AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63177

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63177.t1 MTR_8g062300 57.287 494 158 10 1 453 1 482 2.35e-175 500
MS.gene63177.t1 MTR_8g070950 57.234 470 151 11 1 452 1 438 8.28e-165 472
MS.gene63177.t1 MTR_8g062080 55.940 463 181 9 1 453 1 450 9.86e-162 464
MS.gene63177.t1 MTR_8g062010 56.152 447 158 10 23 452 9 434 1.68e-152 440
MS.gene63177.t1 MTR_8g062310 53.229 449 184 7 1 448 5 428 3.43e-152 439
MS.gene63177.t1 MTR_8g469020 50.638 470 173 13 22 452 6 455 1.80e-139 408
MS.gene63177.t1 MTR_8g058965 49.380 484 182 12 9 453 2 461 2.54e-133 392
MS.gene63177.t1 MTR_4g050250 48.533 443 156 13 1 394 1 420 5.25e-114 342
MS.gene63177.t1 MTR_8g467790 46.078 408 141 12 23 402 148 504 4.12e-101 311
MS.gene63177.t1 MTR_8g468810 39.175 485 236 16 1 448 1 463 7.50e-87 273
MS.gene63177.t1 MTR_8g062270 37.581 463 232 14 20 447 13 453 2.88e-79 253
MS.gene63177.t1 MTR_2g083840 38.950 457 230 12 22 448 20 457 1.46e-78 251
MS.gene63177.t1 MTR_4g032730 35.845 491 237 15 9 447 2 466 1.74e-74 241
MS.gene63177.t1 MTR_2g086020 82.759 145 25 0 308 452 175 319 6.27e-74 235
MS.gene63177.t1 MTR_2g086020 59.091 154 55 2 49 198 1 150 5.90e-49 170
MS.gene63177.t1 MTR_8g058970 36.128 501 240 19 3 448 2 477 2.36e-71 233
MS.gene63177.t1 MTR_8g467800 37.447 470 241 17 8 448 8 453 7.65e-70 229
MS.gene63177.t1 MTR_8g014260 38.242 455 233 19 23 448 16 451 1.10e-69 228
MS.gene63177.t1 MTR_8g468840 36.325 468 225 15 21 446 12 448 1.02e-68 226
MS.gene63177.t1 MTR_8g468810 39.440 393 181 15 1 358 1 371 2.80e-63 209
MS.gene63177.t1 MTR_5g092960 33.684 475 257 13 11 447 7 461 8.26e-62 208
MS.gene63177.t1 MTR_8g468760 35.575 461 230 17 10 447 2 418 2.55e-60 202
MS.gene63177.t1 MTR_8g445780 35.765 425 214 16 1 401 1 390 5.42e-55 188
MS.gene63177.t1 MTR_8g468810 42.122 311 138 12 1 281 1 299 9.11e-55 185
MS.gene63177.t1 MTR_8g468810 42.122 311 138 12 1 281 1 299 1.92e-54 185
MS.gene63177.t1 MTR_8g468810 42.122 311 138 12 1 281 1 299 1.96e-54 185
MS.gene63177.t1 MTR_5g092960 33.246 382 204 11 11 358 7 371 1.01e-39 147
MS.gene63177.t1 MTR_4g024710 34.884 301 161 11 166 448 64 347 5.20e-37 139
MS.gene63177.t1 MTR_8g467800 38.305 295 152 13 8 281 8 293 5.04e-34 130
MS.gene63177.t1 MTR_8g014210 41.117 197 100 6 253 448 50 231 3.87e-32 122
MS.gene63177.t1 MTR_8g077460 45.638 149 77 4 301 448 230 375 7.81e-32 125
MS.gene63177.t1 MTR_8g077460 35.909 220 119 8 23 229 10 220 6.87e-24 103
MS.gene63177.t1 MTR_3g099800 72.840 81 21 1 360 439 19 99 1.21e-31 117
MS.gene63177.t1 MTR_8g014160 34.164 281 147 10 14 265 32 303 1.53e-29 120
MS.gene63177.t1 MTR_8g014160 50.538 93 43 3 356 448 336 425 2.85e-18 87.0
MS.gene63177.t1 MTR_7g098510 27.752 436 243 14 23 448 18 391 1.06e-28 117
MS.gene63177.t1 MTR_1g041245 29.054 444 231 18 22 448 10 386 2.76e-27 112
MS.gene63177.t1 MTR_5g021550 44.079 152 71 7 17 156 13 162 1.61e-25 102
MS.gene63177.t1 MTR_7g032380 28.146 437 212 17 23 448 11 356 2.03e-25 107
MS.gene63177.t1 MTR_5g008950 26.651 439 233 18 25 448 1 365 1.93e-23 101
MS.gene63177.t1 MTR_4g024745 32.394 284 157 11 166 431 18 284 2.71e-23 99.8
MS.gene63177.t1 MTR_3g107160 25.618 445 257 15 15 448 2 383 7.16e-22 97.4
MS.gene63177.t1 MTR_8g014290 36.601 153 77 5 23 157 10 160 5.73e-21 89.7
MS.gene63177.t1 MTR_7g098480 27.765 443 232 18 22 447 17 388 8.95e-20 92.0
MS.gene63177.t1 MTR_5g080710 27.069 447 264 17 23 440 33 446 4.85e-19 89.7
MS.gene63177.t1 MTR_7g026500 25.282 443 251 15 23 447 14 394 5.68e-19 89.0
MS.gene63177.t1 MTR_7g098570 26.606 436 242 16 22 444 19 389 8.30e-19 89.0
MS.gene63177.t1 MTR_7g033990 26.773 437 229 17 23 440 11 375 9.01e-19 88.6
