Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82297.t1 | AFK43236.1 | 98.6 | 138 | 2 | 0 | 94 | 231 | 95 | 232 | 5.80E-72 | 281.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82297.t1 | P82658 | 80.0 | 140 | 28 | 0 | 94 | 233 | 81 | 220 | 7.2e-63 | 242.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82297.t1 | G7IWL6 | 98.6 | 138 | 2 | 0 | 94 | 231 | 96 | 233 | 4.2e-72 | 281.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene82297 | 0.834312 | 3.22E-56 | -1.69E-46 |
| MS.gene049171 | MS.gene82297 | 0.813554 | 2.41E-51 | -1.69E-46 |
| MS.gene049217 | MS.gene82297 | 0.831188 | 1.92E-55 | -1.69E-46 |
| MS.gene049222 | MS.gene82297 | 0.837767 | 4.28E-57 | -1.69E-46 |
| MS.gene049285 | MS.gene82297 | 0.859054 | 5.39E-63 | -1.69E-46 |
| MS.gene049320 | MS.gene82297 | 0.862208 | 5.96E-64 | -1.69E-46 |
| MS.gene049351 | MS.gene82297 | 0.824342 | 8.50E-54 | -1.69E-46 |
| MS.gene049369 | MS.gene82297 | 0.810335 | 1.21E-50 | -1.69E-46 |
| MS.gene049427 | MS.gene82297 | 0.835574 | 1.55E-56 | -1.69E-46 |
| MS.gene049609 | MS.gene82297 | 0.85083 | 1.32E-60 | -1.69E-46 |
| MS.gene049615 | MS.gene82297 | 0.841581 | 4.36E-58 | -1.69E-46 |
| MS.gene049805 | MS.gene82297 | 0.879478 | 1.21E-69 | -1.69E-46 |
| MS.gene050076 | MS.gene82297 | 0.811437 | 7.01E-51 | -1.69E-46 |
| MS.gene05010 | MS.gene82297 | 0.837525 | 4.94E-57 | -1.69E-46 |
| MS.gene050163 | MS.gene82297 | 0.850519 | 1.61E-60 | -1.69E-46 |
| MS.gene050165 | MS.gene82297 | 0.819217 | 1.30E-52 | -1.69E-46 |
| MS.gene050333 | MS.gene82297 | 0.830745 | 2.47E-55 | -1.69E-46 |
| MS.gene050432 | MS.gene82297 | 0.834027 | 3.80E-56 | -1.69E-46 |
| MS.gene050504 | MS.gene82297 | 0.836952 | 6.92E-57 | -1.69E-46 |
| MS.gene050518 | MS.gene82297 | 0.851959 | 6.32E-61 | -1.69E-46 |
| MS.gene050520 | MS.gene82297 | 0.856893 | 2.36E-62 | -1.69E-46 |
| MS.gene050593 | MS.gene82297 | 0.884015 | 2.75E-71 | -1.69E-46 |
| MS.gene050612 | MS.gene82297 | 0.839106 | 1.93E-57 | -1.69E-46 |
| MS.gene050670 | MS.gene82297 | 0.816656 | 4.94E-52 | -1.69E-46 |
| MS.gene050675 | MS.gene82297 | 0.83912 | 1.92E-57 | -1.69E-46 |
| MS.gene050686 | MS.gene82297 | 0.836699 | 8.03E-57 | -1.69E-46 |
| MS.gene050699 | MS.gene82297 | 0.861099 | 1.30E-63 | -1.69E-46 |
| MS.gene050747 | MS.gene82297 | 0.847069 | 1.46E-59 | -1.69E-46 |
| MS.gene050767 | MS.gene82297 | 0.860541 | 1.92E-63 | -1.69E-46 |
| MS.gene050770 | MS.gene82297 | 0.860006 | 2.79E-63 | -1.69E-46 |
| MS.gene050772 | MS.gene82297 | 0.880281 | 6.24E-70 | -1.69E-46 |
| MS.gene050783 | MS.gene82297 | 0.803598 | 3.23E-49 | -1.69E-46 |
| MS.gene050950 | MS.gene82297 | 0.841648 | 4.18E-58 | -1.69E-46 |
| MS.gene050986 | MS.gene82297 | 0.852728 | 3.82E-61 | -1.69E-46 |
| MS.gene05100 | MS.gene82297 | 0.821556 | 3.79E-53 | -1.69E-46 |
| MS.gene05101 | MS.gene82297 | 0.8106 | 1.06E-50 | -1.69E-46 |
| MS.gene05105 | MS.gene82297 | 0.815858 | 7.45E-52 | -1.69E-46 |
| MS.gene051167 | MS.gene82297 | -0.818709 | 1.70E-52 | -1.69E-46 |
| MS.gene051228 | MS.gene82297 | 0.822854 | 1.90E-53 | -1.69E-46 |
| MS.gene051255 | MS.gene82297 | 0.806666 | 7.36E-50 | -1.69E-46 |
| MS.gene051387 | MS.gene82297 | 0.889342 | 2.64E-73 | -1.69E-46 |
| MS.gene051509 | MS.gene82297 | 0.800187 | 1.62E-48 | -1.69E-46 |
