AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92746


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAGACTAATTAATTCTT+TGG 0.209215 3.1:-92940527 None:intergenic
ATGATCAAAGGTGCTCTTCC+TGG 0.365904 3.1:+92940449 MS.gene92746:intron
CTCTTGGAACCATGGCTCAT+TGG 0.403785 3.1:-92940280 None:intergenic
TCCAAGTTGTCTGTGACTCT+TGG 0.442433 3.1:-92940296 None:intergenic
TCCAAGAGTCACAGACAACT+TGG 0.460086 3.1:+92940295 MS.gene92746:CDS
TTCTTGCCAACAATCTTAGC+GGG 0.460342 3.1:-92940500 None:intergenic
TACACCTGGTCGCGTATACA+AGG 0.469173 3.1:+92940336 MS.gene92746:CDS
CACAGACAACTTGGATCCAT+TGG 0.481197 3.1:+92940304 MS.gene92746:CDS
TACGCGACCAGGTGTAGTTC+CGG 0.494636 3.1:-92940329 None:intergenic
TTTCCCTTGTATACGCGACC+AGG 0.499382 3.1:-92940340 None:intergenic
AGAAGATTACCAGGCTTACC+AGG 0.500896 3.1:-92940467 None:intergenic
GGTGCTCTTCCTGGTAAGCC+TGG 0.514459 3.1:+92940458 MS.gene92746:intron
AAGCGTCATAACTTCAAAAG+AGG 0.517261 3.1:+92940256 MS.gene92746:CDS
AGGTGTAGTTCCGGCACCAA+TGG 0.518535 3.1:-92940320 None:intergenic
GTAAGCCTGGTAATCTTCTA+CGG 0.530779 3.1:+92940471 MS.gene92746:CDS
TACAAGGGAAAGAAGATGCC+TGG 0.536322 3.1:+92940352 MS.gene92746:CDS
GTCTGTGACTCTTGGAACCA+TGG 0.543733 3.1:-92940288 None:intergenic
CAACTTGGATCCATTGGTGC+CGG 0.569215 3.1:+92940310 MS.gene92746:CDS
ATTGGTGCCGGAACTACACC+TGG 0.580799 3.1:+92940322 MS.gene92746:CDS
GTTCTTGCCAACAATCTTAG+CGG 0.594511 3.1:-92940501 None:intergenic
GCTATCCGTAGAAGATTACC+AGG 0.602366 3.1:-92940476 None:intergenic
TCTTGCCAACAATCTTAGCG+GGG 0.638407 3.1:-92940499 None:intergenic
AAAAGAGGACCAATGAGCCA+TGG 0.640937 3.1:+92940271 MS.gene92746:CDS
CTTGCCAACAATCTTAGCGG+GGG 0.643846 3.1:-92940498 None:intergenic
ACACCTGGTCGCGTATACAA+GGG 0.687415 3.1:+92940337 MS.gene92746:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTTAATGTTATCATGATCAA+AGG + chr3.1:92940437-92940456 MS.gene92746:intron 25.0%
TTAACAATCTTAAGCTTTCC+TGG - chr3.1:92940404-92940423 None:intergenic 30.0%
AAGCGTCATAACTTCAAAAG+AGG + chr3.1:92940256-92940275 MS.gene92746:CDS 35.0%
GTAAGCCTGGTAATCTTCTA+CGG + chr3.1:92940471-92940490 MS.gene92746:CDS 40.0%
GTTCTTGCCAACAATCTTAG+CGG - chr3.1:92940504-92940523 None:intergenic 40.0%
TTCTTGCCAACAATCTTAGC+GGG - chr3.1:92940503-92940522 None:intergenic 40.0%
AAAAGAGGACCAATGAGCCA+TGG + chr3.1:92940271-92940290 MS.gene92746:CDS 45.0%
AAAGAAGATGCCTGGTAGGA+TGG + chr3.1:92940360-92940379 MS.gene92746:intron 45.0%
AAGAAGATGCCTGGTAGGAT+GGG + chr3.1:92940361-92940380 MS.gene92746:intron 45.0%
AGAAGATTACCAGGCTTACC+AGG - chr3.1:92940470-92940489 None:intergenic 45.0%
CACAGACAACTTGGATCCAT+TGG + chr3.1:92940304-92940323 MS.gene92746:CDS 45.0%
GCTATCCGTAGAAGATTACC+AGG - chr3.1:92940479-92940498 None:intergenic 45.0%
