AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066800


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066800.t1 MTR_3g086050 94.595 222 11 1 1 222 1 221 9.27e-137 382
MS.gene066800.t1 MTR_3g052760 44.206 233 102 9 4 222 17 235 9.13e-56 177
MS.gene066800.t1 MTR_3g092460 41.250 160 74 4 64 222 95 235 1.54e-33 120
MS.gene066800.t1 MTR_3g092460 41.401 157 72 4 67 222 129 266 5.31e-32 117
MS.gene066800.t1 MTR_0004s0510 28.509 228 139 7 1 222 1 210 2.60e-21 88.2
MS.gene066800.t1 MTR_0004s0510 33.121 157 86 5 68 222 70 209 9.84e-21 86.7
MS.gene066800.t1 MTR_4g088445 34.783 92 57 2 124 212 38 129 6.37e-13 64.3
MS.gene066800.t1 MTR_7g102590 40.230 87 46 2 123 208 31 112 1.51e-12 62.8
MS.gene066800.t1 MTR_5g081530 27.941 136 81 4 81 207 7 134 2.64e-12 62.8
MS.gene066800.t1 MTR_4g010320 35.088 114 64 2 119 222 118 231 3.34e-12 63.9
MS.gene066800.t1 MTR_6g022000 36.458 96 55 4 119 208 28 123 1.21e-11 60.8
MS.gene066800.t1 MTR_6g061940 28.037 107 72 2 118 221 53 157 6.31e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066800.t1 AT4G27670 43.182 176 71 7 56 222 72 227 5.19e-38 132
MS.gene066800.t1 AT1G52560 40.952 105 58 3 122 222 121 225 7.27e-20 84.7
MS.gene066800.t1 AT1G52560 40.952 105 58 3 122 222 128 232 8.29e-20 84.7
MS.gene066800.t1 AT4G25200 33.962 159 88 5 51 208 53 195 5.21e-19 82.0
MS.gene066800.t1 AT5G51440 32.203 177 99 5 33 208 39 195 2.25e-18 80.5
MS.gene066800.t1 AT1G07400 31.783 129 72 6 91 209 19 141 1.46e-12 63.5
MS.gene066800.t1 AT5G37670 36.792 106 58 4 125 221 28 133 7.03e-12 61.2
MS.gene066800.t1 AT1G53540 32.673 101 65 2 124 221 55 155 1.94e-11 60.5
MS.gene066800.t1 AT1G54050 34.188 117 62 4 104 206 22 137 4.91e-11 59.3
MS.gene066800.t1 AT1G54050 34.188 117 62 4 104 206 22 137 4.91e-11 59.3
MS.gene066800.t1 AT1G59860 34.951 103 60 4 117 215 46 145 5.32e-11 59.3

Find 63 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTCATCATTCTTCTTCT+TGG 0.080906 3.2:+73958038 None:intergenic
TTCTCAAACTGCACATTCTT+TGG 0.245312 3.2:+73957967 None:intergenic
CTGCTGCACCGATAGGGTTA+TGG 0.285020 3.2:-73958335 MS.gene066800:CDS
AAGGAGTAGGTGGGTATAGA+AGG 0.294040 3.2:-73958197 MS.gene066800:CDS
ACAAAGAGGATGTTAAAGTT+TGG 0.296691 3.2:-73958101 MS.gene066800:CDS
CACTCTTTATCCTTAAATCT+TGG 0.324147 3.2:-73958513 MS.gene066800:CDS
TGTGCAGTTTGAGAATATTA+AGG 0.336710 3.2:-73957959 MS.gene066800:CDS
TGCTGCACCGATAGGGTTAT+GGG 0.365142 3.2:-73958334 MS.gene066800:CDS
CAAAGAGGATGTTAAAGTTT+GGG 0.367241 3.2:-73958100 MS.gene066800:CDS
