AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08513


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAATTATGCATACCTTTC+TGG 0.079710 8.1:-76179168 None:intergenic
CCTGGAGTTCTTGAGTTAAT+AGG 0.246781 8.1:+76178193 MS.gene08513:CDS
GATAGACTTCCTTTGATTCC+TGG 0.345188 8.1:+76178175 MS.gene08513:CDS
GTTCTTGAGTTAATAGGAAT+TGG 0.346748 8.1:+76178199 MS.gene08513:CDS
AAAGATTGCTCGTGATGTTA+TGG 0.420998 8.1:+76177777 MS.gene08513:CDS
TAGCTAGTGTATTTGCACTA+TGG 0.434144 8.1:+76178023 MS.gene08513:CDS
GATGTTATGGTAATGGCAAC+AGG 0.459632 8.1:+76177790 MS.gene08513:CDS
TTTGCACTATGGAGCTCAGC+TGG 0.465802 8.1:+76178034 MS.gene08513:CDS
TGAAGATTCTTAACAAACTC+AGG 0.486308 8.1:-76177847 None:intergenic
TCAGTTGAGTCAACTTCTGT+TGG 0.503744 8.1:-76177820 None:intergenic
TAAATATGCAGTGGGTTCAT+TGG 0.505381 8.1:+76177997 MS.gene08513:CDS
CCTATTAACTCAAGAACTCC+AGG 0.507872 8.1:-76178193 None:intergenic
TTAATAGGAATTGGTTACAC+TGG 0.524822 8.1:+76178208 MS.gene08513:CDS
TAATAGGAATTGGTTACACT+GGG 0.540472 8.1:+76178209 MS.gene08513:CDS
TCAAGAACTCCAGGAATCAA+AGG 0.544788 8.1:-76178184 None:intergenic
TGCTCGTGATGTTATGGTAA+TGG 0.557462 8.1:+76177783 MS.gene08513:CDS
TTTGATGATAAATATGCAGT+GGG 0.562064 8.1:+76177989 MS.gene08513:CDS
ATTTGATGATAAATATGCAG+TGG 0.570116 8.1:+76177988 MS.gene08513:CDS
AATAGGAATTGGTTACACTG+GGG 0.760636 8.1:+76178210 MS.gene08513:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAATAACATAATTCT+AGG - chr8.1:76177947-76177966 None:intergenic 10.0%
!! ATAAAAATGTATTACAATAT+CGG - chr8.1:76178683-76178702 None:intergenic 10.0%
!! TATATGTATATATATATATG+AGG + chr8.1:76179049-76179068 MS.gene08513:intron 10.0%
!! TATTTCAAAAAAAAAATACA+AGG - chr8.1:76178308-76178327 None:intergenic 10.0%
!!! AATTATGTTATTTATTTTGT+TGG + chr8.1:76177949-76177968 MS.gene08513:intron 10.0%
! GGGGGGGGGGGGGGGGGGGG+GGG - chr8.1:76178569-76178588 None:intergenic 100.0%
!! TCAATAATAAACATTAAGAA+GGG - chr8.1:76177924-76177943 None:intergenic 15.0%
!!! TATTTTGTCATAAAATCAAA+TGG - chr8.1:76178860-76178879 None:intergenic 15.0%
!! AAATATAGAAGATGAATACT+AGG - chr8.1:76179108-76179127 None:intergenic 20.0%
!! CTTAGATTTAAATATATGAC+TGG + chr8.1:76178250-76178269 MS.gene08513:intron 20.0%
!! GTCAATAATAAACATTAAGA+AGG - chr8.1:76177925-76177944 None:intergenic 20.0%
!! TCTAATCTTATCAATTTGTA+AGG - chr8.1:76178723-76178742 None:intergenic 20.0%
!! TTACAAATTGATAAGATTAG+AGG + chr8.1:76178722-76178741 MS.gene08513:intron 20.0%
!!! TTTTTTTCCAGTAATTTCTA+GGG + chr8.1:76178800-76178819 MS.gene08513:intron 20.0%
! TAGGTTGTTAGAAATTGTAA+TGG + chr8.1:76177887-76177906 MS.gene08513:intron 25.0%
!! ATTTGATGATAAATATGCAG+TGG + chr8.1:76177988-76178007 MS.gene08513:CDS 25.0%
!! TATGAGGTATTATTCATCTT+TGG + chr8.1:76179065-76179084 MS.gene08513:intron 25.0%
!! TTTGATGATAAATATGCAGT+GGG + chr8.1:76177989-76178008 MS.gene08513:CDS 25.0%
