Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28290.t1 | XP_024631453.1 | 94.4 | 213 | 12 | 0 | 1 | 213 | 1 | 213 | 3.20E-109 | 404.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28290.t1 | A0A396J3H9 | 94.4 | 213 | 12 | 0 | 1 | 213 | 1 | 213 | 2.3e-109 | 404.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene28290 | -0.818708 | 1.70E-52 | -1.69E-46 |
| MS.gene049171 | MS.gene28290 | -0.849029 | 4.20E-60 | -1.69E-46 |
| MS.gene049217 | MS.gene28290 | -0.86599 | 3.95E-65 | -1.69E-46 |
| MS.gene049222 | MS.gene28290 | -0.867779 | 1.06E-65 | -1.69E-46 |
| MS.gene049232 | MS.gene28290 | -0.820693 | 5.99E-53 | -1.69E-46 |
| MS.gene049285 | MS.gene28290 | -0.837983 | 3.77E-57 | -1.69E-46 |
| MS.gene049320 | MS.gene28290 | -0.837906 | 3.94E-57 | -1.69E-46 |
| MS.gene049549 | MS.gene28290 | 0.800848 | 1.19E-48 | -1.69E-46 |
| MS.gene049609 | MS.gene28290 | -0.805721 | 1.16E-49 | -1.69E-46 |
| MS.gene049611 | MS.gene28290 | 0.824579 | 7.48E-54 | -1.69E-46 |
| MS.gene049627 | MS.gene28290 | -0.806068 | 9.84E-50 | -1.69E-46 |
| MS.gene049633 | MS.gene28290 | -0.83288 | 7.34E-56 | -1.69E-46 |
| MS.gene049634 | MS.gene28290 | 0.832785 | 7.75E-56 | -1.69E-46 |
| MS.gene05010 | MS.gene28290 | -0.815801 | 7.67E-52 | -1.69E-46 |
| MS.gene050163 | MS.gene28290 | -0.82217 | 2.73E-53 | -1.69E-46 |
| MS.gene050165 | MS.gene28290 | -0.823271 | 1.52E-53 | -1.69E-46 |
| MS.gene050170 | MS.gene28290 | -0.826469 | 2.67E-54 | -1.69E-46 |
| MS.gene050216 | MS.gene28290 | -0.815824 | 7.58E-52 | -1.69E-46 |
| MS.gene050323 | MS.gene28290 | -0.840792 | 7.03E-58 | -1.69E-46 |
| MS.gene050333 | MS.gene28290 | -0.827056 | 1.93E-54 | -1.69E-46 |
| MS.gene050380 | MS.gene28290 | 0.801579 | 8.42E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene28290 | 0.85292 | 3.36E-61 | -1.69E-46 |
| MS.gene050444 | MS.gene28290 | -0.825166 | 5.44E-54 | -1.69E-46 |
| MS.gene050496 | MS.gene28290 | -0.816748 | 4.71E-52 | -1.69E-46 |
| MS.gene050504 | MS.gene28290 | -0.855193 | 7.43E-62 | -1.69E-46 |
| MS.gene050518 | MS.gene28290 | -0.824716 | 6.94E-54 | -1.69E-46 |
| MS.gene050577 | MS.gene28290 | -0.835711 | 1.43E-56 | -1.69E-46 |
| MS.gene050612 | MS.gene28290 | -0.848101 | 7.59E-60 | -1.69E-46 |
| MS.gene050647 | MS.gene28290 | 0.807091 | 5.99E-50 | -1.69E-46 |
| MS.gene050675 | MS.gene28290 | -0.821772 | 3.38E-53 | -1.69E-46 |
| MS.gene050699 | MS.gene28290 | -0.832 | 1.21E-55 | -1.69E-46 |
| MS.gene050747 | MS.gene28290 | -0.824746 | 6.83E-54 | -1.69E-46 |
| MS.gene050772 | MS.gene28290 | -0.826432 | 2.72E-54 | -1.69E-46 |
| MS.gene05080 | MS.gene28290 | -0.831524 | 1.59E-55 | -1.69E-46 |
| MS.gene050868 | MS.gene28290 | -0.865517 | 5.58E-65 | -1.69E-46 |
| MS.gene050871 | MS.gene28290 | -0.864316 | 1.33E-64 | -1.69E-46 |
| MS.gene050883 | MS.gene28290 | 0.823406 | 1.41E-53 | -1.69E-46 |
| MS.gene050904 | MS.gene28290 | -0.816645 | 4.97E-52 | -1.69E-46 |
| MS.gene050950 | MS.gene28290 | -0.813601 | 2.36E-51 | -1.69E-46 |
| MS.gene050986 | MS.gene28290 | -0.817851 | 2.66E-52 | -1.69E-46 |
| MS.gene05104 | MS.gene28290 | 0.817899 | 2.60E-52 | -1.69E-46 |
| MS.gene051127 | MS.gene28290 | 0.808492 | 3.02E-50 | -1.69E-46 |
| MS.gene051138 | MS.gene28290 | 0.83851 | 2.75E-57 | -1.69E-46 |
| MS.gene051167 | MS.gene28290 | 0.843627 | 1.25E-58 | -1.69E-46 |
| MS.gene051317 | MS.gene28290 | -0.810878 | 9.26E-51 | -1.69E-46 |
