AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33658


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33658.t1 MTR_3g089065 98.286 175 3 0 1 175 1 175 7.82e-127 353
MS.gene33658.t1 MTR_2g079420 69.167 120 36 1 55 173 54 173 9.55e-62 189
MS.gene33658.t1 MTR_8g059015 63.077 130 48 0 44 173 47 176 5.35e-61 187
MS.gene33658.t1 MTR_4g085880 47.573 103 54 0 73 175 68 170 2.76e-34 119
MS.gene33658.t1 MTR_2g079340 52.475 101 48 0 73 173 39 139 7.53e-34 117
MS.gene33658.t1 MTR_2g079400 47.222 108 57 0 64 171 28 135 3.21e-32 112
MS.gene33658.t1 MTR_2g079360 39.568 139 81 2 35 170 9 147 2.39e-30 108
MS.gene33658.t1 MTR_1g114290 39.640 111 55 3 70 175 70 173 1.80e-24 94.4
MS.gene33658.t1 MTR_2g079380 47.778 90 47 0 64 153 12 101 1.08e-23 94.4
MS.gene33658.t1 MTR_1g098660 38.144 97 60 0 74 170 66 162 5.05e-22 87.4
MS.gene33658.t1 MTR_5g021180 39.252 107 60 3 70 173 11 115 5.41e-20 80.9
MS.gene33658.t1 MTR_1g023140 35.780 109 65 3 70 175 9 115 1.33e-17 74.7
MS.gene33658.t1 MTR_7g009070 36.905 84 51 2 89 172 51 132 3.76e-17 73.9
MS.gene33658.t1 MTR_3g112410 34.513 113 69 3 63 172 2 112 5.45e-17 73.2
MS.gene33658.t1 MTR_4g094690 36.275 102 52 4 61 159 64 155 1.43e-15 70.9
MS.gene33658.t1 MTR_7g093490 27.564 156 95 4 15 159 21 169 6.67e-14 66.6
MS.gene33658.t1 MTR_8g107460 38.750 80 47 2 93 172 283 360 7.86e-14 68.6
MS.gene33658.t1 MTR_1g037680 32.558 86 56 1 88 171 99 184 3.00e-12 62.4
MS.gene33658.t1 MTR_1g087520 34.831 89 56 1 66 152 145 233 3.15e-12 63.9
MS.gene33658.t1 MTR_5g084930 36.709 79 50 0 73 151 33 111 5.50e-12 63.5
MS.gene33658.t1 MTR_7g073680 33.803 71 46 1 89 159 49 118 5.52e-11 57.8
MS.gene33658.t1 MTR_7g093490 25.806 155 93 4 15 159 21 163 7.76e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33658.t1 AT3G15360 58.197 122 48 1 55 173 70 191 8.05e-51 162
MS.gene33658.t1 AT1G03680 47.020 151 80 0 23 173 29 179 4.26e-50 159
MS.gene33658.t1 AT4G03520 51.639 122 59 0 52 173 64 185 2.79e-49 157
MS.gene33658.t1 AT2G15570 52.427 103 49 0 73 175 71 173 1.01e-37 128
MS.gene33658.t1 AT2G15570 52.427 103 49 0 73 175 72 174 1.04e-37 128
MS.gene33658.t1 AT4G03520 54.930 71 32 0 103 173 1 71 9.63e-27 97.1
MS.gene33658.t1 AT1G76760 35.606 132 79 2 39 170 40 165 3.60e-23 90.5
MS.gene33658.t1 AT1G43560 39.175 97 59 0 74 170 64 160 7.11e-23 89.7