!!! AATATCGCGTATTTGATTTT+TGG + chr8.1:76178281-76178300 MS.gene08513:intron 25.0%
!!! GTTTTTTTCCAGTAATTTCT+AGG + chr8.1:76178799-76178818 MS.gene08513:intron 25.0%
!!! TTGTTTTTTAAAATGATCCC+CGG + chr8.1:76178345-76178364 MS.gene08513:intron 25.0%
AAGAAAACCCTAGAAATTAC+TGG - chr8.1:76178810-76178829 None:intergenic 30.0%
AAGCACAATAGTATTTGACA+TGG - chr8.1:76178595-76178614 None:intergenic 30.0%
AATATCGGACTCTATCTATA+AGG - chr8.1:76178668-76178687 None:intergenic 30.0%
AGCACAATAGTATTTGACAT+GGG - chr8.1:76178594-76178613 None:intergenic 30.0%
ATCAAATGGCTTTCTTAAGA+TGG - chr8.1:76178846-76178865 None:intergenic 30.0%
ATGTCAAATACTATTGTGCT+TGG + chr8.1:76178594-76178613 MS.gene08513:intron 30.0%
CAATTACTTTCACTTGTAAC+AGG + chr8.1:76178147-76178166 MS.gene08513:intron 30.0%
GCATCAAGTGAAATAATGTT+GGG - chr8.1:76178480-76178499 None:intergenic 30.0%
GCTTTCTTAAGATGGTATTT+AGG - chr8.1:76178838-76178857 None:intergenic 30.0%
GTTCTTGAGTTAATAGGAAT+TGG + chr8.1:76178199-76178218 MS.gene08513:CDS 30.0%
TAATAGGAATTGGTTACACT+GGG + chr8.1:76178209-76178228 MS.gene08513:CDS 30.0%
TGAAGATTCTTAACAAACTC+AGG - chr8.1:76177850-76177869 None:intergenic 30.0%
TGCATCAAGTGAAATAATGT+TGG - chr8.1:76178481-76178500 None:intergenic 30.0%
TTAATAGGAATTGGTTACAC+TGG + chr8.1:76178208-76178227 MS.gene08513:CDS 30.0%
! ACTTGATGCATTGATATACA+TGG + chr8.1:76178491-76178510 MS.gene08513:intron 30.0%
! TATTTTGTTGGCATCATAGT+GGG + chr8.1:76177961-76177980 MS.gene08513:intron 30.0%
!! TTATTTTGTTGGCATCATAG+TGG + chr8.1:76177960-76177979 MS.gene08513:intron 30.0%
!!! TGTTTTTTAAAATGATCCCC+GGG + chr8.1:76178346-76178365 MS.gene08513:intron 30.0%
AAAGATTGCTCGTGATGTTA+TGG + chr8.1:76177777-76177796 MS.gene08513:CDS 35.0%
AATAGGAATTGGTTACACTG+GGG + chr8.1:76178210-76178229 MS.gene08513:CDS 35.0%
ACAATAGTATTTGACATGGG+GGG - chr8.1:76178591-76178610 None:intergenic 35.0%
ATCAAATACGCGATATTTGC+AGG - chr8.1:76178278-76178297 None:intergenic 35.0%
ATTGACATATAGATAGGGGA+TGG + chr8.1:76178620-76178639 MS.gene08513:intron 35.0%
CAAGAAGCTGTATGATACAT+AGG + chr8.1:76177868-76177887 MS.gene08513:intron 35.0%
CACAATAGTATTTGACATGG+GGG - chr8.1:76178592-76178611 None:intergenic 35.0%
CCTGCATTGAATAGTGTATA+AGG - chr8.1:76178529-76178548 None:intergenic 35.0%
CCTTATACACTATTCAATGC+AGG + chr8.1:76178526-76178545 MS.gene08513:intron 35.0%
GCACAATAGTATTTGACATG+GGG - chr8.1:76178593-76178612 None:intergenic 35.0%
TAGCTAGTGTATTTGCACTA+TGG + chr8.1:76178023-76178042 MS.gene08513:CDS 35.0%
TCAAATACGCGATATTTGCA+GGG - chr8.1:76178277-76178296 None:intergenic 35.0%
TTCTATATTTCACCTTGCAG+TGG + chr8.1:76179118-76179137 MS.gene08513:intron 35.0%
! GCATTGATATACATGGTTGA+TGG + chr8.1:76178498-76178517 MS.gene08513:intron 35.0%
! GGTGCATTGACATATAGATA+GGG + chr8.1:76178615-76178634 MS.gene08513:intron 35.0%
