AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63113

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63113.t1 MTR_8g062300 60.808 495 137 10 1 456 1 477 0.0 531
MS.gene63113.t1 MTR_8g070950 57.451 463 158 11 3 456 2 434 5.44e-159 457
MS.gene63113.t1 MTR_8g062010 58.850 452 157 12 12 456 1 430 1.80e-155 448
MS.gene63113.t1 MTR_8g062310 54.977 442 164 8 20 460 25 432 1.19e-151 438
MS.gene63113.t1 MTR_8g062080 54.348 460 174 11 8 456 11 445 2.62e-150 436
MS.gene63113.t1 MTR_8g469020 48.305 472 186 12 18 456 5 451 9.84e-135 396
MS.gene63113.t1 MTR_8g058965 49.269 479 187 14 11 456 1 456 2.17e-132 390
MS.gene63113.t1 MTR_4g050250 47.392 441 172 15 1 402 1 420 7.80e-111 334
MS.gene63113.t1 MTR_8g467790 45.340 397 152 11 20 401 148 494 4.04e-94 294
MS.gene63113.t1 MTR_8g468810 38.302 483 246 16 4 456 3 463 5.88e-74 240
MS.gene63113.t1 MTR_2g083840 37.474 475 248 14 6 456 8 457 7.03e-73 237
MS.gene63113.t1 MTR_8g062270 36.989 465 237 15 20 455 16 453 1.02e-72 236
MS.gene63113.t1 MTR_8g058970 37.271 491 254 17 4 456 3 477 1.53e-70 231
MS.gene63113.t1 MTR_2g086020 79.433 141 29 0 316 456 175 315 3.38e-68 221
MS.gene63113.t1 MTR_2g086020 51.923 156 70 3 45 198 1 153 1.08e-38 143
MS.gene63113.t1 MTR_8g014260 35.232 474 266 17 1 456 1 451 3.14e-66 219
MS.gene63113.t1 MTR_8g467800 35.565 478 259 15 8 460 4 457 1.40e-63 213
MS.gene63113.t1 MTR_8g468840 36.784 454 249 13 20 454 14 448 2.79e-63 212
MS.gene63113.t1 MTR_4g032730 33.402 488 260 13 8 455 4 466 7.90e-63 211
MS.gene63113.t1 MTR_5g092960 33.192 473 259 11 15 455 14 461 3.45e-59 201
MS.gene63113.t1 MTR_8g468760 34.461 473 226 15 12 455 1 418 1.33e-55 191
MS.gene63113.t1 MTR_8g468810 38.363 391 191 15 4 366 3 371 1.40e-52 181
MS.gene63113.t1 MTR_8g445780 36.207 406 215 15 20 409 13 390 2.71e-52 181
MS.gene63113.t1 MTR_8g468810 39.935 308 151 11 4 288 3 299 3.77e-44 157
MS.gene63113.t1 MTR_8g468810 39.935 308 151 11 4 288 3 299 4.68e-44 157
MS.gene63113.t1 MTR_8g468810 39.935 308 151 11 4 288 3 299 1.08e-43 157
MS.gene63113.t1 MTR_8g014160 30.989 455 226 13 20 456 41 425 1.97e-42 155
MS.gene63113.t1 MTR_5g092960 32.632 380 206 9 15 366 14 371 1.22e-38 144
MS.gene63113.t1 MTR_3g099800 70.115 87 25 1 368 453 19 105 1.13e-32 120
MS.gene63113.t1 MTR_8g467800 37.874 301 156 13 8 288 4 293 2.38e-32 126
MS.gene63113.t1 MTR_8g077460 39.171 217 115 8 20 225 10 220 6.93e-31 122
MS.gene63113.t1 MTR_8g077460 41.722 151 80 5 309 456 230 375 3.91e-26 109
MS.gene63113.t1 MTR_4g024710 39.500 200 104 6 258 456 164 347 8.20e-31 122
MS.gene63113.t1 MTR_5g021550 44.848 165 73 6 5 157 4 162 7.96e-30 114
MS.gene63113.t1 MTR_8g014210 38.500 200 102 7 260 456 50 231 2.47e-27 109
MS.gene63113.t1 MTR_8g014290 39.306 173 77 7 1 158 1 160 2.59e-25 102
MS.gene63113.t1 MTR_1g041245 27.333 450 240 15 20 456 11 386 2.68e-25 107
MS.gene63113.t1 MTR_7g026500 25.902 471 258 14 7 459 1 398 2.52e-24 104
MS.gene63113.t1 MTR_7g098510 25.751 466 261 17 1 456 1 391 4.37e-21 95.1
MS.gene63113.t1 MTR_2g007450 26.205 477 256 18 9 453 5 417 2.76e-20 93.2
MS.gene63113.t1 MTR_4g024745 30.249 281 148 11 170 431 25 276 3.42e-20 90.9
MS.gene63113.t1 MTR_5g084070 26.490 453 285 18 8 448 3 419 7.19e-20 92.0
MS.gene63113.t1 MTR_4g024720 40.000 130 66 5 4 126 3 127 2.71e-19 86.7
MS.gene63113.t1 MTR_5g080710 26.786 448 260 17 3 428 24 425 1.22e-18 88.6
MS.gene63113.t1 MTR_3g107100 24.406 463 268 19 10 456 2 398 1.31e-17 85.1
MS.gene63113.t1 MTR_6g045333 24.550 444 268 14 20 455 61 445 1.78e-16 81.6
MS.gene63113.t1 MTR_5g073420 31.937 191 119 6 20 205 11 195 2.20e-16 80.9
MS.gene63113.t1 MTR_7g056447 33.333 195 119 7 12 204 1 186 2.35e-16 79.7
