AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062227


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062227.t1 MTR_1g076840 96.241 133 3 1 3 135 2 132 5.41e-91 259
MS.gene062227.t1 MTR_6g060170 82.474 97 17 0 39 135 2 98 1.70e-57 174
MS.gene062227.t1 MTR_1g069370 60.440 91 36 0 45 135 9 99 4.59e-38 125
MS.gene062227.t1 MTR_7g099200 59.341 91 37 0 45 135 5 95 2.70e-37 123
MS.gene062227.t1 MTR_6g059920 53.763 93 43 0 40 132 33 125 1.54e-30 106
MS.gene062227.t1 MTR_5g005820 51.087 92 45 0 40 131 2 93 2.45e-26 95.1
MS.gene062227.t1 MTR_6g060000 86.047 43 6 0 76 118 19 61 3.66e-22 83.6
MS.gene062227.t1 MTR_6g060090 68.000 50 14 1 68 117 9 56 2.21e-18 73.6
MS.gene062227.t1 MTR_1g086000 32.500 80 54 0 37 116 39 118 1.41e-12 61.2
MS.gene062227.t1 MTR_6g060030 55.172 58 8 1 96 135 7 64 1.63e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062227.t1 AT4G10300 67.669 133 39 3 6 135 3 134 1.03e-59 181
MS.gene062227.t1 AT3G04300 56.044 91 40 0 45 135 1 91 1.65e-35 118
MS.gene062227.t1 AT4G28703 48.454 97 41 1 47 134 8 104 4.92e-29 102
MS.gene062227.t1 AT4G10280 48.454 97 47 1 42 135 43 139 1.55e-26 97.1
MS.gene062227.t1 AT4G10290 43.617 94 50 1 42 132 15 108 3.28e-20 80.1
MS.gene062227.t1 AT2G32180 30.380 79 55 0 41 119 40 118 9.89e-12 58.9
MS.gene062227.t1 AT2G32180 30.380 79 55 0 41 119 40 118 9.89e-12 58.9
MS.gene062227.t1 AT2G32650 30.380 79 55 0 41 119 40 118 1.09e-11 58.5
MS.gene062227.t1 AT2G32650 30.380 79 55 0 41 119 40 118 1.09e-11 58.5

