AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25415

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25415.t1 MTR_8g468810 92.771 415 28 1 1 415 51 463 0.0 779
MS.gene25415.t1 MTR_8g468810 93.808 323 18 1 1 323 51 371 0.0 607
MS.gene25415.t1 MTR_8g058970 65.207 434 131 3 1 415 45 477 0.0 529
MS.gene25415.t1 MTR_8g468810 93.657 268 15 1 1 268 51 316 5.55e-178 499
MS.gene25415.t1 MTR_8g468810 94.007 267 14 1 1 267 51 315 1.06e-177 498
MS.gene25415.t1 MTR_8g468810 94.007 267 14 1 1 267 51 315 2.16e-177 499
MS.gene25415.t1 MTR_2g083840 55.609 419 174 8 1 415 47 457 6.42e-143 415
MS.gene25415.t1 MTR_8g468840 50.000 422 188 10 2 413 40 448 3.33e-121 359
MS.gene25415.t1 MTR_8g062270 48.485 429 190 11 1 414 41 453 6.16e-121 359
MS.gene25415.t1 MTR_4g032730 48.190 442 181 10 3 414 43 466 4.55e-119 355
MS.gene25415.t1 MTR_8g467800 49.883 427 182 10 2 415 46 453 6.24e-119 354
MS.gene25415.t1 MTR_5g092960 49.533 428 187 11 3 414 47 461 2.42e-114 342
MS.gene25415.t1 MTR_8g468760 49.415 427 161 10 1 414 34 418 5.96e-113 337
MS.gene25415.t1 MTR_8g014260 42.891 422 217 15 4 415 44 451 5.04e-91 282
MS.gene25415.t1 MTR_8g058965 41.995 431 219 12 2 415 40 456 6.90e-84 264
MS.gene25415.t1 MTR_5g092960 49.249 333 149 9 3 323 47 371 7.41e-83 259
MS.gene25415.t1 MTR_8g469020 38.799 433 231 13 2 415 34 451 4.21e-81 257
MS.gene25415.t1 MTR_8g445780 43.869 367 183 11 4 365 41 389 4.88e-78 247
MS.gene25415.t1 MTR_8g467800 54.753 263 107 5 2 259 46 301 6.49e-74 234
MS.gene25415.t1 MTR_8g070950 38.161 435 208 15 1 415 41 434 2.26e-73 236
MS.gene25415.t1 MTR_4g050250 41.406 384 186 12 1 357 47 418 3.04e-68 223
MS.gene25415.t1 MTR_8g062080 37.559 426 226 15 1 415 49 445 2.18e-63 211
MS.gene25415.t1 MTR_8g062010 37.441 422 231 14 1 415 35 430 2.77e-63 210
MS.gene25415.t1 MTR_8g077460 34.928 418 186 11 4 415 38 375 7.15e-62 204
MS.gene25415.t1 MTR_8g062310 36.715 414 221 13 4 415 54 428 1.30e-61 205
MS.gene25415.t1 MTR_8g062300 35.491 448 234 14 1 415 52 477 3.50e-57 195
MS.gene25415.t1 MTR_4g024710 42.434 304 156 11 117 415 58 347 6.93e-55 185
MS.gene25415.t1 MTR_8g014160 38.628 277 158 7 2 269 67 340 1.44e-47 168
MS.gene25415.t1 MTR_8g014160 48.718 78 37 1 338 415 351 425 6.13e-14 73.6
MS.gene25415.t1 MTR_8g014210 45.679 243 119 9 174 415 1 231 5.65e-47 161
MS.gene25415.t1 MTR_4g024745 40.925 281 152 9 115 391 10 280 2.29e-43 153
MS.gene25415.t1 MTR_8g467790 33.243 370 180 14 1 355 174 491 4.33e-40 150
MS.gene25415.t1 MTR_5g021550 48.276 116 57 3 4 116 47 162 8.90e-27 105
MS.gene25415.t1 MTR_8g014290 44.715 123 59 5 4 117 38 160 4.01e-24 98.2
MS.gene25415.t1 MTR_2g086020 43.919 148 66 6 1 142 1 137 9.47e-22 95.5
MS.gene25415.t1 MTR_2g086020 42.202 109 61 1 307 415 209 315 3.66e-18 85.1
MS.gene25415.t1 MTR_5g080710 28.401 419 256 18 2 407 59 446 6.23e-20 92.0
MS.gene25415.t1 MTR_4g113810 33.529 170 106 5 57 221 1 168 4.04e-18 81.6
MS.gene25415.t1 MTR_5g084070 27.110 391 238 16 2 380 39 394 2.01e-17 84.3
MS.gene25415.t1 MTR_7g098510 25.532 423 231 12 2 415 44 391 4.56e-16 79.7
MS.gene25415.t1 MTR_4g024720 37.313 134 61 6 4 116 45 176 6.01e-16 76.6
MS.gene25415.t1 MTR_4g056040 28.612 353 213 14 41 372 99 433 1.58e-15 78.6
MS.gene25415.t1 MTR_1g041245 35.465 172 88 6 2 166 37 192 2.70e-15 77.4