MS.gene63177.t1 MTR_2g007450 27.837 467 242 22 16 445 9 417 4.61e-18 86.3
MS.gene63177.t1 MTR_5g084070 26.087 437 282 14 19 440 9 419 3.59e-17 84.0
MS.gene63177.t1 MTR_7g056447 32.189 233 128 10 23 248 9 218 2.92e-16 79.3
MS.gene63177.t1 MTR_7g056430 25.995 427 229 17 23 440 9 357 3.50e-16 82.0
MS.gene63177.t1 MTR_7g056420 25.995 427 229 17 23 440 9 357 4.41e-16 81.6
MS.gene63177.t1 MTR_4g024720 36.111 180 71 9 8 156 10 176 1.32e-15 75.9
MS.gene63177.t1 MTR_3g036250 23.953 430 251 14 22 445 4 363 1.99e-15 77.8
MS.gene63177.t1 MTR_5g008920 25.740 439 247 18 23 448 9 381 2.73e-15 77.8
MS.gene63177.t1 MTR_7g115270 31.818 220 114 11 23 219 12 218 6.49e-15 76.6
MS.gene63177.t1 MTR_6g040270 24.711 433 250 19 25 445 10 378 4.76e-14 73.9
MS.gene63177.t1 MTR_3g040700 24.112 394 222 13 22 407 42 366 5.12e-14 74.3
MS.gene63177.t1 MTR_1g053025 26.077 441 252 21 23 445 2 386 1.85e-13 72.0
MS.gene63177.t1 MTR_6g022360 24.598 435 256 17 19 447 39 407 2.02e-13 72.0
MS.gene63177.t1 MTR_3g052990 33.702 181 114 4 6 186 11 185 2.75e-13 72.0
MS.gene63177.t1 MTR_6g011460 24.783 460 256 19 9 448 32 421 3.66e-13 71.2
MS.gene63177.t1 MTR_3g107100 27.358 212 134 8 19 222 7 206 5.34e-13 70.9
MS.gene63177.t1 MTR_3g052940 33.149 181 115 4 6 186 11 185 7.35e-13 70.5
MS.gene63177.t1 MTR_3g052940 32.597 181 116 4 6 186 11 185 8.08e-13 70.5
MS.gene63177.t1 MTR_8g085430 32.512 203 107 9 22 208 10 198 8.16e-13 69.7
MS.gene63177.t1 MTR_3g052940 32.597 181 116 4 6 186 11 185 8.17e-13 70.5
MS.gene63177.t1 MTR_4g098590 25.366 410 231 15 25 415 1 354 1.62e-12 68.9
MS.gene63177.t1 MTR_3g107120 26.398 447 238 20 23 445 9 388 1.94e-12 68.9
MS.gene63177.t1 MTR_3g105060 28.846 208 136 7 23 227 14 212 3.34e-12 68.2
MS.gene63177.t1 MTR_1g038710 25.629 437 257 19 23 417 19 429 5.64e-12 67.8
MS.gene63177.t1 MTR_4g093690 29.323 266 162 13 19 274 14 263 8.25e-12 65.9
MS.gene63177.t1 MTR_5g083890 24.775 444 238 17 23 430 6 389 9.38e-12 67.4
MS.gene63177.t1 MTR_5g073420 29.381 194 122 8 23 209 11 196 2.32e-11 65.5
MS.gene63177.t1 MTR_8g014230 39.506 81 47 2 358 438 17 95 2.54e-11 60.8
MS.gene63177.t1 MTR_2g007500 31.188 202 95 8 22 208 13 185 3.50e-11 64.7
MS.gene63177.t1 MTR_3g082890 32.995 197 108 9 23 210 97 278 4.87e-11 65.1
MS.gene63177.t1 MTR_7g007080 28.125 448 222 25 23 445 4 376 6.04e-11 64.3
MS.gene63177.t1 MTR_0009s0080 24.691 405 228 16 28 415 23 367 8.47e-11 63.9
MS.gene63177.t1 MTR_7g034110 42.222 90 46 3 23 109 11 97 9.26e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63177.t1 AT5G53635 27.033 455 269 19 25 447 2 425 5.11e-21 95.5
MS.gene63177.t1 AT1G32375 28.540 452 266 20 23 448 2 422 9.33e-21 94.7
MS.gene63177.t1 AT1G32375 28.540 452 266 20 23 448 2 422 9.33e-21 94.7
MS.gene63177.t1 AT1G78750 27.122 483 274 25 13 445 1 455 2.84e-20 93.6
MS.gene63177.t1 AT1G78750 27.122 483 274 25 13 445 1 455 2.84e-20 93.6
MS.gene63177.t1 AT4G26340 28.228 457 258 18 23 448 2 419 1.43e-18 87.8
MS.gene63177.t1 AT1G13780 26.078 464 281 21 23 445 10 452 4.43e-18 86.7
MS.gene63177.t1 AT5G56420 25.439 456 271 17 23 448 6 422 5.16e-18 86.3
MS.gene63177.t1 AT5G56420 25.439 456 271 17 23 448 6 422 5.16e-18 86.3
MS.gene63177.t1 AT5G56420 25.439 456 271 17 23 448 6 422 5.16e-18 86.3
MS.gene63177.t1 AT4G10400 26.211 454 259 16 23 447 2 408 3.17e-17 84.0
MS.gene63177.t1 AT4G10400 26.211 454 259 16 23 447 2 408 3.17e-17 84.0
MS.gene63177.t1 AT4G10400 26.211 454 259 16 23 447 2 408 3.17e-17 84.0
MS.gene63177.t1 AT1G51370 26.164 451 264 20 23 443 19 430 6.28e-16 80.1
MS.gene63177.t1 AT5G56400 24.892 462 274 16 22 448 32 455 1.10e-15 79.3
MS.gene63177.t1 AT4G13985 25.514 486 277 23 10 445 6 456 1.12e-15 79.3
