AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072766


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072766.t1 MTR_5g010010 96.380 221 8 0 13 233 13 233 2.48e-155 430
MS.gene072766.t1 MTR_7g053480 70.297 101 30 0 1 101 1 101 7.54e-41 136
MS.gene072766.t1 MTR_3g110020 70.588 51 15 0 1 51 1 51 1.35e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072766.t1 AT1G63970 83.237 173 29 0 61 233 59 231 1.10e-105 305
MS.gene072766.t1 AT1G63970 81.818 99 18 0 61 159 59 157 3.43e-56 176

Find 61 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGGTTATCCTTTAATTAT+TGG 0.154047 5.3:+3738389 MS.gene072766:CDS
TTAATTCCACCAATAATTAA+AGG 0.221109 5.3:-3738398 None:intergenic
TCACTGTTTGTTTCAGCTTC+AGG 0.284381 5.3:+3738254 MS.gene072766:CDS
CGCTTCTGTGGTAAATATAA+AGG 0.303097 5.3:+3744917 MS.gene072766:CDS
AAAGAATAAGAGAATGGAAT+TGG 0.329681 5.3:-3738191 None:intergenic
CATGAGGCAGGTTATGATAT+TGG 0.350566 5.3:+3744801 MS.gene072766:CDS
GCTTTAGGTCTTCCTGATAT+AGG 0.382227 5.3:+3739423 MS.gene072766:CDS
TTGAAGGAGTAGCGGAGATT+GGG 0.383157 5.3:-3738316 None:intergenic
TGGAATTGGGTGAGAAGATT+TGG 0.392377 5.3:-3738177 None:intergenic
TCCTTGTGTGGGCTTAGTTT+CGG 0.392429 5.3:-3744855 None:intergenic
TTTGAAGGAGTAGCGGAGAT+TGG 0.395299 5.3:-3738317 None:intergenic
AAGAATAAGAGAATGGAATT+GGG 0.439995 5.3:-3738190 None:intergenic
ATCGATTTCAACTGAGGCTG+TGG 0.440478 5.3:-3738288 None:intergenic
GTGGAAGGGTTGTGATTCTT+CGG 0.447373 5.3:+3739473 MS.gene072766:CDS
GGTTATCCTTTAATTATTGG+TGG 0.460157 5.3:+3738392 MS.gene072766:CDS
AGAATATACTCACAGATTCA+TGG 0.463953 5.3:-3739503 None:intergenic
CATGAAAAGGTTGATAGCCT+TGG 0.468863 5.3:+3744948 MS.gene072766:CDS
CCCACACAAGGATGCTATCA+AGG 0.475088 5.3:+3744866 MS.gene072766:CDS
AGAGGAAAAGAATAAGAGAA+TGG 0.478928 5.3:-3738197 None:intergenic
AAACCATGGCCAACCCGAAA+AGG 0.483370 5.3:-3738347 None:intergenic
GATTTGGTGGGTGTTGTGAA+TGG 0.484659 5.3:-3738161 None:intergenic
GCCTTGATAGCATCCTTGTG+TGG 0.485497 5.3:-3744867 None:intergenic
TCCAAGCAGTGCAGATAAGT+TGG 0.486196 5.3:-3744889 None:intergenic
