Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79304.t1 | XP_013452555.1 | 95.5 | 242 | 11 | 0 | 1 | 242 | 1 | 242 | 3.40E-123 | 451.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79304.t1 | G7ZX37 | 95.5 | 242 | 11 | 0 | 1 | 242 | 1 | 242 | 2.4e-123 | 451.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene79304 | 0.837207 | 5.96E-57 | -1.69E-46 |
| MS.gene049171 | MS.gene79304 | 0.824498 | 7.81E-54 | -1.69E-46 |
| MS.gene049217 | MS.gene79304 | 0.823152 | 1.62E-53 | -1.69E-46 |
| MS.gene049222 | MS.gene79304 | 0.863152 | 3.05E-64 | -1.69E-46 |
| MS.gene049285 | MS.gene79304 | 0.821999 | 2.99E-53 | -1.69E-46 |
| MS.gene049320 | MS.gene79304 | 0.827546 | 1.47E-54 | -1.69E-46 |
| MS.gene049356 | MS.gene79304 | 0.819769 | 9.76E-53 | -1.69E-46 |
| MS.gene049369 | MS.gene79304 | 0.820662 | 6.09E-53 | -1.69E-46 |
| MS.gene049396 | MS.gene79304 | 0.822838 | 1.91E-53 | -1.69E-46 |
| MS.gene049427 | MS.gene79304 | 0.820535 | 6.52E-53 | -1.69E-46 |
| MS.gene049609 | MS.gene79304 | 0.880279 | 6.25E-70 | -1.69E-46 |
| MS.gene049805 | MS.gene79304 | 0.85513 | 7.75E-62 | -1.69E-46 |
| MS.gene050083 | MS.gene79304 | -0.800303 | 1.54E-48 | -1.69E-46 |
| MS.gene050397 | MS.gene79304 | 0.800921 | 1.15E-48 | -1.69E-46 |
| MS.gene050432 | MS.gene79304 | 0.831621 | 1.50E-55 | -1.69E-46 |
| MS.gene050529 | MS.gene79304 | 0.818952 | 1.50E-52 | -1.69E-46 |
| MS.gene050538 | MS.gene79304 | 0.873815 | 1.10E-67 | -1.69E-46 |
| MS.gene050593 | MS.gene79304 | 0.86362 | 2.19E-64 | -1.69E-46 |
| MS.gene050670 | MS.gene79304 | 0.876254 | 1.62E-68 | -1.69E-46 |
| MS.gene050686 | MS.gene79304 | 0.802077 | 6.66E-49 | -1.69E-46 |
| MS.gene050747 | MS.gene79304 | 0.870957 | 9.85E-67 | -1.69E-46 |
| MS.gene050772 | MS.gene79304 | 0.881247 | 2.81E-70 | -1.69E-46 |
| MS.gene050809 | MS.gene79304 | -0.821528 | 3.85E-53 | -1.69E-46 |
| MS.gene05080 | MS.gene79304 | 0.851623 | 7.86E-61 | -1.69E-46 |
| MS.gene050812 | MS.gene79304 | 0.80897 | 2.38E-50 | -1.69E-46 |
| MS.gene05081 | MS.gene79304 | 0.839971 | 1.15E-57 | -1.69E-46 |
| MS.gene050868 | MS.gene79304 | 0.826019 | 3.41E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene79304 | 0.824005 | 1.02E-53 | -1.69E-46 |
| MS.gene050950 | MS.gene79304 | 0.858389 | 8.52E-63 | -1.69E-46 |
| MS.gene05105 | MS.gene79304 | 0.891439 | 3.97E-74 | -1.69E-46 |
| MS.gene051228 | MS.gene79304 | 0.805248 | 1.46E-49 | -1.69E-46 |
| MS.gene051387 | MS.gene79304 | 0.838778 | 2.35E-57 | -1.69E-46 |
| MS.gene051617 | MS.gene79304 | 0.817463 | 3.25E-52 | -1.69E-46 |
| MS.gene051666 | MS.gene79304 | 0.901436 | 2.68E-78 | -1.69E-46 |
| MS.gene051694 | MS.gene79304 | 0.813308 | 2.73E-51 | -1.69E-46 |
| MS.gene051734 | MS.gene79304 | 0.827159 | 1.83E-54 | -1.69E-46 |
| MS.gene051838 | MS.gene79304 | 0.852351 | 4.89E-61 | -1.69E-46 |
| MS.gene051858 | MS.gene79304 | 0.856375 | 3.36E-62 | -1.69E-46 |
| MS.gene051874 | MS.gene79304 | 0.822867 | 1.88E-53 | -1.69E-46 |
| MS.gene052134 | MS.gene79304 | 0.809598 | 1.75E-50 | -1.69E-46 |
| MS.gene052135 | MS.gene79304 | 0.801929 | 7.14E-49 | -1.69E-46 |