MS.gene25415.t1 MTR_8g014220 40.323 124 62 5 43 161 44 160 2.80e-15 73.6
MS.gene25415.t1 MTR_4g056070 29.326 341 190 15 41 354 109 425 4.61e-15 77.0
MS.gene25415.t1 MTR_7g098570 24.279 416 237 12 2 411 46 389 1.53e-12 69.3
MS.gene25415.t1 MTR_7g033915 26.279 430 198 18 2 414 37 364 1.69e-12 68.9
MS.gene25415.t1 MTR_8g014230 50.685 73 32 2 333 405 27 95 1.74e-12 63.9
MS.gene25415.t1 MTR_4g056020 27.128 376 228 16 18 372 53 403 3.01e-12 68.6
MS.gene25415.t1 MTR_7g032380 32.394 142 86 3 281 415 218 356 2.69e-11 65.1
MS.gene25415.t1 MTR_3g082890 26.415 424 240 17 2 415 123 484 4.44e-11 64.7
MS.gene25415.t1 MTR_3g036250 27.753 227 136 6 199 412 152 363 5.39e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25415.t1 AT5G56420 26.790 433 256 18 2 415 32 422 7.07e-24 103
MS.gene25415.t1 AT5G56420 26.790 433 256 18 2 415 32 422 7.07e-24 103
MS.gene25415.t1 AT5G56420 26.790 433 256 18 2 415 32 422 7.07e-24 103
MS.gene25415.t1 AT4G26340 27.273 429 260 17 2 415 28 419 3.56e-23 101
MS.gene25415.t1 AT1G16930 27.009 448 254 19 2 415 41 449 7.33e-23 100
MS.gene25415.t1 AT1G16930 27.009 448 254 19 2 415 41 449 7.33e-23 100
MS.gene25415.t1 AT1G16930 27.009 448 254 19 2 415 41 449 7.33e-23 100
MS.gene25415.t1 AT5G56370 26.559 433 258 16 2 414 28 420 7.50e-21 94.4
MS.gene25415.t1 AT5G56370 26.559 433 258 16 2 414 28 420 7.50e-21 94.4
MS.gene25415.t1 AT5G62970 28.165 387 240 14 41 414 87 448 5.08e-20 92.0
MS.gene25415.t1 AT5G56700 28.493 365 221 15 41 392 22 359 1.44e-19 89.7
MS.gene25415.t1 AT5G60610 27.481 393 239 15 2 381 28 387 7.34e-19 88.2
MS.gene25415.t1 AT5G60610 27.481 393 239 15 2 381 28 387 7.34e-19 88.2
MS.gene25415.t1 AT1G32375 24.651 430 271 15 3 415 29 422 3.03e-18 86.7
MS.gene25415.t1 AT1G32375 24.651 430 271 15 3 415 29 422 3.03e-18 86.7
MS.gene25415.t1 AT4G10420 26.012 346 222 14 77 414 2 321 1.84e-17 83.2
MS.gene25415.t1 AT5G38570 31.119 286 161 12 139 414 116 375 9.71e-17 81.6
MS.gene25415.t1 AT1G05080 25.761 427 274 16 3 414 40 438 1.51e-16 81.6
MS.gene25415.t1 AT5G53840 24.350 423 279 17 2 412 44 437 1.94e-16 81.3
MS.gene25415.t1 AT4G10400 23.753 421 273 13 2 414 28 408 2.84e-16 80.5
MS.gene25415.t1 AT4G10400 23.753 421 273 13 2 414 28 408 2.84e-16 80.5
MS.gene25415.t1 AT4G10400 23.753 421 273 13 2 414 28 408 2.84e-16 80.5
MS.gene25415.t1 AT5G38590 25.758 396 237 17 2 380 28 383 8.18e-16 79.3
MS.gene25415.t1 AT5G22720 25.942 451 261 21 2 414 49 464 1.03e-15 79.3
MS.gene25415.t1 AT5G56400 25.866 433 265 18 2 415 60 455 1.40e-15 79.0
MS.gene25415.t1 AT3G50710 24.598 435 270 16 3 415 29 427 6.32e-15 76.6
MS.gene25415.t1 AT5G53635 23.853 436 271 16 3 414 27 425 8.80e-15 76.3
MS.gene25415.t1 AT5G56390 25.909 440 253 18 3 414 30 424 9.28e-15 76.3
MS.gene25415.t1 AT2G04230 27.431 401 240 15 2 381 39 409 1.02e-14 76.3
MS.gene25415.t1 AT3G54910 26.108 406 237 16 30 415 11 373 1.07e-14 75.5
MS.gene25415.t1 AT3G54910 26.108 406 237 16 30 415 11 373 1.07e-14 75.5
MS.gene25415.t1 AT3G54910 26.108 406 237 16 30 415 11 373 1.07e-14 75.5
MS.gene25415.t1 AT3G26920 28.032 371 222 16 57 414 1 339 1.38e-14 75.1
MS.gene25415.t1 AT3G26920 28.032 371 222 16 57 414 1 339 1.38e-14 75.1
MS.gene25415.t1 AT4G14103 31.020 245 143 11 2 234 34 264 5.11e-14 73.6
MS.gene25415.t1 AT4G14103 31.020 245 143 11 2 234 34 264 5.11e-14 73.6
