AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63260.t1 MTR_8g070950 66.749 403 128 5 1 402 41 438 1.18e-178 505
MS.gene63260.t1 MTR_8g467790 69.516 351 69 6 1 343 174 494 9.01e-159 457
MS.gene63260.t1 MTR_8g062010 60.636 409 145 8 1 402 35 434 1.01e-151 436
MS.gene63260.t1 MTR_8g062080 56.386 415 156 6 1 403 49 450 5.39e-150 432
MS.gene63260.t1 MTR_8g062310 56.824 403 146 8 4 402 54 432 2.17e-143 415
MS.gene63260.t1 MTR_8g058965 53.704 432 160 9 2 403 40 461 5.68e-137 400
MS.gene63260.t1 MTR_8g469020 51.270 433 168 8 2 402 34 455 7.36e-135 394
MS.gene63260.t1 MTR_8g062300 52.561 449 149 13 1 403 52 482 3.43e-133 391
MS.gene63260.t1 MTR_4g050250 51.169 385 136 13 1 344 47 420 7.33e-107 322
MS.gene63260.t1 MTR_2g083840 42.280 421 206 14 3 398 49 457 2.14e-78 249
MS.gene63260.t1 MTR_8g062270 37.471 427 223 15 1 397 41 453 3.14e-73 236
MS.gene63260.t1 MTR_8g058970 38.652 445 214 20 1 398 45 477 3.05e-70 229
MS.gene63260.t1 MTR_8g468810 37.939 427 222 17 1 398 51 463 1.53e-68 224
MS.gene63260.t1 MTR_2g086020 73.973 146 38 0 257 402 174 319 4.97e-68 218
MS.gene63260.t1 MTR_2g086020 44.898 147 61 3 1 137 1 137 1.47e-28 114
MS.gene63260.t1 MTR_5g092960 36.235 425 231 11 3 397 47 461 1.09e-65 216
MS.gene63260.t1 MTR_8g014260 37.647 425 218 19 4 398 44 451 4.55e-65 214
MS.gene63260.t1 MTR_8g468840 38.095 420 224 15 2 396 40 448 3.54e-62 207
MS.gene63260.t1 MTR_8g467800 39.486 428 216 15 2 402 46 457 4.32e-62 207
MS.gene63260.t1 MTR_8g468760 35.294 425 207 12 1 397 34 418 3.38e-59 198
MS.gene63260.t1 MTR_4g032730 33.945 436 235 12 3 397 43 466 5.57e-59 199
MS.gene63260.t1 MTR_8g445780 38.503 374 180 18 4 351 41 390 9.39e-54 183
MS.gene63260.t1 MTR_8g014160 33.890 419 195 17 2 398 67 425 7.52e-48 169
MS.gene63260.t1 MTR_5g092960 37.349 332 175 9 3 308 47 371 7.61e-46 162
MS.gene63260.t1 MTR_8g468810 37.612 335 168 16 1 308 51 371 7.05e-45 159
MS.gene63260.t1 MTR_8g468810 39.608 255 124 12 1 235 51 295 1.07e-36 136
MS.gene63260.t1 MTR_8g468810 39.608 255 124 12 1 235 51 295 1.78e-36 136
MS.gene63260.t1 MTR_8g468810 39.608 255 124 12 1 235 51 295 2.88e-36 136
MS.gene63260.t1 MTR_4g024710 35.410 305 163 13 113 398 58 347 5.02e-36 135
MS.gene63260.t1 MTR_8g014210 39.834 241 124 9 169 398 1 231 4.09e-34 127
MS.gene63260.t1 MTR_8g077460 48.120 133 66 3 266 398 246 375 1.99e-30 120
MS.gene63260.t1 MTR_8g077460 37.766 188 98 9 4 177 38 220 2.23e-21 95.1
MS.gene63260.t1 MTR_8g467800 39.568 278 135 12 2 259 46 310 6.12e-29 115
MS.gene63260.t1 MTR_3g099800 62.069 87 32 1 310 395 19 105 1.37e-27 105
MS.gene63260.t1 MTR_7g032370 27.251 411 211 14 2 398 16 352 6.54e-26 107
MS.gene63260.t1 MTR_4g024745 33.448 290 159 13 111 381 10 284 1.44e-24 102
MS.gene63260.t1 MTR_1g041245 25.598 418 222 16 2 398 37 386 2.19e-18 86.7
MS.gene63260.t1 MTR_5g021550 43.333 120 53 7 4 112 47 162 5.26e-18 81.3
MS.gene63260.t1 MTR_8g014290 38.710 124 61 6 4 113 38 160 4.71e-17 78.6
MS.gene63260.t1 MTR_8g014230 41.667 84 47 2 308 391 17 98 2.98e-16 74.3
MS.gene63260.t1 MTR_3g108150 24.752 404 240 16 2 397 39 386 3.84e-16 79.7
MS.gene63260.t1 MTR_7g098510 24.878 410 233 15 2 398 44 391 1.64e-15 78.2
MS.gene63260.t1 MTR_7g034115 26.471 408 212 18 2 390 37 375 5.35e-15 76.3
MS.gene63260.t1 MTR_3g071850 25.654 382 207 14 2 363 50 374 1.25e-14 76.3