! GTGCATTGACATATAGATAG+GGG + chr8.1:76178616-76178635 MS.gene08513:intron 35.0%
! TAAATATGCAGTGGGTTCAT+TGG + chr8.1:76177997-76178016 MS.gene08513:CDS 35.0%
! TGGTGCATTGACATATAGAT+AGG + chr8.1:76178614-76178633 MS.gene08513:intron 35.0%
!! GATGGCCTTATAGAAGATAT+GGG + chr8.1:76178638-76178657 MS.gene08513:intron 35.0%
AATAGTATTTGACATGGGGG+GGG - chr8.1:76178589-76178608 None:intergenic 40.0%
ACACTATTCAATGCAGGACT+TGG + chr8.1:76178532-76178551 MS.gene08513:intron 40.0%
CAATAGTATTTGACATGGGG+GGG - chr8.1:76178590-76178609 None:intergenic 40.0%
CCTATTAACTCAAGAACTCC+AGG - chr8.1:76178196-76178215 None:intergenic 40.0%
CCTGGAGTTCTTGAGTTAAT+AGG + chr8.1:76178193-76178212 MS.gene08513:CDS 40.0%
GATAGACTTCCTTTGATTCC+TGG + chr8.1:76178175-76178194 MS.gene08513:CDS 40.0%
GCCATCCCATATCTTCTATA+AGG - chr8.1:76178646-76178665 None:intergenic 40.0%
GCCTTATAGAAGATATGGGA+TGG + chr8.1:76178642-76178661 MS.gene08513:intron 40.0%
GTCCATTTAAGCTGATAGGT+GGG + chr8.1:76178368-76178387 MS.gene08513:intron 40.0%
TCAAGAACTCCAGGAATCAA+AGG - chr8.1:76178187-76178206 None:intergenic 40.0%
TCAGTTGAGTCAACTTCTGT+TGG - chr8.1:76177823-76177842 None:intergenic 40.0%
TGCTCGTGATGTTATGGTAA+TGG + chr8.1:76177783-76177802 MS.gene08513:CDS 40.0%
!! GATATACATGGTTGATGGTC+AGG + chr8.1:76178503-76178522 MS.gene08513:intron 40.0%
!! GATGTTATGGTAATGGCAAC+AGG + chr8.1:76177790-76177809 MS.gene08513:CDS 40.0%
!! GGATGGCCTTATAGAAGATA+TGG + chr8.1:76178637-76178656 MS.gene08513:intron 40.0%
ATAGTATTTGACATGGGGGG+GGG - chr8.1:76178588-76178607 None:intergenic 45.0%
CCTATCAGCTTAAATGGACC+CGG - chr8.1:76178367-76178386 None:intergenic 45.0%
CTATCAGCTTAAATGGACCC+GGG - chr8.1:76178366-76178385 None:intergenic 45.0%
CTCCCACCTATCAGCTTAAA+TGG - chr8.1:76178373-76178392 None:intergenic 45.0%
CTTGTATGCAAACCACTGCA+AGG - chr8.1:76179133-76179152 None:intergenic 45.0%
GGTCCATTTAAGCTGATAGG+TGG + chr8.1:76178367-76178386 MS.gene08513:intron 45.0%
TATCAGCTTAAATGGACCCG+GGG - chr8.1:76178365-76178384 None:intergenic 45.0%
!!! GAGCTCAGCTGGTTTTATCT+CGG + chr8.1:76178045-76178064 MS.gene08513:CDS 45.0%
CCGGGTCCATTTAAGCTGAT+AGG + chr8.1:76178364-76178383 MS.gene08513:intron 50.0%
TAGTATTTGACATGGGGGGG+GGG - chr8.1:76178587-76178606 None:intergenic 50.0%
TTTGCACTATGGAGCTCAGC+TGG + chr8.1:76178034-76178053 MS.gene08513:CDS 50.0%
AGTATTTGACATGGGGGGGG+GGG - chr8.1:76178586-76178605 None:intergenic 55.0%
GTATTTGACATGGGGGGGGG+GGG - chr8.1:76178585-76178604 None:intergenic 60.0%
TATTTGACATGGGGGGGGGG+GGG - chr8.1:76178584-76178603 None:intergenic 60.0%
ATTTGACATGGGGGGGGGGG+GGG - chr8.1:76178583-76178602 None:intergenic 65.0%
TTTGACATGGGGGGGGGGGG+GGG - chr8.1:76178582-76178601 None:intergenic 70.0%
TTGACATGGGGGGGGGGGGG+GGG - chr8.1:76178581-76178600 None:intergenic 75.0%
! TGACATGGGGGGGGGGGGGG+GGG - chr8.1:76178580-76178599 None:intergenic 80.0%