TTCTCTTGCTTCACCTGTCA+TGG 0.368553 3.2:+73958428 None:intergenic
GAATATTAAGGCTGAGGTTA+AGG 0.368713 3.2:-73957947 MS.gene066800:CDS
AACATTAAGATCAAGGACTT+TGG 0.390389 3.2:+73957879 None:intergenic
CAGGTTAAGACCAAGATTTA+AGG 0.391199 3.2:+73958503 None:intergenic
AGTTTGTCTTGATGAACTCT+TGG 0.419007 3.2:+73958470 None:intergenic
TGATGAACTCTTGGAAATCA+TGG 0.438584 3.2:+73958479 None:intergenic
AAGGACAGTGCAAGAGATGA+TGG 0.443408 3.2:-73958295 MS.gene066800:CDS
GTCATTGAACATTAAGATCA+AGG 0.444311 3.2:+73957872 None:intergenic
TCTCCTTCTTTAATCTCCCA+TGG 0.457000 3.2:+73958162 None:intergenic
ATTAAGGCTGAGGTTAAGGA+TGG 0.462230 3.2:-73957943 MS.gene066800:CDS
TCATCGCCGCTACCGATTGA+AGG 0.462817 3.2:-73958216 MS.gene066800:CDS
CTCTCTTCTTGGGTTCGGAC+TGG 0.469722 3.2:+73958364 None:intergenic
TCAATTGTACTCATTGCAAA+CGG 0.479546 3.2:+73958246 None:intergenic
CATAACCCTATCGGTGCAGC+AGG 0.490168 3.2:+73958336 None:intergenic
GAATAAGTAGTAGTAGCCTT+AGG 0.490783 3.2:+73957901 None:intergenic
CAATTGTACTCATTGCAAAC+GGG 0.496144 3.2:+73958247 None:intergenic
TCAATCGGTAGCGGCGATGA+AGG 0.505690 3.2:+73958219 None:intergenic
CTTCGAGTCAAAGCCATGAC+AGG 0.510747 3.2:-73958441 MS.gene066800:CDS
GTTTGAGAATATTAAGGCTG+AGG 0.515148 3.2:-73957953 MS.gene066800:CDS
AGAATGATGAAGATGAAGAA+TGG 0.516116 3.2:-73958029 MS.gene066800:CDS
TCTTTACATAACCATTCCTA+AGG 0.516609 3.2:-73957917 MS.gene066800:CDS
AGTAGGTGGGTATAGAAGGA+GGG 0.522121 3.2:-73958193 MS.gene066800:CDS
ACATCCTCTTTGTTCATACC+AGG 0.528873 3.2:+73958111 None:intergenic
GAGTAGGTGGGTATAGAAGG+AGG 0.533601 3.2:-73958194 MS.gene066800:CDS
CTGTCATGGCTTTGACTCGA+AGG 0.534564 3.2:+73958442 None:intergenic
TACCCACCTACTCCTTCAAT+CGG 0.537111 3.2:+73958204 None:intergenic
AACTCTTGGAAATCATGGAC+AGG 0.539369 3.2:+73958484 None:intergenic
TAAGGCTGAGGTTAAGGATG+GGG 0.545488 3.2:-73957941 MS.gene066800:CDS
ACACCATGGGAGATTAAAGA+AGG 0.546168 3.2:-73958165 MS.gene066800:CDS
GTAGGTGGGTATAGAAGGAG+GGG 0.548522 3.2:-73958192 MS.gene066800:CDS
GAGAAAGTGAATACAAGATG+AGG 0.559520 3.2:-73958143 MS.gene066800:CDS
GAAGAATGGTCTAAGAGCTA+TGG 0.561468 3.2:-73958015 MS.gene066800:CDS
AAGATGAGGTTTGATATGCC+TGG 0.574216 3.2:-73958129 MS.gene066800:CDS
TTGGGTGGAAGAGAAGATGT+TGG 0.576341 3.2:-73958082 MS.gene066800:CDS
CCGCTACCGATTGAAGGAGT+AGG 0.578817 3.2:-73958210 MS.gene066800:CDS
AGAGGATGTTAAAGTTTGGG+TGG 0.581219 3.2:-73958097 MS.gene066800:CDS
TCTTGCACTGTCCTTGTCGT+AGG 0.581285 3.2:+73958303 None:intergenic
GGGAACAATGGAAAGCATGA+TGG 0.589950 3.2:-73958274 MS.gene066800:CDS
