AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072665


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072665.t1 MTR_7g110130 93.382 136 8 1 1 135 131 266 2.10e-85 250
MS.gene072665.t1 MTR_2g090165 82.963 135 23 0 1 135 130 264 3.91e-76 227
MS.gene072665.t1 MTR_7g110160 85.039 127 19 0 1 127 112 238 8.83e-74 220
MS.gene072665.t1 MTR_7g110140 68.382 136 40 1 1 133 130 265 4.70e-60 186
MS.gene072665.t1 MTR_6g035395 63.158 133 49 0 1 133 116 248 4.97e-54 170
MS.gene072665.t1 MTR_4g052320 50.794 126 58 2 7 130 138 261 5.09e-35 122
MS.gene072665.t1 MTR_6g023590 45.968 124 67 0 1 124 139 262 2.51e-34 120
MS.gene072665.t1 MTR_4g052340 52.459 122 55 1 7 128 144 262 4.72e-34 119
MS.gene072665.t1 MTR_4g052350 49.612 129 62 1 7 135 144 269 7.89e-34 119
MS.gene072665.t1 MTR_7g105570 50.000 108 54 0 20 127 113 220 2.32e-33 117
MS.gene072665.t1 MTR_6g023950 46.774 124 66 0 1 124 139 262 9.13e-33 116
MS.gene072665.t1 MTR_7g105460 49.074 108 55 0 20 127 156 263 1.04e-32 116
MS.gene072665.t1 MTR_7g105580 46.667 135 70 2 1 134 115 248 1.12e-32 115
MS.gene072665.t1 MTR_4g052300 49.206 126 59 2 7 130 138 260 6.28e-32 114
MS.gene072665.t1 MTR_6g023990 44.355 124 69 0 1 124 140 263 3.44e-31 112
MS.gene072665.t1 MTR_7g071680 45.000 140 60 1 8 130 161 300 8.99e-31 112
MS.gene072665.t1 MTR_6g023610 44.715 123 68 0 1 123 139 261 1.73e-30 110
MS.gene072665.t1 MTR_6g023910 43.902 123 69 0 1 123 139 261 2.07e-29 107
MS.gene072665.t1 MTR_6g023600 44.355 124 69 0 1 124 133 256 2.53e-29 107
MS.gene072665.t1 MTR_6g023600 44.355 124 69 0 1 124 139 262 2.60e-29 107
MS.gene072665.t1 MTR_7g107670 43.443 122 64 1 8 124 163 284 5.22e-28 104
MS.gene072665.t1 MTR_3g020670 43.802 121 62 1 20 134 160 280 6.25e-26 99.0
MS.gene072665.t1 MTR_4g078630 40.141 142 78 2 1 135 141 282 1.19e-25 98.2
MS.gene072665.t1 MTR_7g083870 41.985 131 70 3 8 132 166 296 8.08e-24 93.6
MS.gene072665.t1 MTR_7g105590 51.613 62 29 1 1 62 133 193 8.67e-13 62.8
MS.gene072665.t1 MTR_3g113870 48.276 58 30 0 8 65 151 208 2.94e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072665.t1 AT2G47140 65.873 126 43 0 1 126 130 255 1.77e-54 171
MS.gene072665.t1 AT3G29250 58.268 127 53 0 1 127 130 256 5.54e-45 147
MS.gene072665.t1 AT3G29250 58.268 127 53 0 1 127 168 294 7.81e-45 148
MS.gene072665.t1 AT3G29250 58.268 127 53 0 1 127 166 292 8.93e-45 148
MS.gene072665.t1 AT2G47130 58.268 127 52 1 1 127 130 255 2.00e-44 146
MS.gene072665.t1 AT2G47120 55.814 129 51 3 1 127 131 255 1.27e-43 144
MS.gene072665.t1 AT2G47120 55.814 129 51 3 1 127 130 254 1.35e-43 144
MS.gene072665.t1 AT3G29260 58.594 128 50 2 1 127 130 255 1.55e-41 139
MS.gene072665.t1 AT3G51680 49.640 139 61 1 1 130 163 301 5.74e-34 120
MS.gene072665.t1 AT1G52340 47.143 140 67 2 1 134 147 285 6.27e-29 107
MS.gene072665.t1 AT3G26760 37.500 128 73 1 8 128 167 294 3.36e-24 94.7
MS.gene072665.t1 AT4G03140 39.098 133 75 2 1 128 202 333 5.77e-24 94.7
MS.gene072665.t1 AT3G26770 42.636 129 61 3 8 127 172 296 1.60e-22 90.5
MS.gene072665.t1 AT3G42960 45.926 135 64 4 8 133 136 270 9.29e-22 87.8
MS.gene072665.t1 AT2G47150 35.433 127 49 3 1 127 105 198 6.90e-15 68.6