MS.gene33658.t1 AT1G50320 31.852 135 78 4 46 175 49 174 2.56e-19 80.9
MS.gene33658.t1 AT3G51030 36.842 114 67 3 63 173 2 113 1.02e-18 77.4
MS.gene33658.t1 AT1G19730 34.513 113 70 2 63 172 2 113 1.35e-18 77.4
MS.gene33658.t1 AT5G42980 37.500 88 53 2 85 172 26 111 6.37e-17 73.2
MS.gene33658.t1 AT2G35010 34.568 162 88 7 8 159 27 180 1.15e-16 74.3
MS.gene33658.t1 AT2G35010 34.568 162 88 7 8 159 27 180 1.15e-16 74.3
MS.gene33658.t1 AT4G12170 36.538 104 65 1 70 173 51 153 2.65e-16 72.4
MS.gene33658.t1 AT1G52990 42.391 92 51 2 83 173 223 313 8.48e-16 73.6
MS.gene33658.t1 AT5G39950 39.759 83 48 2 89 171 50 130 1.64e-15 69.7
MS.gene33658.t1 AT1G45145 35.294 85 51 2 78 159 16 99 6.90e-14 65.1
MS.gene33658.t1 AT1G31020 41.096 73 40 2 88 158 73 144 1.04e-13 65.5
MS.gene33658.t1 AT3G54960 30.097 103 71 1 69 171 100 201 1.13e-11 62.8
MS.gene33658.t1 AT3G54960 30.097 103 71 1 69 171 100 201 1.19e-11 62.4
MS.gene33658.t1 AT1G21750 37.975 79 47 1 70 146 376 454 2.63e-11 61.6
MS.gene33658.t1 AT1G21750 37.975 79 47 1 70 146 376 454 3.22e-11 61.2
MS.gene33658.t1 AT1G59730 30.108 93 63 2 80 172 37 127 3.60e-11 58.2
MS.gene33658.t1 AT2G32920 36.111 72 46 0 76 147 170 241 3.89e-11 60.8
MS.gene33658.t1 AT3G06730 29.885 87 59 1 86 170 94 180 4.08e-11 59.3
MS.gene33658.t1 AT5G16400 36.538 104 53 5 65 159 71 170 9.75e-11 58.2

Find 26 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCTAGCTCATCAATAAT+AGG 0.304078 3.4:-82365872 None:intergenic
CCGTACTTTGTTGCTATGTT+TGG 0.325721 3.4:-82365944 None:intergenic
CTCCATTGTTTCCAACTTCA+AGG 0.326814 3.4:+82364735 MS.gene33658:CDS
GTTTCCAACTTCAAGGGAAA+AGG 0.418825 3.4:+82364742 MS.gene33658:CDS
ACAAAGTAGCAACATGGCTT+TGG 0.442578 3.4:+82364670 None:intergenic
TGGAGGAAGACACTGAGGTC+TGG 0.484266 3.4:-82364717 None:intergenic
CACCATGGTGTGGTCCATGC+AGG 0.487978 3.4:+82365840 MS.gene33658:CDS
TTTCTGTAGGCAACACCAGA+TGG 0.513750 3.4:-82364827 None:intergenic
TGCAAGTGAAACCCCTGTGC+TGG 0.515943 3.4:+82365805 MS.gene33658:CDS
GAGCAGTGACTGACTCAAGC+TGG 0.516226 3.4:+82365768 MS.gene33658:CDS
TCCCTTGAAGTTGGAAACAA+TGG 0.517273 3.4:-82364737 None:intergenic
GTGTTCTCAGCTCAAAGAGC+AGG 0.522900 3.4:+82364767 MS.gene33658:CDS
TCCATTGTTTCCAACTTCAA+GGG 0.526913 3.4:+82364736 MS.gene33658:CDS
GAGCTAGCCAAAGAATATGC+TGG 0.527432 3.4:+82365884 MS.gene33658:CDS
TCAACACTATCATTCCCATC+TGG 0.532444 3.4:+82364812 MS.gene33658:CDS