MS.gene63260.t1 MTR_7g032380 34.682 173 81 9 2 159 37 192 5.27e-14 73.2
MS.gene63260.t1 MTR_6g022500 25.118 422 213 19 5 398 130 476 6.04e-14 73.6
MS.gene63260.t1 MTR_5g084070 24.750 400 271 13 2 390 39 419 6.42e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63260.t1 AT5G56560 27.381 420 247 21 3 398 33 418 1.14e-17 84.7
MS.gene63260.t1 AT1G78750 26.047 430 264 19 2 395 44 455 9.79e-16 79.0
MS.gene63260.t1 AT1G78750 26.047 430 264 19 2 395 44 455 9.79e-16 79.0
MS.gene63260.t1 AT2G26030 26.056 426 266 19 2 395 30 438 1.23e-14 75.9
MS.gene63260.t1 AT2G26030 26.056 426 266 19 2 395 30 438 1.23e-14 75.9
MS.gene63260.t1 AT1G69630 26.218 431 247 18 2 388 44 447 2.03e-14 75.1
MS.gene63260.t1 AT2G26030 26.037 434 261 20 2 395 30 443 1.39e-13 72.4
MS.gene63260.t1 AT3G59210 28.896 308 182 15 2 286 33 326 2.05e-13 72.0
MS.gene63260.t1 AT3G59210 28.896 308 182 15 2 286 33 326 2.05e-13 72.0
MS.gene63260.t1 AT3G59210 28.896 308 182 15 2 286 33 326 2.05e-13 72.0
MS.gene63260.t1 AT3G59210 28.896 308 182 15 2 286 33 326 2.05e-13 72.0
MS.gene63260.t1 AT3G59210 28.896 308 182 15 2 286 33 326 2.05e-13 72.0
MS.gene63260.t1 AT4G26340 26.714 423 253 20 2 398 28 419 5.16e-13 70.5
MS.gene63260.t1 AT5G50270 26.290 407 243 17 3 380 30 408 6.86e-13 70.1
MS.gene63260.t1 AT4G10400 25.598 418 252 15 2 397 28 408 1.73e-12 68.9
MS.gene63260.t1 AT4G10400 25.598 418 252 15 2 397 28 408 1.73e-12 68.9
MS.gene63260.t1 AT4G10400 25.598 418 252 15 2 397 28 408 1.73e-12 68.9
MS.gene63260.t1 AT3G59200 32.530 166 98 6 2 158 33 193 2.01e-12 68.6
MS.gene63260.t1 AT3G54910 26.720 378 230 13 42 398 22 373 1.28e-11 66.2
MS.gene63260.t1 AT3G54910 26.720 378 230 13 42 398 22 373 1.28e-11 66.2
MS.gene63260.t1 AT3G54910 26.720 378 230 13 42 398 22 373 1.28e-11 66.2
MS.gene63260.t1 AT4G13985 23.821 424 283 16 1 395 44 456 5.05e-11 64.7
MS.gene63260.t1 AT2G29930 24.944 449 256 19 2 397 38 458 6.96e-11 64.3
MS.gene63260.t1 AT2G29930 24.944 449 256 19 2 397 38 458 6.96e-11 64.3

Find 90 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATCCACTTATATTCTT+TGG 0.187661 8.4:-42622510 MS.gene63260:CDS
TTTGCATCATTCAACATTAA+AGG 0.190910 8.4:-42622162 MS.gene63260:intron
CTCGAAGGTGGCACCATCTT+TGG 0.209716 8.4:-42623818 MS.gene63260:CDS
TTACTTTGAGTTTGATGATA+TGG 0.233411 8.4:-42622336 MS.gene63260:CDS
TTTGCGAAACGGACTTGTTT+TGG 0.269271 8.4:-42621644 MS.gene63260:CDS
GTCAACAAACCTTGCTGCTT+TGG 0.276221 8.4:+42621906 None:intergenic
CCAAGTGAAATTGTCATTGA+TGG 0.289134 8.4:+42622361 None:intergenic
CCATCTTTGGGAGCAACTTC+AGG 0.299675 8.4:-42623805 MS.gene63260:CDS
CGTTGGCATCTTTCATAGTA+TGG 0.317999 8.4:-42622291 MS.gene63260:CDS
TTGAAAACACTGCAGTCTTA+AGG 0.321303 8.4:-42622591 MS.gene63260:intron
AGATTTGATGCACCTCTATA+CGG 0.322760 8.4:+42622188 None:intergenic
ACTTTAAACCTCTTCTCATC+TGG 0.333346 8.4:+42623639 None:intergenic
GACCCAGATGTATCTTCTTA+AGG 0.337539 8.4:-42621511 None:intergenic
TGAAAACACTGCAGTCTTAA+GGG 0.351089 8.4:-42622590 MS.gene63260:intron
TCGAAGGTGGCACCATCTTT+GGG 0.357870 8.4:-42623817 MS.gene63260:CDS
TCTCTCTGGCTGCCCTGTGC+TGG 0.359507 8.4:-42622471 MS.gene63260:CDS
TCAAAAGTTGCCTTTCTCTC+TGG 0.364635 8.4:-42622485 MS.gene63260:CDS
AACTCTGGATAGTTTAGAAC+AGG 0.380429 8.4:+42622028 None:intergenic
CTCGGATGCAGTCGAGAATA+GGG 0.385265 8.4:+42622219 None:intergenic
