AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1439930


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1439930 MtrunA17_Chr4g0067451 96.694 121 4 0 1 121 1 121 6.71e-78 225
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1439930 AT3G06890.1 75.591 127 25 1 1 121 1 127 1.55e-31 108

Find 33 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACCACCTTGTTGTCTTTC+TGG 0.014264 tig0028916:-3655 None:intergenic
GTAATAGTTCCTTTCCTTCT+CGG 0.355214 tig0028916:+3724 Msa1439930:CDS
AGGTTCACCGGTGGAGAAAA+TGG 0.355541 tig0028916:-3875 None:intergenic
TGGAACCGTGTTTCGATCTT+CGG 0.370599 tig0028916:+3970 Msa1439930:CDS
GATACTATTCATCGTGTTAG+TGG 0.389930 tig0028916:+3898 Msa1439930:CDS
CCTTTCCTTCTCGGAGATCA+AGG 0.391728 tig0028916:+3733 Msa1439930:CDS
AGCTATCACAGAAGCTATAT+CGG 0.433454 tig0028916:+3759 Msa1439930:CDS
CTAACACGATGAATAGTATC+AGG 0.440753 tig0028916:-3895 None:intergenic
AGCAGTGGTGGTAGCTATAG+TGG 0.468105 tig0028916:+3702 Msa1439930:CDS
ATCGTGAGAAGAAGGATGAT+TGG 0.488963 tig0028916:-3814 None:intergenic
GGAACTGAATCGTGAGAAGA+AGG 0.507982 tig0028916:-3822 None:intergenic
GAAAGACAACAAGGTGGTGG+AGG 0.540517 tig0028916:+3658 Msa1439930:CDS
CGTGTTAGTGGATCACCGTT+CGG 0.558827 tig0028916:+3910 Msa1439930:CDS
CCTTGATCTCCGAGAAGGAA+AGG 0.562958 tig0028916:-3733 None:intergenic
CCAGAAAGACAACAAGGTGG+TGG 0.583317 tig0028916:+3655 Msa1439930:CDS
ATGAATAGTATCAGGTTCAC+CGG 0.583799 tig0028916:-3887 None:intergenic
TCGGAGCTGCTAAGTCCACT+TGG 0.597655 tig0028916:+3778 Msa1439930:CDS
AACCGTGTTTCGATCTTCGG+TGG 0.599415 tig0028916:+3973 Msa1439930:CDS
CGGTATTCTTCTAGCAGTGG+TGG 0.606734 tig0028916:+3690 Msa1439930:CDS
CTTCGCCTTGATCTCCGAGA+AGG 0.615486 tig0028916:-3738 None:intergenic
AGGAAAAGAGCAACACCGAA+CGG 0.621281 tig0028916:-3925 None:intergenic
CAGTTCCTCTCATCTGAACG+TGG 0.622156 tig0028916:+3838 Msa1439930:CDS
AAAGCACAAGAATGAGAACA+AGG 0.624793 tig0028916:-3945 None:intergenic
ACGATCCACGTTCAGATGAG+AGG 0.629269 tig0028916:-3843 None:intergenic
ACACGGTATTCTTCTAGCAG+TGG 0.660684 tig0028916:+3687 Msa1439930:CDS
GGTAGAAGAAGAAGACCAAG+TGG 0.662368 tig0028916:-3793 None:intergenic
AATAGTATCAGGTTCACCGG+TGG 0.662862 tig0028916:-3884 None:intergenic
ATGTATCCAGAAAGACAACA+AGG 0.678373 tig0028916:+3649 Msa1439930:CDS
CACCACCGAAGATCGAAACA+CGG 0.699903 tig0028916:-3975 None:intergenic
GGTGGTGGAGGAGCACCACA+CGG 0.704308 tig0028916:+3670 Msa1439930:CDS
TTCACCGGTGGAGAAAATGG+CGG 0.726368 tig0028916:-3872 None:intergenic
TATCCAGAAAGACAACAAGG+TGG 0.796650 tig0028916:+3652 Msa1439930:CDS
ACTGCTAGAAGAATACCGTG+TGG 0.809004 tig0028916:-3685 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGTATCCAGAAAGACAACA+AGG + tig0028916:3649-3668 Msa1439930:CDS 35.0%
GTAATAGTTCCTTTCCTTCT+CGG + tig0028916:3724-3743 Msa1439930:CDS 35.0%
AGCTATCACAGAAGCTATAT+CGG + tig0028916:3759-3778 Msa1439930:CDS 35.0%
ATGAATAGTATCAGGTTCAC+CGG - tig0028916:3890-3909 None:intergenic 35.0%
CTAACACGATGAATAGTATC+AGG - tig0028916:3898-3917 None:intergenic 35.0%
GATACTATTCATCGTGTTAG+TGG + tig0028916:3898-3917 Msa1439930:CDS 35.0%
