AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99819


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99819.t1 MTR_2g082930 97.209 215 6 0 1 215 1 215 3.85e-156 431
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99819.t1 AT1G51100 69.780 182 47 4 37 215 35 211 1.20e-81 242

Find 38 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCAACCTATTTCAAAATT+TGG 0.094353 2.3:+14671361 None:intergenic
TTCTCTATAGGAAATGTTTC+TGG 0.167892 2.3:+14671448 None:intergenic
TCAGAAGATGTGGAATTTAA+TGG 0.265594 2.3:+14671517 None:intergenic
CTGTTCATTGTAGTGTTTGT+AGG 0.309624 2.3:+14671472 None:intergenic
GAGGTTGATGGAAATGGATT+TGG 0.355148 2.3:-14671122 MS.gene99819:CDS
GAACTTATGAAGAAGGAATA+TGG 0.383069 2.3:-14671268 MS.gene99819:CDS
ATTGTAGTGTTTGTAGGGTC+TGG 0.399199 2.3:+14671478 None:intergenic
TGTTCATTGTAGTGTTTGTA+GGG 0.442377 2.3:+14671473 None:intergenic
GTTGGAGGAACTTATGAAGA+AGG 0.476939 2.3:-14671275 MS.gene99819:CDS
TGGCTCGGCATTTGCAAGGT+CGG 0.477066 2.3:+14671384 None:intergenic
CAACCTATTTCAAAATTTGG+TGG 0.478009 2.3:+14671364 None:intergenic
AGGAAAAGTTGAGGAGAGAA+TGG 0.490855 2.3:-14671167 MS.gene99819:CDS
ATGAAGAGTTAAAATTCAAA+AGG 0.492536 2.3:+14671006 None:intergenic
GAGAGATGAAGGAAAAGTTG+AGG 0.497620 2.3:-14671176 MS.gene99819:CDS
ACAGAACTGTTGGCAAAAGT+TGG 0.501386 2.3:-14671241 MS.gene99819:CDS
TATTTCAAAATTTGGTGGCT+CGG 0.505623 2.3:+14671369 None:intergenic
TGAAAGGTGTGAAGATGTGT+TGG 0.506225 2.3:+14671588 None:intergenic
TATGGTGAAAGCTTCTGTTA+AGG 0.539994 2.3:-14671098 MS.gene99819:CDS
AGGAAGATACTTTGAATGAG+AGG 0.541636 2.3:-14671078 MS.gene99819:CDS
TTGTGTAGATGATGAGGTGT+AGG 0.543834 2.3:+14671622 None:intergenic
ATATGGTTCAACAGAACTGT+TGG 0.553725 2.3:-14671251 MS.gene99819:CDS
GATGGAAATGGATTTGGCTA+TGG 0.555092 2.3:-14671116 MS.gene99819:CDS
AAATTCTTGCTCAGAAGATG+TGG 0.558444 2.3:+14671507 None:intergenic
CAGAGATAATCAGACAAAGA+AGG 0.559049 2.3:-14671414 MS.gene99819:CDS
AGTGTTTGTAGGGTCTGGTG+TGG 0.564863 2.3:+14671483 None:intergenic
TCAGCTACCACATACCCTAC+TGG 0.567101 2.3:+14671310 None:intergenic
TGAGAAGCCAGTAGGGTATG+TGG 0.573091 2.3:-14671317 MS.gene99819:CDS
TTGGTGGCTCGGCATTTGCA+AGG 0.573166 2.3:+14671380 None:intergenic
ATCAAGCTAAGGCTAGGTGC+AGG 0.575411 2.3:-14671051 MS.gene99819:CDS
ATTAACCTTGAGAAGCCAGT+AGG 0.578990 2.3:-14671325 MS.gene99819:CDS
GTGTTTGTAGGGTCTGGTGT+GGG 0.588160 2.3:+14671484 None:intergenic
TGAGAGGCTTGATCAAGCTA+AGG 0.591500 2.3:-14671062 MS.gene99819:CDS
TGTGAGGAATGTTGAAGCCA+TGG 0.619146 2.3:+14671648 None:intergenic
GGCTTGATCAAGCTAAGGCT+AGG 0.619994 2.3:-14671057 MS.gene99819:CDS
TTAACCTTGAGAAGCCAGTA+GGG 0.629422 2.3:-14671324 MS.gene99819:CDS
CATACCCTACTGGCTTCTCA+AGG 0.644603 2.3:+14671320 None:intergenic
AAGAGATTGTGTAGATGATG+AGG 0.673918 2.3:+14671616 None:intergenic
GATGAGGTGTAGGATATGTG+AGG 0.690908 2.3:+14671632 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATGAAAAAGTGCTTTT+TGG + chr2.3:14671121-14671140 None:intergenic 20.0%
! TTCCAACCTATTTCAAAATT+TGG + chr2.3:14671309-14671328 None:intergenic 25.0%
!!! ATTTTGAAATAGGTTGGAAA+AGG - chr2.3:14671310-14671329 MS.gene99819:CDS 25.0%
CAACCTATTTCAAAATTTGG+TGG + chr2.3:14671306-14671325 None:intergenic 30.0%
GAACTTATGAAGAAGGAATA+TGG - chr2.3:14671399-14671418 MS.gene99819:CDS 30.0%
TCAGAAGATGTGGAATTTAA+TGG + chr2.3:14671153-14671172 None:intergenic 30.0%
TGTTCATTGTAGTGTTTGTA+GGG + chr2.3:14671197-14671216 None:intergenic 30.0%
TTCTCTATAGGAAATGTTTC+TGG + chr2.3:14671222-14671241 None:intergenic 30.0%
! GATCTTCTTCTTTTCTCTAT+AGG + chr2.3:14671234-14671253 None:intergenic 30.0%