TACAAGGGAAAGAAGATGCC+TGG + chr3.1:92940352-92940371 MS.gene92746:CDS 45.0%
TCCAAGAGTCACAGACAACT+TGG + chr3.1:92940295-92940314 MS.gene92746:CDS 45.0%
TCCAAGTTGTCTGTGACTCT+TGG - chr3.1:92940299-92940318 None:intergenic 45.0%
TCTTGCCAACAATCTTAGCG+GGG - chr3.1:92940502-92940521 None:intergenic 45.0%
!! ATGATCAAAGGTGCTCTTCC+TGG + chr3.1:92940449-92940468 MS.gene92746:intron 45.0%
ACACCTGGTCGCGTATACAA+GGG + chr3.1:92940337-92940356 MS.gene92746:CDS 50.0%
AGGGAAAGAAGATGCCTGGT+AGG + chr3.1:92940356-92940375 MS.gene92746:intron 50.0%
ATAGCCCCCGCTAAGATTGT+TGG + chr3.1:92940494-92940513 MS.gene92746:CDS 50.0%
CAACTTGGATCCATTGGTGC+CGG + chr3.1:92940310-92940329 MS.gene92746:CDS 50.0%
CTCTTGGAACCATGGCTCAT+TGG - chr3.1:92940283-92940302 None:intergenic 50.0%
CTTGCCAACAATCTTAGCGG+GGG - chr3.1:92940501-92940520 None:intergenic 50.0%
GTCTGTGACTCTTGGAACCA+TGG - chr3.1:92940291-92940310 None:intergenic 50.0%
TACACCTGGTCGCGTATACA+AGG + chr3.1:92940336-92940355 MS.gene92746:CDS 50.0%
TTTCCCTTGTATACGCGACC+AGG - chr3.1:92940343-92940362 None:intergenic 50.0%
! AGCTTTCCTGGTCTTCCTGT+TGG - chr3.1:92940392-92940411 None:intergenic 50.0%
AAGATGCCTGGTAGGATGGG+TGG + chr3.1:92940364-92940383 MS.gene92746:intron 55.0%
AGGTGTAGTTCCGGCACCAA+TGG - chr3.1:92940323-92940342 None:intergenic 55.0%
GTAGGATGGGTGGAACCAAC+AGG + chr3.1:92940374-92940393 MS.gene92746:intron 55.0%
GTGGAACCAACAGGAAGACC+AGG + chr3.1:92940383-92940402 MS.gene92746:intron 55.0%
TACGCGACCAGGTGTAGTTC+CGG - chr3.1:92940332-92940351 None:intergenic 55.0%
TTGGTTCCACCCATCCTACC+AGG - chr3.1:92940373-92940392 None:intergenic 55.0%
!! ATTGGTGCCGGAACTACACC+TGG + chr3.1:92940322-92940341 MS.gene92746:CDS 55.0%
GGTGCTCTTCCTGGTAAGCC+TGG + chr3.1:92940458-92940477 MS.gene92746:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 92940248 92940538 92940248 ID=MS.gene92746
chr3.1 mRNA 92940248 92940538 92940248 ID=MS.gene92746.t1;Parent=MS.gene92746
chr3.1 exon 92940248 92940373 92940248 ID=MS.gene92746.t1.exon1;Parent=MS.gene92746.t1
chr3.1 CDS 92940248 92940373 92940248 ID=cds.MS.gene92746.t1;Parent=MS.gene92746.t1
chr3.1 exon 92940459 92940538 92940459 ID=MS.gene92746.t1.exon2;Parent=MS.gene92746.t1
chr3.1 CDS 92940459 92940538 92940459 ID=cds.MS.gene92746.t1;Parent=MS.gene92746.t1
Gene Sequence

>MS.gene92746

GTGGTATCAAGCGTCATAACTTCAAAAGAGGACCAATGAGCCATGGTTCCAAGAGTCACAGACAACTTGGATCCATTGGTGCCGGAACTACACCTGGTCGCGTATACAAGGGAAAGAAGATGCCTGGTAGGATGGGTGGAACCAACAGGAAGACCAGGAAAGCTTAAGATTGTTAAAATTGATAAAGATCTTAATGTTATCATGATCAAAGGTGCTCTTCCTGGTAAGCCTGGTAATCTTCTACGGATAGCCCCCGCTAAGATTGTTGGCAAGAACATTCCAAAGAATTAA

Protein sequence

>MS.gene92746.t1

VVSSVITSKEDQUAMVPRVTDNLDPLVPELHLVAYTRERRCLVLFLVSLVIFYGUPPLRLLARTFQRI