MS.gene25415.t1 AT4G14103 31.301 246 141 11 2 234 34 264 8.26e-14 73.2
MS.gene25415.t1 AT5G56820 27.108 332 188 15 101 412 126 423 1.32e-13 72.8
MS.gene25415.t1 AT5G22670 27.642 369 223 17 31 376 59 406 2.41e-13 72.0
MS.gene25415.t1 AT3G49480 26.462 325 190 11 80 381 114 412 2.61e-13 71.6
MS.gene25415.t1 AT1G13780 24.208 442 279 18 2 412 36 452 4.06e-13 71.2
MS.gene25415.t1 AT1G66290 25.057 439 258 23 2 412 55 450 4.78e-13 70.9
MS.gene25415.t1 AT1G66310 24.083 436 265 18 2 412 45 439 7.31e-13 70.5
MS.gene25415.t1 AT2G26860 26.733 404 238 19 2 381 28 397 7.57e-13 70.1
MS.gene25415.t1 AT2G26860 26.733 404 238 19 2 381 28 397 7.57e-13 70.1
MS.gene25415.t1 AT5G56410 24.819 415 245 16 2 381 29 411 8.69e-13 70.5
MS.gene25415.t1 AT1G22000 24.318 440 259 18 2 412 55 449 2.18e-12 68.9
MS.gene25415.t1 AT3G26930 25.740 439 261 19 1 415 46 443 3.25e-12 68.6
MS.gene25415.t1 AT3G26930 25.740 439 261 19 1 415 1 398 3.46e-12 68.2
MS.gene25415.t1 AT5G50270 25.369 406 256 17 3 391 30 405 6.94e-12 67.0
MS.gene25415.t1 AT5G27750 25.845 414 254 17 2 381 31 425 1.04e-11 67.0
MS.gene25415.t1 AT5G44980 25.686 401 245 20 2 378 30 401 1.34e-11 66.2
MS.gene25415.t1 AT5G44980 25.686 401 245 20 2 378 30 401 1.34e-11 66.2
MS.gene25415.t1 AT5G38590 28.926 242 147 12 2 234 28 253 2.20e-11 64.7
MS.gene25415.t1 AT3G49480 26.460 291 169 11 110 381 8 272 2.57e-11 64.7
MS.gene25415.t1 AT3G26922 31.365 271 147 13 2 259 40 284 3.15e-11 64.3
MS.gene25415.t1 AT3G26922 31.365 271 147 13 2 259 40 284 3.15e-11 64.3
MS.gene25415.t1 AT3G26922 31.365 271 147 13 2 259 11 255 3.17e-11 63.9
MS.gene25415.t1 AT3G26922 31.365 271 147 13 2 259 11 255 3.17e-11 63.9
MS.gene25415.t1 AT3G26922 34.615 208 108 10 65 259 67 259 3.68e-11 63.9
MS.gene25415.t1 AT5G44950 24.939 413 265 17 2 393 30 418 4.97e-11 64.7
MS.gene25415.t1 AT5G56810 30.247 324 173 15 113 414 143 435 9.13e-11 63.9
MS.gene25415.t1 AT5G22610 23.214 448 281 18 2 413 44 464 9.42e-11 63.9

Find 90 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACCTATATTCACTTTAA+AGG 0.147428 8.2:+40455378 MS.gene25415:CDS
TTGAAGACAATGCTTATTTC+TGG 0.178189 8.2:+40455462 MS.gene25415:CDS
TTTGGCAATGCAACTTCTAT+TGG 0.243149 8.2:+40454787 MS.gene25415:CDS
GAGGACGCCGTTGACGAATA+TGG 0.282809 8.2:-40454084 None:intergenic
CGTCTCTCTTGCGGCCAATA+TGG 0.307804 8.2:+40454140 MS.gene25415:CDS
AGTTCCAAAGTTTCTAAGAT+TGG 0.318329 8.2:-40454622 None:intergenic
TACCAAAAGAAGAGATTATT+AGG 0.340479 8.2:+40455046 MS.gene25415:intron
ATTCACTTTAAAGGATATAT+AGG 0.344348 8.2:+40455387 MS.gene25415:CDS
TGGTGAAATATTTGTGGAAT+TGG 0.346093 8.2:+40454501 MS.gene25415:CDS
AAGACGCTACAACTAGAAAT+TGG 0.357484 8.2:+40454556 MS.gene25415:CDS
TTGGCAATGCAACTTCTATT+GGG 0.363130 8.2:+40454788 MS.gene25415:CDS
TTCGGCCTCTCTATCGCAAT+CGG 0.367347 8.2:-40454045 None:intergenic
AAGTGGGATACTAGACAATT+AGG 0.367479 8.2:-40455357 None:intergenic
TGCATTGCCAAATGTGATAT+TGG 0.374879 8.2:-40454776 None:intergenic
GTTAACGTACACAGTCTCTA+TGG 0.377657 8.2:+40454481 MS.gene25415:intron
CCGAATCTTTGGCCAAGCTT+AGG 0.388638 8.2:+40454656 MS.gene25415:CDS
CGAATCTTTGGCCAAGCTTA+GGG 0.393635 8.2:+40454657 MS.gene25415:CDS
AGCCTCACCAATATCACATT+TGG 0.403388 8.2:+40454769 MS.gene25415:CDS
TGCGAAGTACCCGCGACATT+CGG 0.408630 8.2:+40454111 MS.gene25415:CDS
