 4 117 38 160 2.50e-22 93.2
MS.gene63221.t1 MTR_5g080710 29.017 417 256 18 2 407 59 446 8.81e-22 97.4
MS.gene63221.t1 MTR_2g086020 40.667 150 68 5 1 142 1 137 2.47e-21 94.4
MS.gene63221.t1 MTR_2g086020 42.202 109 61 1 307 415 209 315 3.17e-18 85.5
MS.gene63221.t1 MTR_4g113810 32.000 175 102 6 57 221 1 168 9.70e-18 80.5
MS.gene63221.t1 MTR_5g084070 27.110 391 238 16 2 380 39 394 3.64e-17 83.6
MS.gene63221.t1 MTR_4g024720 36.296 135 61 6 4 116 45 176 1.58e-15 75.5
MS.gene63221.t1 MTR_8g014220 40.323 124 62 5 43 161 44 160 2.97e-15 73.6
MS.gene63221.t1 MTR_1g041245 35.465 172 88 6 2 166 37 192 3.42e-15 77.0
MS.gene63221.t1 MTR_7g033915 26.761 426 201 18 2 414 37 364 1.17e-14 75.5
MS.gene63221.t1 MTR_3g107100 25.926 432 232 18 2 415 37 398 1.40e-14 75.5
MS.gene63221.t1 MTR_4g056040 28.045 353 215 14 41 372 99 433 1.69e-14 75.5
MS.gene63221.t1 MTR_8g014230 50.685 73 32 2 333 405 27 95 1.55e-12 63.9
MS.gene63221.t1 MTR_7g098510 32.374 139 87 2 282 415 255 391 6.50e-12 67.0
MS.gene63221.t1 MTR_3g108560 25.359 418 232 15 2 414 46 388 8.08e-12 67.0
MS.gene63221.t1 MTR_7g098570 29.057 265 162 9 164 411 134 389 1.26e-11 66.6
MS.gene63221.t1 MTR_7g032380 32.394 142 86 3 281 415 218 356 2.29e-11 65.1
MS.gene63221.t1 MTR_4g056020 26.810 373 229 16 26 372 49 403 4.61e-11 64.7
MS.gene63221.t1 MTR_5g008950 26.582 237 142 6 200 415 140 365 4.72e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63221.t1 AT5G56420 25.522 431 264 15 2 415 32 422 2.19e-23 101
MS.gene63221.t1 AT5G56420 25.522 431 264 15 2 415 32 422 2.19e-23 101
MS.gene63221.t1 AT5G56420 25.522 431 264 15 2 415 32 422 2.19e-23 101
MS.gene63221.t1 AT4G26340 27.739 429 258 18 2 415 28 419 6.07e-23 100
MS.gene63221.t1 AT1G16930 26.503 449 255 19 2 415 41 449 7.88e-22 97.4
MS.gene63221.t1 AT1G16930 26.503 449 255 19 2 415 41 449 7.88e-22 97.4
MS.gene63221.t1 AT1G16930 26.503 449 255 19 2 415 41 449 7.88e-22 97.4
MS.gene63221.t1 AT5G56370 26.328 433 259 15 2 414 28 420 7.71e-21 94.4
MS.gene63221.t1 AT5G56370 26.328 433 259 15 2 414 28 420 7.71e-21 94.4
MS.gene63221.t1 AT5G62970 27.949 390 237 15 41 414 87 448 2.14e-20 93.2
MS.gene63221.t1 AT5G56700 28.493 365 221 15 41 392 22 359 3.33e-19 89.0
MS.gene63221.t1 AT1G32375 24.884 430 270 14 3 415 29 422 5.68e-18 85.9
MS.gene63221.t1 AT1G32375 24.884 430 270 14 3 415 29 422 5.68e-18 85.9
MS.gene63221.t1 AT5G60610 27.595 395 236 18 2 381 28 387 6.27e-17 82.4
MS.gene63221.t1 AT5G60610 27.595 395 236 18 2 381 28 387 6.27e-17 82.4
MS.gene63221.t1 AT2G26030 25.000 448 260 19 2 412 30 438 1.29e-16 82.0
MS.gene63221.t1 AT2G26030 25.000 448 260 19 2 412 30 438 1.29e-16 82.0
MS.gene63221.t1 AT5G53635 25.114 438 263 18 3 414 27 425 1.52e-16 81.6
MS.gene63221.t1 AT2G26030 24.283 453 262 20 2 412 30 443 2.95e-16 80.9
MS.gene63221.t1 AT4G00160 28.539 438 254 18 2 415 42 444 3.63e-16 80.5
MS.gene63221.t1 AT4G10420 25.434 346 224 14 77 414 2 321 3.81e-16 79.3
MS.gene63221.t1 AT4G10400 24.528 424 266 15 2 414 28 408 3.84e-16 80.1