| MS.gene051364 | MS.gene28290 | 0.821238 | 4.49E-53 | -1.69E-46 |
| MS.gene051366 | MS.gene28290 | 0.817441 | 3.29E-52 | -1.69E-46 |
| MS.gene05141 | MS.gene28290 | 0.826553 | 2.55E-54 | -1.69E-46 |
| MS.gene051440 | MS.gene28290 | 0.816056 | 6.73E-52 | -1.69E-46 |
| MS.gene051651 | MS.gene28290 | 0.800076 | 1.71E-48 | -1.69E-46 |
| MS.gene051666 | MS.gene28290 | -0.812605 | 3.90E-51 | -1.69E-46 |
| MS.gene051694 | MS.gene28290 | -0.831857 | 1.32E-55 | -1.69E-46 |
| MS.gene051858 | MS.gene28290 | -0.848201 | 7.13E-60 | -1.69E-46 |
| MS.gene051946 | MS.gene28290 | 0.805294 | 1.43E-49 | -1.69E-46 |
| MS.gene052015 | MS.gene28290 | 0.803534 | 3.33E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene28290 | -0.839847 | 1.24E-57 | -1.69E-46 |
| MS.gene052211 | MS.gene28290 | 0.809095 | 2.24E-50 | -1.69E-46 |
| MS.gene05234 | MS.gene28290 | 0.811932 | 5.47E-51 | -1.69E-46 |
| MS.gene052482 | MS.gene28290 | -0.853092 | 3.00E-61 | -1.69E-46 |
| MS.gene052486 | MS.gene28290 | -0.861395 | 1.06E-63 | -1.69E-46 |
| MS.gene052503 | MS.gene28290 | -0.827254 | 1.73E-54 | -1.69E-46 |
| MS.gene052506 | MS.gene28290 | -0.818082 | 2.36E-52 | -1.69E-46 |
| MS.gene052606 | MS.gene28290 | 0.811903 | 5.55E-51 | -1.69E-46 |
| MS.gene052822 | MS.gene28290 | -0.808008 | 3.83E-50 | -1.69E-46 |
| MS.gene052823 | MS.gene28290 | -0.826169 | 3.14E-54 | -1.69E-46 |
| MS.gene052825 | MS.gene28290 | -0.830131 | 3.49E-55 | -1.69E-46 |
| MS.gene052966 | MS.gene28290 | -0.837023 | 6.64E-57 | -1.69E-46 |
| MS.gene053203 | MS.gene28290 | -0.859941 | 2.92E-63 | -1.69E-46 |
| MS.gene053213 | MS.gene28290 | -0.810781 | 9.72E-51 | -1.69E-46 |
| MS.gene053286 | MS.gene28290 | -0.80241 | 5.69E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene28290 | 0.858736 | 6.71E-63 | -1.69E-46 |
| MS.gene053421 | MS.gene28290 | 0.812436 | 4.24E-51 | -1.69E-46 |
| MS.gene053480 | MS.gene28290 | -0.818661 | 1.74E-52 | -1.69E-46 |
| MS.gene053561 | MS.gene28290 | 0.809707 | 1.66E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene28290 | -0.806648 | 7.43E-50 | -1.69E-46 |
| MS.gene053579 | MS.gene28290 | -0.803153 | 3.99E-49 | -1.69E-46 |
| MS.gene053632 | MS.gene28290 | -0.813085 | 3.06E-51 | -1.69E-46 |
| MS.gene053824 | MS.gene28290 | -0.852402 | 4.73E-61 | -1.69E-46 |
| MS.gene053916 | MS.gene28290 | -0.818534 | 1.86E-52 | -1.69E-46 |
| MS.gene054003 | MS.gene28290 | -0.819965 | 8.80E-53 | -1.69E-46 |
| MS.gene054595 | MS.gene28290 | -0.833501 | 5.14E-56 | -1.69E-46 |
| MS.gene054600 | MS.gene28290 | -0.800518 | 1.39E-48 | -1.69E-46 |
| MS.gene054642 | MS.gene28290 | -0.825361 | 4.89E-54 | -1.69E-46 |
| MS.gene054706 | MS.gene28290 | 0.829107 | 6.19E-55 | -1.69E-46 |
| MS.gene054707 | MS.gene28290 | 0.818284 | 2.12E-52 | -1.69E-46 |
| MS.gene05470 | MS.gene28290 | -0.841853 | 3.69E-58 | -1.69E-46 |
| MS.gene054927 | MS.gene28290 | -0.803188 | 3.93E-49 | -1.69E-46 |
| MS.gene054954 | MS.gene28290 | -0.828931 | 6.83E-55 | -1.69E-46 |
| MS.gene055124 | MS.gene28290 | 0.805039 | 1.62E-49 | -1.69E-46 |
| MS.gene055154 | MS.gene28290 | 0.840179 | 1.02E-57 | -1.69E-46 |
| MS.gene055202 | MS.gene28290 | 0.818735 | 1.68E-52 | -1.69E-46 |
| MS.gene055206 | MS.gene28290 | -0.828646 | 8.01E-55 | -1.69E-46 |
| MS.gene055289 | MS.gene28290 | -0.804864 | 1.76E-49 | -1.69E-46 |
| MS.gene055362 | MS.gene28290 | -0.843966 | 1.01E-58 | -1.69E-46 |