CACAGGGCAGCCAGAGAGAA+AGG 0.388272 8.4:+42622475 None:intergenic
CTGCCCTGTGCTGGAAACTT+TGG 0.398496 8.4:-42622462 MS.gene63260:CDS
TCTCGGATGCAGTCGAGAAT+AGG 0.404693 8.4:+42622218 None:intergenic
TCACCTTAAGAAGATACATC+TGG 0.406097 8.4:+42621508 None:intergenic
TTGAATCCAAAGAATATAAG+TGG 0.422305 8.4:+42622504 None:intergenic
ATTGATGGATTTCAACCTCT+TGG 0.423461 8.4:+42622376 None:intergenic
ATATCCTTCTATGTGAATAT+AGG 0.424387 8.4:+42621705 None:intergenic
CTGACCTATATTCACATAGA+AGG 0.433553 8.4:-42621709 MS.gene63260:CDS
ACATCGTTGGTGTGGAAGAC+CGG 0.442773 8.4:+42623759 None:intergenic
CGATCTAATTTGCGAAACTC+TGG 0.442892 8.4:+42622013 None:intergenic
ATGGATCCCCGTGTCACATT+GGG 0.443233 8.4:-42622317 MS.gene63260:CDS
CTCTATACGGGATGTCATCT+CGG 0.461434 8.4:+42622201 None:intergenic
GGATTTCAACCTCTTGGAGC+TGG 0.461935 8.4:+42622382 None:intergenic
CGACAAATTCACTCTCGCTC+GGG 0.466346 8.4:+42622243 None:intergenic
CAGAAGATTTGAGTTAAAGC+AGG 0.472414 8.4:+42621973 None:intergenic
CCTGAAGTTGCTCCCAAAGA+TGG 0.477012 8.4:+42623805 None:intergenic
ATTTCCAAAGTTTCCAGCAC+AGG 0.477786 8.4:+42622458 None:intergenic
CATCTGGGTCAAATTTAAGT+TGG 0.478361 8.4:+42621524 None:intergenic
TTTAGAACAGGGGAATGAAA+TGG 0.479276 8.4:+42622040 None:intergenic
CCATCAATGACAATTTCACT+TGG 0.483121 8.4:-42622361 MS.gene63260:CDS
AAGATTAACAAATGTATCAA+AGG 0.488317 8.4:-42621560 MS.gene63260:CDS
CGTGTCACATTGGGCATCGT+TGG 0.489651 8.4:-42622308 MS.gene63260:CDS
TTATGAGAACTTGAAGCATA+TGG 0.489867 8.4:+42621930 None:intergenic
TCTGGCTGCCATGTTCTCAA+TGG 0.500024 8.4:+42621766 None:intergenic
CTTCTATGTGAATATAGGTC+AGG 0.500493 8.4:+42621710 None:intergenic
AACGATGCCCAATGTGACAC+GGG 0.503703 8.4:+42622310 None:intergenic
CATCGTTGGTGTGGAAGACC+GGG 0.505127 8.4:+42623760 None:intergenic
CTTTAAACCTCTTCTCATCT+GGG 0.510564 8.4:+42623640 None:intergenic
CGAGATGACATCCCGTATAG+AGG 0.513159 8.4:-42622200 MS.gene63260:CDS
CAGTTGACGAAGAGGGAAGA+AGG 0.515953 8.4:+42623537 None:intergenic
ATTCACATAGAAGGATATCA+AGG 0.519668 8.4:-42621700 MS.gene63260:CDS
AACCTCTTGGAGCTGGAGGA+AGG 0.523174 8.4:+42622389 None:intergenic
CACCTTAAGAAGATACATCT+GGG 0.528482 8.4:+42621509 None:intergenic
TCGACAAATTCACTCTCGCT+CGG 0.533557 8.4:+42622242 None:intergenic
TATGGATCCCCGTGTCACAT+TGG 0.538550 8.4:-42622318 MS.gene63260:CDS
GGCTGCCATGTTCTCAATGG+TGG 0.539440 8.4:+42621769 None:intergenic
TCAGAAACTCTTGTTGACTC+TGG 0.541237 8.4:+42621748 None:intergenic
GACAAATTCACTCTCGCTCG+GGG 0.542706 8.4:+42622244 None:intergenic
AGATTAACAAATGTATCAAA+GGG 0.543019 8.4:-42621559 MS.gene63260:CDS
TGCAGTCTTAAGGGTGCTAT+CGG 0.543329 8.4:-42622581 MS.gene63260:intron
GTCAATACCCAGATGAGAAG+AGG 0.546247 8.4:-42623647 MS.gene63260:CDS
CAACTTCAAAGACTCGGACC+CGG 0.551274 8.4:-42623778 MS.gene63260:CDS
AACAACCACCATTGAGAACA+TGG 0.552420 8.4:-42621774 MS.gene63260:CDS
TAAGGGTGCTATCGGAATGA+AGG 0.555319 8.4:-42622573 MS.gene63260:CDS
GATTTGATGCACCTCTATAC+GGG 0.555738 8.4:+42622189 None:intergenic
TTTCAACCTCTTGGAGCTGG+AGG 0.556210 8.4:+42622385 None:intergenic
CAACGATGCCCAATGTGACA+CGG 0.557275 8.4:+42622309 None:intergenic
CACCTTCCTCCAGCTCCAAG+AGG 0.560067 8.4:-42622391 MS.gene63260:CDS
GTTGTTGCAGTTGACGAAGA+GGG 0.580530 8.4:+42623530 None:intergenic
CTATGTGAATATAGGTCAGG+TGG 0.580589 8.4:+42621713 None:intergenic
AGAAGATTTGAGTTAAAGCA+GGG 0.583843 8.4:+42621974 None:intergenic