MS.gene63221.t1 AT4G10400 24.528 424 266 15 2 414 28 408 3.84e-16 80.1
MS.gene63221.t1 AT4G10400 24.528 424 266 15 2 414 28 408 3.84e-16 80.1
MS.gene63221.t1 AT2G04230 27.681 401 239 15 2 381 39 409 6.72e-16 79.7
MS.gene63221.t1 AT5G53840 24.057 424 279 18 2 412 44 437 6.80e-16 79.7
MS.gene63221.t1 AT5G22720 25.446 448 267 21 2 414 49 464 7.08e-16 79.7
MS.gene63221.t1 AT5G38570 30.420 286 163 12 139 414 116 375 8.12e-16 79.0
MS.gene63221.t1 AT5G56400 25.635 433 266 19 2 415 60 455 2.28e-15 78.2
MS.gene63221.t1 AT5G38590 26.196 397 234 18 2 380 28 383 3.79e-15 77.0
MS.gene63221.t1 AT3G50710 24.828 435 269 16 3 415 29 427 4.52e-15 77.0
MS.gene63221.t1 AT3G54910 26.500 400 245 17 29 415 10 373 6.71e-15 76.3
MS.gene63221.t1 AT3G54910 26.500 400 245 17 29 415 10 373 6.71e-15 76.3
MS.gene63221.t1 AT3G54910 26.500 400 245 17 29 415 10 373 6.71e-15 76.3
MS.gene63221.t1 AT3G52680 27.955 440 252 20 2 415 47 447 1.60e-14 75.5
MS.gene63221.t1 AT3G49480 26.625 323 188 12 82 381 116 412 1.66e-14 75.5
MS.gene63221.t1 AT4G13985 23.502 434 289 15 1 412 44 456 4.79e-14 73.9
MS.gene63221.t1 AT5G56410 24.819 415 245 17 2 381 29 411 7.05e-14 73.6
MS.gene63221.t1 AT5G22670 27.005 374 223 17 27 376 59 406 8.33e-14 73.2
MS.gene63221.t1 AT5G56820 27.246 334 185 16 101 412 126 423 1.52e-13 72.4
MS.gene63221.t1 AT5G56390 25.227 440 256 18 3 414 30 424 2.67e-13 71.6
MS.gene63221.t1 AT1G66290 25.057 439 258 22 2 412 55 450 2.76e-13 71.6
MS.gene63221.t1 AT2G26860 26.173 405 239 18 2 381 28 397 4.91e-13 70.9
MS.gene63221.t1 AT2G26860 26.173 405 239 18 2 381 28 397 4.91e-13 70.9
MS.gene63221.t1 AT1G13780 24.379 443 277 19 2 412 36 452 5.13e-13 70.9
MS.gene63221.t1 AT4G14103 30.612 245 144 11 2 234 34 264 6.45e-13 70.1
MS.gene63221.t1 AT4G14103 30.612 245 144 11 2 234 34 264 6.45e-13 70.1
MS.gene63221.t1 AT4G14103 30.612 245 144 11 2 234 34 264 1.00e-12 70.1
MS.gene63221.t1 AT5G22610 23.438 448 280 18 2 413 44 464 1.26e-12 69.7
MS.gene63221.t1 AT3G26930 25.517 435 267 18 1 415 1 398 1.98e-12 68.9
MS.gene63221.t1 AT3G26930 25.517 435 267 18 1 415 46 443 2.34e-12 68.9
MS.gene63221.t1 AT2G26030 25.505 396 227 18 54 412 1 365 3.02e-12 68.2
MS.gene63221.t1 AT1G66310 23.799 437 265 18 2 412 45 439 3.35e-12 68.2
MS.gene63221.t1 AT1G22000 24.091 440 260 17 2 412 55 449 3.86e-12 68.2
MS.gene63221.t1 AT3G49480 26.804 291 168 11 110 381 8 272 3.94e-12 67.4
MS.gene63221.t1 AT5G50270 25.369 406 256 17 3 391 30 405 5.12e-12 67.8
MS.gene63221.t1 AT5G22610 22.581 434 271 17 2 413 44 434 5.26e-12 67.8
MS.gene63221.t1 AT5G44980 26.485 404 238 22 2 378 30 401 6.21e-12 67.4
MS.gene63221.t1 AT5G44980 26.485 404 238 22 2 378 30 401 6.21e-12 67.4
MS.gene63221.t1 AT3G26922 30.996 271 148 13 2 259 11 255 1.20e-11 65.5
MS.gene63221.t1 AT3G26922 30.996 271 148 13 2 259 11 255 1.20e-11 65.5
MS.gene63221.t1 AT3G26922 30.996 271 148 13 2 259 40 284 1.51e-11 65.5
MS.gene63221.t1 AT3G26922 30.996 271 148 13 2 259 40 284 1.51e-11 65.5
MS.gene63221.t1 AT5G56810 31.077 325 169 17 113 414 143 435 1.60e-11 66.2