MS.gene63113.t1 MTR_7g098480 26.782 463 256 16 1 455 1 388 3.09e-16 81.3
MS.gene63113.t1 MTR_2g007400 24.839 467 252 18 17 456 9 403 8.67e-16 79.3
MS.gene63113.t1 MTR_7g032380 34.359 195 109 6 20 207 11 193 9.25e-16 79.0
MS.gene63113.t1 MTR_5g007410 25.740 439 258 14 22 453 28 405 1.50e-15 79.0
MS.gene63113.t1 MTR_4g098590 24.706 425 234 13 22 427 1 358 2.63e-15 77.8
MS.gene63113.t1 MTR_1g108820 27.964 447 250 22 14 453 2 383 3.61e-15 77.4
MS.gene63113.t1 MTR_7g115270 31.401 207 119 7 20 213 12 208 6.47e-15 76.6
MS.gene63113.t1 MTR_1g053025 25.893 448 255 18 20 453 2 386 8.55e-15 76.3
MS.gene63113.t1 MTR_4g024770 50.000 64 29 1 1 64 8 68 2.30e-14 68.6
MS.gene63113.t1 MTR_1g492880 40.860 93 51 2 12 103 1 90 2.79e-14 68.9
MS.gene63113.t1 MTR_3g107120 32.338 201 114 10 20 205 9 202 2.88e-14 74.7
MS.gene63113.t1 MTR_1g492880 40.860 93 51 2 12 103 1 90 3.71e-14 68.9
MS.gene63113.t1 MTR_5g008950 30.000 210 132 7 22 230 1 196 6.80e-14 73.6
MS.gene63113.t1 MTR_2g008310 25.942 451 239 17 20 456 2 371 8.18e-14 73.2
MS.gene63113.t1 MTR_6g022500 23.908 435 228 16 10 423 91 443 1.46e-13 72.8
MS.gene63113.t1 MTR_2g007500 31.132 212 110 8 7 207 1 187 1.49e-13 72.0
MS.gene63113.t1 MTR_3g105060 30.303 198 124 8 20 213 14 201 3.19e-13 71.2
MS.gene63113.t1 MTR_8g014180 55.172 58 24 1 4 61 3 58 4.52e-13 64.3
MS.gene63113.t1 MTR_3g040700 28.934 197 121 7 19 207 42 227 1.12e-12 70.1
MS.gene63113.t1 MTR_2g008320 34.184 196 111 7 20 207 52 237 2.03e-12 69.3
MS.gene63113.t1 MTR_3g036250 29.534 193 125 6 19 207 4 189 2.09e-12 68.9
MS.gene63113.t1 MTR_7g034110 46.835 79 38 2 20 98 11 85 2.35e-12 68.6
MS.gene63113.t1 MTR_7g056433 33.010 206 123 7 7 207 1 196 2.76e-12 68.6
MS.gene63113.t1 MTR_7g033990 30.000 210 117 7 17 212 8 201 3.47e-12 68.6
MS.gene63113.t1 MTR_8g014230 40.000 85 47 2 365 449 18 98 5.96e-12 62.4
MS.gene63113.t1 MTR_3g023630 31.472 197 110 9 19 205 7 188 6.27e-12 67.4
MS.gene63113.t1 MTR_7g034115 32.990 194 117 6 18 207 9 193 2.02e-11 65.9
MS.gene63113.t1 MTR_1g107385 31.441 229 135 10 3 216 20 241 2.73e-11 65.5
MS.gene63113.t1 MTR_8g085430 32.161 199 115 7 20 207 11 200 2.99e-11 65.1
MS.gene63113.t1 MTR_7g084080 34.911 169 94 7 20 182 17 175 4.63e-11 63.9
MS.gene63113.t1 MTR_5g083890 30.653 199 123 6 20 203 6 204 4.70e-11 65.1
MS.gene63113.t1 MTR_4g051480 30.128 156 101 4 20 175 25 172 5.07e-11 65.5
MS.gene63113.t1 MTR_7g033945 30.814 172 99 6 20 182 11 171 5.07e-11 62.0
MS.gene63113.t1 MTR_3g467400 29.825 228 131 10 18 223 1 221 5.63e-11 64.3
MS.gene63113.t1 MTR_4g092300 30.128 156 101 4 20 175 25 172 5.73e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63113.t1 AT4G10400 27.679 448 271 17 20 455 2 408 9.39e-22 97.4
MS.gene63113.t1 AT4G10400 27.679 448 271 17 20 455 2 408 9.39e-22 97.4
MS.gene63113.t1 AT4G10400 27.679 448 271 17 20 455 2 408 9.39e-22 97.4
MS.gene63113.t1 AT1G78750 26.105 475 305 19 4 453 2 455 6.66e-20 92.4
MS.gene63113.t1 AT1G78750 26.105 475 305 19 4 453 2 455 6.66e-20 92.4
MS.gene63113.t1 AT4G00160 27.706 462 284 17 20 461 16 447 2.05e-16 81.6
MS.gene63113.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.39e-15 77.8
MS.gene63113.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.39e-15 77.8
MS.gene63113.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.57e-15 77.4
MS.gene63113.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.57e-15 77.4
MS.gene63113.t1 AT4G09920 32.335 167 103 4 20 184 2 160 1.57e-15 77.4
MS.gene63113.t1 AT5G50270 28.182 440 241 19 20 427 2 398 4.45e-15 77.4
MS.gene63113.t1 AT3G50710 25.758 462 282 14 20 456 2 427 5.63e-15 77.0
MS.gene63113.t1 AT5G53840 25.225 444 243 17 20 426 18 409 1.37e-14 75.9