! TATTTCAAAATTTGGTGGCT+CGG + chr2.3:14671301-14671320 None:intergenic 30.0%
!!! CACCAAATTTTGAAATAGGT+TGG - chr2.3:14671304-14671323 MS.gene99819:CDS 30.0%
!!! TTGAACTCTTTAGAGTTTTG+AGG - chr2.3:14671526-14671545 MS.gene99819:CDS 30.0%
!!! TTTTGTTGTTGTGAGTTGAA+AGG + chr2.3:14671098-14671117 None:intergenic 30.0%
AAATTCTTGCTCAGAAGATG+TGG + chr2.3:14671163-14671182 None:intergenic 35.0%
AAGAGATTGTGTAGATGATG+AGG + chr2.3:14671054-14671073 None:intergenic 35.0%
AGGAAGATACTTTGAATGAG+AGG - chr2.3:14671589-14671608 MS.gene99819:CDS 35.0%
ATATGGTTCAACAGAACTGT+TGG - chr2.3:14671416-14671435 MS.gene99819:CDS 35.0%
CAGAGATAATCAGACAAAGA+AGG - chr2.3:14671253-14671272 MS.gene99819:CDS 35.0%
CTGTTCATTGTAGTGTTTGT+AGG + chr2.3:14671198-14671217 None:intergenic 35.0%
TATGGTGAAAGCTTCTGTTA+AGG - chr2.3:14671569-14671588 MS.gene99819:CDS 35.0%
!! GCAGAAATTTTGAGAGATGA+AGG - chr2.3:14671480-14671499 MS.gene99819:CDS 35.0%
!!! AGCTGATTTTTTGGAGAAGT+TGG - chr2.3:14671374-14671393 MS.gene99819:CDS 35.0%
!!! AGTTTTGAGGTTGATGGAAA+TGG - chr2.3:14671539-14671558 MS.gene99819:CDS 35.0%
!!! CTTTAGAGTTTTGAGGTTGA+TGG - chr2.3:14671533-14671552 MS.gene99819:CDS 35.0%
!!! GAGCCACCAAATTTTGAAAT+AGG - chr2.3:14671300-14671319 MS.gene99819:CDS 35.0%
!!! GTATGTGGTAGCTGATTTTT+TGG - chr2.3:14671365-14671384 MS.gene99819:CDS 35.0%
!!! TGATTTTTTGGAGAAGTTGG+AGG - chr2.3:14671377-14671396 MS.gene99819:CDS 35.0%
ACAGAACTGTTGGCAAAAGT+TGG - chr2.3:14671426-14671445 MS.gene99819:CDS 40.0%
AGGAAAAGTTGAGGAGAGAA+TGG - chr2.3:14671500-14671519 MS.gene99819:CDS 40.0%
ATTAACCTTGAGAAGCCAGT+AGG - chr2.3:14671342-14671361 MS.gene99819:CDS 40.0%
ATTGTAGTGTTTGTAGGGTC+TGG + chr2.3:14671192-14671211 None:intergenic 40.0%
GAGAGATGAAGGAAAAGTTG+AGG - chr2.3:14671491-14671510 MS.gene99819:CDS 40.0%
GTTGGAGGAACTTATGAAGA+AGG - chr2.3:14671392-14671411 MS.gene99819:CDS 40.0%
TTAACCTTGAGAAGCCAGTA+GGG - chr2.3:14671343-14671362 MS.gene99819:CDS 40.0%
TTGTGTAGATGATGAGGTGT+AGG + chr2.3:14671048-14671067 None:intergenic 40.0%
! GATGGAAATGGATTTGGCTA+TGG - chr2.3:14671551-14671570 MS.gene99819:CDS 40.0%
! TGAAAGGTGTGAAGATGTGT+TGG + chr2.3:14671082-14671101 None:intergenic 40.0%
!! GAGGTTGATGGAAATGGATT+TGG - chr2.3:14671545-14671564 MS.gene99819:CDS 40.0%
GATGAGGTGTAGGATATGTG+AGG + chr2.3:14671038-14671057 None:intergenic 45.0%
TGAGAGGCTTGATCAAGCTA+AGG - chr2.3:14671605-14671624 MS.gene99819:CDS 45.0%
!! GCATTTGCAAGGTCGGTTTT+CGG + chr2.3:14671279-14671298 None:intergenic 45.0%
AGTGTTTGTAGGGTCTGGTG+TGG + chr2.3:14671187-14671206 None:intergenic 50.0%
ATCAAGCTAAGGCTAGGTGC+AGG - chr2.3:14671616-14671635 MS.gene99819:CDS 50.0%
GGCTTGATCAAGCTAAGGCT+AGG - chr2.3:14671610-14671629 MS.gene99819:CDS 50.0%
GTGTTTGTAGGGTCTGGTGT+GGG + chr2.3:14671186-14671205 None:intergenic 50.0%
TCAGCTACCACATACCCTAC+TGG + chr2.3:14671360-14671379 None:intergenic 50.0%
TGAGAAGCCAGTAGGGTATG+TGG - chr2.3:14671350-14671369 MS.gene99819:CDS 50.0%
!! CATACCCTACTGGCTTCTCA+AGG + chr2.3:14671350-14671369 None:intergenic 50.0%
! TGGCTCGGCATTTGCAAGGT+CGG + chr2.3:14671286-14671305 None:intergenic 55.0%
! TTGGTGGCTCGGCATTTGCA+AGG + chr2.3:14671290-14671309 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 14671021 14671668 14671021 ID=MS.gene99819
chr2.3 mRNA 14671021 14671668 14671021 ID=MS.gene99819.t1;Parent=MS.gene99819
chr2.3 exon 14671021 14671668 14671021 ID=MS.gene99819.t1.exon1;Parent=MS.gene99819.t1
chr2.3 CDS 14671021 14671668 14671021 ID=cds.MS.gene99819.t1;Parent=MS.gene99819.t1
Gene Sequence