TTTCCAAAGTTTCCAGCACA+GGG 0.585440 8.4:+42622459 None:intergenic
TTTGAATGTTGCGGGATCTG+CGG 0.592777 8.4:+42623685 None:intergenic
GTGTGGATCCGTGCTGCCGT+TGG 0.593830 8.4:-42623618 MS.gene63260:CDS
TCATGAGTCTCGTCTCTCGA+AGG 0.598281 8.4:-42623833 MS.gene63260:CDS
ATGAGAAGAGGTTTAAAGTG+TGG 0.614314 8.4:-42623635 MS.gene63260:CDS
TGAGTCTCGTCTCTCGAAGG+TGG 0.619102 8.4:-42623830 MS.gene63260:CDS
CTCTTGGAGCTGGAGGAAGG+TGG 0.621502 8.4:+42622392 None:intergenic
GGTTGTTGCAGTTGACGAAG+AGG 0.624738 8.4:+42623529 None:intergenic
CTGGGTATTGACAACTGAGA+CGG 0.625065 8.4:+42623658 None:intergenic
TCTCATAATCCAAAGCAGCA+AGG 0.625493 8.4:-42621915 MS.gene63260:intron
AGATATGTCTTCTTCCACAA+GGG 0.632627 8.4:+42622433 None:intergenic
AAGATATGTCTTCTTCCACA+AGG 0.632852 8.4:+42622432 None:intergenic
TGGCATCTTTCATAGTATGG+TGG 0.632883 8.4:-42622288 MS.gene63260:CDS
GGTGGGGTCCAACGGCAGCA+CGG 0.634582 8.4:+42623610 None:intergenic
AACTTTGGAAATTACCCTTG+TGG 0.641345 8.4:-42622447 MS.gene63260:CDS
TATGTGAATATAGGTCAGGT+GGG 0.652099 8.4:+42621714 None:intergenic
TAACTTACGTGAGGGAAACG+AGG 0.652444 8.4:+42623508 None:intergenic
CTCTGGATAGTTTAGAACAG+GGG 0.654545 8.4:+42622030 None:intergenic
ACTCTGGATAGTTTAGAACA+GGG 0.672908 8.4:+42622029 None:intergenic
ACGATGCCCAATGTGACACG+GGG 0.730418 8.4:+42622311 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTAAATGTCATTTAT+AGG + chr8.4:42622322-42622341 None:intergenic 10.0%
!!! AAAATTAAATGTCATTTATA+GGG + chr8.4:42622321-42622340 None:intergenic 10.0%
!!! ATACCTATATTTTTATTATA+AGG - chr8.4:42622573-42622592 MS.gene63260:CDS 10.0%
!!! ATTAAGAAAAAAATAGATTT+TGG + chr8.4:42622139-42622158 None:intergenic 10.0%
!! CTACCTTATAATAAAAATAT+AGG + chr8.4:42622579-42622598 None:intergenic 15.0%
!!! AGTTTAATTTAATCAATCTT+TGG - chr8.4:42623467-42623486 MS.gene63260:intron 15.0%
!!! ATTTTCAATTAAGATTTCAA+GGG - chr8.4:42622342-42622361 MS.gene63260:CDS 15.0%
!!! TATTTTCAATTAAGATTTCA+AGG - chr8.4:42622341-42622360 MS.gene63260:CDS 15.0%
!! AAAAAAAATCCAAAACAAAC+AGG + chr8.4:42621981-42622000 None:intergenic 20.0%
!! AAAATAAATTAACTTACGTG+AGG + chr8.4:42621848-42621867 None:intergenic 20.0%
!! AAATAAATTAACTTACGTGA+GGG + chr8.4:42621847-42621866 None:intergenic 20.0%
!! AAGATTAACAAATGTATCAA+AGG - chr8.4:42623784-42623803 MS.gene63260:CDS 20.0%
!! AGATTAACAAATGTATCAAA+GGG - chr8.4:42623785-42623804 MS.gene63260:CDS 20.0%
!! AGGTAGTTCAAAAAAATAAT+CGG + chr8.4:42621790-42621809 None:intergenic 20.0%
!! TTTAACTAAAAAGAGTTGTT+AGG + chr8.4:42622490-42622509 None:intergenic 20.0%
!!! AAGGTGAAGTTTTATTTTTA+TGG - chr8.4:42623201-42623220 MS.gene63260:intron 20.0%
!!! ATATAGGTATGTATGTTTTA+AGG + chr8.4:42622563-42622582 None:intergenic 20.0%
!!! TCTATTTTTTTCTTAATGCA+AGG - chr8.4:42622142-42622161 MS.gene63260:intron 20.0%
!!! TGAAATAGATTTTCATTTTG+AGG + chr8.4:42622106-42622125 None:intergenic 20.0%
!!! TGTTTGTTTTGGATTTTTTT+TGG - chr8.4:42621980-42621999 MS.gene63260:CDS 20.0%
!!! TGTTTTTGTTGATATTTCAA+TGG - chr8.4:42623733-42623752 MS.gene63260:CDS 20.0%
!!! TTGTTAATCTTTTGATAGAA+CGG + chr8.4:42623775-42623794 None:intergenic 20.0%
! ACAAATTTCAAACATAAGTC+AGG + chr8.4:42622608-42622627 None:intergenic 25.0%
! ATATCCTTCTATGTGAATAT+AGG + chr8.4:42623642-42623661 None:intergenic 25.0%
! CAAAATCCACTTATATTCTT+TGG - chr8.4:42622834-42622853 MS.gene63260:intron 25.0%