TCGGCCTCTCTATCGCAATC+GGG 0.418289 8.2:-40454044 None:intergenic
CGTGGATCCGCACCGCCGTT+GGG 0.424276 8.2:+40454195 MS.gene25415:CDS
CTCACAGGTATCTTCATCTC+TGG 0.428747 8.2:+40453982 MS.gene25415:CDS
ACTCTCCTCCAAGCTCTCTC+TGG 0.432658 8.2:+40454886 MS.gene25415:CDS
GTTTAATGCCAGAGAGAGCT+TGG 0.438724 8.2:-40454894 None:intergenic
CCTAAGCTTGGCCAAAGATT+CGG 0.439078 8.2:-40454656 None:intergenic
TGAAGAAATCAAGTCCATAT+TGG 0.439919 8.2:-40454154 None:intergenic
GGCCAAAGATTCGGTTGAAA+AGG 0.442677 8.2:-40454647 None:intergenic
AGAGGAGGTTAGTGCATGAT+AGG 0.446173 8.2:-40454289 None:intergenic
TGAAGCACGTCGTACAGAAA+AGG 0.458324 8.2:-40455157 None:intergenic
CTGTCCAATCTTAGAAACTT+TGG 0.459587 8.2:+40454618 MS.gene25415:CDS
CTTGGAGGAGAGTGAGTAAA+GGG 0.461485 8.2:-40454876 None:intergenic
GCACTAACCTCCTCTCCATC+AGG 0.467643 8.2:+40454297 MS.gene25415:CDS
CTCGCTCTCTTCTCGTCTGA+GGG 0.467717 8.2:+40454239 MS.gene25415:CDS
AGTTGTAGCGTCTTCAATGA+TGG 0.478034 8.2:-40454547 None:intergenic
ATTATGAGAGCTTGAAGTAT+AGG 0.479156 8.2:-40455187 None:intergenic
ACGTGGATCCGCACCGCCGT+TGG 0.482549 8.2:+40454194 MS.gene25415:CDS
TCTCTATGGTGAAATATTTG+TGG 0.484639 8.2:+40454495 MS.gene25415:CDS
ATATCCTTTAAAGTGAATAT+AGG 0.491831 8.2:-40455382 None:intergenic
CATCGTAGAAATCAAAAGCT+TGG 0.492563 8.2:-40454013 None:intergenic
TCTCGCTCTCTTCTCGTCTG+AGG 0.498144 8.2:+40454238 MS.gene25415:CDS
CAAAGTTTCTAAGATTGGAC+AGG 0.500334 8.2:-40454617 None:intergenic
ATGTGATATTGGTGAGGCTA+AGG 0.505330 8.2:-40454765 None:intergenic
TAACTTAGTCAAATTTAACT+TGG 0.510908 8.2:-40455569 None:intergenic
ATCCTCAAGGTGGGGCCCAA+CGG 0.512786 8.2:-40454210 None:intergenic
GAAGCACGTCGTACAGAAAA+GGG 0.514480 8.2:-40455156 None:intergenic
CAATGAGTCTTGTTTCTCAC+AGG 0.521437 8.2:+40453967 None:intergenic
AGGTTAGTGCATGATAGGAC+CGG 0.527359 8.2:-40454284 None:intergenic
CTCAAGGTGGGGCCCAACGG+CGG 0.527894 8.2:-40454207 None:intergenic
CGGCTTCAAACTCCCTCTCC+CGG 0.528625 8.2:+40454265 MS.gene25415:CDS
CGCCGTTGGGCCCCACCTTG+AGG 0.530412 8.2:+40454208 MS.gene25415:CDS
GCTTGGAGGAGAGTGAGTAA+AGG 0.536107 8.2:-40454877 None:intergenic
TAAAGTGAATATAGGTCAAG+TGG 0.551062 8.2:-40455374 None:intergenic
AACAGATTCACTTTCAGAAG+AGG 0.560957 8.2:-40454851 None:intergenic
GTTTCGTTGTTATGCAGCAA+AGG 0.562730 8.2:+40454924 MS.gene25415:CDS
GGTGGGGCCCAACGGCGGTG+CGG 0.567700 8.2:-40454202 None:intergenic
ACGCATCTTCCGAATGTCGC+GGG 0.569466 8.2:-40454120 None:intergenic
ACAACTAGAAATTGGTAATG+TGG 0.572331 8.2:+40454564 MS.gene25415:CDS
TTAAAGGATATATAGGACAT+TGG 0.572576 8.2:+40455394 MS.gene25415:CDS
CTCTTCTCGTCTGAGGGTGA+CGG 0.572937 8.2:+40454245 MS.gene25415:CDS
AGATTGGACAGGCAGAGAGA+AGG 0.579911 8.2:-40454606 None:intergenic
GGAAGATGGAACCCTAAGCT+TGG 0.579963 8.2:-40454668 None:intergenic
GTGCATGATAGGACCGGGAG+AGG 0.587136 8.2:-40454278 None:intergenic
TAATGCCAGAGAGAGCTTGG+AGG 0.588199 8.2:-40454891 None:intergenic
GACTTTGCCATATTCGTCAA+CGG 0.590231 8.2:+40454077 MS.gene25415:CDS
TTTGTTACCTGATGGAGAGG+AGG 0.590784 8.2:-40454304 None:intergenic
TGTCTTGAGATTGACACACC+TGG 0.595030 8.2:+40454733 MS.gene25415:CDS
CGGCCTCTCTATCGCAATCG+GGG 0.599475 8.2:-40454043 None:intergenic
CGCGGGTACTTCGCATAGAG+AGG 0.601043 8.2:-40454103 None:intergenic
AGATTTGTTACCTGATGGAG+AGG 0.603999 8.2:-40454307 None:intergenic