GTTGTAGACAGAGTAGACTT+TGG 0.532653 3.4:-82366037 None:intergenic
CCAAACATAGCAACAAAGTA+CGG 0.542679 3.4:+82365944 MS.gene33658:CDS
GCTCGTGAAGCTGTAAATGA+AGG 0.553122 3.4:+82364869 MS.gene33658:CDS
GTCTACTCTGTCTACAACTG+TGG 0.556776 3.4:+82366042 MS.gene33658:CDS
GGGTTTCACTTGCAAGAACA+AGG 0.564621 3.4:-82365796 None:intergenic
AAGACACTGAGGTCTGGCTG+TGG 0.576209 3.4:-82364711 None:intergenic
TTCTGTAGGCAACACCAGAT+GGG 0.579739 3.4:-82364826 None:intergenic
CTTGAAGTTGGAAACAATGG+AGG 0.602873 3.4:-82364734 None:intergenic
ATAGGCGCTATCATCCTGCA+TGG 0.617910 3.4:-82365854 None:intergenic
ATCCTGCATGGACCACACCA+TGG 0.683330 3.4:-82365842 None:intergenic
AACAATGGAGGAAGACACTG+AGG 0.723169 3.4:-82364722 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTCAGAATACATTACA+AGG - chr3.4:82365474-82365493 None:intergenic 20.0%
!! AAAGACTCATAACAAAATAT+AGG - chr3.4:82365011-82365030 None:intergenic 20.0%
!! TCAATTATAATGTGAAGATA+AGG - chr3.4:82365323-82365342 None:intergenic 20.0%
!!! GAGCTTATTTAAGATATTTT+CGG + chr3.4:82365141-82365160 MS.gene33658:intron 20.0%
!!! TGAATTTTATTTTCTCATTG+AGG + chr3.4:82365486-82365505 MS.gene33658:intron 20.0%
! TTCTTGAAAAATAGAACAGT+TGG - chr3.4:82365983-82366002 None:intergenic 25.0%
!! AATAAAACGAGATTTTCTGT+AGG - chr3.4:82364843-82364862 None:intergenic 25.0%
!! AATTTTTCCAGCATATTCTT+TGG - chr3.4:82365894-82365913 None:intergenic 25.0%
!! CAAATTAACTTTTGCATGTT+TGG - chr3.4:82365677-82365696 None:intergenic 25.0%
!! GAGAAAAAAGAAAGTGTAAT+TGG + chr3.4:82366007-82366026 MS.gene33658:CDS 25.0%
!!! ACTGTTCTATTTTTCAAGAA+TGG + chr3.4:82365983-82366002 MS.gene33658:CDS 25.0%
!!! CATTAAGTTTTAACCTGTTT+AGG - chr3.4:82365382-82365401 None:intergenic 25.0%
CACTATAGTGAATAAACCTA+AGG - chr3.4:82365700-82365719 None:intergenic 30.0%
TGAAACCTTAAGATAGTTTG+TGG + chr3.4:82365286-82365305 MS.gene33658:intron 30.0%
TTCAAGAAAAACAGTTCACA+AGG - chr3.4:82364909-82364928 None:intergenic 30.0%
TTTAGTCATCACTTACAAGA+TGG - chr3.4:82365432-82365451 None:intergenic 30.0%
! ACATTTTTCGTCTTACAAGT+TGG + chr3.4:82365073-82365092 MS.gene33658:intron 30.0%
! GGTTATAAACACTCATTTTC+AGG - chr3.4:82365048-82365067 None:intergenic 30.0%
! TAAATGCATGTATGGATTGA+CGG + chr3.4:82365544-82365563 MS.gene33658:intron 30.0%
!! TGCAAAAGTTAATTTGCCTT+AGG + chr3.4:82365681-82365700 MS.gene33658:intron 30.0%