| MS.gene055502 | MS.gene28290 | -0.871127 | 8.66E-67 | -1.69E-46 |
| MS.gene055577 | MS.gene28290 | -0.865782 | 4.60E-65 | -1.69E-46 |
| MS.gene055748 | MS.gene28290 | -0.822228 | 2.65E-53 | -1.69E-46 |
| MS.gene055750 | MS.gene28290 | -0.8374 | 5.32E-57 | -1.69E-46 |
| MS.gene055751 | MS.gene28290 | -0.823126 | 1.64E-53 | -1.69E-46 |
| MS.gene05589 | MS.gene28290 | -0.846908 | 1.62E-59 | -1.69E-46 |
| MS.gene055941 | MS.gene28290 | -0.834826 | 2.39E-56 | -1.69E-46 |
| MS.gene056311 | MS.gene28290 | 0.824612 | 7.35E-54 | -1.69E-46 |
| MS.gene056313 | MS.gene28290 | 0.810562 | 1.08E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene28290 | -0.86211 | 6.39E-64 | -1.69E-46 |
| MS.gene056510 | MS.gene28290 | -0.828109 | 1.08E-54 | -1.69E-46 |
| MS.gene056549 | MS.gene28290 | -0.824379 | 8.33E-54 | -1.69E-46 |
| MS.gene056550 | MS.gene28290 | 0.804644 | 1.96E-49 | -1.69E-46 |
| MS.gene056590 | MS.gene28290 | 0.806429 | 8.26E-50 | -1.69E-46 |
| MS.gene056591 | MS.gene28290 | 0.805504 | 1.29E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene28290 | -0.836236 | 1.05E-56 | -1.69E-46 |
| MS.gene056692 | MS.gene28290 | -0.8295 | 4.97E-55 | -1.69E-46 |
| MS.gene056856 | MS.gene28290 | 0.808928 | 2.43E-50 | -1.69E-46 |
| MS.gene056946 | MS.gene28290 | -0.810765 | 9.79E-51 | -1.69E-46 |
| MS.gene057014 | MS.gene28290 | 0.818089 | 2.35E-52 | -1.69E-46 |
| MS.gene057086 | MS.gene28290 | -0.800837 | 1.19E-48 | -1.69E-46 |
| MS.gene057416 | MS.gene28290 | -0.801295 | 9.63E-49 | -1.69E-46 |
| MS.gene057658 | MS.gene28290 | -0.803804 | 2.93E-49 | -1.69E-46 |
| MS.gene057768 | MS.gene28290 | 0.808602 | 2.86E-50 | -1.69E-46 |
| MS.gene057831 | MS.gene28290 | -0.816383 | 5.69E-52 | -1.69E-46 |
| MS.gene05787 | MS.gene28290 | -0.801276 | 9.72E-49 | -1.69E-46 |
| MS.gene057926 | MS.gene28290 | -0.805461 | 1.32E-49 | -1.69E-46 |
| MS.gene058068 | MS.gene28290 | 0.834777 | 2.46E-56 | -1.69E-46 |
| MS.gene058099 | MS.gene28290 | 0.805937 | 1.05E-49 | -1.69E-46 |
| MS.gene058109 | MS.gene28290 | 0.80275 | 4.84E-49 | -1.69E-46 |
| MS.gene058111 | MS.gene28290 | 0.808166 | 3.54E-50 | -1.69E-46 |
| MS.gene058117 | MS.gene28290 | 0.810402 | 1.17E-50 | -1.69E-46 |
| MS.gene058157 | MS.gene28290 | -0.833316 | 5.72E-56 | -1.69E-46 |
| MS.gene058171 | MS.gene28290 | 0.869481 | 3.00E-66 | -1.69E-46 |
| MS.gene058173 | MS.gene28290 | 0.860398 | 2.12E-63 | -1.69E-46 |
| MS.gene058175 | MS.gene28290 | 0.871414 | 6.96E-67 | -1.69E-46 |
| MS.gene058334 | MS.gene28290 | 0.819603 | 1.06E-52 | -1.69E-46 |
| MS.gene058338 | MS.gene28290 | -0.812314 | 4.51E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene28290 | -0.867359 | 1.45E-65 | -1.69E-46 |
| MS.gene058419 | MS.gene28290 | -0.856345 | 3.43E-62 | -1.69E-46 |
| MS.gene058544 | MS.gene28290 | -0.821719 | 3.48E-53 | -1.69E-46 |
| MS.gene058603 | MS.gene28290 | 0.809639 | 1.71E-50 | -1.69E-46 |
| MS.gene058773 | MS.gene28290 | -0.825088 | 5.67E-54 | -1.69E-46 |
| MS.gene058838 | MS.gene28290 | -0.820638 | 6.17E-53 | -1.69E-46 |
| MS.gene059022 | MS.gene28290 | -0.83985 | 1.24E-57 | -1.69E-46 |
| MS.gene059023 | MS.gene28290 | -0.850884 | 1.27E-60 | -1.69E-46 |
| MS.gene059045 | MS.gene28290 | 0.811037 | 8.55E-51 | -1.69E-46 |
| MS.gene05913 | MS.gene28290 | -0.802285 | 6.03E-49 | -1.69E-46 |
| MS.gene059223 | MS.gene28290 | 0.805069 | 1.59E-49 | -1.69E-46 |