MS.gene63177.t1 AT1G78760 25.316 474 288 20 13 449 1 445 1.66e-15 79.0
MS.gene63177.t1 AT1G66290 25.424 472 280 20 9 445 16 450 3.31e-15 77.8
MS.gene63177.t1 AT4G09920 32.474 194 114 7 23 210 2 184 9.07e-15 75.1
MS.gene63177.t1 AT4G09920 32.474 194 114 7 23 210 2 184 9.07e-15 75.1
MS.gene63177.t1 AT4G09920 32.474 194 114 7 23 210 2 184 9.07e-15 75.1
MS.gene63177.t1 AT5G56440 25.862 464 269 15 23 447 2 429 1.04e-14 76.3
MS.gene63177.t1 AT5G50270 26.526 426 247 13 23 415 2 394 1.14e-14 75.9
MS.gene63177.t1 AT4G09920 32.474 194 114 7 23 210 2 184 1.35e-14 75.1
MS.gene63177.t1 AT4G09920 32.474 194 114 7 23 210 2 184 1.35e-14 75.1
MS.gene63177.t1 AT3G59200 33.028 218 120 11 23 227 7 211 1.57e-14 75.1
MS.gene63177.t1 AT5G18770 26.190 462 278 17 23 446 24 460 2.17e-14 75.5
MS.gene63177.t1 AT3G59200 33.028 218 120 11 23 227 7 211 2.83e-14 75.5
MS.gene63177.t1 AT5G22670 25.822 426 249 19 23 411 11 406 3.94e-14 74.7
MS.gene63177.t1 AT1G69630 26.371 474 270 21 10 440 6 443 4.43e-14 74.3
MS.gene63177.t1 AT5G22660 26.013 469 263 21 23 445 13 443 5.28e-14 74.3
MS.gene63177.t1 AT2G26030 25.000 464 283 18 23 445 4 443 5.46e-14 74.3
MS.gene63177.t1 AT2G26030 25.431 464 276 19 23 445 4 438 1.00e-13 73.2
MS.gene63177.t1 AT2G26030 25.431 464 276 19 23 445 4 438 1.00e-13 73.2
MS.gene63177.t1 AT3G58940 31.754 211 108 9 23 210 2 199 1.37e-13 73.6
MS.gene63177.t1 AT3G58940 31.754 211 108 9 23 210 2 199 1.37e-13 73.6
MS.gene63177.t1 AT1G66320 25.577 477 273 21 11 445 18 454 1.74e-13 72.8
MS.gene63177.t1 AT5G44950 25.164 457 272 19 23 439 4 430 8.55e-13 70.5
MS.gene63177.t1 AT5G44960 28.337 427 222 21 23 413 5 383 2.67e-12 68.9
MS.gene63177.t1 AT1G13780 30.455 220 119 11 23 224 10 213 3.10e-12 68.2
MS.gene63177.t1 AT3G26930 27.097 465 268 20 23 453 21 448 1.03e-11 67.0
MS.gene63177.t1 AT4G15060 29.630 216 125 10 7 210 11 211 1.53e-11 66.6
MS.gene63177.t1 AT5G53640 30.144 209 130 8 8 213 6 201 1.62e-11 64.3
MS.gene63177.t1 AT1G66310 25.053 471 283 19 9 445 5 439 1.97e-11 66.2
MS.gene63177.t1 AT3G26922 26.792 265 172 10 14 274 7 253 3.12e-11 64.7
MS.gene63177.t1 AT3G26922 26.792 265 172 10 14 274 7 253 3.12e-11 64.7
MS.gene63177.t1 AT5G22700 31.795 195 110 7 23 210 6 184 3.44e-11 65.5
MS.gene63177.t1 AT5G22700 31.795 195 110 7 23 210 6 184 3.44e-11 65.5
MS.gene63177.t1 AT5G22700 31.795 195 110 7 23 210 6 184 3.44e-11 65.5
MS.gene63177.t1 AT5G22700 31.795 195 110 7 23 210 6 184 3.44e-11 65.5
MS.gene63177.t1 AT5G22700 31.795 195 110 7 23 210 43 221 4.20e-11 65.1
MS.gene63177.t1 AT5G22700 31.795 195 110 7 23 210 43 221 4.22e-11 65.1
MS.gene63177.t1 AT5G41840 29.577 213 107 9 23 208 14 210 4.67e-11 65.5
MS.gene63177.t1 AT4G00315 32.308 195 117 8 23 210 2 188 5.97e-11 64.7
MS.gene63177.t1 AT5G35995 29.707 239 133 9 23 254 5 215 7.39e-11 64.3
MS.gene63177.t1 AT5G35995 29.707 239 133 9 23 254 5 215 7.39e-11 64.3
MS.gene63177.t1 AT1G51370 30.412 194 112 8 23 211 19 194 8.36e-11 63.5

Find 104 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTCAACTTCGTTCAAAA+AGG 0.237370 8.4:-40849881 MS.gene63177:CDS
TCGATTTCTTCTTCTGCTTT+TGG 0.242380 8.4:+40850075 None:intergenic
CATTGTTGCGACGGAAGTTC+TGG 0.309789 8.4:+40849969 None:intergenic
ATGATGTAAATCGCGAAATT+CGG 0.311734 8.4:+40848944 None:intergenic
GACCCAGCTGTATCTTCTTA+AGG 0.322964 8.4:-40848445 None:intergenic
TGATGTAAATCGCGAAATTC+GGG 0.330334 8.4:+40848945 None:intergenic
CCTAGGGCAGCCAGAGATAA+AGG 0.341297 8.4:+40849401 None:intergenic
TGTTGGTAACTTTCACAGTA+TGG 0.341967 8.4:-40849241 MS.gene63177:CDS
CTCTTAATCGTTTGAGAGAA+TGG 0.349597 8.4:+40848506 None:intergenic
ATGTTTCAACTCAAATCTTC+TGG 0.366123 8.4:-40848904 MS.gene63177:CDS