Find 28 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAAAGGAATTACATTAA+AGG 0.203968 7.3:-5014786 MS.gene072665:CDS
AGTGTAGCTGCTTCTGTTGC+TGG 0.306606 7.3:-5015001 MS.gene072665:CDS
ACATCCAAACATGGTCTCTT+AGG 0.311178 7.3:-5014956 MS.gene072665:CDS
TTATATTAGTGGACACAATT+TGG 0.312298 7.3:-5014714 MS.gene072665:CDS
TACACTGGCAGTACAAATTA+TGG 0.348031 7.3:+5015018 None:intergenic
GCTGCTTCTGTTGCTGGAAC+TGG 0.384524 7.3:-5014995 MS.gene072665:CDS
GAAGAATCAGCTTATATTAG+TGG 0.431042 7.3:-5014725 MS.gene072665:CDS
TGCGGTGAGCTTGGAGCTTA+CGG 0.431271 7.3:-5014917 MS.gene072665:CDS
CGTTCAACGTGCGGTGAGCT+TGG 0.449623 7.3:-5014926 MS.gene072665:CDS
AAGTCCTAAGAGACCATGTT+TGG 0.467093 7.3:+5014952 None:intergenic
AATGATTCTGCTAATTTGAA+AGG 0.470724 7.3:-5014800 MS.gene072665:CDS
AGCTGCTCTCTTTCTTGCAT+CGG 0.473087 7.3:-5014747 MS.gene072665:CDS
CACAATTTGGTGGTCGATGG+AGG 0.510608 7.3:-5014701 MS.gene072665:CDS
GTGAGTGAAGTTGAAGCTAT+GGG 0.510902 7.3:-5014824 MS.gene072665:CDS
AGAGGCGTGGCAACAGCATA+CGG 0.535077 7.3:+5014872 None:intergenic
AGCAACAGAAGCAGCTACAC+TGG 0.548413 7.3:+5015003 None:intergenic
GCTTCTGTTGCTGGAACTGG+TGG 0.550638 7.3:-5014992 MS.gene072665:CDS
GGTGAGTGAAGTTGAAGCTA+TGG 0.560707 7.3:-5014825 MS.gene072665:CDS
TATTAGTGGACACAATTTGG+TGG 0.561555 7.3:-5014711 MS.gene072665:CDS
GGACACAATTTGGTGGTCGA+TGG 0.563116 7.3:-5014704 MS.gene072665:CDS
TGCATGTAGAGCACTGAACA+TGG 0.590712 7.3:-5014849 MS.gene072665:CDS
TTCAGTGCTCTACATGCAAG+AGG 0.603585 7.3:+5014854 None:intergenic
GATTATGTCACATCCAAACA+TGG 0.609752 7.3:-5014965 MS.gene072665:CDS
TGCTCTACATGCAAGAGGCG+TGG 0.617171 7.3:+5014859 None:intergenic
GGACTTGTACGTTCAACGTG+CGG 0.641327 7.3:-5014935 MS.gene072665:CDS
CAGTACAAATTATGGATCCG+CGG 0.653793 7.3:+5015026 None:intergenic
TGAGTGAAGTTGAAGCTATG+GGG 0.654036 7.3:-5014823 MS.gene072665:CDS
ATGTAGAGCACTGAACATGG+AGG 0.811309 7.3:-5014846 MS.gene072665:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGAAAGGAATTACATTAA+AGG - chr7.3:5014915-5014934 MS.gene072665:CDS 20.0%
! AATGATTCTGCTAATTTGAA+AGG - chr7.3:5014901-5014920 MS.gene072665:CDS 25.0%
!! TTATATTAGTGGACACAATT+TGG - chr7.3:5014987-5015006 MS.gene072665:CDS 25.0%
GAAGAATCAGCTTATATTAG+TGG - chr7.3:5014976-5014995 MS.gene072665:CDS 30.0%
GATTATGTCACATCCAAACA+TGG - chr7.3:5014736-5014755 MS.gene072665:CDS 35.0%
TACACTGGCAGTACAAATTA+TGG + chr7.3:5014686-5014705 None:intergenic 35.0%
! TATTAGTGGACACAATTTGG+TGG - chr7.3:5014990-5015009 MS.gene072665:CDS 35.0%
AAGTCCTAAGAGACCATGTT+TGG + chr7.3:5014752-5014771 None:intergenic 40.0%