| MS.gene05934 | MS.gene28290 | -0.829863 | 4.06E-55 | -1.69E-46 |
| MS.gene059367 | MS.gene28290 | -0.811149 | 8.09E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene28290 | -0.835873 | 1.30E-56 | -1.69E-46 |
| MS.gene059703 | MS.gene28290 | 0.803962 | 2.71E-49 | -1.69E-46 |
| MS.gene059718 | MS.gene28290 | -0.845709 | 3.43E-59 | -1.69E-46 |
| MS.gene059756 | MS.gene28290 | -0.819954 | 8.85E-53 | -1.69E-46 |
| MS.gene059801 | MS.gene28290 | -0.821197 | 4.59E-53 | -1.69E-46 |
| MS.gene059865 | MS.gene28290 | -0.834874 | 2.33E-56 | -1.69E-46 |
| MS.gene059902 | MS.gene28290 | -0.847313 | 1.25E-59 | -1.69E-46 |
| MS.gene06000 | MS.gene28290 | -0.805234 | 1.47E-49 | -1.69E-46 |
| MS.gene060595 | MS.gene28290 | 0.832485 | 9.20E-56 | -1.69E-46 |
| MS.gene060703 | MS.gene28290 | -0.808041 | 3.76E-50 | -1.69E-46 |
| MS.gene060762 | MS.gene28290 | -0.868599 | 5.79E-66 | -1.69E-46 |
| MS.gene060778 | MS.gene28290 | -0.832629 | 8.48E-56 | -1.69E-46 |
| MS.gene06077 | MS.gene28290 | -0.858472 | 8.05E-63 | -1.69E-46 |
| MS.gene06092 | MS.gene28290 | -0.815453 | 9.17E-52 | -1.69E-46 |
| MS.gene060982 | MS.gene28290 | -0.832287 | 1.03E-55 | -1.69E-46 |
| MS.gene061044 | MS.gene28290 | 0.818583 | 1.82E-52 | -1.69E-46 |
| MS.gene061150 | MS.gene28290 | -0.810903 | 9.14E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28290.t1 | MTR_2g025080 | 94.086 | 186 | 11 | 0 | 1 | 186 | 1 | 186 | 1.21e-118 | 335 |
| MS.gene28290.t1 | MTR_4g132760 | 58.772 | 228 | 68 | 6 | 1 | 213 | 1 | 217 | 1.50e-75 | 227 |
| MS.gene28290.t1 | MTR_8g013650 | 79.592 | 49 | 10 | 0 | 165 | 213 | 186 | 234 | 2.28e-25 | 99.4 |
| MS.gene28290.t1 | MTR_7g056407 | 47.525 | 101 | 39 | 1 | 127 | 213 | 239 | 339 | 7.80e-22 | 91.7 |
| MS.gene28290.t1 | MTR_3g065180 | 53.846 | 52 | 24 | 0 | 162 | 213 | 289 | 340 | 3.30e-16 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28290.t1 | AT4G03965 | 53.604 | 222 | 81 | 6 | 1 | 213 | 1 | 209 | 6.26e-67 | 205 |
| MS.gene28290.t1 | AT4G22250 | 51.485 | 202 | 79 | 7 | 21 | 213 | 23 | 214 | 3.37e-58 | 183 |
| MS.gene28290.t1 | AT1G62370 | 38.767 | 227 | 102 | 8 | 1 | 213 | 1 | 204 | 2.28e-41 | 139 |
| MS.gene28290.t1 | AT3G25030 | 51.538 | 130 | 49 | 3 | 91 | 213 | 88 | 210 | 4.48e-35 | 124 |
| MS.gene28290.t1 | AT3G25030 | 51.538 | 130 | 49 | 3 | 91 | 213 | 128 | 250 | 2.11e-34 | 123 |
| MS.gene28290.t1 | AT3G25030 | 51.538 | 130 | 49 | 3 | 91 | 213 | 128 | 250 | 2.11e-34 | 123 |
| MS.gene28290.t1 | AT3G25030 | 51.538 | 130 | 49 | 3 | 91 | 213 | 128 | 250 | 2.11e-34 | 123 |
| MS.gene28290.t1 | AT4G13100 | 45.736 | 129 | 38 | 4 | 99 | 213 | 146 | 256 | 3.11e-27 | 104 |
| MS.gene28290.t1 | AT4G13100 | 45.736 | 129 | 38 | 4 | 99 | 213 | 146 | 256 | 3.11e-27 | 104 |
| MS.gene28290.t1 | AT4G13100 | 45.736 | 129 | 38 | 4 | 99 | 213 | 155 | 265 | 4.33e-27 | 104 |
| MS.gene28290.t1 | AT4G13100 | 45.736 | 129 | 41 | 4 | 99 | 213 | 143 | 256 | 4.60e-27 | 104 |
| MS.gene28290.t1 | AT4G13100 | 45.736 | 129 | 41 | 4 | 99 | 213 | 146 | 259 | 5.15e-27 | 103 |
| MS.gene28290.t1 | AT4G13100 | 45.736 | 129 | 41 | 4 | 99 | 213 | 146 | 259 | 5.15e-27 | 103 |
| MS.gene28290.t1 | AT4G13100 | 45.736 | 129 | 41 | 4 | 99 | 213 | 146 | 259 | 5.15e-27 | 103 |
| MS.gene28290.t1 | AT4G13100 | 45.736 | 129 | 41 | 4 | 99 | 213 | 146 | 259 | 5.15e-27 | 103 |
| MS.gene28290.t1 | AT3G07120 | 57.143 | 49 | 21 | 0 | 165 | 213 | 312 | 360 | 3.66e-16 | 76.3 |
