AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene41873


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41873.t1 MTR_7g088210 97.297 74 2 0 13 86 179 252 2.55e-48 153
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41873.t1 AT2G41120 46.835 79 34 2 13 85 181 257 8.28e-16 69.7

Find 23 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAGATCCTACCAACTTA+TGG 0.295152 7.4:-20158205 MS.gene41873:CDS
ATTTCATCACCTATTCAATC+AGG 0.383372 7.4:-20158753 MS.gene41873:intron
TATTGAAAACCTGATTGAAT+AGG 0.413740 7.4:+20158744 None:intergenic
GAAAGATCCTACCAACTTAT+GGG 0.416370 7.4:-20158204 MS.gene41873:CDS
CTCCAAGGGCAAAGCTTCCC+AGG 0.433451 7.4:-20158080 MS.gene41873:CDS
TACATTGTGTTCTGCCCTCA+AGG 0.434776 7.4:-20158123 MS.gene41873:CDS
CTTTGCCCTTGGAGCTTCAG+TGG 0.439060 7.4:+20158089 None:intergenic
CGCGTATATGATCTAGAACT+TGG 0.442298 7.4:-20158162 MS.gene41873:CDS
AGCCTGGGAAGCTTTGCCCT+TGG 0.483988 7.4:+20158078 None:intergenic
TGGCATCATTAGACCCTTGA+GGG 0.509241 7.4:+20158109 None:intergenic
TTCTGAGGTAGCATTAAGCC+TGG 0.518926 7.4:+20158062 None:intergenic
CAGCATATTCTCCCATAAGT+TGG 0.529568 7.4:+20158193 None:intergenic
GGAATACTGCAATGTGCAGT+TGG 0.536785 7.4:-20158776 None:intergenic
CTTATGGGAGAATATGCTGC+AGG 0.540524 7.4:-20158189 MS.gene41873:CDS
TGATGATGTTTGTGTTGCAA+TGG 0.550109 7.4:-20158235 MS.gene41873:CDS
TGATGCCACTGAAGCTCCAA+GGG 0.576853 7.4:-20158094 MS.gene41873:CDS
ACATTGTGTTCTGCCCTCAA+GGG 0.584678 7.4:-20158122 MS.gene41873:CDS
ATGATGCCACTGAAGCTCCA+AGG 0.592193 7.4:-20158095 MS.gene41873:CDS
ATATTCTCCCATAAGTTGGT+AGG 0.594558 7.4:+20158197 None:intergenic
GTGGCATCATTAGACCCTTG+AGG 0.624422 7.4:+20158108 None:intergenic
ATATGCTACAAGATGTTCTG+AGG 0.636965 7.4:+20158047 None:intergenic
TCTGAGGTAGCATTAAGCCT+GGG 0.648996 7.4:+20158063 None:intergenic
TTATGGGAGAATATGCTGCA+GGG 0.653319 7.4:-20158188 MS.gene41873:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTCATTTTATTTTTAG+TGG - chr7.4:20158098-20158117 MS.gene41873:CDS 15.0%
!! ATATGATACATAACATTTCA+AGG + chr7.4:20158357-20158376 None:intergenic 20.0%
!! ATTCACAAATGTTATATGAT+CGG - chr7.4:20158499-20158518 MS.gene41873:intron 20.0%
!! GAATTAAATCTAATCTTGAA+TGG + chr7.4:20158483-20158502 None:intergenic 20.0%
!!! TCATTTTATTTTTAGTGGTA+TGG - chr7.4:20158103-20158122 MS.gene41873:CDS 20.0%
! TATTGAAAACCTGATTGAAT+AGG + chr7.4:20158059-20158078 None:intergenic 25.0%
! TCTTTCTCAAACTTAAGAAA+CGG + chr7.4:20158267-20158286 None:intergenic 25.0%
! TGCTGAATATAGAATCATAA+AGG - chr7.4:20158180-20158199 MS.gene41873:CDS 25.0%
!!! AAATGTTATATGATCGGTTT+TGG - chr7.4:20158505-20158524 MS.gene41873:intron 25.0%
!!! TACTGAGTTCAATTTTGATT+CGG + chr7.4:20158404-20158423 None:intergenic 25.0%
!!! TTTTTAACTTGAATTTGAGC+AGG - chr7.4:20158539-20158558 MS.gene41873:intron 25.0%
ATCTAATCTTGAATGGTAAC+AGG + chr7.4:20158476-20158495 None:intergenic 30.0%
ATTTCATCACCTATTCAATC+AGG - chr7.4:20158047-20158066 MS.gene41873:CDS 30.0%
GATACATAACATTTCAAGGT+TGG + chr7.4:20158353-20158372 None:intergenic 30.0%
GCTATGTAAATTGAGAAAGA+TGG - chr7.4:20158227-20158246 MS.gene41873:CDS 30.0%