ACATCCAAACATGGTCTCTT+AGG - chr7.3:5014745-5014764 MS.gene072665:CDS 40.0%
CAGTACAAATTATGGATCCG+CGG + chr7.3:5014678-5014697 None:intergenic 40.0%
GTGAGTGAAGTTGAAGCTAT+GGG - chr7.3:5014877-5014896 MS.gene072665:CDS 40.0%
TGAGTGAAGTTGAAGCTATG+GGG - chr7.3:5014878-5014897 MS.gene072665:CDS 40.0%
AGCTGCTCTCTTTCTTGCAT+CGG - chr7.3:5014954-5014973 MS.gene072665:CDS 45.0%
GGTGAGTGAAGTTGAAGCTA+TGG - chr7.3:5014876-5014895 MS.gene072665:CDS 45.0%
TTCAGTGCTCTACATGCAAG+AGG + chr7.3:5014850-5014869 None:intergenic 45.0%
! ATGGTGGAGAGAAAAATCCG+CGG - chr7.3:5014658-5014677 MS.gene072665:CDS 45.0%
!! ATGTAGAGCACTGAACATGG+AGG - chr7.3:5014855-5014874 MS.gene072665:CDS 45.0%
!! TGCATGTAGAGCACTGAACA+TGG - chr7.3:5014852-5014871 MS.gene072665:CDS 45.0%
AGCAACAGAAGCAGCTACAC+TGG + chr7.3:5014701-5014720 None:intergenic 50.0%
AGTGTAGCTGCTTCTGTTGC+TGG - chr7.3:5014700-5014719 MS.gene072665:CDS 50.0%
GGACTTGTACGTTCAACGTG+CGG - chr7.3:5014766-5014785 MS.gene072665:CDS 50.0%
! CACAATTTGGTGGTCGATGG+AGG - chr7.3:5015000-5015019 MS.gene072665:CDS 50.0%
! GGACACAATTTGGTGGTCGA+TGG - chr7.3:5014997-5015016 MS.gene072665:CDS 50.0%
GCTGCTTCTGTTGCTGGAAC+TGG - chr7.3:5014706-5014725 MS.gene072665:CDS 55.0%
GCTTCTGTTGCTGGAACTGG+TGG - chr7.3:5014709-5014728 MS.gene072665:CDS 55.0%
TGCGGTGAGCTTGGAGCTTA+CGG - chr7.3:5014784-5014803 MS.gene072665:CDS 55.0%
TGCTCTACATGCAAGAGGCG+TGG + chr7.3:5014845-5014864 None:intergenic 55.0%
! AGAGGCGTGGCAACAGCATA+CGG + chr7.3:5014832-5014851 None:intergenic 55.0%
CGTTCAACGTGCGGTGAGCT+TGG - chr7.3:5014775-5014794 MS.gene072665:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 5014658 5015065 5014658 ID=MS.gene072665
chr7.3 mRNA 5014658 5015065 5014658 ID=MS.gene072665.t1;Parent=MS.gene072665
chr7.3 exon 5014658 5015065 5014658 ID=MS.gene072665.t1.exon1;Parent=MS.gene072665.t1
chr7.3 CDS 5014658 5015065 5014658 ID=cds.MS.gene072665.t1;Parent=MS.gene072665.t1
Gene Sequence

>MS.gene072665

ATGGTGGAGAGAAAAATCCGCGGATCCATAATTTGTACTGCCAGTGTAGCTGCTTCTGTTGCTGGAACTGGTGGTCATGATTATGTCACATCCAAACATGGTCTCTTAGGACTTGTACGTTCAACGTGCGGTGAGCTTGGAGCTTACGGAATTAGAGTCAATTCAATCTCACCGTATGCTGTTGCCACGCCTCTTGCATGTAGAGCACTGAACATGGAGGTGAGTGAAGTTGAAGCTATGGGGAATGATTCTGCTAATTTGAAAGGAATTACATTAAAGGCTACTCATATTGCAGAAGCTGCTCTCTTTCTTGCATCGGAAGAATCAGCTTATATTAGTGGACACAATTTGGTGGTCGATGGAGGCTTTTCTGTTATTAATAGTTGTGTTCCTACTATTAAAAAATAA

Protein sequence

>MS.gene072665.t1

MVERKIRGSIICTASVAASVAGTGGHDYVTSKHGLLGLVRSTCGELGAYGIRVNSISPYAVATPLACRALNMEVSEVEAMGNDSANLKGITLKATHIAEAALFLASEESAYISGHNLVVDGGFSVINSCVPTIKK