Find 34 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGTACAACTCATCCCTTT+TGG 0.324093 1.1:-57625725 None:intergenic
TGAAATTTCTGCTGGTGATT+TGG 0.359064 1.1:+57625694 MS.gene062227:CDS
AACAGAGACATGCTATCTTT+TGG 0.368243 1.1:+57625628 MS.gene062227:CDS
CAAAAGGGATGAGTTGTACT+TGG 0.379437 1.1:+57625726 MS.gene062227:CDS
CTTGGTAGCAGTCAAATGTT+TGG 0.389927 1.1:-57625023 None:intergenic
ACAACTCTTCTCCTTGTTGA+AGG 0.406999 1.1:-57625067 None:intergenic
AGCTCACTCAACTTGGTGTT+AGG 0.443465 1.1:+57625149 MS.gene062227:CDS
AACACCAAGTTGAGTGAGCT+TGG 0.455326 1.1:-57625146 None:intergenic
AGTTGAGTGAGCTTGGACTC+AGG 0.458878 1.1:-57625139 None:intergenic
GGCAAAGTTACACTTACCTT+GGG 0.470688 1.1:-57625046 None:intergenic
CACTCCTAGTGGGGCTAATG+AGG 0.472036 1.1:+57625667 MS.gene062227:CDS
TATGTCCATGGAAAATTACT+TGG 0.480875 1.1:-57625599 None:intergenic
CTCAACTTGGTGTTAGGCAG+TGG 0.492268 1.1:+57625155 MS.gene062227:CDS
TGTACTTGGGATGTTTCTGT+TGG 0.499364 1.1:+57625740 MS.gene062227:CDS
AGGCAAAGTTACACTTACCT+TGG 0.501016 1.1:-57625047 None:intergenic
TTGGGATGTTTCTGTTGGTG+TGG 0.510879 1.1:+57625745 MS.gene062227:CDS
GAGTCCAAGCTCACTCAACT+TGG 0.526860 1.1:+57625142 MS.gene062227:CDS
AGTTACACTTACCTTGGGCT+TGG 0.544555 1.1:-57625041 None:intergenic
AGTCAAATGTTTGGTTTGTG+AGG 0.547845 1.1:-57625014 None:intergenic
GTCCATGGAAAATTACTTGG+AGG 0.553197 1.1:-57625596 None:intergenic
GCAGCTGTCACAGAGAAACT+TGG 0.559175 1.1:+57625094 MS.gene062227:CDS
AAGTGAAGGTCACTCCTAGT+GGG 0.564562 1.1:+57625657 MS.gene062227:CDS
TCCATGGAAAATTACTTGGA+GGG 0.566600 1.1:-57625595 None:intergenic
AAAGTGAAGGTCACTCCTAG+TGG 0.587646 1.1:+57625656 MS.gene062227:CDS
GAGGCAGTTGAAATTTCTGC+TGG 0.589901 1.1:+57625686 MS.gene062227:CDS
TGAGTGAGCTTGGACTCAGG+AGG 0.606187 1.1:-57625136 None:intergenic
AAAAGGGATGAGTTGTACTT+GGG 0.609307 1.1:+57625727 MS.gene062227:CDS
ATGGCATCATCATCATGTGT+AGG 0.613363 1.1:+57624980 MS.gene062227:CDS
GTGTAACTTTGCCTTCAACA+AGG 0.618419 1.1:+57625056 MS.gene062227:CDS
TCTGTTGCATCATATGTCCA+TGG 0.622988 1.1:-57625611 None:intergenic
GAGTGAGCTTGGACTCAGGA+GGG 0.632998 1.1:-57625135 None:intergenic
ACTGCCTCATTAGCCCCACT+AGG 0.662701 1.1:-57625671 None:intergenic
TTTGACTGCTACCAAGCCCA+AGG 0.672230 1.1:+57625030 MS.gene062227:CDS
AGTGAAGGTCACTCCTAGTG+GGG 0.682734 1.1:+57625658 MS.gene062227:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTTTATTTTAATGAAAA+GGG + chr1.1:57625287-57625306 MS.gene062227:intron 10.0%
!!! TGATTTTTATTTTAATGAAA+AGG + chr1.1:57625286-57625305 MS.gene062227:intron 10.0%
!! ACAACATAAAAAATCTTTCA+CGG + chr1.1:57625484-57625503 MS.gene062227:intron 20.0%
!! TACTGTTAAAGTCATATATA+TGG - chr1.1:57625412-57625431 None:intergenic 20.0%
!!! AAACGTTTTTTATGATTTGA+CGG + chr1.1:57625447-57625466 MS.gene062227:intron 20.0%
! AACAAGATAATGAAGAAATG+TGG - chr1.1:57625193-57625212 None:intergenic 25.0%
! AAGAAATGTGGTATTTACTT+GGG - chr1.1:57625181-57625200 None:intergenic 25.0%
! ATATGACTTTAACAGTAGTT+AGG + chr1.1:57625415-57625434 MS.gene062227:intron 25.0%
! TGGTGTAAATAAAAACAGAT+GGG + chr1.1:57625568-57625587 MS.gene062227:intron 25.0%
! TTAACGAGATTAATACACTA+TGG + chr1.1:57625365-57625384 MS.gene062227:intron 25.0%
! TTAGAATAATAGACATGATG+AGG - chr1.1:57625224-57625243 None:intergenic 25.0%
! TTGGTGTAAATAAAAACAGA+TGG + chr1.1:57625567-57625586 MS.gene062227:intron 25.0%
!!! GTTTCTTACATTTTTTCTCA+TGG + chr1.1:57625252-57625271 MS.gene062227:intron 25.0%
GAAGAAATGTGGTATTTACT+TGG - chr1.1:57625182-57625201 None:intergenic 30.0%
GGTGTAAATAAAAACAGATG+GGG + chr1.1:57625569-57625588 MS.gene062227:intron 30.0%
TATGTCCATGGAAAATTACT+TGG - chr1.1:57625602-57625621 None:intergenic 30.0%
!! ATTTGGTTGTTTTCCCAAAA+GGG + chr1.1:57625711-57625730 MS.gene062227:CDS 30.0%