AACACATACATGCTCAAACT+TGG + chr3.4:82365739-82365758 MS.gene33658:intron 35.0%
AACTTAATTTCCACAGCTAG+GGG + chr3.4:82365342-82365361 MS.gene33658:intron 35.0%
AGCACTTCTAAATGCATGTA+TGG + chr3.4:82365536-82365555 MS.gene33658:intron 35.0%
CCAAACATAGCAACAAAGTA+CGG + chr3.4:82365944-82365963 MS.gene33658:CDS 35.0%
GAACTTAATTTCCACAGCTA+GGG + chr3.4:82365341-82365360 MS.gene33658:intron 35.0%
GAATACATTACAAGGAAACC+AGG - chr3.4:82365466-82365485 None:intergenic 35.0%
TCCATTGTTTCCAACTTCAA+GGG + chr3.4:82364736-82364755 MS.gene33658:CDS 35.0%
TCTAGCCACAAACTATCTTA+AGG - chr3.4:82365294-82365313 None:intergenic 35.0%
TGAACTTAATTTCCACAGCT+AGG + chr3.4:82365340-82365359 MS.gene33658:intron 35.0%
TTGAGCATAAGAGAGGATTT+AGG + chr3.4:82365510-82365529 MS.gene33658:intron 35.0%
TTTGGATTGACTGTGAGTAT+GGG - chr3.4:82365659-82365678 None:intergenic 35.0%
! ACTCTGTGTCAATGTGATTT+TGG + chr3.4:82365586-82365605 MS.gene33658:intron 35.0%
! TTGGCTAGCTCATCAATAAT+AGG - chr3.4:82365875-82365894 None:intergenic 35.0%
AAACCACATCTCATCCAATG+TGG - chr3.4:82365215-82365234 None:intergenic 40.0%
AACCACATCTCATCCAATGT+GGG - chr3.4:82365214-82365233 None:intergenic 40.0%
CCGTACTTTGTTGCTATGTT+TGG - chr3.4:82365947-82365966 None:intergenic 40.0%
CTCCATTGTTTCCAACTTCA+AGG + chr3.4:82364735-82364754 MS.gene33658:CDS 40.0%
CTTGAAGTTGGAAACAATGG+AGG - chr3.4:82364737-82364756 None:intergenic 40.0%
CTTGGGAAGAATTCCTAAAC+AGG + chr3.4:82365366-82365385 MS.gene33658:intron 40.0%
GTAAGACGAAAAATGTCTGC+AGG - chr3.4:82365069-82365088 None:intergenic 40.0%
GTTGTAGACAGAGTAGACTT+TGG - chr3.4:82366040-82366059 None:intergenic 40.0%
GTTTCCAACTTCAAGGGAAA+AGG + chr3.4:82364742-82364761 MS.gene33658:CDS 40.0%
GTTTGGATTGACTGTGAGTA+TGG - chr3.4:82365660-82365679 None:intergenic 40.0%
TCAACACTATCATTCCCATC+TGG + chr3.4:82364812-82364831 MS.gene33658:CDS 40.0%
TCCCTTGAAGTTGGAAACAA+TGG - chr3.4:82364740-82364759 None:intergenic 40.0%
TTGAGGATTGAGCATAAGAG+AGG + chr3.4:82365503-82365522 MS.gene33658:intron 40.0%
TTGGATTGACTGTGAGTATG+GGG - chr3.4:82365658-82365677 None:intergenic 40.0%
! ACAACCTTTTCCCTTGAAGT+TGG - chr3.4:82364749-82364768 None:intergenic 40.0%
! ATGTATGGATTGACGGTAGT+AGG + chr3.4:82365551-82365570 MS.gene33658:intron 40.0%
AACAATGGAGGAAGACACTG+AGG - chr3.4:82364725-82364744 None:intergenic 45.0%
ACATGCTCAAACTTGGCAGT+TGG + chr3.4:82365746-82365765 MS.gene33658:intron 45.0%