! GAAAGATATTTCAGCAGTTT+TGG - chr7.4:20158281-20158300 MS.gene41873:intron 30.0%
!!! TTTAGTGGTATGGTTTTGTT+TGG - chr7.4:20158113-20158132 MS.gene41873:CDS 30.0%
AACTTAAGAAACGGTCCATT+AGG + chr7.4:20158258-20158277 None:intergenic 35.0%
ACTTAAGAAACGGTCCATTA+GGG + chr7.4:20158257-20158276 None:intergenic 35.0%
AGAAAGATCCTACCAACTTA+TGG - chr7.4:20158595-20158614 MS.gene41873:intron 35.0%
ATATGCTACAAGATGTTCTG+AGG + chr7.4:20158756-20158775 None:intergenic 35.0%
GAAAGATCCTACCAACTTAT+GGG - chr7.4:20158596-20158615 MS.gene41873:intron 35.0%
GAATCATAAAGGAGCTATGA+TGG - chr7.4:20158191-20158210 MS.gene41873:CDS 35.0%
! TGATGATGTTTGTGTTGCAA+TGG - chr7.4:20158565-20158584 MS.gene41873:intron 35.0%
!! ATATTCTCCCATAAGTTGGT+AGG + chr7.4:20158606-20158625 None:intergenic 35.0%
AGAAAGATGGAGTTCCCTAA+TGG - chr7.4:20158240-20158259 MS.gene41873:CDS 40.0%
CAGCATATTCTCCCATAAGT+TGG + chr7.4:20158610-20158629 None:intergenic 40.0%
TTATGGGAGAATATGCTGCA+GGG - chr7.4:20158612-20158631 MS.gene41873:intron 40.0%
! AAAGGAGCTATGATGGAGTT+AGG - chr7.4:20158198-20158217 MS.gene41873:CDS 40.0%
! AAGGAGCTATGATGGAGTTA+GGG - chr7.4:20158199-20158218 MS.gene41873:CDS 40.0%
! CGCGTATATGATCTAGAACT+TGG - chr7.4:20158638-20158657 MS.gene41873:intron 40.0%
ACATTGTGTTCTGCCCTCAA+GGG - chr7.4:20158678-20158697 MS.gene41873:intron 45.0%
CAGCTAACTCTATCTGTGTC+TGG + chr7.4:20158317-20158336 None:intergenic 45.0%
CTTATGGGAGAATATGCTGC+AGG - chr7.4:20158611-20158630 MS.gene41873:intron 45.0%
TACATTGTGTTCTGCCCTCA+AGG - chr7.4:20158677-20158696 MS.gene41873:intron 45.0%
TGGCATCATTAGACCCTTGA+GGG + chr7.4:20158694-20158713 None:intergenic 45.0%
! AGGAGCTATGATGGAGTTAG+GGG - chr7.4:20158200-20158219 MS.gene41873:CDS 45.0%
! TCTGAGGTAGCATTAAGCCT+GGG + chr7.4:20158740-20158759 None:intergenic 45.0%
!! TTCTGAGGTAGCATTAAGCC+TGG + chr7.4:20158741-20158760 None:intergenic 45.0%
ATGATGCCACTGAAGCTCCA+AGG - chr7.4:20158705-20158724 MS.gene41873:intron 50.0%
GTGGCATCATTAGACCCTTG+AGG + chr7.4:20158695-20158714 None:intergenic 50.0%
TGATGCCACTGAAGCTCCAA+GGG - chr7.4:20158706-20158725 MS.gene41873:intron 50.0%
! AGCTATGATGGAGTTAGGGG+AGG - chr7.4:20158203-20158222 MS.gene41873:CDS 50.0%
! CTATGATGGAGTTAGGGGAG+GGG - chr7.4:20158205-20158224 MS.gene41873:CDS 50.0%
! GCTATGATGGAGTTAGGGGA+GGG - chr7.4:20158204-20158223 MS.gene41873:CDS 50.0%
CTTTGCCCTTGGAGCTTCAG+TGG + chr7.4:20158714-20158733 None:intergenic 55.0%
AGCCTGGGAAGCTTTGCCCT+TGG + chr7.4:20158725-20158744 None:intergenic 60.0%
CTCCAAGGGCAAAGCTTCCC+AGG - chr7.4:20158720-20158739 MS.gene41873:intron 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 20158035 20158787 20158035 ID=MS.gene41873
chr7.4 mRNA 20158035 20158787 20158035 ID=MS.gene41873.t1;Parent=MS.gene41873
chr7.4 exon 20158754 20158787 20158754 ID=MS.gene41873.t1.exon1;Parent=MS.gene41873.t1
chr7.4 CDS 20158754 20158787 20158754 ID=cds.MS.gene41873.t1;Parent=MS.gene41873.t1
chr7.4 exon 20158035 20158261 20158035 ID=MS.gene41873.t1.exon2;Parent=MS.gene41873.t1
chr7.4 CDS 20158035 20158261 20158035 ID=cds.MS.gene41873.t1;Parent=MS.gene41873.t1
Gene Sequence