| MS.gene051551 | MS.gene82297 | 0.817762 | 2.79E-52 | -1.69E-46 |
| MS.gene051666 | MS.gene82297 | 0.844853 | 5.85E-59 | -1.69E-46 |
| MS.gene051692 | MS.gene82297 | 0.816297 | 5.95E-52 | -1.69E-46 |
| MS.gene051694 | MS.gene82297 | 0.835497 | 1.62E-56 | -1.69E-46 |
| MS.gene051810 | MS.gene82297 | 0.818504 | 1.89E-52 | -1.69E-46 |
| MS.gene051858 | MS.gene82297 | 0.816619 | 5.04E-52 | -1.69E-46 |
| MS.gene051877 | MS.gene82297 | 0.803821 | 2.90E-49 | -1.69E-46 |
| MS.gene052271 | MS.gene82297 | 0.874875 | 4.80E-68 | -1.69E-46 |
| MS.gene052310 | MS.gene82297 | 0.803095 | 4.11E-49 | -1.69E-46 |
| MS.gene052322 | MS.gene82297 | 0.809937 | 1.48E-50 | -1.69E-46 |
| MS.gene052326 | MS.gene82297 | 0.800382 | 1.48E-48 | -1.69E-46 |
| MS.gene052379 | MS.gene82297 | 0.822282 | 2.57E-53 | -1.69E-46 |
| MS.gene052421 | MS.gene82297 | 0.863572 | 2.26E-64 | -1.69E-46 |
| MS.gene052484 | MS.gene82297 | 0.801615 | 8.28E-49 | -1.69E-46 |
| MS.gene052485 | MS.gene82297 | 0.805448 | 1.33E-49 | -1.69E-46 |
| MS.gene052503 | MS.gene82297 | 0.800761 | 1.24E-48 | -1.69E-46 |
| MS.gene052504 | MS.gene82297 | 0.817906 | 2.59E-52 | -1.69E-46 |
| MS.gene052506 | MS.gene82297 | 0.821913 | 3.14E-53 | -1.69E-46 |
| MS.gene052510 | MS.gene82297 | 0.811409 | 7.10E-51 | -1.69E-46 |
| MS.gene052615 | MS.gene82297 | 0.861275 | 1.15E-63 | -1.69E-46 |
| MS.gene052822 | MS.gene82297 | 0.824673 | 7.11E-54 | -1.69E-46 |
| MS.gene052916 | MS.gene82297 | 0.831636 | 1.49E-55 | -1.69E-46 |
| MS.gene052966 | MS.gene82297 | 0.803517 | 3.36E-49 | -1.69E-46 |
| MS.gene053009 | MS.gene82297 | 0.816586 | 5.12E-52 | -1.69E-46 |
| MS.gene053103 | MS.gene82297 | 0.812881 | 3.39E-51 | -1.69E-46 |
| MS.gene053104 | MS.gene82297 | 0.826621 | 2.45E-54 | -1.69E-46 |
| MS.gene053107 | MS.gene82297 | 0.822107 | 2.83E-53 | -1.69E-46 |
| MS.gene053203 | MS.gene82297 | 0.82277 | 1.98E-53 | -1.69E-46 |
| MS.gene053207 | MS.gene82297 | 0.874155 | 8.42E-68 | -1.69E-46 |
| MS.gene053213 | MS.gene82297 | 0.894668 | 1.99E-75 | -1.69E-46 |
| MS.gene053286 | MS.gene82297 | 0.8834 | 4.63E-71 | -1.69E-46 |
| MS.gene053322 | MS.gene82297 | 0.869948 | 2.11E-66 | -1.69E-46 |
| MS.gene053436 | MS.gene82297 | 0.861893 | 7.45E-64 | -1.69E-46 |
| MS.gene053449 | MS.gene82297 | 0.802776 | 4.78E-49 | -1.69E-46 |
| MS.gene053574 | MS.gene82297 | 0.843497 | 1.35E-58 | -1.69E-46 |
| MS.gene053579 | MS.gene82297 | 0.809961 | 1.46E-50 | -1.69E-46 |
| MS.gene053626 | MS.gene82297 | 0.813693 | 2.25E-51 | -1.69E-46 |
| MS.gene053632 | MS.gene82297 | 0.844954 | 5.49E-59 | -1.69E-46 |
| MS.gene053813 | MS.gene82297 | 0.856762 | 2.58E-62 | -1.69E-46 |
| MS.gene053824 | MS.gene82297 | 0.802474 | 5.51E-49 | -1.69E-46 |
| MS.gene053916 | MS.gene82297 | 0.844641 | 6.67E-59 | -1.69E-46 |
| MS.gene054376 | MS.gene82297 | 0.858907 | 5.97E-63 | -1.69E-46 |
| MS.gene054408 | MS.gene82297 | 0.811687 | 6.18E-51 | -1.69E-46 |
| MS.gene054595 | MS.gene82297 | 0.850369 | 1.78E-60 | -1.69E-46 |
| MS.gene054600 | MS.gene82297 | 0.844017 | 9.81E-59 | -1.69E-46 |
| MS.gene054642 | MS.gene82297 | 0.865158 | 7.23E-65 | -1.69E-46 |
| MS.gene054802 | MS.gene82297 | 0.871297 | 7.61E-67 | -1.69E-46 |
| MS.gene054803 | MS.gene82297 | 0.865675 | 4.97E-65 | -1.69E-46 |
| MS.gene054804 | MS.gene82297 | 0.873501 | 1.40E-67 | -1.69E-46 |