MS.gene63221.t1 AT5G44950 24.700 417 261 21 2 393 30 418 1.66e-11 66.2
MS.gene63221.t1 AT4G13965 26.588 425 258 17 2 412 36 420 3.79e-11 65.1
MS.gene63221.t1 AT3G26922 34.135 208 109 10 65 259 67 259 3.97e-11 63.9
MS.gene63221.t1 AT5G27750 25.701 428 237 20 2 381 31 425 6.30e-11 64.3

Find 92 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACCTATATTCACTTTAA+AGG 0.147428 8.4:+41880392 MS.gene63221:CDS
TTGAAGACAATGCTTATTTC+TGG 0.178189 8.4:+41880476 MS.gene63221:CDS
GTTGTACTTTGCATGCTTCT+TGG 0.227885 8.4:-41880516 None:intergenic
TTTGGCAATGCCACTTCTAT+TGG 0.230747 8.4:+41879804 MS.gene63221:CDS
GAGGACGCCGTTGACGAATA+TGG 0.282809 8.4:-41879165 None:intergenic
CGTCTCTCTTGCGGCCAATA+TGG 0.307804 8.4:+41879221 MS.gene63221:CDS
AGTTCCAAAGTTTCTAAGAT+TGG 0.318329 8.4:-41879639 None:intergenic
TACCAAAAGAGGAGATTATT+AGG 0.323639 8.4:+41880060 MS.gene63221:intron
AAGACACTACAACTAGAAAT+TGG 0.343256 8.4:+41879573 MS.gene63221:CDS
ATTCACTTTAAAGGATATAT+AGG 0.344348 8.4:+41880401 MS.gene63221:CDS
TTCGGCCTCTCCATGGAAAT+CGG 0.345674 8.4:-41879126 None:intergenic
TGGTGAAATATTTGTGGAAT+TGG 0.346093 8.4:+41879518 MS.gene63221:CDS
TTGGCAATGCCACTTCTATT+GGG 0.365426 8.4:+41879805 MS.gene63221:CDS
AAGTGGGATACTAGACAATT+AGG 0.367479 8.4:-41880371 None:intergenic
GTTAACGTACACAGTCTCTA+TGG 0.377657 8.4:+41879498 MS.gene63221:intron
CCGAATCTTTGGCCAAGCTT+AGG 0.388638 8.4:+41879673 MS.gene63221:CDS
CGAATCTTTGGCCAAGCTTA+GGG 0.393635 8.4:+41879674 MS.gene63221:CDS
AGCCTCAACAATATCACATT+TGG 0.395052 8.4:+41879786 MS.gene63221:CDS
TGCGAAGTACCCGCGACATT+CGG 0.408630 8.4:+41879192 MS.gene63221:CDS
TCGGCCTCTCCATGGAAATC+GGG 0.416365 8.4:-41879125 None:intergenic
CGTGGATCCGCACCGCCGTT+GGG 0.424276 8.4:+41879276 MS.gene63221:CDS
CTCATAGGTATCTTCATCTC+TGG 0.437215 8.4:+41879063 MS.gene63221:CDS
GTTTAATGCCAGAGAGAGCT+TGG 0.438724 8.4:-41879911 None:intergenic
CCTAAGCTTGGCCAAAGATT+CGG 0.439078 8.4:-41879673 None:intergenic
TGAAGAAATCAAGTCCATAT+TGG 0.439919 8.4:-41879235 None:intergenic
GGCCAAAGATTCGGTTGAAA+AGG 0.442677 8.4:-41879664 None:intergenic
AGAGGAGGTTAGTGCATGAT+AGG 0.446173 8.4:-41879370 None:intergenic
TTGTCCAATCTTAGAAACTT+TGG 0.454352 8.4:+41879635 MS.gene63221:CDS
TGAAGCACGTCGTACAGAAA+AGG 0.458324 8.4:-41880171 None:intergenic
GCTCTCCTCCAAGCTCTCTC+TGG 0.463060 8.4:+41879903 MS.gene63221:CDS
AGTTGTAGTGTCTTCAATGA+TGG 0.466751 8.4:-41879564 None:intergenic
GCACTAACCTCCTCTCCATC+AGG 0.467643 8.4:+41879378 MS.gene63221:CDS
CAATGAGTCTTGTCTCTCAT+AGG 0.474674 8.4:+41879048 None:intergenic
ATTATGAGAGCTTGAAGTAT+AGG 0.479156 8.4:-41880201 None:intergenic
ACGTGGATCCGCACCGCCGT+TGG 0.482549 8.4:+41879275 MS.gene63221:CDS
TCTCTATGGTGAAATATTTG+TGG 0.484639 8.4:+41879512 MS.gene63221:CDS
CTCGCTCTCTTCACGTCTGA+GGG 0.487168 8.4:+41879320 MS.gene63221:CDS
ATATCCTTTAAAGTGAATAT+AGG 0.491831 8.4:-41880396 None:intergenic
CATCGTAGAAATCAAAAGCT+TGG 0.492563 8.4:-41879094 None:intergenic
TCTCGCTCTCTTCACGTCTG+AGG 0.498144 8.4:+41879319 MS.gene63221:CDS