| MS.gene052136 | MS.gene79304 | 0.815541 | 8.77E-52 | -1.69E-46 |
| MS.gene052164 | MS.gene79304 | 0.814666 | 1.37E-51 | -1.69E-46 |
| MS.gene052188 | MS.gene79304 | 0.811108 | 8.26E-51 | -1.69E-46 |
| MS.gene052310 | MS.gene79304 | 0.80958 | 1.76E-50 | -1.69E-46 |
| MS.gene052346 | MS.gene79304 | 0.82658 | 2.51E-54 | -1.69E-46 |
| MS.gene052379 | MS.gene79304 | 0.824298 | 8.71E-54 | -1.69E-46 |
| MS.gene05240 | MS.gene79304 | -0.802571 | 5.27E-49 | -1.69E-46 |
| MS.gene052414 | MS.gene79304 | 0.834336 | 3.18E-56 | -1.69E-46 |
| MS.gene052421 | MS.gene79304 | 0.84374 | 1.16E-58 | -1.69E-46 |
| MS.gene052481 | MS.gene79304 | 0.850053 | 2.18E-60 | -1.69E-46 |
| MS.gene052484 | MS.gene79304 | 0.85635 | 3.41E-62 | -1.69E-46 |
| MS.gene052485 | MS.gene79304 | 0.842075 | 3.23E-58 | -1.69E-46 |
| MS.gene052501 | MS.gene79304 | 0.837857 | 4.06E-57 | -1.69E-46 |
| MS.gene052503 | MS.gene79304 | 0.814825 | 1.26E-51 | -1.69E-46 |
| MS.gene052505 | MS.gene79304 | 0.833144 | 6.31E-56 | -1.69E-46 |
| MS.gene052506 | MS.gene79304 | 0.816087 | 6.63E-52 | -1.69E-46 |
| MS.gene052615 | MS.gene79304 | 0.834416 | 3.03E-56 | -1.69E-46 |
| MS.gene052822 | MS.gene79304 | 0.816704 | 4.82E-52 | -1.69E-46 |
| MS.gene052823 | MS.gene79304 | 0.806709 | 7.21E-50 | -1.69E-46 |
| MS.gene052825 | MS.gene79304 | 0.807311 | 5.38E-50 | -1.69E-46 |
| MS.gene052870 | MS.gene79304 | 0.83838 | 2.98E-57 | -1.69E-46 |
| MS.gene052872 | MS.gene79304 | 0.837031 | 6.61E-57 | -1.69E-46 |
| MS.gene052982 | MS.gene79304 | 0.840529 | 8.23E-58 | -1.69E-46 |
| MS.gene053103 | MS.gene79304 | 0.853244 | 2.72E-61 | -1.69E-46 |
| MS.gene053104 | MS.gene79304 | 0.889226 | 2.93E-73 | -1.69E-46 |
| MS.gene053107 | MS.gene79304 | 0.85319 | 2.82E-61 | -1.69E-46 |
| MS.gene053207 | MS.gene79304 | 0.83629 | 1.02E-56 | -1.69E-46 |
| MS.gene053209 | MS.gene79304 | 0.820101 | 8.19E-53 | -1.69E-46 |
| MS.gene053213 | MS.gene79304 | 0.848194 | 7.16E-60 | -1.69E-46 |
| MS.gene053286 | MS.gene79304 | 0.837948 | 3.85E-57 | -1.69E-46 |
| MS.gene053322 | MS.gene79304 | 0.834352 | 3.15E-56 | -1.69E-46 |
| MS.gene053436 | MS.gene79304 | 0.817691 | 2.89E-52 | -1.69E-46 |
| MS.gene053449 | MS.gene79304 | 0.841014 | 6.14E-58 | -1.69E-46 |
| MS.gene053479 | MS.gene79304 | 0.820291 | 7.41E-53 | -1.69E-46 |
| MS.gene053574 | MS.gene79304 | 0.874129 | 8.59E-68 | -1.69E-46 |
| MS.gene053579 | MS.gene79304 | 0.82632 | 2.89E-54 | -1.69E-46 |
| MS.gene053626 | MS.gene79304 | 0.808316 | 3.29E-50 | -1.69E-46 |
| MS.gene053632 | MS.gene79304 | 0.840418 | 8.80E-58 | -1.69E-46 |
| MS.gene053824 | MS.gene79304 | 0.862401 | 5.20E-64 | -1.69E-46 |
| MS.gene053916 | MS.gene79304 | 0.881509 | 2.26E-70 | -1.69E-46 |
| MS.gene054003 | MS.gene79304 | 0.807252 | 5.54E-50 | -1.69E-46 |
| MS.gene054408 | MS.gene79304 | 0.846625 | 1.93E-59 | -1.69E-46 |
| MS.gene054600 | MS.gene79304 | 0.838694 | 2.47E-57 | -1.69E-46 |
| MS.gene054642 | MS.gene79304 | 0.851511 | 8.46E-61 | -1.69E-46 |
| MS.gene054927 | MS.gene79304 | 0.828609 | 8.17E-55 | -1.69E-46 |
| MS.gene05494 | MS.gene79304 | 0.85245 | 4.58E-61 | -1.69E-46 |