Find 77 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGAACGGTTTGGATTTAT+TGG | 0.182452 | 2.1:-64696148 | MS.gene28290:CDS |
| TTGGATGCTGTGGGGCCAAT+TGG | 0.283458 | 2.1:-64696129 | MS.gene28290:CDS |
| TGGATGCTGTGGGGCCAATT+GGG | 0.286154 | 2.1:-64696128 | MS.gene28290:CDS |
| CAACGATTCTCGGTTTGTTA+AGG | 0.332079 | 2.1:-64696219 | MS.gene28290:CDS |
| CGGTGATACGGTGGCGGTTA+AGG | 0.338807 | 2.1:-64695846 | MS.gene28290:CDS |
| AGCAGAGAGGGGAGGATGCC+AGG | 0.341804 | 2.1:-64695917 | MS.gene28290:CDS |
| AACGATTCTCGGTTTGTTAA+GGG | 0.364073 | 2.1:-64696218 | MS.gene28290:CDS |
| CGGAGTTTCAAGGAACGGTT+TGG | 0.366789 | 2.1:-64696157 | MS.gene28290:CDS |
| GTTTGTATGGGGAGGAATAA+AGG | 0.376604 | 2.1:-64695776 | MS.gene28290:CDS |
| CTGCTACTCGTTTCCGCCGC+TGG | 0.393440 | 2.1:+64695935 | None:intergenic |
| TTCAGTGTGTTGCGTTTGTA+TGG | 0.395970 | 2.1:-64695789 | MS.gene28290:CDS |
| CGGTTTGTTAAGGGAACAAA+TGG | 0.406995 | 2.1:-64696209 | MS.gene28290:CDS |
| ACTCAGTCAACAACGATTCT+CGG | 0.410134 | 2.1:-64696229 | MS.gene28290:CDS |
| CGGTTTATTATTGCAAGAAC+CGG | 0.412259 | 2.1:-64696263 | MS.gene28290:CDS |
| TCTTGCTGCGGCGTTGGCGG+CGG | 0.414122 | 2.1:-64695975 | MS.gene28290:CDS |
| ACCGGAGCCACACACATATT+CGG | 0.415523 | 2.1:+64696031 | None:intergenic |
| GAATCTTGCTGCGGCGTTGG+CGG | 0.416368 | 2.1:-64695978 | MS.gene28290:CDS |
| GACACTCTCCACGGCGTCCC+TGG | 0.418136 | 2.1:+64695899 | None:intergenic |
| GACAATAGAACGATTACAAA+GGG | 0.451004 | 2.1:+64695676 | None:intergenic |
| TCGTTTCCGCCGCTGGTCCT+CGG | 0.452026 | 2.1:+64695942 | None:intergenic |
| TATGAATCTTGCTGCGGCGT+TGG | 0.458195 | 2.1:-64695981 | MS.gene28290:CDS |
| TTGGATTTATTGGATGCTGT+GGG | 0.466650 | 2.1:-64696138 | MS.gene28290:CDS |
| GGCGGTTAAGGCGGAGGAGA+AGG | 0.469647 | 2.1:-64695834 | MS.gene28290:CDS |
| AGTGTCGTTGATGCGGTTGT+TGG | 0.470381 | 2.1:-64695882 | MS.gene28290:CDS |
| GGATGCTGTGGGGCCAATTG+GGG | 0.478261 | 2.1:-64696127 | MS.gene28290:CDS |
| GCGGAAACGAGTAGCAGAGA+GGG | 0.496963 | 2.1:-64695929 | MS.gene28290:CDS |
| CTCGAACCAGTTTGAATCAC+CGG | 0.499393 | 2.1:+64696013 | None:intergenic |
| GAACAAATGGACGGTGTTGA+TGG | 0.500960 | 2.1:-64696196 | MS.gene28290:CDS |
| GATGCGGTTGTTGGAGGAAA+CGG | 0.502011 | 2.1:-64695873 | MS.gene28290:CDS |
| TTTGGATTTATTGGATGCTG+TGG | 0.505006 | 2.1:-64696139 | MS.gene28290:CDS |
| GACTGAGTTTGAGACTGAAC+CGG | 0.505067 | 2.1:+64696244 | None:intergenic |
| AGAAGAACGGAATCCCCAAT+TGG | 0.510016 | 2.1:+64696114 | None:intergenic |
| TCAGTGTGTTGCGTTTGTAT+GGG | 0.512290 | 2.1:-64695788 | MS.gene28290:CDS |
| TACGGTGGCGGTTAAGGCGG+AGG | 0.514169 | 2.1:-64695840 | MS.gene28290:CDS |
| TCCGAATATGTGTGTGGCTC+CGG | 0.514268 | 2.1:-64696032 | MS.gene28290:CDS |
| GCAGAGAGGGGAGGATGCCA+GGG | 0.514827 | 2.1:-64695916 | MS.gene28290:CDS |
| AGGGAGCTGTGGTTGAATAG+AGG | 0.515714 | 2.1:-64695707 | MS.gene28290:CDS |
| CACATATTCGGATCTTCACT+CGG | 0.516936 | 2.1:+64696043 | None:intergenic |
| GTGGCTCCGGTGATTCAAAC+TGG | 0.523146 | 2.1:-64696019 | MS.gene28290:CDS |
| ACGTCGGAGACGGAGTTTCA+AGG | 0.523784 | 2.1:-64696167 | MS.gene28290:CDS |
| GTCGTTGATGCGGTTGTTGG+AGG | 0.526999 | 2.1:-64695879 | MS.gene28290:CDS |
| GAGAAGGTTGATGCTGTGGC+GGG | 0.541318 | 2.1:-64695818 | MS.gene28290:CDS |
| TCAGCCACGGATTCTTGATA+CGG | 0.563090 | 2.1:-64696068 | MS.gene28290:CDS |
| GGAGGATGCCAGGGACGCCG+TGG | 0.564167 | 2.1:-64695907 | MS.gene28290:CDS |