MS.gene63113.t1 AT5G56440 34.392 189 113 4 20 205 2 182 1.76e-14 75.5
MS.gene63113.t1 AT4G13985 32.836 201 114 9 19 207 18 209 1.94e-13 72.4
MS.gene63113.t1 AT3G59190 28.161 174 108 3 20 184 12 177 3.64e-13 72.0
MS.gene63113.t1 AT3G59190 28.161 174 108 3 20 184 12 177 5.02e-13 70.9
MS.gene63113.t1 AT4G26340 26.856 458 274 17 20 456 2 419 6.65e-13 70.9
MS.gene63113.t1 AT1G48400 29.534 193 122 4 20 205 10 195 1.12e-12 70.1
MS.gene63113.t1 AT1G48400 29.534 193 122 4 20 205 10 195 1.17e-12 70.5
MS.gene63113.t1 AT1G32375 31.795 195 116 6 20 207 2 186 2.31e-12 68.9
MS.gene63113.t1 AT1G32375 31.795 195 116 6 20 207 2 186 2.31e-12 68.9
MS.gene63113.t1 AT3G59200 30.151 199 114 7 20 205 7 193 4.16e-12 67.8
MS.gene63113.t1 AT3G59200 30.151 199 114 7 20 205 7 193 7.12e-12 67.8
MS.gene63113.t1 AT5G60610 25.178 421 256 19 20 420 2 383 8.82e-12 67.0
MS.gene63113.t1 AT5G60610 25.178 421 256 19 20 420 2 383 8.82e-12 67.0
MS.gene63113.t1 AT1G16930 28.641 206 128 7 8 207 3 195 1.09e-11 67.0
MS.gene63113.t1 AT1G16930 28.641 206 128 7 8 207 3 195 1.09e-11 67.0
MS.gene63113.t1 AT1G16930 28.641 206 128 7 8 207 3 195 1.09e-11 67.0
MS.gene63113.t1 AT3G58920 25.615 488 291 19 20 456 2 468 1.89e-11 66.2
MS.gene63113.t1 AT3G52680 31.633 196 115 6 20 208 21 204 2.54e-11 65.9
MS.gene63113.t1 AT3G52680 32.143 196 114 6 20 208 21 204 4.11e-11 64.7
MS.gene63113.t1 AT4G13965 26.681 461 263 18 19 453 9 420 6.26e-11 64.7

Find 110 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATAGTTTACCTTCTATT+TGG 0.229961 8.1:+43181937 MS.gene63113:CDS
CATTGGCAACTTTCACAGTA+TGG 0.259899 8.1:+43182137 MS.gene63113:CDS
AAAATTGTCATTGGCCGATT+TGG 0.299413 8.1:-43182058 None:intergenic
ATAAATCCCGGATCGATTAA+AGG 0.334451 8.1:-43181564 None:intergenic
CAACTTAAATTTGACGTTGA+TGG 0.335217 8.1:+43182932 MS.gene63113:CDS
CTCGCCGGTGGCGCCACCTC+TGG 0.341008 8.1:+43181408 MS.gene63113:CDS
TGATGTAAACGGCTAAATTC+AGG 0.379198 8.1:-43182432 None:intergenic
GGAAGGTGGAATTAAAGCTT+TGG 0.380976 8.1:-43182019 None:intergenic
CTCGGATGAGGTTGAGAATA+GGG 0.388430 8.1:-43182209 None:intergenic
GACGTTGATGGGTCTCATTA+AGG 0.393555 8.1:+43182944 MS.gene63113:CDS
TTTGAATGTCGCGGAATTTG+CGG 0.404825 8.1:-43181523 None:intergenic
CTGACTTATGTTCACATAGA+AGG 0.406124 8.1:+43182746 MS.gene63113:CDS
TGTATCAGATTGGGCATCAT+TGG 0.407705 8.1:+43182120 MS.gene63113:CDS
AACTTAAATTTGACGTTGAT+GGG 0.410278 8.1:+43182933 MS.gene63113:CDS
GTAAAGGAGGTTGGAGCAAT+TGG 0.413248 8.1:-43181708 None:intergenic
TAGATTCACATTCGTTCAAA+AGG 0.416585 8.1:+43181468 MS.gene63113:CDS
AAATAATGAATGTATCAGAT+TGG 0.421870 8.1:+43182110 MS.gene63113:CDS
GAGATAGAGTTGTTGAAGGT+GGG 0.425273 8.1:-43181639 None:intergenic
ACTTGGGACTGCCAAATAGA+AGG 0.427636 8.1:-43181948 None:intergenic
GACTGCAGAAGTTGAATATC+TGG 0.436258 8.1:-43181439 None:intergenic
CGATTTGGAGCTCTCGGAGC+TGG 0.442706 8.1:-43182043 None:intergenic
ACTCTCACCTTTAATCGATC+CGG 0.450492 8.1:+43181557 MS.gene63113:CDS
AATAATGAATGTATCAGATT+GGG 0.455243 8.1:+43182111 MS.gene63113:CDS
CCTTCCAGAGGTGGCGCCAC+CGG 0.463852 8.1:-43181412 None:intergenic
AGTCCAAGTAAAATTGTCAT+TGG 0.468154 8.1:-43182067 None:intergenic
CTCTCACCTTTAATCGATCC+GGG 0.468884 8.1:+43181558 MS.gene63113:CDS
CATCATAGCGACGGAGGTTG+TGG 0.470605 8.1:-43181378 None:intergenic
AACCCGCAAAACTTCCAACT+TGG 0.473471 8.1:-43181965 None:intergenic
CGCGGAATTTGCGGAGGGCG+AGG 0.475468 8.1:-43181514 None:intergenic
GCTCCAACCTCCTTTACCTC+AGG 0.478577 8.1:+43181714 MS.gene63113:CDS
TATACATCATAGCAGTCAGC+TGG 0.478695 8.1:-43182264 None:intergenic
TCTCGGATGAGGTTGAGAAT+AGG 0.481952 8.1:-43182210 None:intergenic
GAAGGTGGAATTAAAGCTTT+GGG 0.484659 8.1:-43182018 None:intergenic
GCAATTATAACCTGAGGTAA+AGG 0.484740 8.1:-43181724 None:intergenic