TTCTAATACTGCAGTCTTCA+CGG 0.375556 8.4:-40849534 MS.gene63177:intron
CTCGGATGAGGTTGAGAATA+GGG 0.388430 8.4:+40849169 None:intergenic
GAATTAGTTTCCTTTATCTC+TGG 0.402305 8.4:-40849411 MS.gene63177:CDS
TCAAGGACTTGAAGATGAAT+TGG 0.403742 8.4:-40848617 MS.gene63177:CDS
CCTTTATCTCTGGCTGCCCT+AGG 0.405572 8.4:-40849401 MS.gene63177:CDS
CTGACCTATGTTCACATAGA+AGG 0.414878 8.4:-40848643 MS.gene63177:CDS
GTCTCTCGGCGAAGATATTG+TGG 0.415832 8.4:-40849442 MS.gene63177:CDS
AATTCGGGATAATTTAGAAC+AGG 0.421287 8.4:+40848960 None:intergenic
TATCTCTGGCTGCCCTAGGC+TGG 0.427111 8.4:-40849397 MS.gene63177:CDS
GAAGAAATCGACGGCGGAGG+AGG 0.431796 8.4:-40850063 MS.gene63177:CDS
GAAAGTCGAAGTCGAAGATC+TGG 0.433372 8.4:+40849905 None:intergenic
CTCCTAGATATCTTCATCTC+TGG 0.433430 8.4:-40849936 MS.gene63177:CDS
TTGCGACGGAAGTTCTGGTT+GGG 0.435631 8.4:+40849974 None:intergenic
ACCATTGAGAACATGGCAAC+TGG 0.439386 8.4:-40848701 MS.gene63177:CDS
TTCGAATGTCGCGGGATTTG+CGG 0.439604 8.4:+40849827 None:intergenic
TTGGGAGGAAGGACATGATA+TGG 0.449100 8.4:+40849992 None:intergenic
ACTTACTTTAATATAAATGA+AGG 0.456907 8.4:-40849282 MS.gene63177:CDS
TTCCAGAGATGAAGATATCT+AGG 0.456977 8.4:+40849934 None:intergenic
CCTCCAACGATGATTTCACT+TGG 0.463758 8.4:-40849305 MS.gene63177:CDS
TGCCATGTTCTCAATGGTAG+TGG 0.473129 8.4:+40848706 None:intergenic
CTTCAGTGATGGAAAATAAA+CGG 0.475416 8.4:+40849470 None:intergenic
GGAAGGTTTCACGAGAACTT+TGG 0.476254 8.4:+40849326 None:intergenic
CACTTCTGTCATCTCGGATG+AGG 0.484315 8.4:+40849157 None:intergenic
AAGATAGAGTTGTTGAAGGT+GGG 0.486842 8.4:+40849717 None:intergenic
ACATGCAAAGTTTCCAGCCT+AGG 0.487752 8.4:+40849384 None:intergenic
TCACCTTAAGAAGATACAGC+TGG 0.488366 8.4:+40848442 None:intergenic
ATAGATCCTCGTCATCATCT+AGG 0.488635 8.4:+40849118 None:intergenic
GAAAATAAACGGAGTTTGTA+CGG 0.493319 8.4:+40849481 None:intergenic
TCTCGGATGAGGTTGAGAAT+AGG 0.495111 8.4:+40849168 None:intergenic
GCACTAACCTCGTTTCCCTC+AGG 0.498581 8.4:-40849645 MS.gene63177:intron
CAGCTGGGTCAAATTTAAGT+TGG 0.508915 8.4:+40848458 None:intergenic
GAAGAAGAAATCGACGGCGG+AGG 0.509809 8.4:-40850066 MS.gene63177:CDS
ACGTGGATTCTTGCTGCCAT+TGG 0.513966 8.4:-40849742 MS.gene63177:CDS
TTGTTGAAGGTGGGGTCCAA+TGG 0.515637 8.4:+40849726 None:intergenic
TCCAGTTGCCATGTTCTCAA+TGG 0.519376 8.4:+40848700 None:intergenic
GGAAGTTCTGGTTGGGAGGA+AGG 0.519935 8.4:+40849981 None:intergenic
GTTCACATAGAAGGATATCA+AGG 0.520078 8.4:-40848634 MS.gene63177:CDS
CTTCTATGTGAACATAGGTC+AGG 0.522459 8.4:+40848644 None:intergenic
GAGGCGATGACCTCACCGAC+AGG 0.523086 8.4:-40850044 MS.gene63177:CDS
GAAATTCACTTCTGTCATCT+CGG 0.527470 8.4:+40849151 None:intergenic
TACAAAGATAGAGTTGTTGA+AGG 0.530392 8.4:+40849713 None:intergenic
GTTGCGACGGAAGTTCTGGT+TGG 0.533913 8.4:+40849973 None:intergenic
AAAGATAGAGTTGTTGAAGG+TGG 0.535343 8.4:+40849716 None:intergenic
ATAAAGGAAACTAATTCAGT+TGG 0.537900 8.4:+40849417 None:intergenic
CATGCAAAGTTTCCAGCCTA+GGG 0.543778 8.4:+40849385 None:intergenic
ATATCCTTCTATGTGAACAT+AGG 0.544669 8.4:+40848639 None:intergenic
CCAAGTGAAATCATCGTTGG+AGG 0.545248 8.4:+40849305 None:intergenic
CACCTTAAGAAGATACAGCT+GGG 0.545919 8.4:+40848443 None:intergenic
CGCTGAATCCACGATGGCGG+AGG 0.546316 8.4:-40850106 None:intergenic
TTTAGAACAGGGGCATGAGT+TGG 0.553956 8.4:+40848972 None:intergenic
GTGAAATCATCGTTGGAGGA+AGG 0.558550 8.4:+40849309 None:intergenic
ATATGGCATAGTACAGAGTC+CGG 0.561225 8.4:+40850009 None:intergenic
AACAAATTCAGTTTCAGCCA+CGG 0.563124 8.4:+40849194 None:intergenic
AACCACTACCATTGAGAACA+TGG 0.563418 8.4:-40848708 MS.gene63177:CDS
TCTCTCGGCGAAGATATTGT+GGG 0.566500 8.4:-40849441 MS.gene63177:CDS
AGAGACAACGTCTTCAGTGA+TGG 0.567151 8.4:+40849459 None:intergenic
CGGTAGAGTGCTCAGCCTGT+CGG 0.570238 8.4:+40850029 None:intergenic