| ATGGTGTGCCACGTCGGAGA+CGG | 0.568575 | 2.1:-64696177 | MS.gene28290:CDS |
| CAGTGTGTTGCGTTTGTATG+GGG | 0.572597 | 2.1:-64695787 | MS.gene28290:CDS |
| GGAGAAGGTTGATGCTGTGG+CGG | 0.584424 | 2.1:-64695819 | MS.gene28290:CDS |
| GAGTTTGTTCTAGGGAGCTG+TGG | 0.587508 | 2.1:-64695718 | MS.gene28290:CDS |
| GGAAACGGAAAACGGTGATA+CGG | 0.591037 | 2.1:-64695858 | MS.gene28290:CDS |
| TGATACGGTGGCGGTTAAGG+CGG | 0.595277 | 2.1:-64695843 | MS.gene28290:CDS |
| TGGATTTATTGGATGCTGTG+GGG | 0.595995 | 2.1:-64696137 | MS.gene28290:CDS |
| GGAGGAGAAGGTTGATGCTG+TGG | 0.597954 | 2.1:-64695822 | MS.gene28290:CDS |
| TTGTTGGAGGAAACGGAAAA+CGG | 0.606520 | 2.1:-64695866 | MS.gene28290:CDS |
| TTCGAGTATGAATCTTGCTG+CGG | 0.609891 | 2.1:-64695987 | MS.gene28290:CDS |
| CGACAATAGAACGATTACAA+AGG | 0.610611 | 2.1:+64695675 | None:intergenic |
| GGAGACGGAGTTTCAAGGAA+CGG | 0.610966 | 2.1:-64696162 | MS.gene28290:CDS |
| CGCATCAACGACACTCTCCA+CGG | 0.617567 | 2.1:+64695890 | None:intergenic |
| AAGACAACTCCGAGGACCAG+CGG | 0.621097 | 2.1:-64695951 | MS.gene28290:CDS |
| GGCGGAAACGAGTAGCAGAG+AGG | 0.626935 | 2.1:-64695930 | MS.gene28290:CDS |
| GAGAGAAATATGAGTCAACT+CGG | 0.630187 | 2.1:-64696283 | None:intergenic |
| CGGAAACGAGTAGCAGAGAG+GGG | 0.639986 | 2.1:-64695928 | MS.gene28290:CDS |
| GGTGCGGCGTTTATTCCATG+TGG | 0.646281 | 2.1:-64695755 | MS.gene28290:CDS |
| TTGAAACTCCGTCTCCGACG+TGG | 0.647313 | 2.1:+64696169 | None:intergenic |
| TGTGTTGCGTTTGTATGGGG+AGG | 0.653722 | 2.1:-64695784 | MS.gene28290:CDS |
| ACAACTCCGAGGACCAGCGG+CGG | 0.656277 | 2.1:-64695948 | MS.gene28290:CDS |
| TTGTTAAGGGAACAAATGGA+CGG | 0.656465 | 2.1:-64696205 | MS.gene28290:CDS |
| GGAAAACGGTGATACGGTGG+CGG | 0.658656 | 2.1:-64695852 | MS.gene28290:CDS |
| AAACGAGTAGCAGAGAGGGG+AGG | 0.665056 | 2.1:-64695925 | MS.gene28290:CDS |
| CGTGGAGAGTGTCGTTGATG+CGG | 0.674817 | 2.1:-64695889 | MS.gene28290:CDS |
| AACGGAAAACGGTGATACGG+TGG | 0.698643 | 2.1:-64695855 | MS.gene28290:CDS |
| AATCACAACAGAATCAGCCA+CGG | 0.698955 | 2.1:-64696081 | MS.gene28290:CDS |
| TATGGGGAGGAATAAAGGTG+CGG | 0.702799 | 2.1:-64695771 | MS.gene28290:CDS |
| GTGTTGATGGTGTGCCACGT+CGG | 0.713199 | 2.1:-64696183 | MS.gene28290:CDS |
| GGATCCGTATCAAGAATCCG+TGG | 0.721967 | 2.1:+64696064 | None:intergenic |
| ACAATAGAACGATTACAAAG+GGG | 0.737571 | 2.1:+64695677 | None:intergenic |
| TGAAGATCCGAATATGTGTG+TGG | 0.744872 | 2.1:-64696038 | MS.gene28290:CDS |
| GCGGCGGAAAGACAACTCCG+AGG | 0.786602 | 2.1:-64695959 | MS.gene28290:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAATAGAACGATTACAAAG+GGG | + | chr2.1:64696255-64696274 | None:intergenic | 30.0% | |
| ATTCAGAAGAAGAAGAAGAA+CGG | + | chr2.1:64695831-64695850 | None:intergenic | 30.0% | |
| GACAATAGAACGATTACAAA+GGG | + | chr2.1:64696256-64696275 | None:intergenic | 30.0% | |
| !! | TTTTTGCAGAGTTTGTTCTA+GGG | - | chr2.1:64696203-64696222 | MS.gene28290:CDS | 30.0% |
| CGACAATAGAACGATTACAA+AGG | + | chr2.1:64696257-64696276 | None:intergenic | 35.0% | |
| CGGTTTATTATTGCAAGAAC+CGG | - | chr2.1:64695666-64695685 | MS.gene28290:CDS | 35.0% | |
| TTGGATTTATTGGATGCTGT+GGG | - | chr2.1:64695791-64695810 | MS.gene28290:CDS | 35.0% | |
| TTGTTAAGGGAACAAATGGA+CGG | - | chr2.1:64695724-64695743 | MS.gene28290:CDS | 35.0% | |
| TTTGGATTTATTGGATGCTG+TGG | - | chr2.1:64695790-64695809 | MS.gene28290:CDS | 35.0% | |
| ! | AAGGAACGGTTTGGATTTAT+TGG | - | chr2.1:64695781-64695800 | MS.gene28290:CDS | 35.0% |