| MS.gene054955 | MS.gene79304 | 0.817648 | 2.96E-52 | -1.69E-46 |
| MS.gene05505 | MS.gene79304 | 0.885063 | 1.12E-71 | -1.69E-46 |
| MS.gene055116 | MS.gene79304 | 0.844189 | 8.82E-59 | -1.69E-46 |
| MS.gene055206 | MS.gene79304 | 0.863387 | 2.58E-64 | -1.69E-46 |
| MS.gene055212 | MS.gene79304 | 0.829636 | 4.61E-55 | -1.69E-46 |
| MS.gene055224 | MS.gene79304 | 0.862459 | 4.99E-64 | -1.69E-46 |
| MS.gene055289 | MS.gene79304 | 0.890574 | 8.71E-74 | -1.69E-46 |
| MS.gene055362 | MS.gene79304 | 0.818626 | 1.78E-52 | -1.69E-46 |
| MS.gene055502 | MS.gene79304 | 0.830185 | 3.39E-55 | -1.69E-46 |
| MS.gene055577 | MS.gene79304 | 0.865005 | 8.08E-65 | -1.69E-46 |
| MS.gene055722 | MS.gene79304 | 0.85523 | 7.25E-62 | -1.69E-46 |
| MS.gene055741 | MS.gene79304 | 0.81228 | 4.59E-51 | -1.69E-46 |
| MS.gene055744 | MS.gene79304 | 0.812222 | 4.72E-51 | -1.69E-46 |
| MS.gene055748 | MS.gene79304 | 0.810868 | 9.31E-51 | -1.69E-46 |
| MS.gene055750 | MS.gene79304 | 0.817076 | 3.98E-52 | -1.69E-46 |
| MS.gene055909 | MS.gene79304 | 0.80368 | 3.10E-49 | -1.69E-46 |
| MS.gene055911 | MS.gene79304 | 0.804585 | 2.01E-49 | -1.69E-46 |
| MS.gene05592 | MS.gene79304 | 0.878945 | 1.86E-69 | -1.69E-46 |
| MS.gene055941 | MS.gene79304 | 0.853124 | 2.94E-61 | -1.69E-46 |
| MS.gene05602 | MS.gene79304 | 0.803863 | 2.85E-49 | -1.69E-46 |
| MS.gene056166 | MS.gene79304 | 0.804476 | 2.12E-49 | -1.69E-46 |
| MS.gene056374 | MS.gene79304 | 0.813984 | 1.94E-51 | -1.69E-46 |
| MS.gene056407 | MS.gene79304 | 0.819518 | 1.11E-52 | -1.69E-46 |
| MS.gene056422 | MS.gene79304 | 0.843877 | 1.07E-58 | -1.69E-46 |
| MS.gene056446 | MS.gene79304 | 0.81513 | 1.08E-51 | -1.69E-46 |
| MS.gene056448 | MS.gene79304 | 0.829721 | 4.39E-55 | -1.69E-46 |
| MS.gene056487 | MS.gene79304 | 0.81466 | 1.38E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene79304 | 0.857252 | 1.85E-62 | -1.69E-46 |
| MS.gene05664 | MS.gene79304 | 0.806347 | 8.60E-50 | -1.69E-46 |
| MS.gene056657 | MS.gene79304 | 0.827955 | 1.18E-54 | -1.69E-46 |
| MS.gene05666 | MS.gene79304 | 0.810594 | 1.07E-50 | -1.69E-46 |
| MS.gene056796 | MS.gene79304 | 0.807695 | 4.46E-50 | -1.69E-46 |
| MS.gene056897 | MS.gene79304 | 0.816658 | 4.94E-52 | -1.69E-46 |
| MS.gene056947 | MS.gene79304 | 0.843292 | 1.53E-58 | -1.69E-46 |
| MS.gene057011 | MS.gene79304 | 0.821699 | 3.52E-53 | -1.69E-46 |
| MS.gene057048 | MS.gene79304 | 0.80826 | 3.38E-50 | -1.69E-46 |
| MS.gene057076 | MS.gene79304 | 0.860007 | 2.79E-63 | -1.69E-46 |
| MS.gene057086 | MS.gene79304 | 0.805761 | 1.14E-49 | -1.69E-46 |
| MS.gene057124 | MS.gene79304 | 0.84255 | 2.41E-58 | -1.69E-46 |
| MS.gene057129 | MS.gene79304 | 0.838256 | 3.20E-57 | -1.69E-46 |
| MS.gene057146 | MS.gene79304 | 0.825489 | 4.56E-54 | -1.69E-46 |
| MS.gene057331 | MS.gene79304 | 0.847216 | 1.33E-59 | -1.69E-46 |
| MS.gene05735 | MS.gene79304 | 0.819109 | 1.38E-52 | -1.69E-46 |
| MS.gene057416 | MS.gene79304 | 0.843675 | 1.21E-58 | -1.69E-46 |
| MS.gene057658 | MS.gene79304 | 0.830505 | 2.83E-55 | -1.69E-46 |
| MS.gene057734 | MS.gene79304 | 0.83263 | 8.47E-56 | -1.69E-46 |