CATCAACGTCAAATTTAAGT+TGG 0.486323 8.1:-43182931 None:intergenic
TGTAGGTTCTTATAGTATAG+AGG 0.487842 8.1:+43181843 MS.gene63113:CDS
ACGTGGATCCTTGCTGCCAT+TGG 0.491503 8.1:+43181614 MS.gene63113:CDS
GCCATTGAGAACATGGCAGC+TGG 0.493170 8.1:+43182688 MS.gene63113:CDS
ATGTTTCGACTCAAATCTGC+TGG 0.494251 8.1:+43182473 MS.gene63113:CDS
CTTCTATGTGAACATAAGTC+AGG 0.496958 8.1:-43182745 None:intergenic
ATTGGCAACTTTCACAGTAT+GGG 0.502787 8.1:+43182138 MS.gene63113:CDS
TGAACTTCAGATGATGTAAA+CGG 0.503521 8.1:-43182443 None:intergenic
GACAAATTCACTTTCAGTCA+AGG 0.506419 8.1:-43182184 None:intergenic
CAATTGGTCAAGAGGGAAGG+AGG 0.511905 8.1:-43181692 None:intergenic
ATTGGCCGATTTGGAGCTCT+CGG 0.514913 8.1:-43182049 None:intergenic
TTGTTGAAGGTGGGGTCCAA+TGG 0.515637 8.1:-43181630 None:intergenic
TCCAGCTGCCATGTTCTCAA+TGG 0.515812 8.1:-43182689 None:intergenic
TTCTATCATCATCTCGGATG+AGG 0.517274 8.1:-43182221 None:intergenic
GTTCACATAGAAGGATATCA+AGG 0.522561 8.1:+43182755 MS.gene63113:CDS
TAACCTGAGGTAAAGGAGGT+TGG 0.524882 8.1:-43181717 None:intergenic
TGTAGAGATAGAGTTGTTGA+AGG 0.526137 8.1:-43181643 None:intergenic
CAGCTCCGAGAGCTCCAAAT+CGG 0.527982 8.1:+43182044 MS.gene63113:CDS
GAGCTCTCGGAGCTGGAGGA+AGG 0.528347 8.1:-43182036 None:intergenic
AGAGATAGAGTTGTTGAAGG+TGG 0.529187 8.1:-43181640 None:intergenic
AAGATTAACGAATATATCAA+AGG 0.534259 8.1:+43182895 MS.gene63113:CDS
TTGGCAACTTTCACAGTATG+GGG 0.536652 8.1:+43182139 MS.gene63113:CDS
TGATAGTTCAATAGACAAAA+CGG 0.539124 8.1:+43182853 MS.gene63113:CDS
ATGAAAGTTGTCATAAATCC+CGG 0.539720 8.1:-43181576 None:intergenic
GGAGGTTGTGGTAGGGAGGA+AGG 0.546045 8.1:-43181366 None:intergenic
CCGGTGGCGCCACCTCTGGA+AGG 0.547084 8.1:+43181412 MS.gene63113:CDS
AGATTAACGAATATATCAAA+GGG 0.548268 8.1:+43182896 MS.gene63113:CDS
TCTATTTGGCAGTCCCAAGT+TGG 0.550189 8.1:+43181951 MS.gene63113:CDS
TGCCATGTTCTCAATGGCAG+CGG 0.553521 8.1:-43182683 None:intergenic
AGCAGATTTGAGTCGAAACA+TGG 0.553943 8.1:-43182471 None:intergenic
TGTGGCATTGCTCAAGAACA+TGG 0.554101 8.1:-43182497 None:intergenic
CTCTCGGAGCTGGAGGAAGG+TGG 0.556854 8.1:-43182033 None:intergenic
AACCGCTGCCATTGAGAACA+TGG 0.557897 8.1:+43182681 MS.gene63113:CDS
ATATGACACAGGATCGAGTC+CGG 0.559164 8.1:-43181338 None:intergenic
TTCAGGATAATTTAGAACAC+GGG 0.564152 8.1:-43182415 None:intergenic
AATTGGTCAAGAGGGAAGGA+GGG 0.570036 8.1:-43181691 None:intergenic
ACCCGCAAAACTTCCAACTT+GGG 0.570628 8.1:-43181964 None:intergenic
TTCAATAGACAAAACGGACA+TGG 0.570795 8.1:+43182859 MS.gene63113:CDS
GGACAGATTGAGCAGTCTAC+CGG 0.570908 8.1:+43181319 MS.gene63113:CDS
ATAGCGACGGAGGTTGTGGT+AGG 0.574834 8.1:-43181374 None:intergenic
AGATAGAGTTGTTGAAGGTG+GGG 0.580102 8.1:-43181638 None:intergenic
ATTCAGTTCTATCATCATCT+CGG 0.582388 8.1:-43182227 None:intergenic
AGAGACAGCACTTTGAGCAA+TGG 0.588422 8.1:-43181889 None:intergenic
GAATGTCGCGGAATTTGCGG+AGG 0.595538 8.1:-43181520 None:intergenic
GAGCAATTGGTCAAGAGGGA+AGG 0.596231 8.1:-43181695 None:intergenic
GGTTGGAGCAATTGGTCAAG+AGG 0.598188 8.1:-43181700 None:intergenic
ATCTCGCTTGTATTCGTACG+TGG 0.599453 8.1:+43181597 MS.gene63113:CDS
GTTGCGTCATTCAACATCAA+AGG 0.603041 8.1:+43182293 MS.gene63113:CDS
CTATGTGAACATAAGTCAGG+TGG 0.604289 8.1:-43182742 None:intergenic
TAGCGACGGAGGTTGTGGTA+GGG 0.614525 8.1:-43181373 None:intergenic
GATGGCAACGGAGTCAAGCA+TGG 0.616359 8.1:+43181262 None:intergenic
TAATTTAGAACACGGGCATG+AGG 0.618358 8.1:-43182408 None:intergenic
GTTGGAGCAATTGGTCAAGA+GGG 0.618704 8.1:-43181699 None:intergenic
CGACGGAGGTTGTGGTAGGG+AGG 0.621858 8.1:-43181370 None:intergenic
GGTGGGGTCCAATGGCAGCA+AGG 0.624675 8.1:-43181622 None:intergenic
ATTATAACCTGAGGTAAAGG+AGG 0.629004 8.1:-43181721 None:intergenic