! GAGAAGAATACATTAAATGA+TGG + chr8.4:42622673-42622692 None:intergenic 25.0%
! GGTAGTTCAAAAAAATAATC+GGG + chr8.4:42621789-42621808 None:intergenic 25.0%
! TACACAATAAAAACAAGTGA+TGG + chr8.4:42623274-42623293 None:intergenic 25.0%
! TTGAATCCAAAGAATATAAG+TGG + chr8.4:42622843-42622862 None:intergenic 25.0%
! TTTGCATCATTCAACATTAA+AGG - chr8.4:42623182-42623201 MS.gene63260:intron 25.0%
!! CTTATTATACTAGCTTATAG+CGG + chr8.4:42622439-42622458 None:intergenic 25.0%
!! TTACTTTGAGTTTGATGATA+TGG - chr8.4:42623008-42623027 MS.gene63260:intron 25.0%
!!! GTATGTATGTTTTAAGGATT+TGG + chr8.4:42622557-42622576 None:intergenic 25.0%
ATTCACATAGAAGGATATCA+AGG - chr8.4:42623644-42623663 MS.gene63260:CDS 30.0%
GTAAAAAATAGAGCTTACAG+TGG - chr8.4:42621924-42621943 MS.gene63260:CDS 30.0%
TTATGAGAACTTGAAGCATA+TGG + chr8.4:42623417-42623436 None:intergenic 30.0%
! AGAAGATTTGAGTTAAAGCA+GGG + chr8.4:42623373-42623392 None:intergenic 30.0%
! AGCTTTTAACCGATAGATTA+TGG - chr8.4:42621954-42621973 MS.gene63260:CDS 30.0%
! GCTGAATATATTTTGCGAAA+CGG - chr8.4:42623689-42623708 MS.gene63260:CDS 30.0%
! GCTTTTAACCGATAGATTAT+GGG - chr8.4:42621955-42621974 MS.gene63260:CDS 30.0%
! TACATTAAATGATGGAAGTG+AGG + chr8.4:42622665-42622684 None:intergenic 30.0%
!! ATAAGCTTGTTAGAAAGTGA+CGG - chr8.4:42622461-42622480 MS.gene63260:CDS 30.0%
!! GAACTTTTTGATAACATCGT+TGG + chr8.4:42621601-42621620 None:intergenic 30.0%
AAGATATGTCTTCTTCCACA+AGG + chr8.4:42622915-42622934 None:intergenic 35.0%
ACTTTAAACCTCTTCTCATC+TGG + chr8.4:42621708-42621727 None:intergenic 35.0%
AGATATGTCTTCTTCCACAA+GGG + chr8.4:42622914-42622933 None:intergenic 35.0%
AGATTTGATGCACCTCTATA+CGG + chr8.4:42623159-42623178 None:intergenic 35.0%
CATCTGGGTCAAATTTAAGT+TGG + chr8.4:42623823-42623842 None:intergenic 35.0%
CCAAGTGAAATTGTCATTGA+TGG + chr8.4:42622986-42623005 None:intergenic 35.0%
CCATCAATGACAATTTCACT+TGG - chr8.4:42622983-42623002 MS.gene63260:intron 35.0%
CCTTCAAAAACTCTCTCTTA+AGG - chr8.4:42621755-42621774 MS.gene63260:CDS 35.0%
CTGACCTATATTCACATAGA+AGG - chr8.4:42623635-42623654 MS.gene63260:CDS 35.0%
CTTCTATGTGAATATAGGTC+AGG + chr8.4:42623637-42623656 None:intergenic 35.0%
CTTTAAACCTCTTCTCATCT+GGG + chr8.4:42621707-42621726 None:intergenic 35.0%
TATGTGAATATAGGTCAGGT+GGG + chr8.4:42623633-42623652 None:intergenic 35.0%
TGAAAACACTGCAGTCTTAA+GGG - chr8.4:42622754-42622773 MS.gene63260:intron 35.0%
TTGAAAACACTGCAGTCTTA+AGG - chr8.4:42622753-42622772 MS.gene63260:intron 35.0%
! AACTCTGGATAGTTTAGAAC+AGG + chr8.4:42623319-42623338 None:intergenic 35.0%
! AACTTTGGAAATTACCCTTG+TGG - chr8.4:42622897-42622916 MS.gene63260:intron 35.0%
! ACTCTGGATAGTTTAGAACA+GGG + chr8.4:42623318-42623337 None:intergenic 35.0%
! ATGAGAAGAGGTTTAAAGTG+TGG - chr8.4:42621709-42621728 MS.gene63260:CDS 35.0%
! ATTTTGAGCTGCTTCAATGA+TGG + chr8.4:42622820-42622839 None:intergenic 35.0%
! CAGAAGATTTGAGTTAAAGC+AGG + chr8.4:42623374-42623393 None:intergenic 35.0%
! CCTTAAGAGAGAGTTTTTGA+AGG + chr8.4:42621758-42621777 None:intergenic 35.0%
! GGTTTTCAACTTCAAAGACT+CGG - chr8.4:42621560-42621579 MS.gene63260:CDS 35.0%
! TTAAATGATGGAAGTGAGGT+TGG + chr8.4:42622661-42622680 None:intergenic 35.0%
! TTTAGAACAGGGGAATGAAA+TGG + chr8.4:42623307-42623326 None:intergenic 35.0%
! TTTTCAAAAGACAGACACAG+AGG + chr8.4:42622740-42622759 None:intergenic 35.0%