| !! | AACGATTCTCGGTTTGTTAA+GGG | - | chr2.1:64695711-64695730 | MS.gene28290:CDS | 35.0% |
| !! | CTTTTTGCAGAGTTTGTTCT+AGG | - | chr2.1:64696202-64696221 | MS.gene28290:CDS | 35.0% |
| AATCACAACAGAATCAGCCA+CGG | - | chr2.1:64695848-64695867 | MS.gene28290:CDS | 40.0% | |
| ACTCAGTCAACAACGATTCT+CGG | - | chr2.1:64695700-64695719 | MS.gene28290:CDS | 40.0% | |
| CACATATTCGGATCTTCACT+CGG | + | chr2.1:64695889-64695908 | None:intergenic | 40.0% | |
| GTTTGTATGGGGAGGAATAA+AGG | - | chr2.1:64696153-64696172 | MS.gene28290:CDS | 40.0% | |
| TCAGTGTGTTGCGTTTGTAT+GGG | - | chr2.1:64696141-64696160 | MS.gene28290:CDS | 40.0% | |
| TGAAGATCCGAATATGTGTG+TGG | - | chr2.1:64695891-64695910 | MS.gene28290:CDS | 40.0% | |
| TGGATTTATTGGATGCTGTG+GGG | - | chr2.1:64695792-64695811 | MS.gene28290:CDS | 40.0% | |
| TTCAGTGTGTTGCGTTTGTA+TGG | - | chr2.1:64696140-64696159 | MS.gene28290:CDS | 40.0% | |
| TTCGAGTATGAATCTTGCTG+CGG | - | chr2.1:64695942-64695961 | MS.gene28290:CDS | 40.0% | |
| TTGTTGGAGGAAACGGAAAA+CGG | - | chr2.1:64696063-64696082 | MS.gene28290:CDS | 40.0% | |
| ! | CTGCAAAAAGTATGTCCACA+TGG | + | chr2.1:64696192-64696211 | None:intergenic | 40.0% |
| !! | CAACGATTCTCGGTTTGTTA+AGG | - | chr2.1:64695710-64695729 | MS.gene28290:CDS | 40.0% |
| !! | CGGTTTGTTAAGGGAACAAA+TGG | - | chr2.1:64695720-64695739 | MS.gene28290:CDS | 40.0% |
| AGAAGAACGGAATCCCCAAT+TGG | + | chr2.1:64695818-64695837 | None:intergenic | 45.0% | |
| CAGTGTGTTGCGTTTGTATG+GGG | - | chr2.1:64696142-64696161 | MS.gene28290:CDS | 45.0% | |
| CTCGAACCAGTTTGAATCAC+CGG | + | chr2.1:64695919-64695938 | None:intergenic | 45.0% | |
| GGAAACGGAAAACGGTGATA+CGG | - | chr2.1:64696071-64696090 | MS.gene28290:CDS | 45.0% | |
| TATGGGGAGGAATAAAGGTG+CGG | - | chr2.1:64696158-64696177 | MS.gene28290:CDS | 45.0% | |
| TCAGCCACGGATTCTTGATA+CGG | - | chr2.1:64695861-64695880 | MS.gene28290:CDS | 45.0% | |
| ! | GAACAAATGGACGGTGTTGA+TGG | - | chr2.1:64695733-64695752 | MS.gene28290:CDS | 45.0% |
| ! | GACTGAGTTTGAGACTGAAC+CGG | + | chr2.1:64695688-64695707 | None:intergenic | 45.0% |
| AACGGAAAACGGTGATACGG+TGG | - | chr2.1:64696074-64696093 | MS.gene28290:CDS | 50.0% | |
| ACCGGAGCCACACACATATT+CGG | + | chr2.1:64695901-64695920 | None:intergenic | 50.0% | |
| AGGGAGCTGTGGTTGAATAG+AGG | - | chr2.1:64696222-64696241 | MS.gene28290:CDS | 50.0% | |
| CGGAGTTTCAAGGAACGGTT+TGG | - | chr2.1:64695772-64695791 | MS.gene28290:CDS | 50.0% | |
| GATGCGGTTGTTGGAGGAAA+CGG | - | chr2.1:64696056-64696075 | MS.gene28290:CDS | 50.0% | |
| GGAGACGGAGTTTCAAGGAA+CGG | - | chr2.1:64695767-64695786 | MS.gene28290:CDS | 50.0% | |
| GGATCCGTATCAAGAATCCG+TGG | + | chr2.1:64695868-64695887 | None:intergenic | 50.0% | |
| TATGAATCTTGCTGCGGCGT+TGG | - | chr2.1:64695948-64695967 | MS.gene28290:CDS | 50.0% | |
| TCCGAATATGTGTGTGGCTC+CGG | - | chr2.1:64695897-64695916 | MS.gene28290:CDS | 50.0% | |
| TGTGTTGCGTTTGTATGGGG+AGG | - | chr2.1:64696145-64696164 | MS.gene28290:CDS | 50.0% | |
| ! | AGTGTCGTTGATGCGGTTGT+TGG | - | chr2.1:64696047-64696066 | MS.gene28290:CDS | 50.0% |
| !! | GAGTTTGTTCTAGGGAGCTG+TGG | - | chr2.1:64696211-64696230 | MS.gene28290:CDS | 50.0% |
| AAACGAGTAGCAGAGAGGGG+AGG | - | chr2.1:64696004-64696023 | MS.gene28290:CDS | 55.0% | |
| AAGACAACTCCGAGGACCAG+CGG | - | chr2.1:64695978-64695997 | MS.gene28290:CDS | 55.0% | |