| MS.gene057823 | MS.gene79304 | 0.80047 | 1.42E-48 | -1.69E-46 |
| MS.gene057856 | MS.gene79304 | 0.807185 | 5.72E-50 | -1.69E-46 |
| MS.gene057926 | MS.gene79304 | 0.847319 | 1.25E-59 | -1.69E-46 |
| MS.gene057927 | MS.gene79304 | 0.80289 | 4.53E-49 | -1.69E-46 |
| MS.gene058006 | MS.gene79304 | 0.859131 | 5.11E-63 | -1.69E-46 |
| MS.gene058019 | MS.gene79304 | 0.82753 | 1.49E-54 | -1.69E-46 |
| MS.gene058130 | MS.gene79304 | 0.867915 | 9.62E-66 | -1.69E-46 |
| MS.gene058233 | MS.gene79304 | 0.801041 | 1.09E-48 | -1.69E-46 |
| MS.gene058293 | MS.gene79304 | 0.839171 | 1.86E-57 | -1.69E-46 |
| MS.gene058323 | MS.gene79304 | 0.855312 | 6.86E-62 | -1.69E-46 |
| MS.gene058338 | MS.gene79304 | 0.812476 | 4.16E-51 | -1.69E-46 |
| MS.gene058376 | MS.gene79304 | 0.84146 | 4.69E-58 | -1.69E-46 |
| MS.gene05837 | MS.gene79304 | 0.858895 | 6.02E-63 | -1.69E-46 |
| MS.gene058419 | MS.gene79304 | 0.847598 | 1.05E-59 | -1.69E-46 |
| MS.gene058544 | MS.gene79304 | 0.820678 | 6.04E-53 | -1.69E-46 |
| MS.gene058660 | MS.gene79304 | 0.810679 | 1.02E-50 | -1.69E-46 |
| MS.gene058700 | MS.gene79304 | 0.808638 | 2.81E-50 | -1.69E-46 |
| MS.gene058723 | MS.gene79304 | 0.843318 | 1.51E-58 | -1.69E-46 |
| MS.gene058773 | MS.gene79304 | 0.811851 | 5.69E-51 | -1.69E-46 |
| MS.gene058838 | MS.gene79304 | 0.845813 | 3.21E-59 | -1.69E-46 |
| MS.gene058948 | MS.gene79304 | 0.807321 | 5.35E-50 | -1.69E-46 |
| MS.gene059023 | MS.gene79304 | 0.846458 | 2.14E-59 | -1.69E-46 |
| MS.gene059029 | MS.gene79304 | 0.803851 | 2.86E-49 | -1.69E-46 |
| MS.gene05913 | MS.gene79304 | 0.851694 | 7.51E-61 | -1.69E-46 |
| MS.gene05934 | MS.gene79304 | 0.872068 | 4.22E-67 | -1.69E-46 |
| MS.gene059366 | MS.gene79304 | 0.818318 | 2.09E-52 | -1.69E-46 |
| MS.gene059368 | MS.gene79304 | 0.819049 | 1.42E-52 | -1.69E-46 |
| MS.gene059420 | MS.gene79304 | 0.814502 | 1.49E-51 | -1.69E-46 |
| MS.gene059536 | MS.gene79304 | 0.836375 | 9.71E-57 | -1.69E-46 |
| MS.gene059667 | MS.gene79304 | 0.82947 | 5.06E-55 | -1.69E-46 |
| MS.gene059677 | MS.gene79304 | 0.811191 | 7.92E-51 | -1.69E-46 |
| MS.gene059688 | MS.gene79304 | 0.85514 | 7.71E-62 | -1.69E-46 |
| MS.gene059692 | MS.gene79304 | 0.843222 | 1.60E-58 | -1.69E-46 |
| MS.gene059695 | MS.gene79304 | 0.851285 | 9.81E-61 | -1.69E-46 |
| MS.gene059718 | MS.gene79304 | 0.845344 | 4.31E-59 | -1.69E-46 |
| MS.gene059807 | MS.gene79304 | 0.80715 | 5.82E-50 | -1.69E-46 |
| MS.gene059902 | MS.gene79304 | 0.856329 | 3.46E-62 | -1.69E-46 |
| MS.gene059960 | MS.gene79304 | 0.829693 | 4.46E-55 | -1.69E-46 |
| MS.gene060193 | MS.gene79304 | 0.820766 | 5.77E-53 | -1.69E-46 |
| MS.gene060403 | MS.gene79304 | 0.83383 | 4.26E-56 | -1.69E-46 |
| MS.gene060482 | MS.gene79304 | 0.806739 | 7.11E-50 | -1.69E-46 |
| MS.gene060762 | MS.gene79304 | 0.818973 | 1.48E-52 | -1.69E-46 |
| MS.gene060804 | MS.gene79304 | 0.893036 | 9.13E-75 | -1.69E-46 |
| MS.gene060818 | MS.gene79304 | 0.836608 | 8.47E-57 | -1.69E-46 |
| MS.gene060835 | MS.gene79304 | 0.873004 | 2.06E-67 | -1.69E-46 |
| MS.gene060837 | MS.gene79304 | 0.862929 | 3.58E-64 | -1.69E-46 |