| MS.gene054927 | MS.gene82297 | 0.809479 | 1.85E-50 | -1.69E-46 |
| MS.gene054954 | MS.gene82297 | 0.824345 | 8.49E-54 | -1.69E-46 |
| MS.gene05505 | MS.gene82297 | 0.86238 | 5.28E-64 | -1.69E-46 |
| MS.gene055206 | MS.gene82297 | 0.835116 | 2.02E-56 | -1.69E-46 |
| MS.gene055224 | MS.gene82297 | 0.817466 | 3.25E-52 | -1.69E-46 |
| MS.gene055277 | MS.gene82297 | 0.812215 | 4.74E-51 | -1.69E-46 |
| MS.gene055289 | MS.gene82297 | 0.803043 | 4.21E-49 | -1.69E-46 |
| MS.gene055362 | MS.gene82297 | 0.856636 | 2.81E-62 | -1.69E-46 |
| MS.gene055397 | MS.gene82297 | 0.846024 | 2.82E-59 | -1.69E-46 |
| MS.gene055412 | MS.gene82297 | 0.820746 | 5.83E-53 | -1.69E-46 |
| MS.gene055499 | MS.gene82297 | 0.862634 | 4.41E-64 | -1.69E-46 |
| MS.gene055502 | MS.gene82297 | 0.835197 | 1.93E-56 | -1.69E-46 |
| MS.gene055577 | MS.gene82297 | 0.829948 | 3.87E-55 | -1.69E-46 |
| MS.gene055636 | MS.gene82297 | 0.833361 | 5.57E-56 | -1.69E-46 |
| MS.gene055638 | MS.gene82297 | 0.818383 | 2.02E-52 | -1.69E-46 |
| MS.gene055647 | MS.gene82297 | 0.880617 | 4.74E-70 | -1.69E-46 |
| MS.gene055722 | MS.gene82297 | 0.852629 | 4.07E-61 | -1.69E-46 |
| MS.gene05592 | MS.gene82297 | 0.893812 | 4.43E-75 | -1.69E-46 |
| MS.gene055941 | MS.gene82297 | 0.856497 | 3.09E-62 | -1.69E-46 |
| MS.gene056083 | MS.gene82297 | 0.827046 | 1.94E-54 | -1.69E-46 |
| MS.gene056374 | MS.gene82297 | 0.882187 | 1.29E-70 | -1.69E-46 |
| MS.gene056407 | MS.gene82297 | 0.838425 | 2.90E-57 | -1.69E-46 |
| MS.gene056410 | MS.gene82297 | 0.801761 | 7.73E-49 | -1.69E-46 |
| MS.gene056422 | MS.gene82297 | 0.804446 | 2.15E-49 | -1.69E-46 |
| MS.gene056510 | MS.gene82297 | 0.816356 | 5.77E-52 | -1.69E-46 |
| MS.gene056511 | MS.gene82297 | 0.804793 | 1.82E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene82297 | 0.862608 | 4.49E-64 | -1.69E-46 |
| MS.gene05666 | MS.gene82297 | 0.803613 | 3.21E-49 | -1.69E-46 |
| MS.gene057011 | MS.gene82297 | 0.864147 | 1.50E-64 | -1.69E-46 |
| MS.gene057076 | MS.gene82297 | 0.808579 | 2.89E-50 | -1.69E-46 |
| MS.gene057086 | MS.gene82297 | 0.806516 | 7.92E-50 | -1.69E-46 |
| MS.gene057124 | MS.gene82297 | 0.816272 | 6.02E-52 | -1.69E-46 |
| MS.gene057129 | MS.gene82297 | 0.87607 | 1.87E-68 | -1.69E-46 |
| MS.gene057146 | MS.gene82297 | 0.834732 | 2.53E-56 | -1.69E-46 |
| MS.gene057285 | MS.gene82297 | 0.819573 | 1.08E-52 | -1.69E-46 |
| MS.gene057331 | MS.gene82297 | 0.881364 | 2.55E-70 | -1.69E-46 |
| MS.gene05735 | MS.gene82297 | 0.828089 | 1.09E-54 | -1.69E-46 |
| MS.gene057416 | MS.gene82297 | 0.90782 | 3.35E-81 | -1.69E-46 |
| MS.gene057926 | MS.gene82297 | 0.813921 | 2.00E-51 | -1.69E-46 |
| MS.gene058006 | MS.gene82297 | 0.823537 | 1.31E-53 | -1.69E-46 |
| MS.gene058019 | MS.gene82297 | 0.878674 | 2.32E-69 | -1.69E-46 |
| MS.gene058293 | MS.gene82297 | 0.856157 | 3.89E-62 | -1.69E-46 |
| MS.gene058323 | MS.gene82297 | 0.884619 | 1.64E-71 | -1.69E-46 |
| MS.gene05837 | MS.gene82297 | 0.822778 | 1.97E-53 | -1.69E-46 |
| MS.gene058419 | MS.gene82297 | 0.863894 | 1.80E-64 | -1.69E-46 |
| MS.gene058544 | MS.gene82297 | 0.830368 | 3.05E-55 | -1.69E-46 |
| MS.gene058660 | MS.gene82297 | 0.831177 | 1.94E-55 | -1.69E-46 |
| MS.gene058773 | MS.gene82297 | 0.825798 | 3.85E-54 | -1.69E-46 |