CGACAGGCTGAGCACTCTAC+CGG 0.570912 8.4:-40850028 MS.gene63177:CDS
TATGTGAACATAGGTCAGGT+TGG 0.571585 8.4:+40848648 None:intergenic
GCAAATCCCGCGACATTCGA+AGG 0.578368 8.4:-40849825 MS.gene63177:CDS
GACAGAAGTGAATTTCATAA+CGG 0.579647 8.4:-40849144 MS.gene63177:CDS
AGATAGAGTTGTTGAAGGTG+GGG 0.580102 8.4:+40849718 None:intergenic
TCACTGAAGACGTTGTCTCT+CGG 0.580179 8.4:-40849456 MS.gene63177:CDS
TCTTCACGGTTCAATCGAAA+CGG 0.582481 8.4:-40849520 MS.gene63177:CDS
AGTCCAAGTGAAATCATCGT+TGG 0.583850 8.4:+40849302 None:intergenic
ACGGAACCTTCGAATGTCGC+GGG 0.584834 8.4:+40849819 None:intergenic
AGAAGATTTGAGTTGAAACA+TGG 0.586977 8.4:+40848906 None:intergenic
GACGAGACTCATTGTTGCGA+CGG 0.590038 8.4:+40849960 None:intergenic
CGACGGAAGTTCTGGTTGGG+AGG 0.592790 8.4:+40849977 None:intergenic
GATTTGAGTTGAAACATGGA+AGG 0.600986 8.4:+40848910 None:intergenic
TGGCACATGTCAGAGCCCCT+TGG 0.606191 8.4:+40848478 None:intergenic
CTGAGTTGTAACTCATCGCG+TGG 0.606212 8.4:+40849775 None:intergenic
GAGTGCTCAGCCTGTCGGTG+AGG 0.620743 8.4:+40850034 None:intergenic
TAGATATCTTCATCTCTGGA+AGG 0.622526 8.4:-40849932 MS.gene63177:CDS
GTTGCGTCATTCGACATCAA+AGG 0.623983 8.4:-40849088 MS.gene63177:intron
CAATTGATGAAGAGTGAACG+AGG 0.626927 8.4:+40849667 None:intergenic
TTCGGGATAATTTAGAACAG+GGG 0.629854 8.4:+40848962 None:intergenic
GACGGAACCTTCGAATGTCG+CGG 0.632919 8.4:+40849818 None:intergenic
CGGCCTCCTAGATGATGACG+AGG 0.633593 8.4:-40849124 MS.gene63177:CDS
TGGTAACTTTCACAGTATGG+TGG 0.637517 8.4:-40849238 MS.gene63177:CDS
TCTCATAATTCAGAGCAACA+AGG 0.643040 8.4:-40848847 MS.gene63177:intron
ATTCGGGATAATTTAGAACA+GGG 0.649291 8.4:+40848961 None:intergenic
AAAGCAGAAGAAGAAATCGA+CGG 0.649356 8.4:-40850072 MS.gene63177:CDS
CATCAAATGTGAGAGTGAGA+CGG 0.650223 8.4:+40849800 None:intergenic
AACGATTAAGAGACATACCA+AGG 0.658244 8.4:-40848495 MS.gene63177:CDS
AACTCAGGAATGCTGAAACG+TGG 0.660563 8.4:-40849759 MS.gene63177:CDS
GATCCTCGTCATCATCTAGG+AGG 0.666579 8.4:+40849121 None:intergenic
ACGATTAAGAGACATACCAA+GGG 0.671430 8.4:-40848494 MS.gene63177:CDS
TTCAATGAACAAAACAGACA+AGG 0.679515 8.4:-40848530 MS.gene63177:CDS
AAAGTTACCTGAGGGAAACG+AGG 0.681818 8.4:+40849638 None:intergenic
CACGCGATGAGTTACAACTC+AGG 0.685874 8.4:-40849774 MS.gene63177:CDS
CGATTAAGAGACATACCAAG+GGG 0.728593 8.4:-40848493 MS.gene63177:CDS
GCAGAAGAAGAAATCGACGG+CGG 0.735395 8.4:-40850069 MS.gene63177:CDS
AATTGATGAAGAGTGAACGA+GGG 0.761342 8.4:+40849668 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTACTTTAATATAAATGA+AGG - chr8.4:40849258-40849277 MS.gene63177:CDS 15.0%
!!! AAATGAAGGATGTTTTAAAT+TGG - chr8.4:40849272-40849291 MS.gene63177:CDS 20.0%
!!! AATGAAGGATGTTTTAAATT+GGG - chr8.4:40849273-40849292 MS.gene63177:CDS 20.0%
!!! ACAAATATATTTTTGAGTCA+CGG - chr8.4:40849781-40849800 MS.gene63177:CDS 20.0%
!!! ATATTTTTACATGTGTAGTT+TGG - chr8.4:40849499-40849518 MS.gene63177:CDS 20.0%
! ATAAAGGAAACTAATTCAGT+TGG + chr8.4:40849126-40849145 None:intergenic 25.0%
!! CATTCGTTGAATATATTTTG+AGG - chr8.4:40849946-40849965 MS.gene63177:CDS 25.0%
!!! GTTGAATATATTTTGAGGAA+CGG - chr8.4:40849951-40849970 MS.gene63177:CDS 25.0%
ATATCCTTCTATGTGAACAT+AGG + chr8.4:40849904-40849923 None:intergenic 30.0%
ATGATGTAAATCGCGAAATT+CGG + chr8.4:40849599-40849618 None:intergenic 30.0%
ATGTTTCAACTCAAATCTTC+TGG - chr8.4:40849636-40849655 MS.gene63177:intron 30.0%
GAAAATAAACGGAGTTTGTA+CGG + chr8.4:40849062-40849081 None:intergenic 30.0%
GACAGAAGTGAATTTCATAA+CGG - chr8.4:40849396-40849415 MS.gene63177:CDS 30.0%
TACAAAGATAGAGTTGTTGA+AGG + chr8.4:40848830-40848849 None:intergenic 30.0%
TTCAATGAACAAAACAGACA+AGG - chr8.4:40850010-40850029 MS.gene63177:CDS 30.0%
! AATTCGGGATAATTTAGAAC+AGG + chr8.4:40849583-40849602 None:intergenic 30.0%
! ATTCGGGATAATTTAGAACA+GGG + chr8.4:40849582-40849601 None:intergenic 30.0%
! CTTCAGTGATGGAAAATAAA+CGG + chr8.4:40849073-40849092 None:intergenic 30.0%
! GAATTAGTTTCCTTTATCTC+TGG - chr8.4:40849129-40849148 MS.gene63177:CDS 30.0%
!! AGAAGATTTGAGTTGAAACA+TGG + chr8.4:40849637-40849656 None:intergenic 30.0%
!! TGTTTTAAATTGGGCATTGT+TGG - chr8.4:40849282-40849301 MS.gene63177:CDS 30.0%
!!! TATATTTTTGAGTCACGGTA+TGG - chr8.4:40849786-40849805 MS.gene63177:CDS 30.0%
AAAGATAGAGTTGTTGAAGG+TGG + chr8.4:40848827-40848846 None:intergenic 35.0%
AAAGCAGAAGAAGAAATCGA+CGG - chr8.4:40848468-40848487 MS.gene63177:CDS 35.0%
AACAAATTCAGTTTCAGCCA+CGG + chr8.4:40849349-40849368 None:intergenic 35.0%
AACGATTAAGAGACATACCA+AGG - chr8.4:40850045-40850064 MS.gene63177:CDS 35.0%
AAGATAGAGTTGTTGAAGGT+GGG + chr8.4:40848826-40848845 None:intergenic 35.0%
AATTGATGAAGAGTGAACGA+GGG + chr8.4:40848875-40848894 None:intergenic 35.0%
ACGATTAAGAGACATACCAA+GGG - chr8.4:40850046-40850065 MS.gene63177:CDS 35.0%
CTCTTAATCGTTTGAGAGAA+TGG + chr8.4:40850037-40850056 None:intergenic 35.0%
GAAATTCACTTCTGTCATCT+CGG + chr8.4:40849392-40849411 None:intergenic 35.0%
GTTCACATAGAAGGATATCA+AGG - chr8.4:40849906-40849925 MS.gene63177:CDS 35.0%
TACACGATAACAACAAGTGA+TGG + chr8.4:40849538-40849557 None:intergenic 35.0%
TAGATATCTTCATCTCTGGA+AGG - chr8.4:40848608-40848627 MS.gene63177:CDS 35.0%
TATCTGCAAAAGTTACCTGA+GGG + chr8.4:40848913-40848932 None:intergenic 35.0%
TCAAGGACTTGAAGATGAAT+TGG - chr8.4:40849923-40849942 MS.gene63177:CDS 35.0%
TCTCATAATTCAGAGCAACA+AGG - chr8.4:40849693-40849712 MS.gene63177:CDS 35.0%
TGATGTAAATCGCGAAATTC+GGG + chr8.4:40849598-40849617 None:intergenic 35.0%
TTCCAGAGATGAAGATATCT+AGG + chr8.4:40848609-40848628 None:intergenic 35.0%
TTCTAATACTGCAGTCTTCA+CGG - chr8.4:40849006-40849025 MS.gene63177:intron 35.0%
! TCGATTTCTTCTTCTGCTTT+TGG + chr8.4:40848468-40848487 None:intergenic 35.0%
! TTCGGGATAATTTAGAACAG+GGG + chr8.4:40849581-40849600 None:intergenic 35.0%
!! ATTTCTTCTTCTGCTTTTGG+TGG + chr8.4:40848465-40848484 None:intergenic 35.0%
!! GATTTGAGTTGAAACATGGA+AGG + chr8.4:40849633-40849652 None:intergenic 35.0%
!! TGTTGGTAACTTTCACAGTA+TGG - chr8.4:40849299-40849318 MS.gene63177:CDS 35.0%
AACCACTACCATTGAGAACA+TGG - chr8.4:40849832-40849851 MS.gene63177:CDS 40.0%
AGATAGAGTTGTTGAAGGTG+GGG + chr8.4:40848825-40848844 None:intergenic 40.0%
AGTCCAAGTGAAATCATCGT+TGG + chr8.4:40849241-40849260 None:intergenic 40.0%
ATAGATCCTCGTCATCATCT+AGG + chr8.4:40849425-40849444 None:intergenic 40.0%
ATATGGCATAGTACAGAGTC+CGG + chr8.4:40848534-40848553 None:intergenic 40.0%
CAATTGATGAAGAGTGAACG+AGG + chr8.4:40848876-40848895 None:intergenic 40.0%
CAGCTGGGTCAAATTTAAGT+TGG + chr8.4:40850085-40850104 None:intergenic 40.0%
CATCAAATGTGAGAGTGAGA+CGG + chr8.4:40848743-40848762 None:intergenic 40.0%
CCACTCAACTTCGTTCAAAA+AGG - chr8.4:40848659-40848678 MS.gene63177:CDS 40.0%
CGATTAAGAGACATACCAAG+GGG - chr8.4:40850047-40850066 MS.gene63177:CDS 40.0%
CTCCTAGATATCTTCATCTC+TGG - chr8.4:40848604-40848623 MS.gene63177:CDS 40.0%
CTGACCTATGTTCACATAGA+AGG - chr8.4:40849897-40849916 MS.gene63177:CDS 40.0%
CTTCTATGTGAACATAGGTC+AGG + chr8.4:40849899-40849918 None:intergenic 40.0%
GTATCTGCAAAAGTTACCTG+AGG + chr8.4:40848914-40848933 None:intergenic 40.0%
TATGTGAACATAGGTCAGGT+TGG + chr8.4:40849895-40849914 None:intergenic 40.0%
TCTTCACGGTTCAATCGAAA+CGG - chr8.4:40849020-40849039 MS.gene63177:intron 40.0%
TGGTAACTTTCACAGTATGG+TGG - chr8.4:40849302-40849321 MS.gene63177:CDS 40.0%
! CCTTTTTGAACGAAGTTGAG+TGG + chr8.4:40848662-40848681 None:intergenic 40.0%
! GTTTCTTGATGTCTTTTCCG+TGG - chr8.4:40849329-40849348 MS.gene63177:CDS 40.0%