| MS.gene06092 | MS.gene79304 | 0.890929 | 6.32E-74 | -1.69E-46 |
| MS.gene061041 | MS.gene79304 | 0.868846 | 4.82E-66 | -1.69E-46 |
| MS.gene061051 | MS.gene79304 | -0.810388 | 1.18E-50 | -1.69E-46 |
| MS.gene061150 | MS.gene79304 | 0.809048 | 2.29E-50 | -1.69E-46 |
| MS.gene061154 | MS.gene79304 | 0.855837 | 4.83E-62 | -1.69E-46 |
| MS.gene061200 | MS.gene79304 | 0.819671 | 1.03E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79304.t1 | MTR_6g465290 | 95.455 | 242 | 11 | 0 | 1 | 242 | 1 | 242 | 1.78e-168 | 464 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79304.t1 | AT5G62140 | 58.768 | 211 | 79 | 3 | 37 | 239 | 27 | 237 | 1.95e-78 | 236 |
Find 38 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGGTTGATCTTGAGTTTA+AGG | 0.089999 | 6.3:-56550337 | MS.gene79304:intron |
| AGCTCTACAACAAAGTTTCT+TGG | 0.240920 | 6.3:-56551419 | MS.gene79304:CDS |
| TGATGGAGTTTCAATTGTTT+TGG | 0.254521 | 6.3:+56551637 | None:intergenic |
| TTCTGCTGGTAGTATCTATA+AGG | 0.352592 | 6.3:-56550301 | MS.gene79304:CDS |
| GGAGGAATAGGAACTGGTTT+TGG | 0.356740 | 6.3:-56551524 | MS.gene79304:CDS |
| CTCTTATGATGAAGAAGAAA+TGG | 0.372803 | 6.3:+56550077 | None:intergenic |
| GGAAGTATCAATCAAGAATC+TGG | 0.380544 | 6.3:-56551344 | MS.gene79304:CDS |
| GTGCATGCCTTATCATTTGA+TGG | 0.383095 | 6.3:+56551620 | None:intergenic |
| TTCCTAATGAATTCCTTCCT+TGG | 0.410690 | 6.3:-56550141 | MS.gene79304:CDS |
| TATGATGCTGAAGGAGGAAT+AGG | 0.412723 | 6.3:-56551536 | MS.gene79304:CDS |
| GACCAAGGAAGGAATTCATT+AGG | 0.428876 | 6.3:+56550139 | None:intergenic |
| TCAGCATCATACTCAAAATC+AGG | 0.441137 | 6.3:+56551548 | None:intergenic |
| ATATGAAATTTGCATTGTAT+TGG | 0.443194 | 6.3:+56551740 | None:intergenic |
| TGAAATTTGCATTGTATTGG+TGG | 0.454546 | 6.3:+56551743 | None:intergenic |
| GAGTGGAAGAGGCATGAGAT+TGG | 0.458016 | 6.3:-56550220 | MS.gene79304:CDS |
| GTTGTAGAGCTTGTTAATGA+TGG | 0.460648 | 6.3:+56551431 | None:intergenic |
| TGAAACTCCATCAAATGATA+AGG | 0.461780 | 6.3:-56551627 | MS.gene79304:CDS |
| AGGCATGAGATTGGATGAAG+AGG | 0.462908 | 6.3:-56550211 | MS.gene79304:CDS |
| TTCATTAGGAAATCATCAAT+AGG | 0.476929 | 6.3:+56550153 | None:intergenic |
| ATACTTCCTTGAAAAGCTTG+TGG | 0.499885 | 6.3:+56551359 | None:intergenic |
| ACCAGCAGAAAACAAGAACT+TGG | 0.514821 | 6.3:+56550314 | None:intergenic |
| GCTGAAGGAGGAATAGGAAC+TGG | 0.519626 | 6.3:-56551530 | MS.gene79304:CDS |
| GGCATGAGATTGGATGAAGA+GGG | 0.522269 | 6.3:-56550210 | MS.gene79304:CDS |
| TTGACATCTGAAGAATCAAA+GGG | 0.535919 | 6.3:-56550252 | MS.gene79304:CDS |
| GGGACTATGAAGAGTGGAAG+AGG | 0.554975 | 6.3:-56550231 | MS.gene79304:CDS |
| GTTGACATCTGAAGAATCAA+AGG | 0.561822 | 6.3:-56550253 | MS.gene79304:CDS |
| CTCAGTAGGTAGACCAAGGA+AGG | 0.566579 | 6.3:+56550128 | None:intergenic |
| AACACTGTCTTCACAAGTAG+TGG | 0.578508 | 6.3:+56550273 | None:intergenic |
| AGTTCAGCAAGACACTCAGT+AGG | 0.604330 | 6.3:+56550114 | None:intergenic |
| TGAAGAGGGAAAGTGCAGAC+TGG | 0.610863 | 6.3:-56550196 | MS.gene79304:CDS |
| TTTGAGTATGATGCTGAAGG+AGG | 0.619744 | 6.3:-56551542 | MS.gene79304:CDS |
| GAGGGAAAGTGCAGACTGGT+TGG | 0.627517 | 6.3:-56550192 | MS.gene79304:CDS |