GAGATGAAGATCCTCAAGGT+GGG 0.607880 8.2:-40454219 None:intergenic
TGCATGATAGGACCGGGAGA+GGG 0.607937 8.2:-40454277 None:intergenic
CGGAAGATGCGTCTCTCTTG+CGG 0.610368 8.2:+40454131 MS.gene25415:CDS
TGGCAGACAGTAGATCCCCT+TGG 0.612624 8.2:-40455549 None:intergenic
GGTTAGTGCATGATAGGACC+GGG 0.613486 8.2:-40454283 None:intergenic
CTGAACGCTCTGTTGACACG+TGG 0.616550 8.2:+40454177 MS.gene25415:CDS
AGGCTAAGGTACTTGAGACC+AGG 0.622331 8.2:-40454751 None:intergenic
TACAGAAAAGGGTCGACAGT+GGG 0.624804 8.2:-40455145 None:intergenic
ACGAGTTAAATATAGTCTCA+CGG 0.631410 8.2:+40455317 MS.gene25415:CDS
AGATGAAGATCCTCAAGGTG+GGG 0.631714 8.2:-40454218 None:intergenic
AAAGTGAATATAGGTCAAGT+GGG 0.636598 8.2:-40455373 None:intergenic
GACGCATCTTCCGAATGTCG+CGG 0.645697 8.2:-40454121 None:intergenic
CGAGATGAAGATCCTCAAGG+TGG 0.647298 8.2:-40454220 None:intergenic
GAGCGAGATGAAGATCCTCA+AGG 0.648810 8.2:-40454223 None:intergenic
TTACCCCGATTGCGATAGAG+AGG 0.653960 8.2:+40454040 MS.gene25415:CDS
GGCAGAGAGAAGGATATCAA+CGG 0.662736 8.2:-40454596 None:intergenic
GTACAGAAAAGGGTCGACAG+TGG 0.663564 8.2:-40455146 None:intergenic
TATTGGGAACTTGCACAACG+TGG 0.670170 8.2:+40454804 MS.gene25415:CDS
TGGGAACTTGCACAACGTGG+AGG 0.687506 8.2:+40454807 MS.gene25415:CDS
TGCCAAATGTGATATTGGTG+AGG 0.688345 8.2:-40454771 None:intergenic
TCTCATAATTCATAATGACA+AGG 0.702264 8.2:+40455201 MS.gene25415:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTAAATTTAAAACTTTTCA+AGG - chr8.2:40454692-40454711 None:intergenic 15.0%
!!! ATTTTCGAAAAATCTATAAT+AGG - chr8.2:40455079-40455098 None:intergenic 15.0%
!! AAAAAGGTAAAGATTGAAAA+TGG - chr8.2:40454396-40454415 None:intergenic 20.0%
!! AACTATAAAAAGTGATGAAA+GGG - chr8.2:40454340-40454359 None:intergenic 20.0%
!! AATGGAATATATAATACACA+AGG - chr8.2:40454969-40454988 None:intergenic 20.0%
!! CAAATAATAACTGTACAAAA+AGG - chr8.2:40454412-40454431 None:intergenic 20.0%
!!! ATATCCTTTAAAGTGAATAT+AGG - chr8.2:40455385-40455404 None:intergenic 20.0%
!!! ATTCACTTTAAAGGATATAT+AGG + chr8.2:40455387-40455406 MS.gene25415:CDS 20.0%
!!! TGTGTTTTTGTTTGATTATT+TGG + chr8.2:40455262-40455281 MS.gene25415:intron 20.0%
! CAACTATAAAAAGTGATGAA+AGG - chr8.2:40454341-40454360 None:intergenic 25.0%
! TACCAAAAGAAGAGATTATT+AGG + chr8.2:40455046-40455065 MS.gene25415:intron 25.0%
! TCTCATAATTCATAATGACA+AGG + chr8.2:40455201-40455220 MS.gene25415:CDS 25.0%
! TTAAAGGATATATAGGACAT+TGG + chr8.2:40455394-40455413 MS.gene25415:CDS 25.0%
! TTGACCTATATTCACTTTAA+AGG + chr8.2:40455378-40455397 MS.gene25415:CDS 25.0%
!! AAAATTTTCGAATATGCCAA+GGG + chr8.2:40455533-40455552 MS.gene25415:CDS 25.0%
!! TACCTAATAATCTCTTCTTT+TGG - chr8.2:40455051-40455070 None:intergenic 25.0%
!! TTTAACTTAAGCTTTAGTGT+GGG + chr8.2:40455237-40455256 MS.gene25415:intron 25.0%
!!! TTAAAACTTTTCAAGGAAGA+TGG - chr8.2:40454685-40454704 None:intergenic 25.0%
!!! TTTTTCGAAAATGCACTTTT+CGG + chr8.2:40455087-40455106 MS.gene25415:CDS 25.0%
AAAGTGAATATAGGTCAAGT+GGG - chr8.2:40455376-40455395 None:intergenic 30.0%
ACGAGTTAAATATAGTCTCA+CGG + chr8.2:40455317-40455336 MS.gene25415:CDS 30.0%
ATTATGAGAGCTTGAAGTAT+AGG - chr8.2:40455190-40455209 None:intergenic 30.0%
TAAAGTGAATATAGGTCAAG+TGG - chr8.2:40455377-40455396 None:intergenic 30.0%
TCTCTATGGTGAAATATTTG+TGG + chr8.2:40454495-40454514 MS.gene25415:CDS 30.0%