TCTTCTTGGGTTCGGACTGG+CGG 0.591487 3.2:+73958367 None:intergenic
TTAAGGCTGAGGTTAAGGAT+GGG 0.596993 3.2:-73957942 MS.gene066800:CDS
GCAAGAGATGATGGGAACAA+TGG 0.598164 3.2:-73958286 MS.gene066800:CDS
AATCGATCCCATAACCCTAT+CGG 0.598439 3.2:+73958327 None:intergenic
AGGACAGTGCAAGAGATGAT+GGG 0.601662 3.2:-73958294 MS.gene066800:CDS
TATTGTTTGATCTTGAAACA+TGG 0.608054 3.2:+73958397 None:intergenic
AGTAGTAGTAGCCTTAGGAA+TGG 0.626005 3.2:+73957906 None:intergenic
TTGCAATGAGTACAATTGAG+TGG 0.632366 3.2:-73958242 MS.gene066800:CDS
CTACCGATTGAAGGAGTAGG+TGG 0.639864 3.2:-73958207 MS.gene066800:CDS
AAGGAGGGGAAGAACACCAT+GGG 0.642482 3.2:-73958178 MS.gene066800:CDS
AATGGTCTAAGAGCTATGGT+AGG 0.662268 3.2:-73958011 MS.gene066800:CDS
CCTACTCCTTCAATCGGTAG+CGG 0.682306 3.2:+73958210 None:intergenic
TACCGATTGAAGGAGTAGGT+GGG 0.683545 3.2:-73958206 MS.gene066800:CDS
GAAGGAGGGGAAGAACACCA+TGG 0.687857 3.2:-73958179 MS.gene066800:CDS
GGGATCGATTTCCTACGACA+AGG 0.701576 3.2:-73958314 MS.gene066800:CDS
TATGCCTGGTATGAACAAAG+AGG 0.732695 3.2:-73958115 MS.gene066800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATTGTTTGATCTTGAAACA+TGG + chr3.2:73957998-73958017 None:intergenic 25.0%
AACATTAAGATCAAGGACTT+TGG + chr3.2:73958516-73958535 None:intergenic 30.0%
ACAAAGAGGATGTTAAAGTT+TGG - chr3.2:73958291-73958310 MS.gene066800:CDS 30.0%
AGAATGATGAAGATGAAGAA+TGG - chr3.2:73958363-73958382 MS.gene066800:CDS 30.0%
ATCTTCATCATTCTTCTTCT+TGG + chr3.2:73958357-73958376 None:intergenic 30.0%
CAAAGAGGATGTTAAAGTTT+GGG - chr3.2:73958292-73958311 MS.gene066800:CDS 30.0%
CACTCTTTATCCTTAAATCT+TGG - chr3.2:73957879-73957898 MS.gene066800:CDS 30.0%
TCAATTGTACTCATTGCAAA+CGG + chr3.2:73958149-73958168 None:intergenic 30.0%
TCTTTACATAACCATTCCTA+AGG - chr3.2:73958475-73958494 MS.gene066800:CDS 30.0%
TGTGCAGTTTGAGAATATTA+AGG - chr3.2:73958433-73958452 MS.gene066800:CDS 30.0%
AAAAACTCTCTTCTTGGGTT+CGG + chr3.2:73958036-73958055 None:intergenic 35.0%
AGTTTGTCTTGATGAACTCT+TGG + chr3.2:73957925-73957944 None:intergenic 35.0%
CAATTGTACTCATTGCAAAC+GGG + chr3.2:73958148-73958167 None:intergenic 35.0%
CAGGTTAAGACCAAGATTTA+AGG + chr3.2:73957892-73957911 None:intergenic 35.0%
GAATAAGTAGTAGTAGCCTT+AGG + chr3.2:73958494-73958513 None:intergenic 35.0%
GAATATTAAGGCTGAGGTTA+AGG - chr3.2:73958445-73958464 MS.gene066800:CDS 35.0%
GAGAAAGTGAATACAAGATG+AGG - chr3.2:73958249-73958268 MS.gene066800:CDS 35.0%
GTTTGAGAATATTAAGGCTG+AGG - chr3.2:73958439-73958458 MS.gene066800:CDS 35.0%
TGATGAACTCTTGGAAATCA+TGG + chr3.2:73957916-73957935 None:intergenic 35.0%