>MS.gene99819

ATGGCTTCAACATTCCTCACATATCCTACACCTCATCATCTACACAATCTCTTTCAAACCAACACATCTTCACACCTTTCAACTCACAACAACAAAACCAAAAAGCACTTTTTCATAAAATGCACTACTCCATTAAATTCCACATCTTCTGAGCAAGAATTTCCCACACCAGACCCTACAAACACTACAATGAACAGTCCAGAAACATTTCCTATAGAGAAAAGAAGAAGATCAGAGATAATCAGACAAAGAAGGCCGAAAACCGACCTTGCAAATGCCGAGCCACCAAATTTTGAAATAGGTTGGAAAAGGACAAAAGAGATTAACCTTGAGAAGCCAGTAGGGTATGTGGTAGCTGATTTTTTGGAGAAGTTGGAGGAACTTATGAAGAAGGAATATGGTTCAACAGAACTGTTGGCAAAAGTTGGTGAAATTGTTGCTGAAAGAGCAAGAGAAGAAGCAGAAATTTTGAGAGATGAAGGAAAAGTTGAGGAGAGAATGGTTGTTGAACTCTTTAGAGTTTTGAGGTTGATGGAAATGGATTTGGCTATGGTGAAAGCTTCTGTTAAGGAAGATACTTTGAATGAGAGGCTTGATCAAGCTAAGGCTAGGTGCAGGCAAGCTATACTTGTTTGTTATTCCTTTTGA

Protein sequence

>MS.gene99819.t1

MASTFLTYPTPHHLHNLFQTNTSSHLSTHNNKTKKHFFIKCTTPLNSTSSEQEFPTPDPTNTTMNSPETFPIEKRRRSEIIRQRRPKTDLANAEPPNFEIGWKRTKEINLEKPVGYVVADFLEKLEELMKKEYGSTELLAKVGEIVAERAREEAEILRDEGKVEERMVVELFRVLRLMEMDLAMVKASVKEDTLNERLDQAKARCRQAILVCYSF