TGAAGAAATCAAGTCCATAT+TGG - chr8.2:40454157-40454176 None:intergenic 30.0%
TGGTGAAATATTTGTGGAAT+TGG + chr8.2:40454501-40454520 MS.gene25415:CDS 30.0%
TTGAAGACAATGCTTATTTC+TGG + chr8.2:40455462-40455481 MS.gene25415:CDS 30.0%
! AAATTTTCGAATATGCCAAG+GGG + chr8.2:40455534-40455553 MS.gene25415:CDS 30.0%
! AGACAATTAGGAACACTTTT+CGG - chr8.2:40455348-40455367 None:intergenic 30.0%
! GAAAATTTTCGAATATGCCA+AGG + chr8.2:40455532-40455551 MS.gene25415:CDS 30.0%
! GTTTAACTTAAGCTTTAGTG+TGG + chr8.2:40455236-40455255 MS.gene25415:intron 30.0%
!! ACAAAAGATTTGTTACCTGA+TGG - chr8.2:40454315-40454334 None:intergenic 30.0%
!! ACAACTAGAAATTGGTAATG+TGG + chr8.2:40454564-40454583 MS.gene25415:CDS 30.0%
!! AGTTCCAAAGTTTCTAAGAT+TGG - chr8.2:40454625-40454644 None:intergenic 30.0%
!! AGTTGTAATTTGTTTCCTAC+AGG + chr8.2:40455287-40455306 MS.gene25415:intron 30.0%
!!! GGCAAAGTCTTTGAAATTTT+CGG - chr8.2:40454066-40454085 None:intergenic 30.0%
AACAGATTCACTTTCAGAAG+AGG - chr8.2:40454854-40454873 None:intergenic 35.0%
AAGACGCTACAACTAGAAAT+TGG + chr8.2:40454556-40454575 MS.gene25415:CDS 35.0%
AAGTGGGATACTAGACAATT+AGG - chr8.2:40455360-40455379 None:intergenic 35.0%
CATCGTAGAAATCAAAAGCT+TGG - chr8.2:40454016-40454035 None:intergenic 35.0%
CTGTCCAATCTTAGAAACTT+TGG + chr8.2:40454618-40454637 MS.gene25415:CDS 35.0%
TGCATTGCCAAATGTGATAT+TGG - chr8.2:40454779-40454798 None:intergenic 35.0%
TTGGCAATGCAACTTCTATT+GGG + chr8.2:40454788-40454807 MS.gene25415:CDS 35.0%
TTTGGCAATGCAACTTCTAT+TGG + chr8.2:40454787-40454806 MS.gene25415:CDS 35.0%
! GCTAGTTATGTTTTGCAGTA+TGG + chr8.2:40455432-40455451 MS.gene25415:CDS 35.0%
! TTCCTTTTCAACCGAATCTT+TGG + chr8.2:40454645-40454664 MS.gene25415:CDS 35.0%
!! CAAAGTTTCTAAGATTGGAC+AGG - chr8.2:40454620-40454639 None:intergenic 35.0%
AGCCTCACCAATATCACATT+TGG + chr8.2:40454769-40454788 MS.gene25415:CDS 40.0%
AGTTGTAGCGTCTTCAATGA+TGG - chr8.2:40454550-40454569 None:intergenic 40.0%
ATGTGATATTGGTGAGGCTA+AGG - chr8.2:40454768-40454787 None:intergenic 40.0%
CTAATTCTCGTATCTCCACT+TGG + chr8.2:40455009-40455028 MS.gene25415:intron 40.0%
GACTTTGCCATATTCGTCAA+CGG + chr8.2:40454077-40454096 MS.gene25415:CDS 40.0%
GTTAACGTACACAGTCTCTA+TGG + chr8.2:40454481-40454500 MS.gene25415:intron 40.0%
GTTTCGTTGTTATGCAGCAA+AGG + chr8.2:40454924-40454943 MS.gene25415:CDS 40.0%
TAACTCGTGATGTATCCTGT+AGG - chr8.2:40455305-40455324 None:intergenic 40.0%
TGCCAAATGTGATATTGGTG+AGG - chr8.2:40454774-40454793 None:intergenic 40.0%
!! AGATTTGTTACCTGATGGAG+AGG - chr8.2:40454310-40454329 None:intergenic 40.0%
ACGTACACAACAACACCAAG+TGG - chr8.2:40455027-40455046 None:intergenic 45.0%
AGAGGAGGTTAGTGCATGAT+AGG - chr8.2:40454292-40454311 None:intergenic 45.0%
AGATGAAGATCCTCAAGGTG+GGG - chr8.2:40454221-40454240 None:intergenic 45.0%
AGGTTAGTGCATGATAGGAC+CGG - chr8.2:40454287-40454306 None:intergenic 45.0%
CCTAAGCTTGGCCAAAGATT+CGG - chr8.2:40454659-40454678 None:intergenic 45.0%
CGAATCTTTGGCCAAGCTTA+GGG + chr8.2:40454657-40454676 MS.gene25415:CDS 45.0%
CTCACAGGTATCTTCATCTC+TGG + chr8.2:40453982-40454001 MS.gene25415:CDS 45.0%
CTTGGAGGAGAGTGAGTAAA+GGG - chr8.2:40454879-40454898 None:intergenic 45.0%
GAAGCACGTCGTACAGAAAA+GGG - chr8.2:40455159-40455178 None:intergenic 45.0%
GAGATGAAGATCCTCAAGGT+GGG - chr8.2:40454222-40454241 None:intergenic 45.0%