TTCTCAAACTGCACATTCTT+TGG + chr3.2:73958428-73958447 None:intergenic 35.0%
TTGCAATGAGTACAATTGAG+TGG - chr3.2:73958150-73958169 MS.gene066800:CDS 35.0%
AACTCTTGGAAATCATGGAC+AGG + chr3.2:73957911-73957930 None:intergenic 40.0%
AAGATGAGGTTTGATATGCC+TGG - chr3.2:73958263-73958282 MS.gene066800:CDS 40.0%
AATCGATCCCATAACCCTAT+CGG + chr3.2:73958068-73958087 None:intergenic 40.0%
AATGGTCTAAGAGCTATGGT+AGG - chr3.2:73958381-73958400 MS.gene066800:CDS 40.0%
ACACCATGGGAGATTAAAGA+AGG - chr3.2:73958227-73958246 MS.gene066800:CDS 40.0%
ACATCCTCTTTGTTCATACC+AGG + chr3.2:73958284-73958303 None:intergenic 40.0%
AGAGGATGTTAAAGTTTGGG+TGG - chr3.2:73958295-73958314 MS.gene066800:CDS 40.0%
AGCAGGAAAAACTCTCTTCT+TGG + chr3.2:73958042-73958061 None:intergenic 40.0%
ATTAAGGCTGAGGTTAAGGA+TGG - chr3.2:73958449-73958468 MS.gene066800:CDS 40.0%
GAAGAATGGTCTAAGAGCTA+TGG - chr3.2:73958377-73958396 MS.gene066800:CDS 40.0%
GCAGGAAAAACTCTCTTCTT+GGG + chr3.2:73958041-73958060 None:intergenic 40.0%
TATGCCTGGTATGAACAAAG+AGG - chr3.2:73958277-73958296 MS.gene066800:CDS 40.0%
TCTCCTTCTTTAATCTCCCA+TGG + chr3.2:73958233-73958252 None:intergenic 40.0%
TTAAGGCTGAGGTTAAGGAT+GGG - chr3.2:73958450-73958469 MS.gene066800:CDS 40.0%
! AGTAGTAGTAGCCTTAGGAA+TGG + chr3.2:73958489-73958508 None:intergenic 40.0%
AAGGACAGTGCAAGAGATGA+TGG - chr3.2:73958097-73958116 MS.gene066800:CDS 45.0%
AAGGAGTAGGTGGGTATAGA+AGG - chr3.2:73958195-73958214 MS.gene066800:CDS 45.0%
AGGACAGTGCAAGAGATGAT+GGG - chr3.2:73958098-73958117 MS.gene066800:CDS 45.0%
AGTAGGTGGGTATAGAAGGA+GGG - chr3.2:73958199-73958218 MS.gene066800:CDS 45.0%
TAAGGCTGAGGTTAAGGATG+GGG - chr3.2:73958451-73958470 MS.gene066800:CDS 45.0%
TACCCACCTACTCCTTCAAT+CGG + chr3.2:73958191-73958210 None:intergenic 45.0%
TTCTCTTGCTTCACCTGTCA+TGG + chr3.2:73957967-73957986 None:intergenic 45.0%
TTGGGTGGAAGAGAAGATGT+TGG - chr3.2:73958310-73958329 MS.gene066800:CDS 45.0%
! GGGAACAATGGAAAGCATGA+TGG - chr3.2:73958118-73958137 MS.gene066800:CDS 45.0%
! TACCGATTGAAGGAGTAGGT+GGG - chr3.2:73958186-73958205 MS.gene066800:CDS 45.0%
!! GCAAGAGATGATGGGAACAA+TGG - chr3.2:73958106-73958125 MS.gene066800:CDS 45.0%
!! TTTTTCCTGCTGCACCGATA+GGG - chr3.2:73958051-73958070 MS.gene066800:CDS 45.0%
AAGGAGGGGAAGAACACCAT+GGG - chr3.2:73958214-73958233 MS.gene066800:CDS 50.0%
CCTACTCCTTCAATCGGTAG+CGG + chr3.2:73958185-73958204 None:intergenic 50.0%
CTTCGAGTCAAAGCCATGAC+AGG - chr3.2:73957951-73957970 MS.gene066800:CDS 50.0%
GAGTAGGTGGGTATAGAAGG+AGG - chr3.2:73958198-73958217 MS.gene066800:CDS 50.0%