CTTGGAGGAGAGCGAGGAAA+GGG 0.499254 8.4:-41879893 None:intergenic
ATGTGATATTGTTGAGGCTA+AGG 0.505883 8.4:-41879782 None:intergenic
GTGTATTACCCCGATTTCCA+TGG 0.507047 8.4:+41879116 MS.gene63221:CDS
TAACTTAGTCAAATTTAACT+TGG 0.510908 8.4:-41880583 None:intergenic
ATCCTCAAGGTGGGGCCCAA+CGG 0.512786 8.4:-41879291 None:intergenic
GAAGCACGTCGTACAGAAAA+GGG 0.514480 8.4:-41880170 None:intergenic
AGGTTAGTGCATGATAGGAC+CGG 0.527359 8.4:-41879365 None:intergenic
CTCAAGGTGGGGCCCAACGG+CGG 0.527894 8.4:-41879288 None:intergenic
CGGCTTCAAACTCCCTCTCC+CGG 0.528625 8.4:+41879346 MS.gene63221:CDS
CGCCGTTGGGCCCCACCTTG+AGG 0.530412 8.4:+41879289 MS.gene63221:CDS
GCTTGGAGGAGAGCGAGGAA+AGG 0.544578 8.4:-41879894 None:intergenic
TAAAGTGAATATAGGTCAAG+TGG 0.551062 8.4:-41880388 None:intergenic
AACAGATTCACTTTCAGAAG+AGG 0.560957 8.4:-41879868 None:intergenic
GGTGGGGCCCAACGGCGGTG+CGG 0.567700 8.4:-41879283 None:intergenic
ACGCATCTTCCGAATGTCGC+GGG 0.569466 8.4:-41879201 None:intergenic
TTAAAGGATATATAGGACAT+TGG 0.572576 8.4:+41880408 MS.gene63221:CDS
ACAACTAGAAATTGGTAATG+TGG 0.572971 8.4:+41879581 MS.gene63221:CDS
GGAAGATGGAACCCTAAGCT+TGG 0.579963 8.4:-41879685 None:intergenic
CTCTTCACGTCTGAGGGTGA+CGG 0.581747 8.4:+41879326 MS.gene63221:CDS
AGATTGGACAAGCAGAGAGA+AGG 0.584782 8.4:-41879623 None:intergenic
GTGCATGATAGGACCGGGAG+AGG 0.587136 8.4:-41879359 None:intergenic
TAATGCCAGAGAGAGCTTGG+AGG 0.588199 8.4:-41879908 None:intergenic
AGAGAGCTTGGAGGAGAGCG+AGG 0.588788 8.4:-41879899 None:intergenic
GTGCAAGTTCCCAATAGAAG+TGG 0.589578 8.4:-41879814 None:intergenic
GACTTTGCCATATTCGTCAA+CGG 0.590231 8.4:+41879158 MS.gene63221:CDS
TTTGTTACCTGATGGAGAGG+AGG 0.590784 8.4:-41879385 None:intergenic
TGTCTTGAGATTGACACACC+TGG 0.595030 8.4:+41879750 MS.gene63221:CDS
GTTTCGTTGTTATGCAGCAA+TGG 0.597532 8.4:+41879941 MS.gene63221:CDS
CGCGGGTACTTCGCATAGAG+AGG 0.601043 8.4:-41879184 None:intergenic
AGATTTGTTACCTGATGGAG+AGG 0.603999 8.4:-41879388 None:intergenic
GAGATGAAGATCCTCAAGGT+GGG 0.607880 8.4:-41879300 None:intergenic
TGCATGATAGGACCGGGAGA+GGG 0.607937 8.4:-41879358 None:intergenic
CGGAAGATGCGTCTCTCTTG+CGG 0.610368 8.4:+41879212 MS.gene63221:CDS
TGGCAGACAGTAGATCCCCT+TGG 0.612624 8.4:-41880563 None:intergenic
GGTTAGTGCATGATAGGACC+GGG 0.613486 8.4:-41879364 None:intergenic
CTGAACGCTCTGTTGACACG+TGG 0.616550 8.4:+41879258 MS.gene63221:CDS
AGGCTAAGGTACTTGAGACC+AGG 0.622331 8.4:-41879768 None:intergenic
CGGCCTCTCCATGGAAATCG+GGG 0.624754 8.4:-41879124 None:intergenic
TACAGAAAAGGGTCGACAGT+GGG 0.624804 8.4:-41880159 None:intergenic
TTACCCCGATTTCCATGGAG+AGG 0.627717 8.4:+41879121 MS.gene63221:CDS
ACGAGTTAAATATAGTCTCA+CGG 0.631410 8.4:+41880331 MS.gene63221:CDS
AGATGAAGATCCTCAAGGTG+GGG 0.631714 8.4:-41879299 None:intergenic
AAAGTGAATATAGGTCAAGT+GGG 0.636598 8.4:-41880387 None:intergenic
TGCCAAATGTGATATTGTTG+AGG 0.639120 8.4:-41879788 None:intergenic
AGCAGAGAGAAGGATATCAA+CGG 0.645343 8.4:-41879613 None:intergenic
GACGCATCTTCCGAATGTCG+CGG 0.645697 8.4:-41879202 None:intergenic