AAAGTTACCTGAGGGAAACG+AGG + chr8.4:40848905-40848924 None:intergenic 45.0%
AACTCAGGAATGCTGAAACG+TGG - chr8.4:40848781-40848800 MS.gene63177:intron 45.0%
ACATGCAAAGTTTCCAGCCT+AGG + chr8.4:40849159-40849178 None:intergenic 45.0%
ACCATTGAGAACATGGCAAC+TGG - chr8.4:40849839-40849858 MS.gene63177:CDS 45.0%
AGAGACAACGTCTTCAGTGA+TGG + chr8.4:40849084-40849103 None:intergenic 45.0%
CATGCAAAGTTTCCAGCCTA+GGG + chr8.4:40849158-40849177 None:intergenic 45.0%
CCAAGTGAAATCATCGTTGG+AGG + chr8.4:40849238-40849257 None:intergenic 45.0%
CCTCCAACGATGATTTCACT+TGG - chr8.4:40849235-40849254 MS.gene63177:CDS 45.0%
CTCGGATGAGGTTGAGAATA+GGG + chr8.4:40849374-40849393 None:intergenic 45.0%
GAAAGTCGAAGTCGAAGATC+TGG + chr8.4:40848638-40848657 None:intergenic 45.0%
GGAAGGTTTCACGAGAACTT+TGG + chr8.4:40849217-40849236 None:intergenic 45.0%
GTGAAATCATCGTTGGAGGA+AGG + chr8.4:40849234-40849253 None:intergenic 45.0%
GTTCCTCCTACAACCAACAA+GGG + chr8.4:40849815-40849834 None:intergenic 45.0%
GTTGCGTCATTCGACATCAA+AGG - chr8.4:40849452-40849471 MS.gene63177:CDS 45.0%
TCACTGAAGACGTTGTCTCT+CGG - chr8.4:40849084-40849103 MS.gene63177:intron 45.0%
TCCAGTTGCCATGTTCTCAA+TGG + chr8.4:40849843-40849862 None:intergenic 45.0%
TCTCGGATGAGGTTGAGAAT+AGG + chr8.4:40849375-40849394 None:intergenic 45.0%
TCTCTCGGCGAAGATATTGT+GGG - chr8.4:40849099-40849118 MS.gene63177:CDS 45.0%
TGCCATGTTCTCAATGGTAG+TGG + chr8.4:40849837-40849856 None:intergenic 45.0%
TTGGGAGGAAGGACATGATA+TGG + chr8.4:40848551-40848570 None:intergenic 45.0%
TTGTTATCGTGTACGTGCAG+TGG - chr8.4:40849545-40849564 MS.gene63177:intron 45.0%
! TTTAGAACAGGGGCATGAGT+TGG + chr8.4:40849571-40849590 None:intergenic 45.0%
ACGTGGATTCTTGCTGCCAT+TGG - chr8.4:40848798-40848817 MS.gene63177:intron 50.0%
CACGCGATGAGTTACAACTC+AGG - chr8.4:40848766-40848785 MS.gene63177:intron 50.0%
CACTTCTGTCATCTCGGATG+AGG + chr8.4:40849386-40849405 None:intergenic 50.0%
CATTGTTGCGACGGAAGTTC+TGG + chr8.4:40848574-40848593 None:intergenic 50.0%
CTGAGTTGTAACTCATCGCG+TGG + chr8.4:40848768-40848787 None:intergenic 50.0%
GACGAGACTCATTGTTGCGA+CGG + chr8.4:40848583-40848602 None:intergenic 50.0%
GATCCTCGTCATCATCTAGG+AGG + chr8.4:40849422-40849441 None:intergenic 50.0%
GCAGAAGAAGAAATCGACGG+CGG - chr8.4:40848471-40848490 MS.gene63177:CDS 50.0%
GGTTCCTCCTACAACCAACA+AGG + chr8.4:40849816-40849835 None:intergenic 50.0%
GTCTCTCGGCGAAGATATTG+TGG - chr8.4:40849098-40849117 MS.gene63177:CDS 50.0%
TTCGAATGTCGCGGGATTTG+CGG + chr8.4:40848716-40848735 None:intergenic 50.0%
TTGTTGAAGGTGGGGTCCAA+TGG + chr8.4:40848817-40848836 None:intergenic 50.0%
! TGGTGTCCCTTGTTGGTTGT+AGG - chr8.4:40849806-40849825 MS.gene63177:CDS 50.0%
! TGTCCCTTGTTGGTTGTAGG+AGG - chr8.4:40849809-40849828 MS.gene63177:CDS 50.0%
! TTGCGACGGAAGTTCTGGTT+GGG + chr8.4:40848569-40848588 None:intergenic 50.0%
ACGGAACCTTCGAATGTCGC+GGG + chr8.4:40848724-40848743 None:intergenic 55.0%
CACGGTATGGTGTCCCTTGT+TGG - chr8.4:40849799-40849818 MS.gene63177:CDS 55.0%
CCTAGGGCAGCCAGAGATAA+AGG + chr8.4:40849142-40849161 None:intergenic 55.0%
CCTTTATCTCTGGCTGCCCT+AGG - chr8.4:40849139-40849158 MS.gene63177:CDS 55.0%
GAAGAAGAAATCGACGGCGG+AGG - chr8.4:40848474-40848493 MS.gene63177:CDS 55.0%
GACGGAACCTTCGAATGTCG+CGG + chr8.4:40848725-40848744 None:intergenic 55.0%
GCAAATCCCGCGACATTCGA+AGG - chr8.4:40848715-40848734 MS.gene63177:CDS 55.0%
GCACTAACCTCGTTTCCCTC+AGG - chr8.4:40848895-40848914 MS.gene63177:CDS 55.0%
! GGAAGTTCTGGTTGGGAGGA+AGG + chr8.4:40848562-40848581 None:intergenic 55.0%
! GTTGCGACGGAAGTTCTGGT+TGG + chr8.4:40848570-40848589 None:intergenic 55.0%
CGACAGGCTGAGCACTCTAC+CGG - chr8.4:40848512-40848531 MS.gene63177:CDS 60.0%