| MS.gene058838 | MS.gene82297 | 0.805969 | 1.03E-49 | -1.69E-46 |
| MS.gene05883 | MS.gene82297 | 0.834326 | 3.20E-56 | -1.69E-46 |
| MS.gene058903 | MS.gene82297 | 0.829094 | 6.24E-55 | -1.69E-46 |
| MS.gene058993 | MS.gene82297 | 0.82406 | 9.90E-54 | -1.69E-46 |
| MS.gene059022 | MS.gene82297 | 0.829469 | 5.06E-55 | -1.69E-46 |
| MS.gene059023 | MS.gene82297 | 0.809451 | 1.88E-50 | -1.69E-46 |
| MS.gene059029 | MS.gene82297 | 0.817809 | 2.72E-52 | -1.69E-46 |
| MS.gene05934 | MS.gene82297 | 0.81995 | 8.87E-53 | -1.69E-46 |
| MS.gene059367 | MS.gene82297 | 0.830646 | 2.61E-55 | -1.69E-46 |
| MS.gene059368 | MS.gene82297 | 0.823556 | 1.30E-53 | -1.69E-46 |
| MS.gene059536 | MS.gene82297 | 0.832547 | 8.88E-56 | -1.69E-46 |
| MS.gene059676 | MS.gene82297 | 0.823832 | 1.12E-53 | -1.69E-46 |
| MS.gene059677 | MS.gene82297 | 0.838571 | 2.66E-57 | -1.69E-46 |
| MS.gene059688 | MS.gene82297 | 0.806347 | 8.60E-50 | -1.69E-46 |
| MS.gene059711 | MS.gene82297 | 0.827351 | 1.64E-54 | -1.69E-46 |
| MS.gene059718 | MS.gene82297 | 0.856944 | 2.28E-62 | -1.69E-46 |
| MS.gene059756 | MS.gene82297 | 0.822454 | 2.35E-53 | -1.69E-46 |
| MS.gene059801 | MS.gene82297 | 0.806261 | 8.96E-50 | -1.69E-46 |
| MS.gene059902 | MS.gene82297 | 0.815366 | 9.59E-52 | -1.69E-46 |
| MS.gene059960 | MS.gene82297 | 0.875193 | 3.74E-68 | -1.69E-46 |
| MS.gene060193 | MS.gene82297 | 0.864243 | 1.40E-64 | -1.69E-46 |
| MS.gene060356 | MS.gene82297 | 0.81953 | 1.11E-52 | -1.69E-46 |
| MS.gene060403 | MS.gene82297 | 0.81829 | 2.12E-52 | -1.69E-46 |
| MS.gene060482 | MS.gene82297 | 0.830319 | 3.14E-55 | -1.69E-46 |
| MS.gene06077 | MS.gene82297 | 0.821067 | 4.92E-53 | -1.69E-46 |
| MS.gene060804 | MS.gene82297 | 0.855688 | 5.33E-62 | -1.69E-46 |
| MS.gene060818 | MS.gene82297 | 0.836532 | 8.85E-57 | -1.69E-46 |
| MS.gene060835 | MS.gene82297 | 0.853213 | 2.77E-61 | -1.69E-46 |
| MS.gene060837 | MS.gene82297 | 0.835368 | 1.75E-56 | -1.69E-46 |
| MS.gene06092 | MS.gene82297 | 0.859014 | 5.54E-63 | -1.69E-46 |
| MS.gene061041 | MS.gene82297 | 0.822621 | 2.15E-53 | -1.69E-46 |
| MS.gene061051 | MS.gene82297 | -0.805312 | 1.42E-49 | -1.69E-46 |
| MS.gene061134 | MS.gene82297 | 0.830023 | 3.71E-55 | -1.69E-46 |
| MS.gene061150 | MS.gene82297 | 0.863486 | 2.41E-64 | -1.69E-46 |
| MS.gene061171 | MS.gene82297 | 0.823827 | 1.12E-53 | -1.69E-46 |
| MS.gene061200 | MS.gene82297 | 0.895949 | 5.89E-76 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82297.t1 | MTR_3g028150 | 96.137 | 233 | 7 | 1 | 1 | 231 | 1 | 233 | 5.21e-163 | 453 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82297.t1 | AT3G63540 | 79.545 | 132 | 27 | 0 | 102 | 233 | 3 | 134 | 1.17e-77 | 233 |
Find 67 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATATTTAACTTTCCTTTC+TGG | 0.121727 | 3.4:-22055698 | MS.gene82297:CDS |
| GGTGGCGGAGGAGGAGAAAA+TGG | 0.261123 | 3.4:+22056072 | None:intergenic |
| AAACGTTCCTTCCAACCTTC+TGG | 0.272048 | 3.4:+22055794 | None:intergenic |
| AATATTTAACTTTCCTTTCT+GGG | 0.289515 | 3.4:-22055697 | MS.gene82297:CDS |
| TTAATATCAAAAGTTGAAAA+AGG | 0.291067 | 3.4:-22055773 | MS.gene82297:CDS |
| AAAGATTTCAATGTGGGAAT+TGG | 0.309517 | 3.4:-22055105 | MS.gene82297:CDS |
| GGTGGTTGTTGTGGTGGTGC+TGG | 0.347336 | 3.4:+22056051 | None:intergenic |