GGCAGAGAGAAGGATATCAA+CGG - chr8.2:40454599-40454618 None:intergenic 45.0%
GTTTAATGCCAGAGAGAGCT+TGG - chr8.2:40454897-40454916 None:intergenic 45.0%
TACAGAAAAGGGTCGACAGT+GGG - chr8.2:40455148-40455167 None:intergenic 45.0%
TATTGGGAACTTGCACAACG+TGG + chr8.2:40454804-40454823 MS.gene25415:CDS 45.0%
TGAAGCACGTCGTACAGAAA+AGG - chr8.2:40455160-40455179 None:intergenic 45.0%
TGTCTTGAGATTGACACACC+TGG + chr8.2:40454733-40454752 MS.gene25415:CDS 45.0%
TTTGTTACCTGATGGAGAGG+AGG - chr8.2:40454307-40454326 None:intergenic 45.0%
! GGCCAAAGATTCGGTTGAAA+AGG - chr8.2:40454650-40454669 None:intergenic 45.0%
!!! CAAATTTTTTAAATATATAA+TGG - chr8.2:40454987-40455006 None:intergenic 5.0%
AGATTGGACAGGCAGAGAGA+AGG - chr8.2:40454609-40454628 None:intergenic 50.0%
CCGAATCTTTGGCCAAGCTT+AGG + chr8.2:40454656-40454675 MS.gene25415:CDS 50.0%
CGAGATGAAGATCCTCAAGG+TGG - chr8.2:40454223-40454242 None:intergenic 50.0%
GAGCGAGATGAAGATCCTCA+AGG - chr8.2:40454226-40454245 None:intergenic 50.0%
GCTTGGAGGAGAGTGAGTAA+AGG - chr8.2:40454880-40454899 None:intergenic 50.0%
GGAAGATGGAACCCTAAGCT+TGG - chr8.2:40454671-40454690 None:intergenic 50.0%
GGTTAGTGCATGATAGGACC+GGG - chr8.2:40454286-40454305 None:intergenic 50.0%
GTACAGAAAAGGGTCGACAG+TGG - chr8.2:40455149-40455168 None:intergenic 50.0%
TAATGCCAGAGAGAGCTTGG+AGG - chr8.2:40454894-40454913 None:intergenic 50.0%
TTACCCCGATTGCGATAGAG+AGG + chr8.2:40454040-40454059 MS.gene25415:CDS 50.0%
TTCGGCCTCTCTATCGCAAT+CGG - chr8.2:40454048-40454067 None:intergenic 50.0%
! AGGCTAAGGTACTTGAGACC+AGG - chr8.2:40454754-40454773 None:intergenic 50.0%
ACGCATCTTCCGAATGTCGC+GGG - chr8.2:40454123-40454142 None:intergenic 55.0%
ACTCTCCTCCAAGCTCTCTC+TGG + chr8.2:40454886-40454905 MS.gene25415:CDS 55.0%
CGGAAGATGCGTCTCTCTTG+CGG + chr8.2:40454131-40454150 MS.gene25415:CDS 55.0%
CGTCTCTCTTGCGGCCAATA+TGG + chr8.2:40454140-40454159 MS.gene25415:CDS 55.0%
CTCGCTCTCTTCTCGTCTGA+GGG + chr8.2:40454239-40454258 MS.gene25415:CDS 55.0%
CTCTTCTCGTCTGAGGGTGA+CGG + chr8.2:40454245-40454264 MS.gene25415:CDS 55.0%
CTGAACGCTCTGTTGACACG+TGG + chr8.2:40454177-40454196 MS.gene25415:CDS 55.0%
GACGCATCTTCCGAATGTCG+CGG - chr8.2:40454124-40454143 None:intergenic 55.0%
GAGGACGCCGTTGACGAATA+TGG - chr8.2:40454087-40454106 None:intergenic 55.0%
GCACTAACCTCCTCTCCATC+AGG + chr8.2:40454297-40454316 MS.gene25415:CDS 55.0%
TCGGCCTCTCTATCGCAATC+GGG - chr8.2:40454047-40454066 None:intergenic 55.0%
TCTCGCTCTCTTCTCGTCTG+AGG + chr8.2:40454238-40454257 MS.gene25415:CDS 55.0%
TGCATGATAGGACCGGGAGA+GGG - chr8.2:40454280-40454299 None:intergenic 55.0%
TGGCAGACAGTAGATCCCCT+TGG - chr8.2:40455552-40455571 None:intergenic 55.0%
TGGGAACTTGCACAACGTGG+AGG + chr8.2:40454807-40454826 MS.gene25415:CDS 55.0%
! TGCGAAGTACCCGCGACATT+CGG + chr8.2:40454111-40454130 MS.gene25415:CDS 55.0%
ATCCTCAAGGTGGGGCCCAA+CGG - chr8.2:40454213-40454232 None:intergenic 60.0%
CGCGGGTACTTCGCATAGAG+AGG - chr8.2:40454106-40454125 None:intergenic 60.0%
CGGCCTCTCTATCGCAATCG+GGG - chr8.2:40454046-40454065 None:intergenic 60.0%
CGGCTTCAAACTCCCTCTCC+CGG + chr8.2:40454265-40454284 MS.gene25415:CDS 60.0%
GTGCATGATAGGACCGGGAG+AGG - chr8.2:40454281-40454300 None:intergenic 60.0%
ACGTGGATCCGCACCGCCGT+TGG + chr8.2:40454194-40454213 MS.gene25415:CDS 70.0%