GAAGAAATCGACGGCGGAGG+AGG - chr8.4:40848477-40848496 MS.gene63177:CDS 60.0%
TATCTCTGGCTGCCCTAGGC+TGG - chr8.4:40849143-40849162 MS.gene63177:CDS 60.0%
TGGCACATGTCAGAGCCCCT+TGG + chr8.4:40850065-40850084 None:intergenic 60.0%
! CGACGGAAGTTCTGGTTGGG+AGG + chr8.4:40848566-40848585 None:intergenic 60.0%
! CGGCCTCCTAGATGATGACG+AGG - chr8.4:40849416-40849435 MS.gene63177:CDS 60.0%
! CGGTAGAGTGCTCAGCCTGT+CGG + chr8.4:40848514-40848533 None:intergenic 60.0%
GAGGCGATGACCTCACCGAC+AGG - chr8.4:40848496-40848515 MS.gene63177:CDS 65.0%
GAGTGCTCAGCCTGTCGGTG+AGG + chr8.4:40848509-40848528 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 40848447 40850115 40848447 ID=MS.gene63177
chr8.4 mRNA 40848447 40850115 40848447 ID=MS.gene63177.t1;Parent=MS.gene63177
chr8.4 exon 40849646 40850115 40849646 ID=MS.gene63177.t1.exon1;Parent=MS.gene63177.t1
chr8.4 CDS 40849646 40850115 40849646 ID=cds.MS.gene63177.t1;Parent=MS.gene63177.t1
chr8.4 exon 40849089 40849542 40849089 ID=MS.gene63177.t1.exon2;Parent=MS.gene63177.t1
chr8.4 CDS 40849089 40849542 40849089 ID=cds.MS.gene63177.t1;Parent=MS.gene63177.t1
chr8.4 exon 40848848 40848997 40848848 ID=MS.gene63177.t1.exon3;Parent=MS.gene63177.t1
chr8.4 CDS 40848848 40848997 40848848 ID=cds.MS.gene63177.t1;Parent=MS.gene63177.t1
chr8.4 exon 40848447 40848734 40848447 ID=MS.gene63177.t1.exon4;Parent=MS.gene63177.t1
chr8.4 CDS 40848447 40848734 40848447 ID=cds.MS.gene63177.t1;Parent=MS.gene63177.t1
Gene Sequence

>MS.gene63177

ATGGCGGAGGAGTCACCACCAAAAGCAGAAGAAGAAATCGACGGCGGAGGAGGCGATGACCTCACCGACAGGCTGAGCACTCTACCGGACTCTGTACTATGCCATATCATGTCCTTCCTCCCAACCAGAACTTCCGTCGCAACAATGAGTCTCGTCTCTCCTAGATATCTTCATCTCTGGAAGGATCTCCAGATCTTCGACTTCGACTTTCACCACTCAACTTCGTTCAAAAAGGTTGCATTATTCATTAACTCTGTTCTCACTCTCCGCAAATCCCGCGACATTCGAAGGTTCCGTCTCACTCTCACATTTGATGATCCACGCGATGAGTTACAACTCAGGAATGCTGAAACGTGGATTCTTGCTGCCATTGGACCCCACCTTCAACAACTCTATCTTTGTATTGACGAGTCTTGTATTGACATCCCTCGTTCACTCTTCATCAATTGCACTAACCTCGTTTCCCTCAGGTAACTTTTGCAGATACTCATGTTTTAACGAAAACTTATGATGATTTGTTTTTATGTTCAAACTCACTTTCACTTTCGAATCATATTTTTTCTAATACTGCAGTCTTCACGGTTCAATCGAAACGGCACACTGCAATCTTGACCGTACAAACTCCGTTTATTTTCCATCACTGAAGACGTTGTCTCTCGGCGAAGATATTGTGGGTCCAACTGAATTAGTTTCCTTTATCTCTGGCTGCCCTAGGCTGGAAACTTTGCATGTTTACTTTTACATTGTCTCTCAAGAAATGTCTTTCACCAAAGTTCTCGTGAAACCTTCCTCCAACGATGATTTCACTTGGACTTACTTTAATATAAATGAAGGATGTTTTAAATTGGGCATTGTTGGTAACTTTCACAGTATGGTGGAAGCGTTTCTTGATGTCTTTTCCGTGGCTGAAACTGAATTTGTTGACCCTATTCTCAACCTCATCCGAGATGACAGAAGTGAATTTCATAACGGCCTCCTAGATGATGACGAGGATCTATATCTAGAGTTGCGTCATTCGACATCAAAGGTGAAGTTTTACTTTTATGCTTTATATATTTTTACATGTGTAGTTTGGTTCTAATATTGCGCCATCACTTGTTGTTATCGTGTACGTGCAGTGGCCAACTCATGCCCCTGTTCTAAATTATCCCGAATTTCGCGATTTACATCATCTGAAGTTCATCCTTCCATGTTTCAACTCAAATCTTCTGGTCAATGTTCTTGAGCAATGTCACATGCTTCAAGTTCTCATAATTCAGAGCAACAAGGTTTGTTGAATTTTCAAATTTAATTCAAACATTCTCAGAGCAATGCATGCATGTCTCTATCTGTACACAAATATATTTTTGAGTCACGGTATGGTGTCCCTTGTTGGTTGTAGGAGGAACCACTACCATTGAGAACATGGCAACTGGAGTCAACAACAGTTTCTGAATGTCTCAAATCCAACCTGACCTATGTTCACATAGAAGGATATCAAGGACTTGAAGATGAATTGGCATTCGTTGAATATATTTTGAGGAACGGAGTTGTTTTGAAGACAATACTTATTTTTGTTGATATTTCAATGAACAAAACAGACAAGGACCATTCTCTCAAACGATTAAGAGACATACCAAGGGGCTCTGACATGTGCCAACTTAAATTTGACCCAGCTGTATCTTCTTAA
Protein sequence

>MS.gene63177.t1

MAEESPPKAEEEIDGGGGDDLTDRLSTLPDSVLCHIMSFLPTRTSVATMSLVSPRYLHLWKDLQIFDFDFHHSTSFKKVALFINSVLTLRKSRDIRRFRLTLTFDDPRDELQLRNAETWILAAIGPHLQQLYLCIDESCIDIPRSLFINCTNLVSLSLHGSIETAHCNLDRTNSVYFPSLKTLSLGEDIVGPTELVSFISGCPRLETLHVYFYIVSQEMSFTKVLVKPSSNDDFTWTYFNINEGCFKLGIVGNFHSMVEAFLDVFSVAETEFVDPILNLIRDDRSEFHNGLLDDDEDLYLELRHSTSKWPTHAPVLNYPEFRDLHHLKFILPCFNSNLLVNVLEQCHMLQVLIIQSNKEEPLPLRTWQLESTTVSECLKSNLTYVHIEGYQGLEDELAFVEYILRNGVVLKTILIFVDISMNKTDKDHSLKRLRDIPRGSDMCQLKFDPAVSS