CGAGATGAAGATCCTCAAGG+TGG 0.647298 8.4:-41879301 None:intergenic
GAGCGAGATGAAGATCCTCA+AGG 0.648810 8.4:-41879304 None:intergenic
GTACAGAAAAGGGTCGACAG+TGG 0.663564 8.4:-41880160 None:intergenic
TATTGGGAACTTGCACAACG+TGG 0.670170 8.4:+41879821 MS.gene63221:CDS
TGGGAACTTGCACAACGTGG+AGG 0.687506 8.4:+41879824 MS.gene63221:CDS
TCTCATAATTCATAATGACA+AGG 0.702264 8.4:+41880215 MS.gene63221:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTAAATTTAAAACTTTTCA+AGG - chr8.4:41879709-41879728 None:intergenic 15.0%
!!! ATTTTCGAAAAATCTATAAT+AGG - chr8.4:41880093-41880112 None:intergenic 15.0%
!! AATGGAATATATAATACACA+AGG - chr8.4:41879986-41880005 None:intergenic 20.0%
!!! ATATCCTTTAAAGTGAATAT+AGG - chr8.4:41880399-41880418 None:intergenic 20.0%
!!! ATTCACTTTAAAGGATATAT+AGG + chr8.4:41880401-41880420 MS.gene63221:CDS 20.0%
!!! TGTGTTTTTGTTTGATTATT+TGG + chr8.4:41880276-41880295 MS.gene63221:intron 20.0%
! TCTCATAATTCATAATGACA+AGG + chr8.4:41880215-41880234 MS.gene63221:CDS 25.0%
! TTAAAGGATATATAGGACAT+TGG + chr8.4:41880408-41880427 MS.gene63221:CDS 25.0%
! TTGACCTATATTCACTTTAA+AGG + chr8.4:41880392-41880411 MS.gene63221:CDS 25.0%
!! AAAATTTTCGAATATGCCAA+GGG + chr8.4:41880547-41880566 MS.gene63221:CDS 25.0%
!! TTTAACTTAAGCTTAAGTGT+GGG + chr8.4:41880251-41880270 MS.gene63221:intron 25.0%
!!! TTAAAACTTTTCAAGGAAGA+TGG - chr8.4:41879702-41879721 None:intergenic 25.0%
!!! TTTTTCGAAAATGCACTTTT+CGG + chr8.4:41880101-41880120 MS.gene63221:CDS 25.0%
AAAGTGAATATAGGTCAAGT+GGG - chr8.4:41880390-41880409 None:intergenic 30.0%
AAGACACTACAACTAGAAAT+TGG + chr8.4:41879573-41879592 MS.gene63221:CDS 30.0%
ACGAGTTAAATATAGTCTCA+CGG + chr8.4:41880331-41880350 MS.gene63221:CDS 30.0%
ATTATGAGAGCTTGAAGTAT+AGG - chr8.4:41880204-41880223 None:intergenic 30.0%
TAAAGTGAATATAGGTCAAG+TGG - chr8.4:41880391-41880410 None:intergenic 30.0%
TACCAAAAGAGGAGATTATT+AGG + chr8.4:41880060-41880079 MS.gene63221:intron 30.0%
TCTCTATGGTGAAATATTTG+TGG + chr8.4:41879512-41879531 MS.gene63221:CDS 30.0%
TGAAGAAATCAAGTCCATAT+TGG - chr8.4:41879238-41879257 None:intergenic 30.0%
TGGTGAAATATTTGTGGAAT+TGG + chr8.4:41879518-41879537 MS.gene63221:CDS 30.0%
TTGAAGACAATGCTTATTTC+TGG + chr8.4:41880476-41880495 MS.gene63221:CDS 30.0%
TTGTCCAATCTTAGAAACTT+TGG + chr8.4:41879635-41879654 MS.gene63221:CDS 30.0%
! AAATTTTCGAATATGCCAAG+GGG + chr8.4:41880548-41880567 MS.gene63221:CDS 30.0%
! AGACAATTAGGAACACTTTT+CGG - chr8.4:41880362-41880381 None:intergenic 30.0%
! GAAAATTTTCGAATATGCCA+AGG + chr8.4:41880546-41880565 MS.gene63221:CDS 30.0%
! GTTTAACTTAAGCTTAAGTG+TGG + chr8.4:41880250-41880269 MS.gene63221:intron 30.0%
! TACCTAATAATCTCCTCTTT+TGG - chr8.4:41880065-41880084 None:intergenic 30.0%
!! ACAAAAGATTTGTTACCTGA+TGG - chr8.4:41879396-41879415 None:intergenic 30.0%
!! ACAACTAGAAATTGGTAATG+TGG + chr8.4:41879581-41879600 MS.gene63221:CDS 30.0%
!! AGTTCCAAAGTTTCTAAGAT+TGG - chr8.4:41879642-41879661 None:intergenic 30.0%
!!! GGCAAAGTCTTTGAAATTTT+CGG - chr8.4:41879147-41879166 None:intergenic 30.0%