TTCCACTTAGGATCAGAATC+AGG 0.496430 5.3:-3739456 None:intergenic
ATTTGGTGGGTGTTGTGAAT+GGG 0.497158 5.3:-3738160 None:intergenic
GACCTTCATCGATTGGAACC+TGG 0.499572 5.3:+3738371 MS.gene072766:CDS
CCTTGATAGCATCCTTGTGT+GGG 0.507271 5.3:-3744866 None:intergenic
CGAAAAGGAAGAACCTTTGA+AGG 0.510185 5.3:-3738332 None:intergenic
ATTAATATACCTCATGATAG+AGG 0.512773 5.3:+3738416 MS.gene072766:CDS
GCCAACTTATCTGCACTGCT+TGG 0.513880 5.3:+3744888 MS.gene072766:CDS
TTCCTGATTCTGATCCTAAG+TGG 0.534340 5.3:+3739454 MS.gene072766:CDS
ATGATTTGAGATGGAGGGTA+TGG 0.536370 5.3:-3738226 None:intergenic
GTCAGACTTATGCATGAGGC+AGG 0.542152 5.3:+3744789 MS.gene072766:CDS
TCGATTTCAACTGAGGCTGT+GGG 0.542972 5.3:-3738287 None:intergenic
CCAATAATTAAAGGATAACC+AGG 0.547141 5.3:-3738389 None:intergenic
GAATCACAACCCTTCCACTT+AGG 0.553850 5.3:-3739468 None:intergenic
TGATTCTGATCCTAAGTGGA+AGG 0.553947 5.3:+3739458 MS.gene072766:CDS
AATCTCCGCTACTCCTTCAA+AGG 0.556988 5.3:+3738319 MS.gene072766:CDS
AACCAGGTTCCAATCGATGA+AGG 0.565616 5.3:-3738373 None:intergenic
GGATTTATCGATTTCAACTG+AGG 0.577148 5.3:-3738294 None:intergenic
TGGAGGGTATGGTGTTTGAG+AGG 0.579851 5.3:-3738215 None:intergenic
TGAAGGAGTAGCGGAGATTG+GGG 0.589337 5.3:-3738315 None:intergenic
GGTTGCGAGGCTCATTCTGA+TGG 0.591970 5.3:+3738437 MS.gene072766:CDS
GGTGGGTGTTGTGAATGGGA+AGG 0.594415 5.3:-3738156 None:intergenic
AATTGGGTGAGAAGATTTGG+TGG 0.594739 5.3:-3738174 None:intergenic
ATTGGGTGAGAAGATTTGGT+GGG 0.596035 5.3:-3738173 None:intergenic
GCTTGGAGTTGACGCTTCTG+TGG 0.600936 5.3:+3744905 MS.gene072766:CDS
GGATGTTCTGCTTCACTGCG+TGG 0.611052 5.3:+3739383 MS.gene072766:CDS
AAGAACCTTTGAAGGAGTAG+CGG 0.614741 5.3:-3738324 None:intergenic
TGGTGATGATTTGAGATGGA+GGG 0.617114 5.3:-3738231 None:intergenic
ACCTCATGATAGAGGTTGCG+AGG 0.621758 5.3:+3738424 MS.gene072766:CDS
GCCGAAACTAAGCCCACACA+AGG 0.626457 5.3:+3744854 MS.gene072766:CDS
ATGCAACATTGATACTTCAG+AGG 0.627600 5.3:+3744832 MS.gene072766:CDS
GATTCTGATCCTAAGTGGAA+GGG 0.630398 5.3:+3739459 MS.gene072766:CDS
ATCGATGAAGGTCAAAACCA+TGG 0.631860 5.3:-3738361 None:intergenic
GTGATGGTGATGATTTGAGA+TGG 0.635547 5.3:-3738235 None:intergenic