| TCAAAGGGGACTATGAAGAG+TGG | 0.635845 | 6.3:-56550237 | MS.gene79304:CDS |
| GACACTCAGTAGGTAGACCA+AGG | 0.644039 | 6.3:+56550124 | None:intergenic |
| ACTGTCTTCACAAGTAGTGG+TGG | 0.644274 | 6.3:+56550276 | None:intergenic |
| TATCAATCAAGAATCTGGCA+AGG | 0.648241 | 6.3:-56551339 | MS.gene79304:intron |
| AAAGTGCAGACTGGTTGGTG+TGG | 0.674945 | 6.3:-56550187 | MS.gene79304:CDS |
| TGACATCTGAAGAATCAAAG+GGG | 0.752025 | 6.3:-56550251 | MS.gene79304:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAAAAAAAAGTAAGTTTTA+CGG | + | chr6.3:56550765-56550784 | None:intergenic | 10.0% |
| !!! | ATTTTTATTTTTTTCTTACA+AGG | - | chr6.3:56551495-56551514 | MS.gene79304:CDS | 10.0% |
| !!! | CTATTTTAATATTATATACT+AGG | + | chr6.3:56551110-56551129 | None:intergenic | 10.0% |
| !!! | TTATTTTCTCAAAATTTTAC+TGG | - | chr6.3:56551403-56551422 | MS.gene79304:CDS | 15.0% |
| !! | ACAATATCAATCTTCAAAAA+TGG | + | chr6.3:56550470-56550489 | None:intergenic | 20.0% |
| !! | ATATGAAATTTGCATTGTAT+TGG | + | chr6.3:56550113-56550132 | None:intergenic | 20.0% |
| !! | TACAATCTTTATAGCTTATA+TGG | + | chr6.3:56550541-56550560 | None:intergenic | 20.0% |
| !! | TTCAATATAATCACATATGT+CGG | + | chr6.3:56551378-56551397 | None:intergenic | 20.0% |
| !!! | AACATTTTCCAAAAAATCTA+AGG | + | chr6.3:56550641-56550660 | None:intergenic | 20.0% |
| !!! | ACATTTTCCAAAAAATCTAA+GGG | + | chr6.3:56550640-56550659 | None:intergenic | 20.0% |
| ! | AAAATGTTAACTAGTACTCT+AGG | - | chr6.3:56550653-56550672 | MS.gene79304:intron | 25.0% |
| ! | AAATGTTAACTAGTACTCTA+GGG | - | chr6.3:56550654-56550673 | MS.gene79304:intron | 25.0% |
| ! | AATGCAATCATATCTCAATT+TGG | + | chr6.3:56550846-56550865 | None:intergenic | 25.0% |
| ! | ACAAACTCTTATCATTAAAC+TGG | + | chr6.3:56550934-56550953 | None:intergenic | 25.0% |
| ! | ACTATATTTGTTTCCTTAAC+TGG | + | chr6.3:56550683-56550702 | None:intergenic | 25.0% |
| ! | ATATCAATCTTCAAAAATGG+TGG | + | chr6.3:56550467-56550486 | None:intergenic | 25.0% |
| ! | CTTGAAGGAATAAACATTTA+GGG | - | chr6.3:56551244-56551263 | MS.gene79304:intron | 25.0% |
| ! | TCTTGAAGGAATAAACATTT+AGG | - | chr6.3:56551243-56551262 | MS.gene79304:intron | 25.0% |
| ! | TTCATTAGGAAATCATCAAT+AGG | + | chr6.3:56551700-56551719 | None:intergenic | 25.0% |
| ! | TTTGTTAACTAACTGACTAA+CGG | - | chr6.3:56551166-56551185 | MS.gene79304:intron | 25.0% |
| !!! | TATGTATCCCTTAGATTTTT+TGG | - | chr6.3:56550630-56550649 | MS.gene79304:intron | 25.0% |
| !!! | TTGTTTTGTTTTTCACTTGA+GGG | + | chr6.3:56550185-56550204 | None:intergenic | 25.0% |
| !!! | TTTGTTTTGTTTTTCACTTG+AGG | + | chr6.3:56550186-56550205 | None:intergenic | 25.0% |
| AAACCATTAACTATGAAGCA+CGG | + | chr6.3:56551471-56551490 | None:intergenic | 30.0% | |
| GACATTCAATGACTAAACTA+AGG | - | chr6.3:56551193-56551212 | MS.gene79304:intron | 30.0% | |
| GATCCTGTAATATTAGAAGA+GGG | - | chr6.3:56551008-56551027 | MS.gene79304:intron | 30.0% | |
| TACCATTGATACCAAAAACT+TGG | + | chr6.3:56550981-56551000 | None:intergenic | 30.0% | |
| TGAAACTCCATCAAATGATA+AGG | - | chr6.3:56550223-56550242 | MS.gene79304:CDS | 30.0% | |