ATCCCACATTGGATGAGATG+TGG + chr3.4:82365209-82365228 MS.gene33658:intron 45.0%
CCAAAAATCTACCAGCACAG+GGG - chr3.4:82365819-82365838 None:intergenic 45.0%
CCCAAAAATCTACCAGCACA+GGG - chr3.4:82365820-82365839 None:intergenic 45.0%
CTCATCCAATGTGGGATTCT+TGG - chr3.4:82365206-82365225 None:intergenic 45.0%
CTGTGAGTATGGGGGAATTA+TGG - chr3.4:82365649-82365668 None:intergenic 45.0%
GACTAAACATGCTTGCTTCC+TGG + chr3.4:82365445-82365464 MS.gene33658:intron 45.0%
GAGCTAGCCAAAGAATATGC+TGG + chr3.4:82365884-82365903 MS.gene33658:CDS 45.0%
GCTCGTGAAGCTGTAAATGA+AGG + chr3.4:82364869-82364888 MS.gene33658:CDS 45.0%
GGGTTTCACTTGCAAGAACA+AGG - chr3.4:82365799-82365818 None:intergenic 45.0%
GTCTACTCTGTCTACAACTG+TGG + chr3.4:82366042-82366061 MS.gene33658:CDS 45.0%
TGGATTGACGGTAGTAGGTT+AGG + chr3.4:82365556-82365575 MS.gene33658:intron 45.0%
TGGATTGACTGTGAGTATGG+GGG - chr3.4:82365657-82365676 None:intergenic 45.0%
TTCTGTAGGCAACACCAGAT+GGG - chr3.4:82364829-82364848 None:intergenic 45.0%
TTTCTGTAGGCAACACCAGA+TGG - chr3.4:82364830-82364849 None:intergenic 45.0%
!! CCCTGTGCTGGTAGATTTTT+GGG + chr3.4:82365817-82365836 MS.gene33658:CDS 45.0%
!! TGGTAGATTTTTGGGCACCA+TGG + chr3.4:82365825-82365844 MS.gene33658:CDS 45.0%
ATAGGCGCTATCATCCTGCA+TGG - chr3.4:82365857-82365876 None:intergenic 50.0%
ATTTCCACAGCTAGGGGACT+TGG + chr3.4:82365348-82365367 MS.gene33658:intron 50.0%
CATCTCAAGCGTGACTTGAG+TGG - chr3.4:82365167-82365186 None:intergenic 50.0%
GCCCAAAAATCTACCAGCAC+AGG - chr3.4:82365821-82365840 None:intergenic 50.0%
GTGTTCTCAGCTCAAAGAGC+AGG + chr3.4:82364767-82364786 MS.gene33658:CDS 50.0%
TTTCCACAGCTAGGGGACTT+GGG + chr3.4:82365349-82365368 MS.gene33658:intron 50.0%
!! CCCCTGTGCTGGTAGATTTT+TGG + chr3.4:82365816-82365835 MS.gene33658:CDS 50.0%
!! GATTTTTGGGCACCATGGTG+TGG + chr3.4:82365830-82365849 MS.gene33658:CDS 50.0%
AAGACACTGAGGTCTGGCTG+TGG - chr3.4:82364714-82364733 None:intergenic 55.0%
ATCCTGCATGGACCACACCA+TGG - chr3.4:82365845-82365864 None:intergenic 55.0%
GTGCGCCAAGAATCCCACAT+TGG + chr3.4:82365198-82365217 MS.gene33658:intron 55.0%
TGCAAGTGAAACCCCTGTGC+TGG + chr3.4:82365805-82365824 MS.gene33658:CDS 55.0%
TGGAGGAAGACACTGAGGTC+TGG - chr3.4:82364720-82364739 None:intergenic 55.0%
! GAGCAGTGACTGACTCAAGC+TGG + chr3.4:82365768-82365787 MS.gene33658:CDS 55.0%
CACCATGGTGTGGTCCATGC+AGG + chr3.4:82365840-82365859 MS.gene33658:CDS 60.0%