ATTCAGGATAATTTAGAACA+CGG 0.629666 8.1:-43182416 None:intergenic
TGATGAGAGTCTCTCGCCGG+TGG 0.630142 8.1:+43181396 MS.gene63113:CDS
TCTGGAGATCCTTCCAGAGG+TGG 0.631588 8.1:-43181421 None:intergenic
TTTGAGCAATGGAAAATGAA+CGG 0.634422 8.1:-43181878 None:intergenic
TTTGGAGCTCTCGGAGCTGG+AGG 0.636682 8.1:-43182040 None:intergenic
TATGTGAACATAAGTCAGGT+GGG 0.640734 8.1:-43182741 None:intergenic
CTATGATGAGAGTCTCTCGC+CGG 0.640993 8.1:+43181393 MS.gene63113:CDS
ATACATCATAGCAGTCAGCT+GGG 0.647711 8.1:-43182263 None:intergenic
AATGTCGCGGAATTTGCGGA+GGG 0.650718 8.1:-43181519 None:intergenic
TCTCATAATTCAGAGCAACA+AGG 0.652411 8.1:+43182530 MS.gene63113:CDS
AACCTAATAGACGATGGCAA+CGG 0.659832 8.1:+43181250 None:intergenic
GGAAGGACATGATATGACAC+AGG 0.667460 8.1:-43181349 None:intergenic
AGAGACTCTCATCATAGCGA+CGG 0.669339 8.1:-43181387 None:intergenic
TGGCAACTTTCACAGTATGG+GGG 0.681502 8.1:+43182140 MS.gene63113:CDS
GACTCTCATCATAGCGACGG+AGG 0.689212 8.1:-43181384 None:intergenic
TTATGAGAACTTGAAGCATG+TGG 0.695487 8.1:-43182515 None:intergenic
TGCAGAAGAAGACAACGTCA+CGG 0.699654 8.1:+43181298 MS.gene63113:CDS
ACTGCTATGATGTATATCTG+CGG 0.701238 8.1:+43182271 MS.gene63113:CDS
ATATCTGGAGATCCTTCCAG+AGG 0.715901 8.1:-43181424 None:intergenic
CATCATAGCAGTCAGCTGGG+AGG 0.736987 8.1:-43182260 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATGAATGTATCAGAT+TGG + chr8.1:43182110-43182129 MS.gene63113:CDS 20.0%
!! AAGATTAACGAATATATCAA+AGG + chr8.1:43182895-43182914 MS.gene63113:CDS 20.0%
!! AATAATGAATGTATCAGATT+GGG + chr8.1:43182111-43182130 MS.gene63113:CDS 20.0%
!! ACATACATACATGTATATAT+AGG - chr8.1:43182598-43182617 None:intergenic 20.0%
!! AGATTAACGAATATATCAAA+GGG + chr8.1:43182896-43182915 MS.gene63113:CDS 20.0%
!! AGTTTAATACAAACAATCTT+TGG + chr8.1:43182568-43182587 MS.gene63113:intron 20.0%
!! ATGGTTTATATGTATATCTA+CGG + chr8.1:43182331-43182350 MS.gene63113:intron 20.0%
!! GTCATTACTCAAAAATATAT+TGG - chr8.1:43182634-43182653 None:intergenic 20.0%
!!! TATATTTTTGAGTAATGACA+TGG + chr8.1:43182635-43182654 MS.gene63113:intron 20.0%
! AAAAGACATACATGTTAGAA+AGG - chr8.1:43181812-43181831 None:intergenic 25.0%
! AACTTAAATTTGACGTTGAT+GGG + chr8.1:43182933-43182952 MS.gene63113:CDS 25.0%
! GAAATAGTTTACCTTCTATT+TGG + chr8.1:43181937-43181956 MS.gene63113:CDS 25.0%
! TAAAAAGCAATTATAACCTG+AGG - chr8.1:43181733-43181752 None:intergenic 25.0%
! TGATAGTTCAATAGACAAAA+CGG + chr8.1:43182853-43182872 MS.gene63113:CDS 25.0%
! TTATATGTATATCTACGGTT+TGG + chr8.1:43182336-43182355 MS.gene63113:intron 25.0%
!! ATTCAGGATAATTTAGAACA+CGG - chr8.1:43182419-43182438 None:intergenic 25.0%
!!! AAGGTGATGTTTTATTCTTA+TGG + chr8.1:43182312-43182331 MS.gene63113:intron 25.0%
!!! CATTTGCTGAATATATTTTG+CGG + chr8.1:43182795-43182814 MS.gene63113:CDS 25.0%
AGTCCAAGTAAAATTGTCAT+TGG - chr8.1:43182070-43182089 None:intergenic 30.0%
ATGAAAGTTGTCATAAATCC+CGG - chr8.1:43181579-43181598 None:intergenic 30.0%
ATTCAGTTCTATCATCATCT+CGG - chr8.1:43182230-43182249 None:intergenic 30.0%
CAACTTAAATTTGACGTTGA+TGG + chr8.1:43182932-43182951 MS.gene63113:CDS 30.0%
CATCAACGTCAAATTTAAGT+TGG - chr8.1:43182934-43182953 None:intergenic 30.0%
GAAATGTTAGAAGTGAGTTT+GGG - chr8.1:43181758-43181777 None:intergenic 30.0%
TAGATTCACATTCGTTCAAA+AGG + chr8.1:43181468-43181487 MS.gene63113:CDS 30.0%
TGAAATGTTAGAAGTGAGTT+TGG - chr8.1:43181759-43181778 None:intergenic 30.0%
TGAACTTCAGATGATGTAAA+CGG - chr8.1:43182446-43182465 None:intergenic 30.0%
TGTAGGTTCTTATAGTATAG+AGG + chr8.1:43181843-43181862 MS.gene63113:CDS 30.0%
TTTGAGCAATGGAAAATGAA+CGG - chr8.1:43181881-43181900 None:intergenic 30.0%
! TTCAGGATAATTTAGAACAC+GGG - chr8.1:43182418-43182437 None:intergenic 30.0%