!! AAGAGAGAGTTTTTGAAGGT+GGG + chr8.4:42621754-42621773 None:intergenic 35.0%
!! ATTGATGGATTTCAACCTCT+TGG + chr8.4:42622971-42622990 None:intergenic 35.0%
!! TAAGAGAGAGTTTTTGAAGG+TGG + chr8.4:42621755-42621774 None:intergenic 35.0%
!! TTTTGATAACATCGTTGGTG+TGG + chr8.4:42621596-42621615 None:intergenic 35.0%
!!! TTTTTTACCAAACCCAGACA+TGG + chr8.4:42621912-42621931 None:intergenic 35.0%
AACAACCACCATTGAGAACA+TGG - chr8.4:42623570-42623589 MS.gene63260:CDS 40.0%
ATTTCCAAAGTTTCCAGCAC+AGG + chr8.4:42622889-42622908 None:intergenic 40.0%
CAAACAGGCCCATAATCTAT+CGG + chr8.4:42621966-42621985 None:intergenic 40.0%
CGTTGGCATCTTTCATAGTA+TGG - chr8.4:42623053-42623072 MS.gene63260:intron 40.0%
CTATGTGAATATAGGTCAGG+TGG + chr8.4:42623634-42623653 None:intergenic 40.0%
GATTTGATGCACCTCTATAC+GGG + chr8.4:42623158-42623177 None:intergenic 40.0%
TCAAAAGTTGCCTTTCTCTC+TGG - chr8.4:42622859-42622878 MS.gene63260:intron 40.0%
TCTCATAATCCAAAGCAGCA+AGG - chr8.4:42623429-42623448 MS.gene63260:intron 40.0%
TGGCATCTTTCATAGTATGG+TGG - chr8.4:42623056-42623075 MS.gene63260:intron 40.0%
TTTCCAAAGTTTCCAGCACA+GGG + chr8.4:42622888-42622907 None:intergenic 40.0%
! ACGGAACTTTTGAATGTTGC+GGG + chr8.4:42621670-42621689 None:intergenic 40.0%
! CACTTGTTACTACCATGTCT+GGG - chr8.4:42621897-42621916 MS.gene63260:intron 40.0%
! CGATCTAATTTGCGAAACTC+TGG + chr8.4:42623334-42623353 None:intergenic 40.0%
! CTCTGGATAGTTTAGAACAG+GGG + chr8.4:42623317-42623336 None:intergenic 40.0%
! GACGGAACTTTTGAATGTTG+CGG + chr8.4:42621671-42621690 None:intergenic 40.0%
! GATTATGGGCCTGTTTGTTT+TGG - chr8.4:42621969-42621988 MS.gene63260:CDS 40.0%
!! AGAGAGAGTTTTTGAAGGTG+GGG + chr8.4:42621753-42621772 None:intergenic 40.0%
!! TCAGAAACTCTTGTTGACTC+TGG + chr8.4:42623599-42623618 None:intergenic 40.0%
!! TTTGCGAAACGGACTTGTTT+TGG - chr8.4:42623700-42623719 MS.gene63260:CDS 40.0%
CCAGACATGGTAGTAACAAG+TGG + chr8.4:42621899-42621918 None:intergenic 45.0%
CTCTATACGGGATGTCATCT+CGG + chr8.4:42623146-42623165 None:intergenic 45.0%
GTCAATACCCAGATGAGAAG+AGG - chr8.4:42621697-42621716 MS.gene63260:CDS 45.0%
GTTACTACCATGTCTGGGTT+TGG - chr8.4:42621902-42621921 MS.gene63260:intron 45.0%
GTTCCTCCTGCCAACAATAA+GGG + chr8.4:42623553-42623572 None:intergenic 45.0%
GTTGTTGCAGTTGACGAAGA+GGG + chr8.4:42621817-42621836 None:intergenic 45.0%
TAACTTACGTGAGGGAAACG+AGG + chr8.4:42621839-42621858 None:intergenic 45.0%
TCGACAAATTCACTCTCGCT+CGG + chr8.4:42623105-42623124 None:intergenic 45.0%
TCTATCCGCACATTGAGTCA+TGG - chr8.4:42623519-42623538 MS.gene63260:CDS 45.0%
TGTTCCTCCTGCCAACAATA+AGG + chr8.4:42623554-42623573 None:intergenic 45.0%
TTTGAATGTTGCGGGATCTG+CGG + chr8.4:42621662-42621681 None:intergenic 45.0%
! CCACTTGTTACTACCATGTC+TGG - chr8.4:42621896-42621915 MS.gene63260:intron 45.0%
! GTCAACAAACCTTGCTGCTT+TGG + chr8.4:42623441-42623460 None:intergenic 45.0%
!! CTGGGTATTGACAACTGAGA+CGG + chr8.4:42621689-42621708 None:intergenic 45.0%
!! TAAGGGTGCTATCGGAATGA+AGG - chr8.4:42622771-42622790 MS.gene63260:intron 45.0%
!! TGCAGTCTTAAGGGTGCTAT+CGG - chr8.4:42622763-42622782 MS.gene63260:intron 45.0%
!! TTTTTGAAGGTGGGGTCCAA+CGG + chr8.4:42621745-42621764 None:intergenic 45.0%
!!! AAATTTGTTTATTTTTTTTT+TGG - chr8.4:42622620-42622639 MS.gene63260:intron 5.0%
AACGATGCCCAATGTGACAC+GGG + chr8.4:42623037-42623056 None:intergenic 50.0%