!!! TCTTACATTTTTTCTCATGG+TGG + chr1.1:57625255-57625274 MS.gene062227:intron 30.0%
AAAAGGGATGAGTTGTACTT+GGG + chr1.1:57625727-57625746 MS.gene062227:CDS 35.0%
TCCATGGAAAATTACTTGGA+GGG - chr1.1:57625598-57625617 None:intergenic 35.0%
TGAAATTTCTGCTGGTGATT+TGG + chr1.1:57625694-57625713 MS.gene062227:CDS 35.0%
! AACAGAGACATGCTATCTTT+TGG + chr1.1:57625628-57625647 MS.gene062227:CDS 35.0%
! AAGTACAACTCATCCCTTTT+GGG - chr1.1:57625727-57625746 None:intergenic 35.0%
! TGACTGTGTACACACATTTT+TGG + chr1.1:57625548-57625567 MS.gene062227:intron 35.0%
!! AGTCAAATGTTTGGTTTGTG+AGG - chr1.1:57625017-57625036 None:intergenic 35.0%
!! GATTTGGTTGTTTTCCCAAA+AGG + chr1.1:57625710-57625729 MS.gene062227:CDS 35.0%
!! TCTTTTGGAAGGAAAAGTGA+AGG + chr1.1:57625643-57625662 MS.gene062227:CDS 35.0%
ACAACTCTTCTCCTTGTTGA+AGG - chr1.1:57625070-57625089 None:intergenic 40.0%
ACTAGAAGAGCAACTAGCAT+AGG - chr1.1:57625346-57625365 None:intergenic 40.0%
AGGCAAAGTTACACTTACCT+TGG - chr1.1:57625050-57625069 None:intergenic 40.0%
ATGGCATCATCATCATGTGT+AGG + chr1.1:57624980-57624999 MS.gene062227:CDS 40.0%
CAAAAGGGATGAGTTGTACT+TGG + chr1.1:57625726-57625745 MS.gene062227:CDS 40.0%
CTAGAAGAGCAACTAGCATA+GGG - chr1.1:57625345-57625364 None:intergenic 40.0%
GGCAAAGTTACACTTACCTT+GGG - chr1.1:57625049-57625068 None:intergenic 40.0%
GTCCATGGAAAATTACTTGG+AGG - chr1.1:57625599-57625618 None:intergenic 40.0%
GTGTAACTTTGCCTTCAACA+AGG + chr1.1:57625056-57625075 MS.gene062227:CDS 40.0%
TCTGTTGCATCATATGTCCA+TGG - chr1.1:57625614-57625633 None:intergenic 40.0%
! CAAGTACAACTCATCCCTTT+TGG - chr1.1:57625728-57625747 None:intergenic 40.0%
! CTTGGTAGCAGTCAAATGTT+TGG - chr1.1:57625026-57625045 None:intergenic 40.0%
! GAGACATGCTATCTTTTGGA+AGG + chr1.1:57625632-57625651 MS.gene062227:CDS 40.0%
! TGTACTTGGGATGTTTCTGT+TGG + chr1.1:57625740-57625759 MS.gene062227:CDS 40.0%
AAAGTGAAGGTCACTCCTAG+TGG + chr1.1:57625656-57625675 MS.gene062227:CDS 45.0%
AAGTGAAGGTCACTCCTAGT+GGG + chr1.1:57625657-57625676 MS.gene062227:CDS 45.0%
AGTTACACTTACCTTGGGCT+TGG - chr1.1:57625044-57625063 None:intergenic 45.0%
GAGGCAGTTGAAATTTCTGC+TGG + chr1.1:57625686-57625705 MS.gene062227:CDS 45.0%
! AACACCAAGTTGAGTGAGCT+TGG - chr1.1:57625149-57625168 None:intergenic 45.0%
! GCCCTCCAAGTAATTTTCCA+TGG + chr1.1:57625594-57625613 MS.gene062227:CDS 45.0%
! TTGGGATGTTTCTGTTGGTG+TGG + chr1.1:57625745-57625764 MS.gene062227:CDS 45.0%
!! AGCTCACTCAACTTGGTGTT+AGG + chr1.1:57625149-57625168 MS.gene062227:CDS 45.0%
AGTGAAGGTCACTCCTAGTG+GGG + chr1.1:57625658-57625677 MS.gene062227:CDS 50.0%
AGTTGAGTGAGCTTGGACTC+AGG - chr1.1:57625142-57625161 None:intergenic 50.0%
GAGTCCAAGCTCACTCAACT+TGG + chr1.1:57625142-57625161 MS.gene062227:CDS 50.0%
GCAGCTGTCACAGAGAAACT+TGG + chr1.1:57625094-57625113 MS.gene062227:CDS 50.0%
TTTGACTGCTACCAAGCCCA+AGG + chr1.1:57625030-57625049 MS.gene062227:CDS 50.0%
!! CTCAACTTGGTGTTAGGCAG+TGG + chr1.1:57625155-57625174 MS.gene062227:CDS 50.0%
ACTGCCTCATTAGCCCCACT+AGG - chr1.1:57625674-57625693 None:intergenic 55.0%
CACTCCTAGTGGGGCTAATG+AGG + chr1.1:57625667-57625686 MS.gene062227:CDS 55.0%
GAGTGAGCTTGGACTCAGGA+GGG - chr1.1:57625138-57625157 None:intergenic 55.0%
TGAGTGAGCTTGGACTCAGG+AGG - chr1.1:57625139-57625158 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 57624980 57625790 57624980 ID=MS.gene062227
chr1.1 mRNA 57624980 57625790 57624980 ID=MS.gene062227.t1;Parent=MS.gene062227
chr1.1 exon 57624980 57625182 57624980 ID=MS.gene062227.t1.exon1;Parent=MS.gene062227.t1
chr1.1 CDS 57624980 57625182 57624980 ID=cds.MS.gene062227.t1;Parent=MS.gene062227.t1
chr1.1 exon 57625586 57625790 57625586 ID=MS.gene062227.t1.exon2;Parent=MS.gene062227.t1
chr1.1 CDS 57625586 57625790 57625586 ID=cds.MS.gene062227.t1;Parent=MS.gene062227.t1
Gene Sequence