! TTTTAGACTCACAATTTCTG+CGG - chr8.1:43181917-43181936 None:intergenic 30.0%
!! TCTTTTGAAAACAGTCTTGT+AGG + chr8.1:43181826-43181845 MS.gene63113:intron 30.0%
AAAATTGTCATTGGCCGATT+TGG - chr8.1:43182061-43182080 None:intergenic 35.0%
ACTGCTATGATGTATATCTG+CGG + chr8.1:43182271-43182290 MS.gene63113:CDS 35.0%
ATAAATCCCGGATCGATTAA+AGG - chr8.1:43181567-43181586 None:intergenic 35.0%
ATTATAACCTGAGGTAAAGG+AGG - chr8.1:43181724-43181743 None:intergenic 35.0%
ATTGGCAACTTTCACAGTAT+GGG + chr8.1:43182138-43182157 MS.gene63113:CDS 35.0%
CTGACTTATGTTCACATAGA+AGG + chr8.1:43182746-43182765 MS.gene63113:CDS 35.0%
CTTCTATGTGAACATAAGTC+AGG - chr8.1:43182748-43182767 None:intergenic 35.0%
GAAGGTGGAATTAAAGCTTT+GGG - chr8.1:43182021-43182040 None:intergenic 35.0%
GACAAATTCACTTTCAGTCA+AGG - chr8.1:43182187-43182206 None:intergenic 35.0%
GCAATTATAACCTGAGGTAA+AGG - chr8.1:43181727-43181746 None:intergenic 35.0%
GTTCACATAGAAGGATATCA+AGG + chr8.1:43182755-43182774 MS.gene63113:CDS 35.0%
TATGTGAACATAAGTCAGGT+GGG - chr8.1:43182744-43182763 None:intergenic 35.0%
TCTCATAATTCAGAGCAACA+AGG + chr8.1:43182530-43182549 MS.gene63113:CDS 35.0%
TGATGTAAACGGCTAAATTC+AGG - chr8.1:43182435-43182454 None:intergenic 35.0%
TGTAGAGATAGAGTTGTTGA+AGG - chr8.1:43181646-43181665 None:intergenic 35.0%
TTATGAGAACTTGAAGCATG+TGG - chr8.1:43182518-43182537 None:intergenic 35.0%
TTCAATAGACAAAACGGACA+TGG + chr8.1:43182859-43182878 MS.gene63113:CDS 35.0%
! GATGAAACTTTTGAATGTCG+CGG - chr8.1:43181535-43181554 None:intergenic 35.0%
! GCTGAATATATTTTGCGGAA+TGG + chr8.1:43182800-43182819 MS.gene63113:CDS 35.0%
AATGACATGGTTTCCCTTGT+TGG + chr8.1:43182648-43182667 MS.gene63113:intron 40.0%
ACTCTCACCTTTAATCGATC+CGG + chr8.1:43181557-43181576 MS.gene63113:CDS 40.0%
AGAGATAGAGTTGTTGAAGG+TGG - chr8.1:43181643-43181662 None:intergenic 40.0%
AGATAGAGTTGTTGAAGGTG+GGG - chr8.1:43181641-43181660 None:intergenic 40.0%
AGCAGATTTGAGTCGAAACA+TGG - chr8.1:43182474-43182493 None:intergenic 40.0%
ATACATCATAGCAGTCAGCT+GGG - chr8.1:43182266-43182285 None:intergenic 40.0%
ATGTTTCGACTCAAATCTGC+TGG + chr8.1:43182473-43182492 MS.gene63113:CDS 40.0%
CATTGGCAACTTTCACAGTA+TGG + chr8.1:43182137-43182156 MS.gene63113:CDS 40.0%
CTATGTGAACATAAGTCAGG+TGG - chr8.1:43182745-43182764 None:intergenic 40.0%
GACTGCAGAAGTTGAATATC+TGG - chr8.1:43181442-43181461 None:intergenic 40.0%
GAGATAGAGTTGTTGAAGGT+GGG - chr8.1:43181642-43181661 None:intergenic 40.0%
GGAAGGTGGAATTAAAGCTT+TGG - chr8.1:43182022-43182041 None:intergenic 40.0%
GTTGCGTCATTCAACATCAA+AGG + chr8.1:43182293-43182312 MS.gene63113:CDS 40.0%
TATACATCATAGCAGTCAGC+TGG - chr8.1:43182267-43182286 None:intergenic 40.0%
TGTATCAGATTGGGCATCAT+TGG + chr8.1:43182120-43182139 MS.gene63113:CDS 40.0%
TTCTATCATCATCTCGGATG+AGG - chr8.1:43182224-43182243 None:intergenic 40.0%
TTGGCAACTTTCACAGTATG+GGG + chr8.1:43182139-43182158 MS.gene63113:CDS 40.0%
TTTGAATGTCGCGGAATTTG+CGG - chr8.1:43181526-43181545 None:intergenic 40.0%
! CGGCCAATGACAATTTTACT+TGG + chr8.1:43182064-43182083 MS.gene63113:CDS 40.0%
! TAATTTAGAACACGGGCATG+AGG - chr8.1:43182411-43182430 None:intergenic 40.0%
AACCCGCAAAACTTCCAACT+TGG - chr8.1:43181968-43181987 None:intergenic 45.0%
AATTGGTCAAGAGGGAAGGA+GGG - chr8.1:43181694-43181713 None:intergenic 45.0%
ACCCGCAAAACTTCCAACTT+GGG - chr8.1:43181967-43181986 None:intergenic 45.0%
ACTTGGGACTGCCAAATAGA+AGG - chr8.1:43181951-43181970 None:intergenic 45.0%
AGAGACAGCACTTTGAGCAA+TGG - chr8.1:43181892-43181911 None:intergenic 45.0%
AGAGACTCTCATCATAGCGA+CGG - chr8.1:43181390-43181409 None:intergenic 45.0%
ATATCTGGAGATCCTTCCAG+AGG - chr8.1:43181427-43181446 None:intergenic 45.0%
ATATGACACAGGATCGAGTC+CGG - chr8.1:43181341-43181360 None:intergenic 45.0%