>MS.gene41873

ATGTGCAGTTGGATTTCATCACCTATTCAATCAGGTTTTCAATATTACTTGTGTCTTGTTACTAGTTTTCATTTTATTTTTAGTGGTATGGTTTTGTTTGGATTATATATTATTTATTGTTCAAATTAAATAAAATCTGTTCATGTGCTGAATATAGAATCATAAAGGAGCTATGATGGAGTTAGGGGAGGGGCTATGTAAATTGAGAAAGATGGAGTTCCCTAATGGACCGTTTCTTAAGTTTGAGAAAGATATTTCAGCAGTTTTGGAATTTATCTACCAGACACAGATAGAGTTAGCTGCATGTGAGTAATTCCAACCTTGAAATGTTATGTATCATATGATACATGCAAGTTGTATCTTATTCCGAATCAAAATTGAACTCAGTAAATACAAGTCATTATGCATAGATATGTGTCATGAAGTTCATGTATCTTACCTGTTACCATTCAAGATTAGATTTAATTCACAAATGTTATATGATCGGTTTTGGAGAAATTACATTTTTTAACTTGAATTTGAGCAGGTAGTGATGATGTTTGTGTTGCAATGGATCAATCAGAAAGATCCTACCAACTTATGGGAGAATATGCTGCAGGGAAGCGCGTATATGATCTAGAACTTGGTCGTGATGCATCAGTGTACATTGTGTTCTGCCCTCAAGGGTCTAATGATGCCACTGAAGCTCCAAGGGCAAAGCTTCCCAGGCTTAATGCTACCTCAGAACATCTTGTAGCATATGAATACAAGTAA

Protein sequence

>MS.gene41873.t1

MCSWISSPIQSGSDDVCVAMDQSERSYQLMGEYAAGKRVYDLELGRDASVYIVFCPQGSNDATEAPRAKLPRLNATSEHLVAYEYK