GTAGGTGGGTATAGAAGGAG+GGG - chr3.2:73958200-73958219 MS.gene066800:CDS 50.0%
TCTTGCACTGTCCTTGTCGT+AGG + chr3.2:73958092-73958111 None:intergenic 50.0%
! CTACCGATTGAAGGAGTAGG+TGG - chr3.2:73958185-73958204 MS.gene066800:CDS 50.0%
! GGGATCGATTTCCTACGACA+AGG - chr3.2:73958078-73958097 MS.gene066800:CDS 50.0%
! TGCTGCACCGATAGGGTTAT+GGG - chr3.2:73958058-73958077 MS.gene066800:CDS 50.0%
!! CTGTCATGGCTTTGACTCGA+AGG + chr3.2:73957953-73957972 None:intergenic 50.0%
!! GTTTTTCCTGCTGCACCGAT+AGG - chr3.2:73958050-73958069 MS.gene066800:CDS 50.0%
CATAACCCTATCGGTGCAGC+AGG + chr3.2:73958059-73958078 None:intergenic 55.0%
CTCTCTTCTTGGGTTCGGAC+TGG + chr3.2:73958031-73958050 None:intergenic 55.0%
GAAGGAGGGGAAGAACACCA+TGG - chr3.2:73958213-73958232 MS.gene066800:CDS 55.0%
! CCGCTACCGATTGAAGGAGT+AGG - chr3.2:73958182-73958201 MS.gene066800:CDS 55.0%
! CTGCTGCACCGATAGGGTTA+TGG - chr3.2:73958057-73958076 MS.gene066800:CDS 55.0%
! TCAATCGGTAGCGGCGATGA+AGG + chr3.2:73958176-73958195 None:intergenic 55.0%
! TCATCGCCGCTACCGATTGA+AGG - chr3.2:73958176-73958195 MS.gene066800:CDS 55.0%
! TCTTCTTGGGTTCGGACTGG+CGG + chr3.2:73958028-73958047 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 73957873 73958541 73957873 ID=MS.gene066800
chr3.2 mRNA 73957873 73958541 73957873 ID=MS.gene066800.t1;Parent=MS.gene066800
chr3.2 exon 73957873 73958541 73957873 ID=MS.gene066800.t1.exon1;Parent=MS.gene066800.t1
chr3.2 CDS 73957873 73958541 73957873 ID=cds.MS.gene066800.t1;Parent=MS.gene066800.t1
Gene Sequence

>MS.gene066800

ATGGCACACTCTTTATCCTTAAATCTTGGTCTTAACCTGTCCATGATTTCCAAGAGTTCATCAAGACAAACTCATTTCCTTCGAGTCAAAGCCATGACAGGTGAAGCAAGAGAAAAACTTGACCATGTTTCAAGATCAAACAATATTAAACACCGCCAGTCCGAACCCAAGAAGAGAGTTTTTCCTGCTGCACCGATAGGGTTATGGGATCGATTTCCTACGACAAGGACAGTGCAAGAGATGATGGGAACAATGGAAAGCATGATGGAAGACCCGTTTGCAATGAGTACAATTGAGTGGCCTTCATCGCCGCTACCGATTGAAGGAGTAGGTGGGTATAGAAGGAGGGGAAGAACACCATGGGAGATTAAAGAAGGAGAAAGTGAATACAAGATGAGGTTTGATATGCCTGGTATGAACAAAGAGGATGTTAAAGTTTGGGTGGAAGAGAAGATGTTGGTTGTGAAAGCTGAGAAAGCATCCAAGAAGAAGAATGATGAAGATGAAGAATGGTCTAAGAGCTATGGTAGGTATAGTAGCAGAATTGCTTTGCCAAAGAATGTGCAGTTTGAGAATATTAAGGCTGAGGTTAAGGATGGGGTTCTTTACATAACCATTCCTAAGGCTACTACTACTTATTCCAAAGTCCTTGATCTTAATGTTCAATGA

Protein sequence

>MS.gene066800.t1

MAHSLSLNLGLNLSMISKSSSRQTHFLRVKAMTGEAREKLDHVSRSNNIKHRQSEPKKRVFPAAPIGLWDRFPTTRTVQEMMGTMESMMEDPFAMSTIEWPSSPLPIEGVGGYRRRGRTPWEIKEGESEYKMRFDMPGMNKEDVKVWVEEKMLVVKAEKASKKKNDEDEEWSKSYGRYSSRIALPKNVQFENIKAEVKDGVLYITIPKATTTYSKVLDLNVQ