| TGAAATTTGCATTGTATTGG+TGG | + | chr6.3:56550110-56550129 | None:intergenic | 30.0% | |
| TGATCCTGTAATATTAGAAG+AGG | - | chr6.3:56551007-56551026 | MS.gene79304:intron | 30.0% | |
| TTGACATCTGAAGAATCAAA+GGG | - | chr6.3:56551598-56551617 | MS.gene79304:CDS | 30.0% | |
| ! | TGATGGAGTTTCAATTGTTT+TGG | + | chr6.3:56550216-56550235 | None:intergenic | 30.0% |
| ACTCCCTCTTCTAATATTAC+AGG | + | chr6.3:56551014-56551033 | None:intergenic | 35.0% | |
| AGCTCTACAACAAAGTTTCT+TGG | - | chr6.3:56550431-56550450 | MS.gene79304:intron | 35.0% | |
| AGTCTAAATCCTCGAGTTAT+TGG | - | chr6.3:56551031-56551050 | MS.gene79304:intron | 35.0% | |
| ATACTTCCTTGAAAAGCTTG+TGG | + | chr6.3:56550494-56550513 | None:intergenic | 35.0% | |
| ATTTCACAACCAATAACTCG+AGG | + | chr6.3:56551043-56551062 | None:intergenic | 35.0% | |
| CAACATAGTCCAGTAACAAT+TGG | + | chr6.3:56551298-56551317 | None:intergenic | 35.0% | |
| GAGTCAAATCCAATTGTTAC+TGG | - | chr6.3:56551286-56551305 | MS.gene79304:intron | 35.0% | |
| GCAATCATATCTCAATTTGG+TGG | + | chr6.3:56550843-56550862 | None:intergenic | 35.0% | |
| GTTGACATCTGAAGAATCAA+AGG | - | chr6.3:56551597-56551616 | MS.gene79304:CDS | 35.0% | |
| TATCAATCAAGAATCTGGCA+AGG | - | chr6.3:56550511-56550530 | MS.gene79304:intron | 35.0% | |
| TCAGCATCATACTCAAAATC+AGG | + | chr6.3:56550305-56550324 | None:intergenic | 35.0% | |
| TCTAAGGGATACATATAGCT+AGG | + | chr6.3:56550625-56550644 | None:intergenic | 35.0% | |
| TGACATCTGAAGAATCAAAG+GGG | - | chr6.3:56551599-56551618 | MS.gene79304:CDS | 35.0% | |
| TGACTAAACTAAGGAACGAA+AGG | - | chr6.3:56551202-56551221 | MS.gene79304:intron | 35.0% | |
| TTCCTAATGAATTCCTTCCT+TGG | - | chr6.3:56551709-56551728 | MS.gene79304:CDS | 35.0% | |
| TTCTGCTGGTAGTATCTATA+AGG | - | chr6.3:56551549-56551568 | MS.gene79304:CDS | 35.0% | |
| TTTGAAGACTGAACTCTTGA+AGG | - | chr6.3:56551229-56551248 | MS.gene79304:intron | 35.0% | |
| ! | AAAGACTAAGTGCCACTAAA+AGG | + | chr6.3:56550596-56550615 | None:intergenic | 35.0% |
| ! | AGAGAAAGAGTCCAAGTTTT+TGG | - | chr6.3:56550967-56550986 | MS.gene79304:intron | 35.0% |
| ! | ATTGTTCCACAAGCTTTTCA+AGG | - | chr6.3:56550485-56550504 | MS.gene79304:intron | 35.0% |
| ! | CAAGGTTGATCTTGAGTTTA+AGG | - | chr6.3:56551513-56551532 | MS.gene79304:CDS | 35.0% |
| ! | CTCTAATTGATAGTATGTCC+TGG | - | chr6.3:56551327-56551346 | MS.gene79304:intron | 35.0% |
| ! | TATGTGTCTACACCTTTTAG+TGG | - | chr6.3:56550581-56550600 | MS.gene79304:intron | 35.0% |
| !! | GATTTTGAGTATGATGCTGA+AGG | - | chr6.3:56550305-56550324 | MS.gene79304:CDS | 35.0% |
| !! | GGAAGTATCAATCAAGAATC+TGG | - | chr6.3:56550506-56550525 | MS.gene79304:intron | 35.0% |
| !! | GTTGTAGAGCTTGTTAATGA+TGG | + | chr6.3:56550422-56550441 | None:intergenic | 35.0% |
| !!! | GTCCAAGTTTTTGGTATCAA+TGG | - | chr6.3:56550976-56550995 | MS.gene79304:intron | 35.0% |
| AACACTGTCTTCACAAGTAG+TGG | + | chr6.3:56551580-56551599 | None:intergenic | 40.0% | |
| ACCAGCAGAAAACAAGAACT+TGG | + | chr6.3:56551539-56551558 | None:intergenic | 40.0% | |
| CTCAGAGAATACTAGTTAGC+AGG | - | chr6.3:56550798-56550817 | MS.gene79304:intron | 40.0% | |