| TATAGCTGCTGCTGCTGTTA+AGG | 0.390254 | 3.4:+22055967 | None:intergenic |
| GGTGGTTGTGGTGGTTGTTG+TGG | 0.401207 | 3.4:+22056042 | None:intergenic |
| GGTGGTGCTGGTGGCGGAGG+AGG | 0.407598 | 3.4:+22056063 | None:intergenic |
| CCACATTGAAATCTTTAAAC+CGG | 0.413073 | 3.4:+22055142 | None:intergenic |
| GCTGTTAAGGTGGTGGTTAG+AGG | 0.417250 | 3.4:+22055980 | None:intergenic |
| TGTTGTGGTGGTGCTGGTGG+CGG | 0.427977 | 3.4:+22056057 | None:intergenic |
| TTGTTCAAAACAGCATCTTT+TGG | 0.430262 | 3.4:+22055659 | None:intergenic |
| TGTGGTGGTGCTGGTGGCGG+AGG | 0.431777 | 3.4:+22056060 | None:intergenic |
| TTATTTGTCTCACATTGCTC+AGG | 0.435723 | 3.4:-22055018 | MS.gene82297:CDS |
| TTTCAATGTGGGAATTGAAC+GGG | 0.448126 | 3.4:-22055130 | MS.gene82297:CDS |
| TATTATGTTTATGAGATTGA+TGG | 0.453881 | 3.4:-22055545 | MS.gene82297:CDS |
| GGTTGTGGTGGTTGTTGTGG+TGG | 0.455647 | 3.4:+22056045 | None:intergenic |
| GTGAGGGTGGATGTTGAAGT+TGG | 0.468165 | 3.4:-22055063 | MS.gene82297:CDS |
| TGTACCGTAGTAGAGATTGT+AGG | 0.471430 | 3.4:+22055895 | None:intergenic |
| CAATTTCAATATCAACAGTC+GGG | 0.476751 | 3.4:+22054992 | None:intergenic |
| GGGAGATGATTCTGCTGCTA+TGG | 0.482398 | 3.4:+22055916 | None:intergenic |
| GAAGAAGTGAAAGATGATAA+AGG | 0.482410 | 3.4:-22055575 | MS.gene82297:CDS |
| TCTTTATTCCACTCAGGAGT+AGG | 0.485770 | 3.4:+22055452 | None:intergenic |
| GTGGCGGAGGAGGAGAAAAT+GGG | 0.492073 | 3.4:+22056073 | None:intergenic |
| GCAGCAAACTACGGCGGCTA+CGG | 0.501712 | 3.4:-22055863 | MS.gene82297:CDS |
| TGATATGGTGGTGGTTGTGG+TGG | 0.513282 | 3.4:+22056033 | None:intergenic |
| AATTTGCAAGATATTATTAG+TGG | 0.515902 | 3.4:-22055617 | MS.gene82297:CDS |
| TGCTGCTGCTGTTAAGGTGG+TGG | 0.518372 | 3.4:+22055973 | None:intergenic |
| TCCTTATCTTTATTCCACTC+AGG | 0.519893 | 3.4:+22055446 | None:intergenic |
| TTGTGTTTGAATGAGATAAG+AGG | 0.520933 | 3.4:+22056007 | None:intergenic |
| GCGGAGGAGGAGAAAATGGG+TGG | 0.522990 | 3.4:+22056076 | None:intergenic |
| CCTCCCGCTTAAAACTCATT+CGG | 0.523545 | 3.4:-22054950 | MS.gene82297:CDS |
| TCCTGAGTGGAATAAAGATA+AGG | 0.526998 | 3.4:-22055447 | MS.gene82297:CDS |
| CGGTTTAAAGATTTCAATGT+GGG | 0.529670 | 3.4:-22055141 | MS.gene82297:CDS |
| TCATGCCCACGCTAGGCGTG+AGG | 0.533836 | 3.4:-22055080 | MS.gene82297:CDS |
| ATTGGACTCATGCCCACGCT+AGG | 0.540309 | 3.4:-22055087 | MS.gene82297:CDS |
| ATCCACCCTCACGCCTAGCG+TGG | 0.542692 | 3.4:+22055074 | None:intergenic |
| GGTTGTTGTGGTGGTGCTGG+TGG | 0.546258 | 3.4:+22056054 | None:intergenic |
| GTACCGTAGTAGAGATTGTA+GGG | 0.551840 | 3.4:+22055896 | None:intergenic |
| ACAATTTCAATATCAACAGT+CGG | 0.553544 | 3.4:+22054991 | None:intergenic |
| AGGTGGTGATGTTGTCAGAA+TGG | 0.556486 | 3.4:+22055940 | None:intergenic |
| ATTTCAATGTGGGAATTGAA+CGG | 0.557096 | 3.4:-22055131 | MS.gene82297:CDS |
| GCCCACGCTAGGCGTGAGGG+TGG | 0.558340 | 3.4:-22055076 | MS.gene82297:CDS |
| TCTACGACGTTCCAGAAGGT+TGG | 0.565732 | 3.4:-22055805 | MS.gene82297:CDS |
| CGGGCTAAAGATTTCAATGT+GGG | 0.568974 | 3.4:-22055111 | MS.gene82297:CDS |
| AGCTGCTGCTGCTGTTAAGG+TGG | 0.570466 | 3.4:+22055970 | None:intergenic |
| GCAAACTACGGCGGCTACGG+CGG | 0.573351 | 3.4:-22055860 | MS.gene82297:CDS |
| CATGCCCACGCTAGGCGTGA+GGG | 0.574666 | 3.4:-22055079 | MS.gene82297:CDS |
| TGTTGATATTGAAATTGTGA+TGG | 0.579542 | 3.4:-22054986 | MS.gene82297:CDS |