! ACATGGGGGGGGGGGGGGGG+GGG - chr8.1:76178578-76178597 None:intergenic 85.0%
! GACATGGGGGGGGGGGGGGG+GGG - chr8.1:76178579-76178598 None:intergenic 85.0%
! ATGGGGGGGGGGGGGGGGGG+GGG - chr8.1:76178576-76178595 None:intergenic 90.0%
! CATGGGGGGGGGGGGGGGGG+GGG - chr8.1:76178577-76178596 None:intergenic 90.0%
! TGGGGGGGGGGGGGGGGGGG+GGG - chr8.1:76178575-76178594 None:intergenic 95.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 76177773 76179175 76177773 ID=MS.gene08513
chr8.1 mRNA 76177773 76179175 76177773 ID=MS.gene08513.t1;Parent=MS.gene08513
chr8.1 exon 76177773 76177876 76177773 ID=MS.gene08513.t1.exon1;Parent=MS.gene08513.t1
chr8.1 CDS 76177773 76177876 76177773 ID=cds.MS.gene08513.t1;Parent=MS.gene08513.t1
chr8.1 exon 76177980 76178066 76177980 ID=MS.gene08513.t1.exon2;Parent=MS.gene08513.t1
chr8.1 CDS 76177980 76178066 76177980 ID=cds.MS.gene08513.t1;Parent=MS.gene08513.t1
chr8.1 exon 76178169 76178231 76178169 ID=MS.gene08513.t1.exon3;Parent=MS.gene08513.t1
chr8.1 CDS 76178169 76178231 76178169 ID=cds.MS.gene08513.t1;Parent=MS.gene08513.t1
chr8.1 exon 76179138 76179175 76179138 ID=MS.gene08513.t1.exon4;Parent=MS.gene08513.t1
chr8.1 CDS 76179138 76179175 76179138 ID=cds.MS.gene08513.t1;Parent=MS.gene08513.t1
Gene Sequence

>MS.gene08513

ATCGAAAGATTGCTCGTGATGTTATGGTAATGGCAACAGGAGATGCACCAACAGAAGTTGACTCAACTGAGCTACCTGAGTTTGTTAAGAATCTTCAAGAAGCTGTATGATACATAGGTTGTTAGAAATTGTAATGGAGTTTCTTTTTCCCTTCTTAATGTTTATTATTGACCTAGAATTATGTTATTTATTTTGTTGGCATCATAGTGGGACAAATTTGATGATAAATATGCAGTGGGTTCATTGGTTGTAGCTAGTGTATTTGCACTATGGAGCTCAGCTGGTTTTATCTCGGTATTAAGTTATTTAAATGCTAATTTTATTTGCATTATTTGATTATCAATTTATTAGATACAGATGATAAATGTCATAAGCAATTACTTTCACTTGTAACAGGCAATTGATAGACTTCCTTTGATTCCTGGAGTTCTTGAGTTAATAGGAATTGGTTACACTGGGGTAAGTCAAATAAATACACTTAGATTTAAATATATGACTGGTCCCTGCAAATATCGCGTATTTGATTTTTGGTCCTTGTATTTTTTTTTTGAAATACATCTTTGCAAGAAAAATTGTTTTTTAAAATGATCCCCGGGTCCATTTAAGCTGATAGGTGGGAGTCTGATGCTTACGTTACACATGCTAAAAAAAATAAAATCTGAAACTTAATTTAAAAAAAAAATATAAATAGATTTTTTCATATGCCCAACATTATTTCACTTGATGCATTGATATACATGGTTGATGGTCAGGCCTTATACACTATTCAATGCAGGACTTGGATTTTTGTTTTACACCCCCCCCCCCCCCCCCCCCCCCCCATGTCAAATACTATTGTGCTTGGTGCATTGACATATAGATAGGGGATGGCCTTATAGAAGATATGGGATGGCCTTATAGATAGAGTCCGATATTGTAATACATTTTTATATTTAGACATAACTCATCCTTACAAATTGATAAGATTAGAGGTGTCACTCTTTATAAACTCATCTCAAGCATTATATTTATTCAATGCGACACTTAGTTTTTTTCCAGTAATTTCTAGGGTTTTCTTTTAATCCTAAATACCATCTTAAGAAAGCCATTTGATTTTATGACAAAATACATATATACTAGCAAAAACAATATATATATATATATATATGTATATATATCTATATATATATATGTATATATATATCTATATATGTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGTGTATATATATATATATATGTATATATATATATGAGGTATTATTCATCTTTGGATTCAACTATAAGAAAACCTAGTATTCATCTTCTATATTTCACCTTGCAGTGGTTTGCATACAAGAACGTAGTTTTCAAGCCAGAAAG

Protein sequence

>MS.gene08513.t1

IERLLVMLWUWQQEMHQQKLTQLSYLSLLRIFKKLGTNLMINMQWVHWLULVYLHYGAQLVLSRQLIDFLUFLEFLSUUELVTLGGLHTRTUFSSQK