AAAGCACAAGAATGAGAACA+AGG - tig0028916:3948-3967 None:intergenic 35.0%
! TTCTCATTCTTGTGCTTTTG+TGG + tig0028916:3950-3969 Msa1439930:CDS 35.0%
TATCCAGAAAGACAACAAGG+TGG + tig0028916:3652-3671 Msa1439930:CDS 40.0%
ATCGTGAGAAGAAGGATGAT+TGG - tig0028916:3817-3836 None:intergenic 40.0%
ACTGCTAGAAGAATACCGTG+TGG - tig0028916:3688-3707 None:intergenic 45.0%
ACACGGTATTCTTCTAGCAG+TGG + tig0028916:3687-3706 Msa1439930:CDS 45.0%
GGTAGAAGAAGAAGACCAAG+TGG - tig0028916:3796-3815 None:intergenic 45.0%
GGAACTGAATCGTGAGAAGA+AGG - tig0028916:3825-3844 None:intergenic 45.0%
AATAGTATCAGGTTCACCGG+TGG - tig0028916:3887-3906 None:intergenic 45.0%
AGGAAAAGAGCAACACCGAA+CGG - tig0028916:3928-3947 None:intergenic 45.0%
TGGAACCGTGTTTCGATCTT+CGG + tig0028916:3970-3989 Msa1439930:CDS 45.0%
! CCACCACCTTGTTGTCTTTC+TGG - tig0028916:3658-3677 None:intergenic 50.0%
CCAGAAAGACAACAAGGTGG+TGG + tig0028916:3655-3674 Msa1439930:CDS 50.0%
! GAAAGACAACAAGGTGGTGG+AGG + tig0028916:3658-3677 Msa1439930:CDS 50.0%
CGGTATTCTTCTAGCAGTGG+TGG + tig0028916:3690-3709 Msa1439930:CDS 50.0%
AGCAGTGGTGGTAGCTATAG+TGG + tig0028916:3702-3721 Msa1439930:CDS 50.0%
CCTTGATCTCCGAGAAGGAA+AGG - tig0028916:3736-3755 None:intergenic 50.0%
CCTTTCCTTCTCGGAGATCA+AGG + tig0028916:3733-3752 Msa1439930:CDS 50.0%
CAGTTCCTCTCATCTGAACG+TGG + tig0028916:3838-3857 Msa1439930:CDS 50.0%
ACGATCCACGTTCAGATGAG+AGG - tig0028916:3846-3865 None:intergenic 50.0%
!! ATCTCCGCCATTTTCTCCAC+CGG + tig0028916:3868-3887 Msa1439930:CDS 50.0%
TTCACCGGTGGAGAAAATGG+CGG - tig0028916:3875-3894 None:intergenic 50.0%
AGGTTCACCGGTGGAGAAAA+TGG - tig0028916:3878-3897 None:intergenic 50.0%
! CGTGTTAGTGGATCACCGTT+CGG + tig0028916:3910-3929 Msa1439930:CDS 50.0%
AACCGTGTTTCGATCTTCGG+TGG + tig0028916:3973-3992 Msa1439930:CDS 50.0%
CACCACCGAAGATCGAAACA+CGG - tig0028916:3978-3997 None:intergenic 50.0%
CTTCGCCTTGATCTCCGAGA+AGG - tig0028916:3741-3760 None:intergenic 55.0%
TCGGAGCTGCTAAGTCCACT+TGG + tig0028916:3778-3797 Msa1439930:CDS 55.0%
!! GGTGGTGGAGGAGCACCACA+CGG + tig0028916:3670-3689 Msa1439930:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0028916 gene 3649 4014 3649 ID=Msa1439930;Name=Msa1439930
tig0028916 mRNA 3649 4014 3649 ID=Msa1439930-mRNA-1;Parent=Msa1439930;Name=Msa1439930-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|121
tig0028916 exon 3649 4014 3649 ID=Msa1439930-mRNA-1:exon:12000;Parent=Msa1439930-mRNA-1
tig0028916 CDS 3649 4014 3649 ID=Msa1439930-mRNA-1:cds;Parent=Msa1439930-mRNA-1
Gene Sequence

>Msa1439930

ATGTATCCAGAAAGACAACAAGGTGGTGGAGGAGCACCACACGGTATTCTTCTAGCAGTGGTGGTAGCTATAGTGGTAATAGTTCCTTTCCTTCTCGGAGATCAAGGCGAAGCTATCACAGAAGCTATATCGGAGCTGCTAAGTCCACTTGGTCTTCTTCTTCTACCAATCATCCTTCTTCTCACGATTCAGTTCCTCTCATCTGAACGTGGATCGTTCATCTCCGCCATTTTCTCCACCGGTGAACCTGATACTATTCATCGTGTTAGTGGATCACCGTTCGGTGTTGCTCTTTTCCTTGTTCTCATTCTTGTGCTTTTGTGGAACCGTGTTTCGATCTTCGGTGGTGATGATTCAGATGACTGA

Protein sequence

>Msa1439930

MYPERQQGGGGAPHGILLAVVVAIVVIVPFLLGDQGEAITEAISELLSPLGLLLLPIILLLTIQFLSSERGSFISAIFSTGEPDTIHRVSGSPFGVALFLVLILVLLWNRVSIFGGDDSDD*