| GAGGAGGAGAAAATGGGTGG+TGG | 0.579804 | 3.4:+22056079 | None:intergenic |
| GATGATTCTGCTGCTATGGA+AGG | 0.583786 | 3.4:+22055920 | None:intergenic |
| CGACGTTCCAGAAGGTTGGA+AGG | 0.585452 | 3.4:-22055801 | MS.gene82297:CDS |
| GCAGCAAGTGCAGCAAACTA+CGG | 0.594999 | 3.4:-22055872 | MS.gene82297:CDS |
| TCCACCCTCACGCCTAGCGT+GGG | 0.600370 | 3.4:+22055075 | None:intergenic |
| TCTCCCTACAATCTCTACTA+CGG | 0.607493 | 3.4:-22055899 | MS.gene82297:CDS |
| GCAAGTGCAGCAAACTACGG+CGG | 0.619443 | 3.4:-22055869 | MS.gene82297:CDS |
| TACATCTACGACGTTCCAGA+AGG | 0.628307 | 3.4:-22055809 | MS.gene82297:CDS |
| CTAACTGACGAAACCCAGAA+AGG | 0.634618 | 3.4:+22055684 | None:intergenic |
| TTAATGCTCCTACTCCTGAG+TGG | 0.644053 | 3.4:-22055460 | MS.gene82297:CDS |
| GATTCTGCTGCTATGGAAGG+TGG | 0.667537 | 3.4:+22055923 | None:intergenic |
| CCGGTTTAAAGATTTCAATG+TGG | 0.678539 | 3.4:-22055142 | MS.gene82297:CDS |
| TGGTGATGATGAGAAAATTG+TGG | 0.687506 | 3.4:+22056099 | None:intergenic |
| AATTTCAATATCAACAGTCG+GGG | 0.689162 | 3.4:+22054993 | None:intergenic |
| ACGGGCTAAAGATTTCAATG+TGG | 0.712707 | 3.4:-22055112 | MS.gene82297:CDS |
| TGGTGATGTTGTCAGAATGG+TGG | 0.721157 | 3.4:+22055943 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATAAATAATAAATAATA+TGG | - | chr3.4:22055680-22055699 | MS.gene82297:CDS | 0.0% |
| !! | TTAATATCAAAAGTTGAAAA+AGG | - | chr3.4:22055263-22055282 | MS.gene82297:intron | 15.0% |
| !! | AATTTGCAAGATATTATTAG+TGG | - | chr3.4:22055419-22055438 | MS.gene82297:intron | 20.0% |
| !! | TATTATGTTTATGAGATTGA+TGG | - | chr3.4:22055491-22055510 | MS.gene82297:CDS | 20.0% |
| !!! | AATATGGTTTTGTTTAACAA+TGG | - | chr3.4:22055696-22055715 | MS.gene82297:CDS | 20.0% |
| !!! | AATATTTAACTTTCCTTTCT+GGG | - | chr3.4:22055339-22055358 | MS.gene82297:intron | 20.0% |
| !!! | ATATTCTATGATAATGAGAA+TGG | - | chr3.4:22055719-22055738 | MS.gene82297:CDS | 20.0% |
| ! | ACAATTTCAATATCAACAGT+CGG | + | chr3.4:22056048-22056067 | None:intergenic | 25.0% |
| ! | TGTTGATATTGAAATTGTGA+TGG | - | chr3.4:22056050-22056069 | MS.gene82297:CDS | 25.0% |
| !! | AAAGTTGAAAAAGGTACAAA+TGG | - | chr3.4:22055272-22055291 | MS.gene82297:intron | 25.0% |
| !! | GAATATTTAACTTTCCTTTC+TGG | - | chr3.4:22055338-22055357 | MS.gene82297:intron | 25.0% |
| AAAGATTTCAATGTGGGAAT+TGG | - | chr3.4:22055901-22055920 | MS.gene82297:CDS | 30.0% | |
| AATTTCAATATCAACAGTCG+GGG | + | chr3.4:22056046-22056065 | None:intergenic | 30.0% | |
| ATTTAACATGATATCAGAGC+CGG | - | chr3.4:22055875-22055894 | MS.gene82297:CDS | 30.0% | |
| ATTTCAATGTGGGAATTGAA+CGG | - | chr3.4:22055905-22055924 | MS.gene82297:CDS | 30.0% | |
| CAATTTCAATATCAACAGTC+GGG | + | chr3.4:22056047-22056066 | None:intergenic | 30.0% | |
| CCACATTGAAATCTTTAAAC+CGG | + | chr3.4:22055897-22055916 | None:intergenic | 30.0% | |
| CGGTTTAAAGATTTCAATGT+GGG | - | chr3.4:22055895-22055914 | MS.gene82297:CDS | 30.0% | |
| GAAGAAGTGAAAGATGATAA+AGG | - | chr3.4:22055461-22055480 | MS.gene82297:CDS | 30.0% | |
| TATCTCAAAAGCTAACTCAA+AGG | + | chr3.4:22055843-22055862 | None:intergenic | 30.0% | |
| TTGTGTTTGAATGAGATAAG+AGG | + | chr3.4:22055032-22055051 | None:intergenic | 30.0% | |
| ! | TCTTTCTCTGTTCTTTTCTT+TGG | + | chr3.4:22055320-22055339 | None:intergenic | 30.0% |