AACAGATTCACTTTCAGAAG+AGG - chr8.4:41879871-41879890 None:intergenic 35.0%
AAGTGGGATACTAGACAATT+AGG - chr8.4:41880374-41880393 None:intergenic 35.0%
AGCCTCAACAATATCACATT+TGG + chr8.4:41879786-41879805 MS.gene63221:CDS 35.0%
AGTTGTAGTGTCTTCAATGA+TGG - chr8.4:41879567-41879586 None:intergenic 35.0%
ATGTGATATTGTTGAGGCTA+AGG - chr8.4:41879785-41879804 None:intergenic 35.0%
CATCGTAGAAATCAAAAGCT+TGG - chr8.4:41879097-41879116 None:intergenic 35.0%
TGCCAAATGTGATATTGTTG+AGG - chr8.4:41879791-41879810 None:intergenic 35.0%
! GCTAGTTATGTTTTGCAGTA+TGG + chr8.4:41880446-41880465 MS.gene63221:CDS 35.0%
! TTCCTTTTCAACCGAATCTT+TGG + chr8.4:41879662-41879681 MS.gene63221:CDS 35.0%
!! AGTTGTAATTTGTTTCCTGC+AGG + chr8.4:41880301-41880320 MS.gene63221:intron 35.0%
AGCAGAGAGAAGGATATCAA+CGG - chr8.4:41879616-41879635 None:intergenic 40.0%
CTCATAGGTATCTTCATCTC+TGG + chr8.4:41879063-41879082 MS.gene63221:CDS 40.0%
GACTTTGCCATATTCGTCAA+CGG + chr8.4:41879158-41879177 MS.gene63221:CDS 40.0%
GTTAACGTACACAGTCTCTA+TGG + chr8.4:41879498-41879517 MS.gene63221:intron 40.0%
GTTGTACTTTGCATGCTTCT+TGG - chr8.4:41880519-41880538 None:intergenic 40.0%
GTTTCGTTGTTATGCAGCAA+TGG + chr8.4:41879941-41879960 MS.gene63221:CDS 40.0%
TGTTGTGTACGTACCAAAAG+AGG + chr8.4:41880049-41880068 MS.gene63221:intron 40.0%
! TTGGCAATGCCACTTCTATT+GGG + chr8.4:41879805-41879824 MS.gene63221:CDS 40.0%
! TTTGGCAATGCCACTTCTAT+TGG + chr8.4:41879804-41879823 MS.gene63221:CDS 40.0%
!! AGATTTGTTACCTGATGGAG+AGG - chr8.4:41879391-41879410 None:intergenic 40.0%
AGAGGAGGTTAGTGCATGAT+AGG - chr8.4:41879373-41879392 None:intergenic 45.0%
AGATGAAGATCCTCAAGGTG+GGG - chr8.4:41879302-41879321 None:intergenic 45.0%
AGATTGGACAAGCAGAGAGA+AGG - chr8.4:41879626-41879645 None:intergenic 45.0%
AGGTTAGTGCATGATAGGAC+CGG - chr8.4:41879368-41879387 None:intergenic 45.0%
CCTAAGCTTGGCCAAAGATT+CGG - chr8.4:41879676-41879695 None:intergenic 45.0%
CGAATCTTTGGCCAAGCTTA+GGG + chr8.4:41879674-41879693 MS.gene63221:CDS 45.0%
GAAGCACGTCGTACAGAAAA+GGG - chr8.4:41880173-41880192 None:intergenic 45.0%
GAGATGAAGATCCTCAAGGT+GGG - chr8.4:41879303-41879322 None:intergenic 45.0%
GGTACGTACACAACAACAAG+TGG - chr8.4:41880044-41880063 None:intergenic 45.0%
GTGCAAGTTCCCAATAGAAG+TGG - chr8.4:41879817-41879836 None:intergenic 45.0%
GTGTATTACCCCGATTTCCA+TGG + chr8.4:41879116-41879135 MS.gene63221:CDS 45.0%
GTTTAATGCCAGAGAGAGCT+TGG - chr8.4:41879914-41879933 None:intergenic 45.0%
TAACTCGTGATGTATCCTGC+AGG - chr8.4:41880319-41880338 None:intergenic 45.0%
TACAGAAAAGGGTCGACAGT+GGG - chr8.4:41880162-41880181 None:intergenic 45.0%
TATTGGGAACTTGCACAACG+TGG + chr8.4:41879821-41879840 MS.gene63221:CDS 45.0%
TGAAGCACGTCGTACAGAAA+AGG - chr8.4:41880174-41880193 None:intergenic 45.0%
TGTCTTGAGATTGACACACC+TGG + chr8.4:41879750-41879769 MS.gene63221:CDS 45.0%
TTTGTTACCTGATGGAGAGG+AGG - chr8.4:41879388-41879407 None:intergenic 45.0%
! GGCCAAAGATTCGGTTGAAA+AGG - chr8.4:41879667-41879686 None:intergenic 45.0%