CTTCCCAAGTCCCCTAGCTG+TGG - chr3.4:82365355-82365374 None:intergenic 60.0%
GTGGGATTCTTGGCGCACAC+AGG - chr3.4:82365196-82365215 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 82364683 82366084 82364683 ID=MS.gene33658
chr3.4 mRNA 82364683 82366084 82364683 ID=MS.gene33658.t1;Parent=MS.gene33658
chr3.4 exon 82364683 82364890 82364683 ID=MS.gene33658.t1.exon1;Parent=MS.gene33658.t1
chr3.4 CDS 82364683 82364890 82364683 ID=cds.MS.gene33658.t1;Parent=MS.gene33658.t1
chr3.4 exon 82365765 82366084 82365765 ID=MS.gene33658.t1.exon2;Parent=MS.gene33658.t1
chr3.4 CDS 82365765 82366084 82365765 ID=cds.MS.gene33658.t1;Parent=MS.gene33658.t1
Gene Sequence

>MS.gene33658

ATGGCTTTGGAGAATTGTTTAAGAGTATCCACAGCCAGACCTCAGTGTCTTCCTCCATTGTTTCCAACTTCAAGGGAAAAGGTTGTGTTCTCAGCTCAAAGAGCAGGATTCAAGAAATCTGTGCTGAACTCAACACTATCATTCCCATCTGGTGTTGCCTACAGAAAATCTCGTTTTATTTGCAATGCTCGTGAAGCTGTAAATGAAGGTATACTTTCATGTACCTTGTGAACTGTTTTTCTTGAAAAGTTGTGAAGAGATAGATAGTATAGTATCAAAAACTGCATCTGAATATTTTGTTATAAGTCTTTTACACTAGATATGACCTATATTTTGTTATGAGTCTTTTACACTAGATATGACCTGAAAATGAGTGTTTATAACCTGCAGACATTTTTCGTCTTACAAGTTGGTTTTATAATTGAGTTAGATCAATTTCAAATGTTAACATGATATCAGAGCTTATTTAAGATATTTTCGGCCACTCAAGTCACGCTTGAGATGTTCACTCCTGTGTGCGCCAAGAATCCCACATTGGATGAGATGTGGTTTGAAAATGAGTTTATAAATGAAAGACATTTGTAAGATTGAGTTAGCTTCAACTGAAACCTTAAGATAGTTTGTGGCTAGAGATACACCTTATCTTCACATTATAATTGAACTTAATTTCCACAGCTAGGGGACTTGGGAAGAATTCCTAAACAGGTTAAAACTTAATGTTCAGATAGCAGATAGAATATTCAATACCATCTTGTAAGTGATGACTAAACATGCTTGCTTCCTGGTTTCCTTGTAATGTATTCTGAATTTTATTTTCTCATTGAGGATTGAGCATAAGAGAGGATTTAGGTAGAGCACTTCTAAATGCATGTATGGATTGACGGTAGTAGGTTAGGCAGATTAACTCTGTGTCAATGTGATTTTGGCAAAACACTTTCGAGCTTCAATACAATCACAATATCACCATAATTCCCCCATACTCACAGTCAATCCAAACATGCAAAAGTTAATTTGCCTTAGGTTTATTCACTATAGTGTCTTGTCTAATGAAGTAATAACACATACATGCTCAAACTTGGCAGTTGGAGCAGTGACTGACTCAAGCTGGAATGACCTTGTTCTTGCAAGTGAAACCCCTGTGCTGGTAGATTTTTGGGCACCATGGTGTGGTCCATGCAGGATGATAGCGCCTATTATTGATGAGCTAGCCAAAGAATATGCTGGAAAAATTTCGTGCTACAAGCTCAACACAGATGAGAATCCAAACATAGCAACAAAGTACGGCATCAGAAGCATACCAACTGTTCTATTTTTCAAGAATGGAGAGAAAAAAGAAAGTGTAATTGGTGCAGTTCCAAAGTCTACTCTGTCTACAACTGTGGAAAAATATATTGATGCATGA

Protein sequence

>MS.gene33658.t1

MALENCLRVSTARPQCLPPLFPTSREKVVFSAQRAGFKKSVLNSTLSFPSGVAYRKSRFICNAREAVNEVGAVTDSSWNDLVLASETPVLVDFWAPWCGPCRMIAPIIDELAKEYAGKISCYKLNTDENPNIATKYGIRSIPTVLFFKNGEKKESVIGAVPKSTLSTTVEKYIDA