| ! | TTGTTCAAAACAGCATCTTT+TGG | + | chr3.4:22055380-22055399 | None:intergenic | 30.0% |
| !! | TGAGATAAGAGGTTTTGATA+TGG | + | chr3.4:22055021-22055040 | None:intergenic | 30.0% |
| ATTTATACACTCAACAGCCT+GGG | + | chr3.4:22055802-22055821 | None:intergenic | 35.0% | |
| CCGGTTTAAAGATTTCAATG+TGG | - | chr3.4:22055894-22055913 | MS.gene82297:CDS | 35.0% | |
| TATTTATACACTCAACAGCC+TGG | + | chr3.4:22055803-22055822 | None:intergenic | 35.0% | |
| TCCTGAGTGGAATAAAGATA+AGG | - | chr3.4:22055589-22055608 | MS.gene82297:CDS | 35.0% | |
| TCCTTATCTTTATTCCACTC+AGG | + | chr3.4:22055593-22055612 | None:intergenic | 35.0% | |
| TTATTTGCCTCATATTGCTC+AGG | - | chr3.4:22055774-22055793 | MS.gene82297:CDS | 35.0% | |
| TTATTTGTCTCACATTGCTC+AGG | - | chr3.4:22056018-22056037 | MS.gene82297:CDS | 35.0% | |
| TTTCAATGTGGGAATTGAAC+GGG | - | chr3.4:22055906-22055925 | MS.gene82297:CDS | 35.0% | |
| ! | TGGTGATGATGAGAAAATTG+TGG | + | chr3.4:22054940-22054959 | None:intergenic | 35.0% |
| !! | GATAAGAGGTTTTGATATGG+TGG | + | chr3.4:22055018-22055037 | None:intergenic | 35.0% |
| !!! | AATGCCGAATGAGTTTTAAG+CGG | + | chr3.4:22056093-22056112 | None:intergenic | 35.0% |
| CGGGCTAAAGATTTCAATGT+GGG | - | chr3.4:22055925-22055944 | MS.gene82297:CDS | 40.0% | |
| GTACCGTAGTAGAGATTGTA+GGG | + | chr3.4:22055143-22055162 | None:intergenic | 40.0% | |
| TCTCCCTACAATCTCTACTA+CGG | - | chr3.4:22055137-22055156 | MS.gene82297:CDS | 40.0% | |
| TCTTTATTCCACTCAGGAGT+AGG | + | chr3.4:22055587-22055606 | None:intergenic | 40.0% | |
| TGTACCGTAGTAGAGATTGT+AGG | + | chr3.4:22055144-22055163 | None:intergenic | 40.0% | |
| ! | ACGGGCTAAAGATTTCAATG+TGG | - | chr3.4:22055924-22055943 | MS.gene82297:CDS | 40.0% |
| !! | AAGAGGTTTTGATATGGTGG+TGG | + | chr3.4:22055015-22055034 | None:intergenic | 40.0% |
| !!! | ATGCCGAATGAGTTTTAAGC+GGG | + | chr3.4:22056092-22056111 | None:intergenic | 40.0% |
| !!! | TTTTGATATGGTGGTGGTTG+TGG | + | chr3.4:22055009-22055028 | None:intergenic | 40.0% |
| AAACGTTCCTTCCAACCTTC+TGG | + | chr3.4:22055245-22055264 | None:intergenic | 45.0% | |
| CCTCCCGCTTAAAACTCATT+CGG | - | chr3.4:22056086-22056105 | MS.gene82297:CDS | 45.0% | |
| CTAACTGACGAAACCCAGAA+AGG | + | chr3.4:22055355-22055374 | None:intergenic | 45.0% | |
| GATGATTCTGCTGCTATGGA+AGG | + | chr3.4:22055119-22055138 | None:intergenic | 45.0% | |
| TACATCTACGACGTTCCAGA+AGG | - | chr3.4:22055227-22055246 | MS.gene82297:intron | 45.0% | |
| TATAGCTGCTGCTGCTGTTA+AGG | + | chr3.4:22055072-22055091 | None:intergenic | 45.0% | |
| TTAATGCTCCTACTCCTGAG+TGG | - | chr3.4:22055576-22055595 | MS.gene82297:CDS | 45.0% | |
| ! | AGGTGGTGATGTTGTCAGAA+TGG | + | chr3.4:22055099-22055118 | None:intergenic | 45.0% |
| ! | TGGTGATGTTGTCAGAATGG+TGG | + | chr3.4:22055096-22055115 | None:intergenic | 45.0% |
| !!! | AGTTTTAAGCGGGAGGTCTA+GGG | + | chr3.4:22056082-22056101 | None:intergenic | 45.0% |
| CTCATATTGCTCAGGTTCCC+AGG | - | chr3.4:22055782-22055801 | MS.gene82297:CDS | 50.0% | |
| CTGGGAACCTGAGCAATATG+AGG | + | chr3.4:22055784-22055803 | None:intergenic | 50.0% | |
| GATTCTGCTGCTATGGAAGG+TGG | + | chr3.4:22055116-22055135 | None:intergenic | 50.0% | |
| GCAGCAAGTGCAGCAAACTA+CGG | - | chr3.4:22055164-22055183 | MS.gene82297:intron | 50.0% | |
| GGGAGATGATTCTGCTGCTA+TGG | + | chr3.4:22055123-22055142 | None:intergenic | 50.0% | |