! TGAAATTTTCGGCCTCTCCA+TGG - chr8.4:41879136-41879155 None:intergenic 45.0%
!!! CAAATTTTTTAAATATATAA+TGG - chr8.4:41880004-41880023 None:intergenic 5.0%
CCGAATCTTTGGCCAAGCTT+AGG + chr8.4:41879673-41879692 MS.gene63221:CDS 50.0%
CGAGATGAAGATCCTCAAGG+TGG - chr8.4:41879304-41879323 None:intergenic 50.0%
GAGCGAGATGAAGATCCTCA+AGG - chr8.4:41879307-41879326 None:intergenic 50.0%
GGAAGATGGAACCCTAAGCT+TGG - chr8.4:41879688-41879707 None:intergenic 50.0%
GGTTAGTGCATGATAGGACC+GGG - chr8.4:41879367-41879386 None:intergenic 50.0%
GTACAGAAAAGGGTCGACAG+TGG - chr8.4:41880163-41880182 None:intergenic 50.0%
TAATGCCAGAGAGAGCTTGG+AGG - chr8.4:41879911-41879930 None:intergenic 50.0%
TTACCCCGATTTCCATGGAG+AGG + chr8.4:41879121-41879140 MS.gene63221:CDS 50.0%
TTCGGCCTCTCCATGGAAAT+CGG - chr8.4:41879129-41879148 None:intergenic 50.0%
! AGGCTAAGGTACTTGAGACC+AGG - chr8.4:41879771-41879790 None:intergenic 50.0%
ACGCATCTTCCGAATGTCGC+GGG - chr8.4:41879204-41879223 None:intergenic 55.0%
CGGAAGATGCGTCTCTCTTG+CGG + chr8.4:41879212-41879231 MS.gene63221:CDS 55.0%
CGTCTCTCTTGCGGCCAATA+TGG + chr8.4:41879221-41879240 MS.gene63221:CDS 55.0%
CTCGCTCTCTTCACGTCTGA+GGG + chr8.4:41879320-41879339 MS.gene63221:CDS 55.0%
CTCTTCACGTCTGAGGGTGA+CGG + chr8.4:41879326-41879345 MS.gene63221:CDS 55.0%
CTGAACGCTCTGTTGACACG+TGG + chr8.4:41879258-41879277 MS.gene63221:CDS 55.0%
CTTGGAGGAGAGCGAGGAAA+GGG - chr8.4:41879896-41879915 None:intergenic 55.0%
GACGCATCTTCCGAATGTCG+CGG - chr8.4:41879205-41879224 None:intergenic 55.0%
GAGGACGCCGTTGACGAATA+TGG - chr8.4:41879168-41879187 None:intergenic 55.0%
GCACTAACCTCCTCTCCATC+AGG + chr8.4:41879378-41879397 MS.gene63221:CDS 55.0%
TCGGCCTCTCCATGGAAATC+GGG - chr8.4:41879128-41879147 None:intergenic 55.0%
TCTCGCTCTCTTCACGTCTG+AGG + chr8.4:41879319-41879338 MS.gene63221:CDS 55.0%
TGCATGATAGGACCGGGAGA+GGG - chr8.4:41879361-41879380 None:intergenic 55.0%
TGGCAGACAGTAGATCCCCT+TGG - chr8.4:41880566-41880585 None:intergenic 55.0%
TGGGAACTTGCACAACGTGG+AGG + chr8.4:41879824-41879843 MS.gene63221:CDS 55.0%
! TGCGAAGTACCCGCGACATT+CGG + chr8.4:41879192-41879211 MS.gene63221:CDS 55.0%
AGAGAGCTTGGAGGAGAGCG+AGG - chr8.4:41879902-41879921 None:intergenic 60.0%
ATCCTCAAGGTGGGGCCCAA+CGG - chr8.4:41879294-41879313 None:intergenic 60.0%
CGCGGGTACTTCGCATAGAG+AGG - chr8.4:41879187-41879206 None:intergenic 60.0%
CGGCCTCTCCATGGAAATCG+GGG - chr8.4:41879127-41879146 None:intergenic 60.0%
CGGCTTCAAACTCCCTCTCC+CGG + chr8.4:41879346-41879365 MS.gene63221:CDS 60.0%
GCTCTCCTCCAAGCTCTCTC+TGG + chr8.4:41879903-41879922 MS.gene63221:CDS 60.0%
GCTTGGAGGAGAGCGAGGAA+AGG - chr8.4:41879897-41879916 None:intergenic 60.0%
GTGCATGATAGGACCGGGAG+AGG - chr8.4:41879362-41879381 None:intergenic 60.0%
ACGTGGATCCGCACCGCCGT+TGG + chr8.4:41879275-41879294 MS.gene63221:CDS 70.0%
CGTGGATCCGCACCGCCGTT+GGG + chr8.4:41879276-41879295 MS.gene63221:CDS 70.0%
CTCAAGGTGGGGCCCAACGG+CGG - chr8.4:41879291-41879310 None:intergenic 70.0%
CGCCGTTGGGCCCCACCTTG+AGG + chr8.4:41879289-41879308 MS.gene63221:CDS 75.0%