| GACCAAGGAAGGAATTCATT+AGG | + | chr6.3:56551714-56551733 | None:intergenic | 40.0% | |
| GTGCATGCCTTATCATTTGA+TGG | + | chr6.3:56550233-56550252 | None:intergenic | 40.0% | |
| TATGATGCTGAAGGAGGAAT+AGG | - | chr6.3:56550314-56550333 | MS.gene79304:CDS | 40.0% | |
| TGTCCGTGCTTCATAGTTAA+TGG | - | chr6.3:56551465-56551484 | MS.gene79304:CDS | 40.0% | |
| TTTGAGTATGATGCTGAAGG+AGG | - | chr6.3:56550308-56550327 | MS.gene79304:CDS | 40.0% | |
| !! | ACAAAAAGTGCTTGCAAGCT+TGG | - | chr6.3:56550266-56550285 | MS.gene79304:CDS | 40.0% |
| !! | TACTCTAGGGTTACCAGTTA+AGG | - | chr6.3:56550667-56550686 | MS.gene79304:intron | 40.0% |
| ACTGTCTTCACAAGTAGTGG+TGG | + | chr6.3:56551577-56551596 | None:intergenic | 45.0% | |
| AGGCATGAGATTGGATGAAG+AGG | - | chr6.3:56551639-56551658 | MS.gene79304:CDS | 45.0% | |
| AGTTCAGCAAGACACTCAGT+AGG | + | chr6.3:56551739-56551758 | None:intergenic | 45.0% | |
| GGCATGAGATTGGATGAAGA+GGG | - | chr6.3:56551640-56551659 | MS.gene79304:CDS | 45.0% | |
| TCAAAGGGGACTATGAAGAG+TGG | - | chr6.3:56551613-56551632 | MS.gene79304:CDS | 45.0% | |
| !! | GGAGGAATAGGAACTGGTTT+TGG | - | chr6.3:56550326-56550345 | MS.gene79304:CDS | 45.0% |
| !!! | GCCAAGTTCTTGTTTTCTGC+TGG | - | chr6.3:56551535-56551554 | MS.gene79304:CDS | 45.0% |
| ACCGGAACACGACACTGATA+CGG | + | chr6.3:56551440-56551459 | None:intergenic | 50.0% | |
| CTCAGTAGGTAGACCAAGGA+AGG | + | chr6.3:56551725-56551744 | None:intergenic | 50.0% | |
| GACACTCAGTAGGTAGACCA+AGG | + | chr6.3:56551729-56551748 | None:intergenic | 50.0% | |
| GCTGAAGGAGGAATAGGAAC+TGG | - | chr6.3:56550320-56550339 | MS.gene79304:CDS | 50.0% | |
| GGGACTATGAAGAGTGGAAG+AGG | - | chr6.3:56551619-56551638 | MS.gene79304:CDS | 50.0% | |
| GTGTCTGACATGTGTCGTGT+TGG | - | chr6.3:56551349-56551368 | MS.gene79304:CDS | 50.0% | |
| TCCGTATCAGTGTCGTGTTC+CGG | - | chr6.3:56551436-56551455 | MS.gene79304:CDS | 50.0% | |
| ! | AAAGTGCAGACTGGTTGGTG+TGG | - | chr6.3:56551663-56551682 | MS.gene79304:CDS | 50.0% |
| ! | GAGTGGAAGAGGCATGAGAT+TGG | - | chr6.3:56551630-56551649 | MS.gene79304:CDS | 50.0% |
| ! | TGAAGAGGGAAAGTGCAGAC+TGG | - | chr6.3:56551654-56551673 | MS.gene79304:CDS | 50.0% |
| CACGACACATGTCAGACACC+AGG | + | chr6.3:56551348-56551367 | None:intergenic | 55.0% | |
| TGAAGCACGGACACAGACAC+CGG | + | chr6.3:56551458-56551477 | None:intergenic | 55.0% | |
| ! | GAGGGAAAGTGCAGACTGGT+TGG | - | chr6.3:56551658-56551677 | MS.gene79304:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 56550080 | 56551792 | 56550080 | ID=MS.gene79304 |
| chr6.3 | mRNA | 56550080 | 56551792 | 56550080 | ID=MS.gene79304.t1;Parent=MS.gene79304 |
| chr6.3 | exon | 56551340 | 56551792 | 56551340 | ID=MS.gene79304.t1.exon1;Parent=MS.gene79304.t1 |
| chr6.3 | CDS | 56551340 | 56551792 | 56551340 | ID=cds.MS.gene79304.t1;Parent=MS.gene79304.t1 |
| chr6.3 | exon | 56550080 | 56550355 | 56550080 | ID=MS.gene79304.t1.exon2;Parent=MS.gene79304.t1 |
| chr6.3 | CDS | 56550080 | 56550355 | 56550080 | ID=cds.MS.gene79304.t1;Parent=MS.gene79304.t1 |
| Gene Sequence |
| Protein sequence |