ATCTCGCTTGTATTCGTACG+TGG + chr8.1:43181597-43181616 MS.gene63113:CDS 45.0%
CTCGGATGAGGTTGAGAATA+GGG - chr8.1:43182212-43182231 None:intergenic 45.0%
CTCTCACCTTTAATCGATCC+GGG + chr8.1:43181558-43181577 MS.gene63113:CDS 45.0%
GGAAGGACATGATATGACAC+AGG - chr8.1:43181352-43181371 None:intergenic 45.0%
GTAAAGGAGGTTGGAGCAAT+TGG - chr8.1:43181711-43181730 None:intergenic 45.0%
GTTGGAGCAATTGGTCAAGA+GGG - chr8.1:43181702-43181721 None:intergenic 45.0%
TAACCTGAGGTAAAGGAGGT+TGG - chr8.1:43181720-43181739 None:intergenic 45.0%
TCTATTTGGCAGTCCCAAGT+TGG + chr8.1:43181951-43181970 MS.gene63113:CDS 45.0%
TCTCGGATGAGGTTGAGAAT+AGG - chr8.1:43182213-43182232 None:intergenic 45.0%
TGCAGAAGAAGACAACGTCA+CGG + chr8.1:43181298-43181317 MS.gene63113:CDS 45.0%
TGGCAACTTTCACAGTATGG+GGG + chr8.1:43182140-43182159 MS.gene63113:CDS 45.0%
TGTGGCATTGCTCAAGAACA+TGG - chr8.1:43182500-43182519 None:intergenic 45.0%
TTGTTATCGTGTACCTGCAG+TGG + chr8.1:43182382-43182401 MS.gene63113:intron 45.0%
!! GTCCCAAGTTGGAAGTTTTG+CGG + chr8.1:43181962-43181981 MS.gene63113:CDS 45.0%
!! TCCCAAGTTGGAAGTTTTGC+GGG + chr8.1:43181963-43181982 MS.gene63113:CDS 45.0%
AACCGCTGCCATTGAGAACA+TGG + chr8.1:43182681-43182700 MS.gene63113:CDS 50.0%
AATGTCGCGGAATTTGCGGA+GGG - chr8.1:43181522-43181541 None:intergenic 50.0%
CAATTGGTCAAGAGGGAAGG+AGG - chr8.1:43181695-43181714 None:intergenic 50.0%
CTATGATGAGAGTCTCTCGC+CGG + chr8.1:43181393-43181412 MS.gene63113:CDS 50.0%
GACATGGTTTCCCTTGTTGG+TGG + chr8.1:43182651-43182670 MS.gene63113:intron 50.0%
GAGCAATTGGTCAAGAGGGA+AGG - chr8.1:43181698-43181717 None:intergenic 50.0%
GGACAGATTGAGCAGTCTAC+CGG + chr8.1:43181319-43181338 MS.gene63113:CDS 50.0%
GGTTGGAGCAATTGGTCAAG+AGG - chr8.1:43181703-43181722 None:intergenic 50.0%
GTTCCTCCTACCACCAACAA+GGG - chr8.1:43182664-43182683 None:intergenic 50.0%
TCCAGCTGCCATGTTCTCAA+TGG - chr8.1:43182692-43182711 None:intergenic 50.0%
TGCCATGTTCTCAATGGCAG+CGG - chr8.1:43182686-43182705 None:intergenic 50.0%
TTGTTGAAGGTGGGGTCCAA+TGG - chr8.1:43181633-43181652 None:intergenic 50.0%
!! ATTGGCCGATTTGGAGCTCT+CGG - chr8.1:43182052-43182071 None:intergenic 50.0%
!! TGGTTTCCCTTGTTGGTGGT+AGG + chr8.1:43182655-43182674 MS.gene63113:intron 50.0%
!! TTTCCCTTGTTGGTGGTAGG+AGG + chr8.1:43182658-43182677 MS.gene63113:intron 50.0%
ACGTGGATCCTTGCTGCCAT+TGG + chr8.1:43181614-43181633 MS.gene63113:CDS 55.0%
ATAGCGACGGAGGTTGTGGT+AGG - chr8.1:43181377-43181396 None:intergenic 55.0%
CAGCTCCGAGAGCTCCAAAT+CGG + chr8.1:43182044-43182063 MS.gene63113:CDS 55.0%
CATCATAGCAGTCAGCTGGG+AGG - chr8.1:43182263-43182282 None:intergenic 55.0%
CATCATAGCGACGGAGGTTG+TGG - chr8.1:43181381-43181400 None:intergenic 55.0%
GAATGTCGCGGAATTTGCGG+AGG - chr8.1:43181523-43181542 None:intergenic 55.0%
GACTCTCATCATAGCGACGG+AGG - chr8.1:43181387-43181406 None:intergenic 55.0%
GCCATTGAGAACATGGCAGC+TGG + chr8.1:43182688-43182707 MS.gene63113:CDS 55.0%
GCTCCAACCTCCTTTACCTC+AGG + chr8.1:43181714-43181733 MS.gene63113:CDS 55.0%
GGTTCCTCCTACCACCAACA+AGG - chr8.1:43182665-43182684 None:intergenic 55.0%
TAGCGACGGAGGTTGTGGTA+GGG - chr8.1:43181376-43181395 None:intergenic 55.0%
TCTGGAGATCCTTCCAGAGG+TGG - chr8.1:43181424-43181443 None:intergenic 55.0%
GGAGGTTGTGGTAGGGAGGA+AGG - chr8.1:43181369-43181388 None:intergenic 60.0%
TGATGAGAGTCTCTCGCCGG+TGG + chr8.1:43181396-43181415 MS.gene63113:CDS 60.0%
! CGATTTGGAGCTCTCGGAGC+TGG - chr8.1:43182046-43182065 None:intergenic 60.0%
! TTTGGAGCTCTCGGAGCTGG+AGG - chr8.1:43182043-43182062 None:intergenic 60.0%
CGACGGAGGTTGTGGTAGGG+AGG - chr8.1:43181373-43181392 None:intergenic 65.0%
CTCTCGGAGCTGGAGGAAGG+TGG - chr8.1:43182036-43182055 None:intergenic 65.0%