>MS.gene062227

ATGGCATCATCATCATGTGTAGGAACTCTATTTTCCTCACAAACCAAACATTTGACTGCTACCAAGCCCAAGGTAAGTGTAACTTTGCCTTCAACAAGGAGAAGAGTTGTAAGAGCAGCTGTCACAGAGAAACTTGGCATCAAGATTGAGAGCAACCCTCCTGAGTCCAAGCTCACTCAACTTGGTGTTAGGCAGTGGCCCAAGTAAATACCACATTTCTTCATTATCTTGTTACTTTTTACCTCATCATGTCTATTATTCTAATGAAACTTGTTTCTTACATTTTTTCTCATGGTGGTTAGATTATGATTTTTATTTTAATGAAAAGGGTAAATTTTTTAATTTATGACTCATGCTATGTGCCCTATGCTAGTTGCTCTTCTAGTTAACGAGATTAATACACTATGGTATCAACAAATTCACATCGAACCATATATATGACTTTAACAGTAGTTAGGAGAAAATTTAAACGTTTTTTATGATTTGACGGTTATGATAGATTTAACAACATAAAAAATCTTTCACGGTGTGTATGAATTAAATCATTTTTTTTATATGAATCTGTTTTTGACTGTGTACACACATTTTTGGTGTAAATAAAAACAGATGGGGATGCCCTCCAAGTAATTTTCCATGGACATATGATGCAACAGAGACATGCTATCTTTTGGAAGGAAAAGTGAAGGTCACTCCTAGTGGGGCTAATGAGGCAGTTGAAATTTCTGCTGGTGATTTGGTTGTTTTCCCAAAAGGGATGAGTTGTACTTGGGATGTTTCTGTTGGTGTGGATAAGCACTATAAATTTGAATAA

Protein sequence

>MS.gene062227.t1

MASSSCVGTLFSSQTKHLTATKPKVSVTLPSTRRRVVRAAVTEKLGIKIESNPPESKLTQLGVRQWPKWGCPPSNFPWTYDATETCYLLEGKVKVTPSGANEAVEISAGDLVVFPKGMSCTWDVSVGVDKHYKFE