| ACGTCGGAGACGGAGTTTCA+AGG | - | chr2.1:64695762-64695781 | MS.gene28290:CDS | 55.0% | |
| CGCATCAACGACACTCTCCA+CGG | + | chr2.1:64696042-64696061 | None:intergenic | 55.0% | |
| CGGAAACGAGTAGCAGAGAG+GGG | - | chr2.1:64696001-64696020 | MS.gene28290:CDS | 55.0% | |
| GCGGAAACGAGTAGCAGAGA+GGG | - | chr2.1:64696000-64696019 | MS.gene28290:CDS | 55.0% | |
| GGAAAACGGTGATACGGTGG+CGG | - | chr2.1:64696077-64696096 | MS.gene28290:CDS | 55.0% | |
| GGTGCGGCGTTTATTCCATG+TGG | - | chr2.1:64696174-64696193 | MS.gene28290:CDS | 55.0% | |
| GTCGTTGATGCGGTTGTTGG+AGG | - | chr2.1:64696050-64696069 | MS.gene28290:CDS | 55.0% | |
| GTGGCTCCGGTGATTCAAAC+TGG | - | chr2.1:64695910-64695929 | MS.gene28290:CDS | 55.0% | |
| TGGATGCTGTGGGGCCAATT+GGG | - | chr2.1:64695801-64695820 | MS.gene28290:CDS | 55.0% | |
| TTGAAACTCCGTCTCCGACG+TGG | + | chr2.1:64695763-64695782 | None:intergenic | 55.0% | |
| TTGGATGCTGTGGGGCCAAT+TGG | - | chr2.1:64695800-64695819 | MS.gene28290:CDS | 55.0% | |
| ! | TGATACGGTGGCGGTTAAGG+CGG | - | chr2.1:64696086-64696105 | MS.gene28290:CDS | 55.0% |
| !! | CGTGGAGAGTGTCGTTGATG+CGG | - | chr2.1:64696040-64696059 | MS.gene28290:CDS | 55.0% |
| !! | GAGAAGGTTGATGCTGTGGC+GGG | - | chr2.1:64696111-64696130 | MS.gene28290:CDS | 55.0% |
| !! | GGAGAAGGTTGATGCTGTGG+CGG | - | chr2.1:64696110-64696129 | MS.gene28290:CDS | 55.0% |
| !! | GGAGGAGAAGGTTGATGCTG+TGG | - | chr2.1:64696107-64696126 | MS.gene28290:CDS | 55.0% |
| !! | GTGTTGATGGTGTGCCACGT+CGG | - | chr2.1:64695746-64695765 | MS.gene28290:CDS | 55.0% |
| ATGGTGTGCCACGTCGGAGA+CGG | - | chr2.1:64695752-64695771 | MS.gene28290:CDS | 60.0% | |
| GGATGCTGTGGGGCCAATTG+GGG | - | chr2.1:64695802-64695821 | MS.gene28290:CDS | 60.0% | |
| GGCGGAAACGAGTAGCAGAG+AGG | - | chr2.1:64695999-64696018 | MS.gene28290:CDS | 60.0% | |
| ! | CGGTGATACGGTGGCGGTTA+AGG | - | chr2.1:64696083-64696102 | MS.gene28290:CDS | 60.0% |
| ! | GAATCTTGCTGCGGCGTTGG+CGG | - | chr2.1:64695951-64695970 | MS.gene28290:CDS | 60.0% |
| ACAACTCCGAGGACCAGCGG+CGG | - | chr2.1:64695981-64696000 | MS.gene28290:CDS | 65.0% | |
| AGCAGAGAGGGGAGGATGCC+AGG | - | chr2.1:64696012-64696031 | MS.gene28290:CDS | 65.0% | |
| CTGCTACTCGTTTCCGCCGC+TGG | + | chr2.1:64695997-64696016 | None:intergenic | 65.0% | |
| GCAGAGAGGGGAGGATGCCA+GGG | - | chr2.1:64696013-64696032 | MS.gene28290:CDS | 65.0% | |
| GCGGCGGAAAGACAACTCCG+AGG | - | chr2.1:64695970-64695989 | MS.gene28290:CDS | 65.0% | |
| GGCGGTTAAGGCGGAGGAGA+AGG | - | chr2.1:64696095-64696114 | MS.gene28290:CDS | 65.0% | |
| TCGTTTCCGCCGCTGGTCCT+CGG | + | chr2.1:64695990-64696009 | None:intergenic | 65.0% | |
| ! | TACGGTGGCGGTTAAGGCGG+AGG | - | chr2.1:64696089-64696108 | MS.gene28290:CDS | 65.0% |
| GACACTCTCCACGGCGTCCC+TGG | + | chr2.1:64696033-64696052 | None:intergenic | 70.0% | |
| !! | TCTTGCTGCGGCGTTGGCGG+CGG | - | chr2.1:64695954-64695973 | MS.gene28290:CDS | 70.0% |
| GGAGGATGCCAGGGACGCCG+TGG | - | chr2.1:64696022-64696041 | MS.gene28290:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 64695655 | 64696296 | 64695655 | ID=MS.gene28290 |
| chr2.1 | mRNA | 64695655 | 64696296 | 64695655 | ID=MS.gene28290.t1;Parent=MS.gene28290 |
| chr2.1 | exon | 64695655 | 64696296 | 64695655 | ID=MS.gene28290.t1.exon1;Parent=MS.gene28290.t1 |
| chr2.1 | CDS | 64695655 | 64696296 | 64695655 | ID=cds.MS.gene28290.t1;Parent=MS.gene28290.t1 |
| Gene Sequence |
| Protein sequence |