ACATCGTTGGTGTGGAAGAC+CGG + chr8.4:42621588-42621607 None:intergenic 50.0%
ATGGATCCCCGTGTCACATT+GGG - chr8.4:42623027-42623046 MS.gene63260:intron 50.0%
CAACGATGCCCAATGTGACA+CGG + chr8.4:42623038-42623057 None:intergenic 50.0%
CAACTTCAAAGACTCGGACC+CGG - chr8.4:42621566-42621585 MS.gene63260:CDS 50.0%
CAGTTGACGAAGAGGGAAGA+AGG + chr8.4:42621810-42621829 None:intergenic 50.0%
CCATGCCATGACTCAATGTG+CGG + chr8.4:42623527-42623546 None:intergenic 50.0%
CCTGAAGTTGCTCCCAAAGA+TGG + chr8.4:42621542-42621561 None:intergenic 50.0%
CGACAAATTCACTCTCGCTC+GGG + chr8.4:42623104-42623123 None:intergenic 50.0%
CGAGATGACATCCCGTATAG+AGG - chr8.4:42623144-42623163 MS.gene63260:intron 50.0%
CTCGGATGCAGTCGAGAATA+GGG + chr8.4:42623128-42623147 None:intergenic 50.0%
GACAAATTCACTCTCGCTCG+GGG + chr8.4:42623103-42623122 None:intergenic 50.0%
GGATTTCAACCTCTTGGAGC+TGG + chr8.4:42622965-42622984 None:intergenic 50.0%
GGCATGGTGTCCCTTATTGT+TGG - chr8.4:42623540-42623559 MS.gene63260:CDS 50.0%
GGTTGTTGCAGTTGACGAAG+AGG + chr8.4:42621818-42621837 None:intergenic 50.0%
TATGGATCCCCGTGTCACAT+TGG - chr8.4:42623026-42623045 MS.gene63260:intron 50.0%
TCGAAGGTGGCACCATCTTT+GGG - chr8.4:42621527-42621546 MS.gene63260:CDS 50.0%
TCTCGGATGCAGTCGAGAAT+AGG + chr8.4:42623129-42623148 None:intergenic 50.0%
TCTGGCTGCCATGTTCTCAA+TGG + chr8.4:42623581-42623600 None:intergenic 50.0%
TGGTGTCCCTTATTGTTGGC+AGG - chr8.4:42623544-42623563 MS.gene63260:CDS 50.0%
TGTCCCTTATTGTTGGCAGG+AGG - chr8.4:42623547-42623566 MS.gene63260:CDS 50.0%
TTTCAACCTCTTGGAGCTGG+AGG + chr8.4:42622962-42622981 None:intergenic 50.0%
! CCATCTTTGGGAGCAACTTC+AGG - chr8.4:42621539-42621558 MS.gene63260:CDS 50.0%
!!! GCGAAACGGACTTGTTTTGG+AGG - chr8.4:42623703-42623722 MS.gene63260:CDS 50.0%
AACCTCTTGGAGCTGGAGGA+AGG + chr8.4:42622958-42622977 None:intergenic 55.0%
ACGATGCCCAATGTGACACG+GGG + chr8.4:42623036-42623055 None:intergenic 55.0%
CATCGTTGGTGTGGAAGACC+GGG + chr8.4:42621587-42621606 None:intergenic 55.0%
CCGCACATTGAGTCATGGCA+TGG - chr8.4:42623524-42623543 MS.gene63260:CDS 55.0%
CTCGAAGGTGGCACCATCTT+TGG - chr8.4:42621526-42621545 MS.gene63260:CDS 55.0%
CTGCCCTGTGCTGGAAACTT+TGG - chr8.4:42622882-42622901 MS.gene63260:intron 55.0%
GGCTGCCATGTTCTCAATGG+TGG + chr8.4:42623578-42623597 None:intergenic 55.0%
TGAGTCTCGTCTCTCGAAGG+TGG - chr8.4:42621514-42621533 MS.gene63260:CDS 55.0%
! CGTGTCACATTGGGCATCGT+TGG - chr8.4:42623036-42623055 MS.gene63260:intron 55.0%
CACAGGGCAGCCAGAGAGAA+AGG + chr8.4:42622872-42622891 None:intergenic 60.0%
CACCTTCCTCCAGCTCCAAG+AGG - chr8.4:42622953-42622972 MS.gene63260:intron 60.0%
CTCTTGGAGCTGGAGGAAGG+TGG + chr8.4:42622955-42622974 None:intergenic 60.0%
GTGTGGATCCGTGCTGCCGT+TGG - chr8.4:42621726-42621745 MS.gene63260:CDS 65.0%
TCTCTCTGGCTGCCCTGTGC+TGG - chr8.4:42622873-42622892 MS.gene63260:intron 65.0%
GGTGGGGTCCAACGGCAGCA+CGG + chr8.4:42621737-42621756 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 42621513 42623853 42621513 ID=MS.gene63260
chr8.4 mRNA 42621513 42623853 42621513 ID=MS.gene63260.t1;Parent=MS.gene63260
chr8.4 exon 42623516 42623853 42623516 ID=MS.gene63260.t1.exon1;Parent=MS.gene63260.t1
chr8.4 CDS 42623516 42623853 42623516 ID=cds.MS.gene63260.t1;Parent=MS.gene63260.t1
chr8.4 exon 42622163 42622598 42622163 ID=MS.gene63260.t1.exon2;Parent=MS.gene63260.t1