| GTGAGGGTGGATGTTGAAGT+TGG | - | chr3.4:22055973-22055992 | MS.gene82297:CDS | 50.0% | |
| TCTACGACGTTCCAGAAGGT+TGG | - | chr3.4:22055231-22055250 | MS.gene82297:intron | 50.0% | |
| ! | GCTGTTAAGGTGGTGGTTAG+AGG | + | chr3.4:22055059-22055078 | None:intergenic | 50.0% |
| !! | TGATATGGTGGTGGTTGTGG+TGG | + | chr3.4:22055006-22055025 | None:intergenic | 50.0% |
| !!! | CCGAATGAGTTTTAAGCGGG+AGG | + | chr3.4:22056089-22056108 | None:intergenic | 50.0% |
| !!! | GAGTTTTAAGCGGGAGGTCT+AGG | + | chr3.4:22056083-22056102 | None:intergenic | 50.0% |
| AGCTGCTGCTGCTGTTAAGG+TGG | + | chr3.4:22055069-22055088 | None:intergenic | 55.0% | |
| ATTGGACTCATGCCCACGCT+AGG | - | chr3.4:22055949-22055968 | MS.gene82297:CDS | 55.0% | |
| CGACGTTCCAGAAGGTTGGA+AGG | - | chr3.4:22055235-22055254 | MS.gene82297:intron | 55.0% | |
| GAGGAGGAGAAAATGGGTGG+TGG | + | chr3.4:22054960-22054979 | None:intergenic | 55.0% | |
| GCAAGTGCAGCAAACTACGG+CGG | - | chr3.4:22055167-22055186 | MS.gene82297:intron | 55.0% | |
| GGTGGTTGTGGTGGTTGTTG+TGG | + | chr3.4:22054997-22055016 | None:intergenic | 55.0% | |
| GGTTGTGGTGGTTGTTGTGG+TGG | + | chr3.4:22054994-22055013 | None:intergenic | 55.0% | |
| TGCTGCTGCTGTTAAGGTGG+TGG | + | chr3.4:22055066-22055085 | None:intergenic | 55.0% | |
| ! | GTGGCGGAGGAGGAGAAAAT+GGG | + | chr3.4:22054966-22054985 | None:intergenic | 55.0% |
| GCAGCAAACTACGGCGGCTA+CGG | - | chr3.4:22055173-22055192 | MS.gene82297:intron | 60.0% | |
| GCGGAGGAGGAGAAAATGGG+TGG | + | chr3.4:22054963-22054982 | None:intergenic | 60.0% | |
| ! | GGTGGTTGTTGTGGTGGTGC+TGG | + | chr3.4:22054988-22055007 | None:intergenic | 60.0% |
| ! | GGTTGTTGTGGTGGTGCTGG+TGG | + | chr3.4:22054985-22055004 | None:intergenic | 60.0% |
| !! | GGTGGCGGAGGAGGAGAAAA+TGG | + | chr3.4:22054967-22054986 | None:intergenic | 60.0% |
| !! | TGTTGTGGTGGTGCTGGTGG+CGG | + | chr3.4:22054982-22055001 | None:intergenic | 60.0% |
| ATCCACCCTCACGCCTAGCG+TGG | + | chr3.4:22055965-22055984 | None:intergenic | 65.0% | |
| CATGCCCACGCTAGGCGTGA+GGG | - | chr3.4:22055957-22055976 | MS.gene82297:CDS | 65.0% | |
| GCAAACTACGGCGGCTACGG+CGG | - | chr3.4:22055176-22055195 | MS.gene82297:intron | 65.0% | |
| TCATGCCCACGCTAGGCGTG+AGG | - | chr3.4:22055956-22055975 | MS.gene82297:CDS | 65.0% | |
| TCCACCCTCACGCCTAGCGT+GGG | + | chr3.4:22055964-22055983 | None:intergenic | 65.0% | |
| !! | TGTGGTGGTGCTGGTGGCGG+AGG | + | chr3.4:22054979-22054998 | None:intergenic | 70.0% |
| GCCCACGCTAGGCGTGAGGG+TGG | - | chr3.4:22055960-22055979 | MS.gene82297:CDS | 75.0% | |
| !! | GGTGGTGCTGGTGGCGGAGG+AGG | + | chr3.4:22054976-22054995 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 22054933 | 22056125 | 22054933 | ID=MS.gene82297 |
| chr3.4 | mRNA | 22054933 | 22056125 | 22054933 | ID=MS.gene82297.t1;Parent=MS.gene82297 |
| chr3.4 | exon | 22055433 | 22056125 | 22055433 | ID=MS.gene82297.t1.exon1;Parent=MS.gene82297.t1 |
| chr3.4 | CDS | 22055433 | 22056125 | 22055433 | ID=cds.MS.gene82297.t1;Parent=MS.gene82297.t1 |
| chr3.4 | exon | 22054933 | 22055166 | 22054933 | ID=MS.gene82297.t1.exon2;Parent=MS.gene82297.t1 |
| chr3.4 | CDS | 22054933 | 22055166 | 22054933 | ID=cds.MS.gene82297.t1;Parent=MS.gene82297.t1 |
| Gene Sequence |
| Protein sequence |