! GGTGGGGCCCAACGGCGGTG+CGG - chr8.4:41879286-41879305 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 41879050 41880601 41879050 ID=MS.gene63221
chr8.4 mRNA 41879050 41880601 41879050 ID=MS.gene63221.t1;Parent=MS.gene63221
chr8.4 exon 41879050 41879399 41879050 ID=MS.gene63221.t1.exon1;Parent=MS.gene63221.t1
chr8.4 CDS 41879050 41879399 41879050 ID=cds.MS.gene63221.t1;Parent=MS.gene63221.t1
chr8.4 exon 41879512 41879962 41879512 ID=MS.gene63221.t1.exon2;Parent=MS.gene63221.t1
chr8.4 CDS 41879512 41879962 41879512 ID=cds.MS.gene63221.t1;Parent=MS.gene63221.t1
chr8.4 exon 41880069 41880236 41880069 ID=MS.gene63221.t1.exon3;Parent=MS.gene63221.t1
chr8.4 CDS 41880069 41880236 41880069 ID=cds.MS.gene63221.t1;Parent=MS.gene63221.t1
chr8.4 exon 41880323 41880601 41880323 ID=MS.gene63221.t1.exon4;Parent=MS.gene63221.t1
chr8.4 CDS 41880323 41880601 41880323 ID=cds.MS.gene63221.t1;Parent=MS.gene63221.t1
Gene Sequence

>MS.gene63221

ATGAGTCTTGTCTCTCATAGGTATCTTCATCTCTGGAAACATCTCCAAGCTTTTGATTTCTACGATGTGTATTACCCCGATTTCCATGGAGAGGCCGAAAATTTCAAAGACTTTGCCATATTCGTCAACGGCGTCCTCTCTATGCGAAGTACCCGCGACATTCGGAAGATGCGTCTCTCTTGCGGCCAATATGGACTTGATTTCTTCACTGAACGCTCTGTTGACACGTGGATCCGCACCGCCGTTGGGCCCCACCTTGAGGATCTTCATCTCGCTCTCTTCACGTCTGAGGGTGACGGCTTCAAACTCCCTCTCCCGGTCCTATCATGCACTAACCTCCTCTCCATCAGGTAACAAATCTTTTGTCAAACAATCTTCTTTTTGTACAGTTATTATTTGCACAGTTATTCTTGTTTAAATTAAAATGTGACATAATAAATGAAGATTTGTTAACGTACACAGTCTCTATGGTGAAATATTTGTGGAATTGGAACAATCTTCGCGTGTTTGTTTACCATCATTGAAGACACTACAACTAGAAATTGGTAATGTGGATGTGAATTCCGTTGATATCCTTCTCTCTGCTTGTCCAATCTTAGAAACTTTGGAACTTTCCTTTTCAACCGAATCTTTGGCCAAGCTTAGGGTTCCATCTTCCTTGAAAAGTTTTAAATTTACTATAGAGAATGACACTGAAGTTTGTCTTGAGATTGACACACCTGGTCTCAAGTACCTTAGCCTCAACAATATCACATTTGGCAATGCCACTTCTATTGGGAACTTGCACAACGTGGAGGAAGCATATCTTGATGTACTCTCCTCTTCTGAAAGTGAATCTGTTGACCCTTTCCTCGCTCTCCTCCAAGCTCTCTCTGGCATTAAACGTCTAGTGTTTCGTTGTTATGCAGCAATGGTGAGTGAAATTTTCATTATCCTTGTGTATTATATATTCCATTATATATTTAAAAAATTTGTGCTAATTCTCGTATCTCCACTTGTTGTTGTGTACGTACCAAAAGAGGAGATTATTAGGTACTGAACCTATTATAGATTTTTCGAAAATGCACTTTTCGGAATTTCGCTGTTTACTACATCTAGAGCTCATTTTTCCCACTGTCGACCCTTTTCTGTACGACGTGCTTCAGAAATGTCCTATACTTCAAGCTCTCATAATTCATAATGACAAGGTTCGTAAATAGAGTTTAACTTAAGCTTAAGTGTGGGTGTGTGTTTTTGTTTGATTATTTGGTGAGTTGTAATTTGTTTCCTGCAGGATACATCACGAGTTAAATATAGTCTCACGGAAAAGCCGAAAAGTGTTCCTAATTGTCTAGTATCCCACTTGACCTATATTCACTTTAAAGGATATATAGGACATTGGCATGAGAAAGAATTTGCTAGTTATGTTTTGCAGTATGGACTTGTTTTGAAGACAATGCTTATTTCTGGTTTTTTGTCGAATGAAACCAAGAAGCATGCAAAGTACAACTATCGCAGAAAATTTTCGAATATGCCAAGGGGATCTACTGTCTGCCAAGTTAAATTTGACTAA
Protein sequence

>MS.gene63221.t1

MSLVSHRYLHLWKHLQAFDFYDVYYPDFHGEAENFKDFAIFVNGVLSMRSTRDIRKMRLSCGQYGLDFFTERSVDTWIRTAVGPHLEDLHLALFTSEGDGFKLPLPVLSCTNLLSISLYGEIFVELEQSSRVCLPSLKTLQLEIGNVDVNSVDILLSACPILETLELSFSTESLAKLRVPSSLKSFKFTIENDTEVCLEIDTPGLKYLSLNNITFGNATSIGNLHNVEEAYLDVLSSSESESVDPFLALLQALSGIKRLVFRCYAAMRRLLGTEPIIDFSKMHFSEFRCLLHLELIFPTVDPFLYDVLQKCPILQALIIHNDKDTSRVKYSLTEKPKSVPNCLVSHLTYIHFKGYIGHWHEKEFASYVLQYGLVLKTMLISGFLSNETKKHAKYNYRRKFSNMPRGSTVCQVKFD