CGTGGATCCGCACCGCCGTT+GGG + chr8.2:40454195-40454214 MS.gene25415:CDS 70.0%
CTCAAGGTGGGGCCCAACGG+CGG - chr8.2:40454210-40454229 None:intergenic 70.0%
CGCCGTTGGGCCCCACCTTG+AGG + chr8.2:40454208-40454227 MS.gene25415:CDS 75.0%
! GGTGGGGCCCAACGGCGGTG+CGG - chr8.2:40454205-40454224 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 40453969 40455587 40453969 ID=MS.gene25415
chr8.2 mRNA 40453969 40455587 40453969 ID=MS.gene25415.t1;Parent=MS.gene25415
chr8.2 exon 40453969 40454318 40453969 ID=MS.gene25415.t1.exon1;Parent=MS.gene25415.t1
chr8.2 CDS 40453969 40454318 40453969 ID=cds.MS.gene25415.t1;Parent=MS.gene25415.t1
chr8.2 exon 40454495 40454945 40454495 ID=MS.gene25415.t1.exon2;Parent=MS.gene25415.t1
chr8.2 CDS 40454495 40454945 40454495 ID=cds.MS.gene25415.t1;Parent=MS.gene25415.t1
chr8.2 exon 40455055 40455222 40455055 ID=MS.gene25415.t1.exon3;Parent=MS.gene25415.t1
chr8.2 CDS 40455055 40455222 40455055 ID=cds.MS.gene25415.t1;Parent=MS.gene25415.t1
chr8.2 exon 40455309 40455587 40455309 ID=MS.gene25415.t1.exon4;Parent=MS.gene25415.t1
chr8.2 CDS 40455309 40455587 40455309 ID=cds.MS.gene25415.t1;Parent=MS.gene25415.t1
Gene Sequence

>MS.gene25415

ATGAGTCTTGTTTCTCACAGGTATCTTCATCTCTGGAAACATCTCCAAGCTTTTGATTTCTACGATGTGTATTACCCCGATTGCGATAGAGAGGCCGAAAATTTCAAAGACTTTGCCATATTCGTCAACGGCGTCCTCTCTATGCGAAGTACCCGCGACATTCGGAAGATGCGTCTCTCTTGCGGCCAATATGGACTTGATTTCTTCACTGAACGCTCTGTTGACACGTGGATCCGCACCGCCGTTGGGCCCCACCTTGAGGATCTTCATCTCGCTCTCTTCTCGTCTGAGGGTGACGGCTTCAAACTCCCTCTCCCGGTCCTATCATGCACTAACCTCCTCTCCATCAGGTAACAAATCTTTTGTAACCCTTTCATCACTTTTTATAGTTGTAACGTTTGTCAAACAATCTTCTATTTGCATGCCATTTTCAATCTTTACCTTTTTGTACAGTTATTATTTGCACAGTTATTCTTGTTTAAATTAAAATGTGACATAATAAATGAAGATTTGTTAACGTACACAGTCTCTATGGTGAAATATTTGTGGAATTGGAACAATCTTCGCGTGTTTGTTTACCATCATTGAAGACGCTACAACTAGAAATTGGTAATGTGGATGTGAATTCCGTTGATATCCTTCTCTCTGCCTGTCCAATCTTAGAAACTTTGGAACTTTCCTTTTCAACCGAATCTTTGGCCAAGCTTAGGGTTCCATCTTCCTTGAAAAGTTTTAAATTTACTATAGAGAATGACACTGAAGTTTGTCTTGAGATTGACACACCTGGTCTCAAGTACCTTAGCCTCACCAATATCACATTTGGCAATGCAACTTCTATTGGGAACTTGCACAACGTGGAGGAAGCATATCTTGATGTACTCTCCTCTTCTGAAAGTGAATCTGTTGACCCTTTACTCACTCTCCTCCAAGCTCTCTCTGGCATTAAACGTCTAGTGTTTCGTTGTTATGCAGCAAAGGTGAGTGAAATTTTCATTATCCTTGTGTATTATATATTCCATTATATATTTAAAAAATTTGTGCTAATTCTCGTATCTCCACTTGGTGTTGTTGTGTACGTACCAAAAGAAGAGATTATTAGGTACTGAACCTATTATAGATTTTTCGAAAATGCACTTTTCGGAATTTCGCTGTTTACTACATCTAGAGCTCATTTTTCCCACTGTCGACCCTTTTCTGTACGACGTGCTTCAGAAATGTCCTATACTTCAAGCTCTCATAATTCATAATGACAAGGTTCGTAAATAGAGTTTAACTTAAGCTTTAGTGTGGGTGTGTGTTTTTGTTTGATTATTTGGTGAGTTGTAATTTGTTTCCTACAGGATACATCACGAGTTAAATATAGTCTCACGGAAAAGCCGAAAAGTGTTCCTAATTGTCTAGTATCCCACTTGACCTATATTCACTTTAAAGGATATATAGGACATTGGCATGAGAAAGAATTTGCTAGTTATGTTTTGCAGTATGGACTTGTTTTGAAGACAATGCTTATTTCTGGTTTTTTGTCGAATGAAACGAAGAAGCATGCTAAGTACAACTATCGCAGAAAATTTTCGAATATGCCAAGGGGATCTACTGTCTGCCAAGTTAAATTTGACTAA
Protein sequence

>MS.gene25415.t1

MSLVSHRYLHLWKHLQAFDFYDVYYPDCDREAENFKDFAIFVNGVLSMRSTRDIRKMRLSCGQYGLDFFTERSVDTWIRTAVGPHLEDLHLALFSSEGDGFKLPLPVLSCTNLLSISLYGEIFVELEQSSRVCLPSLKTLQLEIGNVDVNSVDILLSACPILETLELSFSTESLAKLRVPSSLKSFKFTIENDTEVCLEIDTPGLKYLSLTNITFGNATSIGNLHNVEEAYLDVLSSSESESVDPLLTLLQALSGIKRLVFRCYAAKKRLLGTEPIIDFSKMHFSEFRCLLHLELIFPTVDPFLYDVLQKCPILQALIIHNDKDTSRVKYSLTEKPKSVPNCLVSHLTYIHFKGYIGHWHEKEFASYVLQYGLVLKTMLISGFLSNETKKHAKYNYRRKFSNMPRGSTVCQVKFD