chr8.4 CDS 42622163 42622598 42622163 ID=cds.MS.gene63260.t1;Parent=MS.gene63260.t1
chr8.4 exon 42621916 42622065 42621916 ID=MS.gene63260.t1.exon3;Parent=MS.gene63260.t1
chr8.4 CDS 42621916 42622065 42621916 ID=cds.MS.gene63260.t1;Parent=MS.gene63260.t1
chr8.4 exon 42621513 42621800 42621513 ID=MS.gene63260.t1.exon4;Parent=MS.gene63260.t1
chr8.4 CDS 42621513 42621800 42621513 ID=cds.MS.gene63260.t1;Parent=MS.gene63260.t1
Gene Sequence

>MS.gene63260

ATGAGTCTCGTCTCTCGAAGGTGGCACCATCTTTGGGAGCAACTTCAGGTTTTCAACTTCAAAGACTCGGACCCGGTCTTCCACACCAACGATGTTATCAAAAAGTTCAACATGTTTGCTTATTTCGTTGACGCTGTGCTCGCTCTCCGCAGATCCCGCAACATTCAAAAGTTCCGTCTCAGTTGTCAATACCCAGATGAGAAGAGGTTTAAAGTGTGGATCCGTGCTGCCGTTGGACCCCACCTTCAAAAACTCTCTCTTAAGGTTTTAAATCCCGATTATTTTTTTGAACTACCTTCTTCCCTCTTCGTCAACTGCAACAACCTCGTTTCCCTCACGTAAGTTAATTTATTTTAAAGCTATTATCACAATATCAACACACACCACTTGTTACTACCATGTCTGGGTTTGGTAAAAAATAGAGCTTACAGTGGAAAAACTAGCTTTTAACCGATAGATTATGGGCCTGTTTGTTTTGGATTTTTTTTGGAAAAAATCTATTTTTTTGAGTAAAAAATCACTTTTTTAGTTTTTGTTGTGTTTGTTTGAGATTTTTAAAAAATCTATTTTGCTAAAAAAATCATTTTTAGCCTCAAAATGAAAATCTATTTCAAGTAGCTTTTCCAAAATCTATTTTTTTCTTAATGCAAGGAACTCAAGTGTAAGTGTTTCAAATCTCAAAAAAATGATTTTTATGATATTAGACAAACAGAAATAGTTTTAGATTTTTTTAAAAATCTCTAAAAAAAAATCTCTAGATTCTTTTAACTCAAAATCTATTTTTTTTAAAGCTGAAACAAACGCACCCTATAAATGACATTTAATTTTTATTTTCAATTAAGATTTCAAGGGTAAAATTGAGATAAATTAATGATAAGCTATAATCTTTAATCTATAAGCTACTTGAGTTAGCATATCAAAAACCGCTATAAGCTAGTATAATAAGCTATAAGCTTGTTAGAAAGTGACGGTTACCTAACAACTCTTTTTAGTTAAATGAACTTATTAAACATAAGCTATAAGCTATAAAATCATGTCTTACCAAATCCTTAAAACATACATACCTATATTTTTATTATAAGGTAGAGTTAGCCTGACTTATGTTTGAAATTTGTTTATTTTTTTTTTGGTTTTACATATTATGTCCAACCTCACTTCCATCATTTAATGTATTCTTCTCTTTCTATTTCTAACAAGTAAACAAATATACAATGTTATCTATAACCTCTGTGTCTGTCTTTTGAAAACACTGCAGTCTTAAGGGTGCTATCGGAATGAAGGTTGAACATTCTTCAGTTCATTTTCCATCATTGAAGCAGCTCAAAATCCACTTATATTCTTTGGATTCAAAAGTTGCCTTTCTCTCTGGCTGCCCTGTGCTGGAAACTTTGGAAATTACCCTTGTGGAAGAAGACATATCTTTGACTAAGCTTAATCTTCCACCTTCCTCCAGCTCCAAGAGGTTGAAATCCATCAATGACAATTTCACTTGGACTTACTTTGAGTTTGATGATATGGATCCCCGTGTCACATTGGGCATCGTTGGCATCTTTCATAGTATGGTGGAAGCATTTCTTGATGTCTTTTCCCCGAGCGAGAGTGAATTTGTCGACCCTATTCTCGACTGCATCCGAGATGACATCCCGTATAGAGGTGCATCAAATCTACTTTTGCATCATTCAACATTAAAGGTGAAGTTTTATTTTTATGGTTAATATGTTTTTATATATGTAAATTGTAATTGACACTAATATTGCGCCATCACTTGTTTTTATTGTGTACAATCAGTTGCCATTTCATTCCCCTGTTCTAAACTATCCAGAGTTTCGCAAATTAGATCGTCTAAAGTTCATTCTTCCCTGCTTTAACTCAAATCTTCTGCTCAACGTGCTTGATAAATGCCATATGCTTCAAGTTCTCATAATCCAAAGCAGCAAGGTTTGTTGACTTCTCAAGTTTAATTTAATCAATCTTTGGTCTGTCTATATAATATGCATGCATATGTATCTATCCGCACATTGAGTCATGGCATGGTGTCCCTTATTGTTGGCAGGAGGAACAACCACCATTGAGAACATGGCAGCCAGAGTCAACAAGAGTTTCTGAATGTCTCAAGTCCCACCTGACCTATATTCACATAGAAGGATATCAAGGACTTGAAGATGAGCTGACATTTGCTGAATATATTTTGCGAAACGGACTTGTTTTGGAGGCAATGCTTGTTTTTGTTGATATTTCAATGGACATAACGAACAAGTACCGTTCTATCAAAAGATTAACAAATGTATCAAAGGGCTCTGTCAAATGCCAACTTAAATTTGACCCAGATGTATCTTCTTAA
Protein sequence

>MS.gene63260.t1

MSLVSRRWHHLWEQLQVFNFKDSDPVFHTNDVIKKFNMFAYFVDAVLALRRSRNIQKFRLSCQYPDEKRFKVWIRAAVGPHLQKLSLKVLNPDYFFELPSSLFVNCNNLVSLTLKGAIGMKVEHSSVHFPSLKQLKIHLYSLDSKVAFLSGCPVLETLEITLVEEDISLTKLNLPPSSSSKRLKSINDNFTWTYFEFDDMDPRVTLGIVGIFHSMVEAFLDVFSPSESEFVDPILDCIRDDIPYRGASNLLLHHSTLKLPFHSPVLNYPEFRKLDRLKFILPCFNSNLLLNVLDKCHMLQVLIIQSSKEEQPPLRTWQPESTRVSECLKSHLTYIHIEGYQGLEDELTFAEYILRNGLVLEAMLVFVDISMDITNKYRSIKRLTNVSKGSVKCQLKFDPDVSS