ATGGTGATGATTTGAGATGG+AGG 0.646685 5.3:-3738232 None:intergenic
GCCTCGCAACCTCTATCATG+AGG 0.647110 5.3:-3738425 None:intergenic
TGTTAGAGAAGATGAAGACA+TGG 0.663229 5.3:-3738120 None:intergenic
GAAGCTGAAACAAACAGTGA+TGG 0.680351 5.3:-3738251 None:intergenic
ACAGGTCAGACTTATGCATG+AGG 0.696663 5.3:+3744785 MS.gene072766:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.3 gene 3738122 3745022 3738122 ID=MS.gene072766
chr5.3 mRNA 3738122 3745022 3738122 ID=MS.gene072766.t1;Parent=MS.gene072766
chr5.3 exon 3738122 3738458 3738122 ID=MS.gene072766.t1.exon1;Parent=MS.gene072766.t1
chr5.3 CDS 3738122 3738458 3738122 ID=cds.MS.gene072766.t1;Parent=MS.gene072766.t1
chr5.3 exon 3739382 3739512 3739382 ID=MS.gene072766.t1.exon2;Parent=MS.gene072766.t1
chr5.3 CDS 3739382 3739512 3739382 ID=cds.MS.gene072766.t1;Parent=MS.gene072766.t1
chr5.3 exon 3744789 3745022 3744789 ID=MS.gene072766.t1.exon3;Parent=MS.gene072766.t1
chr5.3 CDS 3744789 3745022 3744789 ID=cds.MS.gene072766.t1;Parent=MS.gene072766.t1
Gene Sequence

>MS.gene072766

ATGTCTTCATCTTCTCTAACAGCTTCTTCTTACTCCTTCCCATTCACAACACCCACCAAATCTTCTCACCCAATTCCATTCTCTTATTCTTTTCCTCTCAAACACCATACCCTCCATCTCAAATCATCACCATCACTGTTTGTTTCAGCTTCAGGAGCTGCAACACCCACAGCCTCAGTTGAAATCGATAAATCCCCAATCTCCGCTACTCCTTCAAAGGTTCTTCCTTTTCGGGTTGGCCATGGTTTTGACCTTCATCGATTGGAACCTGGTTATCCTTTAATTATTGGTGGAATTAATATACCTCATGATAGAGGTTGCGAGGCTCATTCTGATGGTATTCATTCTATCTAACCCTTATAGCATTTTCATTTTTATAATCTTTTTTACATTTCTCCGAGTTAATAGTCATTTTGATTCTTAAATGTGTGAGACACTGTCACAATTGTTCTTCAATGTATAAAGATTTCAACAACATACATAAATTTGTCTTTTTTTGTTGGTTGGTTAGTTAGTGAATATTTACTGTTTGTCAATTTAGTCCATAAAATTTATTAACGAAAGATAAACTCCTATGCTGTTTTTGTAATTTCGAAATATTTAGGGACTATGACTCTGTTTGGTAGAAAGTAGTGGATAGTGGATAAGTTAGTTGATAGTGGATGGACTTATAGCAGATAAGCTAGCTGATAGTGGATAAACTAGTGAATTAAATTTGTAGTGTTTTGAAGATTATACGGTATAAGCTATCCATTGTAAGTTTGTGATTAGGGGCCGTTTGTTTGGATTGGCTTATTTACTATGAAACACGGACACTCCACAAATTAGGTGTGTTCCGGTGTCGGACATGCTTCGCTTCCTACACTGGCACATATGATTACACTAAATTATGTCATTTTCTCAAAATTATTATCGACATCGGTGTCATGTCCCGTGTCCGTGTCTATGTTTGTGCTTCATAGCTTATTTTTTAGCTTATGAAAAATAGTTTATGCAAATAAATAAGTTGTTATGTTAATTCATAAGTTCTCACTAACGAAAATTGTATTTTCATAAGCCGTTTTCTCATAAACTATCTTGACGAGCTTATGAATAATACATAAAAGCTTATTTATTTTCATAAGCTGTTCTGGATAAGCTAAAAAATAAGCCAATCCAAATGGGCCCAAGATCTTGCCAACAACTCTATACCAAATTGAGTATTTACTCAATTTTTTCATTTTTTTTCTGAGTTTGAACATGATGATGAAATATGAACAGGGGATGTTCTGCTTCACTGCGTGGTTGATGCAATTTTAGGGGCTTTAGGTCTTCCTGATATAGGACAGATTTTTCCTGATTCTGATCCTAAGTGGAAGGGTTGTGATTCTTCGGTGTTTGTCCATGAATCTGTGAGTATATTCTACATAAATATCTTCCTTCTACTTTATTTTCTCAATTGAAATACAGTATGATTTAGGCCTTAGTGTAATTGTGTTTTCAGTTTTTCTAATGTTGTTTGAATTTAGGGAATATATTAATAGGTTACCAAATGTTCACCGTAATGATTTGTTTGTTCTGTCACAAATTGTAATTCCATAATTATTCATGGACACATTGCATATTGCTTTCAAGTTATTATTATGCAGTGTTAGAACTTAAATGGTTGCTAAAGTATAAGCATGATAGGAAGGTATGCCTTGCAATCAGGGACAGTGGACAGTGGATTGTTCGGATTGTTAGAATAAGATGGAATGAAATGAGATATCAAATGCATTCCATCTGCTACATCTATTTTTCATTCTTCTAATTTAGGGTGCATGGATGAAATGACATGACTTTATTATTGAACGAGTTTGGTACGTACGAGTTTAAGAAATCATGAAATTATTACTGAGTTAAAAGGGTAAGGACTTAGAAGAGATTAGCGCTTTGGTTAACAGTGGAAATAATGACATGAGTTAACAGGCTACTGATTAGCTTGATGAGCAGAAAAGCAGACTGTTCATGTACCAATCATATACCTTGTATGTATATAAGGGAATCAGCTGTGTTCAGTTTTTTTAAAGGGGACGGGAGAAGAGAGGAAGGAGAGCAAAATCTCGCATAATACTATAACATTATAAATAGACTAAATTAACCTTAAATACCACACGAAATGTTAAAATGGTGTAAAATCTAAATTTGTATGTTCTTTTCATTAAAATTACTCATATCTCCGTATTATACCAAACAAAAAAGAGAGATGTTTTTATTCCCCTCTGTTAGAACCGAATAGACCTGTAGAGGCGGGTATGCATACCAGCTCGCACCAGTCAGCATAGGCCCATATTTAAACTGATTGGTTTGCATAATCCCTTATTTAAACAGGCATATATTTTGTTGGATCTTGTGTTGAGGGTCTGCTGCAATAACTTGACCGAGAAGATGATGAAGATGAATACGATGAAGTGTGCACCTAAATTCCAAAGATTGTTGTATATCTTGATTGTTGGGAAGAGGCTGCTGCACTTGAAACCTGAAAAGTTTAATAGTTATGCATGTAAACTCAAAGCGTATGGGGTTGCCATACTAACCTTTAGGTTCGATTCGCCTCCACCAATAACTGTATATTGGATGCAATAAGGAATGACCGGCGAATCCCCTTCAGGATACTATGAGTTCCTTGACGTGTATTGCACAACCACACCAAGCGTATCTCCTAACAGTATTTTACAGATTTATGGTTCCAGCAATTCACTCATGCATTAGAGAATTGATGGATGATTTAGAGTAAGAGAATGATGGAGAATTATGTTGTTGTTCTTTTTTTTTGTGTGTGTCCAACTAATGCCCAAATGCCAAACATTTATAGTGCATTATATGACTCTTCACATGAGAGAAAACACCCCTTTGACTTGAATCAATGCTATGATTCATTAAGCACCCATTTGCCAAAGATTATGATCATATACACTTGAATCAATGTTATGGTTCATTAAGCACCCTTTTACCAAAGGTTATAACCATATACACTTGAATCAATGTTATGGTTCATTAAGCACCCTTTTGCCAAGTGTTATAACCATATACATTTGAATCAATGCAAATGTTCATTAAGCATCTCTTTGCCAAAGGTTCCAATGTATATGAATGATTCCCATAAAGAGTTACAACTTTTGGTTTTCTAAAGTGATTCAATTATTTTGGAAATCACCAACAATCCCCCACCATTTTCAAAATACTAAATCACATAAATCCGGAAATTCCATGCCTTCAGATAATGGTATCTCGCGATTTGAACCATTACTTAGTAAGTTAAGTTTTCGCTCATTCCCAAATAGTATGGTAGGCAAGCTTTGAACCAACTATTCATTAGAACAAGTGTGCATAACTTACACATAGAATCTCGGTACCATTGATAAGGAGACTTGTAAGATGACACATTAAGAAGGCCTTGTGTCATATATCCCTTTTCATGAGAATTTAAGAGTCAAACCCTTGAACTCCGTGAAGCGGCCAAACTTCATTCTCACATAGGTAGACTATATCGGAAATGCCCCATAAGTATGCATTCCAGCAAATATATGCTATATGTCCATTAAGAGGAAAACCTCATCCTACCCTTTTTCTTCTTATGGTCCAAGTACTTTTTCCCCTTGGGATGTTATCTATTGTCAAGTACTTCAATTACTCTAATAGTGTACTTTCACCATTGAACCCAAGACTTGATGCTACTCAAGTGTGGGTTGAGTTTCCACCATTGGTAATCAATTAGATTTGGGTTTGTACCACATCCCTAATAATGTCTTCAATGTATTCAACATCATTTCCAAAGACATCATTCCTTGTCAATCATTTCGTCAAATAATTGCAAGATTTTCTTTTAACTTTTATAAACTTTGAATATTCAATTCTTCACATAGATTTTGTATTCTCATAGCTATGTCTATGGATCAACTTATATACTCTTGATAGTATTCACCAACTATCATCAAACCATGACACACTTTTGTCAATTGAGTATATTGTAATAATGCACAAACTTTTATGTCATTACACCTTTGTTATAACAATATTTAACATGCATTTCAATTTGAACTTCAACTTTTCTTTTATATCTAGCCAAGATATATCATACATTGAATGCATTAAACATACATAGCAAAATAAAAATTTATCATAAAAGAATGTGATAACCAAACTTCATAAGATGAGATTAAACATGTAAGTCATATACTCCACATAAAATTAAATATAACACAATCATTTTGATTTTAGTTATCATATAACTAATACATATATCATAAATTCATTGTGTTAAATTCAAGTTTATGTTTAAATATGTTCAATACTAAATTTCAATAAACAATGACATAGAAGAATAAACAATGAACCAAAATCATTAATATTTTCTATGACTTTCTTCCATGAACATATATCAACTTACAATAATTCAAAATATACCCAAATTATTTTAATGTCATGATAAACAAGAAATTAACAATTACTTTTCATATTATCATGTACATATAATATCAACACAATATTAATGATAATAATTGCTTACACAAAGTAAATAATATCAATAAACCAACCATTTGATATTTTGACAATTTATCTACACTACTTTCATAAATAAAATAGTGTTTAAACATTCTTAACATATTACTATACTCATCAAAATTCATATACTTCAACAATTTCTAAATCATGTATATATATGTCACCACATGTTAAATCTAACATATTCATATTTACTTAGATACTACACACATGCATTTAGAACACCAAATATTTCATAATAGGTAATCACTTATATGCACATTATCTCATGTTCAAATTAACATCAAACAGATTTCAAACGATAAATTTCATACATATCAAAATTTATCATGCATATATTGTCTACAACCACATGTAAATTTAACATAAACACATCACATGTGAAATATTTATCCATAAATTTCCACAACATATGTTCATAAAAGGAATTTGTTTACCGTTCACCGTAGTCATGGATGAAAATGTAAACTTTCATCTCTTCCAAAATTGCAGCCATTAGTCACAAGTAAATCATAAATCGAACCTAAAGATTGTTATATATCTTGTTTGTTGGGAAGAAGCTGCTACACTTGATACCTGAAATGTTTAATGATTATGCATGTAAAACTCAAAGCGTATGGGGTTGCCACACTAACCTTTAGGTTCGATTCGCCCCCACCAATAACTGTATATTGGATGCAATAAGGAATGACCGGCGAATCCCCTTCAGGATACTATGAGTTCCTTGACGTGTATTGCACAACCACACCAAGCGTATCTCCTAACAGTATTTTACAGATTTATGGTTCCAGCAATTCACTCATGCATTAGAGAATTGATGGATGATTTAGAGTAAGAGAATGATGGAGAATTATGTTGTTGTTCTTTTTTTTTGTGTGTGTCCAACTAATGCCCAAATGCCAAACATTTATAGTGCATTATATGACTCTTCACATGAGAGAAAACACCCCTTTGACTTGAATCAATGCTATGATTCATTAAGCACCCATTTGCCAAAGATTATGATCATATACACTTGAATCAATGTTATGGTTCATTAAGCACCCTTTTACCAAAGGTTATAACCATATACACTTGAATCAATGTTATGGTTCATTAAGCACCCTTTTGCCAAGTGTTATAACCATATACATTTGAATCAATGCAAATGTTCATTAAGCATCTCTTTGCCAAAGGTTCCAATGTATATGAATGATTCCCATAAAGAGTTACAACTTTTGGTTTTCTAAAGTGATTCAATTATTTTGGAAATCACCAACATATTTAAGAAGTTCGCATACATATGTGAGTTCAAAAGGCCGGTCTGCCTACTAATGTGCATGTTTTACTTTTCCTTGTAAAAAAATTTTTGGGTAATTTTCACTTGAAAAGTAACTTAAAAATTGTTTTTCTTTTTTATGTAGATATATGGGTTGATTTGCAAAGTTGTTCATCAAACCCATTTAATCCGGTGTCACAAACTAACATACATAGGATCACAAGGTCAGCTATTTAACTGGGCACAAATTTACATCTCCCAAACAACCGTTAGCCCTTGTACTCACCTCAACATACACACATGATACACCTCAAATATGTCATTTCCGAAGAAAAAGCTTTTATTCACTCTACAGACAGTTAGCATGTGAAGTGTGACTTCCTATTTTAAACAATTCAGATACAATTCACATCTGAAAAATGAGTAATTATTCTAACAAGTTCTATCAATTTAAGCTGATACAGACCAAAAAGTAGAGAATGAGTTCAAGGATATTACAGTAAGAAAAAATTACCTAACAGTATTCAGAAGTGAGATTGTGCTCTATCAGTCAAGTGTGTAACAGGGTAATTGATAATGTCTCTTAACCAAACAAAACTTTGCTATTTTTTTTTTTTTTAGTTAAATTTTTTGCTAGTTATTGAATAAGTAAACTGTGACCTAACAGTGACTCCTCTTTTATTGTATTAATTTTGGACCCTGCATTTACCATGTGTTTAGCAGTTATTAAACAACATTTTCTGTGATTTTCTTTTGACGTATGATGGTTTTACAAATTATATTTGGTTACTGAAAGTGTTTTCTTAGGATTATGATTAAAGATAACGCTGTATCTATACTTGATTTTTTTAGGGTATTTTATAATATGTTATGATTTTATTACAGGTCAGACTTATGCATGAGGCAGGTTATGATATTGGAAATTTAGATGCAACATTGATACTTCAGAGGCCGAAACTAAGCCCACACAAGGATGCTATCAAGGCCAACTTATCTGCACTGCTTGGAGTTGACGCTTCTGTGGTAAATATAAAGGCAAAAACTCATGAAAAGGTTGATAGCCTTGGAGAAAATAGAAGTATAGCAGCTCACACAGTTGTTCTTCTAATGAAGAAATGA

Protein sequence

>MS.gene072766.t1

MSSSSLTASSYSFPFTTPTKSSHPIPFSYSFPLKHHTLHLKSSPSLFVSASGAATPTASVEIDKSPISATPSKVLPFRVGHGFDLHRLEPGYPLIIGGINIPHDRGCEAHSDGDVLLHCVVDAILGALGLPDIGQIFPDSDPKWKGCDSSVFVHESVRLMHEAGYDIGNLDATLILQRPKLSPHKDAIKANLSALLGVDASVVNIKAKTHEKVDSLGENRSIAAHTVVLLMKK