GAGCTCTCGGAGCTGGAGGA+AGG - chr8.1:43182039-43182058 None:intergenic 65.0%
GGTGGGGTCCAATGGCAGCA+AGG - chr8.1:43181625-43181644 None:intergenic 65.0%
CCTTCCAGAGGTGGCGCCAC+CGG - chr8.1:43181415-43181434 None:intergenic 70.0%
CGCGGAATTTGCGGAGGGCG+AGG - chr8.1:43181517-43181536 None:intergenic 70.0%
GGGCATGAGGTCGCCACTGC+AGG - chr8.1:43182398-43182417 None:intergenic 70.0%
CCGGTGGCGCCACCTCTGGA+AGG + chr8.1:43181412-43181431 MS.gene63113:CDS 75.0%
! CTCGCCGGTGGCGCCACCTC+TGG + chr8.1:43181408-43181427 MS.gene63113:CDS 80.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 43181263 43182964 43181263 ID=MS.gene63113
chr8.1 mRNA 43181263 43182964 43181263 ID=MS.gene63113.t1;Parent=MS.gene63113
chr8.1 exon 43181263 43181735 43181263 ID=MS.gene63113.t1.exon1;Parent=MS.gene63113.t1
chr8.1 CDS 43181263 43181735 43181263 ID=cds.MS.gene63113.t1;Parent=MS.gene63113.t1
chr8.1 exon 43181840 43182314 43181840 ID=MS.gene63113.t1.exon2;Parent=MS.gene63113.t1
chr8.1 CDS 43181840 43182314 43181840 ID=cds.MS.gene63113.t1;Parent=MS.gene63113.t1
chr8.1 exon 43182402 43182551 43182402 ID=MS.gene63113.t1.exon3;Parent=MS.gene63113.t1
chr8.1 CDS 43182402 43182551 43182402 ID=cds.MS.gene63113.t1;Parent=MS.gene63113.t1
chr8.1 exon 43182677 43182964 43182677 ID=MS.gene63113.t1.exon4;Parent=MS.gene63113.t1
chr8.1 CDS 43182677 43182964 43182677 ID=cds.MS.gene63113.t1;Parent=MS.gene63113.t1
Gene Sequence

>MS.gene63113

ATGGCAACGGAGTCAAGCATGGAACGATCGCTTGTTGCAGAAGAAGACAACGTCACGGACAGATTGAGCAGTCTACCGGACTCGATCCTGTGTCATATCATGTCCTTCCTCCCTACCACAACCTCCGTCGCTATGATGAGAGTCTCTCGCCGGTGGCGCCACCTCTGGAAGGATCTCCAGATATTCAACTTCTGCAGTCGCAATCTAGATTCACATTCGTTCAAAAGGTTTTTATTATTCGTGAACGCTGTCCTCGCCCTCCGCAAATTCCGCGACATTCAAAAGTTTCATCTCACTCTCACCTTTAATCGATCCGGGATTTATGACAACTTTCATCTCGCTTGTATTCGTACGTGGATCCTTGCTGCCATTGGACCCCACCTTCAACAACTCTATCTCTACATTGTCAATGCTGCTGTTGTAGTTCTCCCTCCTTCCCTCTTGACCAATTGCTCCAACCTCCTTTACCTCAGGTTATAATTGCTTTTTATGCCCAAACTCACTTCTAACATTTCATCTCGTTTTTTTTTTTTACTTTTTTTTTTTCCTTTCTAACATGTATGTCTTTTGAAAACAGTCTTGTAGGTTCTTATAGTATAGAGGTAAAACAGTCTTCCGTTCATTTTCCATTGCTCAAAGTGCTGTCTCTCGCCGCAGAAATTGTGAGTCTAAAAGAAATAGTTTACCTTCTATTTGGCAGTCCCAAGTTGGAAGTTTTGCGGGTTTACTTTTACTTTATCTCTGAAGAACTTGTGCCCAAAGCTTTAATTCCACCTTCCTCCAGCTCCGAGAGCTCCAAATCGGCCAATGACAATTTTACTTGGACTTACTTTAAAATAGTTAACACAAATAATGAATGTATCAGATTGGGCATCATTGGCAACTTTCACAGTATGGGGGAAGCATTTCTTGATGTCTTTTCCTTGACTGAAAGTGAATTTGTCGACCCTATTCTCAACCTCATCCGAGATGATGATAGAACTGAATTTTATAAAAACCTCCCAGCTGACTGCTATGATGTATATCTGCGGTTGCGTCATTCAACATCAAAGGTGATGTTTTATTCTTATGGTTTATATGTATATCTACGGTTTGGTACTAATAGTGCGTCATCACTTGTTGTTATCGTGTACCTGCAGTGGCGACCTCATGCCCGTGTTCTAAATTATCCTGAATTTAGCCGTTTACATCATCTGAAGTTCATTCTTCCATGTTTCGACTCAAATCTGCTGGTCCATGTTCTTGAGCAATGCCACATGCTTCAAGTTCTCATAATTCAGAGCAACAAGGTTCGTTGACTTCTCAAGTTTAATACAAACAATCTTTGGTCTGCCTATATATACATGTATGTATGTATCTATCTGTACACCAATATATTTTTGAGTAATGACATGGTTTCCCTTGTTGGTGGTAGGAGGAACCGCTGCCATTGAGAACATGGCAGCTGGAGTCAACAACAGTTTTTGAATGTCTAAAATCCCACCTGACTTATGTTCACATAGAAGGATATCAAGGACTTGAAGATGAGTTCACATTTGCTGAATATATTTTGCGGAATGGACTTCTTTTGAAGACGATGCTTATTTTCGTTGATAGTTCAATAGACAAAACGGACATGGATTGTTCTCTCAAAAGATTAACGAATATATCAAAGGGCTCTGTTGCTTGCCAACTTAAATTTGACGTTGATGGGTCTCATTAA
Protein sequence

>MS.gene63113.t1

MATESSMERSLVAEEDNVTDRLSSLPDSILCHIMSFLPTTTSVAMMRVSRRWRHLWKDLQIFNFCSRNLDSHSFKRFLLFVNAVLALRKFRDIQKFHLTLTFNRSGIYDNFHLACIRTWILAAIGPHLQQLYLYIVNAAVVVLPPSLLTNCSNLLYLSLVGSYSIEVKQSSVHFPLLKVLSLAAEIVSLKEIVYLLFGSPKLEVLRVYFYFISEELVPKALIPPSSSSESSKSANDNFTWTYFKIVNTNNECIRLGIIGNFHSMGEAFLDVFSLTESEFVDPILNLIRDDDRTEFYKNLPADCYDVYLRLRHSTSKWRPHARVLNYPEFSRLHHLKFILPCFDSNLLVHVLEQCHMLQVLIIQSNKEEPLPLRTWQLESTTVFECLKSHLTYVHIEGYQGLEDEFTFAEYILRNGLLLKTMLIFVDSSIDKTDMDCSLKRLTNISKGSVACQLKFDVDGSH